John Martin Freund

Works (12)

Updated: January 17th, 2025 09:57

2024 journal article

Assessment of equine intestinal epithelial junctional complexes and barrier permeability using a monolayer culture system

FRONTIERS IN VETERINARY SCIENCE, 11.

By: A. Stewart n , J. Kopper *, C. McKinney-Aguirre n , B. Veerasamy n, D. Sahoo *, J. Freund n, L. Gonzalez n 

author keywords: claudin; horse; jejunum; occludin; scratch assay; transepithelial resistance
Sources: Web Of Science, NC State University Libraries
Added: November 18, 2024

2024 journal article

Large animal models enhance the study of crypt-mediated epithelial recovery from prolonged intestinal ischemia reperfusion injury

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 327(6), G783–G788.

By: C. McKinney-Aguirre , C. Schaaf n, E. Goya-Jorge  n, J. Freund n & L. Gonzalez n 

author keywords: intestinal ischemia reperfusion injury; intestinal stem cell; mouse model; porcine model
Sources: Web Of Science, ORCID, NC State University Libraries
Added: November 13, 2024

2023 journal article

45: Comparison of the Effects of Normothermic Machine Perfusion and Cold Storage Preservation on Porcine Intestinal Allograft Regenerative Potential and Viability

Transplantation, 107(7S), 25–25.

By: E. Ludwig n, N. Abraham *, C. McKinney  n, J. Freund n, A. Stewart n , K. Garman *, A. Barbas *, D. Sudan *, L. Gonzalez n 

Contributors: E. Ludwig n, N. Abraham *, C. McKinney  n, J. Freund n, A. Stewart n , K. Garman *, A. Barbas *, D. Sudan *, L. Gonzalez n 

Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: July 31, 2023

2023 journal article

A LGR5 reporter pig model closely resembles human intestine for improved study of stem cells in disease

FASEB JOURNAL, 37(6).

By: C. Schaaf n, K. Polkoff n, A. Carter n, A. Stewart n , B. Sheahan n , J. Freund n, J. Ginzel *, J. Snyder * ...

author keywords: APC mutation; colorectal cancer; intestine; Lgr5; porcine; stem cell; transgenic large animal
MeSH headings : Humans; Swine; Animals; Mice; In Situ Hybridization, Fluorescence; Intestines; Stem Cells; Ileum; Colon; Coleoptera; Green Fluorescent Proteins / genetics; Receptors, G-Protein-Coupled / genetics
TL;DR: This study validates ISC isolation in a new porcine Leucine Rich Repeat Containing G Protein‐Coupled Receptor 5 (LGR5) reporter line and demonstrates the use of these pigs as a novel colorectal cancer model. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: June 12, 2023

2023 journal article

Changes in equine intestinal stem/progenitor cell number at resection margins in cases of small intestinal strangulation

Equine Veterinary Journal, 55(6), 995–1002.

By: B. Veerasammy  n, G. Gonzalez n, P. Báez‐Ramos n, C. Schaaf n, A. Stewart n , E. Ludwig n, C. McKinney‐Aguirre, J. Freund n, J. Robertson n , L. Gonzalez n 

Contributors: B. Veerasammy  n, G. Gonzalez n, P. Baez-Ramos n, C. Schaaf n, A. Stewart n , E. Ludwig n, C. McKinney-Aguirre , J. Freund n, J. Robertson n , L. Gonzalez n 

author keywords: horse; immunofluorescence; intestine; Ki67; small intestinal strangulation; stem cell
TL;DR: Proliferating cell and ISC numbers may be associated with case outcome, and associations between biomarker expression and the number of preoperative risk factors and postoperative complications negatively related to outcome were determined. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: January 31, 2023

2023 journal article

Comparison of the effects of normothermic machine perfusion and cold storage preservation on porcine intestinal allograft regenerative potential and viability

American Journal of Transplantation, 24(4), 564–576.

By: E. Ludwig n, N. Abraham *, C. Schaaf n, C. McKinney  n, J. Freund n, A. Stewart n , B. Veerasammy  n, M. Thomas  n ...

Contributors: E. Ludwig n, N. Abraham *, C. Schaaf n, C. McKinney  n, J. Freund n, A. Stewart n , B. Veerasammy  n, M. Thomas  n ...

author keywords: intestinal transplantation; normothermic machine perfusion; cold storage; ischemia-reperfusion injury; intestinal stem cells; preservation injury
TL;DR: Normothermic machine perfusion preservation of kidney and liver allografts minimizes CS-associated ischemia-reperfusion injury and appears to reduce IRI and improve graft regeneration with improved ISC viability and proliferation. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: December 28, 2023

2022 journal article

Culture of equine intestinal epithelial stem cells after delayed tissue storage for future applications

BMC VETERINARY RESEARCH, 18(1).

By: A. Stewart n , C. Schaaf n, B. Veerasammy  n, J. Freund n & L. Gonzalez n 

author keywords: Horse; Stem cell; 3D culture; Organoid; Enteroid; Intestine; Storage
MeSH headings : Animals; Cryopreservation / veterinary; Epithelial Cells / cytology; Horses; Intestines / cytology; Stem Cells / cytology; Time Factors; Cells, Cultured; Intestinal Diseases / therapy; Intestinal Diseases / veterinary
TL;DR: Equine ISCs can be isolated and cultured after prolonged tissue storage and this suggests that ISCs could be isolated for several days from samples properly stored after procedures, including surgery or necropsy, and used to create ISC repositories for study or therapy of equine intestinal diseases. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: January 30, 2023

2022 journal article

Orthotopic Transplantation of the Full-length Porcine Intestine After Normothermic Machine Perfusion

Transplantation Direct, 8(11), e1390.

By: N. Abraham *, E. Ludwig n, C. Schaaf n, B. Veerasammy  n, A. Stewart n , C. McKinney  n, J. Freund n, J. Brassil ...

Contributors: N. Abraham *, E. Ludwig n, C. Schaaf n, B. Veerasammy  n, A. Stewart n , C. McKinney  n, J. Freund n, J. Brassil ...

TL;DR: Development and optimization of machine perfusion preservation of small intestine and successful transplantation of intestinal allografts in a porcine model are reported. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: October 31, 2022

2021 journal article

HOPX+ injury-resistant intestinal stem cells drive epithelial recovery after severe intestinal ischemia

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 321(5), G588–G602.

By: A. Stewart n , C. Schaaf n, J. Luff n, J. Freund n, T. Becker *, S. Tufts n, J. Robertson n , L. Gonzalez n 

author keywords: epithelial repair; HOPX; ischemia; large animal models; stem cell
MeSH headings : Animals; Cell Proliferation; Disease Models, Animal; Epithelial Cells / metabolism; Epithelial Cells / pathology; Female; Homeodomain Proteins / genetics; Homeodomain Proteins / metabolism; Intestinal Mucosa / metabolism; Intestinal Mucosa / pathology; Male; Mesenteric Ischemia / genetics; Mesenteric Ischemia / metabolism; Mesenteric Ischemia / pathology; Phenotype; Re-Epithelialization; Severity of Illness Index; Stem Cells / metabolism; Stem Cells / pathology; Sus scrofa; Tissue Culture Techniques
TL;DR: It is demonstrated that during early in vivo recovery, injury-resistant HOPX+cells maintain quiescence, suggesting that HopX may serve a functional role in ISC mediated regeneration after injury and could be a target to control ISC proliferation. (via Semantic Scholar)
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3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: November 3, 2021

2019 journal article

Enteroendocrine Progenitor Cell-Enriched mir-7 Regulates Intestinal Epithelial Proliferation in an Xiap-Dependent Manner

CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY, 9(3), 447–464.

By: A. Singh *, Y. Hung*, M. Shanahan *, M. Kanke *, A. Bonfini *, M. Dame *, M. Biraud n, B. Peck * ...

author keywords: miR-7; Enteroendocrine Lineage; Small Intestine; Enteroid; Proliferation
MeSH headings : Animals; Cell Lineage / genetics; Cell Proliferation / genetics; Cells, Cultured; Computational Biology; Enteroendocrine Cells / physiology; ErbB Receptors / metabolism; Feeding Behavior / physiology; Female; Inhibitor of Apoptosis Proteins / genetics; Inhibitor of Apoptosis Proteins / metabolism; Intestinal Mucosa / cytology; Intestinal Mucosa / physiology; Male; Mice; Mice, Transgenic; MicroRNAs / metabolism; Models, Animal; Organoids; Primary Cell Culture; RNA-Seq; Signal Transduction / genetics; Single-Cell Analysis; Stem Cells / physiology
TL;DR: This study demonstrates for the first time that EEC progenitor cell-enriched miR-7 is altered by dietary perturbations and that it regulates growth in enteroids via intact Xiap and Egfr signaling. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: April 6, 2020

2018 journal article

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

Journal of Visualized Experiments, 5(135).

By: A. Stieler Stewart  n, J. Freund n, A. Blikslager n  & L. Gonzalez n 

author keywords: Medicine; Issue 135; Porcine; intestine; ischemia-reperfusion; stem cell; enteroid; organoid
MeSH headings : Animals; Cell Culture Techniques / methods; Disease Models, Animal; Humans; Intestine, Small / cytology; Intestine, Small / metabolism; Intestine, Small / pathology; Ischemia / etiology; Mice; Stem Cells / metabolism; Swine
TL;DR: By utilizing a porcine model, a protocol is established in which segmental loops of intestinal ischemia can be created within a single animal, enabling the study of differing time points of ischemic injury and repair in vivo. (via Semantic Scholar)
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3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: October 16, 2018

2017 journal article

Advanced three-dimensional culture of equine intestinal epithelial stem cells

Equine Veterinary Journal, 50(2), 241–248.

By: A. Stewart  n, J. Freund n & L. Gonzalez n 

author keywords: horse; stem cell; 3D culture; intestine; enteroid; organoid
MeSH headings : Animals; Cell Culture Techniques / veterinary; Cell Differentiation; Female; Horses; Intestinal Mucosa / cytology; Male; Models, Biological; Stem Cells / physiology
TL;DR: The successful culture of equine intestinal crypts into 3D "mini-guts" allows for in vitro studies of the equine intestine and has relevance to future development of novel therapies that harness the regenerative potential ofequine intestine in horses with gastrointestinal disease (colic). (via Semantic Scholar)
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3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

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