@article{edens_parkhurst_qureshi_casas_havenstein_1997, title={Atypical Escherichia coli strains and their association with poult enteritis and mortality syndrome}, volume={76}, ISSN={["0032-5791"]}, DOI={10.1093/ps/76.7.952}, abstractNote={
 Abstract
 
 To date, no definitive etiology has been described for Poult Enteritis and Mortality Syndrome (PEMS). However, two atypical Escherichia coli colony types are isolated consistently from moribund and dead poults afflicted with PEMS. To test the infectivity of these E. coli strains, poults were placed into floor pens in three isolation treatment rooms: 1) Control: no bacterial challenge, 2) E. coli colony Types 1 or 2 posthatch oral challenge: 10(8) cfu/per poult at 1 d, and 3) E. coli colony Types 1 or 2 posthatch oral challenge: 10(8) cfu/per poult at 6 d. Daily intramuscular injections of cyclophosphamide (100 micrograms per poult) from 1 to 5 d posthatch were given to half of the poults in each treatment. Atypical E. coli challenge caused BW depression, and cyclophosphamide treatment exacerbated the response. All E. coli-challenged poults developed diarrhea similar to PEMS. Mortality was increased by both atypical E. coli colony types, but at 21 d E. coli colony Type 2 caused greater mortality than colony Type 1. With cyclophosphamide treatment, mortality was exacerbated with both colony types, but colony Type 2 at 1 d caused the greatest mortality. Ultrastructural damage to ileum epithelium cell microvilli and subcellular organelles indicated that part of the BW depression could be attributed to malabsorption of nutrients. It was concluded that the atypical E. coli colony Types 1 and 2 play a significant role in the PEMS disease.
 
}, number={7}, journal={POULTRY SCIENCE}, author={Edens, FW and Parkhurst, CR and Qureshi, MA and Casas, IA and Havenstein, GB}, year={1997}, month={Jul}, pages={952–960} }
 @article{edens_qureshi_parkhurst_qureshi_havenstein_casas_1997, title={Characterization of two Escherichia coli isolates associated with poult enteritis and mortality syndrome}, volume={76}, ISSN={["0032-5791"]}, DOI={10.1093/ps/76.12.1665}, abstractNote={Two colonial types (1 and 2) of Escherichia coli are represented predominantly in cultures isolated from turkey poults with poult enteritis and mortality syndrome (PEMS). Biotype codes determined using two systems (BBL: 36570 and 34560 for colony types 1 and 2, respectively; API-20E: 5144572 and 5144512 for colony types 1 and 2, respectively) clearly establish these organisms as E. coli. These isolates were not clearly divergent from the general profile for E. coli, but colony type 2 differs from colony type 1 with regard to its negative reactions for ornithine decarboxylase and the fermentation of dulcitol, rhamnose, sucrose, and melibiose, suggesting that it is atypical. Colony type 1 is nonserotypable and nonmotile, whereas colony type 2 is serotyped as O136: motile because it has H antigens associated with flagella. Capsular antigens were not found, but thin capsules were seen on cells from both colony types in stained preparations. Cultural morphology was different with colony type 1 having a circular, mucoid, raised morphology and colony type 2 having an irregular, flat, rough morphology. Colony type 1 has a doubling time at 37 C of about 20 min, whereas colony type 2 doubles in 30 min. Furthermore, colony type 1 is a potent colicin producer, but colony type 2 is not a colicin producer. Both E. coli isolates have resistance profiles for multiple antibiotics. Each strain responds to third generation fluoroquinolone antibiotics by changing their biotypes and become resistant after culturing once in their presence. These E. coli are proposed as possible etiological links in the complex series of events that take place in poults susceptible to PEMS.}, number={12}, journal={POULTRY SCIENCE}, author={Edens, FW and Qureshi, RA and Parkhurst, CR and Qureshi, MA and Havenstein, GB and Casas, IA}, year={1997}, month={Dec}, pages={1665–1673} }
 @misc{edens_parkhurst_casas_dobrogosz_1997, title={Principles of ex ovo competitive exclusion and in ovo administration of Lactobacillus reuteri}, volume={76}, ISSN={["1525-3171"]}, DOI={10.1093/ps/76.1.179}, abstractNote={The data that have been presented indicate that the in ovo use of competitive exclusion (CE) agents is feasible for both chickens and turkeys. However, there are many pitfalls that await the use of in ovo application of CE agents, including the use of nonspecies-specific intestinal microbes and the use of harmful proteolytic, gas-producing and toxin-producing intestinal microbes. Of the potential CE agents that have posthatch application, only Lactobacillus reuteri has been shown to be safe and effective in terms of not affecting hatchability and in having a prolonged effect in the hatched chick or poult. Lactobacillus reuteri administration in ovo increases its rate of intestinal colonization and decreases the colonization of Salmonella and Escherichia coli in both chicks and poults. Additionally, mortality due to in-hatcher exposure to E. coli or Salmonella is reduced with in ovo L. reuteri. Use of antibiotics in ovo may preclude the use of co-administered CE agents, but Gentamicin and L. reuteri are a compatible mixture when administered in ovo in separate compartments. Nevertheless, the intestinal morphology can be affected by both the CE agent and by antibiotics. Lactobacillus reuteri both in ovo and ex ovo will increase villus height and crypt depth, and Gentamicin in ovo causes a shortening and blunting of the villus. Both Gentamicin and L. reuteri in ovo suppress potentially pathogenic enteric microbes, but with diminished antibiotic effects shortening and blunting of the intestinal villi does not correct itself. Goblet cell numbers increase significantly on the ileum villus of chicks treated with Gentamicin in ovo, and this is presumably due to the increase in potentially pathogenic bacteria in the intestinal tract. Diminishing antibiotic effects posthatch would then negatively affect the absorption of nutrients and reduce growth at least in a transitory manner. Thus, L reuteri administration in ovo singly or in combination with Gentamicin followed by L reuteri via drinking water or feed appears to have potential to control many enteric pathogens in poultry. Additional work in the use of in ovo CE cultures is mandated because there is a world-wide movement to reduce antibiotic use in poultry due to increased microbial resistance to antibiotics. Use of naturally occurring intestinal bacterial cultures, either in mixed culture or as single well-defined cultures, has potential for immediate use in the poultry industry.}, number={1}, journal={POULTRY SCIENCE}, author={Edens, FW and Parkhurst, CR and Casas, IA and Dobrogosz, WJ}, year={1997}, month={Jan}, pages={179–196} }