@article{mellor_nordberg_huebner_mohiti-asli_taylor_efird_oxford_spang_shirwaiker_loboa_2020, title={Investigation of multiphasic 3D-bioplotted scaffolds for site-specific chondrogenic and osteogenic differentiation of human adipose-derived stem cells for osteochondral tissue engineering applications}, volume={108}, ISSN={["1552-4981"]}, DOI={10.1002/jbm.b.34542}, abstractNote={Abstract}, number={5}, journal={JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS}, author={Mellor, Liliana F. and Nordberg, Rachel C. and Huebner, Pedro and Mohiti-Asli, Mahsa and Taylor, Michael A. and Efird, William and Oxford, Julia T. and Spang, Jeffrey T. and Shirwaiker, Rohan A. and Loboa, Elizabeth G.}, year={2020}, month={Jul}, pages={2017–2030} }
 @article{mellor_steward_nordberg_taylor_loboa_2017, title={Comparison of simulated microgravity and hydrostatic pressure for chondrogenesis of hASC}, volume={88}, number={4}, journal={Aerospace Medicine and Human Performance}, author={Mellor, L. F. and Steward, A. J. and Nordberg, R. C. and Taylor, M. A. and Loboa, E. G.}, year={2017}, pages={377–384} }
 @article{mehendale_mellor_taylor_loboa_shirwaiker_2017, title={Effects of 3D-bioplotted polycaprolactone scaffold geometry on human adipose-derived stem cell viability and proliferation}, volume={23}, ISSN={["1758-7670"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85019575149&partnerID=MN8TOARS}, DOI={10.1108/rpj-03-2016-0035}, abstractNote={
Purpose
This study aims to investigate the effect of three-dimensional (3D)- bioplotted polycaprolactone (PCL) scaffold geometry on the biological and mechanical characteristics of human adipose-derived stem cell (hASC) seeded constructs.
}, number={3}, journal={RAPID PROTOTYPING JOURNAL}, author={Mehendale, Saahil V. and Mellor, Liliana F. and Taylor, Michael A. and Loboa, Elizabeth G. and Shirwaiker, Rohan A.}, year={2017}, pages={534–542} }
 @article{mellor_huebner_cai_mohiti-asli_taylor_spang_shirwaiker_loboa_2017, title={Fabrication and Evaluation of Electrospun, 3D-Bioplotted, and Combination of Electrospun/3D-Bioplotted Scaffolds for Tissue Engineering Applications}, volume={2017}, ISSN={["2314-6141"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85018911833&partnerID=MN8TOARS}, DOI={10.1155/2017/6956794}, abstractNote={Electrospun scaffolds provide a dense framework of nanofibers with pore sizes and fiber diameters that closely resemble the architecture of native extracellular matrix. However, it generates limited three-dimensional structures of relevant physiological thicknesses. 3D printing allows digitally controlled fabrication of three-dimensional single/multimaterial constructs with precisely ordered fiber and pore architecture in a single build. However, this approach generally lacks the ability to achieve submicron resolution features to mimic native tissue. The goal of this study was to fabricate and evaluate 3D printed, electrospun, and combination of 3D printed/electrospun scaffolds to mimic the native architecture of heterogeneous tissue. We assessed their ability to support viability and proliferation of human adipose derived stem cells (hASC). Cells had increased proliferation and high viability over 21 days on all scaffolds. We further tested implantation of stacked-electrospun scaffold versus combined electrospun/3D scaffold on a cadaveric pig knee model and found that stacked-electrospun scaffold easily delaminated during implantation while the combined scaffold was easier to implant. Our approach combining these two commonly used scaffold fabrication technologies allows for the creation of a scaffold with more close resemblance to heterogeneous tissue architecture, holding great potential for tissue engineering and regenerative medicine applications of osteochondral tissue and other heterogeneous tissues.}, journal={BIOMED RESEARCH INTERNATIONAL}, author={Mellor, Liliana F. and Huebner, Pedro and Cai, Shaobo and Mohiti-Asli, Mahsa and Taylor, Michael A. and Spang, Jeffrey and Shirwaiker, Rohan A. and Loboa, Elizabeth G.}, year={2017} }
 @article{neupane_jin_mellor_loboa_ligler_wang_2015, title={Continuous-wave stimulated emission depletion microscope for imaging actin cytoskeleton in fixed and live cells}, volume={15}, number={9}, journal={Sensors (Basel, Switzerland)}, author={Neupane, B. and Jin, T. and Mellor, L. F. and Loboa, E. G. and Ligler, F. S. and Wang, G. F.}, year={2015}, pages={24178–24190} }
 @article{mellor_mohiti-asli_williams_kannan_dent_guilak_loboa_2015, title={Extracellular Calcium Modulates Chondrogenic and Osteogenic Differentiation of Human Adipose-Derived Stem Cells: A Novel Approach for Osteochondral Tissue Engineering Using a Single Stem Cell Source}, volume={21}, ISSN={["1937-335X"]}, DOI={10.1089/ten.tea.2014.0572}, abstractNote={We have previously shown that elevating extracellular calcium from a concentration of 1.8 to 8 mM accelerates and increases human adipose-derived stem cell (hASC) osteogenic differentiation and cell-mediated calcium accretion, even in the absence of any other soluble osteogenic factors in the culture medium. However, the effects of elevated calcium on hASC chondrogenic differentiation have not been reported. The goal of this study was to determine the effects of varied calcium concentrations on chondrogenic differentiation of hASC. We hypothesized that exposure to elevated extracellular calcium (8 mM concentration) in a chondrogenic differentiation medium (CDM) would inhibit chondrogenesis of hASC when compared to basal calcium (1.8 mM concentration) controls. We further hypothesized that a full osteochondral construct could be engineered by controlling local release of calcium to induce site-specific chondrogenesis and osteogenesis using only hASC as the cell source. Human ASC was cultured as micromass pellets in CDM containing transforming growth factor-β1 and bone morphogenetic protein 6 for 28 days at extracellular calcium concentrations of either 1.8 mM (basal) or 8 mM (elevated). Our findings indicated that elevated calcium induced osteogenesis and inhibited chondrogenesis in hASC. Based on these findings, stacked polylactic acid nanofibrous scaffolds containing either 0% or 20% tricalcium phosphate (TCP) nanoparticles were electrospun and tested for site-specific chondrogenesis and osteogenesis. Histological assays confirmed that human ASC differentiated locally to generate calcified tissue in layers containing 20% TCP, and cartilage in the layers with no TCP when cultured in CDM. This is the first study to report the effects of elevated calcium on chondrogenic differentiation of hASC, and to develop osteochondral nanofibrous scaffolds using a single cell source and controlled calcium release to induce site-specific differentiation. This approach holds great promise for osteochondral tissue engineering using a single cell source (hASC) and single scaffold.}, number={17-18}, journal={TISSUE ENGINEERING PART A}, author={Mellor, Liliana F. and Mohiti-Asli, Mahsa and Williams, John and Kannan, Arthi and Dent, Morgan R. and Guilak, Farshid and Loboa, Elizabeth G.}, year={2015}, month={Sep}, pages={2323–2333} }