@article{hwang_benson_2002, title={Biocontrol of Rhizoctonia stem and root rot of poinsettia with Burkholderia cepacia and binucleate Rhizoctonia}, volume={86}, ISSN={["0191-2917"]}, DOI={10.1094/PDIS.2002.86.1.47}, abstractNote={ Strategies for applying Burkholderia cepacia (strain 5.5B) and Pesta formulations of binucleate Rhizoctonia (BNR) isolates (BNR621 and P9023) were evaluated for biocontrol of Rhizoctonia stem and root rot of poinsettia caused by R. solani. During propagation, one application of B. cepacia suppressed stem rot, while application of either isolate of BNR did not. In contrast, after transplanting rooted poinsettias, one application of either BNR isolate was more effective for suppression of stem and root rot than application of B. cepacia. Sequential application of B. cepacia at propagation followed by a BNR isolate at transplanting was more effective over the crop production cycle than multiple applications of one biocontrol agent or combination application of both biocontrol agents. Root colonization by both biocontrol agents after transplanting rooted poinsettias was affected by application strategy. The least root colonization by both biocontrol agents occurred in the combination application. The highest root colonization by the BNR isolates was observed in the sequential application that provided the most effective disease control. Application of different biocontrol agents during the different production phases of poinsettia was effective for disease control, but understanding the interaction between biocontrol agents and root colonization was important to develop the best application strategy. }, number={1}, journal={PLANT DISEASE}, author={Hwang, J and Benson, DM}, year={2002}, month={Jan}, pages={47–53} } @article{huang_nelson_2001, title={Impact of pre-plant root substrate amendments on soilless substrate EC, pH, and nutrient availability}, volume={32}, ISSN={["0010-3624"]}, DOI={10.1081/CSS-120000968}, abstractNote={Pre-plant root substrate amendments have a great impact on the soluble salt level of plug seedling substrates. Nine common pre-plant amendments alone and a typical commercial combination of some of these showed that the greatest contributors to seedling substrate (2 sphagnum peat moss: 1 perlite: 1 vermiculite by volume) electrical conductivity (EC) level were gypsum, calcium nitrate, and potassium nitrate. Moderate contributions were realized from triple superphosphate, Epsom salt, and two commercial micronutrient mixes. The salt contributions from dolomitic limestone and wetting agent were small and of little commercial concern. At the most common application rates for seedling substrate, additions of gypsum, calcium nitrate, potassium nitrate, and the mixture raised the total substrate EC (saturated media extract procedure) by 1.47, 1.23, 1.09, and 1.57 dS m−1, respectively, from an initial no amendment level of 0.84 dS m−1. The resulting EC levels were unacceptable according to the current maximum standards. Seedling efficacy tests should be conducted with special attention paid to gypsum, calcium nitrate, and potassium nitrate for possible reduction or elimination from the pre-plant nutrient charge. During the 12 days following sowing, mean substrate EC for all amendments in the 0% leach treatment declined 19% while EC in the 20% leach treatment declined 36%. Of all the pre-plant amendments tested, the only single amendment that increased substrate pH over the control treatment was dolomitic limestone. All other amendments lowered substrate pH by 0.1 to 0.24 units when applied at standard commercial rates. When dolomitic limestone was incorporated in combination with other amendments in the mixture treatment, the substrate pH rise was not as great. The impacts of rate of nutrient amendments on nutrient availability are presented.}, number={17-18}, journal={COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS}, author={Huang, JS and Nelson, PV}, year={2001}, pages={2863–2875} } @book{huang_2001, title={Plant pathogenesis and resistance: Biochemistry and physiology of plant-microbe interactions}, ISBN={0792371186}, publisher={Dordrecht; Boston: Kluwer Academic Publishers}, author={Huang, J.-S.}, year={2001} } @article{huang_nelson_lee_2001, title={Seedling effect on root substrate pH}, volume={24}, ISSN={["0190-4167"]}, DOI={10.1081/PLN-100106972}, abstractNote={Paper used for seed germination tests may or may not contain limestone. The presence of limestone presented a pH buffer in this study that could interfere with studies addressing pH, calcium (Ca), or magnesium (Mg) effects on seedling development. Alternatively, pH of unbuffered chromatography paper was changed sufficiently by seedlings to confound research objectives that are pH dependent. Gradients across unbuffered substrate paper exceeded 1.5 pH units from a point under seedling roots to a distance 5 cm away. Precise measurements of effects of seedlings on substrate pH need to be taken in close proximity to the roots. Seedling effect on unlimed paper substrate pH varied across 25 taxa from a maximum rise of 1.4 units with zinnia to a decline of 1.1 units with tomato in the interim from sowing to separation of cotyledons. In general, pH levels in paper were higher when a basic complete nutrient solution with all nitrogen (N) in NO3 form was supplied compared to an acidic solution with N comprised of 40% NH4 + 60% NO3. In a peat moss:perlite substrate, 11 taxa altered substrate pH over a range of 0.6 units, much lower than on paper. While five taxa had similar pH enhancing or suppressing influences in the paper and peat moss: perlite substrates, six shifted the direction of the pH change. The effects of most taxa on pH were similar when fertilized with acidic or basic fertilizers. Exceptions included pansy (Viola x wittrockiana Gams.), petunia (Petunia x hybrida Vilm.-Andr.), snapdragon (Antirrhinum majus L.), and verbena (Veberna x hybrida Voss) that responded to basic fertilizer with higher substrate pH rises than anticipated. Only modest shifts occurred in the order of taxa in terms of their effects on substrate pH over time in the peat moss:perlite substrate. Taxa that tended to raise peat moss:perlite substrate pH regardless of fertilizer type or time included pansy, petunia, and vinca (Catharanthus roseus G. Don) while those that lowered pH were celosia (Celosia cristata L.), tomato (Lycopersicon esculentum Mill.), and zinnia (Zinnia elegans Jacq.).}, number={8}, journal={JOURNAL OF PLANT NUTRITION}, author={Huang, JS and Nelson, PV and Lee, JW}, year={2001}, pages={1133–1147} } @article{heilmann_shin_huang_perera_davies_2001, title={Transient dissociation of polyribosomes and concurrent recruitment of calreticulin and calmodulin transcripts in gravistimulated maize pulvini}, volume={127}, ISSN={["1532-2548"]}, DOI={10.1104/pp.127.3.1193}, abstractNote={In plants, sugars are the main respiratory substrates and important signaling molecules in the regulation of carbon metabolism. Sugar signaling studies suggested that sugar sensing involves several key components, among them hexokinase (HXK). Although the sensing mechanism of HXK is unknown, several experiments support the hypothesis that hexose phosphorylation is a determining factor. Glucose (Glc) analogs transported into cells but not phosphorylated are frequently used to test this hypothesis, among them 3-O-methyl-Glc (3-OMG). The aim of the present work was to investigate the effects and fate of 3-OMG in heterotrophic plant cells. Measurements of respiration rates, protein and metabolite contents, and protease activities and amounts showed that 3-OMG is not a respiratory substrate and does not contribute to biosynthesis. Proteolysis and lipolysis are induced in 3-OMG-fed maize (Zea mays L. cv DEA) roots in the same way as in sugar-starved organs. However, contrary to the generally accepted idea, phosphorous and carbon nuclear magnetic resonance experiments and enzymatic assays prove that 3-OMG is phosphorylated to 3-OMG-6-phosphate, which accumulates in the cells. Insofar as plant HXK is involved in sugar sensing, these findings are discussed on the basis of the kinetic properties because the catalytic efficiency of HXK isolated from maize root tips is five orders of magnitude lower for 3-OMG than for Glc and Man.}, number={3}, journal={PLANT PHYSIOLOGY}, author={Heilmann, I and Shin, J and Huang, J and Perera, IY and Davies, E}, year={2001}, month={Nov}, pages={1193–1203} } @article{saleh_huang_huang_1997, title={Bacillus pumilus, the cause of bacterial blotch of immature Balady peach in Egypt}, volume={145}, ISSN={["0931-1785"]}, DOI={10.1111/j.1439-0434.1997.tb00348.x}, abstractNote={AbstractFive pectolytic isolates of Bacillus were isolated from bacterial blotch of immature fruits and leaves of Balady peach (Prunus persica cv. Balady) grown in EI‐Minia Governorate in Egypt. Inoculation of the isolated bacteria into premature peach fruits and leaves resulted in the development of large brown spots. The isolated bacterium was identified as Bacillus pumilus from its physiological and biochemical properties, pathogenicity, electron microscopy and fatty acid composition analysis. B. pumilus also induced soft rot on wounded American stone and pome fruits such as Autumn Large and Southern peach, Large Red plums, Golden Delicious and Gala apple and Bartlet pear. Unwounded Autumn Large peach and Bartlet pear were also affected. Moreover, they degraded the parenchymatous tissues of many tested plant organs under experimental conditions. B. pumilus produced endopolygalacturonase (EC 4.2.2.1) and endopectin lyase (EC4.2.2.3) in vitro and in vivo. This is the first report of the disease occurring in Egypt.}, number={10}, journal={JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT}, author={Saleh, OI and Huang, PY and Huang, JS}, year={1997}, month={Oct}, pages={447–453} } @article{huang_barker_1991, title={GLYCEOLLIN-I IN SOYBEAN-CYST NEMATODE INTERACTIONS - SPATIAL AND TEMPORAL DISTRIBUTION IN ROOTS OF RESISTANT AND SUSCEPTIBLE SOYBEANS}, volume={96}, ISSN={["0032-0889"]}, DOI={10.1104/pp.96.4.1302}, abstractNote={Accumulation of the phytoalexin glyceollin I in roots of soybean (Glycine max [L.] Merr.) following inoculation with race 1 of Heterodera glycines Ichinohe, the soybean cyst nematode (SCN), was determined in a whole-root system by high performance liquid chromatography (HPLC) and in a cross-section system by a radioimmunoassay procedure. In the whole-root system, roots were harvested from controls and nematode-inoculated seedlings immediately after inoculation and at 2-day intervals for 8 days. The roots were extracted with ethanol, and the extracts were subjected to HPLC. Glyceollin I was not detected in roots of either resistant cultivar Centennial or susceptible cultivar Ransom immediately after inoculation with SCN but steadily accumulated in large quantity in roots of Centennial. Accumulation of glyceollin I in roots of Ransom following nematode inoculation was minimal. In the cross-section system, 3-day-old soybean seedlings were inoculated with juvenile nematodes, and root segments containing a single nematode were dissected from inoculated plants at 4-hour intervals under a dissecting microscope. The root segments were embedded in ice and cut into 16-micrometer sections with a cryostat microtome. The spatial and temporal distribution of glyceollin I was determined with a radioimmunoassay procedure specific for the phytoalexin. Glyceollin I was found to accumulate in tissues immediately adjacent to the head region of the nematode in Centennial but not in Ransom. Glyceollin I was detected 8 hours after nematode penetration, and the concentration increased steadily up to 0.3 micromole per milliliter in Centennial 24 hours after penetration.}, number={4}, journal={PLANT PHYSIOLOGY}, author={HUANG, JS and BARKER, KR}, year={1991}, month={Aug}, pages={1302–1307} }