John Barron McArthur

Works (3)

Updated: July 5th, 2023 15:44

2017 journal article

Polyketide Bioderivatization Using the Promiscuous Acyltransferase KirCII

ACS Synthetic Biology, 6(3), 421–427.

By: E. Musiol-Kroll *, F. Zubeil*, T. Schafhauser*, T. Härtner*, A. Kulik *, J. McArthur n, I. Koryakina n, W. Wohlleben * ...

author keywords: antibiotic; kirromycin; polyketide synthase; trans-acyltransferase; engineering click chemistry
MeSH headings : Acyltransferases / metabolism; Anti-Bacterial Agents / metabolism; Bacteria / metabolism; Bacterial Proteins / metabolism; Polyketide Synthases / metabolism; Polyketides / metabolism; Pyridones / metabolism
TL;DR: This work exploits the promiscuity of KirCII to generate new kirromycins with allyl- and propargyl-side chains in vivo, and the latter were utilized as educts for further modification by "click" chemistry. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

2013 journal article

Promiscuity of a modular polyketide synthase towards natural and non-natural extender units

Organic & Biomolecular Chemistry, 11(27), 4449.

By: I. Koryakina n, J. McArthur n, M. Draelos n & G. Williams n 

MeSH headings : Acyl Coenzyme A / chemistry; Acyl Coenzyme A / metabolism; Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Coenzyme A Ligases / chemistry; Coenzyme A Ligases / metabolism; Polyketide Synthases / chemistry; Polyketide Synthases / metabolism; Protein Structure, Tertiary; Rhizobium / chemistry; Rhizobium / enzymology; Rhizobium / metabolism; Substrate Specificity
TL;DR: A panel of chemo-enzymatically synthesized acyl-CoA's was used to probe the promiscuity of a polyketide synthase, revealing that the KS is remarkably tolerant to a diverse array of extender units, while the AT likely discriminates betweenextender units that are native to the producing organism. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
10. Reduced Inequalities (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

2012 journal article

Poly Specific trans-Acyltransferase Machinery Revealed via Engineered Acyl-CoA Synthetases

ACS Chemical Biology, 8(1), 200–208.

By: I. Koryakina*, J. McArthur*, S. Randall, M. Draelos*, E. Musiol*, D. Muddiman* , T. Weber *, G. Williams* 

MeSH headings : Acyltransferases / chemistry; Acyltransferases / genetics; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Chromatography, Liquid; Coenzyme A Ligases / chemistry; Coenzyme A Ligases / genetics; Crystallography, X-Ray; Genetic Variation; Models, Molecular; Substrate Specificity
TL;DR: Results reveal that polyketide biosynthetic machinery might be more tolerant to non-natural substrates than previously established, and that mutant synthetases are valuable tools for probing the specificity of biosynthetics machinery. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

Citation Index includes data from a number of different sources. If you have questions about the sources of data in the Citation Index or need a set of data which is free to re-distribute, please contact us.

Certain data included herein are derived from the Web of Science© and InCites© (2025) of Clarivate Analytics. All rights reserved. You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.