Works (3)

Updated: April 5th, 2024 08:23

2023 article

Peptide ligands for the affinity purification of adeno-associated viruses from HEK 293 cell lysates

Chu, W., Shastry, S., Barbieri, E., Prodromou, R., Greback-Clarke, P., Smith, W., … Menegatti, S. (2023, July 12). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 7.

By: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback-Clarke n, W. Smith n, B. Moore n, R. Kilgore n ...

author keywords: adeno-associated virus; affinity chromatography; HEK 293 lysate; peptide ligands; virus purification
MeSH headings : Humans; Dependovirus / genetics; HEK293 Cells; Ligands; Peptides / genetics; Peptides / metabolism; Amino Acid Sequence; Genetic Vectors
TL;DR: Peptide ligands that selectively capture AAVs and release them under mild conditions are introduced, affording high recovery, high recovery of host cell proteins (HCPs), and high transduction activity of the purified viruses. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 7, 2023

2023 article

Rational design and experimental evaluation of peptide ligands for the purification of adeno-associated viruses via affinity chromatography

Shastry, S., Chu, W., Barbieri, E., Greback-Clarke, P., Smith, W. K., Cummings, C., … Menegatti, S. (2023, September 28). BIOTECHNOLOGY JOURNAL, Vol. 9.

By: S. Shastry n, W. Chu n, E. Barbieri n, P. Greback-Clarke n, W. Smith n, C. Cummings n, A. Minzoni n, J. Pancorbo n ...

author keywords: adeno-associated virus; affinity chromatography; gene therapy; peptide ligands; transduction activity
TL;DR: This study introduces a cohort of peptide ligands that mimic the biorecognition activity of the AAV receptor (AAVR) and anti-AAV antibody A20, enable product elution under near-physiological conditions (pH 6.0) and grant extended reusability by withstanding multiple regenerations. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: October 10, 2023

2014 journal article

Comprehensive Hands-on Training for Influenza Vaccine Manufacturing: A WHO-BARDA-BTEC Partnership for Global Workforce Development

BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION, 42(5), 414–419.

By: J. Ruiz n, G. Gilleskie n, P. Brown n, B. Burnett* & R. Carbonell n

author keywords: workforce development; developing countries vaccine manufacturers; influenza vaccine; global vaccine capacity-building; active learning; cGMP manufacturing
MeSH headings : Capacity Building / methods; Education, Continuing / methods; Humans; Influenza Vaccines / biosynthesis; Influenza Vaccines / immunology; Internet; Program Development / methods; Reproducibility of Results; Technology, Pharmaceutical / education; Technology, Pharmaceutical / methods; United States; United States Dept. of Health and Human Services; World Health Organization
TL;DR: The Biomanufacturing Training and Education Center at North Carolina State University has addressed the need for training for staff supporting operations within vaccine production facilities by developing and delivering a comprehensive training program, consisting of three courses: Fundamentals of cGMP Influenza Vaccine Manufacturing, Advanced Upstream Processes for Influenza vaccine Manufacturing, and Advanced Downstream processeses for influenza vaccine Manufacturing. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

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