@article{nierobisz_sporer_strasburg_reed_velleman_ashwell_felts_mozdziak_2012, title={Differential expression of genes characterizing myofibre phenotype}, volume={43}, ISSN={["1365-2052"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84859745099&partnerID=MN8TOARS}, DOI={10.1111/j.1365-2052.2011.02249.x}, abstractNote={Summary}, number={3}, journal={ANIMAL GENETICS}, author={Nierobisz, L. S. and Sporer, K. R. B. and Strasburg, G. M. and Reed, K. M. and Velleman, S. G. and Ashwell, C. M. and Felts, J. V. and Mozdziak, P. E.}, year={2012}, month={Jun}, pages={298–308} }
 @article{nierobisz_hentz_felts_mozdziak_2010, title={Fiber Phenotype and Coenzyme Q(10) Content in Turkey Skeletal Muscles}, volume={192}, ISSN={["1422-6421"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-78649321430&partnerID=MN8TOARS}, DOI={10.1159/000319550}, abstractNote={Phenotypical differences between muscle fibers are associated with a source of cellular energy. Coenzyme Q<sub>10</sub> (CoQ<sub>10</sub>) is a major component of the mitochondrial oxidative phosphorylation process, and it significantly contributes to the production of cellular energy in the form of ATP. The objective of this study was to determine the relationship between whole-tissue CoQ<sub>10</sub> content, mitochondrial CoQ<sub>10</sub> content, mitochondrial protein, and muscle phenotype in turkeys. Four specialized muscles (anterior latissimus dorsi, ALD; posterior latissimus dorsi, PLD; pectoralis major, PM, and biceps femoris, BF) were evaluated in 9- and 20-week-old turkey toms. The amount of muscle mitochondrial protein was determined using the Bradford assay and CoQ<sub>10</sub> content was measured using HPLC-UV. The amount of mitochondrial protein relative to total protein was significantly lower (p < 0.05) at 9 compared to 20 weeks of age. All ALD fibers stained positive for anti-slow (S35) MyHC antibody. The PLD and PM muscle fibers revealed no staining for slow myosin heavy chain (S35 MyHC), whereas half of BF muscle fibers exhibited staining for S35 MyHC at 9 weeks and 70% at 20 weeks of age. The succinate dehydrogenase (SDH) staining data revealed that SDH significantly increases (p < 0.05) in ALD and BF muscles and significantly decreases (p < 0.05) in PLD and PM muscles with age. The study reveals age-related decreases in mitochondrial CoQ<sub>10</sub> content in muscles with fast/glycolytic profile, and demonstrates that muscles with a slow/oxidative phenotypic profile contain a higher proportion of CoQ<sub>10</sub> than muscles with a fast/glycolytic phenotypic profile.}, number={6}, journal={CELLS TISSUES ORGANS}, author={Nierobisz, L. S. and Hentz, N. G. and Felts, J. V. and Mozdziak, P. E.}, year={2010}, pages={382–394} }
 @article{nierobisz_felts_mozdziak_2009, title={Apoptosis and macrophage infiltration occur simultaneously and present a potential sign of muscle injury in skeletal muscle of nutritionally compromised, early post-hatch turkeys}, volume={153}, ISSN={["1879-1107"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-63249099336&partnerID=MN8TOARS}, DOI={10.1016/j.cbpb.2009.01.015}, abstractNote={Physical stress and malnutrition may cause elimination of myonuclei and produce inflammatory response in muscle. The objective of this study was to histochemically determine the association of apoptosis and/or macrophage infiltration with changes in muscle satellite cell mitotic activity in pectoralis thoracicus muscle of early post-hatch turkey toms. Feed-deprived birds and birds provided with three different levels of crude protein and amino acids (0.88 NRC, 1.00 NRC, and 1.12 NRC) were used in this model. The number of apoptotic nuclei was significantly elevated (P<0.05) and presence of macrophage infiltration was readily detectable in feed-deprived and 0.88 NRC treatment groups 72 h and 96 h post-hatch suggesting potential muscle injury and/or muscle remodeling. The number of apoptotic nuclei was the same (P>0.05), and there was no detectable macrophage infiltration present in birds placed on 1.00 NRC and 1.12 NRC diet 72 h, 96 h, and 120 h post-hatch. At 120 h post-hatch, feed-deprived and 0.88 NRC birds were characterized by no detectable levels of macrophage infiltration and a significant drop (P<0.05) in apoptotic nuclei. Understanding mechanisms that correlate early nutrition with skeletal muscle growth and development may present a useful tool in optimizing muscle health and improving meat quality and yield.}, number={1}, journal={COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY}, author={Nierobisz, L. S. and Felts, J. V. and Mozdziak, P. E.}, year={2009}, month={May}, pages={61–65} }
 @article{ferket_oviedo-rondon_mente_bohorquez_santos_grimes_richards_dibner_felts_2009, title={Organic trace minerals and 25-hydroxycholecalciferol affect performance characteristics, leg abnormalities, and biomechanical properties of leg bones of turkeys}, volume={88}, ISSN={["1525-3171"]}, DOI={10.3382/ps.2008-00200}, abstractNote={Leg problems and resulting mortality can exceed 1% per week in turkey toms starting at approximately 15 wk of age. Dietary supplementation of organic trace minerals (MIN) and 25-hydroxycholecalciferol (HyD) may improve performance, decrease incidence of leg abnormalities, and increase bone strength. Nicholas 85X700 toms were assigned to 4 treatments consisting of a factorial arrangement of 2 concentrations of MIN (0 and 0.1% of Mintrex P(Se), which adds 40, 40, 20, and 0.3 mg/kg of Zn, Mn, Cu, and Se, respectively) and 2 concentrations of HyD (0 and 92 microg/kg of HyD). Diets were formulated to be equal in nutrient content and fed ad libitum as 8 feed phases. Feed intake and BW were measured at 6, 12, 15, 17, and 20 wk of age. Valgus, varus, and shaky leg defects were determined at 12, 15, 17, and 20 wk of age. Tibia and femur biomechanical properties were evaluated by torsion and bending tests at 17 wk of age. There were no treatment effects on BW. Only MIN significantly improved feed conversion ratio through to 20 wk of age. Cumulative mortality at 3 wk of age was greater among the MIN birds, but it was lower by 20 wk (P = 0.085). The MIN decreased the incidence of varus defects at 17 wk of age; shaky leg at 12, 15, and 17 wk of age; and valgus defects at 15, 17, and 20 wk of age. There were no MIN x HyD interaction effects on individual gait problems. Maximum load and the bending stress required for tibias to break in a 4-point assay were increased with MIN supplementation, especially when HyD was also added. Maximum shear stress at failure of femoral bones in a torsion assay was increased by supplementation with both MIN and HyD together. Dietary supplementation of MIN and HyD may improve biomechanical properties of bones. Dietary MIN supplementation may improve feed conversion of turkeys, likely by decreasing leg problems.}, number={1}, journal={POULTRY SCIENCE}, author={Ferket, P. R. and Oviedo-Rondon, E. O. and Mente, P. L. and Bohorquez, D. V. and Santos, A. A., Jr. and Grimes, J. L. and Richards, J. D. and Dibner, J. J. and Felts, V.}, year={2009}, month={Jan}, pages={118–131} }
 @article{nierobisz_felts_mozdziak_2007, title={The effect of early dietary amino acid levels on muscle satellite cell dynamics in turkeys}, volume={148}, ISSN={["1879-1107"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-35448939699&partnerID=MN8TOARS}, DOI={10.1016/j.cbpb.2007.06.006}, abstractNote={Understanding the relationship between nutrition and satellite cell activity will be beneficial in obtaining optimal muscle growth and meat production. The objective of this study was to evaluate the effect of early post-hatch levels of dietary amino acids ± 0.88 NRC, 1.00 NRC, and 1.12 NRC), and feed deprivation on the satellite cell mitotic activity, pectoralis thoracicus muscle weight, and body weight of male turkeys (Meleagris gallopavo). Birds from each treatment were injected with 5-bromo-2′-deoxyuridine (BrdU) to label mitotically active cells. The right pectoralis thoracicus was harvested 1 h after BrdU injection for immunohistochemical and myofiber diameter analysis. On the third day post-hatch, satellite cell mitotic activity was the highest (P < 0.05) in the 0.88 NRC amino acid treatment group and the lowest (P < 0.05) in the feed-deprived group. On the fourth day post-hatch, feed-deprived birds exhibited the lowest (P < 0.05) satellite cell mitotic activity and muscle weight. At 140 days of age, there were no significant differences (P > 0.05) between treatments in body weight or pectoralis thoracicus muscle weight. Research evaluating species-related differences in apoptotic events and in genes regulating cell proliferation may be necessary to devise feeding strategies aimed at obtaining optimal pectoralis thoracicus muscle yield at market age.}, number={3}, journal={COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY}, author={Nierobisz, L. S. and Felts, V. and Mozdziak, P. E.}, year={2007}, month={Nov}, pages={286–294} }