@article{sharkey_agusta_aroche_nissan_peterson_saha_twiddy_bozkurt_dickey_velev_et al._2025, title={Toward Wearable Biosensing and Rapid Diagnostics via Extraction of Dermal Interstitial Fluid}, volume={8}, DOI={10.1109/biosensors65002.2025.11239294}, abstractNote={Wearable biosensing platforms for rapid, minimally invasive biomarker monitoring hold great promise for data-driven healthcare and lifestyle management. This work presents a microneedle-based sampling system for the minimally invasive extraction and analysis of interstitial fluid biomarkers. The sampling system integrates hydrogel-forming microneedle patches with an osmotic pump and paper-based fluidics to efficiently collect dermal interstitial fluid. To demonstrate rapid diagnostics, electrochemical biosensors are incorporated for the detection of cortisol and lactate. Using in vitro skin models, the system demonstrated accurate analyte quantification, with enhanced cortisol detection achieved through pretreatment of paper fluidics with buffering compounds. The platform’s suitability for wearable applications is supported by its rapid time-to-result, physiologically relevant dynamic ranges, and modular design for multianalyte detection.}, author={Sharkey, Christopher T. and Agusta, Isabella G. and Aroche, Angélica F. and Nissan, Hannah E. and Peterson, Kaila L. and Saha, Tamoghna and Twiddy, Jack and Bozkurt, Alper and Dickey, Michael D. and Velev, Orlin D. and et al.}, year={2025}, month={Aug} }