Leonard Collins
Chemistry, Mass Spectrometry, Proteomics
Works (49)
Updated: December 9th, 2024 08:35
2024 journal article
Myeloid cell MHC I expression drives CD8<SUP>+</SUP> T cell activation in nonalcoholic steatohepatitis
FRONTIERS IN IMMUNOLOGY, 14.
Contributors: T. Williams n , V. Adams n, n , J. Holmes n, P. Hess n , H. Atkins n, G. Scheidemantle n, X. Liu n
Ed(s): T. Williams n , n & A. Kennedy n
author keywords: liver; NASH; fibrosis; H2Kb; CD8(+) T cells; immunopeptidome
TL;DR:
Activated hepatic CD8+ T cells are dependent on myeloid cell MHC class I expression in diet induced NASH to promote inflammation and fibrosis and a role of NADPH oxidase in the production of Ncf2 peptide generation is suggested.
(via Semantic Scholar)
open access via www.frontiersin.org (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 28, 2024
2024 journal article
The biochemical effects of carotenoids in orange carrots on the colonic proteome in a mouse model of diet-induced obesity
FRONTIERS IN NUTRITION, 11.
author keywords: carotenoids; quantitative proteomics; colon; intestinal barrier; diet-induced obesity
Sources: Web Of Science, NC State University Libraries
Added: December 2, 2024
2023 article
Discovery Proteomics and Absolute Protein Quantification Can Be Performed Simultaneously on an Orbitrap-Based Mass Spectrometer
Williams, T. I., Kowalchyk, C., Collins, L. B., & Reading, B. J. (2023, March 22). ACS OMEGA, Vol. 3.
Contributors: T. Williams n , C. Kowalchyk*, n & B. Reading n
TL;DR:
A comparative study on data-dependent acquisition (DDA) and PRM-based workflows to quantify egg yolk protein precursors or vitellogenins (VTGs) Aa, Ab, and C in striped bass (Morone saxatilis) concludes that DDA is able to deliver both discovery data and absolute quantification data in the same experiment.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: March 23, 2023
2023 article
Discovery of a Hybrid System for Photocatalytic CO<sub>2</sub> Reduction via Attachment of a Molecular Cobalt-Quaterpyridine Complex to a Crystalline Carbon Nitride
McGuigan, S., Tereniak, S. J., Donley, C. L., Smith, A., Jeon, S., Zhao, F., … Maggard, P. A. (2023, October 12). ACS APPLIED ENERGY MATERIALS, Vol. 10.
author keywords: hybrid materials; photocatalysis; carbon nitride; CO2 reduction
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 20, 2023
2023 journal article
Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach
MICROBIAL CELL FACTORIES, 22(1).
Contributors: D. Durmusoglu n, I. Al’Abri n, Z. Li n, T. Islam Williams n, n , J. Martínez *, N. Crook n
MeSH headings : Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Saccharomyces boulardii / genetics; Saccharomyces boulardii / metabolism; Saccharomyces / genetics; Saccharomyces / metabolism; Probiotics / metabolism; Endopeptidases / metabolism
TL;DR:
A quadruple protease-deficient Sb strain is constructed that produces 5045 mg/L secretory NPA, an improvement of tenfold over wild-type Sb and highlights the ability of proteomics to highlight under-explored mediators of this process.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: June 19, 2023
2023 journal article
Pseudo-affinity capture of K. phaffii host cell proteins in flow-through mode: Purification of protein therapeutics and proteomic study
Separation and Purification Technology, 326, 124777.
Contributors: S. Sripada n, D. Elhanafi*, n , T. Williams n , M. Linova *, J. Woodley *, C. Boi n , S. Menegatti n
author keywords: Komagataella phaffii; Chromatography; Protein purification; Monoclonal antibodies; Antibody fragment; Proteomics
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: October 23, 2023
2022 article
A bottom-up proteomics workflow for a system containing multiple organisms
Bai, H., Collins, L. B. B., Andre, M. R., Breitschwerdt, E. B. B., & Williams, T. I. (2023, January 3). RAPID COMMUNICATIONS IN MASS SPECTROMETRY, Vol. 1.
Contributors: H. Bai n, n , M. André n, E. Breitschwerdt n & T. Williams n
TL;DR:
A bottom-up proteomics workflow was developed to study a system containing multiple organisms to promote a thorough understanding of how each interacts with the others and can be readily applied to other label-free proteomics work involving multiple proteomes from taxonomically distinct organisms.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: November 25, 2022
2022 article
Improving Therapeutic Protein Secretion in the Probiotic YeastSaccharomyces boulardiiusing a Multifactorial Engineering Approach
Durmusoglu, D., Al’Abri, I., Williams, T. I., Collins, L. B., Martínez, J. L., & Crook, N. (2022, December 31). BioRxiv.
Contributors: D. Durmusoglu n, I. Al'Abri n, T. Williams n , n , J. Martínez * & N. Crook n
Source: ORCID
Added: January 1, 2023
2022 journal article
Salinity-induced ionoregulatory changes in the gill proteome of the mayfly, Neocloeon triangulifer
ENVIRONMENTAL POLLUTION, 316.
Contributors: S. Orr n, n , D. Jima n & D. Buchwalter n
author keywords: Proteomics; Salinity; Mayfly; Gill biology; Ion transport; Osmoregulation
MeSH headings : Animals; Ephemeroptera; Gills / metabolism; Salinity; Proteome / metabolism; Sodium Chloride / metabolism; Proteomics; Calcium Chloride; Water Pollutants, Chemical / metabolism; Aquatic Organisms / metabolism; Sodium-Potassium-Exchanging ATPase / metabolism; Ions / metabolism; Water / metabolism
TL;DR:
The breadth of physiological functions in gills is demonstrated by exploring non-transport related pathways found in the dataset, including ATP synthesis, calcium signaling, and oxidative stress response, and the challenges of working with non-model species without fully sequenced and annotated genomes are discussed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 30, 2023
2022 journal article
Self-sacrificial tyrosine cleavage by an Fe:Mn oxygenase for the biosynthesis of para-aminobenzoate in Chlamydia trachomatis
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 119(39).
Contributors: T. Williams n
author keywords: folate biosynthesis; radical transfer; spectroscopy; heterobinuclear cluster
MeSH headings : Bacterial Proteins / metabolism; Chlamydia trachomatis / enzymology; Folic Acid; Iron / metabolism; Manganese / metabolism; Oxygen / metabolism; Oxygenases / metabolism; Tyrosine / metabolism; para-Aminobenzoates / metabolism
TL;DR:
High in vitro activity relies on the stoichiometric addition of both Fe and Mn, and CADD is shown to activate O2 to self-sacrificially produce pABA from Tyr27.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: September 21, 2022
2022 article
Towards a standardized multi-tissue decellularization protocol for the derivation of extracellular matrix materials
Biehl, A., Martins, A. M. G. M., Davis, Z. G. G., Sze, D., Collins, L., Mora-Navarro, C., … Freytes, D. O. O. (2022, December 12). BIOMATERIALS SCIENCE, Vol. 12.
MeSH headings : Tissue Engineering / methods; Proteomics; Extracellular Matrix / chemistry; Hydrogels / chemistry; Biocompatible Materials / analysis; Tissue Scaffolds
TL;DR:
The present study is the first proof-of-concept towards the development of a standardized protocol that can be used to derive multiple ECM biomaterials via a previously established automated system and showed gelation and rheological properties consistent with previous ECM hydrogels.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 3, 2023
2022 journal article
Using Proteomic Approaches to Unravel the Response of Ctenocephalides felis felis to Blood Feeding and Infection With Bartonella henselae
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 12.
Contributors: M. André n, P. Neupane n, M. Lappin *, B. Herrin*, V. Smith*, T. Williams n , n , H. Bai n
author keywords: bartonellosis; cat flea; cat scratch disease; flea-pathogen interface; proteome
MeSH headings : Animals; Bartonella henselae / genetics; Cat Diseases; Cats; Ctenocephalides; Felis; Proteomics; Siphonaptera
TL;DR:
Flea-differentially abundant proteins (FDAP) are investigated in unfed fleas, fleas fed on uninfected cats, and flea fed on B. henselae-infected cats to help understand the complex interactions between cat fleas and B. Henselae over an infection time course, thus helping understand the dual effect of vaccine targeting both flea fitness and pathogen competence.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 29, 2022
2021 journal article
PFOS dominates PFAS composition in ambient fine particulate matter (PM2.5) collected across North Carolina nearly 20 years after the end of its US production
ENVIRONMENTAL SCIENCE-PROCESSES & IMPACTS, 23(4), 580–587.
MeSH headings : Alkanesulfonic Acids / analysis; Canada; Europe; Fluorocarbons / analysis; North Carolina; Particulate Matter; Tandem Mass Spectrometry
TL;DR:
This is the first US study to provide insights into ambient PFAS concentrations in PM2.5, and the presence of PFOS in these samples raises questions about their sources, since PFOS production was phased out in the US ∼20 years ago.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 10, 2021
2018 journal article
Development of a hydrophilic interaction liquid chromatography (HILIC) method for the chemical characterization of water-soluble isoprene epoxydiol (IEPOX)-derived secondary organic aerosol
Environmental Science: Processes & Impacts, 20(11), 1524–1536.
MeSH headings : Aerosols / chemistry; Butadienes / chemistry; Catalysis; Chromatography, Liquid / methods; Epoxy Compounds / chemistry; Hemiterpenes / chemistry; Hydrophobic and Hydrophilic Interactions; Isomerism; Particulate Matter / chemistry; Sulfates / chemistry
TL;DR:
A hydrophilic interaction liquid chromatography (HILIC)/ESI-HR-quadrupole time-of-flight mass spectrometry (QTOFMS) protocol that can chromatographically resolve and accurately measure the major IEPOX-derived SOA constituents in both laboratory-generated SOA and atmospheric PM2.5 is developed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2017 journal article
A purified MAA-based ELISA is a useful tool for determining anti-MAA antibody titer with high sensitivity
PLOS ONE, 12(2), e0172172.
Ed(s): C. Hagemeyer
MeSH headings : Acetaldehyde / immunology; Animals; Autoantibodies / blood; Autoantibodies / immunology; Enzyme-Linked Immunosorbent Assay / methods; Female; Magnetic Resonance Spectroscopy; Malondialdehyde / immunology; Mice; Mice, Inbred C57BL; Sensitivity and Specificity
TL;DR:
It is demonstrated that pure MAA-modified residues can be chemically conjugated to large proteins without by-product contamination and established a purified M AA-ELISA, with which a marked increase in anti-MAA antibody titer was determined at a very early stage of atherosclerosis in 3-month ApoE-/- mice fed with a normal diet.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2017 journal article
Coordinated regulation of intracellular pH by two glucose-sensing pathways in yeast
Journal of Biological Chemistry, 293(7), 2318–2329.
MeSH headings : Cytoplasm / chemistry; Cytoplasm / metabolism; Gene Expression Regulation, Fungal; Glucose / metabolism; Hydrogen-Ion Concentration; Monosaccharide Transport Proteins / genetics; Monosaccharide Transport Proteins / metabolism; Proton-Translocating ATPases / genetics; Proton-Translocating ATPases / metabolism; Saccharomyces cerevisiae / chemistry; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Saccharomyces cerevisiae Proteins / genetics; Saccharomyces cerevisiae Proteins / metabolism
TL;DR:
The findings support the emerging view that cellular systems have evolved the use of pH signals as a means of adapting to environmental stresses such as those caused by hypoxia, ischemia, and diabetes.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2017 journal article
DNA Product Formation in Female Sprague–Dawley Rats Following Polyhalogenated Aromatic Hydrocarbon (PHAH) Exposure
Chemical Research in Toxicology, 30(3), 794–803.
MeSH headings : Animals; DNA / drug effects; Female; Polycyclic Aromatic Hydrocarbons / toxicity; Rats; Rats, Sprague-Dawley
TL;DR:
Exposure to TCDD significantly induced 1,N6-εdAdo at 31 and 53 weeks, while no increase of 8-oxo-dGuo was observed and the simple TEF methodology cannot be applied to evaluate the diverse patterns of toxic effects induced by DLCs.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2017 journal article
Evidence that endogenous formaldehyde produces immunogenic and atherogenic adduct epitopes
Scientific Reports, 7(1).
MeSH headings : Animals; Apolipoproteins E / deficiency; Atherosclerosis / immunology; Atherosclerosis / metabolism; Chromatography, Liquid; Epitopes / immunology; Formaldehyde / chemistry; Formaldehyde / metabolism; Humans; Magnetic Resonance Spectroscopy; Mass Spectrometry; Mice; Mice, Knockout; Molecular Structure; Peroxidase / metabolism; Plaque, Atherosclerotic / immunology; Plaque, Atherosclerotic / metabolism
TL;DR:
The results strongly suggest that the 1,4-dihydropyridine-type of lysine adducts observed in atherosclerosis lesions are likely produced by endogenous formaldehyde and malondialdehyde with lysines, which may play important roles in human inflammatory and degenerative diseases.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2017 journal article
Nontarget Analysis Reveals a Bacterial Metabolite of Pyrene Implicated in the Genotoxicity of Contaminated Soil after Bioremediation
Environmental Science & Technology, 51(12), 7091–7100.
MeSH headings : Biodegradation, Environmental; Polycyclic Aromatic Hydrocarbons; Pyrenes; Soil; Soil Microbiology; Soil Pollutants
TL;DR:
This work pursued a nontarget analytical approach combining effect-directed analysis (EDA) and metabolite profiling to compare extracts of PAH-contaminated soil from a former manufactured-gas plant site before and after treatment in a laboratory-scale aerobic bioreactor, and found a compound purified from soil extracts was determined to be genotoxic.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2017 journal article
Time-matched analysis of DNA adduct formation and early gene expression as predictive tool for renal carcinogenesis in methylazoxymethanol acetate treated Eker rats
Archives of Toxicology, 91(10), 3427–3438.
author keywords: Methylazoxymethanol acetate; DNA adducts; Kidney cancer; Eker rat; Biomarkers of effect; Biomarkers of exposure
MeSH headings : Animals; Cell Proliferation / drug effects; Cell Transformation, Neoplastic / drug effects; Cell Transformation, Neoplastic / pathology; DNA Adducts / analysis; DNA Damage / drug effects; Dose-Response Relationship, Drug; Female; Gene Expression Regulation, Neoplastic; Guanine / analogs & derivatives; Guanine / metabolism; Kidney / drug effects; Kidney / metabolism; Kidney / pathology; Male; Methylazoxymethanol Acetate / administration & dosage; Methylazoxymethanol Acetate / toxicity; Rats, Mutant Strains; Time Factors
TL;DR:
It is hypothesize that the combined analysis of pro-mutagenic DNA adducts along with time-matched gene expression changes could serve as a superior prediction tool for genotoxic carcinogenesis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2016 journal article
Oxidative stress at low levels can induce clustered DNA lesions leading to NHEJ mediated mutations
Oncotarget, 7(18), 25377–25390.
author keywords: oxidative stress; NHEJ; mutations; clustered DNA lesions; double strand breaks
MeSH headings : Animals; Cell Line; Chickens; DNA Damage / physiology; DNA End-Joining Repair / physiology; Hydrogen Peroxide / toxicity; Mutagenesis / physiology; Oxidants / toxicity; Oxidative Stress / physiology
TL;DR:
The results indicate for the first time that H2O2, even at low levels, can also cause OCDLs leading to DSBs specifically in G1 cells, and a reverse genetic approach revealed a significant contribution of the non-homologous end joining (NHEJ) pathway in H 2O2-induced DNA repair & mutagenesis.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2016 journal article
Polychlorinated Biphenyls Induce Oxidative DNA Adducts in Female Sprague–Dawley Rats
Chemical Research in Toxicology, 29(8), 1335–1344.
MeSH headings : Animals; Chromatography, Liquid; DNA Adducts / metabolism; Female; Oxidative Stress / drug effects; Polychlorinated Biphenyls / toxicity; Rats; Rats, Sprague-Dawley; Tandem Mass Spectrometry
TL;DR:
Increased hepatic oxidative DNA adducts following exposure to PCB 126, PCB 153, or the binary mixture shows that an increase in DNA damage may play an important role in hepatic toxicity and carcinogenesis in female Sprague-Dawley rats.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2015 journal article
Implication of a Chromosome 15q15.2 Locus in Regulating UBR1 and Predisposing Smokers to MGMT Methylation in Lung
Cancer Research, 75(15), 3108–3117.
MeSH headings : Base Sequence; Chromosomes, Human, Pair 15 / genetics; DNA Methylation; DNA Modification Methylases / genetics; DNA Modification Methylases / metabolism; DNA Repair Enzymes / genetics; DNA Repair Enzymes / metabolism; Epithelial Cells / drug effects; Female; Gene Expression Regulation; Genetic Predisposition to Disease; Genome-Wide Association Study; Guanine / analogs & derivatives; Guanine / metabolism; Humans; Longitudinal Studies; Lung Neoplasms / genetics; Male; Methylation; Methylnitrosourea / pharmacology; Molecular Sequence Data; Polymorphism, Single Nucleotide; Smoking / genetics; Tumor Suppressor Proteins / genetics; Tumor Suppressor Proteins / metabolism; Ubiquitin-Protein Ligases / genetics
TL;DR:
Results substantiate reduced gene transcription as a major mechanism for predisposition to MGMT methylation in the lungs of smokers, and support the importance of UBR1 in regulating MGMT homeostasis and DNA repair of alkylated DNA adducts in cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2014 journal article
A Gnotobiotic Mouse Model Demonstrates That Dietary Fiber Protects against Colorectal Tumorigenesis in a Microbiota- and Butyrate-Dependent Manner
Cancer Discovery, 4(12), 1387–1397.
MeSH headings : Animals; Butyrates / metabolism; Carcinogens / administration & dosage; Cell Transformation, Neoplastic; Colorectal Neoplasms / etiology; Colorectal Neoplasms / pathology; Dietary Fiber; Disease Models, Animal; Germ-Free Life; Humans; Intestinal Mucosa / pathology; Mice; Microbiota; Neoplasm Grading
TL;DR:
The results, which link diet and microbiota to a tumor-suppressive metabolite, provide insight into conflicting epidemiologic findings and suggest that probiotic/prebiotic strategies can modulate an endogenous HDAC inhibitor for anticancer chemoprevention without the adverse effects associated with synthetic HDAC inhibitors used in chemotherapy.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2014 journal article
Comparative Analysis of the Relationship Between Trichloroethylene Metabolism and Tissue-Specific Toxicity Among Inbred Mouse Strains: Kidney Effects
Journal of Toxicology and Environmental Health, Part A, 78(1), 32–49.
MeSH headings : Animals; Carcinogens / pharmacokinetics; Carcinogens / toxicity; Cell Proliferation / drug effects; Cysteine / analogs & derivatives; Cysteine / metabolism; Cytochrome P-450 Enzyme System / genetics; Cytochrome P-450 Enzyme System / metabolism; Dichloroacetic Acid / metabolism; Ethylene Chlorohydrin / analogs & derivatives; Ethylene Chlorohydrin / metabolism; Glutathione / analogs & derivatives; Glutathione / metabolism; Hepatitis A Virus Cellular Receptor 1; Kidney / cytology; Kidney / drug effects; Kidney / metabolism; Male; Membrane Proteins / genetics; Membrane Proteins / metabolism; Mice; Mice, Inbred C57BL; Mice, Inbred Strains; Oxidation-Reduction / drug effects; PPAR alpha / genetics; PPAR alpha / metabolism; Trichloroacetic Acid / metabolism; Trichloroethylene / pharmacokinetics; Trichloroethylene / toxicity
TL;DR:
Overall, data demonstrated that renal TCE metabolite levels are associated with kidney-specific toxicity and that these effects are strain dependent.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2014 journal article
Comparative Analysis of the Relationship Between Trichloroethylene Metabolism and Tissue-Specific Toxicity Among Inbred Mouse Strains: Liver Effects
Journal of Toxicology and Environmental Health, Part A, 78(1), 15–31.
MeSH headings : Administration, Oral; Animals; Carcinogens / pharmacokinetics; Carcinogens / toxicity; Cell Proliferation; Cysteine / analogs & derivatives; Cysteine / blood; Cytochrome P-450 Enzyme System / genetics; Cytochrome P-450 Enzyme System / metabolism; Dichloroacetic Acid / blood; Dose-Response Relationship, Drug; Ethylene Chlorohydrin / analogs & derivatives; Ethylene Chlorohydrin / metabolism; Gene Expression; Glutathione / analogs & derivatives; Glutathione / blood; Hepatocytes / drug effects; Hepatocytes / metabolism; Liver / cytology; Liver / drug effects; Liver / metabolism; Male; Mice; Mice, Inbred C57BL; Mice, Inbred Strains; Oxidation-Reduction / drug effects; Oxidative Stress / drug effects; Real-Time Polymerase Chain Reaction; Solvents / pharmacokinetics; Solvents / toxicity; Trichloroacetic Acid / blood; Trichloroethylene / pharmacokinetics; Trichloroethylene / toxicity
TL;DR:
A hypothesis was tested that amounts of oxidative metabolites of TCE in mouse liver are associated with hepatic-specific toxicity, and the magnitude of induction of peroxisome proliferator-marker genes declined, but hepatocellular proliferation increased.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2014 journal article
Guanine- 5-carboxylcytosine base pairs mimic mismatches during DNA replication
Scientific Reports, 4(1).
MeSH headings : 5-Methylcytosine / metabolism; Base Pairing / genetics; Cell Proliferation / genetics; Cytosine / analogs & derivatives; Cytosine / metabolism; DNA / genetics; DNA Mismatch Repair / genetics; DNA Replication / genetics; Epigenesis, Genetic / genetics; Guanine / metabolism; Humans; Thymine DNA Glycosylase / metabolism
TL;DR:
It is shown that although 5caC pairs with guanine during DNA replication in vitro, G·5caC Pair stimulated DNA polymerase exonuclease activity and were recognized by the mismatch repair (MMR) proteins, indicating MMR is a novel reader for 5CAC.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2014 journal article
Molecular Dosimetry of Endogenous and Exogenous O6-Methyl-dG and N7-Methyl-G Adducts Following Low Dose [D3]-Methylnitrosourea Exposures in Cultured Human Cells
Chemical Research in Toxicology, 27(4), 480–482.
MeSH headings : Cells, Cultured; Deoxyguanosine / analogs & derivatives; Deoxyguanosine / metabolism; Dose-Response Relationship, Drug; Guanine / analogs & derivatives; Guanine / metabolism; Humans; Methylnitrosourea / administration & dosage; Methylnitrosourea / pharmacology
TL;DR:
The data show that O6-methyl-dG adducts ≥1.8/108 dG correlated with published studies that demonstrated significant increases of mutations under these conditions, but the combined results do not support linear extrapolations to zero when data are available for science-based regulations.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2014 journal article
The endogenous exposome
DNA Repair, 19, 3–13.
author keywords: Endogenous exposome; DNA damage; Stable isotopes; Mutagenesis; Risk assessment
MeSH headings : Animals; Antioxidant Response Elements / genetics; DNA Adducts / drug effects; DNA Adducts / genetics; DNA Damage / drug effects; DNA Damage / genetics; Dose-Response Relationship, Drug; Isotope Labeling; Mutagenesis / drug effects; Mutagenesis / genetics
TL;DR:
This review has focused on the endogenous exposome, the DNA damage that arises from the production of endogenous electrophilic molecules in the authors' cells, and provides quantitative data on endogenous DNA damage and its relationship to mutagenesis, with emphasis on when exogenous chemical exposures that produce identical DNA adducts to those arising from normal metabolism cause significant increases in total identical DNAAdducts.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2012 journal article
A New LC-MS/MS Method for the Quantification of Endogenous and Vinyl Chloride-Induced 7-(2-Oxoethyl)Guanine in Sprague–Dawley Rats
Chemical Research in Toxicology, 25(2), 391–399.
MeSH headings : Animals; Brain / metabolism; Chromatography, Liquid; DNA Adducts / analysis; DNA Adducts / metabolism; Guanine / analogs & derivatives; Guanine / analysis; Guanine / metabolism; Kidney / metabolism; Liver / metabolism; Lung / metabolism; Male; Rats; Rats, Sprague-Dawley; Spleen / metabolism; Tandem Mass Spectrometry; Testis / metabolism; Vinyl Chloride / pharmacokinetics
TL;DR:
A new sensitive LC-MS/MS method is reported to analyze the major DNA adduct formed by VC, 7-(2-oxoethylguanine) (7-OEG) to demonstrate for the first time that endogenous 7-Oeg was present in tissue DNA.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2012 journal article
The Warburg Effect Dictates the Mechanism of Butyrate-Mediated Histone Acetylation and Cell Proliferation
Molecular Cell, 48(4), 612–626.
MeSH headings : Acetylation; Butyrates / metabolism; Cell Proliferation; HCT116 Cells; HT29 Cells; Histones / metabolism; Humans; Models, Biological; Tumor Cells, Cultured
TL;DR:
It is demonstrated that metabolic transformation plays an important role in the development of cancer cells and that butyrate stimulated the proliferation of normal colonocytes and cancerous colonocytes when the Warburg effect was prevented from occurring.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2011 journal article
Debio 0507 primarily forms diaminocyclohexane-Pt-d(GpG) and -d(ApG) DNA adducts in HCT116 cells
Cancer Chemotherapy and Pharmacology, 69(3), 665–677.
author keywords: Debio 0507; Oxaliplatin; DNA adducts; LC/MS; UPLC/MS/MS
MeSH headings : Antineoplastic Agents / chemistry; Antineoplastic Agents / metabolism; Antineoplastic Agents / pharmacology; Cell Culture Techniques; Chromatography, High Pressure Liquid; DNA Adducts / chemistry; DNA Adducts / metabolism; Deoxyadenine Nucleotides / chemistry; Deoxyadenine Nucleotides / metabolism; Deoxyguanosine / chemistry; Deoxyguanosine / metabolism; Dinucleoside Phosphates / chemistry; Dinucleoside Phosphates / metabolism; HCT116 Cells; Humans; Organoplatinum Compounds / chemistry; Organoplatinum Compounds / metabolism; Organoplatinum Compounds / pharmacology; Oxaliplatin; Tandem Mass Spectrometry
TL;DR:
The data suggest that at equitoxic doses Debio 0507 and oxaliplatin form similar levels of dach-Pt-d(GpG) and dach(ApG) adducts, which suggests that the action mechanisms of Debio0507 andOxali Platin are similar at a cellular level.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2011 conference paper
Pseudo-SRM Conditions Improve Detection of N 2,3-ethenoguanine in DNA
Mutlu, E., Collins, L., Stout, M., Upton, P., Daye, L., Winsett, D., … Swenberg, J. (2011, June). Poster presented at the 59th American Society for Mass Spectrometry Conference, Denver, CO.
Event: 59th American Society for Mass Spectrometry Conference at Denver, CO on June 5-9, 2011
Source: NC State University Libraries
Added: September 9, 2021
2011 journal article
Tet Proteins Can Convert 5-Methylcytosine to 5-Formylcytosine and 5-Carboxylcytosine
Science, 333(6047), 1300–1303.
MeSH headings : 5-Methylcytosine / metabolism; Animals; Cell Line; Cytosine / analogs & derivatives; Cytosine / metabolism; DNA / metabolism; DNA Methylation; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Dioxygenases; Embryonic Stem Cells / metabolism; Humans; Mice; Oxidation-Reduction; Proto-Oncogene Proteins / genetics; Proto-Oncogene Proteins / metabolism; Recombinant Fusion Proteins / metabolism
TL;DR:
This study raises the possibility that DNA demethylation may occur through Tet-catalyzed oxidation followed by decarboxylation, and identifies two previously unknown cytosine derivatives in genomic DNA as the products of Tet proteins.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2010 journal article
Development and Application of an LC-MS/MS Method for the Detection of the Vinyl Chloride-Induced DNA Adduct N2,3-Ethenoguanine in Tissues of Adult and Weanling Rats Following Exposure to [13C2]-VC
Chemical Research in Toxicology, 23(9), 1485–1491.
TL;DR:
The use of stable isotope labeled VC permitted accurate estimates of the half-life of εG for the first time by comparing [(13)C(2)]-εG in adult rats with identically exposed animals euthanized 2, 4, or 8 weeks later, suggesting little or no active repair of this promutagenic adduct.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2010 journal article
Distribution of DNA Adducts Caused by Inhaled Formaldehyde Is Consistent with Induction of Nasal Carcinoma but Not Leukemia
Toxicological Sciences, 116(2), 441–451.
author keywords: formaldehyde; DNA adduct; DNA-DNA cross-link; cancer; leukemia
MeSH headings : Animals; Chromatography, High Pressure Liquid; DNA Adducts / analysis; Formaldehyde / metabolism; Formaldehyde / toxicity; Inhalation Exposure; Leukemia / chemically induced; Male; Nose Neoplasms / chemically induced; Rats; Rats, Inbred F344; Spectrometry, Mass, Electrospray Ionization
TL;DR:
This study provides strong evidence supporting a genotoxic and cytotoxic mode of action for the carcinogenesis of inhaled formaldehyde in respiratory nasal epithelium but does not support the biological plausibility that inhaling formaldehyde also causes leukemia.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2010 journal article
Structural Characterization of Formaldehyde-Induced Cross-Links Between Amino Acids and Deoxynucleosides and Their Oligomers
Journal of the American Chemical Society, 132(10), 3388–3399.
MeSH headings : Amino Acids / chemistry; Chromatography, High Pressure Liquid; Cross-Linking Reagents / chemistry; Deoxyadenosines / chemistry; Deoxycytidine / chemistry; Deoxyguanosine / chemistry; Formaldehyde / chemistry; Formic Acid Esters / chemistry; Nuclear Magnetic Resonance, Biomolecular / methods; Nucleosides / chemistry; Oligonucleotides / chemistry; Oligopeptides / chemistry; Spectroscopy, Fourier Transform Infrared; Tandem Mass Spectrometry / methods; Thymidine / chemistry
TL;DR:
Structural examination by NMR and mass spectrometry established that the cross-links between amino acids and single nucleosides involve a formaldehyde-derived methylene bridge, which will provide a basis for investigation of the characteristics and properties of DPCs formed in vivo.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2009 journal article
Accurate quantitation of standard peptides used for quantitative proteomics
Proteomics, 9(15), 3939–3944.
author keywords: Peptide standards; Quality control; Quantitative analysis
MeSH headings : Chromatography, Liquid / economics; Chromatography, Liquid / methods; Peptides / analysis; Proteomics / economics; Proteomics / methods; Sensitivity and Specificity; Tandem Mass Spectrometry / economics; Tandem Mass Spectrometry / methods
TL;DR:
The presented method utilizes ultra performance LC‐ MS/MS (UPLC‐MS/MS) to accurately quantify peptide standards at concentrations of 0.1–10 μM, and the high sensitivity of the method minimizes the amounts required.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2009 journal article
Analysis of 8-oxo-7,8-dihydro-2′-deoxyguanosine by ultra high pressure liquid chromatography–heat assisted electrospray ionization–tandem mass spectrometry
Journal of Chromatography B, 878(3-4), 375–380.
author keywords: 8-Oxo-7,8-dihydro-2 '-deoxyguanosine; Oxidative stress; LC-MS
MeSH headings : 8-Hydroxy-2'-Deoxyguanosine; Adult; Animals; Chromatography, High Pressure Liquid / methods; DNA / metabolism; Deoxyguanosine / analogs & derivatives; Deoxyguanosine / analysis; Freezing; HeLa Cells; Hot Temperature; Humans; Hydrogen Peroxide / pharmacology; Lymphocytes / drug effects; Lymphocytes / metabolism; Middle Aged; Rats; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization / methods; Tandem Mass Spectrometry / methods; Young Adult
TL;DR:
The present study demonstrates that 8-oxo-dG can be measured reliably and accurately when appropriate precautions are taken and suggests that the present method is well suited for application to molecular toxicology and epidemiology studies investigating the role of oxidative stress.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2009 journal article
Comparison of three oxidative stress biomarkers in a sample of healthy adults
Biomarkers, 14(8), 587–595.
author keywords: Oxidation DNA damage; lipid peroxidation; 8-hydroxy-2-deoxyguanosine; comet assay; isoprostanes
MeSH headings : 8-Hydroxy-2'-Deoxyguanosine; Adult; Biomarkers / analysis; Biomarkers / metabolism; Comet Assay; DNA Damage; DNA-Formamidopyrimidine Glycosylase; Deoxyguanosine / analogs & derivatives; Deoxyguanosine / metabolism; F2-Isoprostanes / metabolism; Female; Humans; Lipid Peroxidation; Male; Middle Aged; Oxidative Stress
TL;DR:
Results are informative for researchers seeking to compare results pertaining to oxidative stress across studies and/or assessment methods in healthy disease-free populations and additional validation studies are necessary to establish accuracy and comparability across oxidative stress biomarkers.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2009 journal article
Development of an Ultraperformance Liquid Chromatography/Mass Spectrometry Method To Quantify Cisplatin 1,2 Intrastrand Guanine−Guanine Adducts
Chemical Research in Toxicology, 22(5), 905–912.
MeSH headings : Animals; Carboplatin / chemistry; Cattle; Cell Line, Tumor; Chromatography, High Pressure Liquid / methods; Cisplatin / chemistry; DNA / chemistry; Female; Guanine / chemistry; Mice; Mice, Inbred C57BL; Ribonucleosides / chemistry; Tandem Mass Spectrometry / methods
TL;DR:
An ultraperformance liquid chromatography tandem mass spectrometry assay for quantification of 1,2 guanine-guanine intrastrand cisplatin adducts, using (15)N(10) CP-d(GpG) as an internal standard, was developed and suggested that this method is suitable for in vitro and in vivo assessment.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2009 journal article
Liquid chromatography electrospray ionization tandem mass spectrometry analysis method for simultaneous detection of trichloroacetic acid, dichloroacetic acid, S-(1,2-dichlorovinyl)glutathione and S-(1,2-dichlorovinyl)-L-cysteine
Toxicology, 262(3), 230–238.
author keywords: Trichloroethylene metabolites; HPLC-ESI-MS/MS; Dichloroacetic acid; Trichloroacetic acid; S-(1,2-dichlorovinyl)-L-cysteine; S-(1,2-dichlorovinyl)glutathione; HILIC
MeSH headings : Animals; Chromatography, High Pressure Liquid / methods; Cysteine / analogs & derivatives; Cysteine / blood; Dichloroacetic Acid / blood; Glutathione / analogs & derivatives; Glutathione / blood; Male; Mice; Spectrometry, Mass, Electrospray Ionization / methods; Tandem Mass Spectrometry / methods; Trichloroacetic Acid / blood; Trichloroethylene / metabolism
TL;DR:
This method was applied successfully to measure all 4 TCE metabolites in as little as 50 microl of serum from mice orally exposed to TCE, opening new opportunities to increase throughput and decrease number of animals required for mechanistic studies on TCE in rodents.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2009 journal article
Pharmacokinetic analysis of trichloroethylene metabolism in male B6C3F1 mice: Formation and disposition of trichloroacetic acid, dichloroacetic acid, S-(1,2-dichlorovinyl)glutathione and S-(1,2-dichlorovinyl)-l-cysteine
Toxicology and Applied Pharmacology, 238(1), 90–99.
author keywords: Trichloroethylene; Metabolism; Pharmacokinetics; Trichloroacetic acid; Dichloroacetic acid; S-(1,2-dichlorovinyl)glutathione; S-(1,2-dichlorovinyl)-L-cysteine
MeSH headings : Administration, Oral; Animals; Biological Availability; Carcinogens / pharmacokinetics; Cysteine / analogs & derivatives; Cysteine / pharmacokinetics; Dichloroacetic Acid / pharmacokinetics; Glutathione / analogs & derivatives; Glutathione / metabolism; Glutathione / pharmacokinetics; Half-Life; Male; Mice; Models, Biological; Oxidation-Reduction; Risk Assessment; Time Factors; Trichloroacetic Acid / pharmacokinetics; Trichloroethylene / pharmacokinetics
TL;DR:
In B6C3F1 mice, oxidative metabolites are formed in much greater quantities than glutathione-conjugative metabolites, and TCE-oxide is the most likely source of DCA, while most of DCVG is converted into DCVC.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2008 journal article
A mass spectrometric method to simultaneously measure a biomarker and dilution marker in exhaled breath condensate
Rapid Communications in Mass Spectrometry, 22(5), 701–705.
MeSH headings : Adenosine / analysis; Adenosine / metabolism; Adenosine Monophosphate / analysis; Adenosine Monophosphate / metabolism; Biomarkers / analysis; Biomarkers / metabolism; Breath Tests / methods; Bronchi / metabolism; Child; Chromatography, High Pressure Liquid; Cystic Fibrosis / metabolism; Exhalation; Humans; Prospective Studies; Spectrometry, Mass, Electrospray Ionization / methods; Tandem Mass Spectrometry / methods; Urea / analysis; Urea / metabolism
TL;DR:
It is demonstrated that mass spectrometry can be used successfully in EBC analysis to simultaneously detect a biomarker for airway inflammation and control for variable dilution.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2008 journal article
Formaldehyde-Induced Histone Modifications in Vitro
Chemical Research in Toxicology, 21(8), 1586–1593.
MeSH headings : Binding Sites; Chromatography, High Pressure Liquid; Cross-Linking Reagents / chemistry; Cross-Linking Reagents / metabolism; DNA / drug effects; DNA Damage; Formaldehyde / chemistry; Formaldehyde / metabolism; Histones / chemistry; Histones / metabolism; In Vitro Techniques; Lysine / chemistry; Lysine / metabolism; Peptide Fragments / chemistry; Peptide Mapping; Protein Binding; Protein Structure, Tertiary; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tandem Mass Spectrometry
TL;DR:
The observation that only lysine residues without post-translational modification (PTM) can be attacked by formaldehyde indicates that PTM blocks the reaction betweenlysine and formaldehyde.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
2005 conference paper
Use of QuEChERS for residue determination in unusual matrices
Collins, L. B. (2005, April 27). Presented at the 19th annual meeting of the Southeast Regional Section of the AOAC International, Atlanta, GA.
Event: 19th annual meeting of the Southeast Regional Section of the AOAC International at Atlanta, GA on April 27, 2005
Source: NC State University Libraries
Added: September 9, 2021
1998 conference paper
HPLC/UV method for determination of Tepraloxydim major metabolites in soil and sediment
Saha, M., Harrison, B., & Collins, L. B. (1998, August 23). Poster presented at the 216th American Chemical Society (ACS) National Meeting, Boston, MA.
Event: 216th American Chemical Society (ACS) National Meeting at Boston, MA on August 23-27, 1998
Source: NC State University Libraries
Added: September 9, 2021
1994 journal article
Interference by pH and Ca2+ ions during measurements of catecholamine release in slices of rat amygdala with fast-scan cyclic voltammetry
Journal of Neuroscience Methods, 52(1), 1–10.
author keywords: STRIATUM; BASOLATERAL AMYGDALA; DOPAMINE RELEASE; FAST-SCAN CYCLIC VOLTAMMETRY; L-DOPA; ION-SELECTIVE MICROELECTRODE; CALCIUM; PH
MeSH headings : Amygdala / metabolism; Animals; Calcium / physiology; Carbon; Catecholamines / deficiency; Catecholamines / metabolism; Caudate Nucleus / metabolism; Dopamine / pharmacology; Electrophysiology / instrumentation; Electrophysiology / methods; Hydrogen-Ion Concentration; In Vitro Techniques; Levodopa / pharmacology; Male; Microelectrodes; Rats; Rats, Sprague-Dawley; Time Factors
TL;DR:
Fast-scan cyclic voltammetry at carbon-fiber microelectrodes was used to investigate catecholamine release and uptake induced by local electrical stimulation of rat brain slices containing the basolateral amygdaloid nucleus, and the observed release is proportionately smaller than that of the striatum.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
1993 journal article
Evoked Extracellular Dopamine In Vivo in the Medial Prefrontal Cortex
Journal of Neurochemistry, 61(2), 637–647.
MeSH headings : Animals; Dopamine / metabolism; Electric Stimulation; Electrochemistry; Extracellular Space / metabolism; Kinetics; Male; Methyltyrosines / pharmacology; Microelectrodes; Neurons / metabolism; Norepinephrine / metabolism; Prefrontal Cortex / drug effects; Prefrontal Cortex / metabolism; Rats; Rats, Sprague-Dawley; alpha-Methyltyrosine
TL;DR:
Despite the sparse dopaminergic innervation and the presence of more dense noradrenergic and serotonergic innervations overall in the medial prefrontal cortex, the measurement of extracellular dopamine was achieved by selective recording in dopamine‐rich terminal fields and selective activation of ascending dopamine neurons.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 5, 2021
Employment
Updated: April 14th, 2020 17:29
2020 - present
North Carolina State University Raleigh, North Carolina, US
Research Assistant
METRIC
2006 - 2020
University of North Carolina at Chapel Hill Chapel Hill, NC, US
Research Specialist
Environmental Sciences and Engineering
1999 - 2005
Mississippi State Chemical Laboratory Starkville, Mississippi, US
Research Associate
1994 - 1999
BASF Corporation Research Triangle Park, North Carolina, US
Associate Chemist
Agricultural Products Unit
1993 - 1994
Magellan Laboratories, Inc. Morrisville, North Carolina, US
Analyst
Education
Updated: August 29th, 2021 11:53
2000 - 2004
Mississippi State University Starkville, MS, US
MBA
1989 - 1993
University of North Carolina at Chapel Hill Chapel Hill, North Carolina, US
BS
Citation Index includes data from a number of different sources. If you have questions about the sources of data in the Citation Index or need a set of data which is free to re-distribute, please contact us.
Certain data included herein are derived from the Web of Science© and InCites© (2024) of Clarivate Analytics. All rights reserved. You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.