James B. Hemphill

Works (8)

Updated: July 24th, 2023 21:17

2015 article

Conditional Control of Alternative Splicing through Light-Triggered Splice-Switching Oligonucleotides

Hemphill, J., Liu, Q., Uprety, R., Samanta, S., Tsang, M., Juliano, R. L., & Deiters, A. (2015, March 3). Journal of the American Chemical Society.

By: J. Hemphill n, Q. Liu n, R. Uprety n, S. Samanta *, M. Tsang *, R. Juliano*, A. Deiters n

MeSH headings : Alternative Splicing / radiation effects; Animals; HeLa Cells; Humans; Light; Oligonucleotides / chemistry; Oligonucleotides / genetics; Zebrafish
topics (OpenAlex): Photochromic and Fluorescence Chemistry; RNA Interference and Gene Delivery; DNA and Nucleic Acid Chemistry
TL;DR: This work addressed site-specific installation of light-removable nucleobase-caging groups as well as photocleavable backbone linkers into synthetic SSOs that enables optochemical OFF → ON and ON → OFF switching of their activity and thus precise control of alternative splicing. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2014 article

MicroRNA Targeting of CoREST Controls Polarization of Migrating Cortical Neurons

Volvert, M.-L., Prévot, P.-P., Close, P., Laguesse, S., Pirotte, S., Hemphill, J., … Nguyen, L. (2014, May 1). Cell Reports.

By: M. Volvert*, P. Prévot*, P. Close *, S. Laguesse *, S. Pirotte*, J. Hemphill n, F. Rogister *, N. Kruzy* ...

MeSH headings : Animals; Cell Movement / physiology; Cell Polarity / physiology; Cells, Cultured; Cerebral Cortex / cytology; Co-Repressor Proteins; Mice; Mice, Transgenic; MicroRNAs / metabolism; Nerve Tissue Proteins / metabolism; Neurons / cytology; Neurons / metabolism; Repressor Proteins / metabolism
topics (OpenAlex): MicroRNA in disease regulation; Circular RNAs in diseases; Neurogenesis and neuroplasticity mechanisms
TL;DR: This work describes how microRNAs (miRNAs) control neuronal polarization during radial migration and emphasizes key roles of some miRNAs that control radial migration during cerebral corticogenesis. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2014 article

Site-Specific Promoter Caging Enables Optochemical Gene Activation in Cells and Animals

Hemphill, J., Govan, J., Uprety, R., Tsang, M., & Deiters, A. (2014, April 27). Journal of the American Chemical Society.

By: J. Hemphill n, J. Govan n, R. Uprety  n, M. Tsang * & A. Deiters n

MeSH headings : Animals; Base Sequence; DNA, Circular / chemistry; DNA, Circular / genetics; Green Fluorescent Proteins / genetics; HEK293 Cells; Humans; Molecular Sequence Data; Photolysis; Plasmids / chemistry; Plasmids / genetics; Promoter Regions, Genetic; Protein Serine-Threonine Kinases / genetics; Transcriptional Activation; Tumor Suppressor Proteins; Ultraviolet Rays; Zebrafish
topics (OpenAlex): Photoreceptor and optogenetics research; Photochromic and Fluorescence Chemistry; Retinal Development and Disorders
TL;DR: Through the light-responsive modification of plasmid-based gene expression systems, optochemical activation of an exogenous fluorescent reporter gene in both tissue culture and a live animal model, as well as light-induced overexpression of an endogenous signaling protein are demonstrated. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2013 article

DNA Computation in Mammalian Cells: MicroRNA Logic Operations

Hemphill, J., & Deiters, A. (2013, June 24). Journal of the American Chemical Society.

By: J. Hemphill n & A. Deiters n

MeSH headings : Computers, Molecular; DNA / chemistry; DNA / metabolism; HeLa Cells; Humans; MicroRNAs / chemistry; MicroRNAs / metabolism; Photochemical Processes
topics (OpenAlex): Advanced biosensing and bioanalysis techniques; RNA Interference and Gene Delivery; DNA and Nucleic Acid Chemistry
TL;DR: Nucleic acid logic gates that are functional in a cellular environment and recognize endogenous inputs significantly expand the potential of DNA computation to monitor, image, and respond to cell-specific markers. (via Semantic Scholar)
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Added: August 6, 2018

2013 article

Genetically Encoded Light-Activated Transcription for Spatiotemporal Control of Gene Expression and Gene Silencing in Mammalian Cells

Hemphill, J., Chou, C., Chin, J. W., & Deiters, A. (2013, August 9). Journal of the American Chemical Society.

By: J. Hemphill n, C. Chou n, J. Chin * & A. Deiters n

MeSH headings : Activating Transcription Factors / genetics; Activating Transcription Factors / metabolism; DNA-Directed RNA Polymerases / genetics; DNA-Directed RNA Polymerases / metabolism; Gene Expression Regulation, Enzymologic / genetics; Gene Silencing; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; HEK293 Cells; Humans; Light; Luciferases / genetics; Luciferases / metabolism; Models, Molecular; Molecular Structure; Photochemical Processes; Protein Engineering; Viral Proteins / genetics; Viral Proteins / metabolism
topics (OpenAlex): Photochromic and Fluorescence Chemistry; bioluminescence and chemiluminescence research; CRISPR and Genetic Engineering
TL;DR: The photocaging of a synthetic gene network using unnatural amino acid mutagenesis in mammalian cells was demonstrated with an engineered bacteriophage RNA polymerase, demonstrating tight spatial and temporal control. (via Semantic Scholar)
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Source: NC State University Libraries
Added: August 6, 2018

2012 article

DNA Computation: A Photochemically Controlled AND Gate

Prokup, A., Hemphill, J., & Deiters, A. (2012, January 9). Journal of the American Chemical Society.

By: A. Prokup n, J. Hemphill n & A. Deiters n

MeSH headings : Base Sequence; Computers, Molecular; DNA / chemistry; Logic; Photochemistry
topics (OpenAlex): Advanced biosensing and bioanalysis techniques; DNA and Nucleic Acid Chemistry; Molecular Sensors and Ion Detection
TL;DR: A photochemically controlled AND gate was developed through the incorporation of caged thymidine nucleotides into a DNA-based logic gate, using light as the logic inputs, and both spatial control and temporal control were achieved. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2012 article

Regulation of Transcription through Light-Activation and Light-Deactivation of Triplex-Forming Oligonucleotides in Mammalian Cells

Govan, J. M., Uprety, R., Hemphill, J., Lively, M. O., & Deiters, A. (2012, April 27). ACS Chemical Biology.

By: J. Govan n, R. Uprety n, J. Hemphill n, M. Lively* & A. Deiters n

MeSH headings : DNA / chemistry; DNA / metabolism; HEK293 Cells; Humans; Nucleic Acid Conformation; Oligonucleotides / chemistry; Oligonucleotides / metabolism; Photic Stimulation / methods; Transcription, Genetic / physiology
topics (OpenAlex): Photochromic and Fluorescence Chemistry; DNA and Nucleic Acid Chemistry; Advanced biosensing and bioanalysis techniques
TL;DR: These are the first examples of light-regulated TFOs and their application in the photochemical activation and deactivation of gene expression and hairpin loop structures were found to significantly increase the efficacy of phosphodiester DNA-based T FOs in tissue culture. (via Semantic Scholar)
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Source: NC State University Libraries
Added: August 6, 2018

2012 article

Spatiotemporal control of microRNA function using light-activated antagomirs

Connelly, C. M., Uprety, R., Hemphill, J., & Deiters, A. (2012, September 3). Molecular BioSystems.

By: C. Connelly n, R. Uprety n, J. Hemphill n & A. Deiters n

MeSH headings : Antagomirs; Apoptosis / drug effects; Apoptosis / radiation effects; Cell Differentiation / drug effects; Cell Differentiation / radiation effects; Cell Line, Tumor; Cell Proliferation / drug effects; Cell Proliferation / radiation effects; Humans; Light; MicroRNAs / antagonists & inhibitors; MicroRNAs / metabolism; MicroRNAs / radiation effects; Oligonucleotides / chemistry; Oligonucleotides / pharmacology; Oligonucleotides / radiation effects; Photochemistry; Ultraviolet Rays
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Advanced biosensing and bioanalysis techniques; Luminescence and Fluorescent Materials
TL;DR: The presented approach enables the precise regulation of miRNA function and miRNA networks with unprecedented spatial and temporal resolution using UV irradiation and can be extended to any miRNA of interest. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

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