@article{lorenz-cortes_vinueza-benitez_2024, title={The Role of Textile Dyes in the Immune System Dysregulation}, volume={212}, ISSN={["1550-6606"]}, DOI={10.4049/jimmunol.212.supp.0365.4545}, abstractNote={Abstract There has been an increase in the incidence of atopic diseases and cancer in the last decades. There is an urgent need to identify the environmental triggers that fuel these conditions. In recent years, the use of synthetic textile dyes has increased. The effects of these chemicals or their metabolites on our skin and our immune system have not been well studied. There have been reports on the effects of irritants to the skin on their ability to break the dermal barrier to start a chain of immune reactions that dysregulate the immune system. The purpose of this study was to investigate the effects of the most common textile dyes in the viability, IFNg production and mitochondrial function of epidermal skin, and intestinal epithelial cells using swine as an animal model. Cultures of MPEK-BL6 and IPEC cells were cultured with disperse textile dyes Red 11, Orange 37, Blue 1, Blue 124, and Brown 1, at low and high concentrations for 3-6 hours and 1-3 days. Viability was measured with LUNA cell counter and by flow cytometry. IFNg production by flow cytometry and ELISPOT and Oxygen consumption rate (OCR) by Seahorse Agilent Technology kit. Disperse Blue 124 and Brown 1 induced IFNg production, and impaired cell viability and mitochondrial function as early as 3 hours after exposure with IPEC and MPEK cells. These data suggest that textile dyes can influence cell viability and possibly cause inflammation as a starting point to dysregulate the immune system. Supported by NCSU-DSA}, number={1}, journal={JOURNAL OF IMMUNOLOGY}, author={Lorenz-Cortes, Lizette and Vinueza-Benitez, Nelson}, year={2024}, month={May} }