@article{nierobisz_sporer_strasburg_reed_velleman_ashwell_felts_mozdziak_2012, title={Differential expression of genes characterizing myofibre phenotype}, volume={43}, ISSN={["1365-2052"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84859745099&partnerID=MN8TOARS}, DOI={10.1111/j.1365-2052.2011.02249.x}, abstractNote={Summary}, number={3}, journal={ANIMAL GENETICS}, author={Nierobisz, L. S. and Sporer, K. R. B. and Strasburg, G. M. and Reed, K. M. and Velleman, S. G. and Ashwell, C. M. and Felts, J. V. and Mozdziak, P. E.}, year={2012}, month={Jun}, pages={298–308} }
 @article{nierobisz_mcfarland_mozdziak_2011, title={MitoQ(10) induces adipogenesis and oxidative metabolism in myotube cultures}, volume={158}, ISSN={["1879-1107"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-78650173412&partnerID=MN8TOARS}, DOI={10.1016/j.cbpb.2010.10.003}, abstractNote={Coenzyme Q(10) (CoQ(10)) plays an essential role in determination of mitochondrial membrane potential and substrate utilization in all metabolically important tissues. The objective of the present study was to investigate the effect of Coenzyme Q analog (MitoQ(10)) on oxidative phenotype and adipogenesis in myotubes derived from fast-glycolytic Pectoralis major (PM) and slow-oxidative Anterior latissimus dorsi (ALD) muscles of the turkey (Meleagris gallopavo). The myotubes were subjected to the following treatments: fusion media alone, fusion media+125 nM MitoQ(10), and 500 nM MitoQ(10). Lipid accumulation was visualized by Oil Red O staining and quantified by measuring optical density of extracted lipid at 500 nm. Quantitative Real-Time PCR was utilized to quantify the expression levels of peroxisome proliferator-activated receptor (PPARγ) and PPARγ co-activator-1α (PGC-1α). MitoQ(10) treatment resulted in the highest (P<0.05) lipid accumulation in PM myotubes. MitoQ(10) up-regulated genes controlling oxidative mitochondrial biogenesis and adipogenesis in PM myotube cultures. In contrast, MitoQ(10) had a limited effect on adipogenesis and down-regulated oxidative metabolism in ALD myotube cultures. Differential response to MitoQ(10) treatment may be dependent on the cellular redox state. MitoQ(10) likely controls a range of metabolic pathways through its differential regulation of gene expression levels in myotubes derived from fast-glycolytic and slow-oxidative muscles.}, number={2}, journal={COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY}, author={Nierobisz, Lidia S. and McFarland, Douglas C. and Mozdziak, Paul E.}, year={2011}, month={Feb}, pages={125–131} }
 @article{nierobisz_hentz_felts_mozdziak_2010, title={Fiber Phenotype and Coenzyme Q(10) Content in Turkey Skeletal Muscles}, volume={192}, ISSN={["1422-6421"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-78649321430&partnerID=MN8TOARS}, DOI={10.1159/000319550}, abstractNote={Phenotypical differences between muscle fibers are associated with a source of cellular energy. Coenzyme Q<sub>10</sub> (CoQ<sub>10</sub>) is a major component of the mitochondrial oxidative phosphorylation process, and it significantly contributes to the production of cellular energy in the form of ATP. The objective of this study was to determine the relationship between whole-tissue CoQ<sub>10</sub> content, mitochondrial CoQ<sub>10</sub> content, mitochondrial protein, and muscle phenotype in turkeys. Four specialized muscles (anterior latissimus dorsi, ALD; posterior latissimus dorsi, PLD; pectoralis major, PM, and biceps femoris, BF) were evaluated in 9- and 20-week-old turkey toms. The amount of muscle mitochondrial protein was determined using the Bradford assay and CoQ<sub>10</sub> content was measured using HPLC-UV. The amount of mitochondrial protein relative to total protein was significantly lower (p < 0.05) at 9 compared to 20 weeks of age. All ALD fibers stained positive for anti-slow (S35) MyHC antibody. The PLD and PM muscle fibers revealed no staining for slow myosin heavy chain (S35 MyHC), whereas half of BF muscle fibers exhibited staining for S35 MyHC at 9 weeks and 70% at 20 weeks of age. The succinate dehydrogenase (SDH) staining data revealed that SDH significantly increases (p < 0.05) in ALD and BF muscles and significantly decreases (p < 0.05) in PLD and PM muscles with age. The study reveals age-related decreases in mitochondrial CoQ<sub>10</sub> content in muscles with fast/glycolytic profile, and demonstrates that muscles with a slow/oxidative phenotypic profile contain a higher proportion of CoQ<sub>10</sub> than muscles with a fast/glycolytic phenotypic profile.}, number={6}, journal={CELLS TISSUES ORGANS}, author={Nierobisz, L. S. and Hentz, N. G. and Felts, J. V. and Mozdziak, P. E.}, year={2010}, pages={382–394} }
 @article{nierobisz_felts_mozdziak_2009, title={Apoptosis and macrophage infiltration occur simultaneously and present a potential sign of muscle injury in skeletal muscle of nutritionally compromised, early post-hatch turkeys}, volume={153}, ISSN={["1879-1107"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-63249099336&partnerID=MN8TOARS}, DOI={10.1016/j.cbpb.2009.01.015}, abstractNote={Physical stress and malnutrition may cause elimination of myonuclei and produce inflammatory response in muscle. The objective of this study was to histochemically determine the association of apoptosis and/or macrophage infiltration with changes in muscle satellite cell mitotic activity in pectoralis thoracicus muscle of early post-hatch turkey toms. Feed-deprived birds and birds provided with three different levels of crude protein and amino acids (0.88 NRC, 1.00 NRC, and 1.12 NRC) were used in this model. The number of apoptotic nuclei was significantly elevated (P<0.05) and presence of macrophage infiltration was readily detectable in feed-deprived and 0.88 NRC treatment groups 72 h and 96 h post-hatch suggesting potential muscle injury and/or muscle remodeling. The number of apoptotic nuclei was the same (P>0.05), and there was no detectable macrophage infiltration present in birds placed on 1.00 NRC and 1.12 NRC diet 72 h, 96 h, and 120 h post-hatch. At 120 h post-hatch, feed-deprived and 0.88 NRC birds were characterized by no detectable levels of macrophage infiltration and a significant drop (P<0.05) in apoptotic nuclei. Understanding mechanisms that correlate early nutrition with skeletal muscle growth and development may present a useful tool in optimizing muscle health and improving meat quality and yield.}, number={1}, journal={COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY}, author={Nierobisz, L. S. and Felts, J. V. and Mozdziak, P. E.}, year={2009}, month={May}, pages={61–65} }
 @misc{nierobisz_mozdziak_2008, title={Factors influencing satellite cell activity during skeletal muscle development in avian and mammalian species}, volume={21}, ISSN={["1976-5517"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-43749098340&partnerID=MN8TOARS}, DOI={10.5713/ajas.2008.r.02}, abstractNote={Avian and mammalian skeletal muscles exhibit a remarkable ability to adjust to physiological stressors induced by growth, exercise, injury and disease. The process of muscle recovery following injury and myonuclear accretion during growth is attributed to a small population of satellite cells located beneath the basal lamina of the myofiber. Several metabolic factors contribute to the activation of satellite cells in response to stress mediated by illness, injury or aging. This review will describe the regenerative properties of satellite cells, the processes of satellite cell activation and highlight the potential role of satellite cells in skeletal muscle growth, tissue engineering and meat production.}, number={3}, journal={ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES}, author={Nierobisz, Lidia S. and Mozdziak, Paul E.}, year={2008}, month={Mar}, pages={456–464} }
 @article{nierobisz_felts_mozdziak_2007, title={The effect of early dietary amino acid levels on muscle satellite cell dynamics in turkeys}, volume={148}, ISSN={["1879-1107"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-35448939699&partnerID=MN8TOARS}, DOI={10.1016/j.cbpb.2007.06.006}, abstractNote={Understanding the relationship between nutrition and satellite cell activity will be beneficial in obtaining optimal muscle growth and meat production. The objective of this study was to evaluate the effect of early post-hatch levels of dietary amino acids ± 0.88 NRC, 1.00 NRC, and 1.12 NRC), and feed deprivation on the satellite cell mitotic activity, pectoralis thoracicus muscle weight, and body weight of male turkeys (Meleagris gallopavo). Birds from each treatment were injected with 5-bromo-2′-deoxyuridine (BrdU) to label mitotically active cells. The right pectoralis thoracicus was harvested 1 h after BrdU injection for immunohistochemical and myofiber diameter analysis. On the third day post-hatch, satellite cell mitotic activity was the highest (P < 0.05) in the 0.88 NRC amino acid treatment group and the lowest (P < 0.05) in the feed-deprived group. On the fourth day post-hatch, feed-deprived birds exhibited the lowest (P < 0.05) satellite cell mitotic activity and muscle weight. At 140 days of age, there were no significant differences (P > 0.05) between treatments in body weight or pectoralis thoracicus muscle weight. Research evaluating species-related differences in apoptotic events and in genes regulating cell proliferation may be necessary to devise feeding strategies aimed at obtaining optimal pectoralis thoracicus muscle yield at market age.}, number={3}, journal={COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY}, author={Nierobisz, L. S. and Felts, V. and Mozdziak, P. E.}, year={2007}, month={Nov}, pages={286–294} }