The Solution Structures and Interaction of SinR and SinI: Elucidating the Mechanism of Action of the Master Regulator Switch for Biofilm Formation in Bacillus subtilis - Milton, Morgan E.; Draughn, G. Logan; Bobay, Benjamin G.; Stowe, Sean D.; Olson, Andrew L.; Feldmann, Erik A.; Thompson, Richele J.; Myers, Katherine H.; Santoro, Michael T.; Kearns, Daniel B.; Cavanagh, John (JOURNAL OF MOLECULAR BIOLOGY, 2020)
Keywords: Biofilms; Bacillus subtilis; Transcriptional regulation; NMR solution structure

Re-sensitizing Multidrug Resistant Bacteria to Antibiotics by Targeting Bacterial Response Regulators: Characterization and Comparison of Interactions between 2-Aminoimidazoles and the Response Regulators BfmR from Acinetobacter baumannii and QseB from Francisella spp. - Milton, Morgan E.; Minrovic, Bradley M.; Harris, Danni L.; Kang, Brian; Jung, David; Lewis, Caleb P.; Thompson, Richele J.; Melander, Roberta J.; Zeng, Daina; Melander, Christian; Cavanagh, John (FRONTIERS IN MOLECULAR BIOSCIENCES, 2018)
Keywords: biofilms; antibiotic resistance; two-component systems; response regulators; Acinetobacter baumannii; Francisella

The Structure of the Biofilm-controlling Response Regulator BfmR from Acinetobacter baumannii Reveals Details of Its DNA-binding Mechanism - Draughn, G. Logan; Milton, Morgan E.; Feldmann, Erik A.; Bobay, Benjamin G.; Roth, Braden M.; Olson, Andrew L.; Thompson, Richele J.; Actis, Luis A.; Davies, Christopher; Cavanagh, John (Journal of Molecular Biology, 2018)

Evaluation of a 2-aminoimidazole variant as adjuvant treatment for dermal bacterial infections - Draughn, G. L.; Allen, C. L.; Routh, P. A.; Stone, M. R.; Kirker, K. R.; Boegli, A.; Schuchman, R. M.; Linder, K. E.; Baynes, R. E.; James, G.; Melander, C.; Pollard, A.; et al. (Drug Design Development and Therapy, 2017)

Potentiation of Francisella resistance to conventional antibiotics through small molecule adjuvants - Stephens, Matthew D.; Hubble, Veroncia B.; Ernst, Robert K.; Hoek, Monique L.; Melander, Roberta J.; Cavanagh, John; Melander, Christian (MEDCHEMCOMM, 2016)

Augmenting anti-cancer natural products with a small molecule adjuvant - Wahome, P. G.; Beauchesne, K. R.; Pedone, A. C.; Cavanagh, J.; Melander, C.; Zimba, P.; Moeller, P. D. R. (Marine Drugs, 2015)

Membrane-Permeabilizing Activity of Reverse-Amide 2-Aminoimidazole Antibiofilm Agents Against Acinetobacter baumannii - Stowe, Sean D.; Thompson, Richele J.; Peng, Lingling; Su, Zhaoming; Blackledge, Meghan S.; Draughn, G. Logan; Coe, William H.; Johannes, Eva; Lapham, Valerie K.; Mackenzie, John; Melander, Christian; Cavanagh, John (CURRENT DRUG DELIVERY, 2015)
Keywords: 2-aminoimidazole; Acinetobacter baumannii; biofilm; membrane permeabilization; reverse amide; SEM

Teaching old drugs new tricks: Addressing resistance in Francisella - Feldmann, Erik A.; Cavanagh, John (VIRULENCE, 2015)

A DNA Mimic: The Structure and Mechanism of Action for the Anti-Repressor Protein AbbA - Tucker, Ashley T.; Bobay, Benjamin G.; Banse, Allison V.; Olson, Andrew L.; Soderblom, Erik J.; Moseley, M. Arthur; Thompson, Richele J.; Varney, Kristen M.; Losick, Richard; Cavanagh, John (JOURNAL OF MOLECULAR BIOLOGY, 2014)
Keywords: transition state regulator; AbbA; DNA mimic; molecular docking; NMR

Chemical shift assignments and secondary structure prediction of the C-terminal domain of the response regulator BfmR from Acinetobacter baumannii - Olson, Andrew L.; Thompson, Richele J.; Melander, Christian; Cavanagh, John (BIOMOLECULAR NMR ASSIGNMENTS, 2014)
Keywords: NMR; BfmR; Acinetobacter baumannii; Assignment

Chemical shift assignments and secondary structure prediction of the master biofilm regulator, SinR, from Bacillus subtilis - Stowe, Sean D.; Olson, Andrew L.; Losick, Richard; Cavanagh, John (BIOMOLECULAR NMR ASSIGNMENTS, 2014)
Keywords: NMR; SinR; Bacillus subtilis; Biofilm regulation

Chemical shift assignments and secondary structure prediction of the phosphorelay protein VanU from Vibrio anguillarum - Bobay, Benjamin G.; Thompson, Richele J.; Milton, Debra L.; Cavanagh, John (BIOMOLECULAR NMR ASSIGNMENTS, 2014)
Keywords: VanU; NMR; Vibrio anguillarum; Phosphorelay

Small-Molecule Suppression of beta-Lactam Resistance in Multidrug-Resistant Gram-Negative Pathogens - Brackett, Christopher M.; Melander, Roberta J.; An, Il Hwan; Krishnamurthy, Aparna; Thompson, Richele J.; Cavanagh, John; Melander, Christian (JOURNAL OF MEDICINAL CHEMISTRY, 2014)

Structure and DNA-Binding Traits of the Transition State Regulator AbrB - Olson, Andrew L.; Tucker, Ashley T.; Bobay, Benjamin G.; Soderblom, Erik J.; Moseley, M. Arthur; Thompson, Richele J.; Cavanagh, John (STRUCTURE, 2014)

Chemical crosslinking and LC/MS analysis to determine protein domain orientation: Application to AbrB - Olson, Andrew L.; Liu, Fan; Tucker, Ashley T.; Goshe, Michael B.; Cavanagh, John (BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2013)
Keywords: NMR; Solution structure; Chemical crosslinking; Domain orientation; AbrB; Gas-phase cleavable

Evaluation of the toxicity of 2-aminoimidazole antibiofilm agents using both cellular and model organism systems - Stowe, Sean D.; Tucker, Ashley T.; Thompson, Richele; Piper, Amanda; Richards, Justin J.; Rogers, Steven A.; Mathies, Laura D.; Melander, Christian; Cavanagh, John (DRUG AND CHEMICAL TOXICOLOGY, 2012)
Keywords: 2-aminoimidazole; adjuvant; biofilm; Caenorhabditis elegans; toxicity; Toxicity of 2-aminoimidazole; antibiofilm agents

H-1, C-13, and N-15 resonance assignments and secondary structure prediction of the full-length transition state regulator AbrB from Bacillus anthracis - Olson, Andrew L.; Bobay, Benjamin G.; Melander, Christian; Cavanagh, John (BIOMOLECULAR NMR ASSIGNMENTS, 2012)
Keywords: AbrB; NMR; Bacillus anthracis; Transition state regulator

Identification of BfmR, a Response Regulator Involved in Biofilm Development, as a Target for a 2-Aminoimidazole-Based Antibiofilm Agent - Thompson, Richele J.; Bobay, Benjamin G.; Stowe, Sean D.; Olson, Andrew L.; Peng, Lingling; Su, Zhaoming; Actis, Luis A.; Melander, Christian; Cavanagh, John (BIOCHEMISTRY, 2012)

Structural insights into the calcium-dependent interaction between calbindin-D28K and caspase-3 - Bobay, Benjamin G.; Stewart, Amanda L.; Tucker, Ashley T.; Thompson, Richele J.; Varney, Kristen M.; Cavanagh, John (FEBS LETTERS, 2012)
Keywords: Calbindin-D28K; Caspase-3; Isothermal titration calorimetry; Molecular docking

Anti-biofilm compounds derived from marine sponges - Stowe, S. D.; Richards, J. J.; Tucker, A. T.; Thompson, R.; Melander, C.; Cavanagh, J. (Marine Drugs, 2011)

Flustramine inspired synthesis and biological evaluation of pyrroloindoline triazole amides as novel inhibitors of bacterial biofilms - Bunders, Cynthia; Cavanagh, John; Melander, Christian (ORGANIC & BIOMOLECULAR CHEMISTRY, 2011)

Intercepting Bacterial Indole Signaling with Flustramine Derivatives - Bunders, Cynthia A.; Minvielle, Marine J.; Worthington, Roberta J.; Ortiz, Minoshka; Cavanagh, John; Melander, Christian (JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2011)

Observing selected domains in multi-domain proteins via sortase-mediated ligation and NMR spectroscopy - Refaei, Mary Anne; Combs, Al; Kojetin, Douglas J.; Cavanagh, John; Caperelli, Carol; Rance, Mark; Sapitro, Jennifer; Tsang, Pearl (JOURNAL OF BIOMOLECULAR NMR, 2011)
Keywords: Segmental labeling; Sortase A; Protein ligation; MecA; Enzyme-mediated ligation; NMR

Binding site on human immunoglobulin G for the affinity ligand HWRGWV - Yang, H. O.; Gurgel, P. V.; Williams, D. K.; Bobay, B. G.; Cavanagh, J.; Muddiman, D. C.; Carbonell, R. G. (Journal of Molecular Recognition, 2010)

Long range dynamic effects of point-mutations trap a response regulator in an active conformation - Bobay, Benjamin G.; Thompson, Richele J.; Hoch, James A.; Cavanagh, John (FEBS LETTERS, 2010)
Keywords: NMR; Conformational transition; Intra-protein communication; Response regulator; Spo0F

NMR Solution Structure and DNA-binding Model of the DNA-binding Domain of Competence Protein A - Hobbs, Carey A.; Bobay, Benjamin G.; Thompson, Richele J.; Perego, Marta; Cavanagh, John (JOURNAL OF MOLECULAR BIOLOGY, 2010)
Keywords: competence; ComA; NMR; two-component; response regulator

Synergistic Effects between Conventional Antibiotics and 2-Aminoimidazole-Derived Antibiofilm Agents - Rogers, Steven A.; Huigens, Robert W., III; Cavanagh, John; Melander, Christian (ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2010)

Amide Isosteres of Oroidin: Assessment of Antibiofilm Activity and C. elegans Toxicity - Richards, Justin J.; Reyes, Samuel; Stowe, Sean D.; Tucker, Ashley T.; Ballard, T. Eric; Mathies, Laura D.; Cavanagh, John; Melander, Christian (JOURNAL OF MEDICINAL CHEMISTRY, 2009)

Binding site on human immunoglobulin G for the affinity ligand HWRGWV - Yang, Haiou; Gurgel, Patrick V.; Williams, D. Keith, Jr; Bobay, Benjamin G.; Cavanagh, John; Muddiman, David C.; Carbonell, Ruben G. (Journal of Molecular Recognition, 2009)
Keywords: hexamer ligand; immunoglobulin G; glycosylation; mass spectrometry; computational docking; binding site

Evaluation of dihydrooroidin as an antifouling additive in marine paint - Melander, Christian; Moeller, Peter D. R.; Ballard, T. Eric; Richards, Justin J.; Huigens, Robert W., III; Cavanagh, John (INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 2009)
Keywords: Antifouling; Biofilms; Natural products

Structural Characterization of the Conformational Change in Calbindin-D-28k upon Calcium Binding Using Differential Surface Modification Analyzed by Mass Spectrometry - Hobbs, Carey A.; Deterding, Leesa J.; Perera, Lalith; Bobay, Benjamin G.; Thompson, Richele J.; Darden, Thomas A.; Cavanagh, John; Tomer, Kenneth B. (BIOCHEMISTRY, 2009)

Structural and Motional Contributions of the Bacillus subtilis ClpC N-Domain to Adaptor Protein Interactions - Kojetin, Douglas J.; McLaughlin, Patrick D.; Thompson, Richele J.; Dubnau, David; Prepiak, Peter; Rance, Mark; Cavanagh, John (JOURNAL OF MOLECULAR BIOLOGY, 2009)
Keywords: competence; adaptor protein proteosome interactions; HSP100/Clp N-domain; NMR dynamics

Co-Evolving Motions at Protein−Protein Interfaces of Two-Component Signaling Systems Identified by Covariance Analysis† - Szurmant, Hendrik; Bobay, Benjamin G.; White, Robert A.; Sullivan, Daniel M.; Thompson, Richele J.; Hwa, Terence; Hoch, James A.; Cavanagh, John (Biochemistry, 2008)

Control of bacterial biofilms with marine alkaloid derivatives - Huigens, Robert W., III; Ma, Luyan; Gambino, Christopher; Moeller, Peter D. R.; Basso, Anne; Cavanagh, John; Wozniak, Daniel J.; Melander, Christian (MOLECULAR BIOSYSTEMS, 2008)

Inhibition and dispersion of proteobacterial biofilms - Richards, Justin J.; Huigens III, Robert W.; Ballard, T. Eric; Basso, Anne; Cavanagh, John; Melander, Christian (Chemical Communications, 2008)

Insights into the Nature of DNA Binding of AbrB-like Transcription Factors - Sullivan, Daniel M.; Bobay, Benjamin G.; Kojetin, Douglas J.; Thompson, Richele J.; Rance, Mark; Strauch, Mark A.; Cavanagh, John (STRUCTURE, 2008)

Targeting RNA with cysteine-constrained peptides - Burns, Virginia A.; Bobay, Benjamin G.; Basso, Anne; Cavanagh, John; Melander, Christian (BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2008)
Keywords: RNA; cyclic peptides; HADDOCK

Abh and AbrB control of Bacillus subtilis antimicrobial gene expression - Strauch, Mark A.; Bobay, Benjamin G.; Cavanagh, John; Yao, Fude; Wilson, Angelo; Le Breton, Yoann (JOURNAL OF BACTERIOLOGY, 2007)

NMR assignment of the N-terminal repeat domain of Bacillus subtilis ClpC - Kojetin, Douglas J.; McLaughlin, Patrick D.; Thompson, Richele J.; Venters, Ronald A.; Rance, Mark; Cavanagh, John (BIOMOLECULAR NMR ASSIGNMENTS, 2007)
Keywords: ClpC; Bacillus subtilis; N-domain; genetic competence; HSP100/AAA+

Peptide binding proclivities of calcium loaded calbindin-D28k - Kordys, David R.; Bobay, Benjamin G.; Thompson, Richele J.; Venters, Ronald A.; Cavanagh, John (FEBS LETTERS, 2007)
Keywords: calbindin-D28k; NMR; chemical shift; peptides; modeling

Predominantly buried residues in the response regulator Spo0F influence specific sensor kinase recognition - McLaughlin, Patrick D.; Bobaya, Benjamm G.; Regel, Erin J.; Thompson, Richele J.; Hoch, James A.; Cavanagh, John (FEBS LETTERS, 2007)
Keywords: sporulation; kinases; Spo0F mutants

Tandem mass spectrometry acquisition approaches to enhance identification of protein-protein interactions using low-energy collision-induced dissociative chemical crosslinking reagents - Soderblom, Erik J.; Bobay, Benjamin G.; Cavanagh, John; Goshe, Michael B. (RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2007)

Measurement of multisite oxidation kinetics reveals an active site conformational change in Spo0F as a result of protein oxidation - Sharp, JS; Sullivan, DM; Cavanagh, J; Tomer, KB (BIOCHEMISTRY, 2006)

NMR structure of AbhN and comparison with AbrBN - First insights into the DNA binding promiscuity and specificity of AbrB-like transition state regulator proteins - Bobay, Benjamin G.; Mueller, Geoffrey A.; Thompson, Richele J.; Murzin, Alexey G.; Venters, Ronald A.; Strauch, Mark A.; Cavanagh, John (JOURNAL OF BIOLOGICAL CHEMISTRY, 2006)

Structure, binding interface and hydrophobic transitions of Ca2+-loaded calbindin-D-28K - Kojetin, D. J.; Venters, R. A.; Kordys, D. R.; Thompson, R. J.; Kumar, R.; Cavanagh, J. (Nature Structural & Molecular Biology, 2006)

Structure, binding interface and hydrophobic transitions of Ca2+-loaded calbindin-D28K - Kojetin, Douglas J; Venters, Ronald A; Kordys, David R; Thompson, Richele J; Kumar, Rajiv; Cavanagh, John (Nature Structural & Molecular Biology, 2006)

(4,2)D projection-reconstruction experiments for protein backbone assignment: Application to human carbonic anhydrase II and calbindin D-28K - Venters, RA; Coggins, BE; Kojetin, D; Cavanagh, J; Zhou, P (JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005)

Revised structure of the AbrB N-terminal domain unifies a diverse superfamily of putative DNA-binding proteins - Bobay, BG; Andreeva, A; Mueller, GA; Cavanagh, J; Murzin, AG (FEBS LETTERS, 2005)
Keywords: NMR solution structure; protein-DNA interactions; structural genomics; structural classification of proteins

Solution structure and dynamics of LuxU from Vibrio harveyi, a phosphotransferase protein involved in bacterial quorum sensing - Ulrich, DL; Kojetin, D; Bassler, BL; Cavanagh, J; Loria, JP (JOURNAL OF MOLECULAR BIOLOGY, 2005)
Keywords: quorum-sensing; NMR; solution structure; LuxU; bioluminescence

Structural analysis of divalent metals binding to the Bacillus subtilis response regulator Spo0F: the possibility for In vitro metalloregulation in the initiation of sporulation - Kojetin, DJ; Thompson, RJ; Benson, LM; Naylor, S; Waterman, J; Davies, KG; Opperman, CH; Stephenson, K; Hoch, JA; Cavanagh, J (BIOMETALS, 2005)
Keywords: metal binding; NMR spectroscopy; response regulator; sporulation; two-component signal transduction

Evaluation of the DNA Binding Tendencies of the Transition State Regulator AbrB† - Bobay, Benjamin G.; Benson, Linda; Naylor, Stephen; Feeney, Brett; Clark, A. Clay; Goshe, Michael B.; Strauch, Mark A.; Thompson, Richele; Cavanagh, John (Biochemistry, 2004)

H-1, N-15, and C-13 chemical shift assignments of the Vibrio harveyi histidine phosphotransferase protein LuxU - Ulrich, DL; Thompson, R; Bassler, B; Cavanagh, J; Loria, JP (JOURNAL OF BIOMOLECULAR NMR, 2004)

Status of systems biology - does it have a future? - Naylor, Stephen; Cavanagh, John (Drug Discovery Today: BIOSILICO, 2004)

Sub-classification of response regulators using the surface characteristics of their receiver domains (FEBS 27785) (vol 554, pg 231, 2003) - Kojetin, DJ; Thompson, RJ; Cavanagh, J (FEBS LETTERS, 2004)

Calbindin D-28K interacts with Ran-binding protein M: identification of interacting domains by NMR spectroscopy - Lutz, W; Frank, EM; Craig, TA; Thompson, R; Venters, RA; Kojetin, D; Cavanagh, J; Kumar, R (BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003)

In-line desalting mass spectrometry for the study of noncovalent biological complexes - Cavanagh, J; Benson, LM; Thompson, R; Naylor, S (ANALYTICAL CHEMISTRY, 2003)

Protein-metal ion interactions, stoichiometries and relative affinities determined by on-line size exclusion gel filtration mass spectrometry - Benson, LM; Kumar, R; Cavanagh, J; Naylor, S (RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2003)

Sub-classification of response regulators using the surface characteristics of their receiver domains - Kojetin, DJ; Thompson, RJ; Cavanagh, J (FEBS LETTERS, 2003)
Keywords: OmpR sub-families; homology modeling

The effects of Ca2+ binding on the conformation of calbindin D-28K: A nuclear magnetic resonance and microelectrospray mass spectrometry study - Venters, RA; Benson, LM; Craig, TA; Paul, KH; Kordys, DR; Thompson, R; Naylor, S; Kumar, R; Cavanagh, J (ANALYTICAL BIOCHEMISTRY, 2003)
Keywords: calbindin D-28K; EF-hand; conformational response to calcium binding

Alternative splicing of a beta(4) subunit proline-rich motif regulates voltage-dependent gating and toxin block of Ca(v)2.1 Ca2+ channels - Helton, T. D.; Kojetin, D. J.; Cavanagh, J.; Horne, W. A. (Journal of Neuroscience, 2002)

Current approaches for the study of large proteins by NMR - Venters, RA; Thompson, R; Cavanagh, J (JOURNAL OF MOLECULAR STRUCTURE, 2002)
Keywords: NMR; large proteins; structure; methods

Macromolecular assembly of the transition state regulator AbrB in its unbound and complexed states probed by microelectrospray ionization mass spectrometry - Benson, LM; Vaughn, JL; Strauch, MA; Bobay, BG; Thompson, R; Naylor, S; Cavanagh, J (ANALYTICAL BIOCHEMISTRY, 2002)
Keywords: AbrB; transition-state regulator; macromolecular assembly; mass spectrometry

Stoichiometries of protein - Protein/DNA binding and conformational changes for the transition-state regulator AbrB measured by pseudo cell-size exclusion chromatography-mass spectrometry - Cavanagh, J; Thompson, R; Bobay, B; Benson, LM; Naylor, S (BIOCHEMISTRY, 2002)

Structural and catalytic response to temperature and cosolvents of carboxylesterase EST1 from the extremely thermoacidophilic archaeon Sulfolobus solfataricus P1 - Sehgal, AC; Tompson, R; Cavanagh, J; Kelly, RM (BIOTECHNOLOGY AND BIOENGINEERING, 2002)
Keywords: DMSO; conformational flexibility; enzyme activation; thermostable

Protein dynamic studies move to a new time slot - Cavanagh, J; Venters, RA (NATURE STRUCTURAL BIOLOGY, 2001)

The DNA-binding domain in the Bacillus subtilis transition-state regulator AbrB employs significant motion for promiscuous DNA recognition - Vaughn, JL; Feher, VA; Bracken, C; Cavanagh, J (JOURNAL OF MOLECULAR BIOLOGY, 2001)
Keywords: dynamics; AbrB; transition-state regulator; DNA-binding

Novel DNA binding domain and genetic regulation model of Bacillus subtilis transition state regulator AbrB - Vaughn, J. L.; Feher, V.; Naylor, S.; Strauch, M. A.; Cavanagh, J. (Nature Structural Biology, 2000)

Functional implications of the structure of the bacterial response regulator Spo0F - Feher, V. A.; Zapf, J.; Hoch, J. A.; Whiteley, J. M.; Cavanagh, J.; W., Dahlquist F. (Journal of Cellular Biochemistry, 1995)

Solution structure of the Bacillus subtilis glucose permease Iia domain and its interaction with another phosphocarrier protein Hpr. J (Journal of Cellular Biochemistry, 1993)