@article{stoeker_nordone_gunderson_zhang_kajikawa_lavoy_miller_klaenhammer_dean_2011, title={Assessment of Lactobacillus gasseri as a Candidate Oral Vaccine Vector}, volume={18}, ISSN={["1556-6811"]}, DOI={10.1128/cvi.05277-11}, abstractNote={Lactobacillus species are commensal bacteria that have long been recognized as probiotic microbes and are generally regarded as safe (GRAS) for human consumption. We have investigated the use of L. gasseri as a vaccine vector for oral immunization against mucosal pathogens. Recent research has shown that the immune response to different lactobacilli can vary widely depending on the species or subspecies of Lactobacillus being studied. While some lactobacilli seem to induce oral tolerance, others induce an adaptive immune response. This study characterized the systemic and mucosal immune response to wild-type and genetically modified L. gasseri. L. gasseri primarily activates TLR2/6, with additional activation through the TLR2 homodimer. To expand the Toll-like receptor (TLR) activation profile of L. gasseri and the immunogenicity of the vector, a plasmid containing fliC, the gene encoding bacterial flagellin, was introduced which resulted in the strong activation of TLR5. The treatment of human myeloid dendritic cells with recombinant lactobacilli expressing flagellin triggered phenotypic maturation and the release of proinflammatory cytokines. In contrast, bacterial treatment also resulted in a statistically significant increase in IL-10 production. In vivo studies established that treatment with L. gasseri led to a diversification of B-cell populations in the lamina propria of the murine colon. Furthermore, treatment with genetically modified L. gasseri led to a significant decrease in the percentage of FoxP3(+) colonic lymphocytes. Taken together, these data clarify the interaction of L. gasseri with the host immune system and support further investigation of the in vivo immunogenicity of L. gasseri expressing both flagellin and candidate vaccine antigens.}, number={11}, journal={CLINICAL AND VACCINE IMMUNOLOGY}, author={Stoeker, Laura and Nordone, Shila and Gunderson, Sara and Zhang, Lin and Kajikawa, Akinobu and LaVoy, Alora and Miller, Michael and Klaenhammer, Todd R. and Dean, Gregg A.}, year={2011}, month={Nov}, pages={1834–1844} }
 @article{tsai_cook_findley_miller_2011, title={North Carolina Ferry System A Study}, url={http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000299720000013&KeyUID=WOS:000299720000013}, DOI={10.3141/2216-12}, abstractNote={The North Carolina Department of Transportation Ferry Division faces several challenges. Recent U.S. Coast Guard (USCG) regulations increasing the minimum crew size on vessels carrying more than 149 passengers require the North Carolina Ferry System (NCFS) to add 79 employees. Difficulty in finding additional funds and certified crew to meet these regulations, coupled with reduced NCFS financial allocations from recent state budget cuts, resulted in a reduced number of ferry sailings on some routes during the peak operation period in the summer of 2009. In addition, NCFS experienced challenges from ferry service outages that resulted from the need to operate aging ferry vessels, a lack of standby ferry vessels in case of breakdowns, and vessels out of service as a result of the increased frequency of USCG dry dock inspections. The NCFS must also contend with unreliable support vessels that have exceeded their useful lives and a lack of certified welders and marine electricians to support maintenance functions. However, these resource and operation challenges present an opportunity to analyze existing ferry operations and to explore future opportunities.}, number={2216}, journal={Transportation Research Record}, author={Tsai, Jeffrey and Cook, Thomas and Findley, Daniel and Miller, Michael}, year={2011}, pages={108–115} }
 @article{miller_2008, title={Planning for enrollment growth using land use data to determine future school sites}, number={2074}, journal={Transportation Research Record}, author={Miller, M. A.}, year={2008}, pages={12–20} }