Production of Chick Germline Chimeras from Fluorescence-Activated Cell-Sorted Gonocytes
Mozdziak, P. E., Wysocki, R., Angerman-Stewart, J., Pardue, S. L., & Petitte, J. N. (2006, October 1). Poultry Science, Vol. 10, pp. 1764–1768.
author keywords: gonad; stage-specific embryonic antigen-1; testes; fluorescence-activated cell sorting; primordial germ cell
MeSH headings : Animals; Cell Separation / methods; Cell Separation / veterinary; Chick Embryo / cytology; Chick Embryo / embryology; Chick Embryo / metabolism; Chimera / embryology; Embryo Culture Techniques / veterinary; Female; Flow Cytometry; Germ Cells / cytology; Germ Cells / transplantation; Male; Testis / cytology; Testis / embryology
topics (OpenAlex): Animal Genetics and Reproduction; CRISPR and Genetic Engineering; RNA Interference and Gene Delivery
TL;DR:
It was demonstrated that gonocytes isolated from stage 27 embryonic gonads can be introduced into embryos at an earlier stage of development, and the introduced gonocytes can fully participate in germline development.
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Sources: Web Of Science, ORCID, NC State University Libraries