Works (50)

Updated: April 5th, 2024 03:58

2023 article

Identifying the enzyme responsible for initiating aerobic acetylene metabolism in Rhodococcus rhodochrous ATCC 33258

JOURNAL OF BIOLOGICAL CHEMISTRY, Vol. 299, pp. S369–S369.

By: J. Bingham, M. Hyman*, S. Montoya, M. Goshe* & C. Gracieux-Singleton

Sources: Web Of Science, NC State University Libraries
Added: September 11, 2023

2023 journal article

Tizoxanide Antiviral Activity on Dengue Virus Replication

VIRUSES-BASEL, 15(3).

By: K. Yamamoto*, K. Blackburn n, M. Goshe n, D. Brown n, E. Migoswski*, I. Campanhon*, M. Moreira*, D. Ferreira n, M. Soares*

author keywords: antiviral; tizoxanide; dengue virus; virucidal activity
MeSH headings : Chlorocebus aethiops; Animals; Humans; Antiviral Agents / pharmacology; Antiviral Agents / therapeutic use; Vero Cells; Dengue / drug therapy; Dengue Virus / genetics; Proteomics; Virus Replication
TL;DR: TIZ is a promising therapeutic molecule for the treatment of DENV-2 infections and the study of the protein profile of infected not-treated and infected-treated Vero cells showed that TIZ interferes with cellular processes such as intracellular trafficking and vesicle-mediated transport and post-translational modifications when added after infection. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: April 17, 2023

2021 article

Identification of multiple proteins whose interaction with mannitol dehydrogenase is induced by salicylic acid: Implications for unconventional secretion

Ho, T. C., Blackburn, R. K., Goshe, M. B., & Williamson, J. D. (2021, September 24). PROTEOMICS.

By: T. Ho n, R. Blackburn n, M. Goshe n & J. Williamson n

author keywords: database; mass spectrometry; plant pathogen interaction; protein-protein interaction; proteomics; unconventional protein secretion
MeSH headings : Arabidopsis / metabolism; Arabidopsis Proteins / metabolism; Gene Expression Regulation, Plant; Mannitol Dehydrogenases / genetics; Mannitol Dehydrogenases / metabolism; Plants / metabolism; Proteins; Salicylic Acid / pharmacology
TL;DR: High‐quality databases describing changes in MTD‐interacting proteins following SA treatment of Arabidopsis thaliana cells expressing MTD are presented. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: October 4, 2021

2020 journal article

Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis

NATURE COMMUNICATIONS, 11(1).

By: P. Dinh n, D. Paudel n, H. Brochu n, K. Popowski n, M. Gracieux n, J. Cores n, K. Huang n, M. Hensley n ...

MeSH headings : Administration, Inhalation; Alveolar Epithelial Cells / drug effects; Alveolar Epithelial Cells / metabolism; Alveolar Epithelial Cells / pathology; Animals; Apoptosis / drug effects; Bleomycin / toxicity; Cell Proliferation; Disease Models, Animal; Exosomes / metabolism; Exosomes / transplantation; Humans; Idiopathic Pulmonary Fibrosis / chemically induced; Idiopathic Pulmonary Fibrosis / metabolism; Idiopathic Pulmonary Fibrosis / pathology; Idiopathic Pulmonary Fibrosis / therapy; Lung / cytology; Lung / metabolism; Lung Injury / chemically induced; Lung Injury / metabolism; Lung Injury / pathology; Lung Injury / therapy; Mesenchymal Stem Cells / metabolism; Mice; Myofibroblasts / cytology; Proteomics; Silicon Dioxide / toxicity; Spheroids, Cellular / metabolism
TL;DR: It is shown that the secretome and exosomes of lung spheroid cells is effective as inhalation treatment in rodent models of lung injury and fibrosis and superior to the counterparts derived from mesenchymal stem cells. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: March 23, 2020

2020 journal article

Quantitative proteomic analysis of the tizoxanide effect in vero cells

SCIENTIFIC REPORTS, 10(1).

By: K. Yamamoto*, K. Blackburn n, E. Migowski*, M. Goshe n, D. Brown n, D. Ferreira n, M. Soares*

MeSH headings : Animals; Anti-Infective Agents / pharmacology; Chlorocebus aethiops; Fatty Acid Synthases / genetics; Fatty Acid Synthases / metabolism; Proteome / drug effects; Proteome / genetics; Proteome / metabolism; Ribonucleoproteins / genetics; Ribonucleoproteins / metabolism; Thiazoles / pharmacology; Vero Cells
TL;DR: The broad antimicrobial range of TIZ points towards its overall effect in lowering cell metabolism and RNA processing and modification, and the decreased levels of FASN, HNRNPH and HNRNPK with the treatment appear to be important for antiviral activity. (via Semantic Scholar)
Source: Web Of Science
Added: October 5, 2020

2019 journal article

Proteomic method to extract, concentrate, digest and enrich peptides from fossils with coloured (humic) substances for mass spectrometry analyses

ROYAL SOCIETY OPEN SCIENCE, 6(8).

By: E. Schroeter n, K. Blackburn n, M. Goshe n & M. Schweitzer n

author keywords: palaeoproteomics; fossils; humic substances; protein; mass spectrometry; moa
TL;DR: A method combining multiple recent advances in extraction protocols to both concentrate proteins from fossil specimens with high humic content and remove humics, producing clean samples easily analysed by mass spectrometry (MS). (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: September 23, 2019

2019 journal article

Public questions spur the discovery of new bacterial species associated with lignin bioconversion of industrial waste

ROYAL SOCIETY OPEN SCIENCE, 6(3).

By: S. Mathews*, M. Epps*, R. Blackburn n, M. Goshe n, A. Grunden n & R. Dunn n

author keywords: greenhouse camel cricket; hide beetle; Cedecea lapagei; lignocellulose; secretome
TL;DR: This work demonstrates a novel source of lignocellulose-degrading bacteria and introduces an effective workflow to identify bacterial enzymes for transforming industrial waste into value-added products and suggests the value of ecologically guided discovery of novel organisms. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: May 28, 2019

2019 journal article

Targeted Capture of Chinese Hamster Ovary Host Cell Proteins: Peptide Ligand Discovery

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20(7).

By: R. Lavoie n, A. Fazio n, R. Blackburn n, M. Goshe n, R. Carbonell n & S. Menegatti n

author keywords: therapeutic antibodies; host cell proteins; protein purification; peptide ligands
MeSH headings : Animals; CHO Cells; Chromatography, Affinity; Chromatography, Liquid; Cricetinae; Cricetulus; Humans; Peptides / chemistry; Peptides / isolation & purification; Proteomics / methods
TL;DR: Development of synthetic peptide ligands capable of capturing a broad spectrum of Chinese hamster ovary (CHO) HCPs with a combination of peptide species that allow for advanced mixed-mode binding is described. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: May 6, 2019

2018 journal article

Sucrose Nonfermenting 1-Related Protein Kinase 1 Phosphorylates a Geminivirus Rep Protein to Impair Viral Replication and Infection

PLANT PHYSIOLOGY, 178(1), 372–389.

By: W. Shen n, B. Bobay*, L. Greeley n, M. Reyes n, C. Rajabu n, R. Blackburn n, M. Dallas n, M. Goshe n, J. Ascencio-Ibanez n, L. Hanley-Bowdoin n

MeSH headings : Amino Acid Sequence; Begomovirus / genetics; Begomovirus / metabolism; Begomovirus / physiology; Host-Pathogen Interactions; Solanum lycopersicum / enzymology; Solanum lycopersicum / virology; Mutation; Phosphorylation; Plant Diseases / virology; Plant Proteins / genetics; Plant Proteins / metabolism; Protein Binding; Protein Domains; Protein Serine-Threonine Kinases / genetics; Protein Serine-Threonine Kinases / metabolism; Sequence Homology, Amino Acid; Serine / genetics; Serine / metabolism; Viral Proteins / chemistry; Viral Proteins / genetics; Viral Proteins / metabolism; Virus Replication
TL;DR: It is established that SnRK1 phosphorylates Rep and interferes with geminivirus replication and infection, underscoring the emerging role for SnRk1 in the host defense response against plant pathogens. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 16, 2018

2017 journal article

Autophosphorylation-based Calcium (Ca2(+)) Sensitivity Priming and Ca2(+)/Calmodulin Inhibition of Arabidopsis thaliana Ca2(+)-dependent Protein Kinase 28 (CPK28)

JOURNAL OF BIOLOGICAL CHEMISTRY, 292(10), 3988–4002.

By: K. Bender*, R. Blackburn n, J. Monaghan*, P. Derbyshire*, F. Menke*, C. Zipfel*, M. Goshe n, R. Zielinski*, S. Huber*

MeSH headings : Amino Acid Sequence; Arabidopsis / drug effects; Arabidopsis / growth & development; Arabidopsis / metabolism; Arabidopsis Proteins / metabolism; Calcium / pharmacology; Calmodulin / pharmacology; Gene Expression Regulation, Plant / drug effects; Kinetics; Phosphorylation / drug effects; Protein Binding; Protein Conformation; Protein Interaction Domains and Motifs; Protein Kinases / chemistry; Protein Kinases / metabolism; Sequence Homology, Amino Acid
TL;DR: In situ autophosphorylation of CPK28 was demonstrated to have enhanced kinase activity at physiological concentrations of Ca2+ compared with the dephosphorylated protein, suggesting that autoph phosphorylation functions to primeCPK28 forCa2+ activation and might also allow CPK 28 to remain active when Ca2- levels are low. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Quantitative Proteomic Analysis of Human Airway Cilia Identifies Previously Uncharacterized Proteins of High Abundance

JOURNAL OF PROTEOME RESEARCH, 16(4), 1579–1592.

author keywords: cilia; axoneme; mass spectrometry; proteomics; airway; dynein; tubulin; radial spoke
MeSH headings : Axoneme / genetics; Cilia / genetics; Dyneins / genetics; Dyneins / isolation & purification; Gene Expression Regulation; Humans; Proteins / genetics; Proteins / isolation & purification; Proteome / genetics; Proteomics; Tubulin / genetics; Tubulin / isolation & purification
TL;DR: These studies have provided the first quantitative analysis of the ciliary proteome and have identified and characterized several previously unknown proteins as major constituents of human airway cilia. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2017 journal article

The Arabidopsis Leucine-Rich Repeat Receptor Kinase BIR3 Negatively Regulates BAK1 Receptor Complex Formation and Stabilizes BAK1

PLANT CELL, 29(9), 2285–2303.

By: J. Imkampe*, T. Halter*, S. Huang*, S. Schulze*, S. Mazzotta*, N. Schmidt*, R. Manstretta*, S. Postel* ...

MeSH headings : Arabidopsis / drug effects; Arabidopsis / metabolism; Arabidopsis Proteins / metabolism; Brassinosteroids / metabolism; Cell Death / drug effects; Flagellin / pharmacology; Leucine-Rich Repeat Proteins; Ligands; Membrane Proteins / metabolism; Mutation / genetics; Pathogen-Associated Molecular Pattern Molecules / metabolism; Phenotype; Protein Binding / drug effects; Protein Serine-Threonine Kinases / metabolism; Protein Stability / drug effects; Proteins / metabolism; Signal Transduction
TL;DR: It is shown that BAK1-related pathways such as innate immunity and cell death control are affected by BIR3 in Arabidopsis thaliana, and Bir3 also has a strong negative impact on BR signaling. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Gatekeeper Tyrosine Phosphorylation of SYMRK Is Essential for Synchronizing the Epidermal and Cortical Responses in Root Nodule Symbiosis

PLANT PHYSIOLOGY, 171(1), 71–81.

By: S. Saha*, A. Paul*, L. Herring n, A. Dutta*, A. Bhattacharya*, S. Samaddar*, M. Goshe n, M. DasGupta*

MeSH headings : Amino Acid Sequence; Carrier Proteins / genetics; Carrier Proteins / metabolism; Fabaceae / metabolism; Gene Expression Regulation, Plant; Genes, Plant; Mutagenesis; Mutation; Phenotype; Phosphoamino Acids / analysis; Phosphorylation; Plant Epidermis; Plant Proteins / genetics; Plant Proteins / metabolism; Plant Root Nodulation; Plant Roots / metabolism; Plant Roots / microbiology; Protein Kinases / genetics; Protein Kinases / metabolism; Rhizobium / physiology; Root Nodules, Plant / enzymology; Root Nodules, Plant / genetics; Root Nodules, Plant / metabolism; Symbiosis; Tyrosine / metabolism
TL;DR: It is shown that substituting gatekeeper with nonphosphorylatable residues like Phe or Ala significantly affected autophosphorylation on selected targets on activation segment/αEF and β3-αC loop of SYMRK. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Development of a tandem affinity phosphoproteomic method with motif selectivity and its application in analysis of signal transduction networks

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 988, 166–174.

By: L. Herring n, K. Grant*, K. Blackburn n, J. Haugh n & M. Goshe n

author keywords: Phosphopeptide enrichment; Liquid chromatography; Mass spectrometry; Motif analysis; Pathway analysis
MeSH headings : Amino Acid Sequence; Chromatography, Liquid / methods; Molecular Sequence Data; Phosphopeptides / analysis; Phosphopeptides / chemistry; Proteomics / methods; Signal Transduction / physiology; Tandem Mass Spectrometry / methods
TL;DR: A multi-dimensional method incorporating electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) with tandem IMAC/TiO2 enrichment for subsequent phosphopeptide identification by LC/MS/MS and biological functions that were over-represented at each PDGF stimulation time point illustrate the feasibility of this approach in quantitative phosphoproteomic studies. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2015 journal article

Performing protein crosslinking using gas-phase cleavable chemical crosslinkers and liquid chromatography-tandem mass spectrometry

METHODS, 89, 64–73.

By: A. Argo n, C. Shi n, F. Liu n & M. Goshe n

author keywords: Protein structure; Protein-protein interactions; Crosslinking; Gas-phase cleavable crosslinkers; CID-cleavable crosslinkers; Mass spectrometry
MeSH headings : Animals; Cattle; Chromatography, Liquid / methods; Cross-Linking Reagents / chemistry; Cross-Linking Reagents / metabolism; Protein Binding / physiology; Serum Albumin, Bovine / analysis; Serum Albumin, Bovine / metabolism; Tandem Mass Spectrometry / methods
TL;DR: The CID-CXL-MS/MS approach can be used as a tool for biological research for studying protein structure when integrated with data obtained using other techniques, such as NMR, X-ray crystallography, and cryo-electron microscopy, or extended to the study of protein-protein interactions in physiological environments. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Data-driven modeling reconciles kinetics of ERK phosphorylation, localization, and activity states

MOLECULAR SYSTEMS BIOLOGY, 10(1).

By: S. Ahmed n, K. Grant n, L. Edwards n, A. Rahman n, M. Cirit n, M. Goshe n, J. Haugh n

author keywords: growth factor receptors; mitogen-activated protein kinases; negative feedback; nucleocytoplasmic shuttling; mathematical model
MeSH headings : Active Transport, Cell Nucleus; Animals; Extracellular Signal-Regulated MAP Kinases / chemistry; Extracellular Signal-Regulated MAP Kinases / metabolism; Feedback, Physiological; Kinetics; Mice; Models, Theoretical; NIH 3T3 Cells; Phosphorylation; Protein Transport / genetics; Receptors, Growth Factor / genetics; Signal Transduction / genetics
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2014 journal article

Protein Molecular Data from Ancient (>1 million years old) Fossil Material: Pitfalls, Possibilities and Grand Challenges

Analytical Chemistry, 86(14), 6731–6740.

By: M. Schweitzer*, E. Schroeter & M. Goshe*

MeSH headings : Animals; Bone and Bones; Collagen / analysis; Collagen / chemistry; Dinosaurs; Fossils; Hydroxyproline / analysis; Keratins / analysis; Keratins / chemistry; Mass Spectrometry / methods; Proteins / analysis; Proteomics / methods
TL;DR: The challenges to recovery of proteinaceous components from fossils, and the need for new sample preparation techniques, analytical methods, and bioinformatics to optimize and fully utilize the great potential of information locked in the fossil record are discussed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2014 journal article

SnRK1 Phosphorylation of AL2 Delays Cabbage Leaf Curl Virus Infection in Arabidopsis

JOURNAL OF VIROLOGY, 88(18), 10598–10612.

By: W. Shen n, M. Dallas n, M. Goshe n & L. Hanley-Bowdoin n

MeSH headings : Amino Acid Sequence; Arabidopsis / enzymology; Arabidopsis / genetics; Arabidopsis / virology; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Begomovirus / chemistry; Begomovirus / classification; Begomovirus / genetics; Begomovirus / metabolism; Host-Pathogen Interactions; Molecular Sequence Data; Phylogeny; Plant Diseases / virology; Protein Binding; Protein Kinases / genetics; Protein Kinases / metabolism; Transcription Factors / genetics; Transcription Factors / metabolism; Viral Proteins / chemistry; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR: It is shown that the host protein kinase SnRK1, a central regulator of energy balance and nutrient metabolism in plants, phosphorylates serine-109 in AL2 proteins of three subgroups of New World begomoviruses, resulting in a delay in viral DNA accumulation and symptom appearance. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

Autophosphorylation of gatekeeper tyrosine by symbiosis receptor kinase

FEBS LETTERS, 587(18), 2972–2979.

By: S. Samaddar*, A. Dutta*, S. Sinharoy*, A. Paul*, A. Bhattacharya*, S. Saha*, K. Chien n, M. Goshe n, M. DasGupta*

author keywords: Autophosphorylation; Gatekeeper residue; Receptor-like kinase; Symbiosis receptor kinase; Tyrosine kinase-like
MeSH headings : Amino Acid Motifs; Arachis / genetics; Arachis / metabolism; Catalytic Domain; Cell Line; Gene Expression Regulation, Plant; Mutation; Phosphorylation; Plant Proteins / genetics; Plant Proteins / metabolism; Protein Binding; Receptor Protein-Tyrosine Kinases / genetics; Receptor Protein-Tyrosine Kinases / metabolism; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Signal Transduction; Tyrosine / genetics; Tyrosine / metabolism
TL;DR: It is hypothesize gatekeeper phosphorylation to be associated with synthesis/maturation of AhSYMRK and this phenomenon may be prevalent among RLKs. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Chemical crosslinking and LC/MS analysis to determine protein domain orientation: Application to AbrB

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 431(2), 253–257.

By: A. Olson*, F. Liu*, A. Tucker*, M. Goshe* & J. Cavanagh*

author keywords: NMR; Solution structure; Chemical crosslinking; Domain orientation; AbrB; Gas-phase cleavable
MeSH headings : Amino Acid Sequence; Bacterial Proteins / chemistry; Chromatography, Liquid; Cross-Linking Reagents / chemistry; DNA-Binding Proteins / chemistry; Molecular Sequence Data; Protein Structure, Tertiary; Tandem Mass Spectrometry; Transcription Factors / chemistry
TL;DR: This work solved the NMR structures of both domains independently and utilized gas-phase cleavable chemical crosslinking and LC/MS(n) analysis to correctly position the domains within the full tetrameric AbrB protein structure. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Identification and Functional Analysis of Tomato BRI1 and BAK1 Receptor Kinase Phosphorylation Sites

PLANT PHYSIOLOGY, 163(1), 30–42.

By: V. Bajwa, X. Wang*, R. Blackburn n, M. Goshe n, S. Mitra*, E. Williams*, G. Bishop*, S. Krasnyanski* ...

MeSH headings : Binding Sites; Kinetics; Solanum lycopersicum / enzymology; Phosphorylation; Plant Proteins / chemistry; Plant Proteins / metabolism; Protein Serine-Threonine Kinases / chemistry; Protein Serine-Threonine Kinases / metabolism; Signal Transduction
TL;DR: Liquid chromatography-tandem mass spectrometry was used to identify multiple in vitro phosphorylation sites of the tomato BRI1 and BAK1 cytoplasmic domains and found that tomato TRIP1 is a substrate of both tomato Bri1 andBAK1 kinases in vitro. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2012 journal article

An enhanced protein crosslink identification strategy using CID-cleavable chemical crosslinkers and LC/MSn analysis

PROTEOMICS, 12(3), 401–405.

By: F. Liu n, C. Wu*, J. Sweedler* & M. Goshe n

author keywords: CID-cleavable crosslinker; Crosslinking; Mass measurement accuracy; Multistage CID; Protein-protein interactions; Technology
MeSH headings : Algorithms; Chromatography, Liquid / methods; Complex Mixtures / analysis; Databases, Protein; Hemoglobins / chemistry; Humans; Mass Spectrometry / methods; Peptides / analysis; Peptides / metabolism; Proteins / analysis; Proteins / metabolism
TL;DR: A novel two‐step LC/MSn strategy to effectively and confidently identify numerous crosslinked peptides from complex mixtures that enables unequivocal crosslink peptide selection and identification on the basis of mass measurement accuracy, high resolving power, and the unique fragmentation pattern of each cross linked peptide. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Proteomic and Phosphoproteomic Analysis of Chicken Embryo Fibroblasts Infected with Cell Culture-Attenuated and Vaccine Strains of Marek's Disease Virus

JOURNAL OF PROTEOME RESEARCH, 11(12), 5663–5677.

By: K. Chien n, K. Blackburn n, H. Liu n & M. Goshe n

author keywords: proteomics; phosphoproteomics; liquid chromatography; mass spectrometry; Marek's Disease Virus; phosphorylation
MeSH headings : Amino Acid Sequence; Animals; Cells, Cultured; Chick Embryo; Chromatography, Affinity / methods; Eukaryotic Initiation Factor-4E / metabolism; Fibroblasts / metabolism; Fibroblasts / virology; Herpesvirus 2, Gallid / classification; Herpesvirus 2, Gallid / pathogenicity; Marek Disease; Mass Spectrometry / methods; Molecular Sequence Data; Multiprotein Complexes / analysis; Multiprotein Complexes / metabolism; Peptide Chain Initiation, Translational; Phosphopeptides / analysis; Phosphopeptides / metabolism; Phosphoproteins / analysis; Phosphoproteins / metabolism; Phosphorylation; Proteome / analysis; Proteome / metabolism; Proteomics / methods; Transcription, Genetic; Viral Plaque Assay
TL;DR: A kinase-dependent pathway to regulate phosphorylation of 4E-BP1 to modulate assembly of the protein translation initiation complex was revealed and provided new insight for future functional and biochemical characterization of specific proteins involved in MDV pathogenesis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2011 review

Advances in qualitative and quantitative plant membrane proteomics

[Review of ]. PHYTOCHEMISTRY, 72(10), 1040–1060.

By: U. Kota n & M. Goshe n

author keywords: Membrane proteomics; Liquid chromatography; Mass spectrometry; Quantitation; Quantification; Stable isotope-coded; Label-free
MeSH headings : Cell Membrane / chemistry; Cell Membrane / metabolism; Organelles / chemistry; Organelles / metabolism; Plant Proteins / analysis; Plant Proteins / metabolism; Protein Processing, Post-Translational; Proteomics
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Development and Application of a Phosphoproteomic Method Using Electrostatic Repulsion-Hydrophilic Interaction Chromatography (ERLIC), IMAC, and LC-MS/MS Analysis to Study Marek's Disease Virus Infection

JOURNAL OF PROTEOME RESEARCH, 10(9), 4041–4053.

By: K. Chien n, H. Liu n & M. Goshe n

author keywords: phosphoproteomics; liquid chromatography; mass spectrometry; Marek's Disease Virus; phosphorylation
MeSH headings : Amino Acid Sequence; Animals; Avian Proteins / analysis; Avian Proteins / metabolism; Chick Embryo; Chromatography, Liquid; Fibroblasts; Host-Pathogen Interactions / physiology; Hydrophobic and Hydrophilic Interactions; Mardivirus; Marek Disease / immunology; Marek Disease / metabolism; Marek Disease / virology; Molecular Sequence Data; Peptide Fragments / analysis; Peptide Fragments / metabolism; Phosphopeptides / analysis; Phosphopeptides / metabolism; Proteomics / methods; Proteomics / standards; Reproducibility of Results; Static Electricity; Tandem Mass Spectrometry; Viral Proteins / analysis; Viral Proteins / metabolism
TL;DR: An effective separation and enrichment approach is developed to investigate the changes occurring in the phosphoproteome using electrostatic repulsion-hydrophilic interaction chromatography to fractionate peptides from chicken embryo fibroblast (CEF) digests and incorporating a subsequent IMAC enrichment step to selectively target phosphorylated peptides for LC-MS/MS analysis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Investigation of solubilization and digestion methods for microsomal membrane proteome analysis using data-independent LC-MSE

PROTEOMICS, 11(5), 898–911.

By: F. Mbeunkui n & M. Goshe n

author keywords: Bottom-up proteomics; Data-independent analysis; Denaturants; LC-MSE; Membrane proteomics; Technology
MeSH headings : Chromatography, Liquid; Guanidine / chemistry; Solanum lycopersicum / chemistry; Membrane Proteins / analysis; Membrane Proteins / chemistry; Membrane Proteins / metabolism; Methanol / chemistry; Microsomes / chemistry; Microsomes / enzymology; Peptide Fragments / analysis; Peptide Fragments / chemistry; Peptide Fragments / metabolism; Plant Roots / chemistry; Plant Roots / cytology; Proteome / analysis; Proteome / chemistry; Proteome / metabolism; Proteomics / methods; Solubility; Surface-Active Agents / chemistry; Tandem Mass Spectrometry; Trypsin / metabolism
TL;DR: The results reveal the complementary nature of each denaturant method, which can be used in an integrated approach to provide a more effective bottom‐up analysis of membrane proteomes than can be achieved using only a singleDenaturant. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Mass Spectrometric Detection of Targeting Peptide Bioconjugation to Red clover necrotic mosaic virus

BIOCONJUGATE CHEMISTRY, 22(10), 1970–1982.

By: R. Wang, D. Lockney*, M. Goshe* & S. Franzen*

MeSH headings : Amino Acid Sequence; Capsid / chemistry; Cross-Linking Reagents / chemistry; Drug Delivery Systems; Mass Spectrometry; Models, Molecular; Molecular Sequence Data; Peptides / chemistry; Tombusviridae / chemistry
TL;DR: Results show that heterobifunctional linkers may not be the most efficient method for attachment of peptides to plant virus capsids, and maleimide peptides were used to conjugate with the virus in a one-step reaction. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Combinatorial Electrostatic Collision-Induced Dissociative Chemical Cross-linking Reagents for Probing Protein Surface Topology

ANALYTICAL CHEMISTRY, 82(14), 6215–6223.

By: F. Liu n & M. Goshe n

MeSH headings : Amino Acid Sequence; Animals; Cattle; Chromatography, Liquid / methods; Circular Dichroism; Cross-Linking Reagents / chemistry; Dipeptides / chemistry; Hemoglobins / chemistry; Hemoglobins / metabolism; Molecular Sequence Data; Protein Structure, Tertiary; Proteins / chemistry; Serum Albumin, Bovine / chemistry; Serum Albumin, Bovine / metabolism; Static Electricity; Tandem Mass Spectrometry / methods
TL;DR: Overall, combinatorial application of SuDP and SuVP chemical labeling produces a set of unique interpeptid, intrapeptide, and dead-end cross-linked products that provides protein structural information according to its electrostatic surface topology which has the potential to be used to more comprehensively probe protein structure and dynamics. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Data-independent liquid chromatography/mass spectrometry (LC/MSE) detection and quantification of the secreted Apium graveolens pathogen defense protein mannitol dehydrogenase

Rapid Communications in Mass Spectrometry, 24(7), 1009–1016.

By: K. Blackburn n, F. Cheng n, J. Williamson n & M. Goshe n

MeSH headings : Amino Acid Sequence; Apium / drug effects; Apium / enzymology; Cell Culture Techniques; Chromatography, Liquid / methods; Electrophoresis, Polyacrylamide Gel; Immunoblotting; Mannitol Dehydrogenases / chemistry; Mannitol Dehydrogenases / genetics; Mannitol Dehydrogenases / metabolism; Molecular Sequence Data; Proteome / analysis; Proteome / drug effects; Proteome / metabolism; Salicylic Acid / pharmacology; Tandem Mass Spectrometry / methods
TL;DR: LC/MS(E) can be implemented as a discovery-driven MRM-based quantitative approach which can be used to reveal potential post-translational modifications, thus providing a new method in the area of gel-free and label-free proteomic analysis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: NC State University Libraries
Added: August 6, 2018

2010 journal article

Improving Protein and Proteome Coverage through Data-Independent Multiplexed Peptide Fragmentation

JOURNAL OF PROTEOME RESEARCH, 9(7), 3621–3637.

By: K. Blackburn n, F. Mbeunkui n, S. Mitra n, T. Mentzel n & M. Goshe n

author keywords: Proteomics; mass spectrometry; protein characterization; data-independent acquisition (DIA); data-dependent acquisition (DDA); protein identification; LC/MSE
MeSH headings : Amino Acid Sequence; Animals; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / metabolism; Artifacts; Cattle; Chromatography, Liquid / methods; Ions / chemistry; Solanum lycopersicum; Mass Spectrometry / methods; Molecular Sequence Data; Peptide Fragments / chemistry; Peptide Fragments / metabolism; Plant Leaves; Plant Proteins / chemistry; Plant Proteins / metabolism; Protein Kinases / chemistry; Protein Kinases / metabolism; Proteomics / methods; Serum Albumin, Bovine / chemistry; Serum Albumin, Bovine / metabolism; Trypsin / metabolism
TL;DR: The fundamental limitations of serial MS/MS interrogation are revealed and the advantages of parallel fragmentation by DIA for more comprehensive protein identification and characterization are revealed which holds promise for enhanced isoform and post-translational modification analysis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Proteomic and Bioinformatic Analysis of the Root-Knot Nematode Meloidogyne hapla: The Basis for Plant Parasitism

Journal of Proteome Research, 9(10), 5370–5381.

By: F. Mbeunkui n, E. Scholl n, C. Opperman n, M. Goshe n & D. Bird n

Contributors: F. Mbeunkui n, E. Scholl n, C. Opperman n, M. Goshe n & D. Bird n

author keywords: LC/MSE; plant-parasite; nematode; data-independent acquisition; computational proteome
MeSH headings : Animals; Chromatography, Liquid; Computational Biology / methods; Databases, Protein; Genome, Helminth / genetics; Helminth Proteins / genetics; Helminth Proteins / metabolism; Humans; Internet; Plant Roots / parasitology; Proteomics / methods; Tandem Mass Spectrometry; Tylenchoidea / genetics; Tylenchoidea / metabolism
TL;DR: To experimentally confirm the HapPep3 assignments using proteomics, a data-independent LC/MS(E) analysis to M. hapla protein extracts fractionated by SDS-PAGE was applied, and it was concluded that the identified proteins reflect a true and unbiased sampling of Happep3. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

2009 journal article

A role for hydrophobicity in a DielsAlder reaction catalyzed by pyridyl-modified RNA

NUCLEIC ACIDS RESEARCH, 37(9), 3074–3082.

By: K. Gagnon n, S. Ju n, M. Goshe n, E. Maxwell n & S. Franzen n

MeSH headings : Anthracenes / metabolism; Base Sequence; Catalysis; Ethylene Glycols / metabolism; Hydrophobic and Hydrophilic Interactions; Lactones; Molecular Sequence Data; Nucleic Acid Conformation; Pyridines / chemistry; RNA / chemistry; RNA / metabolism; RNA, Catalytic / chemistry; RNA, Catalytic / metabolism; Sesquiterpenes
TL;DR: It is shown that DA_TR96, the most active member of the DA22 RNA sequence family, which was selected with pyridyl-modified nucleotides, accelerates a cycloaddition reaction between anthracene and maleimide derivatives with high turnover, and suggests a key role for the hydrophobic effect in the catalytic mechanism. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

Absolute Protein Quantification by LC/MSE for Global Analysis of Salicylic Acid-induced Plant Protein Secretion Responses

JOURNAL OF PROTEOME RESEARCH, 8(1), 82–93.

By: F. Cheng n, K. Blackburn n, Y. Lin n, M. Goshe n & J. Williamson n

author keywords: Arabidopsis; secretome; liquid chromatography; mass spectrometry; MSE; data-independent acquisition; salicylic acid; pathogen; nonclassical protein secretion
MeSH headings : Arabidopsis / drug effects; Arabidopsis / metabolism; Chromatography, Liquid / methods; Computational Biology; Mass Spectrometry / methods; Plant Physiological Phenomena; Plant Proteins / metabolism; Proteins / metabolism; Proteome; Proteomics / methods; Salicylic Acid / metabolism; Salicylic Acid / pharmacology; Time Factors
TL;DR: A mass spectrometry, data-independent acquisition approach known as LC/MS (E) was used to assess temporal changes in the cell wall proteome in response to different levels of an endogenous inducer of plant disease defense responses, salicylic acid (SA). (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 review

Advances in Quantitative Mass Spectrometry Analysis: Weighing in on Isotope Coding and Label-Free Approaches for Expression and Functional Proteomics

[Review of ]. CURRENT ANALYTICAL CHEMISTRY, 5(2), 166–185.

By: K. Chien* & M. Goshe*

author keywords: Mass spectrometry; Quantitative proteomics; Phosphoproteomics; Isotope coding; Label-free quantitation; Absolute quantitation
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2009 journal article

An Efficient Organic Solvent Based Extraction Method for the Proteomic Analysis of Arabidopsis Plasma Membranes

JOURNAL OF PROTEOME RESEARCH, 8(6), 2752–2767.

By: S. Mitra n, B. Walters n, S. Clouse n & M. Goshe n

author keywords: Proteomics; mass spectrometry; liquid chromatography; strong cation exchanges; phase partitioning; membrane proteins; transporters; Arabidopsis; methanol
MeSH headings : Amino Acid Sequence; Arabidopsis / chemistry; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / isolation & purification; Arabidopsis Proteins / metabolism; Chemical Fractionation / methods; Chloroform; Chlorophyll; Chromatography, Ion Exchange; Databases, Protein; Membrane Proteins / chemistry; Membrane Proteins / isolation & purification; Membrane Proteins / metabolism; Methanol; Molecular Sequence Data; Peptide Fragments / chemistry; Peptide Fragments / metabolism; Protein Kinases / isolation & purification; Protein Kinases / metabolism; Proteomics / methods; Solvents / chemistry; Tandem Mass Spectrometry; Trypsin / metabolism
TL;DR: The combined use of methanol and chloroform provides an effective method to study membrane proteins and can be readily applied to other tissues and cells types for proteomic analysis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Mass Spectrometry Bioinformatics: Tools for Navigating the Proteomics Landscape

CURRENT ANALYTICAL CHEMISTRY, 5(2), 131–143.

By: K. Blackburn* & M. Goshe*

author keywords: Mass spectrometry; Peptide identification; Product ion spectrum; Database searching; Proteomics; Bioinformatics
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Tyrosine phosphorylation of the BRI1 receptor kinase emerges as a component of brassinosteroid signaling in Arabidopsis

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 106(2), 658–663.

By: M. Oh*, X. Wang*, U. Kota n, M. Goshe n, S. Clouse* & S. Huber*

MeSH headings : Arabidopsis; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Mutation; Phosphorylation; Plant Leaves; Protein Kinases / genetics; Protein Kinases / metabolism; Protein Serine-Threonine Kinases / metabolism; Signal Transduction; Tyrosine / metabolism
TL;DR: It is reported that recombinant cytoplasmic domains of BRI1 and BAK1 also autophosphorylate on tyrosine residues and thus are dual-specificity kinases, suggesting that tyosine signaling should be considered with other plant receptor kinases as well. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2008 journal article

A sequence-anchored genetic linkage map for the moss, Physcomitrella patens

Plant Journal, 56(5), 840–854.

By: J. Schaff, F. Mbeunkui, K. Blackburn, D. Bird & M. Goshe

Source: NC State University Libraries
Added: August 6, 2018

2008 journal article

Changes in the Gallus gallus proteorne induced by Marek's disease virus

JOURNAL OF PROTEOME RESEARCH, 7(10), 4346–4358.

By: M. Ramaroson n, J. Ruby n, M. Goshe n & H. Liu n

author keywords: Marek's disease virus; chicken proteome; mu rpLC/MS/MS
MeSH headings : Animals; Chickens / virology; Computational Biology; Herpesvirus 2, Gallid / metabolism; Humans; Mass Spectrometry; Molecular Sequence Data; Peptides / analysis; Phosphopeptides / analysis; Proteome
TL;DR: The results suggest that MDV infection may alter host cell biochemistry by perturbing the host's proteomic composition with respect to Marek's disease virus. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Characterization of a new family of protein kinases from Arabidopsis containing phosphoinositide 3/4-kinase and ubiquitin-like domains

BIOCHEMICAL JOURNAL, 409, 117–127.

By: R. Galvao n, U. Kota n, E. Soderblom n, M. Goshe n & W. Boss n

author keywords: phosphoinositide kinase (PIK); phosphorylation; proteasome regulatory particle non-ATPase 10 subunit (proteasome RPN10 subunit); protein kinase; ubiquitin fusion degradation (UFD); ubiquitin-like domain (UBL domain)
MeSH headings : Adaptor Proteins, Signal Transducing / chemistry; Arabidopsis / metabolism; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / metabolism; Catalysis; Class Ia Phosphatidylinositol 3-Kinase; Cloning, Molecular; DNA, Complementary / metabolism; Genetic Vectors; Humans; Phosphatidylinositol 3-Kinases / chemistry; Phosphatidylinositol 3-Kinases / metabolism; Phosphorylation; Phylogeny; Protein Kinases / chemistry; Protein Kinases / classification; Protein Structure, Tertiary; Recombinant Proteins / chemistry; Ubiquitin / chemistry; Ubiquitin / metabolism
TL;DR: In vitro analysis of AtPI4Kgamma4 indicates that it interacts directly with, and phosphorylates, two proteins involved in the ubiquitin-proteasome system, namely UFD1 (ubiquitin fusion degradation 1) and RPN10 (regulatory particle non-ATPase 10). (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Sequential transphosphorylation of the BRI1/BAK1 receptor kinase complex impacts early events in brassinosteroid signaling

DEVELOPMENTAL CELL, 15(2), 220–235.

By: X. Wang n, U. Kota n, K. He*, K. Blackburn n, J. Li*, M. Goshe n, S. Huber*, S. Clouse n

MeSH headings : Amino Acid Sequence; Arabidopsis / drug effects; Arabidopsis / enzymology; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / metabolism; Brassinosteroids; Cholestanols / metabolism; Cholestanols / pharmacology; Chromatography, Liquid; Flagellin / metabolism; Mass Spectrometry; Models, Biological; Molecular Sequence Data; Mutation / genetics; Phosphorylation / drug effects; Protein Kinases / chemistry; Protein Kinases / metabolism; Protein Serine-Threonine Kinases / chemistry; Protein Serine-Threonine Kinases / metabolism; Protein Structure, Tertiary; Signal Transduction / drug effects; Steroids, Heterocyclic / metabolism; Steroids, Heterocyclic / pharmacology; Substrate Specificity / drug effects
TL;DR: A sequential transphosphorylation model in which BRI1 controls signaling specificity by direct BR binding followed by substrate phosphorylation is proposed, which suggests both conservation and distinct differences between the molecular mechanisms regulating phosphorylated-dependent kinase activation in plant and animal receptor kinases. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Location and role of free cysteinyl residues in the Sindbis virus E1 and E2 glycoproteins

JOURNAL OF VIROLOGY, 81(12), 6231–6240.

MeSH headings : Amino Acid Sequence; Animals; Capsid / chemistry; Cell Line; Chromatography, Liquid; Cricetinae; Cysteine / chemistry; Disulfides / chemistry; Iodoacetamide / pharmacology; Mass Spectrometry; Membrane Glycoproteins / chemistry; Molecular Sequence Data; Mutation; Protein Conformation; Viral Envelope Proteins / chemistry
TL;DR: Native virus particles alkylated with iodoacetamide demonstrated a 4-log decrease in viral infectivity, which suggests that the modification of free cysteinyl residues results in the loss of infectivity by destabilizing the virus particle or that a rearrangement of disulfide bonds, which is required forinfectivity, is blocked by the modification. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Membrane proteomic analysis of Arabidopsis thaliana using alternative solubilization techniques

JOURNAL OF PROTEOME RESEARCH, 6(5), 1933–1950.

By: S. Mitra n, J. Gantt n, J. Ruby n, S. Clouse n & M. Goshe n

author keywords: arabidopsis; proteomics; liquid chromatography; mass spectrometry; membrane proteins; phosphorylation; leucine-rich repeat receptor-like kinases
MeSH headings : Amino Acid Sequence; Arabidopsis / chemistry; Arabidopsis / cytology; Arabidopsis Proteins / analysis; Arabidopsis Proteins / genetics; Arabidopsis Proteins / isolation & purification; Bacteriorhodopsins / analysis; Bacteriorhodopsins / genetics; Bacteriorhodopsins / isolation & purification; Cetomacrogol / chemistry; Chromatography, Liquid / methods; Cytochromes c / analysis; Cytochromes c / genetics; Cytochromes c / isolation & purification; Membrane Proteins / analysis; Membrane Proteins / genetics; Membrane Proteins / isolation & purification; Methanol / chemistry; Molecular Sequence Data; Peptides / analysis; Peptides / chemistry; Peptides / genetics; Proteome / analysis; Solubility; Surface-Active Agents / chemistry; Tandem Mass Spectrometry / methods
TL;DR: Overall, the data show that methanol is a better alternative for identifying a wider range of membrane proteins than the nonionic detergent Brij-58. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Tandem mass spectrometry acquisition approaches to enhance identification of protein-protein interactions using low-energy collision-induced dissociative chemical crosslinking reagents

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 21(21), 3395–3408.

By: E. Soderblom n, B. Bobay n, J. Cavanagh n & M. Goshe n

MeSH headings : Amino Acid Sequence; Bacillus subtilis / chemistry; Bacillus subtilis / metabolism; Bacterial Proteins / chemistry; Cross-Linking Reagents / chemistry; DNA-Binding Proteins / chemistry; Molecular Sequence Data; Peptide Mapping; Peptides / chemistry; Protein Conformation; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry / methods; Transcription Factors / chemistry
TL;DR: The ability to segregate intrapeptide and interpeptide crosslinks using ITCID represents the first step towards high-throughput analysis of protein-protein cross links using the CID-CXL reagents. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

A mass spectrometry-based proteomic approach to study Marek's Disease Virus gene expression

Journal of Virological Methods, 135(1), 66–75.

By: H. Liu n, E. Soderblom n & M. Goshe n

author keywords: proteomics; gene expression; liquid chromatography; mass spectrometry; Marek's Disease Virus
MeSH headings : Animals; Cells, Cultured; Chick Embryo; Chromatography, Ion Exchange; Computational Biology; Databases, Protein; Fibroblasts / virology; Gas Chromatography-Mass Spectrometry; Gene Expression Profiling / methods; Herpesvirus 2, Gallid / genetics; Proteome / analysis; Proteome / isolation & purification; Proteomics / methods; Trypsin / metabolism; Viral Proteins / analysis; Viral Proteins / isolation & purification
TL;DR: A mass spectrometry-based strategy was developed to identify viral proteins and to qualitatively examine their abundance in lytically infected chicken embryo fibroblast (CEF) cells and it is anticipated that this approach will be a viable method for determining how viral and host proteome changes occurring in Marek's Disease pathogenesis regulate the switch between the lytic and latent phases of the MDV life cycle. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref
Added: August 6, 2018

2006 journal article

Collision-induced dissociative chemical cross-linking reagents and methodology: Applications to protein structural characterization using tandem mass spectrometry analysis

ANALYTICAL CHEMISTRY, 78(23), 8059–8068.

By: E. Soderblom n & M. Goshe n

MeSH headings : Amino Acid Sequence; Animals; Aspartic Acid / chemistry; Cross-Linking Reagents / chemistry; Glutathione Transferase / chemistry; Glutathione Transferase / genetics; Glutathione Transferase / metabolism; Models, Molecular; Molecular Sequence Data; Proline / chemistry; Protein Structure, Tertiary; Recombinant Fusion Proteins / chemistry; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Schistosoma japonicum / genetics; Schistosoma japonicum / metabolism; Serum Albumin, Bovine / chemistry; Serum Albumin, Bovine / genetics; Serum Albumin, Bovine / metabolism; Tandem Mass Spectrometry / methods
TL;DR: Common acquisition approaches such as data-dependent acquisition experiments using ion trap mass spectrometers and product ion spectral analysis using SEQUEST were shown to be compatible with the CID-CXL-MS/MS reagents, obviating the requirement for high resolution and high mass accuracy measurements to identify both intra- and interpeptide cross-links. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Novel protein purification system utilizing an N-terminal fusion protein and a caspase-3 cleavable linker

Protein Expression and Purification, 47(1), 311–318.

By: B. Feeney n, E. Soderblom n, M. Goshe n & A. Clark n

author keywords: caspase-3; fusion protein; protein expression; proteolysis
MeSH headings : Amino Acid Sequence; Caspase 3 / metabolism; Caspase 3 / physiology; Glutathione Transferase / genetics; Histidine / genetics; Hydrolysis; Molecular Sequence Data; Peptide Fragments / chemistry; Peptide Fragments / genetics; Peptide Fragments / metabolism; Protein Structure, Tertiary / genetics; Proteins / genetics; Proteins / isolation & purification; Proteins / metabolism; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / isolation & purification; Recombinant Fusion Proteins / metabolism
TL;DR: A glutathione S-transferase-fusion protein vector with a caspase-3 consensus cleavage sequence located between the N-terminal GST tag and a target protein is designed and it is shown that the enzyme efficiently cleaves the fusion protein without leaving excess amino acids on the target protein. (via Semantic Scholar)
Sources: Crossref, Web Of Science
Added: August 6, 2018

2005 journal article

Identification and functional analysis of in vivo phosphorylation sites of the Arabidopsis BRASSINOSTEROID-INSENSITIVE1 receptor kinase

PLANT CELL, 17(6), 1685–1703.

MeSH headings : Arabidopsis / enzymology; Arabidopsis / genetics; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Binding Sites / physiology; Cell Membrane / metabolism; Conserved Sequence / genetics; Mutation / genetics; Phosphorylation; Protein Kinases / chemistry; Protein Kinases / genetics; Protein Kinases / metabolism; Protein Serine-Threonine Kinases / chemistry; Protein Serine-Threonine Kinases / genetics; Protein Serine-Threonine Kinases / metabolism; Protein Structure, Tertiary / physiology; Serine / chemistry; Serine / metabolism; Signal Transduction / physiology; Threonine / chemistry; Threonine / metabolism
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Evaluation of the DNA Binding Tendencies of the Transition State Regulator AbrB†

Biochemistry, 43(51), 16106–16118.

By: B. Bobay n, L. Benson n, S. Naylor n, B. Feeney n, A. Clark n, M. Goshe n, M. Strauch n, R. Thompson n, J. Cavanagh n

MeSH headings : Bacillus subtilis / metabolism; Circular Dichroism; DNA / chemistry; DNA / metabolism; Kinetics; Protein Binding / physiology; Spectrometry, Mass, Electrospray Ionization; Transcription Factors / chemistry; Transcription Factors / metabolism
TL;DR: This comprehensive and corroborative spectroscopic study endorses the use of microESI-MS for rapidly ascertaining qualitative binding trends in noncovalent systems in a high-throughput manner. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, Web Of Science
Added: August 6, 2018

2004 journal article

Global Analysis of the Membrane Subproteome of Pseudomonas aeruginosaUsing Liquid Chromatography-Tandem Mass Spectrometry

Journal of Proteome Research, 3(3), 434–444.

By: J. Blonder*, M. Goshe*, W. Xiao*, D. Camp*, M. Wingerd*, R. Davis*, R. Smith*

author keywords: proteome; membrane proteins; low abundance; LC-MS/MS; affinity labeling
MeSH headings : Affinity Labels / chemistry; Amino Acid Sequence; Chromatography, Liquid; Databases, Protein; Mass Spectrometry; Membranes / metabolism; Molecular Sequence Data; Multiprotein Complexes / metabolism; Proteome; Pseudomonas aeruginosa / metabolism
TL;DR: Key integral inner and outer membrane proteins involved in adaptation and antibiotic resistance were conclusively identified, including the detection of 53% of all predicted opr-type porins (outer integral membrane proteins) and all the components of the mexA-mexB-oprM transmembrane protein complex. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: Crossref
Added: November 22, 2020

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