Melissa Christina Srougi
Also known as: Melissa S. Bentle
Works (25)
Updated: August 22nd, 2024 05:29
2024 journal article
Student experiences with a molecular biotechnology course containing an interactive 3D immersive simulation and its impact on motivational beliefs
PLOS ONE, 19(7).
open access via doi.org (publisher)
UN Sustainable Development Goal Categories
4. Quality Education
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 14, 2024
2023 journal article
Design and Implementation of an Accessible and Open-Sourced <i>In Silico</i> Drug Screening Activity for Cancer Drug Discovery
JOURNAL OF CHEMICAL EDUCATION, 100(10), 4125–4130.
author keywords: Graduate Education/Research; Upper-Division Undergraduate; Interdisciplinary/Multidisciplinary; Biochemistry; Chemoinformatics; Computer-Based Learning; Internet/Web-Based Learning; Biotechnology; Drugs/Pharmaceuticals; Molecular Modeling
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: October 16, 2023
2023 article
Innovating Life Sciences Laboratory Training: Molecular Biology Laboratory Education Modules (MBLEMs) as a Model for Advanced Training at Diverse Institutions
JOURNAL OF BIOLOGICAL CHEMISTRY, Vol. 299, pp. S260–S260.
Sources: Web Of Science, NC State University Libraries
Added: September 11, 2023
2021 journal article
Growth mindset interventions improve academic performance but not mindset in biochemistry
Biochemistry and Molecular Biology Education.
author keywords: academic performance; biochemistry; growth mindset; metacognition
MeSH headings : Academic Performance; Attitude; Humans; Learning; Students; Universities
TL;DR:
The results suggest that although students' mindsets did not change as a result of growth mindset interventions, their positive perceptions about learning versus performance did increase and they significantly outperformed students who did not receive interventions on the final cumulative exam that assessed critical thinking skills.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
4. Quality Education
(OpenAlex)
Source: ORCID
Added: July 17, 2021
2021 journal article
Integrating Bioinformatics Tools into Inquiry-based Molecular Biology Laboratory Education Modules
Frontiers in Education, 6.
Contributors: C. Goller n , n , S. Chen n , L. Schenkman n & R. Kelly n
author keywords: bioinformatics; science education; molecular biotechnology; software tools; case studies
TL;DR:
This new paradigm for biology laboratory education that embraces bioinformatics as a critical component of inquiry-based learning for undergraduate and graduate students representing the life sciences, the physical sciences, and engineering is described.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
4. Quality Education
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 23, 2021
2020 article
Using Metacognitive Strategies to Improve Academic Performance in Biochemistry
Miller, H. B., & Srougi, M. C. (2020, July 9).
TL;DR:
Although students’ mindsets did not change as a result of metacognitive interventions, their positive perceptions about learning vs performance did increase and students receiving growth mindset interventions significantly outperformed students who did not receive interventions on the final cumulative exam that assessed critical thinking skills.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
4. Quality Education
(OpenAlex)
Source: ORCID
Added: June 25, 2021
2019 journal article
Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 10(152).
author keywords: Biochemistry; Issue 152; cell adhesion; cellular spreading; oxidative stress; fibronectin; epithelial cells; ATM inhibition; ruffles; stress fibers; cell circularity
MeSH headings : Animals; Cell Adhesion / drug effects; Cell Adhesion / physiology; Cell Count / methods; Cell Culture Techniques / methods; Cell Line; Cell Line, Transformed; Cells, Cultured; Epithelial Cells / drug effects; Epithelial Cells / metabolism; Extracellular Matrix / drug effects; Extracellular Matrix / metabolism; Fibroblasts / drug effects; Fibroblasts / metabolism; Fibronectins / metabolism; Humans; Microscopy, Fluorescence / methods; Morpholines / pharmacology; Oxidative Stress / drug effects; Oxidative Stress / physiology; Pyrones / pharmacology
TL;DR:
A step-wise protocol is described that utilizes an immunofluorescence-based assay to specifically quantify cell adhesion and spreading of immortalized fibroblast cells on fibronectin (FN) in vitro to examine a broad range of biological questions.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: November 18, 2019
2019 book
Molecular Biology Techniques 4th Edition
In Elsevier. https://www.elsevier.com/books/molecular-biology-techniques/carson/978-0-12-815774-9
Source: ORCID
Added: June 25, 2021
2018 journal article
Loss of ATM positively regulates Rac1 activity and cellular migration through oxidative stress
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 508(4), 1155–1161.
author keywords: ATM; Rac1; Reactive oxygen species; Migration
MeSH headings : Animals; Ataxia Telangiectasia Mutated Proteins / metabolism; Cell Movement; Enzyme Activation; HeLa Cells; Humans; Oxidative Stress; Rats; Reactive Oxygen Species / metabolism; rac1 GTP-Binding Protein / metabolism
TL;DR:
It is shown that loss of ATM kinase activity elevates intracellular ROS, leading to Rac1 activation, which drives cytoskeletal rearrangements resulting in increased cellular spreading and motility.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: April 20, 2020
2018 journal article
Student-Designed High-Throughput Assays to Assess Effects of Growth Insults in Budding Yeast.
Journal of Microbiology & Biology Education.
TL;DR:
An open inquiry-based laboratory activity exploiting a small-scale high-throughput screening assay of the budding yeast S. cerevisiae is designed to promote active learning and introduce students to modern data collection and analysis techniques.
(via Semantic Scholar)
Source: ORCID
Added: June 25, 2021
2017 journal article
Peer learning as a tool to strengthen math skills in introductory chemistry laboratories
Chemistry Education Research and Practice, 19(1), 319–330.
UN Sustainable Development Goal Categories
4. Quality Education
(OpenAlex)
Source: ORCID
Added: June 25, 2021
2014 journal article
Making Heads or Tails: Planarian Stem Cells in the Classroom †
Journal of Microbiology & Biology Education, 15(1), 18–25.
TL;DR:
A hands-on introductory laboratory module that introduces students to stem cell biology and can be easily incorporated into existing curricula is developed and implemented and piloted at the high school, community college, and university levels.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
4. Quality Education
(OpenAlex)
14. Life Below Water
(Web of Science)
Sources: ORCID, Crossref, NC State University Libraries
Added: January 19, 2020
2014 journal article
Tumor-selective, futile redox cycle-induced bystander effects elicited by NQO1 bioactivatable radiosensitizing drugs in triple-negative breast cancers.
Antioxidants & Redox Signaling.
MeSH headings : Animals; Bystander Effect / drug effects; Female; Humans; Mice; Mice, Nude; NAD(P)H Dehydrogenase (Quinone) / deficiency; NAD(P)H Dehydrogenase (Quinone) / genetics; NAD(P)H Dehydrogenase (Quinone) / metabolism; Naphthoquinones / pharmacology; Oxidation-Reduction / drug effects; Quinones / pharmacology; Radiation-Sensitizing Agents / pharmacology; Triple Negative Breast Neoplasms / drug therapy; Triple Negative Breast Neoplasms / metabolism; Triple Negative Breast Neoplasms / pathology; Tumor Cells, Cultured; Xenograft Model Antitumor Assays
TL;DR:
Bystander effects elicited by NQO1 bioactivatable drugs (β-lap or deoxynyboquinone [DNQ]) likely contribute to their efficacies, killing NQo1(+) solid cancer cells and eliminating surrounding heterogeneous N QO1(low) cancer cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: June 25, 2021
2013 journal article
Assessment of a novel group-centered testing schema in an upper-level undergraduate molecular biotechnology course
BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION, 41(4), 232–241.
author keywords: biotechnology; problem-based testing; collaborative learning; undergraduate
MeSH headings : Biotechnology / education; Curriculum; Educational Measurement / methods; Faculty; Humans; Learning; Students
TL;DR:
It is suggested that group‐centered, problem‐based learning is a useful model for achievement of student learning outcomes in courses where it would be infeasible to provide feedback on individual critical thinking assignments due to grading volume.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
4. Quality Education
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2013 journal article
Catalase abrogates β-lapachone-induced PARP1 hyperactivation-directed programmed necrosis in NQO1-positive breast cancers.
Molecular Cancer Therapeutics.
MeSH headings : Breast Neoplasms / drug therapy; Breast Neoplasms / genetics; Breast Neoplasms / pathology; Catalase / genetics; Catalase / metabolism; DNA Breaks, Single-Stranded / drug effects; DNA Damage / drug effects; Female; Gene Expression Regulation, Neoplastic / drug effects; Humans; Hydrogen Peroxide / metabolism; NAD(P)H Dehydrogenase (Quinone) / genetics; NAD(P)H Dehydrogenase (Quinone) / metabolism; Naphthoquinones / administration & dosage; Necrosis / genetics; Necrosis / pathology; Poly (ADP-Ribose) Polymerase-1; Poly(ADP-ribose) Polymerases / genetics; Reactive Oxygen Species / metabolism
TL;DR:
Although PARP1 hyperactivation and NQO1 expression were major determinants of β-lapachone–induced lethality, alterations in catalase expression, including treatment with exogenous enzyme, caused marked cytoprotection, andCatalase is an important resistance factor and highlights H2O2 as an obligate ROS for cell death from this agent.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: June 25, 2021
2013 journal article
Inquiry into chemotherapy-induced p53 activation in cancer cells as a model for teaching signal transduction
Biochemistry and Molecular Biology Education, 41(6), 419–432.
author keywords: cancer; laboratory; cell signaling; p53
MeSH headings : Antineoplastic Agents, Phytogenic / pharmacology; Biochemistry / education; Blotting, Western; Cell Line, Tumor; Cell Survival / drug effects; Drug Therapy / methods; Etoposide / pharmacology; Flow Cytometry; Humans; Medical Laboratory Personnel / education; Microscopy, Fluorescence; Neoplasms / metabolism; Neoplasms / pathology; Phosphorylation / drug effects; Problem-Based Learning / methods; Reproducibility of Results; Research / education; Research / instrumentation; Research Design / standards; Research Report / standards; Signal Transduction / drug effects; Students; Teaching / methods; Tumor Suppressor Protein p53 / metabolism; Universities
TL;DR:
A new laboratory‐intensive course that teaches students the theory and techniques used to study cell signaling pathways as they conduct a hypothesis‐driven research project to elucidate the mechanism of breast cancer cell death caused by a cancer chemotherapeutic agent is developed and implemented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018
2011 journal article
The nuclear guanine nucleotide exchange factors Ect2 and Net1 regulate RhoB-mediated cell death after DNA damage.
PloS One.
MeSH headings : Breast Neoplasms / genetics; Breast Neoplasms / metabolism; Breast Neoplasms / pathology; Carcinoma / genetics; Carcinoma / metabolism; Carcinoma / pathology; Cell Death / drug effects; Cell Death / genetics; Cell Line, Transformed; DNA Damage / drug effects; DNA Damage / genetics; Female; Gene Expression Regulation, Neoplastic / drug effects; Guanine Nucleotide Exchange Factors / antagonists & inhibitors; Guanine Nucleotide Exchange Factors / genetics; Guanine Nucleotide Exchange Factors / metabolism; Guanine Nucleotide Exchange Factors / physiology; HeLa Cells; Humans; Oncogene Proteins / antagonists & inhibitors; Oncogene Proteins / genetics; Oncogene Proteins / metabolism; Oncogene Proteins / physiology; Proto-Oncogene Proteins / antagonists & inhibitors; Proto-Oncogene Proteins / genetics; Proto-Oncogene Proteins / metabolism; Proto-Oncogene Proteins / physiology; RNA, Small Interfering / pharmacology; Tumor Cells, Cultured; rhoB GTP-Binding Protein / antagonists & inhibitors; rhoB GTP-Binding Protein / genetics; rhoB GTP-Binding Protein / metabolism; rhoB GTP-Binding Protein / physiology
TL;DR:
A mechanism involving the nuclear GEFs Ect2 and Net1 for activating RhoB after genotoxic stress is suggested, thereby facilitating cell death after treatment with DNA damaging agents.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: June 25, 2021
2011 journal article
The small GTPase RhoA localizes to the nucleus and is activated by Net1 and DNA damage signals.
PloS One.
MeSH headings : Cell Nucleus / drug effects; Cell Nucleus / metabolism; Cells, Cultured; DNA Damage / physiology; Guanine Nucleotide Exchange Factors / metabolism; HeLa Cells; Humans; Monomeric GTP-Binding Proteins / metabolism; Oncogene Proteins / antagonists & inhibitors; Oncogene Proteins / genetics; Oncogene Proteins / metabolism; Oncogene Proteins / physiology; Protein Transport / drug effects; RNA, Small Interfering / pharmacology; Signal Transduction / drug effects; Signal Transduction / physiology; Tissue Distribution / drug effects; rhoA GTP-Binding Protein / metabolism
TL;DR:
These results demonstrate the existence of a functional Net1/RhoA signaling pathway within the nucleus of the cell and implicate them in the DNA damage response.
(via Semantic Scholar)
Source: ORCID
Added: June 25, 2021
2009 journal article
Undressing a cellular corset: septins exposed.
Nature Cell Biology.
MeSH headings : Animals; Cell Movement / physiology; Cell Polarity / physiology; Cell Shape / physiology; Cell Surface Extensions / metabolism; Cell Surface Extensions / ultrastructure; Chemotaxis, Leukocyte / physiology; Cytoskeletal Proteins / metabolism; GTP Phosphohydrolases / metabolism; Humans; Septins; T-Lymphocytes / metabolism; T-Lymphocytes / ultrastructure
TL;DR:
A new study identifies a previously undefined role for septins as cellular 'corsets', modulating cell shape and providing cortical rigidity in T cells that allows for their controlled migration.
(via Semantic Scholar)
Source: ORCID
Added: June 25, 2021
2007 journal article
An NQO1- and PARP-1-mediated cell death pathway induced in non-small-cell lung cancer cells by beta-lapachone.
Proceedings of the National Academy of Sciences of the United States of America.
author keywords: DNA repair inhibitor; non-small-cell lung cancer; NQO1; apoptosis; mu-calpain cell death
MeSH headings : Antineoplastic Agents / pharmacology; Apoptosis / drug effects; Apoptosis / physiology; Carcinoma, Non-Small-Cell Lung / drug therapy; Carcinoma, Non-Small-Cell Lung / enzymology; Carcinoma, Non-Small-Cell Lung / metabolism; Cell Line; Cell Line, Tumor; Humans; Lung Neoplasms / drug therapy; Lung Neoplasms / enzymology; Lung Neoplasms / metabolism; NAD(P)H Dehydrogenase (Quinone) / biosynthesis; NAD(P)H Dehydrogenase (Quinone) / genetics; NAD(P)H Dehydrogenase (Quinone) / physiology; Naphthoquinones / pharmacology; Poly (ADP-Ribose) Polymerase-1; Poly(ADP-ribose) Polymerases / physiology; Signal Transduction / drug effects; Signal Transduction / physiology; Tumor Cells, Cultured
TL;DR:
The data indicate that the most efficacious strategy using β-lapachone in chemotherapy was to deliver the drug in short pulses, greatly reducing cytotoxicity to NQO1− “normal” cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: June 25, 2021
2007 journal article
Nonhomologous end joining is essential for cellular resistance to the novel antitumor agent, beta-lapachone.
Cancer Research.
MeSH headings : Antineoplastic Agents / pharmacology; Cell Line, Tumor; Cell Survival; Comet Assay; DNA Breaks; DNA Damage; DNA Repair; Dose-Response Relationship, Drug; Humans; Models, Biological; Naphthoquinones / pharmacology; Poly (ADP-Ribose) Polymerase-1; Poly(ADP-ribose) Polymerases / metabolism; Reverse Transcriptase Inhibitors / pharmacology; Time Factors
TL;DR:
Insight is given into the mechanism by whichbeta-lap radiosensitizes cancer cells and NHEJ is a potent target for enhancing the therapeutic efficacy of beta-lap alone or in combination with other agents in cancer cells that express elevated NQO1 levels.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: June 25, 2021
2006 journal article
Calcium-dependent modulation of poly(ADP-ribose) polymerase-1 alters cellular metabolism and DNA repair.
The Journal of Biological Chemistry.
MeSH headings : Adenosine Triphosphate / metabolism; Calcium / metabolism; Cell Death / drug effects; Chelating Agents / pharmacology; DNA / genetics; DNA Damage / genetics; DNA Repair / genetics; Egtazic Acid / analogs & derivatives; Egtazic Acid / pharmacology; Enzyme Activation / drug effects; Humans; Hydrogen Peroxide / pharmacology; NAD / metabolism; Naphthoquinones / pharmacology; Poly (ADP-Ribose) Polymerase-1; Poly(ADP-ribose) Polymerases / genetics; Poly(ADP-ribose) Polymerases / metabolism; Time Factors
TL;DR:
β-lapachone is the first chemotherapeutic agent to elicit a Ca2+-mediated cell death by PARP-1 hyperactivation at clinically relevent doses in cancer cells expressing elevated NAD(P)H:quinone oxidoreductase 1 (NQO1) levels.
(via Semantic Scholar)
Source: ORCID
Added: June 25, 2021
2006 journal article
Mornings with Art, lessons learned: feedback regulation, restriction threshold biology, and redundancy govern molecular stress responses.
Journal of Cellular Physiology.
MeSH headings : Apoptosis / drug effects; Cell Cycle / drug effects; Cell Cycle / physiology; Cell Death / drug effects; Cell Physiological Phenomena; Clusterin / genetics; Clusterin / metabolism; DNA Mismatch Repair; Feedback, Physiological / physiology; Humans; Naphthoquinones / pharmacology; Naphthoquinones / therapeutic use; Neoplasms / drug therapy; Radiation-Sensitizing Agents / pharmacology; Radiation-Sensitizing Agents / therapeutic use
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: June 25, 2021
2006 journal article
New tricks for old drugs: the anticarcinogenic potential of DNA repair inhibitors.
Journal of Molecular Histology.
author keywords: DNA repair inhibitors; carcinogenesis; beta-lapachone; DNA repair; NQ01; poly(ADP-ribose) polymerase-1; DNA damage; cancer therapy; cancer prevention
MeSH headings : Anticarcinogenic Agents / pharmacology; Cell Transformation, Neoplastic / genetics; Cell Transformation, Neoplastic / metabolism; DNA Breaks, Double-Stranded; DNA Breaks, Single-Stranded; DNA Repair; Humans; Models, Biological; Naphthoquinones / pharmacology; Naphthoquinones / toxicity; Neoplasms / drug therapy; Neoplasms / enzymology; Neoplasms / genetics; Neoplasms / prevention & control; Poly(ADP-ribose) Polymerases / genetics
TL;DR:
A novel anti-tumor agent, β-lapachone, is focused on and its potential to block transformation by modulating poly(ADP-ribose) polymerase-1 is explored, which could decrease carcinogenicity and prevent tumorigenesis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: June 25, 2021
2005 journal article
Development of beta-lapachone prodrugs for therapy against human cancer cells with elevated NAD(P)H:quinone oxidoreductase 1 levels.
Clinical Cancer Research : an Official Journal of the American Association for Cancer Research.
MeSH headings : Cell Division / drug effects; Cell Line, Tumor; Cell Survival / drug effects; Chromatography, High Pressure Liquid; Dose-Response Relationship, Drug; Humans; Magnetic Resonance Spectroscopy; NAD(P)H Dehydrogenase (Quinone) / metabolism; Naphthoquinones / chemistry; Naphthoquinones / pharmacology; Neoplasms / drug therapy; Neoplasms / enzymology; Neoplasms / pathology; Prodrugs / chemical synthesis; Prodrugs / chemistry; Prodrugs / pharmacology; Spectrometry, Mass, Electrospray Ionization; Spectrophotometry, Ultraviolet; Structure-Activity Relationship
Source: ORCID
Added: June 25, 2021
Employment
Updated: July 11th, 2023 16:02
2023 - present
North Carolina State University Raleigh, North Carolina, US
Associate Professor
Molecular Biomedical Sciences
2019 - 2023
NC State University Raleigh, NC, US
Assistant Professor
Molecular Biomedical Sciences
2014 - 2019
High Point University High Point, NC, US
Assistant Professor of Biochemistry
Chemistry
2011 - 2014
North Carolina State University Raleigh, NC, US
Teaching Associate
Biotechnology Program
2007 - 2011
University of North Carolina at Chapel Hill Chapel Hill, NC, US
Postdoctoral Research Associate
Lineberger Comprehensive Cancer Center
Education
Updated: October 25th, 2017 08:35
2001 - 2007
Case Western Reserve University Cleveland, OH, US
PhD
Pharmacology
1997 - 2001
University of Toledo Toledo, OH, US
BS
Biological Sciences
Funding History
Funding history based on the linked ORCID record. Updated: July 11th, 2023 16:03
grant
February 1, 2024 - January 31, 2027
REU Site: Modeling the Dynamics of Biological Systems
Directorate for Biological Sciences
grant
January 1, 2022 - January 1, 2024
Educating the Current and Future Bioindustrial Workforce: Molecular Biotechnology, Bioprocessing Fundamentals and Responsible Innovation
Department of Defense
grant
July 1, 2019 - June 30, 2022
MRI: Acquisition of a 400 MHz Nuclear Magnetic Resonance (NMR) Spectrometer to Support Undergraduate Research at High Point University
Directorate for Mathematical & Physical Sciences
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