Jackie Kalmar

College of Sciences

Works (5)

Updated: April 25th, 2025 17:35

2021 article

GlycoHunter: An Open-Source Software for the Detection and Relative Quantification of INLIGHT-Labeled N-Linked Glycans

Kalmar, J. G., Garrard, K. P., & Muddiman, D. C. (2021, January 8). Journal of Proteome Research, Vol. 20, pp. 1855–1863.

By: J. Kalmar n, K. Garrard n & D. Muddiman n 

author keywords: glycomics; INLIGHT; N-linked glycans; data analysis; open source; software
MeSH headings : Computational Biology; Glycomics; Polysaccharides; Software
topics (OpenAlex): Glycosylation and Glycoproteins Research; Carbohydrate Chemistry and Synthesis; Genomics and Phylogenetic Studies
TL;DR: GlycoHunter, a user-friendly software created in MATLAB that enables researchers to accurately and efficiently process MS1 glycomics data where a NAT and SIL pair is generated for relative quantification, including but not limited to, INLIGHT. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: May 3, 2021

2021 article

Utilizing liquid chromatography, ion mobility spectrometry, and mass spectrometry to assess INLIGHT™ derivatized N-linked glycans in biological samples

Butler, K. E., Kalmar, J. G., Muddiman, D. C., & Baker, E. S. (2021, August 4). Analytical and Bioanalytical Chemistry, Vol. 8.

By: K. Butler n, J. Kalmar n, D. Muddiman n  & E. Baker n 

author keywords: Glycomics; Ion mobility spectrometry; INLIGHT (TM); N-Linked glycans; LC-IMS-MS
MeSH headings : Chromatography, Liquid; Glycomics / methods; Glycosylation; Humans; Ion Mobility Spectrometry; Mass Spectrometry / methods; Polysaccharides / analysis
topics (OpenAlex): Glycosylation and Glycoproteins Research; Carbohydrate Chemistry and Synthesis; Genomics and Phylogenetic Studies
TL;DR: The combination of IMS and the natural (NAT) and stable-isotope label (SIL) in the INLIGHT™ strategy provided additional confidence for each glycan identification due to the mobility aligned NAT- and SIL-labeled glycans and further capabilities for isomer examinations. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 16, 2021

2020 article

Comparative Proteomic Analysis of Wild Type and Mutant Lacking an SCF E3 Ligase F-Box Protein in Magnaporthe oryzae

Kalmar, J. G., Oh, Y., Dean, R. A., & Muddiman, D. C. (2020, July 21). Journal of Proteome Research, Vol. 19, pp. 3761–3768.

By: J. Kalmar n, Y. Oh  n, R. Dean n  & D. Muddiman n 

MeSH headings : Ascomycota; F-Box Proteins / genetics; Fungal Proteins / genetics; Magnaporthe / metabolism; Proteomics; Ubiquitin-Protein Ligases / genetics
topics (OpenAlex): Fungal and yeast genetics research; Plant Pathogens and Fungal Diseases; Ubiquitin and proteasome pathways
TL;DR: A label-free quantitative global proteomics technique was used to probe the role of ubiquitination and phosphorylation in the mechanism of how E3 ligase regulates change in virulence of M. oryzae and greatly assist in the selection of future genes for functional studies and enabling mechanistic insight related to virulence. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: October 5, 2020

2020 article

Enhanced protocol for quantitative N-linked glycomics analysis using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™

Kalmar, J. G., Butler, K. E., Baker, E. S., & Muddiman, D. C. (2020, August 26). Analytical and Bioanalytical Chemistry.

By: J. Kalmar n, K. Butler n, E. Baker n  & D. Muddiman n 

author keywords: Glycomics; Orbitrap; Sample preparation; INLIGHT (TM); N-linked glycans; HILIC; PGC; Reversed-phase; Chromatography
MeSH headings : Chromatography, Reverse-Phase / methods; Female; Fetuins / chemistry; Glycomics / methods; Glycosylation; Humans; Male; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / chemistry; Polysaccharides / analysis; Tandem Mass Spectrometry / methods
topics (OpenAlex): Glycosylation and Glycoproteins Research; Carbohydrate Chemistry and Synthesis; Proteoglycans and glycosaminoglycans research
TL;DR: To assess the maximum sensitivity and separability of the INLIGHT™ preparation and analysis pipeline, several critical steps were investigated and the identification of low-abundance glycans, such as (Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)3, was possible. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: September 14, 2020

2019 article

Investigating host-pathogen meta-metabolic interactions of Magnaporthe oryzae infected barley using infrared matrix-assisted laser desorption electrospray ionization mass spectrometry

Kalmar, J. G., Oh, Y., Dean, R. A., & Muddiman, D. C. (2019, November 23). Analytical and Bioanalytical Chemistry, Vol. 412, pp. 139–147.

By: J. Kalmar n, Y. Oh n, R. Dean n  & D. Muddiman n 

author keywords: IR-MALDESI; Mass spectrometry imaging; Orbitrap; Magnaporthe oryzae; Image recognition; Polarity switching
MeSH headings : Hordeum / metabolism; Hordeum / microbiology; Host-Pathogen Interactions; Infrared Rays; Magnaporthe / metabolism; Magnaporthe / physiology; Metabolomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
topics (OpenAlex): Fermentation and Sensory Analysis; Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies
TL;DR: Serotonin, a barley defense metabolite, was a compound identified in both positive and negative modes, and metabolites in the melanin pathway, important for infection development of M. oryzae were identified but were unable to be confirmed with MS/MS due to their low abundances. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: December 9, 2019

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