Jonathan Michael Conway

Works (10)

Updated: July 5th, 2023 15:42

2019 article

Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii

Straub, C. T., Khatibi, P. A., Wang, J. P., Conway, J. M., Williams-Rhaesa, A. M., Peszlen, I. M., … Kelly, R. M. (2019, August 7). Nature Communications, Vol. 10.

By: C. Straub n, P. Khatibi n, J. Wang n , J. Conway n, A. Williams-Rhaesa *, I. Peszlen n , V. Chiang n, M. Adams *, R. Kelly n 

Contributors: C. Straub n, P. Khatibi n, J. Wang n , J. Conway n, A. Williams-Rhaesa *, I. Peszlen n , V. Chiang n, M. Adams *, R. Kelly n 

MeSH headings : Biomass; Cellulose / metabolism; Clostridiales / genetics; Clostridiales / metabolism; Ethanol / metabolism; Fermentation; Industrial Microbiology; Lignin / metabolism; Metabolic Engineering; Plants, Genetically Modified / chemistry; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Polysaccharides / metabolism; Populus / chemistry; Populus / genetics; Populus / metabolism
topics (OpenAlex): Biofuel production and bioconversion; Microbial Metabolic Engineering and Bioproduction; Catalysis for Biomass Conversion
TL;DR: The authors pair transgenic lignin modified poplar lines with engineered Caldicellusiruptor bescii to achieve biomass solubilization and ethanol conversion without pretreatment and the nearly quantitative microbial conversion of the carbohydrate content of unpretreated transgen lignocellulosic biomass bodes well for full utilization of renewable biomass feedstocks. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 26, 2019

2017 article

Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii

Zurawski, J. V., Khatibi, P. A., Akinosho, H. O., Straub, C. T., Compton, S. H., Conway, J. M., … Kelly, R. M. (2017, June 17). Applied and Environmental Microbiology, Vol. 83.

By: J. Zurawski n, P. Khatibi n, H. Akinosho *, C. Straub n, S. Compton n, J. Conway n, L. Lee n, A. Ragauskas * ...

author keywords: Caldicellulosiruptor; switchgrass; lignocellulose deconstruction and conversion; extreme thermophiles; lignocellulose
MeSH headings : Biomass; Fermentation; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / metabolism; Hot Temperature; Lignin / chemistry; Lignin / metabolism; Panicum / chemistry; Panicum / genetics; Panicum / metabolism; Panicum / microbiology; Plants, Genetically Modified / chemistry; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Plants, Genetically Modified / microbiology; Polysaccharides / chemistry; Polysaccharides / metabolism
topics (OpenAlex): Biofuel production and bioconversion; Catalysis for Biomass Conversion; Lignin and Wood Chemistry; Advanced Cellulose Research Studies
TL;DR: While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 article

Functional Analysis of the Glucan Degradation Locus in Caldicellulosiruptor bescii Reveals Essential Roles of Component Glycoside Hydrolases in Plant Biomass Deconstruction

Conway, J. M., McKinley, B. S., Seals, N. L., Hernandez, D., Khatibi, P. A., Poudel, S., … Kelly, R. M. (2017, October 7). Applied and Environmental Microbiology, Vol. 83.

By: J. Conway n, B. McKinley n, N. Seals n, D. Hernandez n, P. Khatibi  n, S. Poudel *, R. Giannone *, R. Hettich * ...

author keywords: Caldicellulosiruptor; extreme thermophile; cellulose degradation; lignocellulose; glycoside hydrolase; cellulase
MeSH headings : Bacterial Proteins / metabolism; Cellulose / chemistry; Firmicutes / genetics; Firmicutes / metabolism; Glucans / metabolism; Glycoside Hydrolases / metabolism; Panicum / chemistry; Populus / chemistry
topics (OpenAlex): Biofuel production and bioconversion; Enzyme Production and Characterization; Microbial Metabolic Engineering and Bioproduction
TL;DR: It is demonstrated that certain GDL GHs are primarily responsible for the degradation of microcrystalline cellulose-containing substrates by C. bescii and provide new insights into the workings of a novel microbial mechanism for lignocellulose utilization. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 article

Genome Stability in Engineered Strains of the Extremely Thermophilic Lignocellulose-Degrading Bacterium Caldicellulosiruptor bescii

Williams-Rhaesa, A. M., Poole, F. L., Dinsmore, J. T., Lipscomb, G. L., Rubinstein, G. M., Scott, I. M., … Adams, M. W. W. (2017, May 6). Applied and Environmental Microbiology, Vol. 83.

By: A. Williams-Rhaesa *, F. Poole*, J. Dinsmore*, G. Lipscomb*, G. Rubinstein *, I. Scott*, J. Conway n, L. Lee n ...

author keywords: Southern blotting; genome sequencing; insertion elements; kanamycin resistance
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Genetic Engineering; Genome, Bacterial; Genomic Instability; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / metabolism; Hot Temperature; Lignin / metabolism
topics (OpenAlex): Biofuel production and bioconversion; Genomics and Phylogenetic Studies; Enzyme Production and Characterization
TL;DR: It is shown that strains derived from the genetic background containing a random deletion in uracil biosynthesis genes (pyrFA) have a dramatic increase in the number of single nucleotide polymorphisms, insertions/deletions, and ISCbe4 insertion elements in their genomes compared to the wild type. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 article

A Highly Thermostable Kanamycin Resistance Marker Expands the Tool Kit for Genetic Manipulation of Caldicellulosiruptor bescii

Lipscomb, G. L., Conway, J. M., Blumer-Schuette, S. E., Kelly, R. M., & Adams, M. W. W. (2016, May 14). Applied and Environmental Microbiology, Vol. 82, pp. 4421–4428.

By: G. Lipscomb*, J. Conway n, S. Blumer-Schuette n, R. Kelly n  & M. Adams *

MeSH headings : Firmicutes / genetics; Firmicutes / radiation effects; Genetics, Microbial / methods; Genomic Instability / radiation effects; Hot Temperature; Kanamycin Resistance; Molecular Biology / methods; Selection, Genetic
topics (OpenAlex): CRISPR and Genetic Engineering; Insect symbiosis and bacterial influences; Parasitic Diseases Research and Treatment
TL;DR: A selection system for C. bescii using the antibiotic kanamycin is developed and it is shown that, in combination with the biosynthetic gene marker, it can be used to efficiently delete genes in this organism. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 article

Multidomain, Surface Layer-associated Glycoside Hydrolases Contribute to Plant Polysaccharide Degradation by Caldicellulosiruptor Species

Conway, J. M., Pierce, W. S., Le, J. H., Harper, G. W., Wright, J. H., Tucker, A. L., … Kelly, R. M. (2016, January 27). Journal of Biological Chemistry, Vol. 291, pp. 6732–6747.

By: J. Conway n, W. Pierce n, J. Le n, G. Harper n, J. Wright n, A. Tucker n, J. Zurawski n, L. Lee n, S. Blumer-Schuette n, R. Kelly n 

author keywords: biofuel; cell surface; cell surface enzyme; enzyme; glycoside hydrolase; plant cell wall; Caldicellulosiruptor; S-layer; lignocellulose
MeSH headings : Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Cloning, Molecular; Clostridiales / chemistry; Clostridiales / classification; Clostridiales / enzymology; Escherichia coli / genetics; Escherichia coli / metabolism; Gene Expression; Genome, Bacterial; Glucans / metabolism; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Kinetics; Mutation; Phylogeny; Polysaccharides / metabolism; Protein Binding; Protein Engineering; Protein Structure, Tertiary; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Substrate Specificity; Wood / metabolism; Xylans / metabolism
topics (OpenAlex): Enzyme Production and Characterization; Biofuel production and bioconversion; Studies on Chitinases and Chitosanases
TL;DR: The results here provide new insights into the architecture and role of SLHdomain GHs and demonstrate that hemicellulose degradation can be enhanced through non-native SLH domain GHs engineered into the genomes of Caldicelluosiruptor species. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 article

Comparative Analysis of Extremely Thermophilic Caldicellulosiruptor Species Reveals Common and Unique Cellular Strategies for Plant Biomass Utilization

Zurawski, J. V., Conway, J. M., Lee, L. L., Simpson, H. J., Izquierdo, J. A., Blumer-Schuette, S., … Kelly, R. M. (2015, August 8). Applied and Environmental Microbiology, Vol. 81, pp. 7159–7170.

By: J. Zurawski n, J. Conway n, L. Lee n, H. Simpson  n, J. Izquierdo  n, S. Blumer-Schuette n, I. Nookaew *, M. Adams *, R. Kelly n 

MeSH headings : Bacteria / metabolism; Biomass; Cellulase / metabolism; Panicum / enzymology; Panicum / metabolism; Plants / enzymology
topics (OpenAlex): Biofuel production and bioconversion; Microbial Metabolic Engineering and Bioproduction; Anaerobic Digestion and Biogas Production
TL;DR: Results here show that capacity for plant biomass deconstruction varies across Caldicellulosiruptor species and depends in a complex way on GH genome inventory, substrate composition, and gene regulation. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 article

Discrete and Structurally Unique Proteins (Tāpirins) Mediate Attachment of Extremely Thermophilic Caldicellulosiruptor Species to Cellulose

Blumer-Schuette, S. E., Alahuhta, M., Conway, J. M., Lee, L. L., Zurawski, J. V., Giannone, R. J., … Kelly, R. M. (2015, February 27). Journal of Biological Chemistry, Vol. 290, pp. 10645–10656.

By: S. Blumer-Schuette n, M. Alahuhta *, J. Conway n, L. Lee n, J. Zurawski n, R. Giannone *, R. Hettich *, V. Lunin *, M. Himmel *, R. Kelly n 

MeSH headings : Adsorption; Bacteria / genetics; Bacteria / metabolism; Bacteria / ultrastructure; Bacterial Adhesion / physiology; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Binding Sites; Cellulose / metabolism; Fimbriae, Bacterial / metabolism; Genes, Bacterial; Models, Molecular; Phylogeny; Plants / microbiology; Protein Structure, Tertiary; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism
topics (OpenAlex): Biofuel production and bioconversion; Enzyme Catalysis and Immobilization; Calcium signaling and nucleotide metabolism
TL;DR: Structurally unique, discrete proteins (tāpirins), not directly associated with cellulases, that mediate attachment to cellulose by species in the noncellulosomal, extremely thermophilic bacterial genus Caldicellulosiruptor are identified and define a new class of polysaccharide adhesins. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 article

Thermophilic lignocellulose deconstruction

Blumer-Schuette, S. E., Brown, S. D., Sander, K. B., Bayer, E. A., Kataeva, I., Zurawski, J. V., … Kelly, R. M. (2013, October 12). FEMS Microbiology Reviews, Vol. 38, pp. 393–448.

By: S. Blumer-Schuette n, S. Brown *, K. Sander *, E. Bayer *, I. Kataeva*, J. Zurawski n, J. Conway n, M. Adams *, R. Kelly n 

author keywords: thermophilic microorganisms; cellulosome; lignocellulose deconstruction; carbohydrate-active enzymes; systems biology; bioenergy
MeSH headings : Biofuels; Cellulosomes / genetics; Cellulosomes / metabolism; Gram-Positive Endospore-Forming Bacteria / classification; Gram-Positive Endospore-Forming Bacteria / enzymology; Gram-Positive Endospore-Forming Bacteria / genetics; Hot Temperature; Lignin / metabolism; Molecular Sequence Data; Plants / metabolism; Soil Microbiology
topics (OpenAlex): Biofuel production and bioconversion; Enzyme Production and Characterization; Microbial Metabolic Engineering and Bioproduction
TL;DR: Plant-biomass-deconstructing thermophilic bacteria from the genera Clostridium and Caldicellulosiruptor, which have potential as metabolic engineering platforms for producing biofuels, are compared and contrasted from a systems biology perspective. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

journal article

Lignocellulosic biomass deconstruction by the extremely thermophilic genus caldicellulosiruptor

Conway, J. M., Zurawski, J. V., Lee, L. L., Blumer-Schuette, S. E., & Kelly, R. M. Thermophilic Microorganisms, 91–119.

By: J. Conway, J. Zurawski, L. Lee, S. Blumer-Schuette & R. Kelly 

Source: NC State University Libraries
Added: August 6, 2018

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