Ming Yao

Works (3)

Updated: April 23rd, 2025 19:21

2023 article

Assessing MTT and sulforhodamine B cell proliferation assays under multiple oxygen environments

Yao, M., Walker, G., & Gamcsik, M. P. (2023, July 5). Cytotechnology.

By: M. Yao n, G. Walker n & M. Gamcsik n

author keywords: MTT assay; SRB; Oxygen partial pressure; Hypoxia; Proliferation; Cancer
topics (OpenAlex): Nitric Oxide and Endothelin Effects; Renin-Angiotensin System Studies; Eicosanoids and Hypertension Pharmacology
TL;DR: It is found that the MTT readings do not correlate with cell number for the Caco-2 and PANC-1 cell lines under different oxygen conditions, whereas the sulforhodamine B protein assays perform well under all conditions. (via Semantic Scholar)
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Added: July 24, 2023

2019 article

Flow-Encoded Oxygen Control to Track the Time-Dependence of Molecular Changes Induced by Static or Cycling Hypoxia

Yao, M., Rabbani, Z. N., Sattler, T., Nguyen, K. G., Zaharoff, D. A., Walker, G., & Gamcsik, M. P. (2019, November 7). Analytical Chemistry.

By: M. Yao n, Z. Rabbani n, T. Sattler n, K. Nguyen  n, D. Zaharoff n, G. Walker n, M. Gamcsik n

MeSH headings : Aldehyde Dehydrogenase / metabolism; Caco-2 Cells; Cell Hypoxia; Cell Line, Tumor; Glucose / pharmacokinetics; Humans; Hypoxia-Inducible Factor 1, alpha Subunit / drug effects; Hypoxia-Inducible Factor 1, alpha Subunit / metabolism; Oxygen / metabolism; Oxygen / pharmacology; Pancreatic Neoplasms / pathology; Time Factors
topics (OpenAlex): Cancer, Hypoxia, and Metabolism; Cancer Cells and Metastasis; Neuroblastoma Research and Treatments
TL;DR: A manifold designed to use flow-encoding to produce sequential changes in gas mixtures delivered to a permeable-bottom 96-well plate is fabricated and shown how this manifold and plate design can be used to expose cells to either static or cycling hypoxic conditions for eight different time periods thereby facilitating the study of the time-response of cells to altered oxygen environments. (via Semantic Scholar)
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Added: December 30, 2019

2018 article

Mixing and delivery of multiple controlled oxygen environments to a single multiwell culture plate

Yao, M., Sattler, T., Rabbani, Z. N., Pulliam, T., Walker, G., & Gamcsik, M. P. (2018, September 5). American Journal of Physiology-Cell Physiology.

By: M. Yao n, T. Sattler n, Z. Rabbani n, T. Pulliam  n, G. Walker n & M. Gamcsik n

author keywords: cell culture; cycling hypoxia; finite element analysis; oxygen diffusion; permeability
MeSH headings : Caco-2 Cells; Cell Culture Techniques / instrumentation; Cell Hypoxia / genetics; Cell Proliferation / genetics; Humans; Hypoxia-Inducible Factor 1, alpha Subunit / chemistry; Hypoxia-Inducible Factor 1, alpha Subunit / metabolism; MCF-7 Cells; Oxygen / chemistry; Oxygen / metabolism
topics (OpenAlex): 3D Printing in Biomedical Research; Cancer, Hypoxia, and Metabolism; Hemoglobin structure and function
TL;DR: A 96-well plate-based device that is capable of delivering eight static or dynamically changing oxygen environments to different rows on a single plate that is amenable to multiwell Plate-based molecular assays or drug dose-response studies in static or cycling hypoxia conditions. (via Semantic Scholar)
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Added: December 10, 2018

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