Jackie Kalmar

College of Sciences

Works (5)

Updated: July 5th, 2023 15:03

2021 journal article

GlycoHunter: An Open-Source Software for the Detection and Relative Quantification of INLIGHT-Labeled N-Linked Glycans

JOURNAL OF PROTEOME RESEARCH, 20(4), 1855–1863.

By: J. Kalmar n, K. Garrard n & D. Muddiman n 

author keywords: glycomics; INLIGHT; N-linked glycans; data analysis; open source; software
TL;DR: GlycoHunter, a user-friendly software created in MATLAB that enables researchers to accurately and efficiently process MS1 glycomics data where a NAT and SIL pair is generated for relative quantification, including but not limited to, INLIGHT. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: May 3, 2021

2021 article

Utilizing liquid chromatography, ion mobility spectrometry, and mass spectrometry to assess INLIGHT (TM) derivatized N-linked glycans in biological samples

Butler, K. E., Kalmar, J. G., Muddiman, D. C., & Baker, E. S. (2021, August 4). ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol. 8.

By: K. Butler n, J. Kalmar n, D. Muddiman n  & E. Baker n 

author keywords: Glycomics; Ion mobility spectrometry; INLIGHT (TM); N-Linked glycans; LC-IMS-MS
TL;DR: The combination of IMS and the natural (NAT) and stable-isotope label (SIL) in the INLIGHT™ strategy provided additional confidence for each glycan identification due to the mobility aligned NAT- and SIL-labeled glycans and further capabilities for isomer examinations. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 16, 2021

2020 journal article

Comparative Proteomic Analysis of Wild Type and Mutant Lacking an SCF E3 Ligase F-Box Protein in Magnaporthe oryzae

JOURNAL OF PROTEOME RESEARCH, 19(9), 3761–3768.

By: J. Kalmar n, Y. Oh n, R. Dean n  & D. Muddiman n 

MeSH headings : Ascomycota; F-Box Proteins / genetics; Fungal Proteins / genetics; Magnaporthe / metabolism; Proteomics; Ubiquitin-Protein Ligases / genetics
TL;DR: A label-free quantitative global proteomics technique was used to probe the role of ubiquitination and phosphorylation in the mechanism of how E3 ligase regulates change in virulence of M. oryzae and greatly assist in the selection of future genes for functional studies and enabling mechanistic insight related to virulence. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: October 5, 2020

2020 journal article

Enhanced protocol for quantitativeN-linked glycomics analysis using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)(TM)

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 412(27), 7569–7579.

By: J. Kalmar n, K. Butler n, E. Baker n  & D. Muddiman n 

author keywords: Glycomics; Orbitrap; Sample preparation; INLIGHT (TM); N-linked glycans; HILIC; PGC; Reversed-phase; Chromatography
MeSH headings : Chromatography, Reverse-Phase / methods; Female; Fetuins / chemistry; Glycomics / methods; Glycosylation; Humans; Male; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / chemistry; Polysaccharides / analysis; Tandem Mass Spectrometry / methods
TL;DR: To assess the maximum sensitivity and separability of the INLIGHT™ preparation and analysis pipeline, several critical steps were investigated and the identification of low-abundance glycans, such as (Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)3, was possible. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: September 14, 2020

2020 journal article

Investigating host-pathogen meta-metabolic interactions of Magnaporthe oryzae infected barley using infrared matrix-assisted laser desorption electrospray ionization mass spectrometry

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 412(1), 139–147.

By: J. Kalmar n, Y. Oh n, R. Dean n  & D. Muddiman n 

author keywords: IR-MALDESI; Mass spectrometry imaging; Orbitrap; Magnaporthe oryzae; Image recognition; Polarity switching
MeSH headings : Hordeum / metabolism; Hordeum / microbiology; Host-Pathogen Interactions; Infrared Rays; Magnaporthe / metabolism; Magnaporthe / physiology; Metabolomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR: Serotonin, a barley defense metabolite, was a compound identified in both positive and negative modes, and metabolites in the melanin pathway, important for infection development of M. oryzae were identified but were unable to be confirmed with MS/MS due to their low abundances. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: December 9, 2019

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