Works (139)

Updated: April 4th, 2024 10:37

2022 journal article

Bioprocess intensification: A route to efficient and sustainable biocatalytic transformations for the future

CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION, 172.

By: K. Boodhoo*, M. Flickinger n, J. Woodley* & E. Emanuelsson*

author keywords: Bioprocess intensification; Biocatalyst engineering; Bioreactor; Upstream and downstream processing; In-situ product recovery; Hybrid; multifunctional technologies
TL;DR: The future prospects of bio-process intensification are promising but there are still challenges that need to be overcome to fully exploit this technology. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: May 10, 2022

2021 journal article

A concept for continuous virus manufacture using a moving bed bioreactor: Growth of MDCK cells to confluence on paper as a model support

CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION, 170.

By: C. Duffy n, L. Overton n & M. Flickinger n

author keywords: Cell culture on paper; Moving bed bioreactor; Continuous virus manufacture; Intensification of vaccine manufacture; MDCK cell growth on paper
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: November 15, 2021

2020 journal article

A falling film bioreactor (FFBR) for generating effective gas-to-liquid mass transfer using wavy laminar flow for continuous microbial gas processing

CHEMICAL ENGINEERING SCIENCE, 219.

By: R. Barton n, K. VanTreeck n, C. Duran n, M. Schulte n & M. Flickinger n

author keywords: Falling film bioreactor; Biocomposite biocatalyst; Cell immobilization; Process intensification; Gas-liquid mass transfer; Gas-to-liquid chemicals
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: May 26, 2020

2020 journal article

Loofah-based microalgae and cyanobacteria biocomposites for intensifying carbon dioxide capture

JOURNAL OF CO2 UTILIZATION, 42.

author keywords: Algae oil; Biocoating; Bioenergy with carbon capture and storage (BECCS); Carbon capture and storage; Intensified carbon capture and utilisation; Latex immobilisation
TL;DR: Analysis of the kinetics of CO2 absorbtion and SEM imaging suggests that the cells were embedded within a polymer film that covered the scaffold, and shows promise to intensify biological CO2 capture and possible application in bioenergy with carbon capture and storage (BECCS). (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: January 11, 2021

2019 journal article

A Microalgae Biocomposite-Integrated Spinning Disk Bioreactor (SDBR): Toward a Scalable Engineering Approach for Bioprocess Intensification in Light-Driven CO2 Absorption Applications

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 58(15), 5936–5949.

By: T. Ekins-Coward*, K. Boodhoo*, S. Velasquez-Orta*, G. Caldwell*, A. Wallace n, R. Barton n, M. Flickinger n

TL;DR: Overall, the increased CO2 biofixation with a greatly reduced biocomposite surface area and the high cell retention in this proof-of-concept technology highlight the bioprocess intensificatio... (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: May 13, 2019

2019 journal article

Experiments and finite element modeling of hydrodynamics and mass transfer for continuous gas-to-liquid biocatalysis using a biocomposite falling film reactor

CHEMICAL ENGINEERING SCIENCE, 209.

By: M. Schulte n, M. Robinett n, N. Weidle n, C. Duran n & M. Flickinger n

author keywords: Falling film; Computational fluid dynamics; Gas-to-liquid mass transfer; Biocomposite biocatalyst; Process intensification; Immobilized cells in biocomposites
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: December 30, 2019

2017 journal article

A technique for lyopreservation of Clostridium ljungdahlii in a biocomposite matrix for CO absorption

PLOS ONE, 12(7).

By: M. Schulte n, J. Solocinski*, M. Wang*, M. Kovacs n, R. Kilgore n, Q. Osgood*, L. Underwood*, M. Flickinger n, N. Chakraborty*

MeSH headings : Biocompatible Materials; Carbon Monoxide / metabolism; Clostridium / metabolism; Preservation, Biological / methods; Spectrum Analysis, Raman / methods
TL;DR: The present study successfully extends and implements the principles of dry-stabilization for preservation of strictly anaerobic bacteria as an alternative to lyophilization or spray drying that could enable centralized biocomposite biocatalyst fabrication and decentralized bioprocessing of CO to liquid fuels or chemicals. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 review

Biocoatings: A new challenge for environmental biotechnology

[Review of ]. BIOCHEMICAL ENGINEERING JOURNAL, 121, 25–37.

By: S. Cortez*, A. Nicolau*, M. Flickinger n & M. Mota

author keywords: Biocatalysis; Environmental biotechnology; Nanoporous latex coating; Non-growing cells
TL;DR: This review highlights recent advances in biocoating technology for pollutants degradation, photo-reactive coatings, stabilization of hyperthermophiles for biocatalysis, environmental biosensors, and biocomposite fuel cells. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 article

Biocoatings: challenges to expanding the functionality of waterborne latex coatings by incorporating concentrated living microorganisms

Flickinger, M. C., Bernal, O. I., Schulte, M. J., Broglie, J. J., Duran, C. J., Wallace, A., … Velev, O. D. (2017, July). JOURNAL OF COATINGS TECHNOLOGY AND RESEARCH, Vol. 14, pp. 791–808.

By: M. Flickinger n, O. Bernal n, M. Schulte n, J. Broglie n, C. Duran n, A. Wallace n, C. Mooney n, O. Velev n

author keywords: Biocoatings; Biocatalysts; Waterborne coating functionality; Nanoporous coatings; Engineered microbes in coatings; Bioabsorbers; Desiccation tolerance
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

Dynamic adsorption of ammonia: apparatus, testing conditions, and adsorption capacities

MEASUREMENT SCIENCE AND TECHNOLOGY, 28(5).

By: H. Amid n, B. Maze n, M. Flickinger n & B. Pourdeyhimi n

author keywords: Adsorption capacity/breakthrough; ammonia; activated carbon; test methods; gas detector
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

Fabrication of Photoreactive Biocomposite Coatings via Electric Field-Assisted Assembly of Cyanobacteria

Langmuir, 33(21), 5304–5313.

By: O. Bernal, B. Bharti*, M. Flickinger* & O. Velev*

MeSH headings : Biocompatible Materials; Biomimetics; Cell Survival; Cyanobacteria; Polyesters
TL;DR: The assembly of structured cyanobacteria coatings that optimize cell surface coverage and preserve cell viability after immobilization are achieved, a step toward the development of flexible multilayered cell-based photoabsorbing biomaterials that can serve as components of "biomimetic leaves" for utilizing solar energy to recycle CO2 into fuels or chemicals. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Crossref, NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2017 journal article

Microbial Paper: Cellulose Fiber-based Photo-Absorber Producing Hydrogen Gas from Acetate using Dry-Stabilized Rhodopseudomonas palustris

BIORESOURCES, 12(2), 4013–4030.

By: O. Bernal n, J. Pawlak n & M. Flickinger n

author keywords: Cellular biocomposite; Microbial paper; Microfibrillar cellulose; Cellular immobilization
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

A High Gas Fraction, Reduced Power, Syngas Bioprocessing Method Demonstrated With a Clostridium ljungdahlii OTA1 Paper Biocomposite

BIOTECHNOLOGY AND BIOENGINEERING, 113(9), 1913–1923.

By: M. Schulte n, J. Wiltgen n, J. Ritter n, C. Mooney n & M. Flickinger n

author keywords: Clostridium ljungdahlii; gas-to-liquid fuels; biocomposite; process intensification; biofuels; syngas
MeSH headings : Acetates / metabolism; Biofuels; Bioreactors / microbiology; Clostridium / metabolism; Ethanol / metabolism; Gases / metabolism; Paper
TL;DR: A miniaturized, coated‐paper strip, high gas fraction, biocomposite absorber has been developed using slowly shaken horizontal anaerobic tubes to demonstrate elevated CO mass transfer with low power input, reduced liquid requirements, elevated substrate consumption, and increased product secretion compared to shaken suspended cells. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

Characterization of Clostridium ljungdahlii OTA1: a non-autotrophic hyper ethanol-producing strain

Applied Microbiology and Biotechnology, 101(4), 1615–1630.

By: J. Whitham n, M. Schulte n, B. Bobay*, J. Bruno-Barcena n, M. Chinn n, M. Flickinger n, J. Pawlak n, A. Grunden n

Contributors: J. Whitham n, M. Schulte n, B. Bobay*, J. Bruno-Barcena n, M. Chinn n, M. Flickinger n, J. Pawlak n, A. Grunden n

author keywords: Acetyl-coA; Carbon monoxide dehydrogenase; Clostridium ljungdahlii; Ethanol; Wood-Ljungdahl pathway
MeSH headings : Acetyl Coenzyme A / metabolism; Aldehyde Oxidoreductases / metabolism; Carbon Dioxide / metabolism; Carbon Monoxide / metabolism; Clostridium / metabolism; Ethanol / metabolism; Multienzyme Complexes / metabolism
TL;DR: A non-autotrophic hyper ethanol-producing strain of C. ljungdahlii was identified that has utility for further physiology and strain performance studies and as a biocatalyst for industrial applications. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2016 review

Hybrid adsorbent nonwoven structures: a review of current technologies

[Review of ]. JOURNAL OF MATERIALS SCIENCE, 51(9), 4173–4200.

By: H. Amid n, B. Maze n, M. Flickinger n & B. Pourdeyhimi n

UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Growth of Bacillus methanolicus in 2 M methanol at 50 A degrees C: the effect of high methanol concentration on gene regulation of enzymes involved in formaldehyde detoxification by the ribulose monophosphate pathway

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 42(7), 1027–1038.

By: A. Bozdag n, C. Komives* & M. Flickinger n

author keywords: Methylotrophy; Bacillus methanolicus; Formaldehyde tolerance; Formaldehyde toxicity; RUMP pathway
MeSH headings : Alcohol Oxidoreductases / genetics; Alcohol Oxidoreductases / metabolism; Bacillus / enzymology; Bacillus / genetics; Bacillus / growth & development; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Culture Media; Formaldehyde / metabolism; Gene Expression; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Glucose / metabolism; Mannitol / metabolism; Metabolic Networks and Pathways; Methanol / metabolism; Ribulosephosphates / metabolism
TL;DR: It is shown that methanol oxidation in B. methanolicus MGA3 is mainly carried out by the pBM19-encoded mdh, which suggests that pfk may be a possible synthetic methylotrophy target to reduce formaldehyde growth toxicity at high meethanol concentrations. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Biocatalytic Coatings for Air Pollution Control: A Proof of Concept Study on VOC Biodegradation

BIOTECHNOLOGY AND BIOENGINEERING, 112(2), 263–271.

By: J. Estrada*, O. Bernal n, M. Flickinger n, R. Munoz* & M. Deshusses*

author keywords: air pollution; bioactive latex coating; biological paint; Pseudomonas putida; toluene; VOC; biofiltration
MeSH headings : Air Pollutants / analysis; Air Pollutants / chemistry; Air Pollutants / isolation & purification; Biodegradation, Environmental; Biofilms; Pseudomonas putida; Volatile Organic Compounds / analysis; Volatile Organic Compounds / chemistry; Volatile Organic Compounds / isolation & purification
TL;DR: This study constitutes the first application of bioactive latex coatings for VOC abatement and could become promising means for air pollution control. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Specific Photosynthetic Rate Enhancement by Cyanobacteria Coated Onto Paper Enables Engineering of Highly Reactive Cellular Biocomposite "Leaves"

BIOTECHNOLOGY AND BIOENGINEERING, 111(10), 1993–2008.

By: O. Bernal n, C. Mooney n & M. Flickinger n

author keywords: cellular biocomposites; gas-phase biocatalysis; cyanobacteria specific photosynthetic rates; biomimetic leaf
MeSH headings : Anabaena / growth & development; Anabaena / physiology; Biomimetic Materials / metabolism; Biomimetics / methods; Bioreactors; Carbon Dioxide / metabolism; Cell Engineering / methods; Cells, Immobilized / physiology; Nitrates / metabolism; Oxygen / metabolism; Paper; Photosynthesis; Synechococcus / growth & development; Synechococcus / physiology; Synechocystis / growth & development; Synechocystis / physiology
TL;DR: This is the first report of changes of cyanobacteria tolerance to temperature and light intensities after deposition as a thin coating on a porous matrix, which has important implications for gas‐phase photobioreactor design using porous composite materials. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 book

Encyclopedia of industrial biotechnology. Selections

Hoboken, New Jersey: Wiley.

Michael Flickinger

Source: NC State University Libraries
Added: August 6, 2018

2013 review

Engineering Cellular Photocomposite Materials Using Convective Assembly

[Review of ]. MATERIALS, 6(5), 1803–1825.

By: J. Jenkins n, M. Flickinger n & O. Velev n

Contributors: J. Jenkins n, M. Flickinger n & O. Velev n

author keywords: cellular photocomposites; continuous convective-sedimentation assembly; colloidal coatings; artificial leaves; biocomposite materials
TL;DR: This paper reviews the non-biological convective assembly literature, with an emphasis on how the method can be modified to deposit living cells starting from a batch process to its current state as a continuous process capable of fabricating larger multi-layer biocomposite coatings from diverse particle suspensions. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2013 journal article

Preservation of H-2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures

MICROBIAL BIOTECHNOLOGY, 6(5), 515–525.

By: M. Piskorska*, T. Soule*, J. Gosse n, C. Milliken*, M. Flickinger n, G. Smith*, C. Yeager*

MeSH headings : Bioreactors / microbiology; Biotechnology / methods; Cells, Immobilized / metabolism; Desiccation; Hydrogen / metabolism; Latex; Rhodopseudomonas / drug effects; Rhodopseudomonas / metabolism; Rhodopseudomonas / radiation effects; Temperature
TL;DR: The results demonstrate that biocatalytic latex coatings are an attractive cell immobilization platform for preservation of bioactivity in the dry state, implicating oxidative damage as a key factor limiting coating storage. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 book

Upstream industrial biotechnology

Hoboken, New Jersey: John Wiley & Sons Inc.

Michael Flickinger

Source: NC State University Libraries
Added: August 6, 2018

2012 journal article

A versatile method for preparation of hydrated microbial–latex biocatalytic coatings for gas absorption and gas evolution

Journal of Industrial Microbiology & Biotechnology, 39(9), 1269–1278.

By: J. Gosse n, M. Chinn n, A. Grunden n, O. Bernal n, J. Jenkins n, C. Yeager*, S. Kosourov*, M. Seibert*, M. Flickinger n

author keywords: Latex coating immobilization on chromatography paper; Chlamydomonas; Rhodopseudomonas; Clostridium; Synechococcus
MeSH headings : Absorption; Bacterial Adhesion; Biocatalysis; Bioreactors; Carbon Dioxide / metabolism; Carbon Monoxide / metabolism; Gases / chemistry; Gases / metabolism; Hydrogen / metabolism; Latex / chemistry; Oxygen / metabolism; Paper; Rhodopseudomonas / growth & development; Rhodopseudomonas / metabolism
TL;DR: The immobilization of different microorganisms in thin adhesive films in the gas phase demonstrates the utility of this method for evaluating genetically optimized microorganisms for gas absorption and gas evolution. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2012 journal article

Deposition of composite coatings from particle–particle and particle–yeast blends by convective-sedimentation assembly

Journal of Colloid and Interface Science, 380(1), 192–200.

By: J. Jenkins n, M. Flickinger n & O. Velev n

Contributors: J. Jenkins n, M. Flickinger n & O. Velev n

author keywords: Colloidal coatings; Size-selective segregation; Bimodal blends; Convective assembly of biocomposite yeast blends
MeSH headings : Aluminum / chemistry; Cells, Immobilized / cytology; Colloids / chemistry; Glass / chemistry; Microspheres; Particle Size; Polyesters / chemistry; Polypropylenes / chemistry; Saccharomyces cerevisiae / cytology; Sulfates / chemistry
TL;DR: This study shows how substrate wettability, suspension composition, particle size ratio and surface charge affect the deposition process and resulting coating microstructure (particle ordering and void space) and confirms the existence of significant polymer particle-yeast interactions and formation of particle aggregates that disrupt coatingmicrostructure during deposition. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID, NC State University Libraries
Added: August 6, 2018

2012 journal article

Modeling the influence of slurry concentration on Saccharomyces cerevisiae cake porosity and resistance during microfiltration

BIOTECHNOLOGY PROGRESS, 28(6), 1534–1541.

By: M. Mota & M. Flickinger n

author keywords: microfiltration; cake resistance; concentration; yeast; porosity
MeSH headings : Biotechnology / methods; Cell Culture Techniques / methods; Computer Simulation; Filtration / methods; Porosity; Pressure; Saccharomyces cerevisiae / chemistry; Suspensions
TL;DR: In the range of moderate yeast concentration, the filtrate flux may be increased by manipulating the filtration pressure and the slurry concentration, thereby improving the overall process efficiency. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Engineering and modeling of thin, adhesive, microbial biocatalytic coatings for high intensity oxidations in multi-phase microchannel bioreactors

Chemical Engineering Science, 66(14), 3251–3257.

By: M. Fidaleo* & M. Flickinger n

author keywords: Biochemical engineering; Bioreactors; Immobilization of Gluconobacter oxydans; Microchannel bioreactors; Mathematical modeling; Multi-phase reactors
TL;DR: These modeling studies should provide the basis for engineering of channel geometry, biocatalytic coating nano-porosity and thickness, coating stability, optimal reactivity and multi-phase channel flow properties for future microchannel bioreactors for high intensity microbial oxidations. (via Semantic Scholar)
UN Sustainable Development Goal Categories
1. No Poverty (Web of Science)
Source: Crossref
Added: February 24, 2020

2010 patent

A Structured Microbial Material for the Production of Hydrogen

Washington, DC: U.S. Patent and Trademark Office.

By: M. Flickinger, F. Rey & C. Harwood

Source: NC State University Libraries
Added: August 6, 2018

2010 journal article

Bacillus methanolicus pyruvate carboxylase and homoserine dehydrogenase I and II and their roles for l-lysine production from methanol at 50°C

Applied Microbiology and Biotechnology, 87(3), 951–964.

By: T. Brautaset*, Ø. Jakobsen*, K. Degnes*, R. Netzer*, I. Nærdal*, A. Krog*, R. Dillingham*, M. Flickinger n, T. Ellingsen*

author keywords: Thermotolerant; Methylotrophy; Quantitative PCR; Anaplerotic; Fed batch fermentation; Metabolic engineering
MeSH headings : Bacillus / enzymology; Bacillus / genetics; Bacillus / metabolism; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Cloning, Molecular; Fermentation; Glutamic Acid / metabolism; Homoserine Dehydrogenase / genetics; Homoserine Dehydrogenase / metabolism; Hot Temperature; Lysine / biosynthesis; Methanol / metabolism; Methionine / metabolism; Molecular Sequence Data; Mutation; Pyruvate Carboxylase / genetics; Pyruvate Carboxylase / metabolism; Threonine / metabolism
TL;DR: It is demonstrated that hom-1 represents one key target for achieving l-lysine overproduction, PC activity plays an important role in controlling l-glutamate production from methanol, and that OAA precursor supply is not a major bottleneck for l- Elysian overproduction by B. methanolicus. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2010 journal article

Progress toward a biomimetic leaf: 4,000 h of hydrogen production by coating-stabilized nongrowing photosyntheticRhodopseudomonas palustris

Biotechnology Progress, NA-NA.

By: J. Gosse*, B. Engel*, J. Hui*, C. Harwood* & M. Flickinger*

author keywords: biomimetic leaf; solar energy; thin-film photobioreactor; hydrogen production; Rhodopseudomonas palustris; microbial photosynthesis; nongrowing
MeSH headings : Biomimetics / methods; Bioreactors / microbiology; Hydrogen / metabolism; Photochemistry / methods; Photosynthesis / physiology; Rhodopseudomonas / metabolism
TL;DR: A combined materials and modeling approach could be used for guiding cellular engineering of light trapping and reactivity to enhance overall photosynthetic efficiency per meter square of sunlight incident on photocatalysts. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries
Added: January 24, 2021

2009 journal article

Thermodynamic Feasibility of Enzymatic Reduction of Carbon Dioxide to Methanol

Applied Biochemistry and Biotechnology, 162(2), 391–398.

By: F. Baskaya*, X. Zhao*, M. Flickinger n & P. Wang*

author keywords: Carbon dioxide; Enzymatic biocatalysis; Reduction; Sequestration; Methanol; Thermodynamics
MeSH headings : Biocatalysis; Carbon Dioxide / metabolism; Feasibility Studies; Hydrogen-Ion Concentration; Methanol / metabolism; Osmolar Concentration; Oxidation-Reduction; Thermodynamics
TL;DR: The analysis showed that the enzymatic conversion of carbon dioxide is highly sensitive to the pH value of the reaction solution and, by conducting the reactions at low pHs and ionic strength, it is possible to shift the biological methanol metabolic reaction equilibrium constants significantly to favor the synthesis of meethanol. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Crossref
Added: January 21, 2021

2008 journal article

Spatial expression of a mercury-inducible green fluorescent protein within a nanoporous latex-based biosensor coating

Journal of Industrial Microbiology & Biotechnology, 35(4), 283–290.

By: J. Schottel*, P. Orwin*, C. Anderson* & M. Flickinger n

author keywords: mercury; biosensor; nanoporous latex; laser scanning confocal microscopy; GFP
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Biosensing Techniques / methods; Cells, Immobilized / metabolism; Escherichia coli / genetics; Escherichia coli / metabolism; Gene Expression; Genes, Reporter; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Latex / chemistry; Mercury / metabolism; Microscopy, Confocal; Temperature; Time Factors
TL;DR: To quantify and localize gene expression within a latex-based mercury biosensor, a plasmid, pmerGFP, was constructed, which contains the green fluorescent protein (GFP) gene under transcriptional control of the mercury resistance operon regulatory sequences. (via Semantic Scholar)
Source: Crossref
Added: February 24, 2020

2007 journal article

Bacillus methanolicus: a candidate for industrial production of amino acids from methanol at 50°C

Applied Microbiology and Biotechnology, 74(1), 22–34.

By: T. Brautaset*, Ø. Jakobsen*, K. Josefsen*, M. Flickinger* & T. Ellingsen*

MeSH headings : Bacillus / genetics; Bacillus / growth & development; Bacillus / metabolism; Bacterial Proteins / genetics; Biotechnology / methods; Genetic Engineering; Glutamates / biosynthesis; Lysine / biosynthesis; Methanol / metabolism; Plasmids / genetics; Temperature
TL;DR: This mini review highlights the unique genetics and favorable physiological traits of thermotolerant methylotroph Bacillus methanolicus, which makes it an interesting candidate for overproduction of amino acids from methanol. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2007 journal article

Electrochemical characterization ofGeobacter sulfurreducens cells immobilized on graphite paper electrodes

Biotechnology and Bioengineering, 99(5), 1065–1073.

By: S. Srikanth*, E. Marsili*, M. Flickinger* & D. Bond*

author keywords: Geobacter; microbial fuel cell; electrochemistry
MeSH headings : Electrochemistry; Electrodes; Ferric Compounds / metabolism; Geobacter / metabolism; Graphite; Industrial Microbiology / methods; Oxidation-Reduction; Paper; Pectins
TL;DR: The ability of washed G. sulfurreducens cells to immediately produce electrical current was consistent with the external surface of this bacterium possessing a pathway linking oxidative metabolism to extracellular electron transfer, and illustrates a strategy for preparation of catalytic electrodes and study of Geobacter under defined conditions. (via Semantic Scholar)
Source: Crossref
Added: February 24, 2020

2007 journal article

Modelling diffusivity in porous polymeric membranes with an intermediate layer containing microbial cells

Biochemical Engineering Journal, 37(3), 285–293.

By: M. Mota, A. Yelshin*, M. Fidaleo* & M. Flickinger*

author keywords: diffusion; microporous membrane; immobilised cells; modelling; packing; mass transfer
TL;DR: It was found that the layered system is sensitive to the latex coatings porosity when the composite layer occupies less than 50% of the total membrane system thickness. (via Semantic Scholar)
Source: Crossref
Added: February 24, 2020

2007 journal article

Painting and Printing Living Bacteria: Engineering Nanoporous Biocatalytic Coatings to Preserve Microbial Viability and Intensify Reactivity

Biotechnology Progress, 23(1), 2–17.

By: M. Flickinger*, J. Schottel*, D. Bond*, A. Aksan* & L. Scriven*

MeSH headings : Bacterial Physiological Phenomena; Catalysis; Cell Culture Techniques / methods; Cell Proliferation; Cell Survival; Coated Materials, Biocompatible / chemistry; Microfluidics / methods; Nanostructures / chemistry; Nanostructures / ultrastructure; Tissue Engineering / methods
TL;DR: Expanded use of nanoporous adhesive coatings for prokaryotic and eukaryotic cell preservation at ambient temperature and the design of highly reactive “living” paints and inks is anticipated. (via Semantic Scholar)
Source: Crossref
Added: February 24, 2020

2006 journal article

68. Quantification of the desiccation response of Geobacter sulfurreducens at the molecular level

Cryobiology, 53(3), 396–397.

By: V. Ragoonanan*, S. Srikanth*, W. Wolkers*, D. Bond*, M. Flickinger* & A. Aksan*

Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2006 journal article

A model system for increasing the intensity of whole-cell biocatalysis: Investigation of the rate of oxidation ofD-sorbitol toL-sorbose by thin bi-layer latex coatings of non-growingGluconobacter oxydans

Biotechnology and Bioengineering, 95(3), 446–458.

By: M. Fidaleo*, S. Charaniya*, C. Solheid*, U. Diel*, M. Laudon*, H. Ge*, L. Scriven*, M. Flickinger*

author keywords: bioprocess intensity; oxidation of D-sorbitol to L-sorbose; immobilization of Gluconobacter oxydans; biocatalytic latex coating; effectiveness factor/intrinsic kinetics; diffusion-reaction model
MeSH headings : Bioreactors / microbiology; Catalysis; Cells, Immobilized / metabolism; Gluconobacter oxydans / metabolism; Industrial Microbiology / methods; Kinetics; Microbial Viability; Microspheres; Nanostructures; Oxidation-Reduction; Sorbitol / metabolism; Temperature
TL;DR: These nano‐structured coatings and the possibility of improving their ability to preserve G. oxydans viability may be useful for engineering highly reactive adhesive coatings for multi‐phase micro‐channel and membrane bioreactors to dramatically increase the intensity of whole‐cell oxidations. (via Semantic Scholar)
Source: Crossref
Added: January 21, 2021

2006 patent

Composite Devices Incorporating Biological Material and Methods

Washington, DC: U.S. Patent and Trademark Office.

By: O. Lyngberg, M. Flickinger, L. Scriven & C. Anderson

Source: NC State University Libraries
Added: August 6, 2018

2006 journal article

Effect of carbohydrates on biothermodynamic properties of bacteria

Journal of Biomechanics, 39, S380.

By: V. Ragoonanan, S. Srikanth, W. Wolkers, D. Bond, M. Flickinger* & A. Aksan

Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2005 journal article

Growth of Bacillus methanolicus in seawater-based media

Journal of Industrial Microbiology & Biotechnology, 32(2), 61–66.

By: C. Komives*, L. Cheung*, S. Pluschkell* & M. Flickinger*

author keywords: Bacillus methanolicus; salt-tolerant; seawater; adaptation
TL;DR: The growth of strain PB1 in shake flask experiments with trypticase soy broth medium showed minimal salt-inhibition at the concentration of NaCl in seawater, and the ability of B. methanolicus PB1 to grow in Pacific Ocean water using methanol as a carbon and energy source was tested. (via Semantic Scholar)
Source: Crossref
Added: February 24, 2020

2005 journal article

Permeability and reactivity of Thermotoga maritima in latex bimodal blend coatings at 80°C: a model high temperature biocatalytic coating

Extremophiles, 9(3), 197–207.

By: O. Lyngberg*, C. Solheid*, S. Charaniya*, Y. Ma*, V. Thiagarajan*, L. Scriven*, M. Flickinger*

author keywords: entrapped Thermotoga maritima; latex coatings; bimodal polymer blends; latex permeability; thermostable biocatalytic coatings
MeSH headings : Biofilms; Catalysis; Cryoelectron Microscopy; Ecosystem; Hot Temperature; Latex; Permeability; Thermotoga maritima / growth & development; Thermotoga maritima / physiology; Thermotoga maritima / ultrastructure
TL;DR: Coating permeability (pore structure) was critical for maintaining T. maritima viability and the observed reactivity and stability of these coatings was surprising since cryo-SEM images suggested that the smaller low Tg polyacrylate/styrene particles preferentially bound to the T. Maritima toga-sheath during coat formation. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2003 patent

Genes Encoding Resistance to DNA Bioreductive Alkylation of Cleaving Agents and Method for Identification of Compounds that Inhibit Resistance to DNA Bioreductive Alkylating and Cleaving Agents

Washington, DC: U.S. Patent and Trademark Office.

By: D. Sherman, P. August & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

2001 patent

Production of Amino Acids using Auxotrophic Mutants of Methylotrophic Bacillus

Washington, DC: U.S. Patent and Trademark Office.

By: R. Hanson, M. Flickinger, F. Schendel & M. Guettler

Source: NC State University Libraries
Added: August 6, 2018

2000 patent

Production of Amino Acids using Auxotrophic Mutants of Methylotrophic Bacillus

Washington, DC: U.S. Patent and Trademark Office.

By: R. Hanson, M. Flickinger, P. Olson, W. Hur, N. Al-Tahoo & C. Bremmon

Source: NC State University Libraries
Added: August 6, 2018

2000 patent

Protein Adsorption by Very Dense Porous Zirconium Oxide Particles in Expanded Beds

Washington, DC: U.S. Patent and Trademark Office.

By: M. Flickinger, C. Griffith, M. Robichaud, J. Morris, M. Annen, C. Dunlop, A. McCormick, P. Carr

Source: NC State University Libraries
Added: August 6, 2018

1999 patent

Production of Amino Acids by Methylotrophic Bacillus

Canadian Patent.

By: R. Hanson, M. Flickinger, F. Schendel & M. Guettler

Source: NC State University Libraries
Added: August 6, 2018

1998 patent

Protein Adsorption by Very Dense Porous Zirconium Oxide Particles in Expanded Beds

Washington, DC: U.S. Patent and Trademark Office.

By: M. Flickinger, C. Griffith, M. Robichaud, J. Morris, M. Annen, C. Dunlop, A. McCormick, P. Carr

Source: NC State University Libraries
Added: August 6, 2018

1997 journal article

Bacterial expression and characterization of an anti-desipramine single-chain antibody fragment

International Journal of Immunopharmacology, 19(12), 729–738.

By: G. Lin, P. Pentel, W. Shelver, D. Keyler, C. Ross, Y. Hieda, M. Flickinger, C. Pennell, M. Murtaugh

Source: NC State University Libraries
Added: August 6, 2018

1997 journal article

Fluidization characteristics of and protein adsorption on fluoride-modified porous zirconium oxide particles

JOURNAL OF CHROMATOGRAPHY A, 776(2), 179–195.

By: C. Griffith*, J. Morris*, M. Robichaud*, M. Annen*, A. McCormick* & M. Flickinger*

author keywords: zirconia; fluoride-modified zirconia; adsorbents; stationary phases, LC; proteins; albumin
MeSH headings : Adsorption; Animals; Cattle; Fluorides / chemistry; Nitrites / chemistry; Nitrites / metabolism; Protein Binding; Proteins / chemistry; Serum Albumin / chemistry; Serum Albumin / metabolism; Surface Properties; Zirconium / chemistry
TL;DR: Porous zirconia particles of specific gravity, mean particle sizes of approximately 50 microns, and terminal settling velocity of approximately 2.8 mm/s in water, were synthesized using an oil emulsion method from 1000 A colloids and were evaluated for their potential use in expanded bed protein adsorption. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1997 article

Models for polybutadiene pore wall coatings in porous zirconia

Reeder, D. H., Li, J. W., Carr, P. W., Flickinger, M. C., & McCormick, A. V. (1997, January 24). JOURNAL OF CHROMATOGRAPHY A, Vol. 760, pp. 71–79.

By: D. Reeder*, J. Li*, P. Carr*, M. Flickinger* & A. McCormick*

author keywords: zirconia; polybutadiene coatings; coating; stationary phases, LC
MeSH headings : Butadienes / chemistry; Elastomers; Models, Chemical; Osmolar Concentration; Polymers / chemistry; Porosity; Silicon Dioxide / chemistry; Surface Properties; Zirconium / chemistry
TL;DR: Three models of the changes in measured pore size distribution for cylindrical pores when a polymer is deposited in the pores by evaporation from a volatile solvent are presented to help interpret experimental nitrogen adsorption data for polybutadiene (PBD) coatings on porous zirconia. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

1996 journal article

Cryo-electron microscopy of polymer particles in a high cell density synthetic biofilm

Progress in Biotechnology, 11(Immobilized Cells), 298–303.

By: V. Thiagarajan, Y. Ming, L. Scriven & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

1996 patent

Production of Amino Acids Using Methylotrophic Bacillus

German National Patent.

By: R. Hanson, M. Flickinger, F. Schendel & M. Guettler

Source: NC State University Libraries
Added: August 6, 2018

1995 patent

Bacillus MGA3 Diaminopimelate Decarboxylase

Washington, DC: U.S. Patent and Trademark Office.

By: D. Mills & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

1995 patent

Production of Amino Acids Using Methylotrophic Bacillus

European Patent.

By: R. Hanson, M. Flickinger, F. Schendel & M. Guettler

Source: NC State University Libraries
Added: August 6, 2018

1994 patent

Direct Calcium Magnesium Acetate Production

Washington, DC: U.S. Patent and Trademark Office.

By: M. Flickinger, R. Hanson, F. Schendel, C. Anderson & P. August

Source: NC State University Libraries
Added: August 6, 2018

1993 patent

Bacillus MGA3 Aspartokinase II

Washington, DC: U.S. Patent and Trademark Office.

By: F. Schendel & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

1993 patent

Production of Amino Acids by Methylotrophic Bacillus

Australia.

By: R. Hanson, M. Flickinger, F. Schendel & M. Guettler

Source: NC State University Libraries
Added: August 6, 2018

1985 patent

Production of Ethanol by Yeasts Using Xylulose

Canada.

By: C. Gong, L. Chen, M. Flickinger & G. Tsao

Source: NC State University Libraries
Added: August 6, 2018

1984 patent

Production of Ethanol by Yeasts Using Xylulose

Washington, DC: U.S. Patent and Trademark Office.

By: C. Gong, L. Chen, M. Flickinger & G. Tsao

Source: NC State University Libraries
Added: August 6, 2018

1981 patent

Production of Ethanol by Yeasts Using Xylulose

Republic of South Africa.

By: C. Gong, L. Chen, M. Flickinger & G. Tsao

Source: NC State University Libraries
Added: August 6, 2018

1980 journal article

The effect of oxygen-enriched aeration on neomycin production by Streptomyces fradiae

Journal of Applied Biochemistry, 2(4), 280–291.

By: M. Flickinger & D. Perlman

Source: NC State University Libraries
Added: August 6, 2018

1975 journal article

MICROBIAL DEGRADATION OF ERYTHROMYCINS A AND B

JOURNAL OF ANTIBIOTICS, 28(4), 307–311.

By: M. Flickinger* & D. Perlman*

MeSH headings : Anti-Bacterial Agents / metabolism; Biodegradation, Environmental; Biological Assay; Chromatography, Thin Layer; Erythromycin / analysis; Erythromycin / metabolism; Freeze Drying; Pseudomonas / isolation & purification; Pseudomonas / metabolism; Sarcina / drug effects; Staphylococcus / drug effects
TL;DR: Growing cultures, as well as broken and lyophilized cells of pseudomonas 56 were found to degrade erythromycin A, and lyophile cells inactivated erystromycins A and B, and the enzyme system involved was constitutive. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

journal article

A cybernetic view of microbial growth: modeling of cells as optimal strategists

Dhurjati, P., Ramkrishna, D., Flickinger, M. C., & Tsao, G. T. Biotechnology and Bioengineering, 27(1), 1–9.

By: P. Dhurjati, D. Ramkrishna, M. Flickinger & G. Tsao

Source: NC State University Libraries
Added: August 6, 2018

journal article

A model of interorganelle monoclonal antibody transport and secretion in mouse hybridoma cells

Bibila, T. A., & Flickinger, M. C. Biotechnology and Bioengineering, 38(7), 767–780.

By: T. Bibila & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

A model system for increasing the intensity of whole-cell biocatalysis: investigation of the rate of oxidation of D-sorbitol to L-sorbose by thin bi-layer latex coatings of non-growing Gluconobacter oxydans.

Fidaleo, M., Charaniya, S., Solheid, C., Diel, U., Laudon, M., Ge, H., … Flickinger, M. C. Biotechnology and Bioengineering, 95(3), 446–448.

By: M. Fidaleo, S. Charaniya, C. Solheid, U. Diel, M. Laudon, H. Ge, L. Scriven, M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

A patch coating method for preparing biocatalytic films of Escherichia coli

Lyngberg, O. K., Thiagarajan, V., Stemke, D. J., Schottel, J. L., Scriven, L. E., & Flickinger, M. C. Biotechnology and Bioengineering, 62(1), 44–45.

By: O. Lyngberg, V. Thiagarajan, D. Stemke, J. Schottel, L. Scriven & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

A simple hobby computer-based off-gas analysis system

Forrest, E. H., Jansen, N. B., Flickinger, M. C., & Tsao, G. T. Biotechnology and Bioengineering, 23(2), 455–460.

By: E. Forrest, N. Jansen, M. Flickinger & G. Tsao

Source: NC State University Libraries
Added: August 6, 2018

journal article

A single-use luciferase-based mercury biosensor using Escherichia coli HB101 immobilized in a latex copolymer film

Lyngberg, O. K., Stemke, D. J., Schottel, J. L., & Flickinger, M. C. Journal of Industrial Microbiology & Biotechnology, 23(1), 668–676.

By: O. Lyngberg, D. Stemke, J. Schottel & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

A structured model for monoclonal antibody synthesis in exponentially growing and stationary phase hybridoma cells

Bibila, T., & Flickinger, M. C. Biotechnology and Bioengineering, 37(3), 210–226.

By: T. Bibila & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Accurate kinetic modeling of alkaline phosphatase in the escherichia coli periplasm: Implications for enzyme properties and substrate diffusion

Martinez, M. B., Flickinger, M. C., & Nelsestuen, G. L. Biochemistry, 35(4), 1179–1186.

By: M. Martinez, M. Flickinger & G. Nelsestuen

Source: NC State University Libraries
Added: August 6, 2018

journal article

Activation and regeneration of whole cell biocatalysts: initial and periodic induction behavior in starved Escherichia coli after immobilization in thin synthetic films

Swope, K. L., & Flickinger, M. C. Biotechnology and Bioengineering, 51(3), 360–370.

By: K. Swope & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Alteration of hybridoma viability and antibody secretion in transfectomas with inducible overexpression of protein disulfide isomerase

Kitchin, K., & Flickinger, M. C. Biotechnology Progress, 11(5), 565–574.

By: K. Kitchin & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

An approach to hierarchically structured porous zirconia aggregates

Reeder, D. H., Clausen, A. M., Annen, M. J., Carr, P. W., Flickinger, M. C., & McCormick, A. V. Journal of Colloid and Interface Science, 184(1), 328–330.

By: D. Reeder, A. Clausen, M. Annen, P. Carr, M. Flickinger & A. McCormick

Source: NC State University Libraries
Added: August 6, 2018

journal article

An enzyme immunoassay for the detection of staphylococcal protein A in affinity-purified products

Dertzbaugh, M. T., Flickinger, M. C., & Lebherz, W. B., III. Journal of Immunological Methods, 83(1), 167–177.

By: M. Dertzbaugh, M. Flickinger & W. Lebherz

Source: NC State University Libraries
Added: August 6, 2018

journal article

An improved oil emulsion synthesis method for large, porous zirconia particles for packed- or fluidized-bed protein chromatography

Robichaud, M. J., Sathyagal, A. N., Carr, P. W., Mccormick, A. V., & Flickinger, M. C. Separation Science and Technology, 32(15), 2547–2559.

By: M. Robichaud, A. Sathyagal, P. Carr, A. Mccormick & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Application of bioenergetics to modeling the microbial conversion of D-xylose to 2,3-butanediol

Jansen, N. B., Flickinger, M. C., & Tsao, G. T. Biotechnology and Bioengineering, 26(6), 573–582.

By: N. Jansen, M. Flickinger & G. Tsao

Source: NC State University Libraries
Added: August 6, 2018

journal article

Application of oxygen-enriched aeration in the conversion of glycerol to dihydroxyacetone by Gluconobacter melanogenus IFO 3293

Flickinger, M. C., & Perlman, D. Applied and Environmental Microbiology, 33(3), 706–712.

By: M. Flickinger & D. Perlman

Source: NC State University Libraries
Added: August 6, 2018

journal article

Application of oxygen-enriched aeration in the production of bacitracin by Bacillus licheniformis

Flickinger, M. C., & Perlman, D. Antimicrobial Agents and Chemotherapy, 15(2), 182–293.

By: M. Flickinger & D. Perlman

Source: NC State University Libraries
Added: August 6, 2018

journal article

Bacillus methanolicus pyruvate carboxylase and homoserine dehydrogenase I and II and their roles for l-lysine production from methanol at 50A degrees C

Brautaset, T., Jakobsen, O. M., Degnes, K. F., Netzer, R., Naerdal, I., Krog, A., … Ellingsen, T. E. Applied Microbiology and Biotechnology, 87(3), 951–964.

By: T. Brautaset, O. Jakobsen, K. Degnes, R. Netzer, I. Naerdal, A. Krog, R. Dillingham, M. Flickinger, T. Ellingsen

Source: NC State University Libraries
Added: August 6, 2018

journal article

Bacillus methanolicus: A candidate for industrial production of amino acids from methanol at 50 degrees C

Brautaset, T., Jakobsen, O. M., Josefsen, K. D., Flickinger, M. C., & Ellingsen, T. E. Applied Microbiology and Biotechnology, 74(1), 22–34.

By: T. Brautaset, O. Jakobsen, K. Josefsen, M. Flickinger & T. Ellingsen

Source: NC State University Libraries
Added: August 6, 2018

journal article

Bacterial expression and characterization of an anti-desipramine single-chain antibody fragment

Lin, G., Pentel, P. R., Shelver, W. L., Keyler, D. E., Ross, C. A., Hieda, Y., … Murtaugh, M. P. International Journal of Immunopharmacology, 18(12), 729–738.

By: G. Lin, P. Pentel, W. Shelver, D. Keyler, C. Ross, Y. Hieda, M. Flickinger, C. Pennell, M. Murtaugh

Source: NC State University Libraries
Added: August 6, 2018

journal article

Characterization of a restriction-modification system of the thermotolerant methylotroph Bacillus methanolicus

Cue, D., Lam, H., Hanson, R. S., & Flickinger, M. C. Applied and Environmental Microbiology, 62(3), 1107–1111.

By: D. Cue, H. Lam, R. Hanson & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Cloning, expression, and purification of an anti-desipramine single chain antibody in NS/0 myeloma cells

Kitchin, K., Lin, G., Shelver, W. L., Murtaugh, M. P., Pentel, P. R., Pond, S. M., … Flickinger, M. C. Journal of Pharmaceutical Sciences, 84(10), 1184–1189.

By: K. Kitchin, G. Lin, W. Shelver, M. Murtaugh, P. Pentel, S. Pond, J. Oberst, J. Humphrey, J. Smith, M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Comparison of fluoride modified zirconia with ceramic hydroxyapatite for preparative scale purification of immunoglobulin from serum albumin

Mullick, A., Griffith, C. M., Flickinger, M. C. M., A., & Flickinger, M. C. Preparative Biochemistry & Biotechnology, 28(1), 1–21.

By: A. Mullick, C. Griffith, M. Flickinger, . A. & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Conversion of hemicellulose carbohydrates

Gong, C. S., Chen, L. F., Flickinger, M. C., & Tsao, G. T. Advances in Biochemical Engineering, 20, 93–118.

By: C. Gong, L. Chen, M. Flickinger & G. Tsao

Source: NC State University Libraries
Added: August 6, 2018

journal article

Determination of protein expression and plasmid copy number from cloned genes in Escherichia coli by flow injection analysis using an enzyme indicator vector

Schendel, F. J., Baude, E. J., & Flickinger, M. C. Biotechnology and Bioengineering, 34(8), 1023–1036.

By: F. Schendel, E. Baude & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Determination of specific monoclonal antibody secretion rate during very slow hybridoma growth

Flickinger, M. C., Goebel, N. K., & Bohn, M. A. Bioprocess Engineering, 5(4), 155–164.

By: M. Flickinger, N. Goebel & M. Bohn

Source: NC State University Libraries
Added: August 6, 2018

journal article

Diffusion of nonadsorbing polymers within hierarchically structured colloidal aggregates.

Reeder, D. H., Carr, P. W., Flickinger, M. C., & McCormick, A. V. Journal of Colloid and Interface Science, 226(2), 277–285.

By: D. Reeder, P. Carr, M. Flickinger & A. McCormick

Source: NC State University Libraries
Added: August 6, 2018

journal article

Effects of recombinant drug-specific single chain antibody Fv fragment on [3H]-desipramine distribution in rats

Shelver, W. L., Keyler, D. E., Lin, G., Murtaugh, M. P., Flickinger, M. C., Ross, C. A., & Pentel, P. R. Biochemical Pharmacology, 51(4), 531–537.

By: W. Shelver, D. Keyler, G. Lin, M. Murtaugh, M. Flickinger, C. Ross, P. Pentel

Source: NC State University Libraries
Added: August 6, 2018

journal article

Electrochemical characterization of Geobacter sulfurreducens cells immobilized on graphite paper electrodes.

Srikanth, S., Marsili, E., Flickinger, M. C., & Bond, D. R. Biotechnology and Bioengineering, 99(5), 1065–1073.

By: S. Srikanth, E. Marsili, M. Flickinger & D. Bond

Source: NC State University Libraries
Added: August 6, 2018

journal article

Engineering and modeling of thin, adhesive, microbial biocatalytic coatings for high intensity oxidations in multi-phase microchannel bioreactors

Fidaleo, M., & Flickinger, M. C. Chemical Engineering Science, 66(14), 3251–3257.

By: M. Fidaleo & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Engineering the microstructure and permeability of thin multilayer latex biocatalytic coatings containing E. coli.

Lyngberg, O. K., Ng, C. P., Thiagarajan, V., Scriven, L. E., & Flickinger, M. C. Biotechnology Progress, 17(6), 1169–1179.

By: O. Lyngberg, C. Ng, V. Thiagarajan, L. Scriven & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Ethanol production from pentoses by immobilized microorganisms

Chiang, L. C., Hsiao, H. Y., Flickinger, M. C., Chen, L. F., & Tsao, G. T. Enzyme and Microbial Technology, 4(2), 93–95.

By: L. Chiang, H. Hsiao, M. Flickinger, L. Chen & G. Tsao

Source: NC State University Libraries
Added: August 6, 2018

journal article

Evidence for posttranscriptional stimulation of monoclonal antibody secretion by L-glutamine during slow hybridoma growth

Flickinger, M. C., Goebel, N. K., Bibila, T., & Boyce-Jacino, S. Journal of Biotechnology, 22(3), 201–226.

By: M. Flickinger, N. Goebel, T. Bibila & S. Boyce-Jacino

Source: NC State University Libraries
Added: August 6, 2018

journal article

Expanded bed adsorption of human serum albumin from very dense Saccharomyces cerevisiae suspensions on fluoride-modified zirconia

Mullick, A., & Flickinger, M. C. Biotechnology and Bioengineering, 65(3), 282–290.

By: A. Mullick & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Fuels and chemicals from biomass

Ladisch, M. R., Flickinger, M. C., & Tsao, G. T. Energy (Oxford, England), 4(2), 263–275.

By: M. Ladisch, M. Flickinger & G. Tsao

Source: NC State University Libraries
Added: August 6, 2018

journal article

Genetic manipulation of Bacillus methanolicus, a gram-positive, thermotolerant methylotroph

Cue, D., Lam, H., Dillingham, R. L., Hanson, R. S., & Flickinger, M. C. Applied and Environmental Microbiology, 63(4), 1406–1420.

By: D. Cue, H. Lam, R. Dillingham, R. Hanson & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Growth of Bacillus methanolicus in seawater-based media.

Komives, C. F., Cheung, L. Y.-Y., Pluschkell, S. B., & Flickinger, M. C. Journal of Industrial Microbiology & Biotechnology, 32(2), 61–66.

By: C. Komives, L. Cheung, S. Pluschkell & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

High-affinity CD25-binding IL-2 mutants potently stimulate persistent T Cell growth

Rao, B. M., Driver, I., Lauffenburger, D. A., & Wittrup, K. D. Biochemistry, 44(31), 10696–10701.

By: B. Rao, I. Driver, D. Lauffenburger & K. Wittrup

Source: NC State University Libraries
Added: August 6, 2018

journal article

Hydrogen production by photoreactive nanoporous latex coatings of nongrowing Rhodo-pseudomonas palustris CGA009

Gosse, J. L., Engel, B. J., Rey, F. E., Harwood, C. S., Scriven, L. E., & Flickinger, M. C. Biotechnology Progress, 23(1), 124–130.

By: J. Gosse, B. Engel, F. Rey, C. Harwood, L. Scriven & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Improved methods for investigating the external redox potential in hybridoma cell culture

Pluschkell, S. B., & Flickinger, M. C. Cytotechnology, 19(1), 11–26.

By: S. Pluschkell & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Inducible synthesis of the Mitomycin C resistance gene product (MCRA) from Streptomyces lavendulae

August, P. R., Rahn, J. A., Flickinger, M. C., & Sherman, D. H. Gene, 175(1/2), 261–267.

By: P. August, J. Rahn, M. Flickinger & D. Sherman

Source: NC State University Libraries
Added: August 6, 2018

journal article

Kinetic properties of enzyme populations in vivo: alkaline phosphatase of the Escherichia coli periplasm

Martinez, M. B., Schendel, F. J., Flickinger, M. C., & Nelsestuen, G. L. Biochemistry, 31(46), 11500–11509.

By: M. Martinez, F. Schendel, M. Flickinger & G. Nelsestuen

Source: NC State University Libraries
Added: August 6, 2018

journal article

Kinetics of dehydration of aqueous 2,3-butanediol to methyl ethyl ketone

Emerson, R. R., Flickinger, M. C., & Tsao, G. T. Industrial & Engineering Chemistry Product Research and Development, 21(3), 473–477.

By: R. Emerson, M. Flickinger & G. Tsao

Source: NC State University Libraries
Added: August 6, 2018

journal article

Large-scale purification of a murine antimelanoma monoclonal antibody

Lee, S. M., Gustafson, M. E., Pickle, D. J., Flickinger, M. C., Muschik, G. M., & Morgan, A. C., Jr. Journal of Biotechnology, 4(4), 189–204.

By: S. Lee, M. Gustafson, D. Pickle, M. Flickinger, G. Muschik & A. Morgan

Source: NC State University Libraries
Added: August 6, 2018

journal article

Lysine production from methanol at 50 degrees C using Bacillus methanolicus: Modeling volume control, lysine concentration, and productivity using a three-phase continuous simulation

Lee, G. H., Hur, W., Bremmon, C. E., & Flickinger, M. C. Biotechnology and Bioengineering, 49(6), 639–653.

By: G. Lee, W. Hur, C. Bremmon & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Method for construction of a aimple laboratory-scale nonwoven filament biocatalytic filter

Flickinger, M. C., Mullick, A., & Ollis, D. F. Biotechnology Progress, 14(4), 664–666.

By: M. Flickinger, A. Mullick & D. Ollis

Source: NC State University Libraries
Added: August 6, 2018

journal article

Microstructure evolution in polymer latex coatings for whole-cell biocatalyst application

Huang, Z., Thiagarajan, V. S., Lyngberg, O. K., Scriven, L. E., & Flickinger, M. C. Journal of Colloid and Interface Science, 215(2), 226–243.

By: Z. Huang, V. Thiagarajan, O. Lyngberg, L. Scriven & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Microstructure of a biocatalytic latex coating containing viable Escherichia coli cells

Thiagarajan, V. S., Huang, Z., Scriven, L. E., Schottel, J. L., & Flickinger, M. C. Journal of Colloid and Interface Science, 215(2), 244–257.

By: V. Thiagarajan, Z. Huang, L. Scriven, J. Schottel & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Mobile self-contained off-gas analysis units for fermentation pilot plants

Flickinger, M. C., Jansen, N. B., & Forrest, E. H. Biotechnology and Bioengineering, 22(6), 1279–1276.

By: M. Flickinger, N. Jansen & E. Forrest

Source: NC State University Libraries
Added: August 6, 2018

journal article

Modelling diffusivity in porous polymeric membranes with an intermediate layer containing microbial cells.

Mota, M., Yelshin, A., Fidaleo, M., & Flickinger, M. C. Biochemical Engineering Journal, 37(3), 285–293.

By: M. Mota, A. Yelshin, M. Fidaleo & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Monitoring growth and acetic acid secretion by a thermotolerant Bacillus using conduction microcalorimetry

Anderson, C. R., & Flickinger, M. C. Journal of Industrial Microbiology, 12(2), 114–120.

By: C. Anderson & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Null mutation in the stringent starvation protein of Escherichia coli disrupts lytic development of bacteriophage P1

Williams, M. D., Fuchs, J. A., & Flickinger, M. C. Gene, 109(1), 21–30.

By: M. Williams, J. Fuchs & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Overexpression of wild-type aspartokinase increases L-lysine production in the thermotolerant methylotrophic bacterium Bacillus methanolicus.

Jakobsen, O. M., Brautaset, T., Degnes, K. F., Heggeset, T. M. B., Balzer, S., Flickinger, M. C., … Ellingsen, T. E. Applied and Environmental Microbiology, 75(3), 652–661.

By: O. Jakobsen, T. Brautaset, K. Degnes, T. Heggeset, S. Balzer, M. Flickinger, S. Valla, T. Ellingsen

Source: NC State University Libraries
Added: August 6, 2018

journal article

Painting and printing living bacteria: Engineering nanoporous biocatalytic coatings to preserve microbial viability and intensify reactivity.

Flickinger, M. C., Schottel, J. L., Bond, D. R., Aksan, A., & Scriven, L. E. Biotechnology Progress, 23(1), 2–17.

By: M. Flickinger, J. Schottel, D. Bond, A. Aksan & L. Scriven

Source: NC State University Libraries
Added: August 6, 2018

journal article

Permeability and reactivity of Thermotoga maritima in latex bimodal blend coatings at 80 degrees C: a model high temperature biocatalytic coating.

Lyngberg, O. K., Solheid, C., Charaniya, S., Ma, Y., Thiagarajan, V., Scriven, L. E., & Flickinger, M. C. Extremophiles, 9(3), 197–207.

By: O. Lyngberg, C. Solheid, S. Charaniya, Y. Ma, V. Thiagarajan, L. Scriven, M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Pilot- and production-scale containment of cytotoxic and oncogenic fermentation processes

Flickinger, M. C., & Sansone, E. B. Biotechnology and Bioengineering, 26(8), 860–870.

By: M. Flickinger & E. Sansone

Source: NC State University Libraries
Added: August 6, 2018

journal article

Plasmid-dependent methylotrophy in thermotolerant Bacillus methanolicus.

Brautaset, T., Jakobsen, O. M., Flickinger, M. C., Valla, S., & Ellingsen, T. E. Journal of Bacteriology, 186(5), 1229–1238.

By: T. Brautaset, O. Jakobsen, M. Flickinger, S. Valla & T. Ellingsen

Source: NC State University Libraries
Added: August 6, 2018

journal article

Pore structure and diffusion tortuosity of porous ZrO2 synthesized by two different colloid-aggregation processes

Lorenzano-Porras, C. F., Annen, M. J., Flickinger, M. C., Carr, P. W., & McCormick, A. V. Journal of Colloid and Interface Science, 170(2), 299–307.

By: C. Lorenzano-Porras, M. Annen, M. Flickinger, P. Carr & A. McCormick

Source: NC State University Libraries
Added: August 6, 2018

journal article

Preliminary assessment of removal of pyrogenic lipopolysaccharides with colloidal zirconia adsorbents

Karl, D. W., Magnusson, J. C., Carr, P. W., & Flickinger, M. C. Enzyme and Microbial Technology, 13(9), 708–715.

By: D. Karl, J. Magnusson, P. Carr & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Production of 2,3-butanediol from D-xylose by Klebsiella oxytoca ATCC 8724

Jansen, N. B., Flickinger, M. C., & Tsao, G. T. Biotechnology and Bioengineering, 26(4), 362–368.

By: N. Jansen, M. Flickinger & G. Tsao

Source: NC State University Libraries
Added: August 6, 2018

journal article

Progress toward a biomimetic leaf: 4,000 h of hydrogen production by coating-stabilized Nongrowing Photosynthetic <i>Rhodopseudomonas palustris</i>

Gosse, J. L., Engel, B. J., Hui, J. C. H., Harwood, C. S., & Flickinger, M. C. Biotechnology Progress, 26(4), 907–918.

By: J. Gosse, B. Engel, J. Hui, C. Harwood & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Rapid analytical extraction of volatile fermentation products

Flickinger, M. C., & Tsao, G. T. Biotechnology and Bioengineering, 21(10), 1881–1883.

By: M. Flickinger & G. Tsao

Source: NC State University Libraries
Added: August 6, 2018

journal article

Reduced outer membrane permeability of escherichia coli O157:H7: Suggested role of modified outer membrane porins and theoretical function in resistance to antimicrobial agents.

Martinez, M. B., Flickinger, M., Higgins, L., Krick, T., & Nelsestuen, G. L. Biochemistry, 40(40), 11965–11974.

By: M. Martinez, M. Flickinger, L. Higgins, T. Krick & G. Nelsestuen

Source: NC State University Libraries
Added: August 6, 2018

journal article

Role of the Bacillus methanolicus citrate synthase II gene, citY, in regulating the secretion of glutamate in L-lysine-secreting mutants.

Brautaset, T., Williams, M. D., Dillingham, R. D., Kaufmann, C., Bennaars, A., Crabbe, E., & Flickinger, M. C. Applied and Environmental Microbiology, 69(7), 3986–3995.

By: T. Brautaset, M. Williams, R. Dillingham, C. Kaufmann, A. Bennaars, E. Crabbe, M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Spatial expression of a mercury-inducible green fluorescent protein within a nanoporous latex-based biosensor coating

Schottel, J. L., Orwin, P. M., Anderson, C. R., & Flickinger, M. C. Journal of Industrial Microbiology & Biotechnology, 35(4), 283–290.

By: J. Schottel, P. Orwin, C. Anderson & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Starvation-induced expression of SspA and SspB: the effects of a null mutation in sspA on Escherichia coli protein synthesis and survival during growth and prolonged starvation

Williams, M. D., Ouyang, T. X., & Flickinger, M. C. Molecular Microbiology, 11(6), 1039–1043.

By: M. Williams, T. Ouyang & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Steady-state enzyme kinetics in the Escherichia coli periplasm: a model of a whole cell biocatalyst

Martinez, M. B., Flickinger, M. C., & Nelsestuen, G. L. Journal of Biotechnology, 71(1-3), 59–66.

By: M. Martinez, M. Flickinger & G. Nelsestuen

Source: NC State University Libraries
Added: August 6, 2018

journal article

Strain selection, medium development and scale-up of toyocamycin production by Streptomyces chrestomyceticus

Flickinger, M. C., Greenstein, M., Bremmon, C., & Conlin, J. Bioprocess Engineering, 5(4), 143–153.

By: M. Flickinger, M. Greenstein, C. Bremmon & J. Conlin

Source: NC State University Libraries
Added: August 6, 2018

journal article

Sustaining protein synthesis in the absence of rapid cell division: An investigation of plasmid-encoded protein expression in Escherichia coli during very slow growth

Flickinger, M. C., & Rouse, M. P. Biotechnology Progress, 9(6), 555–572.

By: M. Flickinger & M. Rouse

Source: NC State University Libraries
Added: August 6, 2018

journal article

The development of an immobilized lactate oxidase system for lactic acid analysis

Cannon, J. J., Chen, L. F., Flickinger, M. C., & Tsao, G. T. Biotechnology and Bioengineering, 26(2), 167–173.

By: J. Cannon, L. Chen, M. Flickinger & G. Tsao

Source: NC State University Libraries
Added: August 6, 2018

journal article

The efficient removal of endotoxins from insulin using quaternized polyethylenimine-coated porous zirconia

McNeff, C., Zhao, Q., Almlof, E., Flickinger, M., & Carr, P. W. Analytical Biochemistry, 274(2), 181–187.

By: C. McNeff, Q. Zhao, E. Almlof, M. Flickinger & P. Carr

Source: NC State University Libraries
Added: August 6, 2018

journal article

The use of confocal scanning laser microscopy and other tools to characterize Escherichia coli in a high-cell-density synthetic biofilm

Swope, K., & Flickinger, M. C. Biotechnology and Bioengineering, 52(2), 340–356.

By: K. Swope & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Thermodynamic feasibility of enzymatic reduction of carbon dioxide to methanol

Baskaya, F. S., Zhao, X. Y., Flickinger, M. C., & Wang, P. Applied Biochemistry and Biotechnology, 162(2), 391–398.

By: F. Baskaya, X. Zhao, M. Flickinger & P. Wang

Source: NC State University Libraries
Added: August 6, 2018

journal article

Uniform lab-scale biocatalytic nanoporous latex coatings for reactive microorganisms

Gosse, J. L., & Flickinger, M. C. Methods in Molecular Biology, 743, 213–222.

By: J. Gosse & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Upregulated transcription of plasmid and chromosomal ribulose monophosphate pathway genes is critical for methanol assimilation rate and methanol tolerance in the methylotrophic bacterium Bacillus methanolicus

Jakobsen, O. M., Benichou, A., Flickinger, M. C., Valla, S., Ellingsen, T. E., & Brautaset, T. Journal of Bacteriology, 188(8), 3063–3072.

By: O. Jakobsen, A. Benichou, M. Flickinger, S. Valla, T. Ellingsen & T. Brautaset

Source: NC State University Libraries
Added: August 6, 2018

journal article

Use of a structured kinetic model of antibody synthesis and secretion for optimization of antibody production systems: I. Steady-state analysis

Bibila, T. A., & Flickinger, M. C. Biotechnology and Bioengineering, 39(3), 251–261.

By: T. Bibila & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Use of a structured kinetic model of antibody synthesis and secretion for optimization of antibody production systems: II. Transient analysis

Bibila, T. A., & Flickinger, M. C. Biotechnology and Bioengineering, 39(3), 262–272.

By: T. Bibila & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

journal article

Use of the weighted jackknife method to calculate the variance in cellular-specific protein secretion rate: application to monoclonal antibody secretion rate kinetics in response to osmotic stress

Yang, X., Oehlert, G. W., & Flickinger, M. C. Biotechnology and Bioengineering, 50(2), 184–196.

By: X. Yang, G. Oehlert & M. Flickinger

Source: NC State University Libraries
Added: August 6, 2018

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