Works (133)

Updated: May 15th, 2024 04:36

2023 journal article

A LGR5 reporter pig model closely resembles human intestine for improved study of stem cells in disease

FASEB JOURNAL, 37(6).

By: C. Schaaf n, K. Polkoff n, A. Carter n, A. Stewart n, B. Sheahan n, J. Freund n, J. Ginzel*, J. Snyder* ...

author keywords: APC mutation; colorectal cancer; intestine; Lgr5; porcine; stem cell; transgenic large animal
MeSH headings : Humans; Swine; Animals; Mice; In Situ Hybridization, Fluorescence; Intestines; Stem Cells; Ileum; Colon; Coleoptera; Green Fluorescent Proteins / genetics; Receptors, G-Protein-Coupled / genetics
TL;DR: This study validates ISC isolation in a new porcine Leucine Rich Repeat Containing G Protein‐Coupled Receptor 5 (LGR5) reporter line and demonstrates the use of these pigs as a novel colorectal cancer model. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: June 12, 2023

2023 journal article

A postnatal network of co-hepato/pancreatic stem/progenitors in the biliary trees of pigs and humans

NPJ REGENERATIVE MEDICINE, 8(1).

By: W. Zhang*, X. Wang*, G. Lanzoni, E. Wauthier*, S. Simpson n, J. Ezzell*, A. Allen*, C. Suitt ...

TL;DR: Hepato/pancreatic multipotentiality was demonstrated by the stem/progenitors’ responses under distinct ex vivo conditions or in vivo when patch grafted as organoids onto the liver versus the pancreas. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 21, 2023

2023 article

Adrenomedullin Stimulates Proliferation, Migration and Adhesion of Porcine Trophectoderm Cells Via CALCRL-AKT-TSC2-MTORC1 Cell Signaling Pathway

Liu, B., Paudel, S., Flowers, W. L., Piedrahita, J. A., & Wang, X. (2023, May). JOURNAL OF ANIMAL SCIENCE, Vol. 101.

By: B. Liu n, S. Paudel n, W. Flowers n, J. Piedrahita n & X. Wang n

author keywords: adrenomedullin; peri-implantation; pig conceptus
TL;DR: Results suggest that porcine ADM in histotroph act on its receptor component CALCRL to activate AKT-TSC2-MTOR, particularly MTORC1 signaling cascade, leading to elongation, migration and attachment of conceptuses. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: March 4, 2024

2023 article

Structure-guided AAV capsid evolution strategies for enhanced CNS gene delivery

Gonzalez, T. J., Mitchell-Dick, A., Blondel, L. O., Fanous, M. M., Hull, J. A., Oh, D. K., … Asokan, A. (2023, September 21). NATURE PROTOCOLS, Vol. 9.

By: T. Gonzalez*, A. Mitchell-Dick*, L. Blondel*, M. Fanous*, J. Hull*, D. Oh*, S. Moller-Tank*, R. Rivera*, J. Piedrahita n, A. Asokan*

TL;DR: A detailed protocol describing two distinct evolution strategies using structurally divergent AAV serotypes as templates, exemplified by improving CNS gene transfer efficiency in vivo, can be adapted to expand the AAV vector toolkit for genetic manipulation of animal models and development of human gene therapies. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: October 16, 2023

2023 article

Uterine histotroph and conceptus development: III. Adrenomedullin stimulates proliferation, migration and adhesion of porcine trophectoderm cells via AKT-TSC2-MTOR cell signaling pathway

Liu, B., Paudel, S., Flowers, W. L., Piedrahita, J. A., & Wang, X. (2023, April 10). AMINO ACIDS, Vol. 4.

By: B. Liu n, S. Paudel n, W. Flowers n, J. Piedrahita n & X. Wang n

author keywords: Adrenomedullin; Trophectoderm cells; MTOR; Pigs; Implantation
MeSH headings : Pregnancy; Female; Swine; Animals; Proto-Oncogene Proteins c-akt / genetics; Proto-Oncogene Proteins c-akt / metabolism; Adrenomedullin / pharmacology; Signal Transduction; TOR Serine-Threonine Kinases / genetics; TOR Serine-Threonine Kinases / metabolism; Cell Proliferation; Sirolimus / pharmacology
TL;DR: Results suggest that porcine ADM in histotroph acts on its receptor component CALCRL to activate AKT-TSC2-MTOR, particularly MTORC1 signaling cascade, leading to elongation, migration and attachment of conceptuses. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: April 24, 2023

2022 journal article

Age- and Sex-Specific Joint Biomechanics in Response to Partial and Complete Anterior Cruciate Ligament Injury in the Porcine Model

JOURNAL OF ATHLETIC TRAINING, 57(9-10), 978–989.

By: D. Howe n, S. Cone n, J. Piedrahita n, J. Spang* & M. Fisher n

author keywords: knee; transection studies; sex factors
MeSH headings : Male; Female; Humans; Animals; Swine; Anterior Cruciate Ligament Injuries / surgery; Anterior Cruciate Ligament / surgery; Anterior Cruciate Ligament / physiology; Biomechanical Phenomena; Joint Instability; Knee Joint; Cadaver
TL;DR: Increased forces in the MCL and medial meniscus after ACL transection suggest that rehabilitation protocols may need to focus on protecting these tissues, and operative treatment should be considered in active, late adolescent patients. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
5. Gender Equality (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 6, 2023

2022 journal article

Allogeneic and xenogeneic lymphoid reconstitution in a RAG2(-/-)IL2RG(y/-) severe combined immunodeficient pig: A preclinical model for intrauterine hematopoietic transplantation

FRONTIERS IN VETERINARY SCIENCE, 9.

By: R. Sper n, J. Proctor n, O. Lascina n, L. Guo n, K. Polkoff n, T. Kaeser n, S. Simpson n, L. Borst n ...

author keywords: severe combined immunodeficiency; pig; xenotransplantation; allotransplantation; hematopoietic; RAG2; IL2RG; transgenic
TL;DR: The data shows that the pig thymus provides a microenvironment conducive to engraftment, survival and development of human T-cells and provides evidence that the developing T-cell compartment can be populated to a significant extent by human cells in large animals. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: November 14, 2022

2022 journal article

Cross-species evolution of a highly potent AAV variant for therapeutic gene transfer and genome editing

NATURE COMMUNICATIONS, 13(1).

By: T. Gonzalez*, K. Simon*, L. Blondel*, M. Fanous*, A. Roger*, M. Maysonet, G. Devlin*, T. Smith* ...

MeSH headings : Animals; Dependovirus / metabolism; Gene Editing; Genetic Therapy; Genetic Vectors / genetics; Humans; Macaca / genetics; Mice; Swine; Transduction, Genetic
TL;DR: A highly potent new variant of AAV, AAV.cc47, is described with enhanced transduction efficiency over AAV serotype 9 and its efficacy is shown in different mouse models, pigs and non-human primates. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: October 17, 2022

2022 article

Donor Age and Time in Culture Affect Dermal Fibroblast Contraction in an In Vitro Hydrogel Model

Detwiler, A., Polkoff, K., Gaffney, L., Freytes, D. O., & Piedrahita, J. A. (2022, August 4). TISSUE ENGINEERING PART A, Vol. 8.

By: A. Detwiler n, K. Polkoff n, L. Gaffney n, D. Freytes n & J. Piedrahita n

author keywords: cell proliferation; collagen; dermis; extracellular matrix; fibroblasts; hydrogels; skin; wound healing
MeSH headings : Adult; Infant, Newborn; Humans; Swine; Animals; Hydrogels / pharmacology; Reproducibility of Results; Wound Healing; Collagen / chemistry; Fibroblasts
TL;DR: Results show how cellular responses in a hydrogel scaffold differ based on donor age and time in culture in vitro, and suggest that consistency in the cellular component of bioengineered skin products could be beneficial in the biomanufacturing of consistent, reliable skin grafts and graft in vivo models. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 29, 2022

2022 journal article

LGR5 is a conserved marker of hair follicle stem cells in multiple species and is present early and throughout follicle morphogenesis

SCIENTIFIC REPORTS, 12(1).

By: K. Polkoff n, N. Gupta n, A. Green n, Y. Murphy n, J. Chung n, K. Gleason n, S. Simpson*, D. Walker n, B. Collins n, J. Piedrahita n

MeSH headings : Animals; Animals, Genetically Modified; Biomarkers / metabolism; Hair Follicle / metabolism; Humans; Morphogenesis; Receptors, G-Protein-Coupled / genetics; Receptors, G-Protein-Coupled / metabolism; Skin; Stem Cells / metabolism; Swine
TL;DR: It is demonstrated that LGR5 is a marker of hair follicle stem cells across species in homeostasis and development and the strong similarities and important differences in expression patterns, gene expression profiles, and developmental processes between species are reported. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: June 20, 2022

2022 article

Multiscale Anisotropic Tissue Biofabrication via Bulk Acoustic Patterning of Cells and Functional Additives in Hybrid Bioinks

Chansoria, P., Asif, S., Gupta, N., Piedrahita, J., & Shirwaiker, R. A. (2022, January 27). ADVANCED HEALTHCARE MATERIALS, Vol. 1.

By: P. Chansoria n, S. Asif n, N. Gupta n, J. Piedrahita n & R. Shirwaiker n

author keywords: 3D cell patterning; biofabrication; bioprinting; GelMA; hybrid bioinks; standing bulk acoustic waves; ultrasound
MeSH headings : Acoustics; Anisotropy; Bioprinting; Gelatin / chemistry; Humans; Hydrogels / chemistry; Methacrylates; Printing, Three-Dimensional; Tissue Engineering; Tissue Scaffolds / chemistry
TL;DR: A highly versatile ultrasound‐assisted biofabrication (UAB) approach is demonstrated that utilizes radiation forces generated by superimposing ultrasonic bulk acoustic waves to rapidly organize arrays of cells and other biomaterial additives within single and multilayered hydrogel constructs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
9. Industry, Innovation and Infrastructure (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: February 7, 2022

2022 journal article

Ontogeny of cellular organization and LGR5 expression in porcine cochlea revealed using tissue clearing and 3D imaging

ISCIENCE, 25(8).

By: A. Moatti n, C. Li n, S. Sivadanam n, Y. Cai n, J. Ranta n, J. Piedrahita n, A. Cheng*, F. Ligler n, A. Greenbaum n

TL;DR: Data reveal that hair cell organization, inner ear morphology, cellular cartography in the organ of Corti, and spatiotemporal expression of LGR5 are dynamic over developmental stages in a pattern not previously documented. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: December 19, 2022

2022 article

The use of autologous skeletal muscle progenitor cells for adjunctive treatment of presumptive urethral sphincter mechanism incompetence in female dogs

Vaden, S. L., Mathews, K. G., Yoo, J., Williams, J. K., Harris, T., Secoura, P., … Piedrahita, J. (2022, August 5). JOURNAL OF VETERINARY INTERNAL MEDICINE, Vol. 8.

By: S. Vaden n, K. Mathews n, J. Yoo*, J. Williams*, T. Harris n, P. Secoura n, J. Robertson n, K. Gleason n, H. Reynolds n, J. Piedrahita n

Contributors: J. Robertson n

author keywords: canine; regenerative medicine; stem cell; urinary incontinence
MeSH headings : Animals; Dog Diseases / surgery; Dogs; Female; Muscle, Skeletal; Stem Cells; Urethra / surgery; Urinary Incontinence / therapy; Urinary Incontinence / veterinary
TL;DR: Urethral submucosal injection of autologous skeletal muscle progenitor cells (skMPC) can be used adjunctively to improve continence in dogs with difficult to manage USMI. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 26, 2022

2021 journal article

Characterizing the Effects of Synergistic Thermal and Photo-Cross-Linking during Biofabrication on the Structural and Functional Properties of Gelatin Methacryloyl (GeIMA) Hydrogels

ACS BIOMATERIALS SCIENCE & ENGINEERING, 7(11), 5175–5188.

By: P. Chansoria n, S. Asif n, K. Polkoff n, J. Chung n, J. Piedrahita n & R. Shirwaiker n

author keywords: GelMA; thermo-reversible cross-linking; photo-cross-linking; biofabrication; tissue engineering; CryoSEM; qPCR
MeSH headings : Biocompatible Materials; Gelatin; Humans; Hydrogels; Methacrylates; Tissue Engineering
TL;DR: The finding that hydrogels processed at higher temperature possessed lower structural fidelity but resulted in more cell elongation and greater anisotropy over time demonstrate the significant impact of GelMA material formulation and processing conditions on the structural and functional properties of the hydrogel. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: November 29, 2021

2021 journal article

Patch grafting, strategies for transplantation of organoids into solid organs such as liver

BIOMATERIALS, 277.

By: W. Zhang*, G. Lanzoni*, H. Hani*, D. Overi*, V. Cardinale*, S. Simpson n, W. Pitman*, A. Allen* ...

author keywords: Liver; Biliary tree; Stem/progenitors; Cell therapies; Grafting; Matrix-metalloproteinases (MMPs); Hyaluronans
MeSH headings : Animals; Cell Differentiation; Hepatocytes; Liver; Organoids; Stem Cells; Swine
TL;DR: RNA-seq analyses revealed that engraftment correlated with expression of matrix-metalloproteinases (MMPs), especially secreted isoforms that were found expressed strongly by organoids, less so by MSCs, and minimally, if at all, by adult cells. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: October 4, 2021

2021 article

Sex-specific biomechanics and morphology of the anterior cruciate ligament during skeletal growth in a porcine model

Howe, D., Cone, S. G., Piedrahita, J. A., Collins, B., Fordham, L. A., Griffith, E. H., … Fisher, M. B. (2021, November 9). JOURNAL OF ORTHOPAEDIC RESEARCH, Vol. 11.

By: D. Howe n, S. Cone n, J. Piedrahita n, B. Collins n, L. Fordham*, E. Griffith n, J. Spang*, M. Fisher n

author keywords: anterior cruciate ligament; knee; porcine model; skeletal growth
MeSH headings : Adolescent; Aged; Animals; Anterior Cruciate Ligament; Anterior Cruciate Ligament Injuries; Biomechanical Phenomena; Child; Female; Humans; Joint Instability; Knee Joint; Male; Swine; Tibia
TL;DR: A porcine model is used to investigate the sex-specific morphology and biomechanics of the ACL and its bundles throughout skeletal growth and has implications for age and sex‐specific surgical intervention strategies and suggests the need for human studies. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
5. Gender Equality (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 15, 2021

2020 article

Clinical xenotransplantation of the heart: At the watershed

Platt, J. L., Piedrahita, J. A., & Cascalho, M. (2020, August). JOURNAL OF HEART AND LUNG TRANSPLANTATION, Vol. 39, pp. 758–760.

By: J. Platt*, J. Piedrahita n & M. Cascalho*

MeSH headings : Animals; Heart; Heart Transplantation; Primates; Swine; Transplantation, Heterologous
Sources: Web Of Science, NC State University Libraries
Added: August 10, 2020

2020 journal article

In Vitro Validation of Transgene Expression in Gene-Edited Pias Using CRISPR Transcriptional Activators

CRISPR JOURNAL, 3(5), 409–418.

By: K. Polkoff n, J. Chung n, S. Simpson*, K. Gleason n & J. Piedrahita n

MeSH headings : Animals; Animals, Genetically Modified / genetics; CRISPR-Associated Protein 9 / genetics; CRISPR-Associated Protein 9 / metabolism; Cells, Cultured; Clustered Regularly Interspaced Short Palindromic Repeats; Gene Editing / methods; Gene Expression; Nuclear Transfer Techniques; RNA, Guide, Kinetoplastida / genetics; RNA, Guide, Kinetoplastida / metabolism; Swine; Trans-Activators / genetics; Trans-Activators / metabolism; Transgenes
TL;DR: It is shown that CRISPR-dCas9 transcriptional activators can be used to validate functional transgene insertion in nonexpressing easily cultured cells such as fibroblasts and is a tool that could be used across disciplines and animal species to save time and resources by verifying expected outcomes of gene edits before generating live animals. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: November 24, 2020

2020 journal article

Joint laxity varies in response to partial and complete anterior cruciate ligament injuries throughout skeletal growth

JOURNAL OF BIOMECHANICS, 101.

By: S. Cone n, E. Lambeth n, J. Piedrahita n, J. Spang* & M. Fisher n

author keywords: Anterior cruciate ligament; Pediatric; Injury; Joint mechanics; Animal model
MeSH headings : Animals; Anterior Cruciate Ligament Injuries / complications; Biomechanical Phenomena; Female; Joint Instability / complications; Joint Instability / physiopathology; Rotation; Stifle / growth & development; Stifle / physiopathology; Swine
TL;DR: At all ages, the medial collateral ligament and the medial meniscus combined to resist the majority of applied anterior tibial load following complete ACL transection, adding to the current understanding of age-specific functional properties of both healthy and injured knees during skeletal growth. (via Semantic Scholar)
UN Sustainable Development Goal Categories
5. Gender Equality (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 23, 2020

2020 journal article

Three-dimensional imaging of intact porcine cochlea using tissue clearing and custom-built light-sheet microscopy

BIOMEDICAL OPTICS EXPRESS, 11(11), 6181–6196.

By: A. Moatti n, Y. Cai n, C. Li n, T. Sattler n, L. Edwards n, J. Piedrahita n, F. Ligler n, A. Greenbaum n

TL;DR: 3D microscopic analysis was performed on an intact cochlea of newborn and juvenile pigs with a volume up to ∼ 250 mm3 using the BoneClear tissue clearing technique, which renders the bone transparent, to support the growing effort to regenerate cochlear tissues and assist with basic research to advance cures for hearing impairments. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: December 11, 2020

2019 journal article

200 Developing exosomes as a mediator for CRISPR/Cas-9 delivery

Reproduction, Fertility and Development, 31(1), 225.

By: N. Gupta n, K. Polkoff n, L. Qiao n, K. Cheng n & J. Piedrahita n

TL;DR: This project uses exosomes as a delivery system for Cas9, exploiting their high uptake by target cells and their ability to avoid the immune system in vivo, and hypothesise that based on loading efficiency and target cell uptake, exosome will present a safe and efficient method for in vitro and in vivo delivery of Cas9. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 21, 2020

2019 journal article

Biomechanical Function and Size of the Anteromedial and Posterolateral Bundles of the ACL Change Differently with Skeletal Growth in the Pig Model

CLINICAL ORTHOPAEDICS AND RELATED RESEARCH, 477(9), 2161–2174.

By: S. Cone n, E. Lambeth, H. Ru*, L. Fordham, J. Piedrahita*, J. Spang, M. Fisher*

MeSH headings : Aging / physiology; Animals; Anterior Cruciate Ligament / physiology; Biomechanical Phenomena; Cadaver; Models, Animal; Musculoskeletal Development / physiology; Range of Motion, Articular / physiology; Rotation; Swine; Tibia / physiology; Torque
UN Sustainable Development Goal Categories
5. Gender Equality (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: February 17, 2020

2019 journal article

In Situ Joint Stiffness Increases During Skeletal Growth but Decreases Following Partial and Complete Anterior Cruciate Ligament Injury

JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME, 141(12).

By: S. Cone n, J. Piedrahita n, J. Spang* & M. Fisher n

TL;DR: This work furthers the understanding of the interaction between joint biomechanics and ACL function throughout growth and the impact of ACL injury in the skeletally immature joint. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
5. Gender Equality (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 27, 2020

2019 journal article

Tissue-specific changes in size and shape of the ligaments and tendons of the porcine knee during post-natal growth

PLOS ONE, 14(10).

By: S. Cone n, H. Piercy n, E. Lambeth n, H. Ru n, J. Piedrahita n, J. Spang*, L. Fordham*, M. Fisher n

MeSH headings : Animals; Anterior Cruciate Ligament / diagnostic imaging; Anterior Cruciate Ligament / growth & development; Female; Knee Joint / diagnostic imaging; Knee Joint / growth & development; Magnetic Resonance Imaging; Organ Specificity; Patellar Ligament / diagnostic imaging; Patellar Ligament / growth & development; Swine
TL;DR: The findings suggest that musculoskeletal soft tissue morphometry can vary within tissues of similar structure and within a single joint during post-natal growth. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: June 8, 2020

2018 journal article

High mobility group A2 (HMGA2) deficiency in pigs leads to dwarfism, abnormal fetal resource allocation, and cryptorchidism

Proceedings of the National Academy of Sciences, 115(21), 5420–5425.

By: J. Chung n, X. Zhang n, B. Collins n, R. Sper n, K. Gleason n, S. Simpson n, S. Koh n, J. Sommer n ...

author keywords: HMGA2; dwarfism; swine; gene editing; organ size
MeSH headings : Animals; Cryptorchidism / etiology; Cryptorchidism / pathology; Dwarfism / etiology; Dwarfism / pathology; Female; Fetal Diseases / etiology; Fetal Diseases / pathology; Genotype; HMGA2 Protein / deficiency; HMGA2 Protein / genetics; Litter Size; Male; Nuclear Transfer Techniques / veterinary; Pregnancy; Reproduction; Swine; Swine Diseases / etiology; Swine Diseases / pathology
TL;DR: The results show that the effect of HMGA2 with respect to growth regulation is highly conserved among mammals and opens up the possibility of regulating body and organ size in a variety of mammalian species including food and companion animals. (via Semantic Scholar)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2018 article

INTRODUCTION: Immune Relevant Animal Models: Opportunities and Challenges

ILAR JOURNAL, Vol. 59, pp. 209–210.

By: G. Jungersen* & J. Piedrahita n

author keywords: animal models; immunology; preclinical validity
MeSH headings : Allergy and Immunology; Animals; Disease Models, Animal; Inflammation
TL;DR: Assessment of immune-relevant animal models used in oncology, hematopoietic-, CAR-T cell- and xenotransplantation, adjuvants and infectious diseases, and immune privileged inflammation that are providing key insights into unmet human clinical needs are provided. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: December 2, 2019

2018 review

Xenotransplantation: Progress Along Paths Uncertain from Models to Application

[Review of ]. ILAR JOURNAL, 59(3), 286–308.

By: J. Platt*, M. Cascalho* & J. Piedrahita n

author keywords: adaptive immunity; clinical xenotransplantation; gene editing; innate immunity; molecular incompatibility; nonhuman primate; reverse xenograft; transgenic pig; xenotransplantation; zoonosis
MeSH headings : Adaptive Immunity; Animals; Gene Editing / methods; Genetic Engineering; Heterografts; Humans; Immunity, Innate / physiology; Primates; Stem Cells / metabolism; Swine
TL;DR: The last 2 decades have brought progressive advances in approaches that can be applied to genetic modification of large animals, which make it appropriate to consider the potential limitation of genetic engineering and of current models for advancing the clinical applications of xenotransplantation and reverse xenotranplantation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: December 2, 2019

2017 journal article

Animal Models in Tissue Engineering. Part I

Tissue Engineering Part C: Methods, 23(11), 641–642.

By: J. Piedrahita n & J. Williams*

author keywords: animal models; tissue engineering; regenerative medicine
MeSH headings : Animals; CRISPR-Cas Systems / genetics; Mice; Models, Animal; Rats; Sus scrofa; Tissue Engineering / methods; Translational Research, Biomedical
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2017 journal article

Animal Models in Tissue Engineering. Part II

Tissue Engineering Part C: Methods, 23(12), 827–828.

By: J. Piedrahita n & J. Williams*

MeSH headings : Animals; Models, Biological; Tissue Engineering / methods; Tissue Engineering / trends
TL;DR: Advances in areas such as 3D printing and bioreactor technologies now permit the in vitro development and testing of complex scaffold/cell composites, but in vivo testing remains critical for the critical efficacy and safety testing required for regulatory approval. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: July 27, 2019

2017 journal article

Generation of LIF-independent induced pluripotent stem cells from canine fetal fibroblasts

Theriogenology, 92, 75–82.

By: N. Gonçalves, F. Bressan*, K. Roballo, F. Meirelles*, P. Xavier, H. Fukumasu, C. Williams n, M. Breen n ...

author keywords: iPSC; Canine; Stem cells; Pluripotency; Cellular reprogramming
MeSH headings : Animals; Dogs; Fetus / cytology; Fibroblasts / cytology; Fibroblasts / drug effects; Fibroblasts / physiology; Gene Expression Regulation / physiology; Leukemia Inhibitory Factor / pharmacology; Mice; Mice, Inbred BALB C; Mice, Nude; Pluripotent Stem Cells / physiology; Polymerase Chain Reaction / veterinary
TL;DR: This report is the first to describe the ability to reprogram canine somatic cells via lentiviral vectors without supplementation and with resistance to enzymatic action, thereby demonstrating the pluripotency of these cell lines. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2017 journal article

Generation of a Stable Transgenic Swine Model Expressing a Porcine Histone 2B-eGFP Fusion Protein for Cell Tracking and Chromosome Dynamics Studies

PLOS ONE, 12(1), e0169242.

By: R. Sper n, S. Koh*, X. Zhang n, S. Simpson n, B. Collins n, J. Sommer n, R. Petters n, I. Caballero n, J. Platt*, J. Piedrahita n

Ed(s): I. Polejaeva

MeSH headings : Animals; Cell Tracking / methods; Chromosomes, Mammalian / genetics; Chromosomes, Mammalian / metabolism; Female; Green Fluorescent Proteins / biosynthesis; Green Fluorescent Proteins / genetics; Histones / biosynthesis; Histones / genetics; Male; Organisms, Genetically Modified / genetics; Organisms, Genetically Modified / metabolism; Recombinant Fusion Proteins / biosynthesis; Recombinant Fusion Proteins / genetics; Swine / genetics; Swine / metabolism
TL;DR: To the authors' knowledge this is the first viable transgenic pig model with chromatin-associated eGFP allowing both cell tracking and the study of chromatin dynamics in a large animal model. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: July 27, 2019

2017 journal article

Improved Chondrogenic Potential and Proteomic Phenotype of Porcine Chondrocytes Grown in Optimized Culture Conditions

Cellular Reprogramming, 19(4), 232–244.

By: S. Koh n, M. Purser n, R. Wysk n & J. Piedrahita n

author keywords: chondrocytes; serum-free culture; low oxygen; pig; proteomics
MeSH headings : Animals; Cartilage / cytology; Cartilage / metabolism; Cell Culture Techniques / methods; Chondrocytes / cytology; Chondrocytes / metabolism; Chondrogenesis; Culture Media / chemistry; Extracellular Matrix Proteins / biosynthesis; Gene Expression Regulation; Swine
TL;DR: Low-SFM culture conditions resulted in improved cell growth rates, reduced levels of de-differentiation during expansion, greater ability to re-differentiate into cartilage on induction, and an improved proteomic profile that resembles that of in vivo cartilage. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: July 27, 2019

2017 journal article

Orientation changes in the cruciate ligaments of the knee during skeletal growth: A porcine model

Journal of Orthopaedic Research, 35(12), 2725–2732.

By: S. Cone n, S. Simpson n, J. Piedrahita n, L. Fordham*, J. Spang* & M. Fisher n

author keywords: anterior cruciate ligament; posterior cruciate ligament; adolescent; porcine; MRI
MeSH headings : Animals; Anterior Cruciate Ligament / diagnostic imaging; Anterior Cruciate Ligament / growth & development; Female; Magnetic Resonance Imaging; Posterior Cruciate Ligament / diagnostic imaging; Posterior Cruciate Ligament / growth & development; Swine
TL;DR: The age‐dependent properties of the ACL and PCL in the female pig mirrored results found in female human patients, suggesting that the porcine model may provide a pre‐clinical platform to study the cruciate ligaments during skeletal growth. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018

2016 journal article

1 GENERATION OF A STABLE TRANSGENIC SWINE MODEL FOR CELL TRACKING AND CHROMOSOME DYNAMIC STUDIES

Reproduction, Fertility and Development, 28(2), 130.

By: R. Sper n, S. Simpson n, X. Zhang n, B. Collins n & J. Piedrahita n

TL;DR: The first transgenic porcine H2B-GFP model via CRISPR-CAS9 mediated recombination and somatic cell nuclear transfer (SCNT) is developed, representing an improved platform for regenerative medicine and chromosome dynamic and cancer biology studies. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2016 journal article

27 AN IMPROVED LARGE ANIMAL MODEL FOR THE STUDY OF ADULT STEM CELLS

Reproduction, Fertility and Development, 28(2), 143.

By: S. Simpson n, L. Gonzalez n, J. Chung n, A. Blikslager n, S. Magness n & J. Piedrahita n

TL;DR: The generation of a porcine stem cell reporter line using the combination of transcription activator-like effector nucleases and somatic cell NT, which represents significant progress toward the study of adult stem cells, their progenitors, and the stem cell niche, using a large animal model with an anatomy, physiology, and ability to recapitulate human disease that overcomes the current limitations of rodent models. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 5, 2020

2016 journal article

Effect of Plant Species, Fertilizer Acidity/Basicity, and Fertilizer Concentration on pH of Soilless Root Substrate

HORTSCIENCE, 51(12), 1596–1601.

By: K. Jeong, P. Nelson, C. Niedziela & D. Dickey

author keywords: alkalinity; electrical conductivity; root media
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Cardiac regenerative potential of cardiosphere-derived cells from adult dog hearts

Journal of Cellular and Molecular Medicine, 19(8), 1805–1813.

By: M. Hensley n, J. Andrade n, B. Keene n, K. Meurs n, J. Tang n, Z. Wang*, T. Caranasos*, J. Piedrahita n ...

author keywords: cardiosphere-derived cells; dogs; dilated cardiomyopathy; stem cell therapy
MeSH headings : Aging; Animals; Apoptosis; Cell Differentiation; Cells, Cultured; Dogs; Female; Fibrosis; Heart / physiology; Heart Function Tests; Mice, SCID; Myocardium / pathology; Myocytes, Cardiac / cytology; Neovascularization, Physiologic; Paracrine Communication; Regeneration; Spheroids, Cellular / cytology
TL;DR: It is shown that canine CDCs can be produced from adult dog hearts and display similar phenotypes in comparison to previously studied CDCs derived from rodents and human beings. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2015 journal article

Characterization of putative haematopoietic cells from bovine yolk sac

Journal of Tissue Engineering and Regenerative Medicine, 11(4), 1132–1140.

By: V. Oliveira*, C. Mançanares*, L. Oliveira*, N. Gonçalves*, M. Miglino*, F. Perecin*, F. Meirelles*, J. Piedrahita n, C. Ambrósio*

author keywords: yolk sac; bovine; embryos; haematopoietic cell
MeSH headings : Animals; Cattle; Cell Adhesion / drug effects; Cell Survival / drug effects; Cells, Cultured; Colony-Forming Units Assay; Embryo, Mammalian / cytology; Female; Flow Cytometry; Hematopoietic Stem Cells / cytology; Hematopoietic Stem Cells / drug effects; Hematopoietic Stem Cells / ultrastructure; Methylcellulose / pharmacology; Polymerase Chain Reaction; Pregnancy; Yolk Sac / cytology
TL;DR: Cells were found to be capable of survival post‐cryopreservation and exhibited varying abilities to form colonies in a methylcellulose matrix, depending on gestational age. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: January 21, 2021

2015 chapter

Generation of Induced Pluripotent Stem Cells (iPSCs) from Adult Canine Fibroblasts

In Methods in Molecular Biology (pp. 69–78).

By: S. Koh* & J. Piedrahita n

MeSH headings : Animals; Cell Culture Techniques; Cell Differentiation; Cellular Reprogramming; Dogs; Feeder Cells; Fibroblasts / cytology; Fibroblasts / metabolism; Genetic Vectors / genetics; Induced Pluripotent Stem Cells / cytology; Induced Pluripotent Stem Cells / metabolism; Kruppel-Like Factor 4; Mice; Retroviridae / genetics; Transcription Factors / genetics; Transduction, Genetic; Transgenes
TL;DR: Detailed protocols for generation of iPSCs from adult canine fibroblasts are described, which will provide powerful tools not only to study human diseases, but also for the development of therapeutic approaches. (via Semantic Scholar)
Source: Crossref
Added: July 28, 2019

2015 journal article

Intravenous Cardiac Stem Cell-Derived Exosomes Ameliorate Cardiac Dysfunction in Doxorubicin Induced Dilated Cardiomyopathy

Stem Cells International, 2015, 1–8.

By: A. Vandergriff n, J. de Andrade n, J. Tang n, M. Hensley n, J. Piedrahita n, T. Caranasos*, K. Cheng n

TL;DR: Mice receiving CSC-XOs showed improved heart function via echocardiography, as well as decreased apoptosis and fibrosis, and mice showed no adverse immune reaction, showing promise for overcoming the limitations of stem cells and improving cardiac therapies. (via Semantic Scholar)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2014 journal article

5 DISRUPTION OF THE HIGH MOBILITY GROUP AT-HOOK 2 (HMGA2) GENE IN SWINE REDUCES POSTNATAL GROWTH

Reproduction, Fertility and Development, 26(1), 117.

By: J. Chung n, X. Zhang n, B. Colins n, K. Howard, S. Simpson n, C. Salmon, S. Koh n, R. Sper n, C. Byrd, J. Piedrahita n

TL;DR: The approach described here will result in a valuable large-animal model of dwarfism, but also in a tool to reduce the size of existing transgenic and nontransgenic swine lines, which will increase the receptivity of valuable transgenic lines by the biomedical community. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2014 review

From "ES-like" cells to induced pluripotent stem cells: A historical perspective in domestic animals

[Review of ]. THERIOGENOLOGY, 81(1), 103–111.

By: S. Koh n & J. Piedrahita n

author keywords: Pluripotent stem cells; Reprogramming; Large animals; Induced pluripotent stem cells
MeSH headings : Animals; Animals, Domestic / embryology; Animals, Domestic / physiology; Cattle; Dogs; Embryonic Stem Cells; Genomic Instability; Induced Pluripotent Stem Cells; Regenerative Medicine; Swine / physiology
TL;DR: A historical perspective of the isolation of various types of pluripotent stem cells in domesticated animals is provided and the latest progress and limitations in the derivation and application of iPSCs are summarized. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Stem Cells and Regenerative Medicine in Domestic and Companion Animals: A Multispecies Perspective

Reproduction in Domestic Animals, 49, 2–10.

By: N. Gonçalves, C. Ambrósio & J. Piedrahita n

MeSH headings : Adult; Animals; Animals, Domestic / embryology; Cats; Dogs; Embryonic Stem Cells; Horses; Humans; Induced Pluripotent Stem Cells; Mice; Pets / embryology; Rats; Regenerative Medicine / trends; Stem Cells
TL;DR: A range of stem cells are reviewed, from ESC to induced pluripotent stem cells, and their potential application in the field of regenerative medicine is discussed. (via Semantic Scholar)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2013 journal article

Alternating Current Electric Fields of Varying Frequencies: Effects on Proliferation and Differentiation of Porcine Neural Progenitor Cells

Cellular Reprogramming, 15(5), 405–412.

By: J. Lim n, S. McCullen n, J. Piedrahita n, E. Loboa n & N. Olby n

MeSH headings : Animals; Cell Differentiation; Cell Proliferation; Cells, Cultured; Electricity; Electrodes; Fluorescence; Glial Fibrillary Acidic Protein / metabolism; Neural Stem Cells / cytology; Swine; Swine, Miniature; Tubulin / metabolism
TL;DR: Higher frequency stimulation of low-frequency alternating current EFs via IDEs did not alter the alignment and proliferation of porcine neural progenitor cells, but higher frequency stimulation appeared to delay differentiation into mature astrocytes. (via Semantic Scholar)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2013 journal article

Cell lineage identification and stem cell culture in a porcine model for the study of intestinal epithelial regeneration

PLoS One, 8(6).

By: L. Gonzalez, I. Williamson, J. Piedrahita, A. Blikslager & S. Magness

Source: NC State University Libraries
Added: August 6, 2018

2013 journal article

Differences in X-Chromosome Transcriptional Activity and Cholesterol Metabolism between Placentae from Swine Breeds from Asian and Western Origins

PLOS ONE, 8(1).

By: S. Bischoff n, S. Tsai n, N. Hardison n, A. Motsinger-Reif n, B. Freking*, D. Nonneman*, G. Rohrer*, J. Piedrahita n

MeSH headings : Animals; Cholesterol / metabolism; Computational Biology; Female; Fertility / physiology; Gene Expression Profiling / veterinary; Gestational Age; Linear Models; Microarray Analysis / veterinary; Placenta / metabolism; Placenta / physiology; Pregnancy; RNA, Long Noncoding / genetics; Real-Time Polymerase Chain Reaction / veterinary; Reverse Transcriptase Polymerase Chain Reaction / veterinary; Species Specificity; Swine / genetics; Swine / physiology; Transcription, Genetic / physiology; X Chromosome / genetics; X Chromosome / metabolism
TL;DR: This work identifies key metabolic pathways that differ in the placentae of two swine breeds noted for differences in reproductive prolificacy. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

Differentially expressed microRNAs and affected biological pathways revealed by modulated modularity clustering (MMC) analysis of human preeclamptic and IUGR placentas

Placenta, 34(7), 599–605.

By: L. Guo n, S. Tsai*, N. Hardison n, A. James*, A. Motsinger-Reif n, B. Thames*, E. Stone n, C. Deng*, J. Piedrahita n

author keywords: Placenta; Preeclampsia; IUGR; MiR-194; MiR-149
MeSH headings : Down-Regulation; Female; Fetal Growth Retardation / genetics; Fetal Growth Retardation / metabolism; Humans; MicroRNAs / biosynthesis; Placenta / metabolism; Pre-Eclampsia / genetics; Pre-Eclampsia / metabolism; Pregnancy; Transcriptome
TL;DR: Modulated modularity clustering (MMC) allowed the elucidation of a molecular signature associated with preeclampsia and a subset of IUGR samples, which allowed the identification of genes, pathways, and microRNAs affected in these diseases. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
5. Gender Equality (Web of Science)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2013 journal article

Expression of antisense of insulin-like growth factor-2 receptor RNA non-coding (AIRN) during early gestation in cattle

Animal Reproduction Science, 138(1-2), 64–73.

By: W. Farmer n, P. Farin n, J. Piedrahita n, S. Bischoff n & C. Farin n

author keywords: Bovine; IGF2R; Antisense RNA; Embryo; Fetus
MeSH headings : Animals; Cattle / embryology; Embryo, Mammalian / metabolism; Female; Fertilization in Vitro / veterinary; Gene Expression Regulation, Developmental / genetics; Gene Expression Regulation, Developmental / physiology; Liver / metabolism; Male; Pregnancy; RNA, Messenger / chemistry; RNA, Messenger / genetics; RNA, Untranslated / genetics; Receptor, IGF Type 2 / genetics; Receptor, IGF Type 2 / metabolism; Reverse Transcriptase Polymerase Chain Reaction / veterinary
TL;DR: BAIRN was not expressed in blastocyst-stage embryos, was expressed in an increasing proportion of embryos around the time of maternal recognition of pregnancy and was expressed following implantation, indicating relative levels of bAIRN in bovine fetal liver can be altered by method of embryo production. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018

2013 journal article

Growth Requirements and Chromosomal Instability of Induced Pluripotent Stem Cells Generated from Adult Canine Fibroblasts

Stem Cells and Development, 22(6), 951–963.

By: S. Koh n, R. Thomas n, S. Tsai n, S. Bischoff n, J. Lim n, M. Breen n, N. Olby n, J. Piedrahita n

MeSH headings : Alkaline Phosphatase / metabolism; Animals; Antigens, Differentiation / genetics; Antigens, Differentiation / metabolism; Cell Differentiation; Cell Shape; Cells, Cultured; Chromosomal Instability; Coculture Techniques; Comparative Genomic Hybridization; Dogs; Fibroblasts / physiology; Gene Expression; In Situ Hybridization, Fluorescence; Induced Pluripotent Stem Cells / enzymology; Induced Pluripotent Stem Cells / pathology; Induced Pluripotent Stem Cells / transplantation; Kruppel-Like Factor 4; Mice; Mice, SCID; Recombinant Proteins / biosynthesis; Recombinant Proteins / genetics; Teratoma / genetics; Teratoma / pathology; Transcription Factors / biosynthesis; Transcription Factors / genetics
TL;DR: The isolated ciPSCs have similar characteristics to ESCs from other species, but the exact cellular mechanisms behind their unique co-dependency on both FGF2 and LIF are still unknown. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2013 journal article

Su1099 A Translational Porcine Model of Intestinal Stem Cells

Gastroenterology, 144(5), S-398.

By: L. Gonzalez*, A. Blikslager*, J. Piedrahita* & S. Magness

UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 5, 2020

2012 journal article

GENERATION AND CHARACTERISATION OF INDUCED PLURIPOTENT STEM CELLS (iPSCs) FROM ADULT CANINE FIBROBLASTS

Reproduction, Fertility and Development, 24(1), 285.

By: J. Piedrahita n, S. Koh n & N. Olby n

TL;DR: The isolated canine iPSCs have similar characteristics to ESCs from other species, but the exact cellular mechanisms behind their unique co-dependency on both FGF and LIF is still unknown. (via Semantic Scholar)
Source: Crossref
Added: February 24, 2020

2012 conference paper

Generation and characterization of induced pluripotent stem cells (iPS) from adult canine fibroblasts

Reproduction in Domestic Animals, 47, 418–419.

By: S. Koh, S. Tsai, S. Bischoff, R. Thomas, J. Lim, M. Breen, N. Olby, J. Piedrahita

Source: NC State University Libraries
Added: August 6, 2018

2012 journal article

Isolation and characterization of neural progenitor cells from adult canine brains

American Journal of Veterinary Research, 73(12), 1963–1968.

By: J. Lim n, S. Koh*, N. Olby*, J. Piedrahita* & C. Mariani*

MeSH headings : Animals; Cell Culture Techniques / veterinary; Cell Differentiation; Cell Lineage; Dogs; Female; Hippocampus / cytology; Hippocampus / metabolism; Immunohistochemistry / veterinary; Lateral Ventricles / cytology; Lateral Ventricles / metabolism; Male; Neural Stem Cells / cytology; Neural Stem Cells / physiology; RNA / analysis; Reverse Transcriptase Polymerase Chain Reaction
TL;DR: Results suggested the persistence of SOX2- Positive, nestin-positive, GFAP- positive,GFAP-Positive, OCT4-negative, and NANOG-negative neural progenitor cells in the SVZ and SGZ regions of mature canine brains, which are capable of producing multiple cell lineages. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries, ORCID
Added: August 6, 2018

2012 journal article

Lack of genomic imprinting of DNA primase, polypeptide 2 (PRIM2) in human term placenta and white blood cells

EPIGENETICS, 7(5), 429–431.

By: J. Chung n, S. Tsai n, A. James*, B. Thames*, S. Shytle* & J. Piedrahita n

author keywords: genomic imprinting; PRIM2; pseudogene; placenta; WBC; SNP; sequencing
MeSH headings : Alleles; DNA Primase / genetics; DNA Primase / metabolism; DNA Replication; Databases, Nucleic Acid; Female; Gene Frequency; Genetic Loci; Genome, Human; Genomic Imprinting; Heterozygote; Humans; Leukocytes / cytology; Leukocytes / enzymology; Molecular Sequence Annotation; Placenta / cytology; Placenta / enzymology; Polymorphism, Single Nucleotide; Pregnancy; Pseudogenes; RNA, Messenger / genetics; RNA, Messenger / metabolism; Transcription, Genetic
TL;DR: It is reported here their failure to confirm imprinting of the PRIM2 locus in human placenta or WBC, and the discordance between results and those of others is likely due to an incorrectly annotatedPRIM2 pseudogene found in the human genome database. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Transgenic Stra8-EYFP pigs: a model for developing male germ cell technologies

TRANSGENIC RESEARCH, 21(2), 383–392.

By: J. Sommer n, L. Jackson n, S. Simpson n, E. Collins n, J. Piedrahita n & R. Petters n

author keywords: Pig; Transgenic; Stra8; Spermatogonia; Marker gene
MeSH headings : Adaptor Proteins, Signal Transducing; Animals; Animals, Genetically Modified / genetics; Animals, Genetically Modified / metabolism; Blotting, Western; Cells, Cultured; Cloning, Molecular; Fibroblasts / cytology; Fibroblasts / drug effects; Fibroblasts / metabolism; Gene Expression Regulation, Developmental; Genes, Reporter; Germ Cells / cytology; Germ Cells / metabolism; Homeodomain Proteins / genetics; Homeodomain Proteins / metabolism; Immunohistochemistry; Luminescent Proteins / genetics; Luminescent Proteins / metabolism; Male; Mice; Microscopy, Fluorescence / methods; Mitochondria / genetics; Mitochondria / metabolism; Nuclear Transfer Techniques; Plasmids / genetics; Plasmids / metabolism; Promoter Regions, Genetic; Proteins / genetics; Proteins / metabolism; Swine; Testis / cytology; Testis / metabolism; Transfection; Transgenes; Tretinoin / pharmacology
TL;DR: Transgenic pigs expressing mitochondrial localized enhanced yellow fluorescent protein (EYFP-mito) under control of the germ cell specific Stimulated by Retinoic Acid 8 (Stra8) promoter indicates that these pigs will be useful by providing labelled cells for use in such technologies such as germ cell transplantation and in vitro spermatogenic studies. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

336 TRANSGENIC Stra8-EYFP PIGS: A MODEL FOR DEVELOPING MALE GERM CELL TECHNOLOGIES

Reproduction, Fertility and Development, 23(1), 264.

By: J. Sommer n, L. Jackson n, S. Simpson n, E. Collins n, J. Piedrahita n & R. Petters n

UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2011 conference paper

Perspectives on transgenic livestock in agriculture and biomedicine: An update

Reproduction, Fertility and Development, 23(1), 56–63.

By: J. Piedrahita & N. Olby

Source: NC State University Libraries
Added: August 6, 2018

2011 journal article

Perspectives on transgenic livestock in agriculture and biomedicine: an update

Reproduction, Fertility and Development, 23(1), 56.

By: J. Piedrahita n & N. Olby n

author keywords: animal models; bioreactors; somatic cell nuclear transfer; spinal cord repair; xenotransplantation
MeSH headings : Agriculture / trends; Animals; Animals, Domestic; Animals, Genetically Modified; Biomedical Technology / trends; Nuclear Transfer Techniques / trends; Nuclear Transfer Techniques / veterinary; Pluripotent Stem Cells; Stem Cell Research
TL;DR: The combination of advances in somatic cell nuclear transfer, the development of induced pluripotent stem cells and the completion of the sequencing of most livestock genomes ensures a bright and exciting future for this field, not only in livestock but also in companion animal species. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Crossref
Added: February 24, 2020

2011 journal article

Production of ELOVL4 transgenic pigs: a large animal model for Stargardt-like macular degeneration

British Journal of Ophthalmology, 95(12), 1749–1754.

By: J. Sommer n, J. Estrada n, E. Collins n, M. Bedell*, C. Alexander n, Z. Yang*, G. Hughes*, B. Mir n ...

MeSH headings : Animals; Animals, Genetically Modified; Disease Models, Animal; Electroretinography; Eye Proteins / biosynthesis; Eye Proteins / genetics; Gene Deletion; Immunohistochemistry; Macular Degeneration / metabolism; Macular Degeneration / pathology; Macular Degeneration / physiopathology; Membrane Proteins / biosynthesis; Membrane Proteins / genetics; Microscopy, Fluorescence; Mutation; Retina / metabolism; Retina / pathology; Retina / physiopathology; Swine
TL;DR: These transgenic pigs provide unique animal models for examining macular degeneration and STGD3 pathogenesis and direct fluorescence microscopy indicated that the Y270terEYFP protein is expressed in photoreceptors and mislocalised within the cell. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2011 review

The Role of Imprinted Genes in Fetal Growth Abnormalities

[Review of ]. BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY, 91(8), 682–692.

By: J. Piedrahita n

MeSH headings : Animals; Epigenesis, Genetic; Female; Fetal Development; Fetus / abnormalities; Gene Expression Regulation, Developmental; Genomic Imprinting / genetics; Humans; Pregnancy; Pregnancy Complications / genetics
TL;DR: Imprinting-associated disorders in humans and assisted reproductive technologies in animals have been shown to affect the epigenome of the early embryo and the expression of imprinted genes, and their role in disorders such as intrauterine growth restriction appears to be more complex. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Transcriptional profiling of human placentas from pregnancies complicated by preeclampsia reveals disregulation of sialic acid acetylesterase and immune signalling pathways

PLACENTA, 32(2), 175–182.

By: S. Tsai n, N. Hardison n, A. James*, A. Motsinger-Reif n, S. Bischoff n, B. Thames*, J. Piedrahita n

author keywords: Placenta; Preeclampsia; Maternal hypertension; Sialic acid acetylesterase
MeSH headings : Acetylesterase / biosynthesis; Antigens, CD / biosynthesis; Endoglin; Female; Genome-Wide Association Study; Humans; Infant, Newborn; Inhibins / biosynthesis; Male; Pre-Eclampsia / etiology; Pre-Eclampsia / metabolism; Pregnancy; Protein Array Analysis; Receptors, Cell Surface / biosynthesis; Trophoblasts / physiology; Up-Regulation; Vascular Endothelial Growth Factor Receptor-1 / biosynthesis
TL;DR: Evidence is found of preeclampsia-associated placental upregulation of sialic acid acetylesterase (SIAE), a gene functionally associated with autoimmune diseases, and changes in immune-associated signaling pathways, offering a potential upstream explanation for the shallow trophoblast invasion and inadequate uterine remodeling typically observed in pathogenesis of preeClampsia. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
5. Gender Equality (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Development of a Model of Sacrocaudal Spinal Cord Injury in Cloned Yucatan MiniPigs for Cellular Transplantation Research

CELLULAR REPROGRAMMING, 12(6), 689–697.

By: J. Lim n, J. Piedrahita n, L. Jackson n, T. Ghashghaei n & N. Olby n

MeSH headings : Animals; Behavior, Animal; Cauda Equina / pathology; Cauda Equina / surgery; Cell Culture Techniques; Cells, Cultured; Cloning, Organism; Green Fluorescent Proteins / metabolism; Humans; Models, Animal; Nerve Regeneration / physiology; Nuclear Transfer Techniques; Recovery of Function / physiology; Sacrum; Spinal Cord / pathology; Spinal Cord / surgery; Spinal Cord Injuries / pathology; Stem Cell Transplantation / methods; Swine; Swine, Miniature / surgery
TL;DR: It is demonstrated that transection of the sacrocaudal spinal cord in Yucatan SCNT clones produces profound, quantifiable neurological deficits restricted to the tail, which represents a powerful research tool to investigate the effect of cellular transplantation on axonal regeneration and functional recovery. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Generation and Characterization of Neurospheres from Canine Adipose Tissue-Derived Stromal Cells

CELLULAR REPROGRAMMING, 12(4), 417–425.

By: J. Lim n, L. Boozer n, C. Mariani n, J. Piedrahita n & N. Olby n

MeSH headings : Adipose Tissue / metabolism; Animals; Biomarkers / metabolism; Blotting, Western; Bone and Bones / cytology; Bone and Bones / metabolism; Cell Differentiation; Cell Lineage; Cells, Cultured; Dogs; Flow Cytometry; Neurons / metabolism; Osteogenesis; Phenotype; RNA, Messenger / genetics; Reverse Transcriptase Polymerase Chain Reaction; Stem Cells / cytology; Stromal Cells / metabolism
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2010 journal article

Varying phenotypes in swine versus murine transgenic models constitutively expressing the same human Sonic hedgehog transcriptional activator, K5-HGLI2ΔN

Transgenic Research, 19(5), 869–887.

By: A. McCalla-Martin n, X. Chen*, K. Linder n, J. Estrada n & J. Piedrahita n

author keywords: Basal cell carcinoma; Pig; Somatic cell nuclear transfer; Gli2; Hedgehog
MeSH headings : Animals; Animals, Genetically Modified / genetics; Animals, Suckling; Epidermis / metabolism; Epidermis / pathology; Female; Fibroblasts / metabolism; Genetic Predisposition to Disease; Hair Follicle / metabolism; Humans; Keratins / metabolism; Kruppel-Like Transcription Factors / genetics; Kruppel-Like Transcription Factors / physiology; Male; Mice; Nuclear Proteins / genetics; Nuclear Proteins / physiology; Phenotype; Pyoderma / etiology; Recombinant Fusion Proteins / physiology; Skin Diseases, Infectious / etiology; Skin Diseases, Infectious / pathology; Species Specificity; Sus scrofa; Swine / metabolism; Transgenes; Zinc Finger Protein Gli2
TL;DR: There is a need for a multiple species animal model approach in order to better understand the role of Gli2 in mammalian skin, as constitutive expression of the human hGli2ΔN in keratinocytes results in cutaneous changes that have not been reported in the K5-hGli-N murine model. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2009 journal article

Characterization of Conserved and Nonconserved Imprinted Genes in Swine

BIOLOGY OF REPRODUCTION, 81(5), 906–920.

By: S. Bischoff n, S. Tsai n, N. Hardison n, A. Motsinger-Reif n, B. Freking*, D. Nonneman*, G. Rohrer*, J. Piedrahita n

author keywords: assisted reproductive technology; comparative genomic imprinting; epigenetics; gene regulation; genomic imprinting; parthenogenesis; placenta; swine parthenote
MeSH headings : Animals; Female; Fetus / anatomy & histology; Gene Expression Profiling; Gene Expression Regulation, Developmental; Genomic Imprinting / genetics; Oligonucleotide Array Sequence Analysis; Organ Size; Parthenogenesis / genetics; Placenta / anatomy & histology; Polymorphism, Single Nucleotide; Pregnancy; Reverse Transcriptase Polymerase Chain Reaction; Staining and Labeling; Swine / genetics
TL;DR: To increase the understanding of imprinted genes in swine, a comprehensive analysis of this gene family was carried out using two complementary approaches: expression and phenotypic profiling of parthenogenetic fetuses, and analysis of imprinting by pyrosequencing. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

EVALUATION OF THE SERRATIA MARCESCENS NUCLEASE (NucA) AS A TRANSGENIC CELL ABLATION SYSTEM IN PORCINE

ANIMAL BIOTECHNOLOGY, 20(4), 177–185.

By: I. Caballero n & J. Piedrahita n

author keywords: Ablation; DTA; Fibroblasts; NucA; Porcine
MeSH headings : Animals; Cell Separation / methods; Cells, Cultured; Electroporation / methods; Endodeoxyribonucleases / analysis; Endodeoxyribonucleases / genetics; Endodeoxyribonucleases / metabolism; Endoribonucleases / analysis; Endoribonucleases / genetics; Endoribonucleases / metabolism; Fibroblasts / metabolism; Gene Expression; Gene Transfer Techniques; Rabbits; Serratia / enzymology; Swine
TL;DR: Gene expression analysis of surviving colonies indicated that survival is related to low or absent expression of the toxic genes, and results indicate that the NucA gene, while capable of mammalian cell ablation, is less efficient than DTA. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

2 DETECTION OF ANTISENSE TO Igf2r (AIR) RNA IN CATTLE

Reproduction, Fertility and Development, 20(1), 81.

By: W. Farmer, P. Farin, S. Bischoff, J. Alexander, J. Piedrahita* & C. Farin*

TL;DR: The antisense ncRNA, Air, does exist in cattle and its relative expression is greatest following implantation, suggesting that bAir may be involved in regulating imprinted expression of Igf2r in cattle. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2008 journal article

262: Molecular signatures of preeclampsia in human term placentas

American Journal of Obstetrics and Gynecology, 199(6), S84.

By: S. Tsai n, N. Hardison n, S. Bischoff n, B. Thames*, A. Jamessd*, A. Motsinger-Reif n, J. Piedrahita n

UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2008 journal article

A comparative study on the efficiency of two enucleation methods in pig somatic cell nuclear transfer: Effects of the squeezing and the aspiration methods

ANIMAL BIOTECHNOLOGY, 19(2), 71–79.

By: E. Lee n, J. Estrada* & J. Piedrahita*

author keywords: enucleation; in vivo viability; pig; somatic cell nuclear transfer
MeSH headings : Animals; Animals, Newborn; Biotechnology / methods; Embryo Transfer / veterinary; Female; Litter Size; Nuclear Transfer Techniques / veterinary; Oocytes; Pregnancy; Swine
TL;DR: The results indicate that the aspiration method for oocyte enucleation is more efficient than the squeezing method in producing a large number of pig SCNT embryos with normal in vivo viability. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

Enhancement of extra chromosomal recombination in somatic cells by affecting the ratio of homologous recombination (HR) to non-homologous end joining (NHEJ)

ANIMAL BIOTECHNOLOGY, 19(1), 6–21.

By: G. Zaunbrecher*, B. Mir*, P. Dunne n, M. Breen n & J. Piedrahita n

author keywords: extra chromosomal; homologous recombination; somatic cells
MeSH headings : Acetyltransferases / genetics; Animals; Cell Physiological Phenomena; Chromosomes / genetics; DNA Primers; DNA Repair; Plasmids; Recombination, Genetic; Swine; Tumor Suppressor Protein p53 / genetics
TL;DR: Results show that attempts at reducing NHEJ by use of Mre11 or anti-Ku aptamers were unsuccessful, and cell synchrony via thymidine incorporation does increase the ratio of HR/NHEJ and this indicates that this approach may be of use to facilitate targeting in somatic cells by reducing the numbers of colonies that need to be analyzed before a HR is identified. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

Identification of SNPs and INDELS in swine transcribed sequences using short oligonucleotide microarrays

BMC Genomics, 9(1), 252.

By: S. Bischoff n, S. Tsai n, N. Hardison n, A. York n, B. Freking*, D. Nonneman*, G. Rohrer*, J. Piedrahita n

MeSH headings : Animals; Base Sequence; Computational Biology; DNA / genetics; Female; Fetus / metabolism; Gene Expression Profiling; INDEL Mutation; Linear Models; Male; Oligonucleotide Array Sequence Analysis / methods; Oligonucleotide Array Sequence Analysis / statistics & numerical data; Placenta / metabolism; Polymorphism, Single Nucleotide; Pregnancy; RNA / genetics; Species Specificity; Swine / genetics
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2008 article

Successful cloning of the Yucatan minipig using commercial/occidental breeds as oocyte donors and embryo recipients

Estrada, J. L., Collins, B., York, A., Bischoff, S., Sommer, J., Tsai, S., … Piedrahita, J. A. (2008, June). CLONING AND STEM CELLS, Vol. 10, pp. 287–296.

By: J. Estrada n, B. Collins n, A. York n, S. Bischoff n, J. Sommer n, S. Tsai n, R. Petters n, J. Piedrahita n

MeSH headings : Animals; Cell Line; Cloning, Organism / methods; Fibroblasts / cytology; Microsatellite Repeats; Nuclear Transfer Techniques; Oocytes / physiology; Sus scrofa; Swine; Swine, Miniature / embryology
TL;DR: This report shows that it is possible to produce viable Yucatan SCNT clones and opens up the possibility of developing valuable biomedical models in this porcine breed. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Swine generated by somatic cell nuclear transfer have increased incidence of intrauterine growth restriction (IUGR)

CLONING AND STEM CELLS, 9(2), 229–236.

By: J. Estrada n, J. Sommer n, B. Collins n, B. Mir n, A. Martin n, A. York n, R. Petters n, J. Piedrahita n

MeSH headings : Animals; Birth Weight / physiology; Fetal Growth Retardation; Insemination, Artificial; Litter Size / physiology; Nuclear Transfer Techniques; Swine
TL;DR: SCNT-derived pigs are excellent models to study epigenetic factors and genes involved in IUGRs, and to develop effective means to improve fetal growth in humans and animals, and there are some differences between SCNT- derived and AI litters. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

263 USE OF PORCINE PARTHENOTES AND GENE EXPRESSION PROFILING USING MICROARRAYS FOR IDENTIFICATION OF IMPRINTED GENES

Reproduction, Fertility and Development, 18(2), 239.

By: J. Piedrahita*, S. Bischoff, J. Estrada, B. Freking, D. Nonneman, A. Martin, B. Mir, G. Rohrer, S. Tsai

TL;DR: Overall, utilizing the PRT models and gene expression profiles, thirteen genes where imprinting is conserved between swine and humans/mice, and several candidate genes that represent potentially imprinted genes are identified. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2006 journal article

32 CRYOPRESERVATION OF DONOR CELLS FOR NUCLEAR TRANSFER: EFFECT OF CELL FREEZING METHOD ON THE EFFICIENCY OF SOMATIC CELL NUCLEAR TRANSFER IN PIGS

Reproduction, Fertility and Development, 18(2), 125.

By: J. Estrada, E. Lee & J. Piedrahita*

TL;DR: Evaluated the effect of the cell freezing method on development of pig SCNT embryos in vitro and indicates that blastocyst development after SCNT does not differ when fetal fibroblasts are frozen by the two methods tested. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2006 article

Annotation of the Affymetrix(1) porcine genome microarray

Tsai, S., Cassady, J. P., Freking, B. A., Nonneman, D. J., Rohrer, G. A., & Piedrahita, J. A. (2006, August). ANIMAL GENETICS, Vol. 37, pp. 423–424.

By: S. Tsai n, J. Cassady n, B. Freking*, D. Nonneman*, G. Rohrer* & J. Piedrahita n

MeSH headings : Animals; DNA Probes; Gene Expression Profiling; Genomics; Humans; Oligonucleotide Array Sequence Analysis; Sequence Homology, Nucleic Acid; Swine / genetics
TL;DR: The strategy presented here is a strategy for improving the annotation of this microarray, which is minimally annotated on the basis of full-length porcine cDNA sequence. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

Dermatological effects of chronic exposure to 7,12-dimethylbenz[a]anthracene (DMBA) or N-methyl-N-nitrosoguanidine (MNNG) in swine

CUTANEOUS AND OCULAR TOXICOLOGY, 25(2), 103–119.

By: N. Monteiro-Riviere n, A. Inman n, V. Hedgpeth n, B. Mosteller n & J. Piedrahita n

Contributors: N. Monteiro-Riviere n, A. Inman n, V. Hedgpeth n, B. Mosteller n & J. Piedrahita n

author keywords: DMBA; MNNG; skin cancer; swine
MeSH headings : 9,10-Dimethyl-1,2-benzanthracene / toxicity; Analysis of Variance; Animals; Cocarcinogenesis; Female; Methylnitronitrosoguanidine / toxicity; Neoplasms, Radiation-Induced; Skin / drug effects; Skin / radiation effects; Skin Neoplasms / chemically induced; Swine; Ultraviolet Rays
TL;DR: The findings show that although chronic exposure to MNNG and DMBA, with or without UVB exposure, caused severe to mild dermatopathological changes, neither resulted in the development of skin carcinomas. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2006 journal article

Detection of transcriptional difference of porcine imprinted genes using different microarray platforms

BMC Genomics, 7, 328–336.

By: S. Tsai, B. Mir, A. Martin, J. Estrada, S. Bischoff, W. Hsieh, J. Cassady, B. Freking ...

Source: NC State University Libraries
Added: August 6, 2018

2006 article

Errors in development of fetuses and placentas from in vitro-produced bovine embryos

Farin, P. W., Piedrahita, J. A., & Farin, C. E. (2006, January 7). THERIOGENOLOGY, Vol. 65, pp. 178–191.

By: P. Farin n, J. Piedrahita n & C. Farin n

author keywords: bovine; embryo culture; nuclear transfer; fetus; placenta
MeSH headings : Animals; Breeding / methods; Cattle / embryology; Embryo Culture Techniques / methods; Embryo Culture Techniques / veterinary; Embryo Transfer / veterinary; Embryonic Development; Female; Fertilization in Vitro / veterinary; Gene Expression Regulation, Developmental; Male; Nuclear Transfer Techniques; Phenotype; Placenta / blood supply; Placenta / physiology; Placentation; Pregnancy; Pregnancy Complications / epidemiology; Pregnancy Complications / veterinary; Pregnancy Outcome / veterinary
TL;DR: The term 'abnormal offspring syndrome (AOS)' is introduced and a classification system of developmental outcomes is proposed to facilitate research efforts on the mechanisms of the various abnormal phenotypes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 journal article

51 PHENOTYPIC VARIATION IN CLONED SWINE IS CORRECTED IN THE F1 GENERATION

Reproduction, Fertility and Development, 17(2), 175.

By: B. Mir n, G. Zaunbrecher & J. Piedrahita n

TL;DR: The hypothesis that offspring of clones are to all intent and purposes indistinguishable from offspring of naturally bred animals, and as such there should not be any increased risks associated with consumption of products from these animals, is strongly supported. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2005 journal article

Developmental consequences of in utero sodium arsenate exposure in mice with folate transport deficiencies

TOXICOLOGY AND APPLIED PHARMACOLOGY, 203(1), 18–26.

By: O. Spiegelstein*, A. Gould*, B. Wlodarczyk*, M. Tsie*, X. Lu*, C. Le*, A. Troen*, J. Selhub* ...

author keywords: arsenic; teratogenicity; biotransformation; detoxification; Folbp1; RFC; neural tube defects
MeSH headings : Animals; Arsenates / toxicity; Biological Transport; Carrier Proteins / genetics; Carrier Proteins / metabolism; Chimera; Female; Folate Receptors, GPI-Anchored; Folic Acid / blood; Folic Acid / metabolism; Folic Acid Deficiency / embryology; Genotype; Male; Membrane Transport Proteins / deficiency; Membrane Transport Proteins / genetics; Membrane Transport Proteins / metabolism; Mice; Mice, Inbred C57BL; Mice, Knockout; No-Observed-Adverse-Effect Level; Receptors, Cell Surface / deficiency; Receptors, Cell Surface / genetics; Receptors, Cell Surface / metabolism; Reduced Folate Carrier Protein; Reproduction / drug effects
TL;DR: It is concluded that genetic modifications at the Folbp1 and RFC loci confers no particular sensitivity to arsenic toxicity compared to wild-type controls, thus disproving the working hypothesis that decreased methylating capacity of the genetically modified mice would put them at increased risk for arsenic-induced reproductive toxicity. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 journal article

Folate transport gene inactivation in mice increases sensitivity to colon carcinogenesis

Cancer Research, 65(3), 887–897.

By: D. Ma, R. Finnell, L. Davidson, E. Callaway, O. Spiegelstein, J. Piedrahita, J. Salbaum, C. Kappen ...

Source: NC State University Libraries
Added: August 6, 2018

2005 journal article

Progeny of somatic cell nuclear transfer (SCNT) pig clones are phenotypically similar to non-cloned pigs

CLONING AND STEM CELLS, 7(2), 119–125.

By: B. Mir n, G. Zaunbrecher*, G. Archer*, T. Friend* & J. Piedrahita n

MeSH headings : Animals; Body Weight; Cloning, Organism; Female; Litter Size; Male; Nuclear Transfer Techniques; Phenotype; Sex Ratio; Swine / genetics
TL;DR: The hypothesis that offspring of clones are similar to offspring of naturally bred animals, and as such there should not be any increased risks associated with consumption of products from these animals, is strongly supported. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 journal article

343NUCLEAR LOCALIZATION SIGNAL AND CELL SYNCHRONY ENHANCES GENE TARGETING EFFICIENCY IN FETAL BOVINE FIBROBLASTS

Reproduction, Fertility and Development, 16(2), 291.

By: B. Mir & J. Piedrahita*

TL;DR: Evidence is presented that the use of a nuclear localization signal (nls) and S-phase cell cycle synchronization by thymidine block enhances targeting efficiency at the hypoxanthine phosphoribosyl transferase locus in primary fetal bovine fibroblasts. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2004 journal article

35 Analysis of epigenetic modifications and genomic imprinting in nuclear transfer derived Bos Gaurus x B. Taurus Concepti

Reproduction, Fertility and Development, 16(2), 140.

By: S. Dindot*, P. Farin, C. Farin*, J. Alexander, E. Crosier, S. Walker*, C. Long*, J. Piedrahita*

TL;DR: The use of a bovine interspecies model for the assessment and characterization of epigenetic modifications and genomic imprinting in 40-day-old female nuclear transfer (NT)-derived fetuses and placentas and methylation analysis of the XIST Differentially Methylated Region indicates that cloning is associated with increased levels of methylation in selected genomic regions in the chorion. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: December 29, 2020

2004 journal article

67ORGAN WEIGHT VARIATION IN CLONED PIGS

Reproduction, Fertility and Development, 16(2), 155.

By: J. Piedrahita*, S. Walker* & G. Zaunbrecher*

TL;DR: Both absolute and corrected weights indicated that there is a high degree of variation in some of the organs among a group of identical clones, indicating that some organs in cloned animals have a high level of weight variability. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2004 journal article

Cloning and transgenesis in mammals: Implications for xenotransplantation

AMERICAN JOURNAL OF TRANSPLANTATION, 4, 43–50.

By: J. Piedrahita n & B. Mir n

MeSH headings : Animals; Animals, Genetically Modified; Cloning, Organism; Embryo, Mammalian / cytology; Graft Rejection / etiology; Graft Rejection / immunology; Graft Rejection / prevention & control; Humans; Immune Tolerance; Immunosuppression Therapy; Nuclear Transfer Techniques; Stem Cells / cytology; Swine; Transplantation, Heterologous / immunology; Transplantation, Heterologous / methods; Transplantation, Heterologous / trends
TL;DR: Advances in transgenic manipulation and cloning in swine are reviewed and how the development of these two technologies is critical to the eventual utilization of the pig as a human organ donor. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 journal article

Comparative PRNP genotyping of US cattle sires for potential association with BSE

MAMMALIAN GENOME, 15(10), 828–833.

By: C. Seabury*, J. Womack*, J. Piedrahita n & J. Derr*

MeSH headings : Alleles; Amyloid / genetics; Animals; Cattle / genetics; Encephalopathy, Bovine Spongiform / genetics; Genetic Variation; Genotype; Germany; Polymorphism, Single Nucleotide; Prions; Promoter Regions, Genetic / genetics; Protein Precursors / genetics; United States
TL;DR: Forward primer sequences from published primer sets targeting indels within the putative bovine PRNP promoter, intron 1, and the 3′ UTR were synthesized with unique 5′ fluorescent labels and utilized to develop a rapid multiplexed PCR assay for identifying BSE-associated indels as well as facilitating polymorphism analyses and/or marker-assisted selection. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 journal article

Conservation of genomic imprinting at the XIST, IGF2, and GTL2 loci in the bovine

Mammalian Genome, 15(12), 966–974.

By: S. Dindot*, K. Kent*, B. Evers*, N. Loskutoff, J. Womack* & J. Piedrahita n

MeSH headings : Alleles; Animals; Base Sequence; Cattle; DNA Methylation; Female; Gene Expression Regulation; Genomic Imprinting / genetics; Humans; Insulin-Like Growth Factor II / genetics; Male; Molecular Sequence Data; Placenta; Polymorphism, Single Nucleotide; Proteins / genetics; RNA, Long Noncoding; RNA, Untranslated / genetics; Sequence Homology, Nucleic Acid; Sheep
TL;DR: Conservation of genomic imprinting in the bovine is reported at the X chromosome inactivation–specific transcript (XIST), insulin-like growth factor 2 (IGF2), and gene trap locus 2 (GTL2) loci. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 journal article

Development of fetuses from in vitro produced and cloned bovine embryos

Journal of Animal Science, 82(Supplement 13), E53–E62.

By: C. Farin n, P. Farin n & J. Piedrahita n

MeSH headings : Animals; Cattle / embryology; Cattle / genetics; Cattle / growth & development; Cloning, Organism / adverse effects; Cloning, Organism / veterinary; Embryo Culture Techniques / veterinary; Embryo Transfer / adverse effects; Embryo Transfer / veterinary; Embryonic Development / genetics; Embryonic Development / physiology; Female; Fertilization in Vitro / adverse effects; Fertilization in Vitro / veterinary; Fetal Development / genetics; Fetal Development / physiology; Gene Expression Regulation, Developmental / physiology; Male; Pregnancy
TL;DR: The effects of in vitro production and cloning on bovine embryo and fetal development are examined, including abnormalities in the development of fetuses, placentas, and offspring following transfer of cloned embryos. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: October 29, 2021

2004 journal article

Epigenetic and genomic imprinting analysis in nuclear transfer derived Bos gaurus/Bos taurus hybrid fetuses

Biology of Reproduction, 71(2), 470–478.

By: S. Dindot n, P. Farin n, C. Farin n, J. Romano*, S. Walker*, C. Long, J. Piedrahita n

MeSH headings : Animals; Cattle; Chimera / genetics; Cloning, Organism; DNA Methylation; DNA, Satellite; Embryonic Development; Epidermis / physiology; Epigenesis, Genetic / genetics; Exons; Female; Fibroblasts / physiology; Genomic Imprinting / genetics; Genotype; Insulin-Like Growth Factor II / genetics; Keratins / genetics; Nuclear Transfer Techniques; Placenta; Pregnancy; Promoter Regions, Genetic; Proteins / genetics; RNA, Long Noncoding; RNA, Untranslated / genetics
TL;DR: The use of a bovine interspecies model for the assessment and characterization of epigenetic modifications and genomic imprinting in Day 40-old female NT-derived fetuses and placenta indicates that the nuclear transfer process affects gene expression patterns in the trophectoderm- and inner cell mass-derived tissues to different extents. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2004 journal article

Nuclear localization signal and cell synchrony enhance gene targeting efficiency in primary fetal fibroblasts

Nucleic Acids Research, 32(3), e25–e28.

By: B. Mir n & J. Piedrahita n

MeSH headings : Animals; Azaguanine / pharmacology; Base Sequence; Cattle; Cell Division / drug effects; Cell Division / physiology; Cells, Cultured; Drug Resistance; Fetus; Fibroblasts / cytology; Fibroblasts / drug effects; Fibroblasts / metabolism; Gene Targeting / methods; Genetic Vectors / genetics; Hypoxanthine Phosphoribosyltransferase / genetics; Male; Molecular Sequence Data; Mutagenesis, Insertional; Nuclear Localization Signals / genetics
TL;DR: It was determined that while no targeted colonies were identified using a conventional targeting construct, an average of 1 per million targeted cells were identified when a nuclear localization signal (nls) was added to the construct and targeting efficiency increased 7-fold. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2004 journal article

Somatic cell cloning: The ultimate form of nuclear reprogramming?

JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 15(5), 1140–1144.

By: J. Piedrahita*, B. Mir*, S. Dindot* & S. Walker*

MeSH headings : Animals; Cloning, Molecular / methods; Humans; Kidney Failure, Chronic / therapy; Stem Cell Transplantation / methods; Stem Cells / physiology
TL;DR: To make this a reality, techniques associated with cloning and genetic modifications in somatic cells need to be continued to be developed and optimized and have an effect beyond the area of nuclear transfer and assist with better methods for transdifferentiation of mammalian cells. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 journal article

Use of artificial androgen receptor coactivators to alter myoblast proliferation

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 91(3), 111–119.

By: C. Benjamin*, G. Jenster* & J. Piedrahita n

author keywords: androgen receptor; muscle growth; coactivators
MeSH headings : Animals; Base Sequence; Cell Division / drug effects; DNA Primers; HeLa Cells; Humans; Muscle, Skeletal / cytology; Muscle, Skeletal / drug effects; Mutagenesis, Site-Directed; Rats; Receptors, Androgen / drug effects; Receptors, Androgen / metabolism
TL;DR: The ability of the mutated AR to act as an artificial coactivator to up-regulate androgen responsive genes is a useful tool for understanding the interaction of androgens and muscle growth. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Behavioral variation among cloned pigs

Applied Animal Behaviour Science, 81(4), 321–331.

By: G. Archer*, T. Friend*, J. Piedrahita*, C. Nevill* & S. Walker*

author keywords: pig; swine; clone; behavior; variation; differences
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Behavioral variation among cloned pigs

Applied Animal Behaviour Science, 82(2), 151–161.

By: G. Archer*, T. Friend*, J. Piedrahita*, C. Nevill* & S. Walker*

TL;DR: Through a series of behavior tests, the variation in food preference, temperament, and time budgets of two genetically identical Duroc litters and their naturally bred controls are quantified. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: October 29, 2021

2003 journal article

Hierarchical phenotypic and epigenetic variation in cloned swine

BIOLOGY OF REPRODUCTION, 69(2), 430–436.

By: G. Archer*, S. Dindot*, T. Friend*, S. Walker*, G. Zaunbrecher*, B. Lawhorn*, J. Piedrahita*

author keywords: assisted reproductive technology; developmental biology; embryo; gene regulation
MeSH headings : Animals; Base Sequence; Centromere / genetics; Centromere / ultrastructure; Cloning, Molecular; Cloning, Organism / methods; CpG Islands / genetics; DNA Methylation; DNA, Satellite / genetics; Female; Genotype; Methylation; Mitochondria / metabolism; Molecular Sequence Data; Phenotype; Reference Values; Repetitive Sequences, Nucleic Acid; Reverse Transcriptase Polymerase Chain Reaction; Sulfites / pharmacology; Swine / genetics
TL;DR: Although cloning creates animals within the normal phenotypic range, it increases the variability associated with some traits, contrary to the expectation that cloning can be used to reduce the size of groups involved in animal experimentation and to reproduce an animal with a homogenous set of desired traits. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Kidney-specific activity of the bovine uromodulin promoter

TRANSGENIC RESEARCH, 12(2), 191–201.

By: H. Kim*, I. Song* & J. Piedrahita*

author keywords: bovine uromodulin promoter; kidney expression; transgenic mice
MeSH headings : Animals; Base Sequence; Cattle; Cloning, Molecular; DNA Primers; In Situ Hybridization; Kidney Tubules / metabolism; Mice; Mice, Transgenic; Mucoproteins / genetics; NIH 3T3 Cells; Promoter Regions, Genetic; Uromodulin
TL;DR: Transfection of luciferase reporter constructs fused to various proximal 5′-flanking regions of the bovine uromodulin gene markedly increased Luciferase activity in mouse renal epithelial cells but not in mesenchymal cells and that the most critical cis elements of the urommodulin gene are located within the 600 bp upstream region. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Mutational analysis of LoxP sites for efficient Cre-mediated insertion into genomic DNA

GENESIS, 36(3), 162–167.

By: J. Thomson*, E. Rucker* & J. Piedrahita n

author keywords: Cre recombinase; mutant loxP; insertion; lambda integrase
MeSH headings : Base Sequence; DNA Mutational Analysis; DNA Primers; Electroporation; Integrases / genetics; Molecular Sequence Data; Mutagenesis, Insertional / methods; Mutation / genetics; Plasmids / genetics; Viral Proteins / genetics
TL;DR: The Cre/loxP system has been used in transgenic models primarily to excise DNA flanked by loxP sites for gene deletion, but the insertion reaction is more difficult to control since the excision event is kinetically favored. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

UP1 extends life of primary porcine fetal fibroblasts in culture

CLONING AND STEM CELLS, 5(2), 143–148.

By: B. Mir n, N. Tanner*, B. Chowdhary* & J. Piedrahita n

MeSH headings : Animals; Antioxidants / pharmacology; Cell Survival / drug effects; Cells, Cultured; Chromosome Aberrations; Culture; Electroporation; Fetus / cytology; Fibroblasts / cytology; Fibroblasts / drug effects; Glutathione / pharmacology; Heterogeneous-Nuclear Ribonucleoprotein Group A-B / genetics; Heterogeneous-Nuclear Ribonucleoprotein Group A-B / metabolism; Hydroxylamines / pharmacology; Karyotyping; Swine / embryology
TL;DR: Effect of two anti-oxidants, glutathione and n-t-butyl hydroxylamine, and of the expression of UP1, a shortened derivative of heterogeneous nuclear riboprotein (hnRNP)A1, on the life extension of primary porcine fibroblasts in culture is reported. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2002 journal article

A highly efficient method for porcine cloning by nuclear transfer using in vitro-matured oocytes

Cloning and Stem Cells, 2, 105–112.

By: S. Walker*, T. Shin*, G. Zaunbrecher*, J. Romano*, G. Johnson*, F. Bazer*, J. Piedrahita*

MeSH headings : Animals; Cloning, Organism / methods; Culture Techniques; Embryo Transfer; Female; Microsatellite Repeats / genetics; Nuclear Transfer Techniques; Oocytes / cytology; Pregnancy; Sex Ratio; Swine / genetics
TL;DR: A simple nuclear transfer protocol, utilizing commercially available in vitro-matured oocytes, that results in greater than 5% overall cloning efficiency is reported, and should facilitate the broad utilization of porcine cloning technology in transgenic and nontransgenic applications. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2002 journal article

Effects of follicular size of cytoplast donor on the efficiency of cloning in cattle

MOLECULAR REPRODUCTION AND DEVELOPMENT, 61(3), 317–326.

By: J. Piedrahita*, D. Wells*, A. Miller*, J. Oliver*, M. Berg*, A. Peterson*, H. Tervit*

author keywords: embryo development; nuclear transfer; oocyte quality
MeSH headings : Animals; Blastocyst / cytology; Body Weight; Cattle; Cell Nucleus / physiology; Cloning, Organism / methods; Culture Techniques; Cytoplasm / physiology; Cytoplasm / transplantation; Embryo Transfer; Embryo, Mammalian / cytology; Embryo, Mammalian / embryology; Embryonic and Fetal Development; Female; Fertilization in Vitro; Microsatellite Repeats / genetics; Nuclear Transfer Techniques; Oocytes / cytology; Oocytes / growth & development; Oocytes / transplantation; Ovarian Follicle / anatomy & histology; Ovarian Follicle / cytology; Ovarian Follicle / transplantation; Pregnancy; Ultrasonography, Prenatal
TL;DR: Results indicate that cytoplasts from follicles of 1–3 and 6–12 mm in diameter are equally developmentally competent when used in a nuclear transfer procedure. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2002 journal article

Inhibition of apoptosis in serum starved porcine embryonic fibroblasts

MOLECULAR REPRODUCTION AND DEVELOPMENT, 62(1), 106–112.

By: C. Lee* & J. Piedrahita*

author keywords: cell synchronization; antioxidants; G(0)/G(1)
MeSH headings : Acetylcysteine / pharmacology; Animals; Antioxidants / pharmacology; Apoptosis; Cells, Cultured; Culture Media, Serum-Free; Female; Fibroblasts / cytology; Fibroblasts / drug effects; Free Radical Scavengers / pharmacology; Protease Inhibitors / pharmacology; Swine; alpha-Macroglobulins / pharmacology
TL;DR: The addition of MAC and antioxidants during serum starvation of PEF reduces apoptosis of quiescent fibroblasts and may contribute to increasing the efficiency of nuclear transplantation by improving the quality of donor nuclei. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2001 journal article

Inactivation of the whey acidic protein (WAP) gene by site-specific recombination in mouse embryonic stem cells

Journal of Animal Science, 42(6), 941–956.

By: C. Lee & J. Piedrahita

Source: NC State University Libraries
Added: August 6, 2018

2001 journal article

Stable transfection of the bovine NRAMP1 gene into murine RAW264.7 cells: effect on Brucella abortus survival

Infection and Immunity, 69(5), 3110–3119.

By: R. Barthel, J. Feng*, J. Piedrahita*, D. McMurray*, J. Templeton & L. Adams

MeSH headings : 3' Untranslated Regions; Animals; Base Sequence; Brucella abortus / growth & development; Carrier Proteins / genetics; Carrier Proteins / physiology; Cation Transport Proteins; Cattle; Cell Line; Interferon-gamma / pharmacology; Lipopolysaccharides / pharmacology; Macrophages / microbiology; Male; Membrane Proteins / genetics; Membrane Proteins / physiology; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Nitric Oxide / physiology; Transfection; Tumor Necrosis Factor-alpha / physiology
TL;DR: It is demonstrated that the 5′-flanking region of bovineNRAMP1, despite the lack of TATA and CAAT boxes, has a functional promoter capable of driving the expression of a transgene in murine macrophages. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2000 journal article

A rapid and sensitive reporter gene that uses green fluorescent protein expression to detect chemicals with estrogenic activity

TOXICOLOGICAL SCIENCES, 55(1), 69–77.

By: S. Miller*, D. Kennedy, J. Thomson, F. Han, R. Smith, N. Ing, J. Piedrahita*, D. Busbee

author keywords: cellular steroid receptors; endocrine disruption; xenobiotics; transfection; MCF7-ERE cells; estrogenic activity
MeSH headings : Cell Division / drug effects; Cell Line; Drug Evaluation, Preclinical; Estradiol / pharmacology; Estradiol Congeners / pharmacology; Eukaryotic Cells / drug effects; Genes, Reporter / drug effects; Genes, Reporter / genetics; Green Fluorescent Proteins; Humans; Luminescent Proteins / biosynthesis; Luminescent Proteins / genetics; Phosphoglycerate Kinase / metabolism; Plasmids / genetics; Receptors, Estrogen / drug effects; Receptors, Estrogen / genetics; Receptors, Estrogen / metabolism; Serum Albumin, Bovine / chemistry; Tamoxifen / analogs & derivatives; Tamoxifen / pharmacology; Transfection
TL;DR: The ERE-PGK-GFP reporter gene system is capable of rapid GFP expression in the presence of low concentrations of E2, and of quantifying estrogenicity of chemicals compared with a standard curve of the natural ligand, 17beta-estradiol. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2000 journal article

Effects of growth factors and feeder cells on porcine primordial germ cells in vitro

Cloning and Stem Cells, 2, 197–206.

By: C. Lee* & J. Piedrahita*

MeSH headings : Alkaline Phosphatase / metabolism; Animals; Animals, Genetically Modified; COS Cells; Cell Culture Techniques; Cell Differentiation; Cell Membrane / metabolism; Chlorocebus aethiops; Culture Media / pharmacology; Embryo Culture Techniques; Embryo, Mammalian / cytology; Fibroblast Growth Factor 2 / metabolism; Fibroblasts / metabolism; Germ Cells / cytology; Germ Cells / metabolism; Growth Substances; Interleukin-6 / metabolism; Leukemia Inhibitory Factor; Lewis X Antigen / metabolism; Stem Cell Factor / metabolism; Stem Cells / cytology; Swine
TL;DR: A combination of growth factors and proper feeder cells are critical for the establishment of undifferentiated porcine EG cells. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2000 journal article

Effects of protease inhibitors and antioxidants on in vitro survival of porcine primordial germ cells

BIOLOGY OF REPRODUCTION, 63(3), 887–897.

By: C. Lee*, R. Weaks*, G. Johnson*, F. Bazer* & J. Piedrahita*

author keywords: apoptosis; conceptus; cytokines; growth factors
MeSH headings : Acetylcysteine / administration & dosage; Acetylcysteine / pharmacology; Animals; Antioxidants / pharmacology; Apoptosis / drug effects; Cell Survival / drug effects; Cells, Cultured; DNA Fragmentation; Dose-Response Relationship, Drug; Fetus / cytology; Germ Cells / drug effects; Germ Cells / physiology; In Situ Nick-End Labeling; Microscopy, Electron; Protease Inhibitors / pharmacology; Swine; alpha-Macroglobulins / administration & dosage; alpha-Macroglobulins / pharmacology
TL;DR: Results indicate that the addition of α2-macroglobulin and antioxidants can increase the number of PGCs in vitro by suppressing apoptosis. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2000 journal article

Gene targeting in domestic species: A new beginning

Transgenic Res, 9(4/5), 261–262.

By: J. Piedrahita*

MeSH headings : Animals; Animals, Domestic / genetics; Gene Targeting / methods; Gene Targeting / veterinary
TL;DR: The prospects and technical challenges for introducing targeted changes into the germline by this route, and the types of application for which this new technology will be used, are discussed. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: October 29, 2021

2000 journal article

Isolation and genetic transformation of primordial germ cell (PGC)-derived cells from cattle, goats, rabbits and rats

ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 13(5), 587–594.

By: C. Lee, K. Moore, N. Scales, M. Westhusin, G. Newton, K. Im, J. Piedrahita*

author keywords: primordial germ cell; embryonic germ cell; alkaline phosphatase staining; embryoid body; genetic transformation; green fluorescent protein
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2000 journal article

Pathologic findings and association of Mycobacterium bovis infection with the bovine NRAMP1 gene in cattle from herds with naturally occurring tuberculosis

AMERICAN JOURNAL OF VETERINARY RESEARCH, 61(9), 1140–1144.

By: R. Barthel, J. Piedrahita*, D. McMurray, J. Payeur, D. Baca, F. Guemes, V. Perumaalla, T. Ficht, J. Templeton, L. Adams

MeSH headings : Alleles; Animals; Carrier Proteins / genetics; Cation Transport Proteins; Cattle / genetics; Genetic Predisposition to Disease / genetics; Genotype; Immunity, Innate / genetics; Membrane Proteins / genetics; Mycobacterium bovis / isolation & purification; Polymorphism, Restriction Fragment Length; Polymorphism, Single-Stranded Conformational; Tuberculosis, Bovine / genetics; Tuberculosis, Bovine / pathology
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2000 journal article

Three little pigs worth the huff and puff?

Nature Biotechnology, 18(11), 1144–1145.

By: M. Westhusin & J. Piedrahita*

MeSH headings : Animals; Cell Nucleus / metabolism; Cloning, Organism; Culture Techniques / methods; Ovum / metabolism; Swine; Transplantation, Heterologous
Sources: Crossref, NC State University Libraries
Added: July 27, 2019

1999 journal article

Inactivation of the folate binding protein genes disrupts neural tube closure.

Nature Genetics, 23, 228–232.

By: J. Piedrahita, B. Oetama, G. Bennett, J. Waes, S. Lacey, B. Kamen, J. Richardson, R. Lark, R. Finnell

Source: NC State University Libraries
Added: August 6, 2018

1999 journal article

Regulated Expression of MHC class I molecules in the porcine embryo

Biology of Reproduction, 60, 387–397.

By: J. Ramsoondar, R. Christopherson, L. Guilbert, W. Dixon, A. Ghahary, S. Ellis, T. Wegmann, J. Piedrahita

Source: NC State University Libraries
Added: August 6, 2018

1998 journal article

Cellular differentiation and I-FABP protein expression modulate fatty acid uptake and diffusion

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 274(3), C633–C644.

By: B. Atshaves, W. Foxworth, A. Frolov, J. Roths, A. Kier, B. Oetama, J. Piedrahita*, F. Schroeder

author keywords: embryonic stem cells; intracellular localization; laser cytometry; fluorescence recovery after photobleaching; intestinal fatty acid binding protein
MeSH headings : 4-Chloro-7-nitrobenzofurazan / analogs & derivatives; 4-Chloro-7-nitrobenzofurazan / pharmacokinetics; Animals; Carrier Proteins / biosynthesis; Carrier Proteins / genetics; Cell Differentiation; Cells, Cultured; Clone Cells / metabolism; Diffusion; Fatty Acid-Binding Protein 7; Fatty Acid-Binding Proteins; Fatty Acids / metabolism; Indicators and Reagents / pharmacokinetics; Mice; Myelin P2 Protein / biosynthesis; Myelin P2 Protein / genetics; Neoplasm Proteins; Nerve Tissue Proteins; Polymerase Chain Reaction; Restriction Mapping; Stearates / pharmacokinetics; Stem Cells / metabolism; Transfection
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1998 journal article

Factors affecting the efficiency of introducing precise genetic changes in ES cells by homologous recombination: tag-and-exchange versus the Cre-loxp system

TRANSGENIC RESEARCH, 7(3), 181–193.

By: J. Vazquez*, C. Nogues*, E. Rucker* & J. Piedrahita*

author keywords: gene targeting; homologous recombination; Cre-LoxP; site-specific recombination
MeSH headings : Animals; Apolipoproteins E / genetics; Cells, Cultured; Electroporation; Embryo, Mammalian / cytology; Gene Targeting; Genetic Markers; Genetic Vectors; Integrases / metabolism; Mice; Milk Proteins / genetics; Mutation; Plasmids; Recombination, Genetic; Selection, Genetic; Stem Cells; Thymidine Kinase / genetics; Viral Proteins
TL;DR: This work analysed how the distance between two gene markers placed within the region of homology of a targeting vector affects the rate at which both markers are introduced into the locus during the homologous recombination event. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1998 journal article

Generation of transgenic porcine chimeras using primordial germ cell-derived colonies

BIOLOGY OF REPRODUCTION, 58(5), 1321–1329.

By: J. Piedrahita*, K. Moore*, B. Oetama*, C. Lee*, N. Scales*, J. Ramsoondar*, F. Bazer*, T. Ott*

MeSH headings : Animals; Animals, Genetically Modified / genetics; Animals, Genetically Modified / physiology; Blotting, Southern; Cell Differentiation / physiology; Cells, Cultured; Chimera / genetics; Female; Germ Cells / physiology; Pregnancy; Swine; Transformation, Genetic
TL;DR: Results indicating that porcine EG cell lines can be isolated, genetically transformed, and utilized to make transgenic chimeras are presented. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1998 journal article

Isolation, characterization, and polymorphism detection of the porcine apolipoprotein E gene

Animal Genetics, 29, 43–47.

By: J. Ramsoondar*, J. Vazques, E. Rucker*, R. Dickson, J. Lunney & J. Piedrahita*

MeSH headings : Animals; Apolipoproteins E / chemistry; Apolipoproteins E / genetics; Apolipoproteins E / isolation & purification; Base Sequence; Chromosome Mapping / veterinary; Cloning, Molecular; DNA Primers; Disease Models, Animal; Exons / genetics; Gene Frequency; In Situ Hybridization, Fluorescence / veterinary; Introns / genetics; Microsatellite Repeats / genetics; Molecular Sequence Data; Protein Sorting Signals / genetics; Restriction Mapping / veterinary; Sequence Analysis, DNA / veterinary; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Swine / genetics
TL;DR: The present report describes the isolation and genetic characterization of the porcine apolipoprotein E (apo-E) gene, which contains a (CG)13 microsatellite marker within intron three that is moderately polymorphic. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1997 journal article

Cre-mediated site-directed insertions at the mouse whey acidic protein (mWAP) locus

Molecular Reproduction and Development, 48, 324–331.

By: E. Rucker* & J. Piedrahita*

Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1997 journal article

Effect of LIF and culture media on the in vitro differentiation of isolated porcine inner cell masses

In Vitro Cellular & Developmental Biology. Animal, 33, 61–70.

By: K. Moore & J. Piedrahita

Source: NC State University Libraries
Added: August 6, 2018

1997 journal article

Genetic characterization of the bovine leukaemia inhibitory factor (LIF) gene: Isolation and sequencing, chromosome assignment and microsatellite analysis

ANIMAL GENETICS, 28(1), 14–20.

By: J. Piedrahita*, R. Weaks*, A. Petrescu*, T. Shrode*, J. Derr* & J. Womack*

author keywords: bovine; chromosome 17; leukaemia inhibitory factor; LIF; mapping; microsatellite
MeSH headings : Amino Acid Sequence; Animals; Base Sequence; Cattle; Chromosome Mapping; Cloning, Molecular; Genes / genetics; Growth Inhibitors / genetics; Interleukin-6; Introns / genetics; Leukemia Inhibitory Factor; Lymphokines / genetics; Microsatellite Repeats / genetics; Molecular Sequence Data; Polymorphism, Restriction Fragment Length; Regulatory Sequences, Nucleic Acid / genetics; Sequence Analysis, DNA; Sequence Homology, Amino Acid
TL;DR: The bovine leukaemia inhibitory factor gene is conclusively assigned to chromosome 17, and allelic variants at this locus successfully detected in Bos javanicus, Bos guarus and Bison bison but not in Odocoileus virginianus. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1997 journal article

Isolation, characterization, and chromosome assignment of the porcine ciliary neurotrophic factor

Animal Genetics, 28, 354–357.

By: R. Weaks, J. Ramsoondar, D. Gallager, C. Nogues & J. Piedrahita

Source: NC State University Libraries
Added: August 6, 2018

1997 journal article

Progress on the generation of transgenic pigs via cultured cells

Journal of Reproduction & Fertility, 52, 245–254.

By: J. Piedrahita, K. Moore, C. Lee, R. Weaks, J. Ramsoondar, B. Oetama, J. Vasquez

Source: NC State University Libraries
Added: August 6, 2018

1997 journal article

Strain dependent alterations in the expression of folate pathway genes following teratogenic exposure to valproic acid in a mouse model.

American Journal of Medical Genetics. Part A, 70, 303–311.

By: R. Finnell*, B. Wlodarczyk*, J. Craig*, J. Piedrahita* & G. Bennett*

Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

1996 journal article

Effect of hematopoetic cytokines on in vitro differentiation of isolated porcine inner cell masses

Molecular Reproduction and Development, 45, 139–144.

By: K. Moore & J. Piedrahita

Source: NC State University Libraries
Added: August 6, 2018

1995 journal article

CHARACTERISTICS OF CLONED CEREBROVASCULAR ENDOTHELIAL-CELLS FOLLOWING INFECTION WITH THEILERS VIRUS .2. PERSISTENT INFECTION

JOURNAL OF NEUROIMMUNOLOGY, 62(2), 127–135.

By: B. Sapatino*, A. Petrescu*, B. Rosenbaum*, R. Smith*, J. Piedrahita* & C. Welsh

author keywords: CEREBROVASCULAR ENDOTHELIAL CELLS; THEILERS VIRUS; PERSISTENT INFECTION; MHC CLASS I; FLOW CYTOMETRY
MeSH headings : Animals; Antigens, Viral / analysis; Brain / blood supply; Brain / virology; Clone Cells; Endothelium, Vascular / immunology; Endothelium, Vascular / virology; Flow Cytometry; Histocompatibility Antigens Class I / analysis; Histocompatibility Antigens Class II / analysis; Interleukin-1 / genetics; Mice; Mice, Inbred BALB C; Mice, Inbred CBA; Poliomyelitis / immunology; Poliomyelitis / virology; Theilovirus / immunology; Theilovirus / isolation & purification
TL;DR: Cloned cerebrovascular endothelial cells (CVE) persistently infected with Theiler's virus (PI-CVE) have been established and characterized and demonstrated increased levels of mRNA for IL-1 beta when compared to uninfected CVE. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1992 journal article

GENERATION OF MICE CARRYING A MUTANT APOLIPOPROTEIN-E GENE INACTIVATED BY GENE TARGETING IN EMBRYONIC STEM-CELLS

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 89(10), 4471–4475.

By: J. Piedrahita*, S. Zhang*, . Hagaman*, P. Oliver* & N. Maeda*

author keywords: HOMOLOGOUS RECOMBINATION; ATHEROSCLEROSIS
MeSH headings : Animals; Apolipoproteins E / genetics; Base Sequence; Blotting, Southern; Cell Line; Chimera; DNA / genetics; DNA / isolation & purification; Embryo, Mammalian; Mice; Mice, Mutant Strains; Molecular Sequence Data; Oligodeoxyribonucleotides; Plasmids; Polymerase Chain Reaction / methods; Recombination, Genetic; Restriction Mapping; Stem Cells / physiology
TL;DR: The endogenous apolipoprotein E (apoE) gene is inactivated by using gene targeting in mouse embryonic stem (ES) cells by using two targeting plasmids, both containing a neomycin-resistance gene that replaces a part of the apoE gene and disrupts its structure. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1992 journal article

PRODUCTION OF CHIMERIC HAMSTERS BY AGGREGATION OF 8-CELL EMBRYOS

BIOLOGY OF REPRODUCTION, 47(3), 347–354.

By: J. Piedrahita*, L. Gillespie* & N. Maeda*

MeSH headings : Animals; Blastocyst / cytology; Cell Aggregation; Chimera; Cricetinae; Female; Hair Color / genetics; Infertility, Male / genetics; Infertility, Male / pathology; Male; Mesocricetus; Phenotype; Reproduction; Spermatozoa / pathology; Testis / pathology
TL;DR: It is demonstrated that hamster chimeras can be obtained by aggregation of 8-cell-stage embryos by examining the testes from the sterile chimera, which were devoid of germ cells. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1992 journal article

SPONTANEOUS HYPERCHOLESTEROLEMIA AND ARTERIAL LESIONS IN MICE LACKING APOLIPOPROTEIN-E

SCIENCE, 258(5081), 468–471.

By: S. Zhang*, R. Reddick*, J. Piedrahita* & N. Maeda*

MeSH headings : Animals; Apolipoproteins E / deficiency; Apolipoproteins E / genetics; Cholesterol / blood; Disease Models, Animal; Hypercholesterolemia / genetics; Hypercholesterolemia / pathology; Lipoproteins / metabolism; Mice; Mice, Mutant Strains; Mutagenesis, Insertional; Triglycerides / blood
TL;DR: Apolipoprotein E-deficient mice generated by gene targeting were used to test this hypothesis and to make a mouse model for spontaneous atherosclerosis, with severe yet viable phenotype that should make them valuable for investigating genetic and environmental factors that modify the atherogenic process. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1990 journal article

INFLUENCE OF FEEDER LAYER TYPE ON THE EFFICIENCY OF ISOLATION OF PORCINE EMBRYO-DERIVED CELL-LINES

THERIOGENOLOGY, 34(5), 865–877.

By: J. Piedrahita*, G. Anderson* & R. Bondurant*

TL;DR: It was found that embryo-derived cell lines could be isolated only from theSTO and the STO with BRL-conditioned medium treatments and none of the feeders tested increased the efficiency of isolation or the growth characteristics of embryo- derived cell lines over that of STO feeders. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1990 journal article

ON THE ISOLATION OF EMBRYONIC STEM-CELLS - COMPARATIVE BEHAVIOR OF MURINE, PORCINE AND OVINE EMBRYOS

THERIOGENOLOGY, 34(5), 879–901.

By: J. Piedrahita*, G. Anderson* & R. Bondurant*

TL;DR: The results of this study show that conditions which allow isolation of ES cells from murine embryos allow the isolation of porcine embryo-derived cell lines sharing some, but not all, the characteristics of murine ES cells. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1989 journal article

PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST THE H-Y-ANTIGEN

JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 15(3), 195–205.

By: P. Booman, L. Kruijt, M. Tieman, J. Piedrahita*, R. Veerhuis, P. Deboer, F. Ruch*

MeSH headings : Animals; Antibodies, Monoclonal / biosynthesis; Antibodies, Monoclonal / immunology; Cattle; Cells, Cultured; Female; H-Y Antigen / immunology; Hybridomas / immunology; Male; Mice; Rabbits; Sex Characteristics; Testis / immunology
TL;DR: Several monoclonal antibodies appeared to be positive in all assays tested, suggesting that the molecule conferring the H-Y antigenicity lacks species-specificity and appears to be identical for soluble and membrane-bound H- Y antigen. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1985 journal article

Investigation of sperm cytotoxicity as an indicator of ability of antisera to detect male-specific antigen on preimplantation mouse embryos

Journal of Reproduction & Fertility, 74, 637–644.

By: J. Piedrahita* & G. Anderson*

MeSH headings : Adenosine Triphosphate / analysis; Animals; Blastocyst / immunology; Cytotoxicity, Immunologic; Embryo Transfer; Fluorescent Antibody Technique; H-Y Antigen / analysis; H-Y Antigen / immunology; Immune Sera / immunology; Male; Mice; Mice, Inbred C57BL; Spermatozoa / immunology; Spleen / immunology
TL;DR: Although the sperm cytotoxicity test is useful for screening antisera for the study of H-Y antigen expression on preimplantation embryos, nonfluorescent embryos are more accurately classified as females than are fluorescent embryos as male. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

Employment

Updated: June 18th, 2018 14:17

2002 - present

North Carolina State University Raleigh, NC, US
Distinguished Professor Molecular Biomedical Sciences

1991 - 2002

Texas A&M University College Station College Station, TX, US
Faculty Veterinary Anatomy and Public Health

Education

Updated: June 18th, 2018 14:16

1989 - 1991

University of North Carolina NC, NC, US
Postdoctorate Pathology

1985 - 1989

University of California Davis Davis, CA, US
PhD ANSC

1982 - 1985

University of California Davis Davis, CA, US
MSC Animals Science

Funding History

Funding history based on the linked ORCID record. Updated: December 10th, 2019 20:19

grant September 1, 2017 - August 31, 2021
Immunodeficient Pigs for Stem Cell­-Based Regenerative Medicine
Office of the Director
grant April 1, 2015 - July 31, 2017
Housing and analysis facilities for miniature and transgenic swine: a biomedical resource
Office of the Director
grant September 1, 2014 - August 31, 2015
SWINE IN BIOMEDICAL RESEARCH CONFERENCE 2014 CHALLENGES AND OPPORTUNITIES
National Institute of Food and Agriculture
grant July 15, 2014 - June 30, 2017
Improved large animal model for the study of adult stem cells
Office of the Director
grant March 20, 2014 - March 19, 2015
Swine in Biomedical Research Conference 2014, Challenges and Opportunities
Office of the Director
grant April 1, 2012 - March 31, 2014
Maximizing the utilization of porcine animal models by development of a pygmy pig
Office of the Director
grant September 1, 2007 - August 31, 2010
Development of a Swine Model of Marfan Syndrome
National Institute of Arthritis and Musculoskeletal and Skin Diseases
grant May 1, 2006 - February 28, 2012
IMPRINTED GENES IN NORMAL AND ABNORMAL PLACENTAL/FETAL FUNCTION
National Institute of Child Health and Human Development
grant September 30, 2003 - September 29, 2004
Genomics approaches in comparative medicine
National Center for Advancing Translational Sciences
grant December 23-31, 1999
GENE TARGETING NONMURINE MAMMALS BY NUCLEAR TRANSFER
Fogarty International Center
grant April 5, 1998 - March 31, 2009
Center for Rural and Environmental Health
National Institute of Environmental Health Sciences
grant April 1, 1998 - September 30, 2002
SITE DIRECTED TRANSGENICS USING THE CRELOX SYSTEM
National Center for Advancing Translational Sciences
grant December 15, 1993 - March 31, 2015
Transgenesis and Xenotransplantation
National Heart Lung and Blood Institute
grant August 1, 1993 - May 31, 1998
DEVELOPMENT OF A MODEL FOR TAY-SACHS DISEASE
National Institute of Neurological Disorders and Stroke

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