Works (15)
Updated: July 5th, 2023 16:04
2013 journal article
The red clover necrotic mosaic virus capsid protein N-terminal amino acids possess specific RNA binding activity and are required for stable virion assembly
VIRUS RESEARCH, 176(1-2), 107–118.
author keywords: RCNMV; Virus assembly; RNA-CP interactions; TABS; TA; Co-packaging
MeSH headings : Capsid Proteins / genetics; Capsid Proteins / metabolism; DNA Mutational Analysis; RNA-Binding Proteins / genetics; RNA-Binding Proteins / metabolism; Sequence Deletion; Tombusviridae / genetics; Tombusviridae / physiology; Virion / metabolism; Virus Assembly
TL;DR:
The N-terminal region of the CP is involved in both producing two virion populations due to its RNA binding properties and virion stability and was indispensable for stable virion formation and the region spanning CP residues 5-15 is required for systemic movement.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2012 journal article
The Red clover necrotic mosaic virus capsid protein N-terminal lysine-rich motif is a determinant of symptomatology and virion accumulation
MOLECULAR PLANT PATHOLOGY, 13(7), 744–754.
MeSH headings : Alanine / genetics; Amino Acid Motifs; Amino Acid Sequence; Amino Acid Substitution / genetics; Capsid Proteins / chemistry; Capsid Proteins / metabolism; Genome, Viral / genetics; Lysine / metabolism; Molecular Sequence Data; Mutation / genetics; Plant Diseases / virology; Plant Viral Movement Proteins / metabolism; RNA, Viral / metabolism; Sequence Alignment; Structure-Activity Relationship; Tobacco / virology; Tombusviridae / genetics; Tombusviridae / metabolism; Tombusviridae / physiology; Tombusviridae / ultrastructure; Virion / metabolism; Virion / ultrastructure
TL;DR:
Observations reaffirm the necessity of the N-terminal lysine-rich residues of the RCNMV CP for efficient virion accumulation and reveal additional roles for the CP in the modulation of host symptomatology, independent of its role in virion assembly and the rate of systemic viral movement in N. benthamiana.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2008 journal article
A versatile assay for the identification of RNA silencing suppressors based on complementation of viral movement
MOLECULAR PLANT-MICROBE INTERACTIONS, 21(7), 879–890.
author keywords: IVIS lumina; p8; p9; pPZP212; VSR
MeSH headings : Agrobacterium tumefaciens / genetics; Arabidopsis / genetics; Arabidopsis / virology; Base Sequence; Capsid Proteins / genetics; Capsid Proteins / physiology; Carmovirus / genetics; Carmovirus / pathogenicity; Carmovirus / physiology; DNA, Viral / genetics; Genes, Reporter; Genetic Complementation Test; Green Fluorescent Proteins / genetics; Host-Pathogen Interactions / genetics; Movement; Plant Diseases / genetics; Plant Diseases / virology; RNA Interference; RNA, Viral / genetics; Recombinant Proteins / genetics; Suppression, Genetic; Tobacco / genetics; Tobacco / virology
TL;DR:
While the TCV-sGFP construct is primarily used as an infectious RNA transcript, it was also subcloned for direct expression from Agrobacterium tumefaciens for simple quantification of suppressor activity based on fluorescence levels in whole leaves, providing the flexibility to assay for suppressorActivity in either the cytoplasm or nucleus, depending on the construct employed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2008 journal article
RNA-RNA interactions between RNA elements at the 5 ' end and at the upstream of sgRNA of RNA genome are required for Potato virus X RNA replication
PLANT PATHOLOGY JOURNAL, 24(3), 289–295.
author keywords: plus-strand RNA accumulation; PVX; replication; RNA-RNA base-pairing
TL;DR:
Results indicate that the RNA element located at the 5` end of PVX genome involved in RNA-RNA interactions and play a key role in high-level accumulation of plus-strand RNA in vivo.
(via Semantic Scholar)
www.ndsl.kr (publisher PDF)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2008 journal article
The Red clover necrotic mosaic virus RNA-2 encoded movement protein is a second suppressor of RNA silencing
VIROLOGY, 381(2), 277–286.
author keywords: TCV-sGFP; Red clover necrotic mosaic virus; RCNMV; Movement protein; MP; VSR; RNA silencing
MeSH headings : Carmovirus / genetics; Carmovirus / metabolism; Genetic Complementation Test; Green Fluorescent Proteins / metabolism; Plant Viral Movement Proteins / metabolism; RNA Interference; RNA, Viral / genetics; RNA, Viral / metabolism; Tombusviridae / genetics; Tombusviridae / metabolism
TL;DR:
A newly developed viral-based assay for the identification of silencing suppression activity was used to provide evidence for a second, mechanistically distinct method ofsilencing suppression provided for by the RCNMV movement protein (MP).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2005 journal article
cis-Acting sequences required for coat protein binding and in vitro assembly of Potato virus X
VIROLOGY, 334(1), 83–97.
author keywords: PVX; assembly; OAS; SELEX; stem-loop structure
MeSH headings : 5' Untranslated Regions; Base Sequence; Binding Sites / genetics; Capsid Proteins / genetics; Capsid Proteins / isolation & purification; Capsid Proteins / metabolism; DNA, Viral / genetics; Escherichia coli / genetics; Genetic Complementation Test; Microscopy, Electron; Molecular Sequence Data; Nucleic Acid Conformation; Potexvirus / genetics; Potexvirus / physiology; Potexvirus / ultrastructure; Protein Binding; RNA, Viral / chemistry; RNA, Viral / genetics; Recombinant Proteins / genetics; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Virus Assembly / genetics
TL;DR:
Results suggest that RNA secondary structural elements within SL1 and/or sequences therein are crucial for formation of VLPs and are required for the specific recognition by the CP subunit.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2003 journal article
Cis-acting regulatory elements in the potato virus X 3 ' non-translated region differentially affect minus-strand and plus-strand RNA accumulation
JOURNAL OF MOLECULAR BIOLOGY, 326(3), 701–720.
author keywords: potato virus X; RNA replication; RNA structure; RNA virus; stem-loop
MeSH headings : 3' Untranslated Regions; Base Sequence; Molecular Sequence Data; Nucleic Acid Conformation; Potexvirus / genetics; RNA, Viral / chemistry; RNA, Viral / metabolism; Regulatory Sequences, Nucleic Acid; Sequence Homology, Nucleic Acid
TL;DR:
PVX 3′ NTR contains multiple, overlapping elements that influence accumulation of both minus and plus-strand RNA, and potential polyadenylation signals (near upstream elements; NUEs) in the 3″ NTR of PVX RNA were more important for plus-Strand RNA accumulation than for minus-strander RNA accumulation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2000 journal article
Soluble, template-dependent extracts from Nicotiana benthamiana plants infected with potato virus X transcribe both plus- and minus-strand RNA templates
VIROLOGY, 275(2), 444–451.
MeSH headings : Electrophoresis, Agar Gel; Plant Extracts / metabolism; Plants, Toxic; Potexvirus / metabolism; Potexvirus / physiology; RNA, Viral / analysis; RNA, Viral / biosynthesis; RNA-Dependent RNA Polymerase / metabolism; Tobacco / virology; Transcription, Genetic; Virus Replication
TL;DR:
A method to convert membrane-bound replication complexes isolated from Nicotiana benthamiana plants infected with potato virus X to a soluble, template-dependent system for analysis of RNA synthesis has been developed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1999 journal article
Long-distance RNA-RNA interactions and conserved sequence elements affect potato virus X plus-strand RNA accumulation
RNA, 5(5), 636–645.
author keywords: cis-acting regulatory elements; plant RNA virus; RNA replication; subgenomic RNA synthesis
MeSH headings : 5' Untranslated Regions / genetics; Macromolecular Substances; Mutagenesis, Site-Directed; Plants, Toxic; Potexvirus / genetics; Potexvirus / physiology; Protoplasts / virology; RNA, Viral / chemistry; RNA, Viral / metabolism; Regulatory Sequences, Nucleic Acid; Templates, Genetic; Tobacco / cytology; Tobacco / virology; Transcription, Genetic; Virulence / genetics; Virus Replication
TL;DR:
It is indicated that long distance RNA-RNA interactions and the sequences of the interacting elements are required for PVX plus-strand RNA accumulation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1999 journal article
Recent development in detection and identification of fruit tree viruses
Plant Pathology Journal, 15(4), 199.
Source: NC State University Libraries
Added: August 6, 2018
1999 journal article
Restoration of a stem-loop structure required for potato virus X RNA accumulation indicates selection for a mismatch and a GNRA tetraloop
VIROLOGY, 260(2), 342–353.
MeSH headings : Adenosine / genetics; Base Pair Mismatch / genetics; Base Pairing / genetics; Base Sequence; Gene Expression Regulation, Viral; Genome, Viral; Mutagenesis, Site-Directed; Mutation / genetics; Nucleic Acid Conformation; Phenotype; Plants, Toxic; Potexvirus / genetics; Potexvirus / growth & development; Protoplasts / virology; RNA, Messenger / chemistry; RNA, Messenger / genetics; RNA, Messenger / metabolism; RNA, Viral / biosynthesis; RNA, Viral / chemistry; RNA, Viral / genetics; RNA, Viral / metabolism; Thermodynamics; Tobacco / cytology; Tobacco / virology; Virus Replication / genetics
TL;DR:
Data indicate that important sequence and secondary structural elements within SL1 are required for efficient viral infection and that multiple A insertions within the TL and loop C regions, potentially by polymerase stuttering, accompany restoration of SL1 structure.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018
1998 journal article
Sequence element required for efficient -1 ribosomal frameshifting in red clover necrotic mosaic dianthovirus
VIROLOGY, 250(1), 50–59.
MeSH headings : Amino Acid Sequence; Base Pairing; Base Sequence; Cells, Cultured; Fabaceae / virology; Frameshifting, Ribosomal / genetics; Molecular Sequence Data; Mosaic Viruses / genetics; Mutagenesis, Site-Directed; Nucleic Acid Conformation; Plants, Medicinal; Plants, Toxic; Protoplasts / virology; RNA, Messenger / chemistry; RNA, Viral / chemistry; RNA, Viral / genetics; Recombinant Fusion Proteins; Tobacco / virology
TL;DR:
The essential role of a predicted secondary structure that does not involve a pseudoknot in the expression of the RCNMV polymerase by ribosomal frameshifting is demonstrated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1998 journal article
Stem-loop structure in the 5 ' region of potato virus X genome required for plus-strand RNA accumulation
JOURNAL OF MOLECULAR BIOLOGY, 284(3), 591–608.
author keywords: RNA structure; RNA virus; RNA replication; potato virus X; stem-loop
MeSH headings : Base Sequence; Genome, Viral; Molecular Sequence Data; Mutagenesis, Site-Directed; Nucleic Acid Conformation; Plants, Toxic; Potexvirus / genetics; Protoplasts / virology; RNA, Viral / chemistry; RNA, Viral / metabolism; Thermodynamics; Tobacco / metabolism; Tobacco / virology
TL;DR:
Data indicate that multiple features of SL1 are critical for accumulation of PVX plus-strand RNA, including a UUCG tetraloop, indicating that the sequence of this element was important beyond just providing stabilization of the structure.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1997 journal article
Mutations that alter a conserved element upstream of the potato virus X triple block and coat protein genes affect subgenomic RNA accumulation
VIROLOGY, 232(1), 187–197.
MeSH headings : Base Sequence; Capsid / genetics; Conserved Sequence; Genome, Viral; Kinetics; Molecular Sequence Data; Mutation; Potexvirus / genetics; Protoplasts / metabolism; RNA, Messenger / genetics; RNA, Viral / genetics; RNA, Viral / metabolism
TL;DR:
Studies involving inoculation of tobacco plants with the modified transcripts suggested that the conserved octanucleotide element functions in sgRNA accumulation and some other aspect of the infection process.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1996 journal article
The 5' nontranslated region of potato virus X RNA affects both genomic and subgenomic RNA synthesis
Journal of Virology, 70(8), 5533.
Source: NC State University Libraries
Added: August 6, 2018
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