Works (102)
Updated: April 3rd, 2024 19:43
2023 journal article
Cassava begomovirus species diversity changes during plant vegetative cycles
Frontiers in Microbiology, 14.
author keywords: plant virus; Cassava (Manihot esculenta); vegetative (asexual) propagation; vector transmission; whitefly (Bemisia tabaci)
TL;DR:
The results suggest that whitefly transmission and vegetative transmission lead to different outcomes for ACMV and EACMV-like viruses.
(via Semantic Scholar)
www.frontiersin.org (publisher PDF)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: July 3, 2023
2023 journal article
Genome segment ratios change during whitefly transmission of two bipartite cassava mosaic begomoviruses
Scientific Reports, 13(1).
MeSH headings : Animals; Begomovirus / genetics; Manihot; Hemiptera; Plants; Vegetables; Plant Diseases
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 7, 2023
2023 journal article
SEGS-1 a cassava genomic sequence increases the severity of African cassava mosaic virus infection in <i>Arabidopsis</i> thaliana
FRONTIERS IN PLANT SCIENCE, 14.
author keywords: SEGS-1; begomovirus; ACMV; Arabidopsis thaliana; cassava
TL;DR:
It is demonstrated that SEGS-1 can function in a heterologous host to increase disease severity in Arabidopsis thaliana plants that are co-inoculated with African cassava mosaic virus (ACMV) and SEGs-1 sequences.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: January 2, 2024
2022 journal article
An experimental strategy for preparing circular ssDNA virus genomes for next-generation sequencing
JOURNAL OF VIROLOGICAL METHODS, 300.
author keywords: Phi29; EquiPhi29; MiSeq; Whiteflies; Viral DNA sequencing
MeSH headings : DNA Viruses / genetics; DNA, Circular / genetics; DNA, Viral / genetics; Genome, Viral; High-Throughput Nucleotide Sequencing
TL;DR:
An improved protocol for the enhanced amplification and enrichment of begomovirus DNA for use in next generation sequencing of the viral genomes is presented and can be used to examine the viral DNA as it moves from host to vector, thus producing valuable information for viral DNA population studies.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: March 7, 2022
2022 journal article
Early detection of plant virus infection using multispectral imaging and spatial-spectral machine learning
SCIENTIFIC REPORTS, 12(1).
MeSH headings : Disease Resistance; Early Diagnosis; Machine Learning; Manihot / virology; Photometry / instrumentation; Photometry / methods; Plant Diseases / virology; Plant Viruses / genetics; Plant Viruses / pathogenicity; Potyviridae / pathogenicity; RNA, Viral; Spectrophotometry / instrumentation; Spectrophotometry / methods; Virus Diseases / diagnosis
TL;DR:
A handheld active multispectral imaging (A-MSI) device combined with machine learning for early detection of CBSD in real-time is developed and has the potential to increase farmers’ access to healthy planting materials and reduce losses due toCBSD in Africa.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 21, 2022
2022 journal article
Vector acquisition and co-inoculation of two plant viruses influences transmission, infection, and replication in new hosts
SCIENTIFIC REPORTS, 12(1).
MeSH headings : Animals; Coinfection; Disease Vectors; Plant Viruses; Vaccination; Geminiviridae
TL;DR:
Findings highlight the complex nature of vector-virus-plant interactions that influence the spread and replication of viruses as single and co-infections.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 19, 2022
2021 journal article
A New Type of Satellite Associated with Cassava Mosaic Begomoviruses
JOURNAL OF VIROLOGY, 95(21).
author keywords: Arabidopsis; begomovirus; cassava mosaic disease; SEGS
MeSH headings : Begomovirus / classification; Begomovirus / genetics; Begomovirus / isolation & purification; Begomovirus / pathogenicity; DNA, Viral / genetics; Genome, Viral; Manihot / virology; Mutation; Phylogeny; Plant Diseases / virology; Recombination, Genetic; Satellite Viruses / classification; Satellite Viruses / genetics; Satellite Viruses / isolation & purification; Satellite Viruses / pathogenicity; Tobacco / virology
TL;DR:
SEGS-2, which was originally isolated from cassava crops displaying severe and atypical CMD symptoms in Tanzanian fields, is a novel begomovirus satellite that can compromise the development of durable CMD resistance.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 1, 2021
2021 journal article
A calmodulin-binding transcription factor links calcium signaling to antiviral RNAi defense in plants
CELL HOST & MICROBE, 29(9), 1393-+.
MeSH headings : Argonaute Proteins / genetics; Argonaute Proteins / metabolism; Calcium / metabolism; Calcium Signaling / genetics; Calmodulin / metabolism; Cucumovirus / pathogenicity; Endonucleases / metabolism; Geminiviridae / pathogenicity; MicroRNAs / metabolism; Plant Diseases / prevention & control; Plant Diseases / virology; Plants; Potyviridae / pathogenicity; RNA Interference / physiology; RNA, Small Interfering / genetics; RNA-Dependent RNA Polymerase / metabolism; Ribonuclease III / genetics; Ribonuclease III / metabolism; Tobacco / genetics; Tobacco / virology; Transcription Factors / metabolism
TL;DR:
It is shown that wounding to Nicotiana benthamiana cells during virus intrusion activates RNAi-related gene expression through calcium signaling, linking Ca2+ signaling to RNAi and revealing versatility of host antiviral defense and viral counter-defense.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 26, 2021
2021 journal article
A protocol for genome-wide analysis of DNA replication timing in intact root tips
Methods in Molecular Biology Series.
Source: NC State University Libraries
Added: December 18, 2020
2021 journal article
Loss of Small-RNA-Directed DNA Methylation in the Plant Cell Cycle Promotes Germline Reprogramming and Somaclonal Variation
CURRENT BIOLOGY, 31(3), 591-+.
MeSH headings : Arabidopsis / genetics; Arabidopsis / metabolism; Cell Cycle; Cytosine; DNA Methylation; DNA, Plant; Gene Expression Regulation, Plant; Germ Cells / metabolism; Histones / genetics; Histones / metabolism; Plants / metabolism; RNA, Small Interfering / genetics
TL;DR:
Comparative analysis with methylomes of various tissues and cell types suggests that loss of small-RNA-directed non-CG methylation during DNA replication promotes germline reprogramming and epigenetic variation in plants propagated as clones.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: December 4, 2020
2021 journal article
Population diversity of cassava mosaic begomoviruses increases over the course of serial vegetative propagation
Journal of General Virology, 102(7).
author keywords: cassava mosaic begomoviruses; vegetative propagation; viral diversity
MeSH headings : Base Sequence; Begomovirus / genetics; Begomovirus / physiology; Codon; DNA, Intergenic; DNA, Viral / genetics; Evolution, Molecular; Genetic Variation; Genome, Viral; Manihot / growth & development; Manihot / virology; Mutation; Plant Diseases / virology; Polymorphism, Single Nucleotide; Satellite Viruses / genetics; Satellite Viruses / physiology; Sequence Deletion; Temperature; Viral Proteins / genetics
TL;DR:
It is found that vegetative propagation had a significant effect on viral nucleotide diversity, while temperature and a satellite DNA did not have measurable impacts in this study.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 16, 2021
2021 journal article
SnRK1: a versatile plant protein kinase that limits geminivirus infection
CURRENT OPINION IN VIROLOGY, 47, 18–24.
MeSH headings : Disease Resistance; Geminiviridae / genetics; Geminiviridae / metabolism; Geminiviridae / physiology; Host-Pathogen Interactions; Phosphorylation; Plant Diseases / virology; Plant Proteins / genetics; Plant Proteins / metabolism; Protein Kinases / genetics; Protein Kinases / metabolism; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR:
Data suggest that SnRK1 is involved in the co-evolution of plant hosts and geminiviruses.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: May 17, 2021
2020 journal article
A plant DNA virus replicates in the salivary glands of its insect vector via recruitment of host DNA synthesis machinery
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 117(29), 16928–16937.
author keywords: DNA synthesis machinery; insect vector; plant DNA virus; replication; salivary glands
MeSH headings : Animals; Begomovirus / genetics; Begomovirus / physiology; DNA Polymerase III / genetics; DNA Polymerase III / metabolism; Gossypium / parasitology; Gossypium / virology; Hemiptera / pathogenicity; Hemiptera / virology; Host-Pathogen Interactions; Insect Proteins / genetics; Insect Proteins / metabolism; Insect Vectors / pathogenicity; Insect Vectors / virology; Salivary Glands / metabolism; Salivary Glands / virology; Virus Replication
TL;DR:
It is found that TYLCV induces DNA synthesis machinery, proliferating cell nuclear antigen (PCNA) and DNA polymerase δ (Polδ), to establish a replication-competent environment in whiteflies, and that replication is mainly in the salivary glands.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 10, 2020
2020 journal article
Arabidopsis DNA Replication Initiates in Intergenic, AT-Rich Open Chromatin(1)([OPEN])
PLANT PHYSIOLOGY, 183(1), 206–220.
MeSH headings : Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Chromatin / metabolism; DNA Replication / genetics; DNA Replication / physiology; DNA, Plant / metabolism; DNA, Plant / physiology; Replication Origin / genetics; Replication Origin / physiology
TL;DR:
A new functional assay to label and map very early replicating loci that must, by definition, include at least a subset of replication origins, and indicates that IRs are associated with accessible chromatin.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 15, 2020
2020 journal article
Comparing DNA replication programs reveals large timing shifts at centromeres of endocycling cells in maize roots
PLOS GENETICS, 16(10).
MeSH headings : Cell Nucleus / drug effects; Cell Nucleus / genetics; Centromere / drug effects; Centromere / genetics; DNA Replication / drug effects; DNA Replication / genetics; DNA Replication Timing / drug effects; DNA Replication Timing / genetics; DNA, Plant / drug effects; DNA, Plant / genetics; Deoxyuridine / analogs & derivatives; Deoxyuridine / pharmacology; Endocytosis / drug effects; Meristem / drug effects; Meristem / genetics; Mitosis / drug effects; Mitosis / genetics; Nucleosomes / drug effects; Plant Roots / drug effects; Plant Roots / genetics; Plant Roots / growth & development; S Phase / genetics; Zea mays / genetics; Zea mays / growth & development
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 9, 2020
2020 journal article
Cotton leaf curl Multan virus beta C1 Protein Induces Autophagy by Disrupting the Interaction of Autophagy-Related Protein 3 with Glyceraldehyde-3-Phosphate Dehydrogenases([OPEN])
PLANT CELL, 32(4), 1124–1135.
MeSH headings : Autophagy; Autophagy-Related Proteins / metabolism; Begomovirus / metabolism; Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism; Plant Proteins / metabolism; Protein Binding; Tobacco / metabolism; Tobacco / ultrastructure; Tobacco / virology; Vacuoles / metabolism; Vacuoles / ultrastructure; Viral Proteins / metabolism
TL;DR:
It is reported that the βC1 protein of Cotton leaf curl Multan betasatellite (CLCuMuB) interacts with cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPC), a negative autophagic regulator, to induce autophagy in Nicotiana benthamiana.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: May 18, 2020
2020 journal article
Deeply Sequenced Infectious Clones of Key Cassava Begomovirus Isolates from Cameroon
MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 9(46).
TL;DR:
This work deeply sequenced two pairs of widely used infectious clones (4 plasmids) of the bipartite begomoviruses African cassava mosaic virus (ACMV) and East AfricanCassava mosaic Cameroon virus (EACMCV).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: January 11, 2021
2019 journal article
Geminiviral V2 Protein Suppresses Transcriptional Gene Silencing through Interaction with AGO4
JOURNAL OF VIROLOGY, 93(6).
author keywords: Argonaute 4; CLCuMuV; RdDM pathway; TGS; V2
MeSH headings : Begomovirus / genetics; DNA Methylation / genetics; DNA, Viral / genetics; Geminiviridae / genetics; Gene Silencing / physiology; Host-Pathogen Interactions / genetics; Plant Diseases / virology; Tobacco / virology; Transcription, Genetic / genetics; Viral Proteins / genetics
TL;DR:
This work provides the first evidence that a geminiviral protein is able to directly target core RdDM components to counter RdDM-mediated TGS antiviraldefense in plants, which extends the current understanding of viral counters to host antiviral defense.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 25, 2019
2018 chapter
Cassava viruses – host jumps, virus recombination, spread in plant material
In A. Records & J. Ristaino (Eds.), Emerging Plant Diseases and Global Food Security. St. Paul, MN: APS Press.
Ed(s): A. Records & J. Ristaino
Source: NC State University Libraries
Added: September 24, 2020
2018 article
Chromatin structure profile data from DNS-seq: Differential nuclease sensitivity mapping of four reference tissues of B73 maize (Zea mays L)
Turpin, Z. M., Vera, D. L., Savadel, S. D., Lung, P.-Y., Wear, E. E., Mickelson-Young, L., … Bass, H. W. (2018, October). DATA IN BRIEF, Vol. 20, pp. 358–363.
TL;DR:
Data from Next-Generation Sequencing of differential micrococcal nuclease digestions of formaldehyde-crosslinked chromatin in selected tissues of maize (Zea mays) inbred line B73 are presented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 3, 2018
2018 journal article
Cotton Leaf Curl Multan virus C4 protein suppresses both transcriptional and post-transcriptional gene silencing by interacting with SAM synthetase
PLOS Pathogens, 14(8), e1007282.
Ed(s): S. Dinesh-Kumar
MeSH headings : Begomovirus / metabolism; Begomovirus / pathogenicity; Down-Regulation / genetics; Gene Expression Regulation, Plant; Gene Silencing; Host-Pathogen Interactions / genetics; Methionine Adenosyltransferase / genetics; Methionine Adenosyltransferase / metabolism; Plants, Genetically Modified; Protein Binding; RNA Interference; Tobacco / genetics; Tobacco / metabolism; Transcription, Genetic; Viral Proteins / metabolism; Viral Proteins / physiology
TL;DR:
It is reported that Cotton Leaf Curl Multan virus (CLCuMuV) C4 protein interacts with S-adenosyl methionine synthetase (SAMS), a core enzyme in the methyl cycle, and inhibits SAMS enzymatic activity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
2018 journal article
Diacylglycerol acyltransferase 1 is activated by phosphatidate and inhibited by SnRK1-catalyzed phosphorylation
PLANT JOURNAL, 96(2), 287–299.
author keywords: triacylglycerol biosynthesis; DGAT; Brassica napus; biochemical regulation; allostery
MeSH headings : Acyl Coenzyme A / metabolism; Brassica napus / enzymology; Brassica napus / genetics; Carbohydrate Metabolism; Catalysis; Diacylglycerol O-Acyltransferase / genetics; Diacylglycerol O-Acyltransferase / metabolism; Energy Metabolism; Homeostasis; Lipids / physiology; Phosphatidic Acids / metabolism; Phosphorylation; Plant Proteins / genetics; Plant Proteins / metabolism; Protein Serine-Threonine Kinases / genetics; Protein Serine-Threonine Kinases / metabolism; Triglycerides / biosynthesis
TL;DR:
Phosphatidate (PA) was identified as a feed-forward activator of BnaDGAT1, enabling the final enzyme in the Kennedy pathway to adjust to the incoming flow of carbon leading to TAG, indicating that PA facilitates the transition of the enzyme into the more active state.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 22, 2018
2018 journal article
Genome-Wide Analysis of the Arabidopsis Replication Timing Program
PLANT PHYSIOLOGY, 176(3), 2166–2185.
MeSH headings : Arabidopsis / genetics; Chromatin / genetics; Chromatin / metabolism; Chromosomes, Plant; DNA Replication Timing; DNA Transposable Elements; Flow Cytometry; Genome, Plant; Genome-Wide Association Study; S Phase / genetics; Sequence Analysis, DNA / methods
TL;DR:
The thymidine analog, 5-ethynyl-2′-deoxyuridine, is used in combination with flow sorting and Repli-Seq to describe the genome-wide replication timing program for Arabidopsis (Arabidopsis thaliana) Col-0 suspension cells, and genomic regions that replicate predominantly during early, mid, and late S phase are identified.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2018 journal article
Lanai: A small, fast growing tomato variety is an excellent model system for studying geminiviruses
JOURNAL OF VIROLOGICAL METHODS, 256, 89–99.
author keywords: Florida lanai; Tomato; Geminiviruses; Symptoms; qPCR; Ploidy; Seed transmission
MeSH headings : Analysis of Variance; Geminiviridae / genetics; Genome, Viral; Solanum lycopersicum / virology; Phenotype; Plant Diseases / virology; Ploidies
TL;DR:
Florida Lanai is a tomato variety suitable for virus-host interaction studies and an excellent choice for comparing geminivirus infections.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2018 journal article
Sucrose Nonfermenting 1-Related Protein Kinase 1 Phosphorylates a Geminivirus Rep Protein to Impair Viral Replication and Infection
PLANT PHYSIOLOGY, 178(1), 372–389.
MeSH headings : Amino Acid Sequence; Begomovirus / genetics; Begomovirus / metabolism; Begomovirus / physiology; Host-Pathogen Interactions; Solanum lycopersicum / enzymology; Solanum lycopersicum / virology; Mutation; Phosphorylation; Plant Diseases / virology; Plant Proteins / genetics; Plant Proteins / metabolism; Protein Binding; Protein Domains; Protein Serine-Threonine Kinases / genetics; Protein Serine-Threonine Kinases / metabolism; Sequence Homology, Amino Acid; Serine / genetics; Serine / metabolism; Viral Proteins / chemistry; Viral Proteins / genetics; Viral Proteins / metabolism; Virus Replication
TL;DR:
It is established that SnRK1 phosphorylates Rep and interferes with geminivirus replication and infection, underscoring the emerging role for SnRk1 in the host defense response against plant pathogens.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 16, 2018
2017 journal article
A VIGS screen identifies immunity in the Arabidopsis Pla-1 accession to viruses in two different genera of the Geminiviridae
PLANT JOURNAL, 92(5), 796–807.
author keywords: Arabidopsis; BCTV; CaLCuV; geminivirus; gip-1; immunity; Pla-1; VIGS
MeSH headings : Arabidopsis / virology; Geminiviridae / immunology; Gene Silencing; Plant Diseases / immunology; Plant Diseases / virology; Plant Immunity; Quantitative Trait Loci / genetics
TL;DR:
The recessive nature of resistance to CaLCuV and the lack of obvious candidate genes near the gip‐1 locus suggest that a novel resistance gene(s) confers immunity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2017 journal article
Foundational and Translational Research Opportunities to Improve Plant Health
Molecular Plant-Microbe Interactions, 30(7), 515–516.
MeSH headings : Agriculture / methods; Biotechnology / methods; Climate Change; Crops, Agricultural / growth & development; Crops, Agricultural / microbiology; Crops, Agricultural / parasitology; Food Supply; Humans; Plant Diseases / microbiology; Plant Diseases / parasitology; Translational Research, Biomedical / methods
TL;DR:
The white paper reports the deliberations of a workshop focused on biotic challenges to plant health held in Washington, D.C. in September 2016, and concludes that data-driven, informed decisions based on knowledge of the variation ofBiotic challenges and of natural and synthetic variation in crop plants will enable deployment of durable interventions throughout the world.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
2017 journal article
Genomic Analysis of the DNA Replication Timing Program during Mitotic S Phase in Maize (Zea mays) Root Tips
PLANT CELL, 29(9), 2126–2149.
MeSH headings : Base Sequence; Chromosomes, Plant / genetics; DNA Replication Timing / genetics; DNA Transposable Elements / genetics; Genes, Plant; Genomics; Meristem / cytology; Meristem / genetics; Mitosis / genetics; Models, Genetic; S Phase / genetics; Tandem Repeat Sequences / genetics; Time Factors; Transcription, Genetic; Zea mays / cytology; Zea mays / genetics
TL;DR:
A novel adaptation of the “Repli-seq” assay for use in intact root tips of maize (Zea mays) that includes several different cell lineages and present whole-genome replication timing profiles from cells in early, mid, and late S phase of the mitotic cell cycle is reported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2017 journal article
Repliscan: a tool for classifying replication timing regions
BMC BIOINFORMATICS, 18, 1–14.
author keywords: DNA replication; Repli-seq; Classification
MeSH headings : DNA Replication Timing; Genome; Genome Size; High-Throughput Nucleotide Sequencing / methods; Sequence Analysis, DNA / methods; Software
TL;DR:
To accurately detect and classify regions of replication across the genome, Repliscan is presented, which robustly normalizes, automatically removes outlying and uninformative data points, and classifies Repli-seq signals into discrete combinations of replication signatures.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2016 journal article
A flow cytometric method for estimating S-phase duration in plants
JOURNAL OF EXPERIMENTAL BOTANY, 67(21), 6077–6087.
author keywords: Arabidopsis; barley; EdU; flow cytometry; maize; replication timing; rice; S-phase duration; wheat
MeSH headings : Arabidopsis / cytology; Arabidopsis / growth & development; DNA, Plant / metabolism; Deoxyuridine / analogs & derivatives; Deoxyuridine / metabolism; Flow Cytometry / methods; G1 Phase / physiology; G2 Phase / physiology; Hordeum / cytology; Hordeum / growth & development; Meristem / cytology; Meristem / growth & development; Oryza / cytology; Oryza / growth & development; S Phase / physiology; Triticum / cytology; Triticum / growth & development; Zea mays / cytology; Zea mays / growth & development
TL;DR:
This work estimated S-phase duration for several plant species by following EdU-labeled nuclei from G1 to G2 using bivariate flow cytometry, finding that S- phase duration is relatively consistent over a range of genome sizes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2016 chapter
Isolation of Plant Nuclei at Defined Cell Cycle Stages Using EdU Labeling and Flow Cytometry
In Methods in Molecular Biology (pp. 69–86).
author keywords: 5-Ethynyl-2 '-deoxyuridine; Flow cytometry; Nuclei sorting; Arabidopsis; Maize; Percoll gradient; Cell cycle; DNA replication; Chromatin
MeSH headings : Arabidopsis / cytology; Arabidopsis / genetics; Cell Cycle; Cell Fractionation / methods; Cell Nucleus / genetics; Click Chemistry / methods; DNA Replication; DNA, Plant / analysis; DNA, Plant / genetics; Deoxyuridine / analogs & derivatives; Deoxyuridine / analysis; Flow Cytometry / methods; Fluorescent Dyes / analysis; Zea mays / cytology; Zea mays / genetics
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Crossref, NC State University Libraries, ORCID
Added: February 14, 2019
2016 journal article
Two Novel DNAs That Enhance Symptoms and Overcome CMD2 Resistance to Cassava Mosaic Disease
JOURNAL OF VIROLOGY, 90(8), 4160–4173.
TL;DR:
The discovery of two novel DNA sequences, designated SEGs-1 and SEGS-2 (for sequences enhancing geminivirus symptoms), that enhance symptoms and break resistance to CMD have major implications for the long-term durability of CMD2 resistance and underscore the need for alternative sources of resistance in cassava.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2015 journal article
Defining multiple, distinct, and shared spatiotemporal patterns of DNA replication and endoreduplication from 3D image analysis of developing maize (Zea mays L.) root tip nuclei
PLANT MOLECULAR BIOLOGY, 89(4-5), 339–351.
author keywords: Cell cycle; DNA synthesis; Grass
MeSH headings : Cell Nucleus / genetics; Cell Nucleus / metabolism; DNA Replication / genetics; DNA Replication Timing / genetics; DNA, Plant / biosynthesis; DNA, Plant / genetics; Endoreduplication / genetics; Genes, Plant; Imaging, Three-Dimensional; Meristem / genetics; Meristem / growth & development; Meristem / metabolism; Models, Biological; S Phase / genetics; Zea mays / genetics; Zea mays / growth & development; Zea mays / metabolism
TL;DR:
3D quantitative analysis of DNA replication and endoreduplication in nuclei from pulse-labeled developing maize root tips revealed that maize euchromatin exists as an intermingled mixture of two components distinguished by their condensation state and replication timing.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2014 review
A maize root tip system to study DNA replication programmes in somatic and endocycling nuclei during plant development
[Review of ]. JOURNAL OF EXPERIMENTAL BOTANY, 65(10), 2747–2756.
author keywords: DNA replication; EdU; endocycle; flow cytometry; high-resolution microscopy; maize; mitotic cell cycle; root development; S phase
MeSH headings : Base Sequence; Cell Nucleus / genetics; DNA Replication; DNA, Plant / biosynthesis; In Situ Hybridization, Fluorescence; Oligonucleotide Probes; Plant Roots / growth & development; Zea mays / genetics; Zea mays / growth & development
TL;DR:
A promising new system that can be used to visualize DNA replication in isolated maize root tip nuclei after in planta pulse labelling with the thymidine analogue, 5-ethynyl-2'-deoxyuridine (EdU).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2014 journal article
In Vivo Mapping of Arabidopsis Scaffold/Matrix Attachment Regions Reveals Link to Nucleosome-Disfavoring Poly(dA:dT) Tracts
PLANT CELL, 26(1), 102–120.
MeSH headings : Arabidopsis / genetics; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Arabidopsis Proteins / physiology; Chromatin / metabolism; Gene Expression Regulation, Plant; Matrix Attachment Regions; Nucleosomes / metabolism; Nucleotide Motifs; Poly dA-dT / metabolism; Transcription Factors / genetics; Transcription Factors / metabolism; Transcription Factors / physiology
TL;DR:
This work uses tiling microarrays to map S/MARs on Arabidopsis chromosome 4, found to be spaced more closely than in the large plant and animal genomes studied to date and preferentially enriched in poly(dA:dT) tracts, sequences that resist nucleosome formation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2014 journal article
SnRK1 Phosphorylation of AL2 Delays Cabbage Leaf Curl Virus Infection in Arabidopsis
JOURNAL OF VIROLOGY, 88(18), 10598–10612.
MeSH headings : Amino Acid Sequence; Arabidopsis / enzymology; Arabidopsis / genetics; Arabidopsis / virology; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Begomovirus / chemistry; Begomovirus / classification; Begomovirus / genetics; Begomovirus / metabolism; Host-Pathogen Interactions; Molecular Sequence Data; Phylogeny; Plant Diseases / virology; Protein Binding; Protein Kinases / genetics; Protein Kinases / metabolism; Transcription Factors / genetics; Transcription Factors / metabolism; Viral Proteins / chemistry; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR:
It is shown that the host protein kinase SnRK1, a central regulator of energy balance and nutrient metabolism in plants, phosphorylates serine-109 in AL2 proteins of three subgroups of New World begomoviruses, resulting in a delay in viral DNA accumulation and symptom appearance.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2013 review
Geminiviruses: masters at redirecting and reprogramming plant processes
[Review of ]. NATURE REVIEWS MICROBIOLOGY, 11(11), 777–788.
MeSH headings : Geminiviridae / physiology; Gene Expression Regulation, Viral / physiology; Genome, Viral; Host-Pathogen Interactions; Plant Diseases / virology; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR:
This Review describes the current knowledge of how geminiviruses interact with their plant hosts and the functional consequences of these interactions.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science; OpenAlex)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2013 journal article
Peptide Aptamers That Bind to Geminivirus Replication Proteins Confer a Resistance Phenotype to Tomato Yellow Leaf Curl Virus and Tomato Mottle Virus Infection in Tomato
JOURNAL OF VIROLOGY, 87(17), 9691–9706.
MeSH headings : Amino Acid Sequence; Aptamers, Peptide / genetics; Aptamers, Peptide / metabolism; Begomovirus / genetics; Begomovirus / pathogenicity; Begomovirus / physiology; DNA, Viral / genetics; DNA, Viral / metabolism; Geminiviridae / genetics; Geminiviridae / pathogenicity; Geminiviridae / physiology; Solanum lycopersicum / virology; Molecular Sequence Data; Phenotype; Plant Diseases / genetics; Plant Diseases / prevention & control; Plant Diseases / virology; Plants, Genetically Modified; Protein Binding; Two-Hybrid System Techniques; Viral Proteins / genetics; Viral Proteins / physiology; Virus Replication
TL;DR:
The efficacy of using Rep-binding peptide aptamers to develop crops that are resistant to diverse geminiviruses is established.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
Functional Analysis of a Novel Motif Conserved across Geminivirus Rep Proteins
JOURNAL OF VIROLOGY, 85(3), 1182–1192.
MeSH headings : Amino Acid Motifs; Amino Acid Sequence; Conserved Sequence; DNA Helicases / genetics; DNA Helicases / metabolism; DNA, Viral / metabolism; Geminiviridae / enzymology; Geminiviridae / genetics; Geminiviridae / physiology; Molecular Sequence Data; Mutation, Missense; Plant Diseases / virology; Protein Binding; Sequence Alignment; Tobacco / virology; Trans-Activators / genetics; Trans-Activators / metabolism; Viral Proteins / genetics; Viral Proteins / metabolism; Virulence; Virus Replication
TL;DR:
It is established that the GRS is a conserved, essential motif characteristic of an ancient lineage of rolling-circle initiators and support the idea that geminiviruses may have evolved from plasmids associated with phytoplasma or algae.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
Interaction between Geminivirus Replication Protein and the SUMO-Conjugating Enzyme Is Required for Viral Infection
JOURNAL OF VIROLOGY, 85(19), 9789–9800.
MeSH headings : Amino Acid Substitution; Binding Sites; DNA Mutational Analysis; Geminiviridae / pathogenicity; Host-Pathogen Interactions; Mutagenesis, Site-Directed; Mutant Proteins / genetics; Mutant Proteins / metabolism; Protein Binding; Protein Interaction Mapping; Sequence Deletion; Tobacco / virology; Ubiquitin-Conjugating Enzymes / metabolism; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR:
The results established the importance of AL1-SCE1 interactions during geminivirus infection of plants and suggested that AL1 alters the sumoylation of selected host factors to create an environment suitable for viral infection.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2011 journal article
Tomato SlSnRK1 Protein Interacts with and Phosphorylates beta C1, a Pathogenesis Protein Encoded by a Geminivirus beta-Satellite
PLANT PHYSIOLOGY, 157(3), 1394–1406.
MeSH headings : DNA, Viral / metabolism; Geminiviridae / metabolism; Geminiviridae / pathogenicity; Gene Expression Regulation, Plant; Solanum lycopersicum / enzymology; Solanum lycopersicum / genetics; Solanum lycopersicum / virology; Mutation / genetics; Phosphorylation; Phosphoserine / metabolism; Phosphothreonine / metabolism; Plant Diseases / virology; Plant Proteins / chemistry; Plant Proteins / genetics; Plant Proteins / metabolism; Protein Binding; Protein Interaction Domains and Motifs; Satellite Viruses / metabolism; Satellite Viruses / pathogenicity; Subcellular Fractions / metabolism; Viral Proteins / chemistry; Viral Proteins / metabolism
TL;DR:
Results suggested that the SlSnRK1 protein attenuates geminivirus infection by interacting with and phosphorylating the βC1 protein.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2010 journal article
Arabidopsis thaliana Chromosome 4 Replicates in Two Phases That Correlate with Chromatin State
PLoS Genetics, 6(6), e1000982.
Ed(s): G. Copenhaver
MeSH headings : Arabidopsis / cytology; Arabidopsis / genetics; Chromatin / genetics; Chromosomes, Plant; DNA Replication; Epigenesis, Genetic; Flow Cytometry; Oligonucleotide Array Sequence Analysis; Replicon; S Phase
TL;DR:
The similarity of the replication profiles for early and mid S phase cells indicates that replication origin activation in euchromatin is stochastic, and distinct patterns of association of H3K56ac with gene expression and early replication provide evidence that H3k56ac may be associated with initiation zones and replication origins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
15. Life on Land
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: August 6, 2018
2010 journal article
The E2FD/DEL2 factor is a component of a regulatory network controlling cell proliferation and development in Arabidopsis
Plant Molecular Biology, 72(4-5), 381–395.
author keywords: Arabidopsis; Auxin; E2F transcription factors; Plant cell cycle; Plant development
MeSH headings : Arabidopsis / genetics; Arabidopsis / growth & development; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Base Sequence; Cell Cycle / genetics; Cell Proliferation; DNA, Plant / genetics; E2F Transcription Factors / genetics; E2F Transcription Factors / metabolism; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Gene Regulatory Networks; Genes, Plant; Indoleacetic Acids / metabolism; Molecular Sequence Data; Mutation; Plants, Genetically Modified; Protein Processing, Post-Translational
TL;DR:
The analysis of mutant and transgenic plants characterized by altered expression of E2FD/DEL2 has revealed that this atypical E2F can affect plant growth by promoting cell proliferation and repressing cell elongation and these results suggest that E2 FD/D EL2 is part of a regulatory network that controls the balance betweencell proliferation and development in Arabidopsis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2009 journal article
Arabidopsis Protein Kinases GRIK1 and GRIK2 Specifically Activate SnRK1 by Phosphorylating Its Activation Loop
PLANT PHYSIOLOGY, 150(2), 996–1005.
MeSH headings : Adenosine Monophosphate / pharmacology; Amino Acid Sequence; Arabidopsis / drug effects; Arabidopsis / enzymology; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / metabolism; Benzimidazoles / pharmacology; Cations / pharmacology; Conserved Sequence; Enzyme Activation / drug effects; Molecular Sequence Data; Naphthalimides / pharmacology; Phosphorylation / drug effects; Phosphothreonine / metabolism; Protein Binding / drug effects; Protein Serine-Threonine Kinases / chemistry; Protein Serine-Threonine Kinases / metabolism; Substrate Specificity / drug effects
TL;DR:
The GRIK-SnRK1 cascade may function in a developmentally regulated fashion and coordinate the unique metabolic requirements of rapidly growing cells and geminivirus-infected cells that have been induced to reenter the cell cycle.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2009 journal article
Dynamic Localization of the DNA Replication Proteins MCM5 and MCM7 in Plants
PLANT PHYSIOLOGY, 150(2), 658–669.
MeSH headings : Arabidopsis / cytology; Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / metabolism; Cell Cycle; Cell Cycle Proteins / metabolism; Cell Nucleus / metabolism; Chromatin / metabolism; DNA Replication; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Minichromosome Maintenance Complex Component 7; Multiprotein Complexes / genetics; Multiprotein Complexes / metabolism; Origin Recognition Complex / metabolism; Plant Roots / cytology; Plant Roots / metabolism; Protein Binding; Protein Subunits / genetics; Protein Subunits / metabolism; Protein Transport; Tobacco / cytology; Tobacco / metabolism
TL;DR:
It is demonstrated that subunits of the MCM2-7 complex are coordinately expressed during Arabidopsis (Arabidopsis thaliana) development and are abundant in proliferating and endocycling tissues, indicative of a role in DNA replication.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2009 chapter
Isolation of Peptide Aptamers to Target Protein Function
In Methods in Molecular Biology (pp. 333–360).
MeSH headings : Amino Acid Sequence; Aptamers, Peptide / chemistry; Aptamers, Peptide / genetics; Aptamers, Peptide / metabolism; Peptide Library; Protein Binding; Protein Interaction Domains and Motifs; Proteins / chemistry; Proteins / genetics; Proteins / metabolism; SELEX Aptamer Technique / methods; Two-Hybrid System Techniques
TL;DR:
High-throughput protocols that facilitate the selection and characterization of peptide aptamers from yeast dihybrid libraries are described, including the preparation and evaluation of the bait fusion and the peptideaptamer screen.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
2008 review
Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection
[Review of ]. Plant Physiology, 148(1), 436–454.
Source: NC State University Libraries
Added: August 6, 2018
2007 journal article
Geminivirus-induced gene silencing of the tobacco retinoblastoma-related gene results in cell death and altered development
PLANT MOLECULAR BIOLOGY, 65(1-2), 163–175.
author keywords: DNA VIGS; retinoblastoma-related protein; cell death; geminivirus; hyperplasia; cell differentiation
MeSH headings : Cell Death; DNA, Viral / genetics; Geminiviridae / genetics; Gene Expression Regulation, Plant / genetics; Gene Silencing; Genetic Vectors / genetics; Plant Leaves / genetics; Plant Leaves / growth & development; Plant Leaves / metabolism; Retinoblastoma / genetics; Retinoblastoma / metabolism; Tobacco / cytology; Tobacco / genetics; Tobacco / growth & development
TL;DR:
The results suggest that either inappropriate activation of the cell cycle is lethal in plants or that RBR has other functions, unrelated to the cell Cycle, and demonstrate that continual transcription of RBR is necessary for cell survival.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2007 review
Genome-wide analysis of the core DNA replication machinery in the higher plants Arabidopsis and rice(1[W][OA])
[Review of ]. Plant Physiology, 144(4), 1697–1714.
Source: NC State University Libraries
Added: August 6, 2018
2007 journal article
High-frequency reversion of geminivirus replication protein mutants during infection
JOURNAL OF VIROLOGY, 81(20), 11005–11015.
MeSH headings : Arabidopsis / virology; Geminiviridae / genetics; Geminiviridae / physiology; Mosaic Viruses; Mutation, Missense; Plant Diseases / virology; Plant Viruses / genetics; Plant Viruses / physiology; Viral Proteins / genetics; Viral Proteins / metabolism; Viral Proteins / physiology; Virus Replication / genetics
TL;DR:
There is strong selection for reversion of the TGMV L148V and CaLCuV L145A mutations but that the nature of the selected revertants is influenced by both the viral background and host components.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2006 journal article
Geminivirus infection up-regulates the expression of two Arabidopsis protein kinases related to yeast SNF1-and mammalian AMPK-activating kinases
PLANT PHYSIOLOGY, 142(4), 1642–1655.
MeSH headings : AMP-Activated Protein Kinase Kinases; Amino Acid Sequence; Animals; Arabidopsis / enzymology; Arabidopsis / genetics; Arabidopsis / virology; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Flowers / genetics; Flowers / metabolism; Flowers / virology; Fungal Proteins / chemistry; Geminiviridae / physiology; Gene Expression Regulation, Plant; Genetic Complementation Test; Molecular Sequence Data; Phylogeny; Plant Diseases / virology; Plant Leaves / genetics; Plant Leaves / metabolism; Plant Leaves / virology; Plant Shoots / genetics; Plant Shoots / metabolism; Plant Shoots / virology; Protein Kinases / chemistry; Protein Serine-Threonine Kinases / chemistry; Protein Serine-Threonine Kinases / genetics; Protein Serine-Threonine Kinases / metabolism; RNA, Messenger / metabolism; Sequence Alignment; Two-Hybrid System Techniques; Up-Regulation; Viral Proteins / metabolism
TL;DR:
Both full-length GRIK1 and GRIk2 interact with AL1 in yeast two-hybrid studies, suggesting that the Arabidopsis kinases mediate one or more processes during early plant development and geminivirus infection by activating SNF1-related kinases.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2006 journal article
Peptide aptamers that bind to a geminivirus replication protein interfere with viral replication in plant cells
JOURNAL OF VIROLOGY, 80(12), 5841–5853.
MeSH headings : Aptamers, Peptide / pharmacology; DNA, Viral / analysis; Geminiviridae / chemistry; Geminiviridae / drug effects; Geminiviridae / physiology; Peptide Library; Plant Cells; Plant Viruses / drug effects; Plants / virology; Plants, Genetically Modified; Thioredoxins / genetics; Thioredoxins / metabolism; Transfection; Two-Hybrid System Techniques; Viral Proteins / antagonists & inhibitors; Viral Proteins / genetics; Viral Proteins / metabolism; Viral Proteins / physiology; Virus Replication / drug effects
TL;DR:
The peptide aptamers characterized in these studies represent new tools for studying AL1 function and can serve as the basis for the development of crops with broad-based resistance to single-stranded DNA viruses.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2005 journal article
A trichloroacetic acid-acetone method greatly reduces infrared autofluorescence of protein extracts from plant tissue
PLANT MOLECULAR BIOLOGY REPORTER, 23(4), 405–409.
author keywords: Arabidopsis; autofluorescence; immunoblot; infrared; Odyssey; plant
TL;DR:
A trichloroacetic acid-acetone method is reported that effectively eliminates autofluorescence while retaining the immunogenicity of a target protein.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2005 journal article
Geminivirus C3 protein: Replication enhancement and protein interactions
JOURNAL OF VIROLOGY, 79(15), 9885–9895.
MeSH headings : Amino Acid Sequence; Consensus Sequence; Geminiviridae / metabolism; Geminiviridae / physiology; Molecular Sequence Data; Plant Diseases / virology; Plant Proteins / metabolism; Proliferating Cell Nuclear Antigen / metabolism; Protein Binding; Tobacco; Viral Proteins / genetics; Viral Proteins / metabolism; Virus Replication
TL;DR:
Investigating the impact of mutations to amino acids that are conserved across the C3 protein family on replication enhancement and protein interactions in dicot-infecting geminivirus replication established the importance of C3-C3, C2-C1, and C4-PCNA interactions in geminIVirus replication.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2004 journal article
A novel motif in geminivirus replication proteins interacts with the plant retinoblastoma-related protein
JOURNAL OF VIROLOGY, 78(9), 4817–4826.
MeSH headings : Amino Acid Motifs; Amino Acid Sequence; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Geminiviridae / metabolism; Geminiviridae / pathogenicity; Solanum lycopersicum / virology; Molecular Sequence Data; Mutation; Plant Diseases / virology; Plant Leaves / virology; Plant Proteins / metabolism; Retinoblastoma Protein / metabolism; Tobacco / virology; Two-Hybrid System Techniques; Viral Proteins / chemistry; Viral Proteins / genetics; Viral Proteins / metabolism; Virus Replication
TL;DR:
Results suggest that helix 4 and the conserved leucine residue are part of a pRBR-binding interface in begomovirus replication proteins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2004 journal article
Establishment of rapidly proliferating rice cell suspension culture and its characterization by fluorescence-activated cell sorting analysis
PLANT MOLECULAR BIOLOGY REPORTER, 22(3), 259–267.
author keywords: cell suspension culture; FACS; nuclei isolation; Oryza sativa L.; pectinase
TL;DR:
The studies described here demonstrate that NB2P culture can be a powerful tool for studying many important plant processes, including DNA replication and cell cycle-related pathways.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2004 patent
Geminivirus resistant transgenic plants
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
2004 patent
Geminivirus resistant transgenic plants expressing a mutant geminivirus AL3/C3 coding sequence
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
2004 journal article
Interaction between a geminivirus replication protein and the plant sumoylation system
JOURNAL OF VIROLOGY, 78(6), 2758–2769.
MeSH headings : Amino Acid Sequence; Base Sequence; Geminiviridae / metabolism; Geminiviridae / pathogenicity; Geminiviridae / physiology; Molecular Sequence Data; Plants, Genetically Modified; Sequence Analysis, DNA; Tobacco / genetics; Tobacco / virology; Two-Hybrid System Techniques; Ubiquitin-Conjugating Enzymes / genetics; Ubiquitin-Conjugating Enzymes / metabolism; Viral Proteins / genetics; Viral Proteins / metabolism; Virus Replication
TL;DR:
Effects on geminivirus replication were observed in transgenic plants with altered levels of SUMO, the substrate for UBC9, and the host cell sumoylation enzyme, NbSCE1 (N. benthamiana SUMO-conjugating enzyme, homolog to Saccharomyces cerevisiae U BC9), was found to interact specifically with RepAC1.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2004 review
Reprogramming plant gene expression: a prerequisite to geminivirus DNA replication
[Review of ]. MOLECULAR PLANT PATHOLOGY, 5(2), 149–156.
TL;DR:
Recent experiments showing that geminiviruses also modulate components of the retinoblastoma/E2F transcription regulatory network to induce quiescent plant cells to re-enter the cell cycle and regain the capacity to support high levels of DNA replication are discussed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2002 journal article
A geminivirus replication protein interacts with a protein kinase and a motor protein that display different expression patterns during plant development and infection
PLANT CELL, 14(8), 1817–1832.
MeSH headings : Animals; Arabidopsis / genetics; Arabidopsis / growth & development; Arabidopsis / virology; Arabidopsis Proteins / genetics; Arabidopsis Proteins / isolation & purification; Arabidopsis Proteins / metabolism; Biological Transport / physiology; DNA, Viral / genetics; Geminiviridae / genetics; Geminiviridae / growth & development; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Histones / metabolism; In Situ Hybridization; Kinesins / genetics; Kinesins / metabolism; Microtubule Proteins / metabolism; Mitosis / genetics; Mitosis / physiology; Molecular Sequence Data; Phosphorylation; Protein Binding; Protein Interaction Mapping; Protein Kinases / genetics; Protein Kinases / isolation & purification; Protein Kinases / metabolism; Protein Serine-Threonine Kinases / metabolism; Tobacco / virology; Two-Hybrid System Techniques; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR:
The results establish that AL1 interacts with host proteins involved in plant cell division and development, including a Ser/Thr kinase, a kinesin, and histone H3 as AL1 partners.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2002 journal article
Host DNA replication is induced by geminivirus infection of differentiated plant cells
PLANT CELL, 14(12), 2995–3007.
MeSH headings : Aphidicolin / pharmacology; Bromodeoxyuridine / metabolism; Cell Differentiation / genetics; Cell Differentiation / physiology; DNA Replication / drug effects; DNA Replication / genetics; DNA Replication / physiology; DNA, Viral / drug effects; DNA, Viral / metabolism; Geminiviridae / genetics; Geminiviridae / physiology; Hydroxyurea / pharmacology; Immunohistochemistry; In Situ Hybridization; Microscopy, Fluorescence; Tobacco / chemistry; Tobacco / genetics; Tobacco / virology
TL;DR:
5-bromo-2-deoxyuridine incorporation to examine DNA synthesis directly in infected Nicotiana benthamiana plants to determine if viral reprogramming of host replication controls had an impact on host DNA replication and demonstrate that chromosomal DNA is replicated in TGMV-infected cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2002 journal article
Two E2F elements regulate the proliferating cell nuclear antigen promoter differently during leaf development
PLANT CELL, 14(12), 3225–3236.
MeSH headings : Base Sequence; Binding Sites / genetics; Cell Cycle Proteins; DNA-Binding Proteins; E2F Transcription Factors; E2F1 Transcription Factor; Geminiviridae / genetics; Geminiviridae / physiology; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Molecular Sequence Data; Mutation; Plant Leaves / genetics; Plant Leaves / growth & development; Plant Leaves / virology; Plants, Genetically Modified; Proliferating Cell Nuclear Antigen / genetics; Proliferating Cell Nuclear Antigen / metabolism; Promoter Regions, Genetic / genetics; Protein Binding; Response Elements / genetics; Tobacco / genetics; Tobacco / growth & development; Tobacco / virology; Transcription Factors / genetics; Transcription Factors / metabolism
TL;DR:
Examination of the distal element (E2F1) and how it interacts with the E2F2 site to regulate the PCNA promoter demonstrates that both E2f elements contribute to the repression of thePCNA promoter in mature leaves, whereas the E 2F1 site counters the repression activity of the E1F2 element in young leaves.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2001 journal article
Dual interaction of a geminivirus replication accessory factor with a viral replication protein and a plant cell cycle regulator
VIROLOGY, 279(2), 570–576.
MeSH headings : Cell Cycle; Geminiviridae / physiology; Plant Proteins / metabolism; Protein Binding; Retinoblastoma Protein / metabolism; Viral Proteins / metabolism; Virus Replication
TL;DR:
Experiments reported here established that AL3 also interacts with a plant homolog of the mammalian tumor suppressor protein, retinoblastoma (pRb), and analysis of truncated AL3 proteins indicated that pRb and AL1 bind to similar regions of AL3, whereas AL3 oligomerization is dependent on a different region of the protein.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2001 journal article
Proliferating cell nuclear antigen transcription is repressed through an E2F consensus element and activated by geminivirus infection in mature leaves
PLANT CELL, 13(6), 1437–1452.
MeSH headings : Base Sequence; Binding Sites; Cell Cycle Proteins; Consensus Sequence; DNA, Complementary; DNA, Plant; DNA-Binding Proteins; E2F Transcription Factors; Geminiviridae / genetics; Geminiviridae / physiology; Gene Expression Regulation, Plant; Gene Expression Regulation, Viral; Molecular Sequence Data; Mosaic Viruses / genetics; Mosaic Viruses / physiology; Mutation; Plant Leaves / metabolism; Plant Leaves / virology; Plants, Genetically Modified; Plants, Toxic; Proliferating Cell Nuclear Antigen / genetics; Promoter Regions, Genetic; Tobacco / genetics; Tobacco / virology; Transcription Factors / metabolism; Transcription, Genetic
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2001 journal article
Silencing of a meristematic gene using geminivirus-derived vectors
PLANT JOURNAL, 27(4), 357–366.
author keywords: Geminiviruses; homology-dependent gene silencing; meristems; functional genomics; plant DNA viruses; magnesium chelatase; proliferating cell nuclear antigen
MeSH headings : Base Sequence; DNA Primers; DNA, Viral / metabolism; Geminiviridae / genetics; Gene Silencing; Genes, Essential; Genetic Vectors; In Situ Hybridization; Meristem / genetics; Tobacco / genetics; Tobacco / virology
TL;DR:
The results demonstrate that geminivirus-derived vectors can be used to study genes involved in meristem function in intact plants and show that endogenous gene silencing can be separated from viral infection.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2000 journal article
A geminivirus replication protein interacts with the retinoblastoma protein through a novel domain to determine symptoms and tissue specificity of infection in plants
EMBO JOURNAL, 19(13), 3485–3495.
author keywords: AL1; cell cycle; differentiation; plant DNA virus; pRb
MeSH headings : Antigens, Polyomavirus Transforming / metabolism; Base Sequence; DNA Primers; Geminiviridae / isolation & purification; Geminiviridae / metabolism; Geminiviridae / physiology; Plants / virology; Protein Binding; Retinoblastoma Protein / metabolism; Viral Proteins / metabolism; Virus Replication
TL;DR:
The pRBR binding domain of AL1 is mapped between amino acids 101 and 180 and two mutants are identified that are differentially impacted for AL1–pRBR interactions during geminivirus infection of plants.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2000 journal article
Chromosome condensation induced by geminivirus infection of mature plant cells
Journal of Cell Science, 113(7), 1149–1160.
Source: NC State University Libraries
Added: August 6, 2018
2000 journal article
Geminiviruses - Models for plant DNA replication, transcription and cell cycle regulation ([correction to] vol 35, pg 105, 2000)
Critical Reviews in Biochemistry and Molecular Biology, 35(4), U4.
Source: NC State University Libraries
Added: August 6, 2018
2000 journal article
Geminiviruses: Models for plant DNA replication, transcription, and cell cycle regulation
Critical Reviews in Biochemistry and Molecular Biology, 35(2), 105–140.
Source: NC State University Libraries
Added: August 6, 2018
2000 journal article
The multifunctional character of a geminivirus replication protein is reflected by its complex oligomerization properties
JOURNAL OF BIOLOGICAL CHEMISTRY, 275(9), 6114–6122.
MeSH headings : Amino Acid Sequence; Animals; Conserved Sequence; DNA / metabolism; DNA-Binding Proteins / chemistry; Geminiviridae / chemistry; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Protein Conformation; Spodoptera / genetics; Transcription, Genetic; Transfection; Viral Proteins / chemistry; Viral Proteins / genetics; Virus Replication; Yeasts
TL;DR:
Results suggested that different AL1 complexes mediate viral replication and transcriptional regulation and that replication interference involves multiple domains of the AL1 protein.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1999 journal article
Geminiviruses: Models for plant DNA replication, transcription, and cell cycle regulation {review}
Critical Reviews in Plant Sciences, 18(1), 71–106.
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: NC State University Libraries
Added: August 6, 2018
1998 journal article
Conserved sequence and structural motifs contribute to the DNA binding and cleavage activities of a geminivirus replication protein
JOURNAL OF BIOLOGICAL CHEMISTRY, 273(38), 24448–24456.
MeSH headings : Amino Acid Sequence; Animals; Baculoviridae; Cell Line; Conserved Sequence; DNA, Single-Stranded / metabolism; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / metabolism; Geminiviridae / physiology; Genetic Vectors; Glutathione Transferase; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Structure, Secondary; Recombinant Fusion Proteins / chemistry; Recombinant Fusion Proteins / metabolism; Spodoptera; Transfection; Viral Proteins / chemistry; Viral Proteins / genetics; Viral Proteins / metabolism; Virus Replication
TL;DR:
Results established that the conserved motifs in the AL1 N terminus contribute to DNA binding and cleavage with both activities displaying nearly identical amino acid requirements, however, DNA binding was readily distinguished from cleavage and ligation by its dependence on AL1/AL1 interactions.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1998 journal article
Multiple cis elements contribute to geminivirus origin function
VIROLOGY, 242(2), 346–356.
MeSH headings : Base Sequence; DNA Replication / genetics; DNA Replication / physiology; DNA, Circular / genetics; DNA, Circular / physiology; DNA, Viral / genetics; DNA, Viral / physiology; Geminiviridae / genetics; Geminiviridae / physiology; Solanum lycopersicum / virology; Molecular Sequence Data; Nucleic Acid Conformation; Regulatory Sequences, Nucleic Acid; Replication Origin / genetics; Virus Replication / genetics; Virus Replication / physiology
TL;DR:
The results demonstrated that the organization of TGMV plus-strand origin is complex, involving multiple cis elements that are likely to interact with each other during initiation of replication.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1997 journal article
CIS elements that contribute to geminivirus transcriptional regulation and the efficiency of DNA replication
Journal of Virology, 71(9), 6947–6955.
Source: NC State University Libraries
Added: August 6, 2018
1997 journal article
Functional domains of a geminivirus replication protein
Journal of Biological Chemistry, 272(15), 9840–9846.
MeSH headings : Amino Acid Sequence; DNA / metabolism; Electrophoresis, Polyacrylamide Gel; Molecular Sequence Data; Peptide Mapping; Promoter Regions, Genetic; Protein Conformation; Viral Proteins / chemistry; Viral Proteins / genetics; Virus Replication
TL;DR:
To identify the tomato golden mosaic virus AL1 domains that mediate protein oligomerization, DNA binding, and DNA cleavage, a series of truncated AL1 proteins were produced in a baculovirus expression system and assayed for each activity.
(via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018
1997 journal article
RRB1 and RRB2 encode maize retinoblastoma-related proteins that interact with a plant D-type cyclin and geminivirus replication protein
MOLECULAR AND CELLULAR BIOLOGY, 17(9), 5077–5086.
MeSH headings : Amino Acid Sequence; Antigens, Viral, Tumor / metabolism; Binding Sites; Cell Cycle Proteins; Cyclins / metabolism; DNA Helicases / metabolism; DNA-Binding Proteins; Genes, Plant; Humans; Molecular Sequence Data; Nuclear Proteins / genetics; Nuclear Proteins / metabolism; Plant Proteins / chemistry; Plant Proteins / genetics; Plant Proteins / metabolism; Protein Binding; Retinoblastoma Protein / chemistry; Trans-Activators / metabolism; Zea mays / genetics
TL;DR:
The cloning of cDNAs from two Zea mays genes, RRB1 and RRB2, that encode RB-related proteins are reported, suggesting that G1 regulation in plant cells is controlled by a mechanism which is much more similar to that found in mammalian cells than that in yeast.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1997 journal article
Two domains of the AL1 protein mediate geminivirus origin recognition
VIROLOGY, 239(1), 186–197.
MeSH headings : Amino Acid Sequence; Base Sequence; Binding Sites / genetics; Geminiviridae / physiology; Gene Expression Regulation, Viral; Solanum lycopersicum / virology; Molecular Sequence Data; Transcription, Genetic; Viral Proteins / genetics; Virus Replication
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
1996 journal article
A DNA structure is required for geminivirus replication origin function.
Journal of Virology, 70(1), 148–158.
MeSH headings : Base Sequence; DNA Replication; DNA, Circular / chemistry; DNA, Circular / genetics; DNA, Single-Stranded / chemistry; DNA, Single-Stranded / genetics; DNA, Viral / biosynthesis; DNA, Viral / chemistry; DNA, Viral / genetics; DNA, Viral / physiology; Endonucleases / genetics; Endonucleases / metabolism; Geminiviridae / genetics; Geminiviridae / physiology; Solanum lycopersicum / virology; Molecular Sequence Data; Nucleic Acid Conformation; Plant Leaves / virology; Replication Origin / physiology; Structure-Activity Relationship; Viral Proteins / genetics; Viral Proteins / metabolism; Virus Replication / physiology
TL;DR:
In vitro cleavage analysis of two TGMV B mutants with different replication phenotypes indicated that there is a correlation between the two assays for origin activity, suggesting that the in vivo replication results may reflect structural and sequence requirements for DNA cleavage during initiation of rolling-circle replication.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
1996 chapter
Geminivirus Replication
In G. Stacey, B. Mullin, & P. M. Gresshoff (Eds.), Biology of Plant-Microbe Interactions (pp. 287–292). St. Paul, MN: International Society of Molecular Plant-Microbe Interactions.
Ed(s): G. Stacey, B. Mullin & P. Gresshoff
Source: NC State University Libraries
Added: October 4, 2020
1996 journal article
Interactions between geminivirus replication proteins.
Journal of Virology, 70(10), 6790–6795.
MeSH headings : Geminiviridae / physiology; Viral Proteins / metabolism; Virus Replication
TL;DR:
Using recombinant proteins produced in a baculovirus expression system, it is shown that AL1 copurifies with a protein fusion of glutathione S-transferase and AL1, independent of the GST domain, and authentic AL3 cofractionates with a GST-AL3 fusion protein.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
1995 journal article
A geminivirus induces expression of a host DNA synthesis protein in terminally differentiated plant cells.
The Plant Cell, 7(6), 705–719.
MeSH headings : Base Sequence; Cell Differentiation; Geminiviridae / physiology; Gene Expression Regulation, Viral; Humans; Molecular Sequence Data; Oligodeoxyribonucleotides; Plants, Genetically Modified; Plants, Toxic; Proliferating Cell Nuclear Antigen / genetics; Tobacco / cytology; Tobacco / genetics; Tobacco / virology; Virus Replication
TL;DR:
It is shown that the replication proteins of the geminivirus tomato golden mosaic virus (TGMV) are located in nuclei of terminally differentiated cells that have left the cell cycle, and that TGMV infection resulted in a significant accumulation of the host DNA synthesis protein proliferating cell nuclear antigen (PCNA).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
1994 journal article
A DNA sequence required for geminivirus replication also mediates transcriptional regulation.
The Plant Cell, 6(8), 1157–1170.
MeSH headings : Amino Acid Sequence; Base Sequence; Cell Line; DNA, Viral / biosynthesis; DNA, Viral / genetics; DNA, Viral / metabolism; Geminiviridae / genetics; Geminiviridae / growth & development; Gene Expression Regulation, Viral; Solanum lycopersicum / virology; Molecular Sequence Data; Mutagenesis, Site-Directed; Plants, Toxic; Promoter Regions, Genetic / genetics; Protein Binding; Tobacco / cytology; Transcription, Genetic; Transfection; Viral Proteins / biosynthesis; Viral Proteins / genetics; Viral Proteins / metabolism; Virus Replication / genetics
TL;DR:
Analysis of AL1 binding site mutants in transient expression assays demonstrated that the same site, which is located between the transcription start site and TATA box in the AL1 promoter, also mediates transcriptional repression.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
1994 journal article
Geminivirus replication origins have a modular organization.
The Plant Cell, 6(3), 405–416.
MeSH headings : Base Sequence; DNA Replication; DNA, Viral / genetics; DNA, Viral / metabolism; Geminiviridae / genetics; Geminiviridae / growth & development; Molecular Sequence Data; Nucleic Acid Conformation; Protein Binding; Sequence Homology, Nucleic Acid; Viral Proteins / metabolism; Virus Replication / genetics
TL;DR:
The results suggested that geminivirus replication origins are composed of at least three functional modules: a putative stem-loop structure that is required for replication but does not contribute to virus-specific recognition of the origin, a specific high-affinity binding site for the AL1 protein, and at least one additional element that contributes to specific origin recognition by viral trans-acting factors.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
1994 journal article
Interaction between a geminivirus replication protein and origin DNA is essential for viral replication
Journal of Biological Chemistry, 269, 8459–8465.
Source: NC State University Libraries
Added: September 24, 2020
1994 journal article
Molecular Characterization of the AL3 Protein Encoded by a Bipartite Geminivirus
Virology, 202(2), 1070–1075.
MeSH headings : Cloning, Molecular; Geminiviridae / genetics; Genes, Viral; Immunologic Techniques; Protein Biosynthesis; RNA, Messenger / genetics; RNA, Viral / genetics; Viral Proteins / genetics; Viral Proteins / metabolism; Viral Structural Proteins / genetics
TL;DR:
Analysis of protein extracts from healthy plants and plants infected with TGMV by SDS-PAGE and immunoblotting showed that a protein corresponding to the predicted AL3 gene product is produced only in infected plants.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018
1992 journal article
A geminivirus replication protein is a sequence-specific DNA binding protein.
The Plant Cell, 4(5), 597–608.
MeSH headings : Base Sequence; Binding Sites; Binding, Competitive; DNA, Viral / metabolism; DNA-Binding Proteins / metabolism; Electrophoresis, Polyacrylamide Gel; Molecular Sequence Data; Mosaic Viruses / metabolism; Mosaic Viruses / physiology; Mosaic Viruses / ultrastructure; Plants, Genetically Modified; Viral Proteins / metabolism; Virus Replication
TL;DR:
It is demonstrated that a geminivirus replication protein is a sequence-specific DNA binding protein, and the studies have important implications for the role of this protein in virus replication.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
1992 chapter
Transgenic plants expressing viral genes
In T. M. A. Wilson & J. W. Davies (Eds.), Genetic Engineering with Plant Viruses (pp. 251–295). CRC Press, Inc.
Ed(s): T. Wilson & J. Davies
Source: NC State University Libraries
Added: September 27, 2020
1990 journal article
Expression of functional replication protein from tomato golden mosaic virus in transgenic tobacco plants.
Proceedings of the National Academy of Sciences, 87(4), 1446–1450.
TL;DR:
It is established that the transgenic plants express functional AL1 protein and show that this viral protein is not only required, but sufficient, for single- and double-stranded replication of TGMV DNA in the presence of host proteins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
1989 journal article
Functional expression of the leftward open reading frames of the A component of tomato golden mosaic virus in transgenic tobacco plants.
The Plant Cell, 1(11), 1057–1067.
MeSH headings : Base Sequence; Cloning, Molecular; DNA, Viral; Genes, Viral; Genetic Complementation Test; Molecular Sequence Data; Mosaic Viruses / genetics; Mosaic Viruses / metabolism; Mutation; Open Reading Frames; Organ Specificity / genetics; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified; Plants, Toxic; RNA, Viral / metabolism; Tobacco / genetics; Tobacco / metabolism; Tobacco / microbiology; Transcription, Genetic
TL;DR:
Evidence that chromosomal position can affect the cell- and tissue-specific transcription of the 35S promoter in transgenic plants is provided, and comparison of the complementing plants and wild-type infected plants is compared to provide insight into the TGMV infection process and the use of the CaMV35S promoter for gene expression intransgenic plants.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
1989 journal article
Transcriptional interaction between the promoters of the maize chloroplast genes which encode the β subunit of ATP synthase and the large subunit of ribulose 1,5-bisphosphate carboxylase
Molecular and General Genetics MGG, 215(2), 217–224.
MeSH headings : Adenosine Triphosphatases / genetics; Chloroplasts / enzymology; Genes; Macromolecular Substances; Plants / enzymology; Plants / genetics; Plasmids; Promoter Regions, Genetic; Restriction Mapping; Ribulose-Bisphosphate Carboxylase / genetics; Transcription, Genetic; Zea mays / enzymology; Zea mays / genetics
TL;DR:
It is established that the atpB and rbcL promoters interact in vitro in a cis and spacing dependent manner and may have physiological relevance in vivo.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
1988 journal article
Characterization of a chloroplast sequence-specific DNA binding factor
Journal of Biological Chemistry, 263, 8288–8293.
Source: NC State University Libraries
Added: October 4, 2020
1988 journal article
Transcription of the wheat chloroplast gene that encodes the 32 kd polypeptide
Plant Molecular Biology, 10(4), 303–310.
TL;DR:
The wheat chloroplast gene (psbA) that encodes the 32 kd polypeptide of Photosystem II is mapped and cloned and the 5′ terminus of the wheat psbA RNA is located to a position 83 nt upstream of its coding region.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
1988 journal article
Transient expression of heterologous RNAs using tomato golden mosaic virus
Nucleic Acids Research, 16(22), 10511–10528.
MeSH headings : DNA Replication; Genes, Viral; Kinetics; Mosaic Viruses / genetics; Plants / genetics; Plants, Toxic; RNA, Viral / genetics; RNA, Viral / isolation & purification; Species Specificity; Tobacco / genetics; Transcription, Genetic; Virus Replication
TL;DR:
The geminivirus transient expression system allows rapid analysis of RNAs transcribed from foreign genes and can serve as a preliminary screen in the construction of transgenic plants.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
1987 journal article
Chloroplast promoters
Trends in Biochemical Sciences, 12, 67–70.
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
1986 chapter
In vitro transcription of chloroplast protein genes
In Methods in Enzymology (pp. 232–253).
MeSH headings : Centrifugation; Chloroplasts / metabolism; DNA / genetics; DNA / metabolism; DNA Restriction Enzymes / metabolism; DNA-Directed RNA Polymerases / metabolism; Endonucleases / metabolism; Fabaceae; Nucleic Acid Hybridization; Plant Extracts / metabolism; Plant Proteins / genetics; Plants; Plants, Medicinal; RNA / genetics; Single-Strand Specific DNA and RNA Endonucleases; Temperature; Transcription, Genetic; Zea mays
TL;DR:
This chapter discusses the methods that are useful for the in vitro analysis of plastid gene expression and the use of a homologousPlastid in vitro transcription system, which is dependent on the gene transcribed and the extract employed.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
1985 journal article
In vitro synthesis and processing of a maize chloroplast transcript encoded by the ribulose 1,5-bisphosphate carboxylase large subunit gene.
Molecular and Cellular Biology, 5(10), 2733–2745.
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
1985 chapter
Transcription of chloroplast genes by homo-logous and heterologous RNA polymerases
In K. E. Steinback, S. Bonitz, C. J. Arntzen, & L. Bogorad (Eds.), Molecular biology of the photosynthetic apparatus (pp. 311–318). Cold Spring Harbor Press.
Ed(s): K. Steinback, S. Bonitz, C. Arntzen & L. Bogorad
Source: NC State University Libraries
Added: September 27, 2020
1983 journal article
A novel protein programmed by the mRNA conserved in dry wheat embryos. The principal site of cysteine incorporation during early germination
European Journal of Biochemistry, 135(1), 9–15.
MeSH headings : Alkylation; Amino Acids / analysis; Cell-Free System; Chromatography; Cysteine / metabolism; Electrophoresis, Polyacrylamide Gel; Lectins / analysis; Plant Lectins; Plant Proteins / biosynthesis; Plant Proteins / isolation & purification; RNA, Messenger / metabolism; Seeds / metabolism; Triticum; Wheat Germ Agglutinins
TL;DR:
It was especially interesting to find that when wheat embryo proteins are alkylated by iodo[14C]acetamide, two proteins co-dominate the distribution of radioalkylated products in dodecylsulphate/polyacrylamide gels: Ec and wheat germ agglutinin.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
1982 journal article
Synthesis and turnover of proteins and mRNA in germinating wheat embryos
Canadian Journal of Biochemistry, 60(3), 389–397.
MeSH headings : Amino Acids / metabolism; Cell-Free System; Methionine / metabolism; Plant Proteins / analysis; Plant Proteins / metabolism; RNA, Messenger / metabolism; Seeds; Time Factors; Triticum; Water / metabolism
TL;DR:
The electrophoretic properties and its time course of emergence, after 5 h postimbibition development, suggest that this major product of cell-free synthesis may be an in vitro counterpart to a prominent methionine-labeled protein made only in vivo, by "growth" phase embryos.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
1981 journal article
Structural integrity of RNA and translational integrity of ribosomes in nuclease-treated cell-free protein synthesizing systems prepared from wheat germ and rabbit reticulocytes
Journal of Biological Chemistry, 256, 5802–5809.
Source: NC State University Libraries
Added: September 24, 2020
1975 journal article
Synthesis and amino acid composition of basic proteins in mammalian sperm nuclei
Developmental Biology, 47(2), 349–365.
MeSH headings : Amino Acids; Animals; Arginine / metabolism; Cell Fractionation; Chemical Phenomena; Chemistry; Guinea Pigs; Humans; Male; Mice; Nucleoproteins / biosynthesis; Nucleoproteins / isolation & purification; Rabbits; Species Specificity; Spermatozoa / metabolism; Spermatozoa / ultrastructure
TL;DR:
Studies on the incorporation of [3H]arginine into mouse SCP demonstrated that these basic proteins are synthesized during the terminal stages of spermatogenesis and are subsequently conserved.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 13, 2020
Employment
Updated: January 21st, 2020 13:17
2015 - present
North Carolina State University Raleigh, NC, US
William Neal Reynolds Distinguished Professor
Plant and Microbial Biology
2015 - present
North Carolina State University Raleigh, NC, US
Associate Member
Molecular and Structural Biochemisty
2005 - 2015
North Carolina State University Raleigh, NC, US
William Neal Reynolds Distinguished Professor
Molecular and Structural Biochemistry
2000 - 2015
North Carolina State University Raleigh, NC, US
Professor
Molecular and Structural Biochemistry
1993 - 2012
North Carolina State University Raleigh, NC, US
Associate Member
Genetics
1995 - 2000
North Carolina State University Raleigh, NC, US
Associate Professor
Biochemistry
1990 - 1995
North Carolina State University Raleigh, NC, US
Assistant Professor
Biochemistry
1987 - 1990
Monsanto Company MO, MO, US
Postdoctoral Fellow
Corporate Research
1986 - 1987
Cornell University Ithaca, New York, US
Research Associate
Plant Pathology
1976 - 1978
Johns Hopkins Medicine Baltimore, MD, US
Research Assistant
Pharmacology and Experimental Therapeutics
1973 - 1976
Harvard Medical School Boston, MA, US
Research Assistant
Human Reproduction and Reproductive Biology
Education
Updated: January 21st, 2020 12:51
1980 - 1986
Rockefeller University New York, NY, US
Ph.D.
1978 - 1981
University of Toronto Toronto, Ontario, CA
M.Sc.
Biochemistry
1968 - 1971
University of California Riverside Riverside, CA, US
BA
Biochemistry
Funding History
Funding history based on the linked ORCID record. Updated: January 21st, 2020 13:56
grant
August 1, 2016 - July 1, 2021
Cassava Mosaic Disease susceptibility and resistance: Translation from Arabidopsis to cassava
Bill and Melinda Gates Foundation
grant
October 1, 2015 - September 1, 2021
U.S. – East Africa Research and Education Partnership: Cassava mosaic disease – A paradigm for the evolution of insect-transmitted plant virus pathosystems
National Science Foundation
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