@article{lu_chandrakanth_lewis_andres_bovet_goepfert_dewey_2021, title={Constitutive activation of nitrate reductase in tobacco alters flowering time and plant biomass}, volume={11}, ISSN={["2045-2322"]}, DOI={10.1038/s41598-021-83797-7}, abstractNote={Abstract}, number={1}, journal={SCIENTIFIC REPORTS}, author={Lu, Jianli and Chandrakanth, Niharika N. and Lewis, Ramsey S. and Andres, Karen and Bovet, Lucien and Goepfert, Simon and Dewey, Ralph E.}, year={2021}, month={Feb} }
 @article{lu_zhang_lewis_bovet_goepfert_jack_crutchfield_ji_dewey_2016, title={Expression of a constitutively active nitrate reductase variant in tobacco reduces tobacco-specific nitrosamine accumulation in cured leaves and cigarette smoke}, volume={14}, ISSN={["1467-7652"]}, DOI={10.1111/pbi.12510}, abstractNote={Summary}, number={7}, journal={PLANT BIOTECHNOLOGY JOURNAL}, author={Lu, Jianli and Zhang, Leichen and Lewis, Ramsey S. and Bovet, Lucien and Goepfert, Simon and Jack, Anne M. and Crutchfield, James D. and Ji, Huihua and Dewey, Ralph E.}, year={2016}, month={Jul}, pages={1500–1510} }
 @article{dong_shew_tredway_lu_sivamani_miller_qu_2008, title={Expression of the bacteriophage T4 lysozyme gene in tall fescue confers resistance to gray leaf spot and brown patch diseases}, volume={17}, ISSN={["1573-9368"]}, DOI={10.1007/s11248-007-9073-3}, abstractNote={Tall fescue (Festuca arundinacea Schreb.) is an important turf and forage grass species worldwide. Fungal diseases present a major limitation in the maintenance of tall fescue lawns, landscapes, and forage fields. Two severe fungal diseases of tall fescue are brown patch, caused by Rhizoctonia solani, and gray leaf spot, caused by Magnaporthe grisea. These diseases are often major problems of other turfgrass species as well. In efforts to obtain tall fescue plants resistant to these diseases, we introduced the bacteriophage T4 lysozyme gene into tall fescue through Agrobacterium-mediated genetic transformation. In replicated experiments under controlled environments conducive to disease development, 6 of 13 transgenic events showed high resistance to inoculation of a mixture of two M. grisea isolates from tall fescue. Three of these six resistant plants also displayed significant resistance to an R. solani isolate from tall fescue. Thus, we have demonstrated that the bacteriophage T4 lysozyme gene confers resistance to both gray leaf spot and brown patch diseases in transgenic tall fescue plants. The gene may have wide applications in engineered fungal disease resistance in various crops.}, number={1}, journal={TRANSGENIC RESEARCH}, author={Dong, Shujie and Shew, H. David and Tredway, Lane P. and Lu, Jianli and Sivamani, Elumalai and Miller, Eric S. and Qu, Rongda}, year={2008}, month={Feb}, pages={47–57} }
 @article{lu_sivamani_azhakanandam_samadder_li_qu_2008, title={Gene expression enhancement mediated by the 5 ' UTR intron of the rice rubi3 gene varied remarkably among tissues in transgenic rice plants}, volume={279}, ISSN={["1617-4615"]}, DOI={10.1007/s00438-008-0333-6}, abstractNote={Introns are important sequence elements that modulate the expression of genes. Using the GUS reporter gene driven by the promoter of the rice (Oryza sativa L.) polyubiquitin rubi3 gene, we investigated the effects of the 5' UTR intron of the rubi3 gene and the 5' terminal 27 bp of the rubi3 coding sequence on gene expression in stably transformed rice plants. While the intron enhanced GUS gene expression, the 27-bp fused to the GUS coding sequence further augmented GUS expression level, with both varying among different tissues. The intron elevated GUS gene expression mainly at mRNA accumulation level, but also stimulated enhancement at translational level. The enhancement on mRNA accumulation, as determined by realtime quantitative RT-PCR, varied remarkably with tissue type. The augmentation by the intron at translational level also differed by tissue type, but to a lesser extent. On the other hand, the 27-bp fusion further boosted GUS protein yield without affecting mRNA accumulation level, indicating stimulation at translation level, which was also affected by tissue type. The research revealed substantial variation in the magnitudes of intron-mediated enhancement of gene expression (IME) among tissues in rice plants and the importance of using transgenic plants for IME studies.}, number={6}, journal={MOLECULAR GENETICS AND GENOMICS}, author={Lu, Jianli and Sivamani, Elumalai and Azhakanandam, Kasi and Samadder, Partha and Li, Xianggan and Qu, Rongda}, year={2008}, month={Jun}, pages={563–572} }
 @article{samadder_sivamani_lu_li_qu_2008, title={Transcriptional and post-transcriptional enhancement of gene expression by the 5 ' UTR intron of rice rubi3 gene in transgenic rice cells}, volume={279}, ISSN={["1617-4623"]}, DOI={10.1007/s00438-008-0323-8}, abstractNote={Introns play a very important role in regulating gene expression in eukaryotes. In plants, many introns enhance gene expression, and the effect of intron-mediated enhancement (IME) of gene expression is reportedly often more profound in monocots than in dicots. To further gain insight of IME in monocot plants, we quantitatively dissected the effect of the 5' UTR intron of the rice rubi3 gene at various gene expression levels in stably transformed suspension cell lines. The intron enhanced the GUS reporter gene activity in these lines by about 29-fold. Nuclear run-on experiments demonstrated a nearly twofold enhancement by the 5' UTR intron at the transcriptional level. RNA analysis by RealTime quantitative RT-PCR assays indicated the intron enhanced the steady state RNA level of the GUS reporter gene by nearly 20-fold, implying a strong role of the intron in RNA processing and/or export. The results also implicated a moderate role of the intron in enhancement at the translational level ( approximately 45%). Moreover, results from a transient assay experiment using a shortened exon 1 sequence revealed an important role of exon 1 of rubi3 in gene expression. It may also hint a divergence in IME mechanisms between plant and animal cells. These results demonstrated transcriptional enhancement by a plant intron, but suggested that post-transcriptional event(s) be the major source of IME.}, number={4}, journal={MOLECULAR GENETICS AND GENOMICS}, author={Samadder, Partha and Sivamani, Eltunalai and Lu, Jianli and Li, Xianggan and Qu, Rongda}, year={2008}, month={Apr}, pages={429–439} }