Works (61)

Updated: May 1st, 2024 04:52

2024 article

Maximizing Electrochemical Information: A Perspective on Background-Inclusive Fast Voltammetry

Movassaghi, C. S., Fillol, M. A., Kishida, K. T., McCarty, G., Sombers, L. A., Wassum, K. M., & Andrews, A. M. (2024, April 10). ANALYTICAL CHEMISTRY, Vol. 4.

By: C. Movassaghi*, M. Fillol, K. Kishida*, G. McCarty n, L. Sombers n, K. Wassum*, A. Andrews*

Sources: Web Of Science, NC State University Libraries
Added: April 22, 2024

2023 article

A Student Perspective on the 18th Monitoring Molecules in Neuroscience Meeting in Lyon

Forderhase, A. G., Kimble, L. C., & Sombers, L. A. (2023, June 5). ACS CHEMICAL NEUROSCIENCE, Vol. 6.

By: A. Forderhase n, L. Kimble n & L. Sombers n

author keywords: Biosensors; multiplexing; genetically encodedsensors; in vivo; neurochemistry; analyticalmethods
MeSH headings : Humans; Pandemics; COVID-19; Neurosciences
TL;DR: Some of the noteworthy techniques expediting the understanding of circuit-level neurochemical function include multiplexed detection of numerous neurochemicals, well-established sensors leveraging enzymes and other biologic components, and the development of diverse, customizable genetically encoded sensors. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: July 24, 2023

2023 article

Exploiting Microelectrode Geometry for Comprehensive Detection of Individual Exocytosis Events at Single Cells

De Alwis, A. C., Denison, J. D., Shah, R., McCarty, G. S., & Sombers, L. A. (2023, August 8). ACS SENSORS, Vol. 8.

By: A. De Alwis n, J. Denison n, R. Shah n, G. McCarty n & L. Sombers n

author keywords: voltammetry; amperometry; chromaffincell; diffusion; catecholamine; dense corevesicle; carbon fiber microelectrode
TL;DR: This work demonstrates how electrode geometry can be exploited for improved detection of exocytosis events by addressing diffusional loss─an often-overlooked source of inaccuracy in single-cell measurements. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 21, 2023

2023 journal article

Exploring Electrochemistry: A Hydrogen Peroxide Sensor Based on a Screen-Printed Carbon Electrode Modified with Prussian Blue

JOURNAL OF CHEMICAL EDUCATION, 100(12), 4853–4859.

By: J. Todorov n, G. McCarty n & L. Sombers n

author keywords: Upper-Division Undergraduate; AnalyticalChemistry; Laboratory Equipment; Hands-On Learning; Electrochemistry; Quantitative Analysis
Sources: Web Of Science, NC State University Libraries
Added: January 8, 2024

2023 article

Monitoring Molecules in Neuroscience

Sombers, L. A. (2023, October 18). ACS CHEMICAL NEUROSCIENCE, Vol. 14, pp. 3726–3727.

By: L. Sombers*

Sources: Web Of Science, NC State University Libraries
Added: November 6, 2023

2023 journal article

Simultaneous, Real-Time Detection of Glutamate and Dopamine in Rat Striatum Using Fast-Scan Cyclic Voltammetry

ACS SENSORS, 8(11), 4091–4100.

By: L. Kimble n, J. Twiddy n, J. Berger n, A. Forderhase n, G. Mccarty n, J. Meitzen n, L. Sombers n

author keywords: FSCV; biosensor; carbon-fiber microelectrode; excitatory amino acid transporter; dl-threo-beta-benzyloxyaspartic acid; dl-TBOA
TL;DR: A novel glutamate microbiosensor fabricated on a carbon-fiber microelectrode substrate and coupled with fast-scan cyclic voltammetry (FSCV) to enable the simultaneous quantification of glutamate and DA at single recording sites in the brain, which is impossible when using typical amperometric approaches. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: December 18, 2023

2023 journal article

Untapped Potential: Real-Time Measurements of Opioid Exocytosis at Single Cells

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 145(44), 24071–24080.

By: J. Denison n, A. De Alwis n, R. Shah n, G. Mccarty n & L. Sombers n

TL;DR: Comparing the results collected at the two potentials reveals unique kinetics for these two neurochemical classes at the single-vesicle level, providing unprecedented insight into key differences between catecholamine and opioid neuropeptide release from isolated chromaffin cells. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: December 11, 2023

2022 article

Neurotransmitter Readily Escapes Detection at the Opposing Microelectrode Surface in Typical Amperometric Measurements of Exocytosis at Single Cells

McCarty, G. S., Dunaway, L. E., Denison, J. D., & Sombers, L. A. (2022, June 24). ANALYTICAL CHEMISTRY, Vol. 6.

By: G. McCarty n, L. Dunaway n, J. Denison n & L. Sombers n

MeSH headings : Animals; Carbon Fiber; Catecholamines / metabolism; Cattle; Chromaffin Cells / metabolism; Exocytosis / physiology; Microelectrodes; Neurotransmitter Agents / metabolism
TL;DR: This study characterizes distinct physical and biological mechanisms by which released neurotransmitter escapes detection at the opposing microelectrode surface, while also revealing an important role for the NET in “presynaptic” modulation of neurotransmitter release. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: July 18, 2022

2022 article

Virtual Issue: Neurotoxicology

Sombers, L. A., & Patisaul, H. B. (2022, August 3). ACS CHEMICAL NEUROSCIENCE, Vol. 13, pp. 2238–2239.

By: L. Sombers* & H. Patisaul*

Sources: Web Of Science, NC State University Libraries
Added: August 15, 2022

2021 chapter

Fast-Scan for In Vivo Measurements of Neurochemical Dynamics

In M. Fakhoury (Ed.), The Brain Reward System (pp. 93–123).

By: C. Meunier n & L. Sombers n

Ed(s): M. Fakhoury

Sources: Crossref, NC State University Libraries
Added: February 6, 2021

2020 journal article

Carbon-Fiber Nanoelectrodes for Real-Time Discrimination of Vesicle Cargo in the Native Cellular Environment

ACS Nano, 14(3), 2917–2926.

By: J. Roberts n, E. Mitchell n, L. Dunaway n, G. McCarty n & L. Sombers n

author keywords: intracellular electrochemical cytometry; epinephrine; norepinephrine; dopamine; exocytosis; fast-scan cyclic voltammetry; amperometry
MeSH headings : Animals; Carbon Fiber / chemistry; Electrochemical Techniques; Electrodes; Epinephrine / chemistry; Molecular Structure; Nanotechnology; Neuroendocrine Cells / chemistry; Norepinephrine / chemistry; PC12 Cells; Rats; Single-Cell Analysis; Time Factors; Tumor Cells, Cultured
TL;DR: Carbon nanoelectrodes were developed to permit selective electrochemical sampling of nanoscale vesicles in the cell cytosol and enabled analysis of picoliter-scale biological volumes, revealing key differences between chromaffin cells at the level of the dense-core granule. (via Semantic Scholar)
UN Sustainable Development Goal Categories
16. Peace, Justice and Strong Institutions (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 18, 2021

2020 journal article

Interpreting Dynamic Interfacial Changes at Carbon Fiber Microelectrodes Using Electrochemical Impedance Spectroscopy

Langmuir, 36(15), 4214–4223.

By: C. Meunier n, J. Denison n, G. McCarty n & L. Sombers n

MeSH headings : Carbon; Carbon Fiber; Dielectric Spectroscopy; Electric Impedance; Microelectrodes
TL;DR: Electrochemical impedance spectroscopy is established as a powerful method for characterization of carbon-fiber microelectrodes, providing unprecedented insight into how real-world factors affect the electrode/solution interface. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: May 8, 2020

2020 journal article

Simultaneous voltammetric detection of glucose and lactate fluctuations in rat striatum evoked by electrical stimulation of the midbrain

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 412(24), 6611–6624.

By: A. Forderhase n, H. Styers n, C. Lee n & L. Sombers n

author keywords: Fast-scan cyclic voltammetry; Biosensor; In vivo; Carbon-fiber microelectrode; Astrocyte-to-neuron lactate shuttle; Neuroenergetics
MeSH headings : Animals; Biosensing Techniques; Corpus Striatum / chemistry; Corpus Striatum / metabolism; Electric Stimulation; Electrochemical Techniques; Glucose / analysis; Glucose / metabolism; Lactic Acid / analysis; Lactic Acid / metabolism; Male; Mesencephalon / chemistry; Mesencephalon / metabolism; Microelectrodes; Rats; Rats, Sprague-Dawley
TL;DR: These real-time measurements provide an unprecedented direct comparison of glucose and lactate dynamics in response to metabolic demand elicited by neuronal activation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 3, 2020

2019 journal article

Drift Subtraction for Fast-Scan Cyclic Voltammetry Using Double-Waveform Partial-Least-Squares Regression

Analytical Chemistry, 91(11), 7319–7327.

By: C. Meunier n, G. McCarty n & L. Sombers n

MeSH headings : Adenosine / analysis; Animals; Brain / diagnostic imaging; Dopamine / analysis; Electrochemical Techniques; Hydrogen Peroxide / analysis; Least-Squares Analysis; Male; Rats; Rats, Sprague-Dawley
TL;DR: DW-PLSR is used to improve quantification of adenosine, dopamine, and hydrogen peroxide fluctuations occurring >10 min from the initial background position, both in vitro and in vivo, and is demonstrated to be a powerful tool for evaluating and interpreting both rapid and gradual chemical changes captured in FSCV recordings over extended durations. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: June 24, 2019

2018 journal article

Carbon-Fiber Microbiosensor for Monitoring Rapid Lactate Fluctuations in Brain Tissue Using Fast-Scan Cyclic Voltammetry

Analytical Chemistry, 90(21), 12994–12999.

By: S. Smith n, S. Gosrani n, C. Lee n, G. McCarty n & L. Sombers n

MeSH headings : Animals; Biosensing Techniques / methods; Carbon Fiber / chemistry; Electrochemical Techniques / instrumentation; Electrochemical Techniques / methods; Enzymes, Immobilized / chemistry; Lactic Acid / analysis; Limit of Detection; Male; Microelectrodes; Mixed Function Oxygenases / chemistry; Neostriatum / metabolism; Rats, Sprague-Dawley; Sensitivity and Specificity
TL;DR: There is evidence that striatal lactate availability increases biphasically in response to electrical stimulation of the dopaminergic midbrain in the anesthetized rat, and this new tool for real-time detection of lactate dynamics promises to improve understanding of how lactateavailability underscores neuronal function and dysfunction. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: November 26, 2018

2018 journal article

Characterization of a Multiple-Scan-Rate Voltammetric Waveform for Real-Time Detection of Met-Enkephalin

ACS Chemical Neuroscience, 10(4), 2022–2032.

By: S. Calhoun n, C. Meunier n, C. Lee n, G. McCarty n & L. Sombers n

author keywords: carbon fiber; opioid; chromaffin cell; dopamine; cyclic voltammetry; microelectrode; FSCV
MeSH headings : Adrenal Glands / chemistry; Adrenal Glands / metabolism; Animals; Electrochemical Techniques / methods; Enkephalin, Methionine / analysis; Enkephalin, Methionine / metabolism; Male; Microinjections / methods; Organ Culture Techniques; Rats; Rats, Sprague-Dawley; Substantia Nigra / chemistry; Substantia Nigra / metabolism
TL;DR: This work evaluated the waveform parameters to increase sensitivity to methionine-enkephalin (M-ENK), an endogenous opioid neuropeptide implicated in pain, stress, and reward circuits and demonstrates how voltammetric waveforms can be customized to enhance detection of specific target analytes, broadly speaking. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: May 6, 2019

2018 journal article

Electrochemical Selectivity Achieved Using a Double Voltammetric Waveform and Partial Least Squares Regression: Differentiating Endogenous Hydrogen Peroxide Fluctuations from Shifts in pH

Analytical Chemistry, 90(3), 1767–1776.

By: C. Meunier n, E. Mitchell n, J. Roberts n, J. Toups n, G. McCarty n & L. Sombers n

MeSH headings : Animals; Brain / metabolism; Electrochemical Techniques / methods; Hydrogen Peroxide / metabolism; Hydrogen-Ion Concentration; Least-Squares Analysis; Male; Principal Component Analysis; Rats, Sprague-Dawley
TL;DR: The data demonstrate that the double waveform PLSR model is a powerful tool that can be used to disambiguate and evaluate naturally occurring H2O2 fluctuations in vivo. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 5, 2020

2018 review

Fast-Scan Cyclic Voltammetry: Chemical Sensing in the Brain and Beyond

[Review of ]. ANALYTICAL CHEMISTRY, 90(1), 490–504.

By: J. Roberts n & L. Sombers n

MeSH headings : Animals; Brain / metabolism; Catecholamines / analysis; Electrochemical Techniques / methods; Humans; Hydrogen Peroxide / analysis; Neurotransmitter Agents / analysis
TL;DR: The electroanalytical method known as fast-scan cyclic voltammetry (FSCV), how it has advanced over the years, and in what way(s) it is impacting other sciences are described. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2018 journal article

Local μ-Opioid Receptor Antagonism Blunts Evoked Phasic Dopamine Release in the Nucleus Accumbens of Rats

ACS Chemical Neuroscience, 10(4), 1935–1940.

By: A. Gómez-A*, T. Shnitko*, H. Barefoot*, E. Brightbill*, L. Sombers n, S. Nicola*, D. Robinson*

author keywords: mu-opioid receptor; dopamine; accumbens; phasic; electrochemistry; local infusion
MeSH headings : Animals; Dopamine / metabolism; Dopamine Uptake Inhibitors / administration & dosage; Dose-Response Relationship, Drug; Infusions, Intraventricular; Male; Narcotic Antagonists / administration & dosage; Nucleus Accumbens / drug effects; Nucleus Accumbens / metabolism; Peptide Fragments / administration & dosage; Rats; Rats, Long-Evans; Receptors, Opioid, mu / antagonists & inhibitors; Receptors, Opioid, mu / metabolism; Somatostatin / administration & dosage
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: May 6, 2019

2018 journal article

NMDA Receptor-Dependent Cholinergic Modulation of Mesolimbic Dopamine Cell Bodies: Neurochemical and Behavioral Studies

ACS Chemical Neuroscience, 10(3), 1497–1505.

By: M. Spanos n, X. Xie n, J. Gras-Najjar n, S. White n & L. Sombers n

author keywords: Glutamate; acetylcholine; dopamine; sensory stimuli; ventral tegmental area
MeSH headings : Animals; Cell Body / drug effects; Cell Body / metabolism; Cholinergic Agonists / administration & dosage; Dopamine / metabolism; Male; Microinjections / methods; N-Methylaspartate / administration & dosage; Photic Stimulation / methods; Psychomotor Performance / drug effects; Psychomotor Performance / physiology; Rats; Rats, Sprague-Dawley; Receptors, N-Methyl-D-Aspartate / agonists; Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors; Receptors, N-Methyl-D-Aspartate / metabolism; Ventral Tegmental Area / drug effects; Ventral Tegmental Area / metabolism
TL;DR: It is suggested that acetylcholine and glutamate synergistically act at dopamine cells in the VTA to modulate VTA-NAc shell dopaminergic output, and this underlies motivation to lever press for a motivationally neutral visual stimulus. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: April 9, 2019

2018 journal article

Quantitative Comparison of Enzyme Immobilization Strategies for Glucose Biosensing in Real-Time Using Fast-Scan Cyclic Voltammetry Coupled with Carbon-Fiber Microelectrodes

ChemPhysChem, 19(10), 1197–1204.

By: S. Smith n, L. Lugo-Morales n, C. Tang n, S. Gosrani n, C. Lee n, J. Roberts n, S. Morton n, G. McCarty n, S. Khan n, L. Sombers n

author keywords: biosensing; carbon-fiber microelectrodes; cyclic voltammetry; dopamine; glucose
MeSH headings : Animals; Biosensing Techniques; Carbon / chemistry; Electrochemical Techniques; Enzymes, Immobilized; Glucose / analysis; Glucose Oxidase / metabolism; Male; Microelectrodes; Rats; Rats, Sprague-Dawley; Time Factors
TL;DR: Comparing three approaches to enzyme immobilization on the microelectrode surface-physical adsorption, hydrogel entrapment, andEntrapment in electrospun nanofibers suggests that each of these methods can be used to create functional microbiosensors. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2018 journal article

Reducing Data Density in Fast-Scan Cyclic Voltammetry Measurements of Dopamine Dynamics

Journal of The Electrochemical Society, 165(12), G3042–G3050.

By: C. Lee n, L. Qi n, A. Amos n, K. Blanton n, G. McCarty n & L. Sombers n

UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: January 18, 2021

2017 journal article

Background Signal as an in Situ Predictor of Dopamine Oxidation Potential: Improving Interpretation of Fast-Scan Cyclic Voltammetry Data

ACS CHEMICAL NEUROSCIENCE, 8(2), 411–419.

By: C. Meunier n, J. Roberts n, G. McCarty n & L. Sombers n

author keywords: Carbon-fiber microelectrode; electrochemical impedance spectroscopy; electrochemistry; calibration; in vivo
MeSH headings : Analysis of Variance; Animals; Antipsychotic Agents / pharmacology; Biophysics; Brain / drug effects; Brain / metabolism; Dopamine / metabolism; Electric Stimulation; Electrochemical Techniques; Electrodes; Enzyme Inhibitors / pharmacology; Male; Neural Pathways / drug effects; Neural Pathways / metabolism; Oxidation-Reduction; Rats; Rats, Sprague-Dawley; Reserpine / pharmacology; alpha-Methyltyrosine / pharmacology
TL;DR: The model, validated both in adrenal slice and live brain tissue, enables information encoded in the shape of the background voltammogram to determine electrochemical parameters that are critical for accurate quantification, which improves data interpretation and provides a significant next step toward more automated methods for in vivo data analysis. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 conference paper

Real-Time Striatal Measurements of Oxidative Stress and Dopamine in the Dyskinetic Rat during Chronic L-DOPA Treatment for Parkinson's Disease

ECS Meeting Abstracts, MA2017–02 2303.

By: L. Sombers*, L. Wilson, C. Mason & C. Lee

Sources: NC State University Libraries, NC State University Libraries
Added: September 15, 2022

2017 journal article

Selective and Mechanically Robust Sensors for Electrochemical Measurements of Real-Time Hydrogen Peroxide Dynamics in Vivo

Analytical Chemistry, 90(1), 888–895.

By: L. Wilson n, S. Panda n, A. Schmidt n & L. Sombers n

MeSH headings : Animals; Carbon / chemistry; Corpus Striatum / metabolism; Electrochemical Techniques / instrumentation; Electrochemical Techniques / methods; Hydrogen Peroxide / analysis; Male; Microelectrodes; Phenylenediamines / chemistry; Rats, Sprague-Dawley
TL;DR: The data demonstrate that this technology can be used to ensure the selective detection of H2O2, enabling confident characterization of the role this molecule plays in normal physiological function as well as in the progression of PD and other neuropathies involving oxidative stress. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2017 journal article

Simultaneous Voltammetric Measurements of Glucose and Dopamine Demonstrate the Coupling of Glucose Availability with Increased Metabolic Demand in the Rat Striatum

ACS Chemical Neuroscience, 8(2), 272–280.

By: S. Smith n, C. Lee n, M. Dausch n, B. Horman n, H. Patisaul n, G. McCarty n, L. Sombers n

author keywords: Biosensor; carbon fiber microelectrode; neuroenergetics; glucose oxidase; cocaine; fast-scan cyclic voltammetry
MeSH headings : Animals; Biophysics; Cocaine / pharmacology; Corpus Striatum / cytology; Corpus Striatum / diagnostic imaging; Corpus Striatum / drug effects; Corpus Striatum / metabolism; Dopamine / metabolism; Dopamine Antagonists / pharmacology; Dopamine Uptake Inhibitors / pharmacology; Drug Combinations; Electric Stimulation; Extracellular Fluid / metabolism; Glial Fibrillary Acidic Protein / metabolism; Glucose / metabolism; Hydrogen Peroxide / metabolism; Male; Microelectrodes; Raclopride / pharmacology; Rats; Rats, Sprague-Dawley; Tyrosine 3-Monooxygenase / metabolism
TL;DR: This work evaluates several waveforms to enable the first simultaneous detection of endogenous glucose and dopamine at single recording sites, and directly demonstrates the coupling of glucose availability with increased metabolic demand. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2017 journal article

Spectroelectrochemical Characterization of the Dynamic Carbon-Fiber Surface in Response to Electrochemical Conditioning

Langmuir, 33(32), 7838–7846.

By: E. Mitchell n, L. Dunaway n, G. McCarty n & L. Sombers n

TL;DR: The data demonstrate that the carbon-fiber microstructure is dynamic, able to quickly and continuously undergo rapid structural reorganization as potential is applied, repeatedly alternating between a relatively ordered state and one that exhibits greater disorder in response to applied electrochemical potentials that span the range commonly used in voltammetric experiments. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2016 journal article

The Hydroxyl Radical is a Critical Intermediate in the Voltammetric Detection of Hydrogen Peroxide

Journal of the American Chemical Society, 138(8), 2516–2519.

By: J. Roberts n, M. Voinov n, A. Schmidt n, T. Smirnova n & L. Sombers n

Contributors: J. Roberts n, M. Voinov n, A. Schmidt n, T. Smirnova n & L. Sombers n

MeSH headings : Electrochemical Techniques / methods; Electron Spin Resonance Spectroscopy / methods; Hydrogen Peroxide / analysis; Hydrogen Peroxide / chemistry; Hydroxyl Radical / analysis; Hydroxyl Radical / chemistry; Pyrroles / chemistry; Spin Labels
TL;DR: Voltammetry was combined with electron paramagnetic resonance spectroscopy to identify and define the role of an oxygen-centered radical liberated during the oxidation of H2O2, and it was demonstrated that the hydroxyl radical is a principal contributor to the voltammetry of H1O2 as signal is attenuated when this species is trapped. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: August 6, 2018

2016 journal article

Unmasking the Effects of L-DOPA on Rapid Dopamine Signaling with an Improved Approach for Nafion Coating Carbon-Fiber Microelectrodes

ANALYTICAL CHEMISTRY, 88(16), 8129–8136.

By: L. Qi n, E. Thomas n, S. White n, S. Smith n, C. Lee n, L. Wilson n, L. Sombers n

MeSH headings : Animals; Brain / drug effects; Brain / metabolism; Carbon / chemistry; Carbon Fiber; Dopamine / analysis; Electrochemical Techniques; Electrodes, Implanted; Evoked Potentials / drug effects; Levodopa / pharmacology; Male; Microelectrodes; Parkinson Disease / metabolism; Parkinson Disease / pathology; Rats; Rats, Sprague-Dawley; Signal Transduction / drug effects
TL;DR: Overall, this work describes a reliable tool that allows a better measure of L-DOPA augmented dopamine release in vivo, measured using fast-scan cyclic voltammetry. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Enzyme-based microbiosensors for selective quantification of rapid molecular fluctuations in brain tissue

Compendium of In Vivo Monitoring in Real-Time Molecular Neuroscience, Vol 1: Fundamentals and Applications, 137–160.

By: L. Lugo-Morales n & L. Sombers n

Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2014 journal article

Multiple Scan Rate Voltammetry for Selective Quantification of Real-Time Enkephalin Dynamics

ANALYTICAL CHEMISTRY, 86(15), 7806–7812.

By: A. Schmidt n, L. Dunaway n, J. Roberts n, G. McCarty n & L. Sombers n

MeSH headings : Animals; Electrodes; Enkephalins / physiology; In Vitro Techniques; Male; Rats; Rats, Sprague-Dawley
TL;DR: This work has developed and characterized a novel voltammetric waveform for the selective quantification of small tyrosine-containing peptides, such as the ENKs, with rapid temporal (subsecond) and precise spatial (10s of micrometers) resolution, and provides a foundation for real-time measurements of endogenous ENK fluctuations in biological locations. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Peroxygenase and Oxidase Activities of Dehaloperoxidase-Hemoglobin from Amphitrite ornata

Journal of the American Chemical Society, 136(22), 7914–7925.

By: D. Barrios n, J. D’Antonio n, N. McCombs n, J. Zhao n, S. Franzen n, A. Schmidt n, L. Sombers n, R. Ghiladi n

Contributors: D. Barrios n, . J. D'Antonio, N. McCombs n, J. Zhao n, S. Franzen n, A. Schmidt n, L. Sombers n, R. Ghiladi n

MeSH headings : Animals; Catalytic Domain; Hemoglobins / chemistry; Hemoglobins / metabolism; Indoles / chemistry; Indoles / metabolism; Mixed Function Oxygenases / metabolism; Models, Molecular; Oxidoreductases / metabolism; Oxygen / chemistry; Oxygen Consumption; Oxygen Radioisotopes; Polychaeta / enzymology; Protein Conformation
TL;DR: The marine globin dehaloperoxidase‐hemoglobin (DHP) from Amphitrite ornata was found to catalyze the H2O2‐dependent oxidation of monohaloindoles, a previously unreported class of substrate for DHP, and non‐enzymatic and anaerobic controls suggest a novel oxidase activity for D HP. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2014 journal article

Reducing the Sampling Rate of Biochemical Measurements Using Fast-Scan Cyclic Voltammetry for In Vivo Applications

IEEE SENSORS JOURNAL, 14(9), 2975–2980.

By: A. Amos n, J. Roberts n, L. Qi n, L. Sombers n & G. McCarty n

author keywords: Carbon fiber microelectrodes; dopamine; electrochemistry; wireless protocol
TL;DR: Reducing the sampling rate of a proven in vivo measurement technology, fast-scan cyclic voltammetry at carbon-fiber microelectrodes, reduces the quantity of data generated by an order of magnitude compared with the existing protocol and with duty cycling is expected to decrease power consumption by a similar value in wireless systems. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

Carbon Nanotube Yarn Electrodes for Enhanced Detection of Neurotransmitter Dynamics in Live Brain Tissue

ACS NANO, 7(9), 7864–7873.

By: A. Schmidt n, X. Wang n, Y. Zhu n & L. Sombers n

author keywords: FSCV; carbon fiber; microelectrode; dopamine; voltammetry
MeSH headings : Action Potentials / physiology; Animals; Brain / physiology; Conductometry / instrumentation; Equipment Design; Equipment Failure Analysis; Male; Microelectrodes; Molecular Imaging / instrumentation; Nanofibers / chemistry; Nanotubes, Carbon / chemistry; Nanotubes, Carbon / ultrastructure; Neurotransmitter Agents / metabolism; Organ Culture Techniques; Rats; Rats, Sprague-Dawley; Reproducibility of Results; Sensitivity and Specificity; Synaptic Transmission / physiology
TL;DR: The results demonstrate that the distinct structural and electronic properties of the nanotubes result in improved selectivity, sensitivity, and spatial resolution, as well as faster apparent electron transfer kinetics when compared to the conventional carbon-fiber microelectrodes typically used in vivo. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

Enzyme-Modified Carbon-Fiber Microelectrode for the Quantification of Dynamic Fluctuations of Nonelectroactive Analytes Using Fast-Scan Cyclic Voltammetry

ANALYTICAL CHEMISTRY, 85(18), 8780–8786.

By: L. Lugo-Morales n, P. Loziuk n, A. Corder n, J. Toups n, J. Roberts n, K. McCaffrey n, L. Sombers n

MeSH headings : Animals; Biosensing Techniques / methods; Carbon / chemistry; Carbon Fiber; Electrochemical Techniques / methods; Enzyme Induction; Male; Microelectrodes; Rats; Rats, Sprague-Dawley; Time Factors
TL;DR: This work has enabled the real-time detection of dynamic glucose fluctuations in live brain tissue using background-subtracted, fast-scan cyclic voltammetry, and quantitatively and selectively monitored pharmacologically evoked glucose fluctuations with unprecedented chemical and spatial resolution. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

In Situ Electrode Calibration Strategy for Voltammetric Measurements In Vivo

ANALYTICAL CHEMISTRY, 85(23), 11568–11575.

By: J. Roberts n, J. Toups n, E. Eyualem n, G. McCarty n & L. Sombers n

MeSH headings : Animals; Calibration; Electrochemical Techniques / methods; Electrodes, Implanted; Flow Injection Analysis / methods; Male; Rats; Rats, Sprague-Dawley
TL;DR: A model that utilizes information contained in the background charging current to predict electrode sensitivity to dopamine, ascorbic acid, hydrogen peroxide, and pH shifts at any point in an electrochemical experiment is developed. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

Quantitation of Hydrogen Peroxide Fluctuations and Their Modulation of Dopamine Dynamics in the Rat Dorsal Striatum Using Fast-Scan Cyclic Voltammetry

ACS CHEMICAL NEUROSCIENCE, 4(5), 782–789.

By: M. Spanos n, J. Gras-Najjar n, J. Letchworth n, A. Sanford n, J. Toups n & L. Sombers n

author keywords: oxidative stress; reactive oxygen species; H2O2; FSCV; Parkinson's disease; basal dopamine concentrations
MeSH headings : Animals; Dopamine / metabolism; Electric Stimulation; Electrochemical Techniques; Hydrogen Peroxide / metabolism; Male; Neostriatum / drug effects; Neostriatum / metabolism; Oxidative Stress; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species / metabolism; Rotenone / pharmacology; Uncoupling Agents / pharmacology
TL;DR: Increases in endogenous H2O2 in the dorsal striatum attenuated electrically evoked DA release, and also decreased basal DA levels in this brain region will help to disambiguate the chemical mechanisms underlying the progression of neurodegenerative disease states, such as Parkinson's disease, that involve oxidative stress. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 chapter

Real-Time Chemical Measurements of Dopamine Release in the Brain

In Methods in Molecular Biology (pp. 275–294).

By: J. Roberts n, L. Lugo-Morales n, P. Loziuk n & L. Sombers n

MeSH headings : Anesthesia; Animals; Brain / metabolism; Brain / surgery; Carbon / chemistry; Carbon Fiber; Dopamine / metabolism; Electric Stimulation; Electrochemistry / instrumentation; Electrochemistry / methods; Microelectrodes; Rats; Software; Time Factors
TL;DR: This chapter describes, completely, how to make these measurements and the properties of FSCV that make it uniquely suitable for performing chemical measurements of dopaminergic neurotransmission in vivo. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 5, 2021

2012 journal article

Sources contributing to the average extracellular concentration of dopamine in the nucleus accumbens

JOURNAL OF NEUROCHEMISTRY, 121(2), 252–262.

By: C. Owesson-White*, M. Roitman*, L. Sombers n, A. Belle*, R. Keithley*, J. Peele*, R. Carelli*, R. Wightman*

author keywords: basal level; carbon-fiber; Dopamine; FSCV; transients
MeSH headings : Anesthetics, Local / administration & dosage; Anesthetics, Local / pharmacology; Animals; Dopamine / metabolism; Dopamine / physiology; Dopamine Plasma Membrane Transport Proteins / antagonists & inhibitors; Electrophysiological Phenomena; Extracellular Space / metabolism; Lidocaine / administration & dosage; Lidocaine / pharmacology; Male; Microdialysis; Nucleus Accumbens / metabolism; Patch-Clamp Techniques; Rats; Rats, Sprague-Dawley; Tetrabenazine / pharmacology; Ventral Tegmental Area; Vesicular Monoamine Transport Proteins / antagonists & inhibitors; Vesicular Monoamine Transport Proteins / metabolism; Vesicular Neurotransmitter Transport Proteins / antagonists & inhibitors; Vesicular Neurotransmitter Transport Proteins / metabolism
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Trace metal complexation by the triscatecholate siderophore protochelin: structure and stability

BIOMETALS, 25(2), 393–412.

By: J. Harrington n, J. Bargar*, A. Jarzecki*, J. Roberts n, L. Sombers n & O. Duckworth n

Contributors: J. Harrington n, J. Bargar*, A. Jarzecki*, J. Roberts n, L. Sombers n & O. Duckworth n

author keywords: Siderophores; Catechol; Iron; Manganese; Cobalt; Complexation
MeSH headings : Coordination Complexes / chemistry; Hydrogen-Ion Concentration; Oxidation-Reduction; Siderophores / chemistry; Solubility; Trace Elements / chemistry; Trace Elements / metabolism
TL;DR: The solution chemistry of protochelin and its complexes with environmentally relevant trace metals is probed to better understand its effect on metal uptake and cycling and the role of catecholate siderophores in environmental trace metal cycling and intracellular metal release is discussed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2011 journal article

Comparison of electrode materials for the detection of rapid hydrogen peroxide fluctuations using background-subtracted fast scan cyclic voltammetry

The Analyst, 136(17), 3550.

By: J. Roberts n, K. Hamilton n & L. Sombers n

MeSH headings : Biosensing Techniques / methods; Carbon / chemistry; Carbon Fiber; Electrochemical Techniques / methods; Electrodes; Fluorocarbon Polymers / chemistry; Hydrogen Peroxide / analysis; Microelectrodes; Platinum / chemistry; Sensitivity and Specificity; Time Factors
TL;DR: These results confirm that platinum electrodes, with or without Nafion(®), will not work acceptably with this approach, and confirm the value of carbon-fiber microelectrodes relative to more traditionally used platinum electrodes in the direct detection of rapid H(2)O( 2) fluctuations using FSCV. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2010 conference paper

Application of Fast-Scan Cyclic Voltammetry to Monitoring Endogenous Hydrogen Peroxide Fluctuations In Vivo

In B. Westerink, R. Clinckers, I. Smolders, S. Sarre, & Y. Michotte (Eds.), Monitoring Molecules in Neuroscience (pp. 89–91). Brussels, Belgium: Vrije Universiteit.

By: A. Sanford, J. Letchworth & L. Sombers

Ed(s): B. Westerink, R. Clinckers, I. Smolders, S. Sarre & Y. Michotte

Event: 13th International Conference on In Vivo Methods at Brussels, Belgium on September 12-16, 2010

Source: NC State University Libraries
Added: February 12, 2021

2010 journal article

Specific Oxygen-Containing Functional Groups on the Carbon Surface Underlie an Enhanced Sensitivity to Dopamine at Electrochemically Pretreated Carbon Fiber Microelectrodes

LANGMUIR, 26(11), 9116–9122.

By: J. Roberts n, B. Moody n, G. McCarty n & L. Sombers n

MeSH headings : Carbon / chemistry; Dopamine / chemistry; Electrochemistry / instrumentation; Microelectrodes; Microscopy, Atomic Force; Oxygen / chemistry; Spectrum Analysis, Raman
TL;DR: It is demonstrated that this electrochemical pretreatment can be used to create carbon microdisc electrodes with sensitivities comparable to those associated with larger, conventionally treated cylindrical carbon fiber microelectrodes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Voltammetric Detection of Hydrogen Peroxide at Carbon Fiber Microelectrodes

ANALYTICAL CHEMISTRY, 82(12), 5205–5210.

By: A. Sanford n, S. Morton n, K. Whitehouse n, H. Oara n, L. Lugo-Morales n, J. Roberts n, L. Sombers n

MeSH headings : Animals; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Brain Chemistry; Carbon / chemistry; Carbon Fiber; Electrochemistry / instrumentation; Electrochemistry / methods; Hydrogen Peroxide / analysis; Hydrogen Peroxide / metabolism; Limit of Detection; Male; Microelectrodes; Rats; Rats, Sprague-Dawley
TL;DR: The first voltammetric characterization of rapid hydrogen peroxide fluctuations at an uncoated carbon fiber microelectrode is presented, demonstrating unprecedented chemical and spatial resolution. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

Synaptic Overflow of Dopamine in the Nucleus Accumbens Arises from Neuronal Activity in the Ventral Tegmental Area

Journal of Neuroscience, 29(6), 1735–1742.

By: L. Sombers*, M. Beyene, R. Carelli & R. Mark Wightman

author keywords: in vivo voltammetry; neurotransmission; carbon-fiber microelectrode; cocaine; intracranial self-stimulation; burst firing
MeSH headings : Animals; Dopamine / physiology; Male; Neurons / physiology; Nucleus Accumbens / physiology; Rats; Rats, Sprague-Dawley; Synapses / physiology; Synaptic Transmission / physiology; Ventral Tegmental Area / physiology
TL;DR: It is shown that dopamine transients are evoked by neural activity in the ventral tegmental area (VTA), a brain region containing dopaminergic cell bodies, and how neuronal firing of dopamine neurons originating in the VTA translates into synaptic overflow in a key terminal region, the NAc shell is demonstrated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 6, 2021

2008 journal article

Dopamine Detection with Fast-Scan Cyclic Voltammetry Used with Analog Background Subtraction

Analytical Chemistry, 80(11), 4040–4048.

By: A. Hermans*, R. Keithley*, J. Kita*, L. Sombers* & R. Wightman*

MeSH headings : Analog-Digital Conversion; Anesthesia; Animals; Brain / metabolism; Brain / physiology; Carbon / chemistry; Carbon Fiber; Dopamine / analysis; Electrochemistry; Male; Microelectrodes; Principal Component Analysis; Rats; Rats, Sprague-Dawley; Time Factors; Transducers; Walking
TL;DR: It is shown that cocaine causes significant fluctuations in dopamine concentration in the brain while those for the background and pH remain unchanged from their predrug value. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2007 journal article

Dopamine release is heterogeneous within microenvironments of the rat nucleus accumbens

European Journal of Neuroscience, 26(7), 2046–2054.

By: R. Wightman*, M. Heien*, K. Wassum*, L. Sombers*, B. Aragona*, A. Khan*, J. Ariansen*, J. Cheer*, P. Phillips*, R. Carelli*

author keywords: carbon-fiber microelectrode; in vivo voltammetry; neurotransmission; temporal coding
MeSH headings : Action Potentials / physiology; Animals; Cocaine / pharmacology; Dopamine / metabolism; Dopamine Plasma Membrane Transport Proteins / metabolism; Dopamine Uptake Inhibitors / pharmacology; Electric Stimulation / methods; Electrochemistry / instrumentation; Electrochemistry / methods; Glutamate Decarboxylase / metabolism; Male; Nerve Net / metabolism; Neurons / drug effects; Neurons / physiology; Neurons / radiation effects; Nucleus Accumbens / anatomy & histology; Nucleus Accumbens / metabolism; Rats; Rats, Sprague-Dawley; Wakefulness
TL;DR: An unanticipated spatial and temporal heterogeneity of dopamine transmission within the NAc that may depend upon the firing of specific subpopulations of dopamine neurons is revealed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 6, 2021

2007 journal article

High Osmolarity andL-DOPA Augment Release via the Fusion Pore in PC12 Cells

ChemPhysChem, 8(17), 2471–2477.

By: L. Sombers*, N. Wittenberg*, M. Maxson*, K. Adams* & A. Ewing*

author keywords: amperometry; catecholamines; fusion pores; hyperosmotic solutions; membranes
MeSH headings : Animals; Biophysical Phenomena; Biophysics; Cell Membrane Structures / drug effects; Cell Membrane Structures / metabolism; Dopamine / metabolism; Exocytosis / drug effects; Levodopa / pharmacology; Models, Biological; Osmolar Concentration; PC12 Cells; Porosity / drug effects; Rats
TL;DR: The data suggest that membrane biophysics are an important component in defining the rate, duration and amount of neurotransmitter release via the fusion pore. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2007 journal article

Multicore Vesicles: Hyperosmolarity and l-DOPA Induce Homotypic Fusion of Dense Core Vesicles

Cellular and Molecular Neurobiology, 27(5), 681–685.

By: L. Sombers*, M. Maxson* & A. Ewing*

author keywords: exocytosis; secretory granules; electron microscopy; PC12; cationized ferritin; quantal size; amperometry; catecholamines
MeSH headings : Animals; Dopamine Agents / pharmacology; Exocytosis / drug effects; Exocytosis / physiology; Levodopa / pharmacology; Membrane Fusion / drug effects; Microscopy, Electron, Transmission; Neurons / drug effects; Neurons / ultrastructure; Osmolar Concentration; PC12 Cells; Rats; Saline Solution, Hypertonic / pharmacology; Secretory Vesicles / drug effects; Secretory Vesicles / ultrastructure
TL;DR: The results suggest that the multicored vesicles are formed from homotypic fusion of LCDVs prior to exocytosis, and amperometric measurements show a bimodal distribution of quantal sizes in treated cells. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2007 journal article

Phasic Dopamine Release Evoked by Abused Substances Requires Cannabinoid Receptor Activation

Journal of Neuroscience, 27(4), 791–795.

By: J. Cheer*, K. Wassum*, L. Sombers*, M. Heien*, J. Ariansen*, B. Aragona*, P. Phillips*, R. Wightman*

author keywords: rimonabant; dopamine; cocaine; nicotine; ethanol; cyclic voltammetry
MeSH headings : Animals; Cannabinoid Receptor Modulators / agonists; Cannabinoid Receptor Modulators / antagonists & inhibitors; Cannabinoid Receptor Modulators / metabolism; Dopamine / metabolism; Illicit Drugs / pharmacology; Nucleus Accumbens / drug effects; Nucleus Accumbens / metabolism; Piperidines / pharmacology; Pyrazoles / pharmacology; Rats; Rats, Sprague-Dawley; Receptor, Cannabinoid, CB1 / agonists; Receptor, Cannabinoid, CB1 / antagonists & inhibitors; Receptor, Cannabinoid, CB1 / metabolism; Rimonabant; Time Factors
TL;DR: Differences in the temporal profile of dopamine concentration transients caused by acute doses of nicotine, ethanol, and cocaine in the nucleus accumbens shell of freely moving rats are demonstrated, suggesting that an increase in endocannabinoid tone facilitates the effects of commonly abused drugs on subsecond dopamine release. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 6, 2021

2006 conference paper

Burst Firing of Midbrain Dopamine Neurons Contributes to Transient Dopamine Fluctuations Time-Locked to Behavioral Responses in the Nucleus Accumbens of Freely-Moving Rats

In G. Di Chiara, E. Carboni, V. Valentini, E. Acquas, V. Bassareo, & C. Cadoni (Eds.), Monitoring molecules in neuroscience: proceeding of the 11.th International conference on in Vivo Methods 2006 : University of Cagliari, Italy (pp. 560–562). Italy: Grafiche Sainas.

By: L. Sombers, M. Beyene, J. Ariansen, C. Owesson White, J. Cheer & R. Wightman

Ed(s): G. Di Chiara, E. Carboni, V. Valentini, E. Acquas, V. Bassareo & C. Cadoni

Event: 11th International Conference on In Vivo Methods at Cagliari, Sardinia, Italy on May 19-22, 2006

Source: NC State University Libraries
Added: February 12, 2021

2005 journal article

Correlation between Vesicle Quantal Size and Fusion Pore Release in Chromaffin Cell Exocytosis

Biophysical Journal, 88(6), 4411–4420.

By: C. Amatore*, S. Arbault*, I. Bonifas*, Y. Bouret*, M. Erard*, A. Ewing*, L. Sombers*

MeSH headings : Animals; Biophysical Phenomena; Biophysics; Catecholamines / metabolism; Cattle; Chromaffin Cells / metabolism; Chromaffin Cells / physiology; Electrophysiology; Exocytosis / physiology; In Vitro Techniques; Kinetics; Membrane Fusion / physiology; Models, Biological; Secretory Vesicles / physiology
TL;DR: It is hypothesized that in chromaffin cells, the expanding core is a controlling factor in opening the fusion pore, that prefusion of two smaller vesicles leads to excess membrane, and that this slows pore expansion leading to an increased observation of events with a foot. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 5, 2021

2005 journal article

Loaded dopamine is preferentially stored in the halo portion of PC12 cell dense core vesicles

Journal of Neurochemistry, 93(5), 1122–1131.

By: L. Sombers*, M. Maxson* & A. Ewing*

author keywords: amperometry; dense core vesicles; exocytosis; PC12 cells; vesicular volume
MeSH headings : Animals; Catecholamines / metabolism; Dopamine / pharmacokinetics; Dopamine Agents / pharmacology; Electrochemistry; Extracellular Fluid / metabolism; Levodopa / pharmacology; Models, Theoretical; Osmolar Concentration; Osmosis; PC12 Cells / metabolism; Rats; Secretory Vesicles / metabolism; Tissue Distribution
TL;DR: Evidence is presented indicating that upon treatment with L‐DOPA the majority of the dopamine loaded into these vesicles is preferentially compartmentalized into the halo portion of the vesicle, suggesting that newly loaded dopamine is located in the h Halo of thevesicle. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 6, 2021

2004 journal article

The Effects of Vesicular Volume on Secretion through the Fusion Pore in Exocytotic Release from PC12 Cells

Journal of Neuroscience, 24(2), 303–309.

By: L. Sombers*

author keywords: fusion pore; exocytosis; amperometry; transmission electron microscopy; PC12 cells; vesicle volume; foot
MeSH headings : Action Potentials; Animals; Cell Membrane / ultrastructure; Cell Size; Exocytosis; Membrane Fusion; Neurons / chemistry; Neurons / physiology; Neurons / ultrastructure; Neurotransmitter Agents / analysis; Neurotransmitter Agents / metabolism; PC12 Cells; Rats; Secretory Vesicles / chemistry; Secretory Vesicles / metabolism; Secretory Vesicles / ultrastructure; Synapses / metabolism
TL;DR: First evidence indicating that vesicular volume before secretion is strongly correlated with the characteristics of amperometric foot events is presented, providing new insight into how vesicle volume can modulate the activity of the exocytotic fusion pore is provided. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 5, 2021

2004 chapter

The Modulation of Vesicular Volume and its Effect on Neurotransmitter Secretion through the Fusion Pore in PC12 Cells

In R. Borges & L. Gandia (Eds.), Cell Biology of the Chromaffin Cell (pp. 43–46). Spain: Instituto Teófilo Hernando.

By: L. Sombers, H. Hanchar, T. Colliver, N. Wittenberg, A. Cans, S. Arbault, C. Amatore, A. Ewing

Ed(s): R. Borges & L. Gandia

Source: NC State University Libraries
Added: February 12, 2021

2003 conference paper

Amperometric Detection of Exocytosis at Cells and Artificial Cells

In J. Kehr, K. Fuxe, U. Ungerstedt, & T. Svensson (Eds.), Monitoring molecules in neuroscience : proceedings of the 10th International Conference on In Vivo Methods : Karolinska Institutet, Stockholm, Sweden June 24-27, 2003 (pp. 171–173). Stockholm, Sweden: Karolinska University Press.

By: A. Ewing, A. Cans, L. Sombers, N. Wittenberg, D. Eves, R. Karlsson, M. Karlsson, A. Karlsson, O. Orwar

Ed(s): J. Kehr, K. Fuxe, U. Ungerstedt & T. Svensson

Event: 10th International Conference on In Vivo Methods at Stockholm, Sweden on June 24-27, 2003

Source: NC State University Libraries
Added: February 12, 2021

2003 journal article

Artificial cells: Unique insights into exocytosis using liposomes and lipid nanotubes

Proceedings of the National Academy of Sciences, 100(2), 400–404.

By: A. Cans*, N. Wittenberg*, R. Karlsson*, L. Sombers*, M. Karlsson*, O. Orwar*, A. Ewing*

MeSH headings : Animals; Biological Transport; Exocytosis / physiology; Lipid Metabolism; Liposomes / metabolism; Membrane Fusion; Microscopy, Fluorescence; PC12 Cells; Rats
TL;DR: The use of liposome–lipid nanotube networks are described to create an artificial cell model that undergoes the later stages of exocytosis and shows that membrane mechanics, without protein intervention, can drive expansion of the fusion pore to the final stage of excycletosis. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 5, 2021

2003 conference paper

Vesicular Volume, the Fusion Pore and Potential ‘Presynaptic’ Mechanisms Modulating Neurotransmitter Release

In J. Kehr, K. Fuxe, U. Ungerstedt, & T. Svensson (Eds.), Monitoring molecules in neuroscience : proceedings of the 10th International Conference on In Vivo Methods : Karolinska Institutet, Stockholm, Sweden June 24-27, 2003 (pp. 189–191). Stockholm, Sweden: Karolinska University Press.

By: L. Sombers, M. Maxson & A. Ewing

Ed(s): J. Kehr, K. Fuxe, U. Ungerstedt & T. Svensson

Event: 10th International Conference on In Vivo Methods at Stockholm, Sweden on June 24-27, 2003

Source: NC State University Libraries
Added: February 12, 2021

2002 journal article

Differentiated PC12 Cells

Annals of the New York Academy of Sciences, 971(1), 86–88.

By: L. Sombers*, T. Colliver* & A. Ewing*

author keywords: PC 12; differentiation; varicosity; exocytosis; VMAT; catecholamine
MeSH headings : Animals; Catecholamines / metabolism; Cell Communication; Cell Differentiation; Electrophysiology / methods; Exocytosis; Membrane Glycoproteins / metabolism; Membrane Glycoproteins / physiology; Membrane Transport Proteins; Neurons / cytology; Neurons / physiology; Neuropeptides; PC12 Cells; Rats; Time Factors; Vesicular Biogenic Amine Transport Proteins
TL;DR: ! " # $% $ " & $ $ " ' " $ " ( $) ! ! & * $ ' $ " + $ % , $" $ $ "" $ *% $ & $ "" , $ " ! " &, $ " $ , % && $ " , " & , " *" & $ % * $ ! "& " $ $ . (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 5, 2021

2002 chapter

Electrochemical Monitoring of Exocytosis from Individual PC12 Cells in Culture

In A. Brajter-Toth & J. Q. Chambers (Eds.), Electroanalytical Methods for Biological Materials (pp. 279–327).

By: L. Sombers* & A. Ewing

Ed(s): A. Brajter-Toth & J. Chambers

UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 6, 2021

2001 chapter

Electrochemical or Separations-Based Sensing of Neurotransmitter Dynamics and Storage at Cells and Varicosities in Culture

In W. T. O'Connor, J. P. Lowry, J. J. O'Connor, & R. D. O'Neill (Eds.), Monitoring molecules in neuroscience : proceedings of the 9th International Conference on In Vivo Methods, June 16-19, 2001, University College Dublin, Ireland (pp. 57–58). Dublin: University College Dublin.

By: A. Ewing, L. Sombers, L. Woods, T. Colliver, M. Achalabun, K. Moran, J. Lapos, T. Paxon, A. Cans

Ed(s): W. O'Connor, J. Lowry, J. O'Connor & R. O'Neill

Event: 9th International Conference on In Vivo Methods at Dublin, Ireland on June 16-19, 2001

Source: NC State University Libraries
Added: February 12, 2021

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