Kaitlyn Bacon

Chemical & Biomolecular Engr

Works (8)

Updated: July 19th, 2023 21:17

2021 journal article

Quantitative Yeast-Yeast Two Hybrid for the Discovery and Binding Affinity Estimation of Protein-Protein Interactions

ACS SYNTHETIC BIOLOGY, 10(3), 505–514.

By: K. Bacon n, A. Blain n, J. Bowen n, M. Burroughs n, N. McArthur  n, S. Menegatti n , B. Rao n

author keywords: yeast two-hybrid; protein-protein interactions; protein engineering; yeast surface display; post-translational modification; cDNA library
MeSH headings : Gene Library; Genes, Reporter; Protein Binding; Protein Domains; Protein Interaction Maps; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Smad3 Protein / chemistry; Smad3 Protein / metabolism; Transcription Factors / chemistry; Transcription Factors / metabolism; Two-Hybrid System Techniques
TL;DR: A quantitative yeast-yeast two-hybrid system that not only enables the discovery of specific protein-protein interactions but also efficient, quantitative estimation of their binding affinities (KD), and it was shown that qYY2H could also quantitatively evaluate binding interactions mediated by post-translational modifications on the bait protein. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: March 29, 2021

2021 journal article

Screening of Yeast Display Libraries of Enzymatically Treated Peptides to Discover Macrocyclic Peptide Ligands

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(4).

By: J. Bowen n, J. Schneible n, K. Bacon n, C. Labar n, S. Menegatti n  & B. Rao n

author keywords: yeast display library; cyclic peptide; Yes Associated Protein (YAP) 65; WW domain; transglutaminase
MeSH headings : Adaptor Proteins, Signal Transducing / chemistry; Adaptor Proteins, Signal Transducing / metabolism; Albumins / metabolism; Binding Sites; Combinatorial Chemistry Techniques; Endoplasmic Reticulum / metabolism; Flow Cytometry; Ligands; Muramidase / metabolism; Peptides, Cyclic / isolation & purification; Peptides, Cyclic / pharmacology; Protein Binding; Protein Engineering / methods; Transcription Factors / chemistry; Transcription Factors / metabolism; Transglutaminases / metabolism; YAP-Signaling Proteins; Yeasts / genetics; Yeasts / growth & development
TL;DR: The usefulness of yeast surface display for screening transglutaminase-cyclized peptide libraries, and efficient identification of cyclic peptide ligands, are demonstrated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: March 22, 2021

2020 journal article

Isolation of Chemically Cyclized Peptide Binders Using Yeast Surface Display

ACS COMBINATORIAL SCIENCE, 22(10), 519–532.

By: K. Bacon n, A. Blain n, M. Burroughs n, N. McArthrur, B. Rao n & S. Menegatti n 

author keywords: cyclic peptides; interleukin-17 (IL-17); library screening; yeast-display libraries; affinity ligands
MeSH headings : Biophysical Phenomena; Cell Membrane / chemistry; Cyclization; Flow Cytometry; Humans; Interleukin-17 / chemistry; Molecular Docking Simulation; Peptide Library; Peptides, Cyclic / isolation & purification; Protein Binding; Protein Conformation; Protein Engineering; Recombinant Proteins / chemistry; Saccharomyces cerevisiae / chemistry; Saccharomyces cerevisiae / ultrastructure; Structure-Activity Relationship; Surface Properties
TL;DR: The results show that yeast surface display can be used to efficiently isolate and characterize cyclic peptides generated by chemical modification from combinatorial libraries. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: November 9, 2020

2020 review

Past, Present, and Future of Affinity-based Cell Separation Technologies

[Review of ]. ACTA BIOMATERIALIA, 112, 29–51.

By: K. Bacon n, A. Lavoie n, B. Rao n, M. Daniele n & S. Menegatti n 

author keywords: Cell purification; Immunoaffinity; MACS; FACS; Microfluidics
MeSH headings : Cell Separation; Chromatography, Affinity; Flow Cytometry; Ligands; Regenerative Medicine
TL;DR: This review discusses past and current methods for developing cell-targeting affinity ligands and their application in cell purification, along with the benefits and challenges associated with different purification formats, and presents new technologies towards improving the viability and throughput of cell products for tissue engineering and regenerative medicine. (via Semantic Scholar)
UN Sustainable Development Goal Categories
9. Industry, Innovation and Infrastructure (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 3, 2020

2020 journal article

Peptides and pseudopeptide ligands: a powerful toolbox for the affinity purification of current and next-generation biotherapeutics

JOURNAL OF CHROMATOGRAPHY A, 1635.

By: W. Chu n, R. Prodromou n, K. Day n, J. Schneible n, K. Bacon n, J. Bowen n, R. Kilgore n, C. Catella n ...

author keywords: Affinity ligands; peptides; peptoids; pseudopeptides; biotherapeutics
MeSH headings : Antibodies / isolation & purification; Biological Products / isolation & purification; Chemistry, Pharmaceutical / methods; Chromatography, Affinity; Family Characteristics; Humans; Ligands; Peptides / isolation & purification; Peptoids / chemistry; Proteins / isolation & purification
TL;DR: This work presents a comprehensive and systematic review of the literature on peptide-based ligands and their use in the affinity purification of established and upcoming biological drugs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: January 25, 2021

2020 journal article

Use of Target-Displaying Magnetized Yeast in Screening mRNA-Display Peptide Libraries to Identify Ligands

ACS COMBINATORIAL SCIENCE, 22(12), 738–744.

By: K. Bacon n, J. Bowen n, H. Reese n, B. Rao n & S. Menegatti n 

author keywords: yeast surface display; yeast magnetization; peptide ligands; mRNA-display library; ligand discovery
MeSH headings : Ligands; Mitochondrial Membrane Transport Proteins / chemistry; Models, Molecular; Peptide Library; RNA, Messenger / chemistry; Saccharomyces cerevisiae / chemistry; Saccharomyces cerevisiae Proteins / chemistry; Transcription Factors / chemistry
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: January 11, 2021

2019 journal article

Screening Yeast Display Libraries against Magnetized Yeast Cell Targets Enables Efficient Isolation of Membrane Protein Binders

ACS COMBINATORIAL SCIENCE, 21(12), 817–832.

By: K. Bacon n, M. Burroughs n, A. Blain n, S. Menegatti n  & B. Rao n

author keywords: yeast magnetization; combinatorial library screening; ligand discovery; yeast surface display; membrane protein; Sso7d; nanobody
MeSH headings : Combinatorial Chemistry Techniques; Drug Evaluation, Preclinical; Ferric Compounds / chemistry; Ferric Compounds / pharmacology; Magnetic Phenomena; Mitochondria / drug effects; Mitochondria / metabolism; Mitochondrial Membrane Transport Proteins / antagonists & inhibitors; Mitochondrial Membrane Transport Proteins / isolation & purification; Mitochondrial Membrane Transport Proteins / metabolism; Nanoparticles / chemistry; Protein Binding / drug effects; Saccharomyces cerevisiae / cytology; Saccharomyces cerevisiae / drug effects; Saccharomyces cerevisiae / metabolism; Saccharomyces cerevisiae Proteins / antagonists & inhibitors; Saccharomyces cerevisiae Proteins / isolation & purification; Saccharomyces cerevisiae Proteins / metabolism; Small Molecule Libraries / chemistry; Small Molecule Libraries / pharmacology
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: December 30, 2019

2019 article

Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces cerevisiae Using Inefficient Ribosomal Skipping

GENOTYPE PHENOTYPE COUPLING: METHODS AND PROTOCOLS, Vol. 2070, pp. 321–334.

By: C. Cruz-Teran n, K. Bacon n & B. Rao n

author keywords: Yeast surface display; Self-cleaving peptides; 2A peptides; Secretion; Ribosomal skipping; Sso7d
MeSH headings : Peptide Library; Peptides / metabolism; Protein Biosynthesis; Recombinant Proteins / biosynthesis; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Ribosomes / metabolism; Saccharomyces cerevisiae / chemistry; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism
TL;DR: This work has developed a platform that exploits inefficient ribosomal skipping by the F2A peptide for simultaneous soluble secretion and cell surface display of protein in the yeast Saccharomyces cerevisiae and provides specific recommendations for the purification of the secreted protein. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: September 7, 2020

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