Works (13)
Updated: July 20th, 2023 21:17
2019 journal article
Outcomes and characterization of chromosomal self-targeting by native CRISPR-Cas systems in Streptococcus thermophilus
FEMS MICROBIOLOGY LETTERS, 366(9).
author keywords: CRISPR; lactic acid bacteria; Cas9; Streptococcus; genome editing
MeSH headings : Bacteriophages / genetics; CRISPR-Cas Systems; Chromosomes, Bacterial / genetics; Gene Editing; Genome, Bacterial; Homologous Recombination; Plasmids / genetics; Streptococcus thermophilus / genetics; Streptococcus thermophilus / virology
TL;DR:
Results suggest that HR contributes to the plasticity and remodeling of bacterial genomes, leading to evasion of genome targeting by CRISPR-Cas systems.
(via Semantic Scholar)
academic.oup.com (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: September 3, 2019
2019 journal article
Short communication: Transcriptional response to a large genomic island deletion in the dairy starter culture Streptococcus thermophilus
JOURNAL OF DAIRY SCIENCE, 102(9), 7800–7806.
author keywords: fermentation; Streptococcus thermophilus; transcriptome; CRISPR
MeSH headings : Animals; Clustered Regularly Interspaced Short Palindromic Repeats; Fermentation; Gene Deletion; Gene Expression Regulation, Bacterial / physiology; Genomic Islands; Genomics; Lactic Acid / metabolism; Lactobacillus delbrueckii / metabolism; Milk / microbiology; Streptococcus thermophilus / genetics; Streptococcus thermophilus / metabolism; Transcriptome; Yogurt / microbiology
TL;DR:
Results suggest that deletion of a 102-kb genomic island in Strep.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 26, 2019
2017 journal article
Deletion of Lipoteichoic Acid Synthase Impacts Expression of Genes Encoding Cell Surface Proteins in Lactobacillus acidophilus
Frontiers in Microbiology, 8.
TL;DR:
Results elucidate the impact of ltaS deletion on the transcriptome and extracellular proteins of L. acidophilus, suggesting roles of LTA in cell morphology and ion homeostasis as a structural component of the Gram positive cell wall.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: NC State University Libraries, ORCID, NC State University Libraries
Added: August 6, 2018
2015 journal article
CRISPR-based screening of genomic island excision events in bacteria
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 112(26), 8076–8081.
author keywords: CRISPR; lactic acid bacteria; transposons; IS-elements; Cas9
MeSH headings : Clustered Regularly Interspaced Short Palindromic Repeats; Gene Deletion; Genome, Bacterial; Homeostasis; Homologous Recombination; Streptococcus thermophilus / genetics; Streptococcus thermophilus / growth & development
TL;DR:
This study shows that CRISPR-Cas systems can be directed to target and delete genomic islands that are flanked by insertion-sequence elements and devoid of essential genes, and established that self-targeting CRISpr-cas systems may direct significant evolution of bacterial genomes on a population level, influencing genome homeostasis and remodeling.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2015 review
Harnessing CRISPR-Cas systems for bacterial genome editing
[Review of ]. TRENDS IN MICROBIOLOGY, 23(4), 225–232.
author keywords: genome editing; CRISPR-Cas; Cas9
MeSH headings : Bacteria / genetics; CRISPR-Cas Systems / genetics; Clustered Regularly Interspaced Short Palindromic Repeats; DNA Repair; Genetic Engineering / methods; Genome, Bacterial; Homeostasis / genetics; Mutation; Phenotype
TL;DR:
This review summarizes recent progress in bacterial genome editing and identifies fundamental genetic and phenotypic outcomes of CRISPR targeting in bacteria, in the context of tool development, genome homeostasis, and DNA repair.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2015 journal article
JFS Special Issue: 75 years of advancing food science, and preparing for the next 75 CRISPR-based technologies and the future of food science
Journal of Food Science, 80(11), R2367–2372.
Source: NC State University Libraries
Added: August 6, 2018
2015 journal article
Occurrence and activity of a type II CRISPR-Cas system in Lactobacillus gasseri
MICROBIOLOGY-SGM, 161, 1752–1761.
MeSH headings : Base Sequence; CRISPR-Cas Systems / genetics; Clustered Regularly Interspaced Short Palindromic Repeats; Gene Order; Genome, Bacterial; Lactobacillus / genetics; Molecular Sequence Data; Plasmids / genetics
TL;DR:
The distribution and function of a native type II-A CRISPR-Cas system in the commensal species L. gasseri is reported, which open avenues for applications for bacteriophage protection and genome modification in L.gasseri, and contribute to the fundamental understanding of CRISpr-Cas systems in bacteria.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2015 journal article
SIGNR3-dependent immune regulation by Lactobacillus acidophilus surface layer protein A in colitis
EMBO JOURNAL, 34(7), 881–895.
author keywords: colitis; immune regulation; Lactobacillus acidophilus; SIGNR3; surface layer protein A
MeSH headings : Animals; Antigens, CD / genetics; Antigens, CD / immunology; Bacterial Proteins / genetics; Bacterial Proteins / immunology; Inflammatory Bowel Diseases / genetics; Inflammatory Bowel Diseases / immunology; Inflammatory Bowel Diseases / microbiology; Inflammatory Bowel Diseases / pathology; Intestinal Mucosa / immunology; Intestinal Mucosa / pathology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / immunology; Lectins, C-Type / genetics; Lectins, C-Type / immunology; Lipopolysaccharides / genetics; Lipopolysaccharides / immunology; Mice; Mice, Knockout; Protein Binding / genetics; Protein Binding / immunology; Teichoic Acids / genetics; Teichoic Acids / immunology
TL;DR:
This work presents critical insights into SlpA/SIGNR3‐induced responses that are integral to the potential development of novel biological therapies for autoinflammatory diseases, including IBD.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2015 article
Sortase-deficient lactobacilli: effect on immunomodulation and gut retention
Call, E. K., Goh, Y. J., Selle, K., Klaenhammer, T. R., & O'Flaherty, S. (2015, February). MICROBIOLOGY-SGM, Vol. 161, pp. 311–321.
MeSH headings : Aminoacyltransferases / genetics; Aminoacyltransferases / immunology; Aminoacyltransferases / metabolism; Animals; Bacterial Adhesion; Bacterial Proteins / genetics; Bacterial Proteins / immunology; Bacterial Proteins / metabolism; Caco-2 Cells; Cysteine Endopeptidases / genetics; Cysteine Endopeptidases / immunology; Cysteine Endopeptidases / metabolism; Dendritic Cells / immunology; Dendritic Cells / microbiology; Gastrointestinal Tract / immunology; Gastrointestinal Tract / microbiology; Humans; Immunomodulation; Lactobacillus / enzymology; Lactobacillus / genetics; Lactobacillus / growth & development; Lactobacillus / physiology; Lactobacillus acidophilus / enzymology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Lactobacillus acidophilus / physiology; Mice; Swine; Tumor Necrosis Factor-alpha / immunology
TL;DR:
It is shown for the first time that sortase-dependent proteins contribute to gut retention of probiotic microbes in the gastrointestinal tract.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Guide RNA Functional Modules Direct Cas9 Activity and Orthogonality
MOLECULAR CELL, 56(2), 333–339.
MeSH headings : Bacterial Proteins / chemistry; CRISPR-Associated Protein 9; CRISPR-Associated Proteins / chemistry; CRISPR-Cas Systems; Clustered Regularly Interspaced Short Palindromic Repeats; DNA / chemistry; DNA Cleavage; Endonucleases / chemistry; Genetic Engineering / methods; HEK293 Cells; Humans; Nucleic Acid Conformation; RNA, Guide, Kinetoplastida / chemistry; RNA, Guide, Kinetoplastida / genetics; RNA, Guide, Kinetoplastida / ultrastructure
TL;DR:
Six conserved modules within native crRNA:tracrRNA duplexes and single guide RNAs that direct Cas9 endonuclease activity are identified and characterized and it is shown the bulge and nexus are necessary for DNA cleavage and the nexus and hairpins are instrumental in defining orthogonality between systems.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2014 journal article
Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems
MBIO, 5(1).
MeSH headings : CRISPR-Cas Systems; DNA, Bacterial / genetics; DNA, Bacterial / metabolism; Escherichia coli / genetics; Genome, Bacterial; Hydrolysis; Microbial Viability
TL;DR:
It is demonstrated that genome targeting with CRISPR-Cas systems can be employed for the sequence-specific and titratable removal of individual bacterial strains and species and may open new avenues for the development of “smart” antibiotics that circumvent multidrug resistance and differentiate between pathogenic and beneficial microorganisms.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2013 review
Genomic and phenotypic evidence for probiotic influences of Lactobacillus gasseri on human health
[Review of ]. FEMS MICROBIOLOGY REVIEWS, 37(6), 915–935.
author keywords: gastrointestinal tract; acidophilus complex; bacteriocin; lactic acid bacteria; autochthonous; microbiota
MeSH headings : Animals; Bacteriocins / metabolism; Female; Gastrointestinal Tract / microbiology; Gastrointestinal Tract / physiology; Genome, Bacterial; Health; Helicobacter Infections / diet therapy; Helicobacter pylori; Homeostasis; Humans; Hypersensitivity / prevention & control; Immune System / physiology; Lactobacillus / classification; Lactobacillus / genetics; Lactobacillus / physiology; Probiotics / therapeutic use; Vagina / microbiology; Vagina / physiology; Virus Diseases / diet therapy
TL;DR:
The genomic and empirical evidence supporting use of L. gasseri in probiotic applications is substantiated by clinical trial data displaying maintenance of vaginal homeostasis, mitigation of Helicobacter pylori infection, and amelioration of diarrhea.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Identification of extracellular surface-layer associated proteins in Lactobacillus acidophilus NCFM
MICROBIOLOGY-SGM, 159, 2269–2282.
MeSH headings : Animals; Cells, Cultured; Chromatography, Liquid; Dendritic Cells / drug effects; Lactobacillus acidophilus / chemistry; Lactobacillus acidophilus / immunology; Mass Spectrometry; Membrane Glycoproteins / analysis; Membrane Glycoproteins / immunology; Mice; Tumor Necrosis Factor-alpha / metabolism
TL;DR:
P phenotypic characterization of the deletion mutant demonstrated that the SLAP LBA1029 contributes to a pro-inflammatory TNF-α response from murine DCs, and appears to impart important surface display features and immunological properties to microbes that are coated by S-layers.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
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