Melissa Kay Regan Scheible

Works (8)

Updated: April 20th, 2024 05:00

2024 journal article

The development of non-destructive sampling methods of parchment skins for genetic species identification

PLOS ONE, 19(3).

By: M. Scheible n, T. Stinson n , M. Breen n , B. Callahan n , R. Thomas n & K. Meiklejohn n 

Ed(s): A. Shakoori

TL;DR: A workflow for enriching the mitochondrial genome (mtGenome), generating next-generation sequencing reads to permit species identification, and providing interpretation guidance is defined, defining a workflow for enriching the mitochondrial genome, generating next-generation sequencing reads to permit species identification, and providing interpretation guidance. (via Semantic Scholar)
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15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: March 22, 2024

2023 article

Using FastID to analyze complex SNP mixtures from indoor dust

Meiklejohn, K. A., Scheible, M. K. R., Boggs, L. M., Dunn, R. R., & Ricke, D. O. (2023, April 3). JOURNAL OF FORENSIC SCIENCES, Vol. 4.

By: K. Meiklejohn n , M. Scheible n, L. Boggs n, R. Dunn n  & D. Ricke *

author keywords: FastID; indoor dust; interpretation of DNA mixtures; investigative leads; massively parallel sequencing; MPS; single nucleotide polymorphisms; SNPs
MeSH headings : Humans; Polymorphism, Single Nucleotide; DNA Fingerprinting / methods; Genotype; DNA / analysis; Software; High-Throughput Nucleotide Sequencing / methods; Sequence Analysis, DNA; Microsatellite Repeats
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: April 3, 2023

2021 journal article

An alternate workflow for preparing Precision ID Ancestry and Identity Panel libraries for Illumina sequencing

INTERNATIONAL JOURNAL OF LEGAL MEDICINE, 135(5), 1717–1726.

By: M. Scheible n, E. Timpano n, L. Boggs n & K. Meiklejohn n 

author keywords: Single  nucleotide polymorphisms; Precision ID; Next-generation sequencing; Ancestry SNPs; Identity SNPs
MeSH headings : Female; Gene Library; Genotyping Techniques; High-Throughput Nucleotide Sequencing / methods; Humans; Male; Polymorphism, Single Nucleotide; Sequence Analysis, DNA / methods; Workflow
TL;DR: An alternative workflow to genotype these SNP panels using Illumina chemistry is developed and 93.9% of all SNPs were successfully genotyped across sample types. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: March 5, 2021

2020 journal article

Optimization of the second internal transcribed spacer (ITS2) for characterizing land plants from soil

PLOS ONE, 15(4).

By: E. Timpano n, M. Scheible n & K. Meiklejohn n 

Ed(s): R. Kalendar

MeSH headings : Bryophyta / classification; Bryophyta / genetics; DNA Barcoding, Taxonomic / methods; DNA Barcoding, Taxonomic / standards; DNA, Intergenic / chemistry; DNA, Intergenic / genetics; DNA, Plant / chemistry; DNA, Plant / genetics; Ferns / classification; Ferns / genetics; Magnoliopsida / classification; Magnoliopsida / genetics; Metagenomics / methods; Metagenomics / standards; Polymerase Chain Reaction / methods; Polymerase Chain Reaction / standards; Soil / chemistry
TL;DR: The broad recoverability across land plants is determined when using published ITS2 primer pairs, and the PCR reaction constituents and cycling conditions for the best two performing primer pairs (ITS2F/ITSp4 and ITSp3/ITSu4) are optimized. (via Semantic Scholar)
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15. Life on Land (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: June 15, 2020

2018 journal article

Paternity testing using massively parallel sequencing and the PowerSeq (TM) AUTO/Y system for short tandem repeat sequencing

ELECTROPHORESIS, 39(21), 2669–2673.

By: D. Silva  n, F. Sawitzki*, M. Scheible n, S. Bailey  n, C. Alho * & S. Faith n

author keywords: Massively parallel sequencing; Paternity testing; PowerSeq (TM) AUTO/Y system; Short tandem repeat
MeSH headings : Alleles; Brazil; DNA / genetics; DNA Fingerprinting / methods; Female; High-Throughput Nucleotide Sequencing / methods; Humans; Male; Microsatellite Repeats; Paternity; Sequence Analysis, DNA / methods
TL;DR: Advantages of implementing sequence‐based analysis, MPS, in paternity testing with improved statistical calculations and a greater resolution for the trios/families tested are shown. (via Semantic Scholar)
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Source: Web Of Science
Added: December 3, 2018

2017 journal article

Demonstration of a basic mitochondrial enrichment method to produce the complete mitochondrial genome sequence of the endangered North Atlantic right whale (Eubalaena glacialis)

CONSERVATION GENETICS RESOURCES, 10(3), 483–486.

By: J. Allwood n, M. Scheible n & S. Faith  n

author keywords: Mitochondrial enrichment; Eubalaena glacialis; Mitochondrial genome; Next generation sequencing; Bioinformatics
TL;DR: This method simultaneously allows for efficient sequencing of the mitochondrial genome, while demonstrating quality sequence coverage that is even across the genome, which illustrates a marked improvement in sequence depth compared to that achieved when performing whole genome sequencing using a standard DNA extraction. (via Semantic Scholar)
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14. Life Below Water (OpenAlex)
Source: Web Of Science
Added: October 19, 2018

2017 journal article

Genetic analysis of Southern Brazil subjects using the PowerSeq (TM) AUTO/Y system for short tandem repeat sequencing

FORENSIC SCIENCE INTERNATIONAL-GENETICS, 33, 129–135.

By: D. Silva  n, F. Sawitzki*, M. Scheible n, S. Bailey  n, C. Alho * & S. Faith  n

author keywords: Short tandem repeat; PowerSeq (TM) AUTO/Y system; South Brazil; Sequence variation
MeSH headings : Brazil; Chromosomes, Human, Y; DNA Fingerprinting; Female; Gene Frequency; Genetic Variation; Genetics, Population; High-Throughput Nucleotide Sequencing; Humans; Male; Microsatellite Repeats; Polymerase Chain Reaction; Sequence Analysis, DNA
TL;DR: The results obtained in this study demonstrate the importance of genetic analysis based on sequencing and highlight the diversity of the South Brazilian population when characterized by STR sequencing. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2016 journal article

The mitochondrial landscape of African Americans: An examination of more than 2500 control region haplotypes from 22 US locations

FORENSIC SCIENCE INTERNATIONAL-GENETICS, 22, 139–148.

By: M. Scheible*, R. Just, K. Sturk-Andreaggi, J. Saunier, W. Parson *, T. Parsons *, M. Coble, J. Irwin

author keywords: Mitochondrial DNA; Control region; African American
MeSH headings : Black or African American / genetics; DNA, Mitochondrial / genetics; Databases, Genetic; Forensic Genetics / methods; Genetic Variation; Genetics, Population; Haplotypes; Humans; Mitochondria / genetics; United States
TL;DR: The mitochondrial DNA control region was Sanger-sequenced for a total of 2563 self-identified African Americans, using automated processing techniques and data review standards exceeding guidelines for forensic applications and are suitable for use in a forensic mtDNA database. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

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