Ming Yao
Works (3)
Updated: November 6th, 2023 07:25
2023 article
Assessing MTT and sulforhodamine B cell proliferation assays under multiple oxygen environments
Yao, M., Walker, G., & Gamcsik, M. P. (2023, July 5). CYTOTECHNOLOGY.
author keywords: MTT assay; SRB; Oxygen partial pressure; Hypoxia; Proliferation; Cancer
TL;DR:
It is found that the MTT readings do not correlate with cell number for the Caco-2 and PANC-1 cell lines under different oxygen conditions, whereas the sulforhodamine B protein assays perform well under all conditions.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: July 24, 2023
2019 journal article
Flow-Encoded Oxygen Control to Track the Time-Dependence of Molecular Changes Induced by Static or Cycling Hypoxia
ANALYTICAL CHEMISTRY, 91(23), 15032–15039.
MeSH headings : Aldehyde Dehydrogenase / metabolism; Caco-2 Cells; Cell Hypoxia; Cell Line, Tumor; Glucose / pharmacokinetics; Humans; Hypoxia-Inducible Factor 1, alpha Subunit / drug effects; Hypoxia-Inducible Factor 1, alpha Subunit / metabolism; Oxygen / metabolism; Oxygen / pharmacology; Pancreatic Neoplasms / pathology; Time Factors
TL;DR:
A manifold designed to use flow-encoding to produce sequential changes in gas mixtures delivered to a permeable-bottom 96-well plate is fabricated and shown how this manifold and plate design can be used to expose cells to either static or cycling hypoxic conditions for eight different time periods thereby facilitating the study of the time-response of cells to altered oxygen environments.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: December 30, 2019
2018 journal article
Mixing and delivery of multiple controlled oxygen environments to a single multiwell culture plate
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 315(5), C766–C775.
author keywords: cell culture; cycling hypoxia; finite element analysis; oxygen diffusion; permeability
MeSH headings : Caco-2 Cells; Cell Culture Techniques / instrumentation; Cell Hypoxia / genetics; Cell Proliferation / genetics; Humans; Hypoxia-Inducible Factor 1, alpha Subunit / chemistry; Hypoxia-Inducible Factor 1, alpha Subunit / metabolism; MCF-7 Cells; Oxygen / chemistry; Oxygen / metabolism
TL;DR:
A 96-well plate-based device that is capable of delivering eight static or dynamically changing oxygen environments to different rows on a single plate that is amenable to multiwell Plate-based molecular assays or drug dose-response studies in static or cycling hypoxia conditions.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: December 10, 2018