@article{porras_assié_tietjen_violette_kleiner_gruber-vodicka_dubilier_leisch_2023, title={An intranuclear bacterial parasite of deep-sea mussels expresses apoptosis inhibitors acquired from its host}, url={https://doi.org/10.1101/2023.06.11.544495}, DOI={10.1101/2023.06.11.544495}, abstractNote={Abstract}, author={Porras, Miguel Ángel González and Assié, Adrien and Tietjen, Målin and Violette, Marlene and Kleiner, Manuel and Gruber-Vodicka, Harald and Dubilier, Nicole and Leisch, Nikolaus}, year={2023}, month={Jun} } @article{michellod_bien_birgel_violette_kleiner_fearn_zeidler_gruber-vodicka_dubilier_liebeke_2023, title={De novo phytosterol synthesis in animals}, volume={380}, ISSN={["1095-9203"]}, url={https://doi.org/10.1126/science.add7830}, DOI={10.1126/science.add7830}, abstractNote={ Sterols are vital for nearly all eukaryotes. Their distribution differs in plants and animals, with phytosterols commonly found in plants whereas most animals are dominated by cholesterol. We show that sitosterol, a common sterol of plants, is the most abundant sterol in gutless marine annelids. Using multiomics, metabolite imaging, heterologous gene expression, and enzyme assays, we show that these animals synthesize sitosterol de novo using a noncanonical C-24 sterol methyltransferase (C 24 -SMT). This enzyme is essential for sitosterol synthesis in plants, but not known from most bilaterian animals. Our phylogenetic analyses revealed that C 24 -SMTs are present in representatives of at least five animal phyla, indicating that the synthesis of sterols common to plants is more widespread in animals than currently known. }, number={6644}, journal={SCIENCE}, author={Michellod, Dolma and Bien, Tanja and Birgel, Daniel and Violette, Marlene and Kleiner, Manuel and Fearn, Sarah and Zeidler, Caroline and Gruber-Vodicka, Harald R. and Dubilier, Nicole and Liebeke, Manuel}, year={2023}, month={May}, pages={520–526} } @article{kleiner_kouris_violette_d'angelo_liu_korenek_tolic_sachsenberg_mccalder_lipton_et al._2023, title={Ultra-sensitive isotope probing to quantify activity and substrate assimilation in microbiomes}, volume={11}, ISSN={["2049-2618"]}, DOI={10.1186/s40168-022-01454-1}, abstractNote={Abstract}, number={1}, journal={MICROBIOME}, author={Kleiner, Manuel and Kouris, Angela and Violette, Marlene and D'Angelo, Grace and Liu, Yihua and Korenek, Abigail and Tolic, Nikola and Sachsenberg, Timo and McCalder, Janine and Lipton, Mary S. S. and et al.}, year={2023}, month={Feb} } @article{michellod_bien_birgel_jensen_kleiner_fearn_zeidler_gruber-vodicka_dubilier_liebeke_2022, title={De novo phytosterol synthesis in animals}, volume={4}, url={https://doi.org/10.1101/2022.04.22.489198}, DOI={10.1101/2022.04.22.489198}, abstractNote={Abstract}, publisher={Cold Spring Harbor Laboratory}, author={Michellod, Dolma and Bien, Tanja and Birgel, Daniel and Jensen, Marlene and Kleiner, Manuel and Fearn, Sarah and Zeidler, Caroline and Gruber-Vodicka, Harald R and Dubilier, Nicole and Liebeke, Manuel}, year={2022}, month={Apr} } @article{bossche_kunath_schallert_schaepe_abraham_armengaud_arntzen_bassignani_benndorf_fuchs_et al._2021, title={Critical Assessment of MetaProteome Investigation (CAMPI): a multi-laboratory comparison of established workflows}, volume={12}, ISSN={["2041-1723"]}, DOI={10.1038/s41467-021-27542-8}, abstractNote={Abstract}, number={1}, journal={NATURE COMMUNICATIONS}, author={Bossche, Tim and Kunath, Benoit J. and Schallert, Kay and Schaepe, Stephanie S. and Abraham, Paul E. and Armengaud, Jean and Arntzen, Magnus O. and Bassignani, Ariane and Benndorf, Dirk and Fuchs, Stephan and et al.}, year={2021}, month={Dec} } @article{jensen_wippler_kleiner_2021, title={Evaluation of RNAlater as a Field-Compatible Preservation Method for Metaproteomic Analyses of Bacterium-Animal Symbioses}, volume={9}, ISSN={["2165-0497"]}, url={https://doi.org/10.1128/Spectrum.01429-21}, DOI={10.1128/Spectrum.01429-21}, abstractNote={Metaproteomics, the large-scale identification and quantification of proteins from microbial communities, provide direct insights into the phenotypes of microorganisms on the molecular level. To ensure the integrity of the metaproteomic data, samples need to be preserved immediately after sampling to avoid changes in protein abundance patterns.}, number={2}, journal={MICROBIOLOGY SPECTRUM}, publisher={American Society for Microbiology}, author={Jensen, Marlene and Wippler, Juliane and Kleiner, Manuel}, editor={Gralnick, Jeffrey A.Editor}, year={2021}, month={Oct} } @article{jensen_wippler_kleiner_2021, title={Evaluation of RNAlater™ as a field-compatible preservation method for metaproteomic analyses of bacteria-animal symbioses}, volume={6}, url={https://doi.org/10.1101/2021.06.16.448770}, DOI={10.1101/2021.06.16.448770}, abstractNote={Abstract}, publisher={Cold Spring Harbor Laboratory}, author={Jensen, Marlene and Wippler, Juliane and Kleiner, Manuel}, year={2021}, month={Jun} } @article{kleiner_kouris_jensen_grace_liu_korenek_tolić_sachsenberg_mccalder_lipton_et al._2021, title={Ultra-sensitive isotope probing to quantify activity and substrate assimilation in microbiomes}, volume={3}, url={https://doi.org/10.1101/2021.03.29.437612}, DOI={10.1101/2021.03.29.437612}, abstractNote={Abstract}, publisher={Cold Spring Harbor Laboratory}, author={Kleiner, Manuel and Kouris, Angela and Jensen, Marlene and Grace, D’Angelo and Liu, Yihua and Korenek, Abigail and Tolić, Nikola and Sachsenberg, Timo and McCalder, Janine and Lipton, Mary S. and et al.}, year={2021}, month={Mar} }