Jonathan Michael Conway

Works (10)

Updated: July 5th, 2023 15:42

2019 journal article

Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii

NATURE COMMUNICATIONS, 10(1).

By: C. Straub n, P. Khatibi n, J. Wang n , J. Conway n, A. Williams-Rhaesa *, I. Peszlen n , V. Chiang n, M. Adams *, R. Kelly n 

Contributors: C. Straub n, P. Khatibi n, J. Wang n , J. Conway n, A. Williams-Rhaesa *, I. Peszlen n , V. Chiang n, M. Adams *, R. Kelly n 

MeSH headings : Biomass; Cellulose / metabolism; Clostridiales / genetics; Clostridiales / metabolism; Ethanol / metabolism; Fermentation; Industrial Microbiology; Lignin / metabolism; Metabolic Engineering; Plants, Genetically Modified / chemistry; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Polysaccharides / metabolism; Populus / chemistry; Populus / genetics; Populus / metabolism
TL;DR: The authors pair transgenic lignin modified poplar lines with engineered Caldicellusiruptor bescii to achieve biomass solubilization and ethanol conversion without pretreatment and the nearly quantitative microbial conversion of the carbohydrate content of unpretreated transgen lignocellulosic biomass bodes well for full utilization of renewable biomass feedstocks. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 26, 2019

2017 journal article

Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(17).

By: J. Zurawski n, P. Khatibi n, H. Akinosho *, C. Straub n, S. Compton n, J. Conway n, L. Lee n, A. Ragauskas * ...

author keywords: Caldicellulosiruptor; switchgrass; lignocellulose deconstruction and conversion; extreme thermophiles; lignocellulose
MeSH headings : Biomass; Fermentation; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / metabolism; Hot Temperature; Lignin / chemistry; Lignin / metabolism; Panicum / chemistry; Panicum / genetics; Panicum / metabolism; Panicum / microbiology; Plants, Genetically Modified / chemistry; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Plants, Genetically Modified / microbiology; Polysaccharides / chemistry; Polysaccharides / metabolism
TL;DR: While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

Functional Analysis of the Glucan Degradation Locus in Caldicellulosiruptor bescii Reveals Essential Roles of Component Glycoside Hydrolases in Plant Biomass Deconstruction

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(24).

By: J. Conway n, B. McKinley n, N. Seals n, D. Hernandez n, P. Khatibi  n, S. Poudel *, R. Giannone *, R. Hettich * ...

author keywords: Caldicellulosiruptor; extreme thermophile; cellulose degradation; lignocellulose; glycoside hydrolase; cellulase
MeSH headings : Bacterial Proteins / metabolism; Cellulose / chemistry; Firmicutes / genetics; Firmicutes / metabolism; Glucans / metabolism; Glycoside Hydrolases / metabolism; Panicum / chemistry; Populus / chemistry
TL;DR: It is demonstrated that certain GDL GHs are primarily responsible for the degradation of microcrystalline cellulose-containing substrates by C. bescii and provide new insights into the workings of a novel microbial mechanism for lignocellulose utilization. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

Genome Stability in Engineered Strains of the Extremely Thermophilic Lignocellulose-Degrading Bacterium Caldicellulosiruptor bescii

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(14).

By: A. Williams-Rhaesa *, F. Poole*, J. Dinsmore*, G. Lipscomb*, G. Rubinstein*, I. Scott*, J. Conway n, L. Lee n ...

author keywords: Southern blotting; genome sequencing; insertion elements; kanamycin resistance
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Genetic Engineering; Genome, Bacterial; Genomic Instability; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / metabolism; Hot Temperature; Lignin / metabolism
TL;DR: It is shown that strains derived from the genetic background containing a random deletion in uracil biosynthesis genes (pyrFA) have a dramatic increase in the number of single nucleotide polymorphisms, insertions/deletions, and ISCbe4 insertion elements in their genomes compared to the wild type. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

A Highly Thermostable Kanamycin Resistance Marker Expands the Tool Kit for Genetic Manipulation of Caldicellulosiruptor bescii

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(14), 4421–4428.

By: G. Lipscomb*, J. Conway n, S. Blumer-Schuette n, R. Kelly n  & M. Adams *

MeSH headings : Firmicutes / genetics; Firmicutes / radiation effects; Genetics, Microbial / methods; Genomic Instability / radiation effects; Hot Temperature; Kanamycin Resistance; Molecular Biology / methods; Selection, Genetic
TL;DR: A selection system for C. bescii using the antibiotic kanamycin is developed and it is shown that, in combination with the biosynthetic gene marker, it can be used to efficiently delete genes in this organism. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

Multidomain, Surface Layer-associated Glycoside Hydrolases Contribute to Plant Polysaccharide Degradation by Caldicellulosiruptor Species

JOURNAL OF BIOLOGICAL CHEMISTRY, 291(13), 6732–6747.

By: J. Conway n, W. Pierce n, J. Le n, G. Harper n, J. Wright n, A. Tucker n, J. Zurawski n, L. Lee n, S. Blumer-Schuette n, R. Kelly n 

author keywords: biofuel; cell surface; cell surface enzyme; enzyme; glycoside hydrolase; plant cell wall; Caldicellulosiruptor; S-layer; lignocellulose
MeSH headings : Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Cloning, Molecular; Clostridiales / chemistry; Clostridiales / classification; Clostridiales / enzymology; Escherichia coli / genetics; Escherichia coli / metabolism; Gene Expression; Genome, Bacterial; Glucans / metabolism; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Kinetics; Mutation; Phylogeny; Polysaccharides / metabolism; Protein Binding; Protein Engineering; Protein Structure, Tertiary; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Substrate Specificity; Wood / metabolism; Xylans / metabolism
TL;DR: The results here provide new insights into the architecture and role of SLHdomain GHs and demonstrate that hemicellulose degradation can be enhanced through non-native SLH domain GHs engineered into the genomes of Caldicelluosiruptor species. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Comparative Analysis of Extremely Thermophilic Caldicellulosiruptor Species Reveals Common and Unique Cellular Strategies for Plant Biomass Utilization

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 81(20), 7159–7170.

By: J. Zurawski n, J. Conway n, L. Lee n, H. Simpson  n, J. Izquierdo  n, S. Blumer-Schuette n, I. Nookaew *, M. Adams *, R. Kelly n 

MeSH headings : Bacteria / metabolism; Biomass; Cellulase / metabolism; Panicum / enzymology; Panicum / metabolism; Plants / enzymology
TL;DR: Results here show that capacity for plant biomass deconstruction varies across Caldicellulosiruptor species and depends in a complex way on GH genome inventory, substrate composition, and gene regulation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Discrete and Structurally Unique Proteins (Tapirins) Mediate Attachment of Extremely Thermophilic Caldicellulosiruptor Species to Cellulose

JOURNAL OF BIOLOGICAL CHEMISTRY, 290(17), 10645–10656.

By: S. Blumer-Schuette n, M. Alahuhta *, J. Conway n, L. Lee n, J. Zurawski n, R. Giannone *, R. Hettich *, V. Lunin *, M. Himmel *, R. Kelly n 

MeSH headings : Adsorption; Bacteria / genetics; Bacteria / metabolism; Bacteria / ultrastructure; Bacterial Adhesion / physiology; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Binding Sites; Cellulose / metabolism; Fimbriae, Bacterial / metabolism; Genes, Bacterial; Models, Molecular; Phylogeny; Plants / microbiology; Protein Structure, Tertiary; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism
TL;DR: Structurally unique, discrete proteins (tāpirins), not directly associated with cellulases, that mediate attachment to cellulose by species in the noncellulosomal, extremely thermophilic bacterial genus Caldicellulosiruptor are identified and define a new class of polysaccharide adhesins. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 review

Thermophilic lignocellulose deconstruction

[Review of ]. FEMS MICROBIOLOGY REVIEWS, 38(3), 393–448.

By: S. Blumer-Schuette n, S. Brown *, K. Sander *, E. Bayer*, I. Kataeva*, J. Zurawski n, J. Conway n, M. Adams *, R. Kelly n 

author keywords: thermophilic microorganisms; cellulosome; lignocellulose deconstruction; carbohydrate-active enzymes; systems biology; bioenergy
MeSH headings : Biofuels; Cellulosomes / genetics; Cellulosomes / metabolism; Gram-Positive Endospore-Forming Bacteria / classification; Gram-Positive Endospore-Forming Bacteria / enzymology; Gram-Positive Endospore-Forming Bacteria / genetics; Hot Temperature; Lignin / metabolism; Molecular Sequence Data; Plants / metabolism; Soil Microbiology
TL;DR: Plant-biomass-deconstructing thermophilic bacteria from the genera Clostridium and Caldicellulosiruptor, which have potential as metabolic engineering platforms for producing biofuels, are compared and contrasted from a systems biology perspective. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

journal article

Lignocellulosic biomass deconstruction by the extremely thermophilic genus caldicellulosiruptor

Conway, J. M., Zurawski, J. V., Lee, L. L., Blumer-Schuette, S. E., & Kelly, R. M. Thermophilic Microorganisms, 91–119.

By: J. Conway, J. Zurawski, L. Lee, S. Blumer-Schuette & R. Kelly 

Source: NC State University Libraries
Added: August 6, 2018

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