Laura Lynn Lee

Works (11)

Updated: July 9th, 2023 21:19

2020 article

The biology and biotechnology of the genus Caldicellulosiruptor: recent developments in 'Caldi World'

Lee, L. L., Crosby, J. R., Rubinstein, G. M., Laemthong, T., Bing, R. G., Straub, C. T., … Kelly, R. M. (2020, January). EXTREMOPHILES, Vol. 24, pp. 1–15.

By: L. Lee n, J. Crosby n, G. Rubinstein*, T. Laemthong n, R. Bing n , C. Straub n, M. Adams *, R. Kelly n 

author keywords: Caldicellulosiruptor; Extreme thermophiles; Lignocellulose
MeSH headings : Biomass; Biotechnology; Clostridiales; Glycoside Hydrolases; Hot Springs
TL;DR: Improvements in the amount of carbohydrate released and in the production of bio-based chemicals are required to successfully realize the biotechnological potential of Caldicellulosiruptor organisms. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 27, 2020

2019 journal article

Comparative Biochemical and Structural Analysis of Novel Cellulose Binding Proteins (Tapirins) from Extremely Thermophilic Caldicellulosiruptor Species

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 85(3).

By: L. Lee n, W. Hart  n, V. Lunin, M. Alahuhta*, Y. Bomble *, M. Himmel*, S. Blumer-Schuette  n, M. Adams *, R. Kelly n 

author keywords: Caldicellulosiruptor; cellulose binding protein; lignocellulose; tapirin
MeSH headings : Amino Acid Sequence; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Carrier Proteins / chemistry; Carrier Proteins / genetics; Carrier Proteins / metabolism; Cellulose / chemistry; Cellulose / metabolism; Firmicutes / chemistry; Firmicutes / genetics; Firmicutes / metabolism; Genome, Bacterial; Hot Springs / microbiology; Hot Temperature; Protein Domains
TL;DR: Densitometry analysis of bound protein fractions visualized by SDS-PAGE revealed that Calhy_0908 and Calkr_0826 (from weakly cellulolytic species) associated with the cellulose substrates to a greater extent than Athe_1870, Calkro_0844, and NA10_0869 (from strongly cellulolyic species). (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: February 11, 2019

2019 journal article

Survival and Habitat of Yellow‐Phase American Eels in North Carolina Tidal Creeks

Transactions of the American Fisheries Society, 148(5), 978–990.

By: P. Rudershausen n, L. M. Lee *, S. Lombardo  n, J. Merrell n & J. Buckel n

UN Sustainable Development Goal Categories
14. Life Below Water (Web of Science; OpenAlex)
Sources: Web Of Science, Crossref
Added: October 7, 2019

2018 journal article

Novel multidomain, multifunctional glycoside hydrolases from highly lignocellulolytic Caldicellulosiruptor species

AICHE JOURNAL, 64(12), 4218–4228.

By: J. Conway  n, J. Crosby n, A. Hren n, R. Southerland n, L. Lee n, V. Lunin, P. Alahuhta*, M. Himmel* ...

author keywords: Caldicellulosiruptor; lignocellulose; glycoside hydrolase; cellulase
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: November 26, 2018

2017 journal article

Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(17).

By: J. Zurawski n, P. Khatibi n, H. Akinosho*, C. Straub n, S. Compton n, J. Conway n, L. Lee n, A. Ragauskas * ...

author keywords: Caldicellulosiruptor; switchgrass; lignocellulose deconstruction and conversion; extreme thermophiles; lignocellulose
MeSH headings : Biomass; Fermentation; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / metabolism; Hot Temperature; Lignin / chemistry; Lignin / metabolism; Panicum / chemistry; Panicum / genetics; Panicum / metabolism; Panicum / microbiology; Plants, Genetically Modified / chemistry; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Plants, Genetically Modified / microbiology; Polysaccharides / chemistry; Polysaccharides / metabolism
TL;DR: While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

Genome Stability in Engineered Strains of the Extremely Thermophilic Lignocellulose-Degrading Bacterium Caldicellulosiruptor bescii

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(14).

By: A. Williams-Rhaesa*, F. Poole*, J. Dinsmore*, G. Lipscomb*, G. Rubinstein*, I. Scott*, J. Conway n, L. Lee n ...

author keywords: Southern blotting; genome sequencing; insertion elements; kanamycin resistance
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Genetic Engineering; Genome, Bacterial; Genomic Instability; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / metabolism; Hot Temperature; Lignin / metabolism
TL;DR: It is shown that strains derived from the genetic background containing a random deletion in uracil biosynthesis genes (pyrFA) have a dramatic increase in the number of single nucleotide polymorphisms, insertions/deletions, and ISCbe4 insertion elements in their genomes compared to the wild type. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 review

Physiological, metabolic and biotechnological features of extremely thermophilic microorganisms

[Review of ]. WILEY INTERDISCIPLINARY REVIEWS-SYSTEMS BIOLOGY AND MEDICINE, 9(3).

By: J. Counts n, B. Zeldes n, L. Lee n, C. Straub n, M. Adams * & R. Kelly n 

MeSH headings : Biocatalysis; Carbohydrate Metabolism; Carbon Dioxide / metabolism; Glycolysis; Metabolic Engineering; Metals / chemistry; Metals / metabolism; Sulfolobales / metabolism; Sulfur / metabolism; Thermoanaerobacter / metabolism; Thermococcales / metabolism; Thermus / metabolism
TL;DR: The bacterial genera Caldicellulosiruptor, Thermotoga and Thermus, and the archaea belonging to the orders Thermococcales and Sulfolobales, are among the most studied extreme thermophiles to date. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

Multidomain, Surface Layer-associated Glycoside Hydrolases Contribute to Plant Polysaccharide Degradation by Caldicellulosiruptor Species

JOURNAL OF BIOLOGICAL CHEMISTRY, 291(13), 6732–6747.

By: J. Conway n, W. Pierce n, J. Le n, G. Harper n, J. Wright n, A. Tucker n, J. Zurawski n, L. Lee n, S. Blumer-Schuette n, R. Kelly n 

author keywords: biofuel; cell surface; cell surface enzyme; enzyme; glycoside hydrolase; plant cell wall; Caldicellulosiruptor; S-layer; lignocellulose
MeSH headings : Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Cloning, Molecular; Clostridiales / chemistry; Clostridiales / classification; Clostridiales / enzymology; Escherichia coli / genetics; Escherichia coli / metabolism; Gene Expression; Genome, Bacterial; Glucans / metabolism; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Kinetics; Mutation; Phylogeny; Polysaccharides / metabolism; Protein Binding; Protein Engineering; Protein Structure, Tertiary; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Substrate Specificity; Wood / metabolism; Xylans / metabolism
TL;DR: The results here provide new insights into the architecture and role of SLHdomain GHs and demonstrate that hemicellulose degradation can be enhanced through non-native SLH domain GHs engineered into the genomes of Caldicelluosiruptor species. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Comparative Analysis of Extremely Thermophilic Caldicellulosiruptor Species Reveals Common and Unique Cellular Strategies for Plant Biomass Utilization

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 81(20), 7159–7170.

By: J. Zurawski n, J. Conway n, L. Lee n, H. Simpson  n, J. Izquierdo n, S. Blumer-Schuette n, I. Nookaew *, M. Adams *, R. Kelly n 

MeSH headings : Bacteria / metabolism; Biomass; Cellulase / metabolism; Panicum / enzymology; Panicum / metabolism; Plants / enzymology
TL;DR: Results here show that capacity for plant biomass deconstruction varies across Caldicellulosiruptor species and depends in a complex way on GH genome inventory, substrate composition, and gene regulation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Discrete and Structurally Unique Proteins (Tapirins) Mediate Attachment of Extremely Thermophilic Caldicellulosiruptor Species to Cellulose

JOURNAL OF BIOLOGICAL CHEMISTRY, 290(17), 10645–10656.

By: S. Blumer-Schuette n, M. Alahuhta*, J. Conway n, L. Lee n, J. Zurawski n, R. Giannone *, R. Hettich *, V. Lunin, M. Himmel*, R. Kelly n 

MeSH headings : Adsorption; Bacteria / genetics; Bacteria / metabolism; Bacteria / ultrastructure; Bacterial Adhesion / physiology; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Binding Sites; Cellulose / metabolism; Fimbriae, Bacterial / metabolism; Genes, Bacterial; Models, Molecular; Phylogeny; Plants / microbiology; Protein Structure, Tertiary; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism
TL;DR: Structurally unique, discrete proteins (tāpirins), not directly associated with cellulases, that mediate attachment to cellulose by species in the noncellulosomal, extremely thermophilic bacterial genus Caldicellulosiruptor are identified and define a new class of polysaccharide adhesins. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

journal article

Lignocellulosic biomass deconstruction by the extremely thermophilic genus caldicellulosiruptor

Conway, J. M., Zurawski, J. V., Lee, L. L., Blumer-Schuette, S. E., & Kelly, R. M. Thermophilic Microorganisms, 91–119.

By: J. Conway, J. Zurawski, L. Lee, S. Blumer-Schuette & R. Kelly 

Source: NC State University Libraries
Added: August 6, 2018

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