Melissa Christina Srougi

Also known as: Melissa S. Bentle

Works (28)

Updated: August 20th, 2025 21:27

2025 article

Abstract 2353 Using Immersive Technologies to Teach Technical Skills in Biotechnology

Srougi, M., Cartwright, E., Spencer, D., McKeown, C., Tredwell, D., Potts, C., & Jhala, A. (2025, May 1). Journal of Biological Chemistry, Vol. 301.

By: M. Srougi* , E. Cartwright*, D. Spencer* , C. McKeown*, D. Tredwell*, C. Potts*, A. Jhala* 

author keywords: biotechnology; mammalian cell culture; laboratory training; immersive; interactive; virtual
topics (OpenAlex): Biomedical and Engineering Education
Sources: Web Of Science, NC State University Libraries
Added: July 21, 2025

2025 article

Abstract 2371 Enhancing Molecular Biotechnology Laboratory Education for Diverse Institutions

Srougi, M., Corbett, A., Garcia, C., Hayward, J., Sabaoun, M., Santisteban, M., … Kelly, R. (2025, May 1). Journal of Biological Chemistry, Vol. 301.

By: M. Srougi* , A. Corbett, C. Garcia, J. Hayward, M. Sabaoun, M. Santisteban, V. Sivaraman, C. Goller* , R. Kelly* 

author keywords: Science education; molecular biotechnology; laboratory training
topics (OpenAlex): Biomedical and Engineering Education
Sources: Web Of Science, NC State University Libraries
Added: July 21, 2025

2024 article

Abstract 1497 Development and Evaluation of an Immersive and Interactive Virtual Cell Culture Training for Accessible Biotechnology

Srougi, M., Spencer, D., Cartwright, E., McKeown, C., Potts, C., & Jhala, A. (2024, March 1). Journal of Biological Chemistry, Vol. 300, pp. S79–S79.

By: M. Srougi n , D. Spencer* , E. Cartwright*, C. McKeown*, C. Potts* & A. Jhala* 

topics (OpenAlex): Biomedical and Engineering Education
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9. Industry, Innovation and Infrastructure (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: December 2, 2024

2024 article

Student experiences with a molecular biotechnology course containing an interactive 3D immersive simulation and its impact on motivational beliefs

Spencer, D., McKeown, C., Tredwell, D., Huckaby, B., Wiedner, A., Dums, J. T., … Srougi, M. C. (2024, July 25). (R. Lavi, Ed.). PLoS ONE, Vol. 19.

By: D. Spencer n , C. McKeown n, D. Tredwell n, B. Huckaby n, A. Wiedner n, J. Dums n, E. Cartwright n, C. Potts n ...

Ed(s): R. Lavi

MeSH headings : Humans; Students / psychology; Motivation; Biotechnology / education; Biotechnology / methods; Male; Female; Virtual Reality; Young Adult; Adult; Computer Simulation
topics (OpenAlex): Career Development and Diversity; Psychological and Educational Research Studies; Educational Strategies and Epistemologies
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4. Quality Education (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 14, 2024

2023 article

Abstract 1931: Innovating Life Sciences Laboratory Training: Molecular Biology Laboratory Education Modules (MBLEMs) as a Model for Advanced Training at Diverse Institutions

Srougi, M., Corbett, A., Garcia, C., Sabaoun, M., Santisteban, M., Sivaraman, V., … Kelly, R. (2023, January 1). Journal of Biological Chemistry, Vol. 299, pp. S260–S260.

By: M. Srougi* , A. Corbett, C. Garcia, M. Sabaoun, M. Santisteban, V. Sivaraman, S. Chen* , C. Goller* , R. Kelly* 

topics (OpenAlex): Biomedical and Engineering Education; Genetics, Bioinformatics, and Biomedical Research
Sources: Web Of Science, NC State University Libraries
Added: September 11, 2023

2023 article

Design and Implementation of an Accessible and Open-Sourced In Silico Drug Screening Activity for Cancer Drug Discovery

Fikes, A. G., & Srougi, M. C. (2023, September 19). Journal of Chemical Education, Vol. 100, pp. 4125–4130.

By: A. Fikes n  & M. Srougi n 

author keywords: Graduate Education/Research; Upper-Division Undergraduate; Interdisciplinary/Multidisciplinary; Biochemistry; Chemoinformatics; Computer-Based Learning; Internet/Web-Based Learning; Biotechnology; Drugs/Pharmaceuticals; Molecular Modeling
topics (OpenAlex): Computational Drug Discovery Methods; Enzyme function and inhibition; Various Chemistry Research Topics; Genetics, Bioinformatics, and Biomedical Research
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3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: October 16, 2023

2021 article

Growth mindset interventions improve academic performance but not mindset in biochemistry

Miller, H. B., & Srougi, M. C. (2021, July 15). Biochemistry and Molecular Biology Education.

By: H. Miller * & M. Srougi n 

author keywords: academic performance; biochemistry; growth mindset; metacognition
MeSH headings : Academic Performance; Attitude; Humans; Learning; Students; Universities
topics (OpenAlex): Education, Achievement, and Giftedness; Educational Strategies and Epistemologies; Grit, Self-Efficacy, and Motivation
TL;DR: The results suggest that although students' mindsets did not change as a result of growth mindset interventions, their positive perceptions about learning versus performance did increase and they significantly outperformed students who did not receive interventions on the final cumulative exam that assessed critical thinking skills. (via Semantic Scholar)
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4. Quality Education (OpenAlex)
Source: ORCID
Added: July 17, 2021

2021 journal article

Integrating Bioinformatics Tools into Inquiry-based Molecular Biology Laboratory Education Modules

Frontiers in Education, 6.

By: C. Goller n , M. Srougi n , S. Chen n , L. Schenkman n & R. Kelly n 

Contributors: C. Goller n , M. Srougi n , S. Chen n , L. Schenkman n & R. Kelly n 

author keywords: bioinformatics; science education; molecular biotechnology; software tools; case studies
topics (OpenAlex): Genetics, Bioinformatics, and Biomedical Research; Biomedical and Engineering Education
TL;DR: This new paradigm for biology laboratory education that embraces bioinformatics as a critical component of inquiry-based learning for undergraduate and graduate students representing the life sciences, the physical sciences, and engineering is described. (via Semantic Scholar)
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4. Quality Education (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 23, 2021

2020 article

Using Metacognitive Strategies to Improve Academic Performance in Biochemistry

Miller, H. B., & Srougi, M. C. (2020, July 9).

By: H. Miller * & M. Srougi n 

topics (OpenAlex): Education, Achievement, and Giftedness; Grit, Self-Efficacy, and Motivation; Educational Strategies and Epistemologies
TL;DR: Although students’ mindsets did not change as a result of metacognitive interventions, their positive perceptions about learning vs performance did increase and students receiving growth mindset interventions significantly outperformed students who did not receive interventions on the final cumulative exam that assessed critical thinking skills. (via Semantic Scholar)
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4. Quality Education (OpenAlex)
Source: ORCID
Added: June 25, 2021

2019 article

Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress

Tolbert, C. E., Palmquist, L., Dixon, H. L., & Srougi, M. C. (2019, October 13). Journal of Visualized Experiments, Vol. 10.

By: C. Tolbert*, L. Palmquist *, H. Dixon* & M. Srougi n 

author keywords: Biochemistry; Issue 152; cell adhesion; cellular spreading; oxidative stress; fibronectin; epithelial cells; ATM inhibition; ruffles; stress fibers; cell circularity
MeSH headings : Animals; Cell Adhesion / drug effects; Cell Adhesion / physiology; Cell Count / methods; Cell Culture Techniques / methods; Cell Line; Cell Line, Transformed; Cells, Cultured; Epithelial Cells / drug effects; Epithelial Cells / metabolism; Extracellular Matrix / drug effects; Extracellular Matrix / metabolism; Fibroblasts / drug effects; Fibroblasts / metabolism; Fibronectins / metabolism; Humans; Microscopy, Fluorescence / methods; Morpholines / pharmacology; Oxidative Stress / drug effects; Oxidative Stress / physiology; Pyrones / pharmacology
topics (OpenAlex): Cell Adhesion Molecules Research
TL;DR: A step-wise protocol is described that utilizes an immunofluorescence-based assay to specifically quantify cell adhesion and spreading of immortalized fibroblast cells on fibronectin (FN) in vitro to examine a broad range of biological questions. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: November 18, 2019

2019 book

Molecular Biology Techniques 4th Edition

In Elsevier. https://www.elsevier.com/books/molecular-biology-techniques/carson/978-0-12-815774-9

By: S. Carson, H. Miller, M. Srougi  & S. Witherow

www.elsevier.com
Source: ORCID
Added: June 25, 2021

2018 article

Loss of ATM positively regulates Rac1 activity and cellular migration through oxidative stress

Tolbert, C. E., Beck, M. V., Kilmer, C. E., & Srougi, M. C. (2018, December 13). Biochemical and Biophysical Research Communications, Vol. 508, pp. 1155–1161.

By: C. Tolbert*, M. Beck*, C. Kilmer n & M. Srougi* 

author keywords: ATM; Rac1; Reactive oxygen species; Migration
MeSH headings : Animals; Ataxia Telangiectasia Mutated Proteins / metabolism; Cell Movement; Enzyme Activation; HeLa Cells; Humans; Oxidative Stress; Rats; Reactive Oxygen Species / metabolism; rac1 GTP-Binding Protein / metabolism
topics (OpenAlex): DNA Repair Mechanisms; Microtubule and mitosis dynamics; Cancer-related Molecular Pathways
TL;DR: It is shown that loss of ATM kinase activity elevates intracellular ROS, leading to Rac1 activation, which drives cytoskeletal rearrangements resulting in increased cellular spreading and motility. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: April 20, 2020

2018 article

Student-Designed High-Throughput Assays to Assess Effects of Growth Insults in Budding Yeast

Segarra, V. A., Wilson, C., Lowery, K., Ransdell, S., Schnuck, J., Way, P., & Srougi, M. C. (2018, January 1). Journal of Microbiology and Biology Education.

By: V. Segarra *, C. Wilson*, K. Lowery*, S. Ransdell*, J. Schnuck *, P. Way*, M. Srougi* 

topics (OpenAlex): Biomedical and Engineering Education; Genetics, Bioinformatics, and Biomedical Research
TL;DR: An open inquiry-based laboratory activity exploiting a small-scale high-throughput screening assay of the budding yeast S. cerevisiae is designed to promote active learning and introduce students to modern data collection and analysis techniques. (via Semantic Scholar)
Source: ORCID
Added: June 25, 2021

2017 article

Peer learning as a tool to strengthen math skills in introductory chemistry laboratories

Srougi, M. C., & Miller, H. B. (2017, November 20). Chemistry Education Research and Practice, Vol. 19, pp. 319–330.

By: M. Srougi*  & H. Miller *

topics (OpenAlex): Innovative Teaching and Learning Methods; Innovative Teaching Methods; Various Chemistry Research Topics
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4. Quality Education (OpenAlex)
Source: ORCID
Added: June 25, 2021

2014 journal article

Making Heads or Tails: Planarian Stem Cells in the Classroom †

Journal of Microbiology & Biology Education, 15(1), 18–25.

By: M. Srougi n , S. Carson n , J. Thomas-Swanik*, J. Marchant * & J. Chan *

topics (OpenAlex): Planarian Biology and Electrostimulation; 3D Printing in Biomedical Research; Pluripotent Stem Cells Research
TL;DR: A hands-on introductory laboratory module that introduces students to stem cell biology and can be easily incorporated into existing curricula is developed and implemented and piloted at the high school, community college, and university levels. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
4. Quality Education (OpenAlex)
14. Life Below Water (Web of Science)
Sources: ORCID, Crossref, NC State University Libraries
Added: January 19, 2020

2014 article

Tumor-Selective, Futile Redox Cycle-Induced Bystander Effects Elicited by NQO1 Bioactivatable Radiosensitizing Drugs in Triple-Negative Breast Cancers

Cao, L., Li, L. S., Spruell, C., Xiao, L., Chakrabarti, G., Bey, E. A., … Boothman, D. A. (2014, February 10). Antioxidants and Redox Signaling.

By: L. Cao *, L. Li*, C. Spruell*, L. Xiao *, G. Chakrabarti*, E. Bey *, K. Reinicke*, M. Srougi*  ...

MeSH headings : Animals; Bystander Effect / drug effects; Female; Humans; Mice; Mice, Nude; NAD(P)H Dehydrogenase (Quinone) / deficiency; NAD(P)H Dehydrogenase (Quinone) / genetics; NAD(P)H Dehydrogenase (Quinone) / metabolism; Naphthoquinones / pharmacology; Oxidation-Reduction / drug effects; Quinones / pharmacology; Radiation-Sensitizing Agents / pharmacology; Triple Negative Breast Neoplasms / drug therapy; Triple Negative Breast Neoplasms / metabolism; Triple Negative Breast Neoplasms / pathology; Tumor Cells, Cultured; Xenograft Model Antitumor Assays
topics (OpenAlex): Bioactive Compounds and Antitumor Agents; Free Radicals and Antioxidants; Coenzyme Q10 studies and effects
TL;DR: Bystander effects elicited by NQO1 bioactivatable drugs (β-lap or deoxynyboquinone [DNQ]) likely contribute to their efficacies, killing NQo1(+) solid cancer cells and eliminating surrounding heterogeneous N QO1(low) cancer cells. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: June 25, 2021

2013 article

Assessment of a novel group‐centered testing schema in an upper‐level undergraduate molecular biotechnology course

Srougi, M. C., Miller, H. B., Witherow, D. S., & Carson, S. (2013, July 1). Biochemistry and Molecular Biology Education, Vol. 41, pp. 232–241.

By: M. Srougi n , H. Miller *, D. Witherow * & S. Carson n 

author keywords: biotechnology; problem-based testing; collaborative learning; undergraduate
MeSH headings : Biotechnology / education; Curriculum; Educational Measurement / methods; Faculty; Humans; Learning; Students
topics (OpenAlex): Problem and Project Based Learning; Education and Critical Thinking Development; Innovative Teaching Methods
TL;DR: It is suggested that group‐centered, problem‐based learning is a useful model for achievement of student learning outcomes in courses where it would be infeasible to provide feedback on individual critical thinking assignments due to grading volume. (via Semantic Scholar)
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4. Quality Education (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2013 article

Catalase Abrogates β-Lapachone–Induced PARP1 Hyperactivation–Directed Programmed Necrosis in NQO1-Positive Breast Cancers

Bey, E. A., Reinicke, K. E., Srougi, M. C., Varnes, M., Anderson, V. E., Pink, J. J., … Boothman, D. A. (2013, July 25). Molecular Cancer Therapeutics.

By: E. Bey *, K. Reinicke*, M. Srougi* , M. Varnes*, V. Anderson *, J. Pink *, L. Li*, M. Patel* ...

MeSH headings : Breast Neoplasms / drug therapy; Breast Neoplasms / genetics; Breast Neoplasms / pathology; Catalase / genetics; Catalase / metabolism; DNA Breaks, Single-Stranded / drug effects; DNA Damage / drug effects; Female; Gene Expression Regulation, Neoplastic / drug effects; Humans; Hydrogen Peroxide / metabolism; NAD(P)H Dehydrogenase (Quinone) / genetics; NAD(P)H Dehydrogenase (Quinone) / metabolism; Naphthoquinones / administration & dosage; Necrosis / genetics; Necrosis / pathology; Poly (ADP-Ribose) Polymerase-1; Poly(ADP-ribose) Polymerases / genetics; Reactive Oxygen Species / metabolism
topics (OpenAlex): Bioactive Compounds and Antitumor Agents; Cancer therapeutics and mechanisms
TL;DR: Although PARP1 hyperactivation and NQO1 expression were major determinants of β-lapachone–induced lethality, alterations in catalase expression, including treatment with exogenous enzyme, caused marked cytoprotection, andCatalase is an important resistance factor and highlights H2O2 as an obligate ROS for cell death from this agent. (via Semantic Scholar)
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3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: June 25, 2021

2013 journal article

Inquiry into chemotherapy-induced p53 activation in cancer cells as a model for teaching signal transduction

Biochemistry and Molecular Biology Education, 41(6), 419–432.

By: M. Srougi n  & S. Carson n 

author keywords: cancer; laboratory; cell signaling; p53
MeSH headings : Antineoplastic Agents, Phytogenic / pharmacology; Biochemistry / education; Blotting, Western; Cell Line, Tumor; Cell Survival / drug effects; Drug Therapy / methods; Etoposide / pharmacology; Flow Cytometry; Humans; Medical Laboratory Personnel / education; Microscopy, Fluorescence; Neoplasms / metabolism; Neoplasms / pathology; Phosphorylation / drug effects; Problem-Based Learning / methods; Reproducibility of Results; Research / education; Research / instrumentation; Research Design / standards; Research Report / standards; Signal Transduction / drug effects; Students; Teaching / methods; Tumor Suppressor Protein p53 / metabolism; Universities
topics (OpenAlex): CAR-T cell therapy research; Cancer therapeutics and mechanisms
TL;DR: A new laboratory‐intensive course that teaches students the theory and techniques used to study cell signaling pathways as they conduct a hypothesis‐driven research project to elucidate the mechanism of breast cancer cell death caused by a cancer chemotherapeutic agent is developed and implemented. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

2011 article

The Nuclear Guanine Nucleotide Exchange Factors Ect2 and Net1 Regulate RhoB-Mediated Cell Death after DNA Damage

Srougi, M. C., & Burridge, K. (2011, February 23). PLoS ONE.

By: M. Srougi*  & K. Burridge *

MeSH headings : Breast Neoplasms / genetics; Breast Neoplasms / metabolism; Breast Neoplasms / pathology; Carcinoma / genetics; Carcinoma / metabolism; Carcinoma / pathology; Cell Death / drug effects; Cell Death / genetics; Cell Line, Transformed; DNA Damage / drug effects; DNA Damage / genetics; Female; Gene Expression Regulation, Neoplastic / drug effects; Guanine Nucleotide Exchange Factors / antagonists & inhibitors; Guanine Nucleotide Exchange Factors / genetics; Guanine Nucleotide Exchange Factors / metabolism; Guanine Nucleotide Exchange Factors / physiology; HeLa Cells; Humans; Oncogene Proteins / antagonists & inhibitors; Oncogene Proteins / genetics; Oncogene Proteins / metabolism; Oncogene Proteins / physiology; Proto-Oncogene Proteins / antagonists & inhibitors; Proto-Oncogene Proteins / genetics; Proto-Oncogene Proteins / metabolism; Proto-Oncogene Proteins / physiology; RNA, Small Interfering / pharmacology; Tumor Cells, Cultured; rhoB GTP-Binding Protein / antagonists & inhibitors; rhoB GTP-Binding Protein / genetics; rhoB GTP-Binding Protein / metabolism; rhoB GTP-Binding Protein / physiology
topics (OpenAlex): Cell death mechanisms and regulation; Protein Kinase Regulation and GTPase Signaling; Cancer-related Molecular Pathways
TL;DR: A mechanism involving the nuclear GEFs Ect2 and Net1 for activating RhoB after genotoxic stress is suggested, thereby facilitating cell death after treatment with DNA damaging agents. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: June 25, 2021

2011 article

The Small GTPase RhoA Localizes to the Nucleus and Is Activated by Net1 and DNA Damage Signals

Dubash, A. D., Guilluy, C., Srougi, M. C., Boulter, E., Burridge, K., & García-Mata, R. (2011, February 24). PLoS ONE.

By: A. Dubash *, C. Guilluy *, M. Srougi* , E. Boulter *, K. Burridge * & R. García-Mata *

MeSH headings : Cell Nucleus / drug effects; Cell Nucleus / metabolism; Cells, Cultured; DNA Damage / physiology; Guanine Nucleotide Exchange Factors / metabolism; HeLa Cells; Humans; Monomeric GTP-Binding Proteins / metabolism; Oncogene Proteins / antagonists & inhibitors; Oncogene Proteins / genetics; Oncogene Proteins / metabolism; Oncogene Proteins / physiology; Protein Transport / drug effects; RNA, Small Interfering / pharmacology; Signal Transduction / drug effects; Signal Transduction / physiology; Tissue Distribution / drug effects; rhoA GTP-Binding Protein / metabolism
topics (OpenAlex): Protein Kinase Regulation and GTPase Signaling; PI3K/AKT/mTOR signaling in cancer
TL;DR: These results demonstrate the existence of a functional Net1/RhoA signaling pathway within the nucleus of the cell and implicate them in the DNA damage response. (via Semantic Scholar)
Source: ORCID
Added: June 25, 2021

2009 article

Undressing a cellular corset: septins exposed

Srougi, M. C., & Burridge, K. (2009, January 1). Nature Cell Biology.

By: M. Srougi*  & K. Burridge *

MeSH headings : Animals; Cell Movement / physiology; Cell Polarity / physiology; Cell Shape / physiology; Cell Surface Extensions / metabolism; Cell Surface Extensions / ultrastructure; Chemotaxis, Leukocyte / physiology; Cytoskeletal Proteins / metabolism; GTP Phosphohydrolases / metabolism; Humans; Septins; T-Lymphocytes / metabolism; T-Lymphocytes / ultrastructure
topics (OpenAlex): Cellular Mechanics and Interactions; Advanced Proteomics Techniques and Applications; Fungal and yeast genetics research
TL;DR: A new study identifies a previously undefined role for septins as cellular 'corsets', modulating cell shape and providing cortical rigidity in T cells that allows for their controlled migration. (via Semantic Scholar)
Source: ORCID
Added: June 25, 2021

2007 article

An NQO1- and PARP-1-mediated cell death pathway induced in non-small-cell lung cancer cells by β-lapachone

Bey, E. A., Bentle, M. S., Reinicke, K. E., Dong, Y., Yang, C.-R., Girard, L., … Boothman, D. A. (2007, July 4). Proceedings of the National Academy of Sciences.

By: E. Bey *, M. Bentle*, K. Reinicke*, Y. Dong *, C. Yang*, L. Girard *, J. Minna *, W. Bornmann*, J. Gao *, D. Boothman *

author keywords: DNA repair inhibitor; non-small-cell lung cancer; NQO1; apoptosis; mu-calpain cell death
MeSH headings : Antineoplastic Agents / pharmacology; Apoptosis / drug effects; Apoptosis / physiology; Carcinoma, Non-Small-Cell Lung / drug therapy; Carcinoma, Non-Small-Cell Lung / enzymology; Carcinoma, Non-Small-Cell Lung / metabolism; Cell Line; Cell Line, Tumor; Humans; Lung Neoplasms / drug therapy; Lung Neoplasms / enzymology; Lung Neoplasms / metabolism; NAD(P)H Dehydrogenase (Quinone) / biosynthesis; NAD(P)H Dehydrogenase (Quinone) / genetics; NAD(P)H Dehydrogenase (Quinone) / physiology; Naphthoquinones / pharmacology; Poly (ADP-Ribose) Polymerase-1; Poly(ADP-ribose) Polymerases / physiology; Signal Transduction / drug effects; Signal Transduction / physiology; Tumor Cells, Cultured
topics (OpenAlex): Bioactive Compounds and Antitumor Agents; Cancer therapeutics and mechanisms; Synthesis and Biological Evaluation
TL;DR: The data indicate that the most efficacious strategy using β-lapachone in chemotherapy was to deliver the drug in short pulses, greatly reducing cytotoxicity to NQO1− “normal” cells. (via Semantic Scholar)
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3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: June 25, 2021

2007 article

Nonhomologous End Joining Is Essential for Cellular Resistance to the Novel Antitumor Agent, β-Lapachone

Bentle, M. S., Reinicke, K. E., Dong, Y., Bey, E. A., & Boothman, D. A. (2007, July 15). Cancer Research.

By: M. Bentle*, K. Reinicke*, Y. Dong *, E. Bey * & D. Boothman *

MeSH headings : Antineoplastic Agents / pharmacology; Cell Line, Tumor; Cell Survival; Comet Assay; DNA Breaks; DNA Damage; DNA Repair; Dose-Response Relationship, Drug; Humans; Models, Biological; Naphthoquinones / pharmacology; Poly (ADP-Ribose) Polymerase-1; Poly(ADP-ribose) Polymerases / metabolism; Reverse Transcriptase Inhibitors / pharmacology; Time Factors
topics (OpenAlex): Cancer therapeutics and mechanisms; Bioactive Compounds and Antitumor Agents; DNA Repair Mechanisms
TL;DR: Insight is given into the mechanism by whichbeta-lap radiosensitizes cancer cells and NHEJ is a potent target for enhancing the therapeutic efficacy of beta-lap alone or in combination with other agents in cancer cells that express elevated NQO1 levels. (via Semantic Scholar)
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3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: June 25, 2021

2006 article

Calcium-dependent Modulation of Poly(ADP-ribose) Polymerase-1 Alters Cellular Metabolism and DNA Repair

Bentle, M. S., Reinicke, K. E., Bey, E. A., Spitz, D. R., & Boothman, D. A. (2006, August 19). Journal of Biological Chemistry.

By: M. Bentle*, K. Reinicke*, E. Bey *, D. Spitz * & D. Boothman *

MeSH headings : Adenosine Triphosphate / metabolism; Calcium / metabolism; Cell Death / drug effects; Chelating Agents / pharmacology; DNA / genetics; DNA Damage / genetics; DNA Repair / genetics; Egtazic Acid / analogs & derivatives; Egtazic Acid / pharmacology; Enzyme Activation / drug effects; Humans; Hydrogen Peroxide / pharmacology; NAD / metabolism; Naphthoquinones / pharmacology; Poly (ADP-Ribose) Polymerase-1; Poly(ADP-ribose) Polymerases / genetics; Poly(ADP-ribose) Polymerases / metabolism; Time Factors
topics (OpenAlex): PARP inhibition in cancer therapy; Cancer therapeutics and mechanisms; Bioactive Compounds and Antitumor Agents
TL;DR: β-lapachone is the first chemotherapeutic agent to elicit a Ca2+-mediated cell death by PARP-1 hyperactivation at clinically relevent doses in cancer cells expressing elevated NAD(P)H:quinone oxidoreductase 1 (NQO1) levels. (via Semantic Scholar)
Source: ORCID
Added: June 25, 2021

2006 article

Mornings with art, lessons learned: Feedback regulation, restriction threshold biology, and redundancy govern molecular stress responses

Bey, E. A., Wuerzberger‐Davis, S. M., Pink, J. J., Yang, C. R., Araki, S., Reinicke, K. E., … Boothman, D. A. (2006, September 25). Journal of Cellular Physiology.

By: E. Bey *, S. Wuerzberger‐Davis*, J. Pink *, C. Yang *, S. Araki*, K. Reinicke*, M. Bentle*, Y. Dong * ...

MeSH headings : Apoptosis / drug effects; Cell Cycle / drug effects; Cell Cycle / physiology; Cell Death / drug effects; Cell Physiological Phenomena; Clusterin / genetics; Clusterin / metabolism; DNA Mismatch Repair; Feedback, Physiological / physiology; Humans; Naphthoquinones / pharmacology; Naphthoquinones / therapeutic use; Neoplasms / drug therapy; Radiation-Sensitizing Agents / pharmacology; Radiation-Sensitizing Agents / therapeutic use
topics (OpenAlex): Clusterin in disease pathology; Mitochondrial Function and Pathology; Cell death mechanisms and regulation
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UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: June 25, 2021

2006 article

New tricks for old drugs: the anticarcinogenic potential of DNA repair inhibitors

Bentle, M. S., Bey, E. A., Dong, Y., Reinicke, K. E., & Boothman, D. A. (2006, July 25). Journal of Molecular Histology.

By: M. Bentle*, E. Bey *, Y. Dong *, K. Reinicke* & D. Boothman *

author keywords: DNA repair inhibitors; carcinogenesis; beta-lapachone; DNA repair; NQ01; poly(ADP-ribose) polymerase-1; DNA damage; cancer therapy; cancer prevention
MeSH headings : Anticarcinogenic Agents / pharmacology; Cell Transformation, Neoplastic / genetics; Cell Transformation, Neoplastic / metabolism; DNA Breaks, Double-Stranded; DNA Breaks, Single-Stranded; DNA Repair; Humans; Models, Biological; Naphthoquinones / pharmacology; Naphthoquinones / toxicity; Neoplasms / drug therapy; Neoplasms / enzymology; Neoplasms / genetics; Neoplasms / prevention & control; Poly(ADP-ribose) Polymerases / genetics
topics (OpenAlex): Cancer therapeutics and mechanisms; Bioactive Compounds and Antitumor Agents; DNA Repair Mechanisms
TL;DR: A novel anti-tumor agent, β-lapachone, is focused on and its potential to block transformation by modulating poly(ADP-ribose) polymerase-1 is explored, which could decrease carcinogenicity and prevent tumorigenesis. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: June 25, 2021

2005 article

Development of β-Lapachone Prodrugs for Therapy Against Human Cancer Cells with Elevated NAD(P)H:Quinone Oxidoreductase 1 Levels

Reinicke, K. E., Bey, E. A., Bentle, M. S., Pink, J. J., Ingalls, S. T., Hoppel, C. L., … Boothman, D. A. (2005, April 15). Clinical Cancer Research.

By: K. Reinicke*, E. Bey *, M. Bentle, J. Pink *, S. Ingalls*, C. Hoppel *, R. Misico *, G. Arzac * ...

MeSH headings : Cell Division / drug effects; Cell Line, Tumor; Cell Survival / drug effects; Chromatography, High Pressure Liquid; Dose-Response Relationship, Drug; Humans; Magnetic Resonance Spectroscopy; NAD(P)H Dehydrogenase (Quinone) / metabolism; Naphthoquinones / chemistry; Naphthoquinones / pharmacology; Neoplasms / drug therapy; Neoplasms / enzymology; Neoplasms / pathology; Prodrugs / chemical synthesis; Prodrugs / chemistry; Prodrugs / pharmacology; Spectrometry, Mass, Electrospray Ionization; Spectrophotometry, Ultraviolet; Structure-Activity Relationship
topics (OpenAlex): Bioactive Compounds and Antitumor Agents; Cancer therapeutics and mechanisms; Synthesis and Biological Evaluation
Source: ORCID
Added: June 25, 2021

Employment

Updated: July 11th, 2023 16:02

2023 - present

North Carolina State University Raleigh, North Carolina, US
Associate Professor Molecular Biomedical Sciences

2019 - 2023

NC State University Raleigh, NC, US
Assistant Professor Molecular Biomedical Sciences

2014 - 2019

High Point University High Point, NC, US
Assistant Professor of Biochemistry Chemistry

2011 - 2014

North Carolina State University Raleigh, NC, US
Teaching Associate Biotechnology Program

2007 - 2011

University of North Carolina at Chapel Hill Chapel Hill, NC, US
Postdoctoral Research Associate Lineberger Comprehensive Cancer Center

Education

Updated: October 25th, 2017 08:35

2001 - 2007

Case Western Reserve University Cleveland, OH, US
PhD Pharmacology

1997 - 2001

University of Toledo Toledo, OH, US
BS Biological Sciences

Funding History

Funding history based on the linked ORCID record. Updated: March 3rd, 2025 13:35

grant January 1, 2025 - December 1, 2028
Interdisciplinary Doctoral Program in Molecular Biotechnology
U.S. Department of Education
grant February 1, 2024 - January 31, 2027
REU Site: Modeling the Dynamics of Biological Systems
Directorate for Biological Sciences
grant January 1, 2022 - January 1, 2024
Educating the Current and Future Bioindustrial Workforce: Molecular Biotechnology, Bioprocessing Fundamentals and Responsible Innovation
Department of Defense
grant July 1, 2019 - June 30, 2022
MRI: Acquisition of a 400 MHz Nuclear Magnetic Resonance (NMR) Spectrometer to Support Undergraduate Research at High Point University
Directorate for Mathematical & Physical Sciences

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