@article{bova_tran_balakshin_chen_capanema_naskar_2016, title={An approach towards tailoring interfacial structures and properties of multiphase renewable thermoplastics from lignin-nitrile rubber}, volume={18}, ISSN={["1463-9270"]}, DOI={10.1039/c6gc01067a}, abstractNote={High-performance multiphase thermoplastics were synthesized by reactive mixing of unmodified industrial lignin and low-cost additives in a matrix of general-purpose acrylonitrile-butadiene rubber (NBR).}, number={20}, journal={GREEN CHEMISTRY}, author={Bova, Tony and Tran, Chau D. and Balakshin, Mikhail Y. and Chen, Jihua and Capanema, Ewellyn A. and Naskar, Amit K.}, year={2016}, pages={5423–5437} } @article{balakshin_capanema_santos_chang_jameel_2016, title={Structural analysis of hardwood native lignins by quantitative C-13 NMR spectroscopy}, volume={70}, ISSN={["1437-434X"]}, DOI={10.1515/hf-2014-0328}, abstractNote={Abstract}, number={2}, journal={HOLZFORSCHUNG}, author={Balakshin, Mikhail Yu and Capanema, Ewellyn A. and Santos, Ricardo B. and Chang, Hou-min and Jameel, Hasan}, year={2016}, month={Feb}, pages={95–108} } @article{capanema_balakshin_katahira_chang_jameel_2015, title={HOW WELL DO MWL AND CEL PREPARATIONS REPRESENT THE WHOLE HARDWOOD LIGNIN?}, volume={35}, ISSN={["1532-2319"]}, DOI={10.1080/02773813.2014.892993}, abstractNote={Hardwood lignin preparations were isolated using classical milled wood lignin (MWL) and cellulolytic enzyme lignin (CEL) protocols. Furthermore, we managed to produce a lignin preparation of a very high yield, above 90%, with high purity and minimal structural degradation. This was achieved by dissolution and regeneration of milled wood prior to enzymatic hydrolysis, along with the use of 80% dioxane for lignin extraction. This preparation (RCEL-80) yield was about 4.5 and 2.5 times higher than the yields of the traditional MWL and CEL preparations, correspondingly, at the same milling time. The preparations obtained were comprehensively analyzed with state-of-the-art quantitative NMR techniques and wet chemistry methods. CELs were representative preparations for hardwood lignins as the S/G ratios in the CELs were equivalent to those in lignin in situ. Degradation of the main lignin subunits was very low. Importantly, the structures of CELs were independent of the preparation yield and were very similar to the structure of the high-yield lignin, RCEL-80. In contrast, the structures of MWLs were noticeably dependent on the preparation yield, especially when the yield was below 15–20% of total wood lignin. In particular, the S/G ratio increased with increasing MWL yield, but was still lower than the S/G ratios of the whole lignins in situ, even at high MWL yields. The amounts of β-O-4 lignin units in MWL were lower than those in the corresponding CEL preparations. It has been concluded that CEL preparations were representative of the whole wood lignin whereas MWLs represent a fraction with a lower S/G ratio.}, number={1}, journal={JOURNAL OF WOOD CHEMISTRY AND TECHNOLOGY}, author={Capanema, Ewellyn and Balakshin, Mikhail and Katahira, Rui and Chang, Hou-min and Jameel, Hasan}, year={2015}, pages={17–26} } @article{santos_capanema_balakshin_chang_jameel_2012, title={Lignin Structural Variation in Hardwood Species}, volume={60}, ISSN={["1520-5118"]}, DOI={10.1021/jf301276a}, abstractNote={A comprehensive lignin structure analysis of ten industrially relevant hardwood species is presented. Milled wood lignin (MWL) was isolated from each species using a modified protocol and all milled wood lignin preparations were analyzed through quantitative (13)C NMR spectroscopy, elemental analysis, methoxyl analysis, sugar analysis, and nitrobenzene oxidation. Nitrobenzene oxidation and ozonation were carried out on extractive-free wood, alkali-extracted wood, milled wood lignin, and alkali-extracted lignin. Milled wood lignin isolated by the modified protocol was found to be representative of the total lignin in alkali-extracted wood. Significant variations in lignin structures, such as syringylpropane/guaiacylpropane ratio (S/G ratio), arylglycerol-β-aryl ether (β-O-4), degree of condensation, and elemental and methoxyl contents, were found among the hardwood species studied. These structural variations among species appear to be correlated to a single factor, the syringyl/guaiacyl ratio. A new method to predict the S/G ratio of total lignin in wood was developed, using a calibration line established by the syringaldehyde/vanillin (S/V) ratio (nitrobenzene oxidation) and the S/G ratio ((13)C NMR) of milled wood lignin (MWL).}, number={19}, journal={JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, author={Santos, Ricardo B. and Capanema, Ewellyn A. and Balakshin, Mikhail Yu and Chang, Hou-min and Jameel, Hasan}, year={2012}, month={May}, pages={4923–4930} } @article{balakshin_capanema_gracz_chang_jameel_2011, title={Quantification of lignin-carbohydrate linkages with high-resolution NMR spectroscopy}, volume={233}, ISSN={["0032-0935"]}, DOI={10.1007/s00425-011-1359-2}, abstractNote={A quantitative approach to characterize lignin-carbohydrate complex (LCC) linkages using a combination of quantitative ¹³C NMR and HSQC 2D NMR techniques has been developed. Crude milled wood lignin (MWLc), LCC extracted from MWLc with acetic acid (LCC-AcOH) and cellulolytic enzyme lignin (CEL) preparations were isolated from loblolly pine (Pinus taeda) and white birch (Betula pendula) woods and characterized using this methodology on a routine 300 MHz NMR spectrometer and on a 950 MHz spectrometer equipped with a cryogenic probe. Structural variations in the pine and birch LCC preparations of different types (MWL, CEL and LCC-AcOH) were elucidated. The use of the high field NMR spectrometer equipped with the cryogenic probe resulted in a remarkable improvement in the resolution of the LCC signals and, therefore, is of primary importance for an accurate quantification of LCC linkages. The preparations investigated showed the presence of different amounts of benzyl ether, γ-ester and phenyl glycoside LCC bonds. Benzyl ester moieties were not detected. Pine LCC-AcOH and birch MWLc preparations were preferable for the analysis of phenyl glycoside and ester LCC linkages in pine and birch, correspondingly, whereas CEL preparations were the best to study benzyl ether LCC structures. The data obtained indicate that pinewood contains higher amounts of benzyl ether LCC linkages, but lower amounts of phenyl glycoside and γ-ester LCC moieties as compared to birch wood.}, number={6}, journal={PLANTA}, author={Balakshin, Mikhail and Capanema, Ewellyn and Gracz, Hanna and Chang, Hou-min and Jameel, Hasan}, year={2011}, month={Jun}, pages={1097–1110} } @article{schmidt_schwartzberg_perera_weber-bargioni_carroll_sarkar_bosneaga_urban_song_balakshin_et al._2009, title={Label-free in situ imaging of lignification in the cell wall of low lignin transgenic Populus trichocarpa}, volume={230}, ISSN={["1432-2048"]}, DOI={10.1007/s00425-009-0963-x}, abstractNote={Chemical imaging by confocal Raman microscopy has been used for the visualization of the cellulose and lignin distribution in wood cell walls. Lignin reduction in wood can be achieved by, for example, transgenic suppression of a monolignol biosynthesis gene encoding 4-coumarate-CoA ligase (4CL). Here, we use confocal Raman microscopy to compare lignification in wild type and lignin-reduced 4CL transgenic Populus trichocarpa stem wood with spatial resolution that is sub-μm. Analyzing the lignin Raman bands in the spectral region between 1,600 and 1,700 cm−1, differences in lignin signal intensity and localization are mapped in situ. Transgenic reduction of lignin is particularly pronounced in the S2 wall layer of fibers, suggesting that such transgenic approach may help overcome cell wall recalcitrance to wood saccharification. Spatial heterogeneity in the lignin composition, in particular with regard to ethylenic residues, is observed in both samples.}, number={3}, journal={PLANTA}, author={Schmidt, M. and Schwartzberg, A. M. and Perera, P. N. and Weber-Bargioni, A. and Carroll, A. and Sarkar, P. and Bosneaga, E. and Urban, J. J. and Song, J. and Balakshin, M. Y. and et al.}, year={2009}, month={Aug}, pages={589–597} } @article{balakshin_capanema_chang_2007, title={MWL fraction with a high concentration of lignin-carbohydrate linkages: Isolation and 2D NMR spectroscopic analysis}, volume={61}, ISSN={["1437-434X"]}, DOI={10.1515/HF.2007.001}, abstractNote={Abstract}, number={1}, journal={HOLZFORSCHUNG}, author={Balakshin, Mikhail Yu. and Capanema, Ewellyn A. and Chang, Hou-min}, year={2007}, pages={1–7} } @article{berlin_balakshin_gilkes_kadla_maximenko_kubo_saddler_2006, title={Inhibition of cellulase, xylanase and beta-glucosidase activities by softwood lignin preparations}, volume={125}, ISSN={["0168-1656"]}, DOI={10.1016/j.jbiotec.2006.02.021}, abstractNote={The conversion of lignocellulosic biomass to fuel ethanol typically involves a disruptive pretreatment process followed by enzyme-catalyzed hydrolysis of the cellulose and hemicellulose components to fermentable sugars. Attempts to improve process economics include protein engineering of cellulases, xylanases and related hydrolases to improve their specific activity or stability. However, it is recognized that enzyme performance is reduced during lignocellulose hydrolysis by interaction with lignin or lignin-carbohydrate complex (LCC), so the selection or engineering of enzymes with reduced lignin interaction offers an alternative means of enzyme improvement. This study examines the inhibition of seven cellulase preparations, three xylanase preparations and a beta-glucosidase preparation by two purified, particulate lignin preparations derived from softwood using an organosolv pretreatment process followed by enzymatic hydrolysis. The two lignin preparations had similar particle sizes and surface areas but differed significantly in other physical properties and in their chemical compositions determined by a 2D correlation HSQC NMR technique and quantitative 13C NMR spectroscopy. The various cellulases differed by up to 3.5-fold in their inhibition by lignin, while the xylanases showed less variability (< or = 1.7-fold). Of all the enzymes tested, beta-glucosidase was least affected by lignin.}, number={2}, journal={JOURNAL OF BIOTECHNOLOGY}, author={Berlin, Alex and Balakshin, Mikhail and Gilkes, Neil and Kadla, John and Maximenko, Vera and Kubo, Satoshi and Saddler, Jack}, year={2006}, month={Sep}, pages={198–209} } @article{capanema_balakshin_chen_gratzl_2006, title={Oxidative ammonolysis of technical lignins. Part 4. Effects of the ammonium hydroxide concentration and pH}, volume={26}, ISSN={["0277-3813"]}, DOI={10.1080/02773810600582350}, abstractNote={Abstract The effects of ammonium hydroxide concentration and pH on the kinetics and reaction mechanism of oxidative ammonolysis of Repap organosolv lignin were studied. The reactions were carried out at 100°C with an oxygen pressure of 8 bar (116 psi) and 0.4–1.6 M [NH4OH] and 9–12.7 pH. The resulting N‐modified lignins were analyzed for elemental composition and methoxyl group content. An increase in ammonium hydroxide concentration increased the rate of nitrogen incorporation, oxygen consumption, CO2 formation, and lignin dissolution. The rate of nitrogen incorporation was 0.5 order with respect to NH4OH concentration. The amount of oxygen consumed, oxygen incorporated into the lignin, CO2 formed, and OMe groups eliminated per mole of nitrogen incorporated decreased with increasing ammonium hydroxide concentration indicating that the increase in [NH4OH] accelerated nitrogen incorporation more than lignin oxidation. The dependence of the rate of nitrogen incorporation on the reaction pH went through a maximum leading to the conclusion that HO− competes with ammonia in reactions with electrophilic lignin centers resulting in interruption of nitrogen incorporation into the lignin.}, number={1}, journal={JOURNAL OF WOOD CHEMISTRY AND TECHNOLOGY}, author={Capanema, EA and Balakshin, MY and Chen, CL and Gratzl, JS}, year={2006}, pages={95–109} } @article{balakshin_capanema_goldfarb_frampton_kadla_2005, title={NMR studies on Fraser fir Abies fraseri (Pursh) Poir. Lignins (vol 59, 488, 2005)}, volume={59}, ISSN={["1437-434X"]}, DOI={10.1515/hf.2005.112}, abstractNote={Abstracting & Indexing}, number={6}, journal={HOLZFORSCHUNG}, author={Balakshin, MY and Capanema, EA and Goldfarb, B and Frampton, J and Kadla, JF}, year={2005}, pages={706–706} } @article{capanema_balakshin_kadla_2005, title={Quantitative characterization of a hardwood milled wood lignin by nuclear magnetic resonance spectroscopy}, volume={53}, ISSN={["1520-5118"]}, DOI={10.1021/jf0515330}, abstractNote={The structure of Eucalyptus grandis milled wood lignin (MWL) was investigated by 2D 1H-13C HSQC, HMQC, and 1H-1H TOCSY correlation NMR techniques and by quantitative 13C NMR as well as by the permanganate oxidation degradation technique. The combination of 2D NMR and quantitative 13C NMR spectroscopy of nonacetylated and acetylated lignin preparations allowed reliable identification and calculation of the amount of different lignin structures. About 85% of side-chain moieties were estimated on the structural level. This information was substantiated by data on the quantity of various functional groups and interunit linkages as a whole. A modified method for calculation of the h:g:s ratio has been suggested and compared with previously suggested approaches. E. grandis MWL has been determined to have an h:g:s ratio of 2:36:62. The amounts of various phenolic/etherified noncondensed/condensed guaiacyl and syringyl moieties were approximately estimated. E. grandis MWL contained approximately 0.60/Ar of beta-O-4 moieties along with small amounts of other structural units such as pino/syringyresinol (0.03/Ar), phenylcoumaran (0.03/Ar), and spirodienone (0.05/Ar). The degree of condensation was estimated at approximately 21%; the main condensed structures are 4-O-5 moieties (approximately 0.09/Ar). The structure of E. grandis MWL was compared with those of other lignin preparations isolated from various hardwoods.}, number={25}, journal={JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, author={Capanema, EA and Balakshin, MY and Kadla, JF}, year={2005}, month={Dec}, pages={9639–9649} } @article{capanema_balakshin_kadla_2004, title={A comprehensive approach for quantitative lignin characterization by NMR spectroscopy}, volume={52}, ISSN={["1520-5118"]}, DOI={10.1021/jf035282b}, abstractNote={A detailed approach for the quantification of different lignin structures in milled wood lignin (MWL) has been suggested using a combination of NMR techniques. 1H-13C heteronuclear multiple quantum coherence and quantitative 13C NMR of nonacetylated and acetylated spruce MWL have been found to have a synergetic effect, resulting in significant progress in the characterization of lignin moieties by NMR. About 80% of side chain moieties, such as different beta-O-4, dibenzodioxocin, phenylcoumaran, pinoresinol, and others, have been identified on the structural level. The presence of appreciable amounts of alpha-O-alkyl and gamma-O-alkyl ethers has been suggested. Although the quantification of various condensed moieties was less precise than for side chain structures, reliable information can be obtained. Comparison of the calculated results with known databases on spruce MWL structure shows that the suggested approach is rather informative and comparable with the information obtained from the combination of various wet chemistry methods. Discrepancies between the results obtained in this study and those previously published are discussed.}, number={7}, journal={JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, author={Capanema, EA and Balakshin, MY and Kadla, JF}, year={2004}, month={Apr}, pages={1850–1860} } @article{capanema_balakshin_chen_2004, title={An improved procedure for isolation of residual lignins from hardwood kraft pulps}, volume={58}, ISSN={["1437-434X"]}, DOI={10.1515/HF.2004.070}, abstractNote={Abstract}, number={5}, journal={HOLZFORSCHUNG}, author={Capanema, EA and Balakshin, MY and Chen, CL}, year={2004}, pages={464–472} } @article{balakshin_capanema_chen_gracz_2003, title={Elucidation of the structures of residual and dissolved pine kraft lignins using an HMQC NMR technique}, volume={51}, ISSN={["1520-5118"]}, DOI={10.1021/jf034372d}, abstractNote={Comparative studies on the structures of residual and dissolved lignins isolated from pine kraft pulp and pulping liquor have been undertaken using the (1)H-(13)C HMQC NMR technique, GPC, and sugar analysis to elucidate the reaction mechanisms in kraft pulping and the lignin reactivity. A modified procedure for the isolation of enzymatic residual lignins has resulted in an appreciable decrease in protein contaminants in the residual lignin preparations (N content < 0.2%). The very high dispersion of HMQC spectra allows identification of different lignin moieties, which signals appear overlapped in 1D (13)C NMR spectra. Elucidation of the role of condensation reactions indicates that an increase in the degree of lignin condensation during pulping results from accumulation of original condensed lignin moieties rather than from the formation of new alkyl-aryl structures. Among aryl-vinyl type moieties, only stilbene structures are accumulated in lignin in appreciable amounts. Benzyl ether lignin-carbohydrate bonds involving primary hydroxyl groups of carbohydrates have been detected in residual and dissolved lignin preparations. Structures of the alpha-hydroxyacid type have been postulated to be among the important lignin degradation products in kraft pulping. The effect of the isolation method on the lignin structure and differences between the residual and dissolved lignins are discussed.}, number={21}, journal={JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, author={Balakshin, MY and Capanema, EA and Chen, CL and Gracz, HS}, year={2003}, month={Oct}, pages={6116–6127} } @article{capanema_balakshin_chen_gratzl_kirkman_2002, title={Oxidative ammonolysis of technical lignins - Part 3. Effect of temperature on the reaction rate}, volume={56}, ISSN={["0018-3830"]}, DOI={10.1515/HF.2002.063}, abstractNote={Summary}, number={4}, journal={HOLZFORSCHUNG}, author={Capanema, EA and Balakshin, MY and Chen, CL and Gratzl, JS and Kirkman, AG}, year={2002}, pages={402–415} } @article{balakshin_chen_gratzl_kirkman_jakob_2001, title={Biobleaching of pulp with dioxygen in laccase-mediator system - effect of variables on the reaction kinetics}, volume={16}, ISSN={["1873-3158"]}, DOI={10.1016/S1381-1177(01)00062-5}, abstractNote={Comparative studies were carried out on the kinetics and mechanism of pulp biobleaching with laccase-mediator system (LMS) with two different mediators, 1-hydroxybenzotriazole (HOBT) and N-hydroxyacetanilide (NHAA). The optimal NHAA and laccase charge was found to be 0.1 mmol and 10 U per gram of pulp with pulp consistency of 10%, at the reaction temperature of 40 °C for 8 h under atmospheric pressure, respectively. The kinetic studies on Kappa number reduction and dioxygen uptake suggest that a very fast rate of delignification with NHAA at the beginning of the process is the result of fast formation of the oxidized mediator species. However, a very slow delignification rate after the initial phase (0.5–1 h) could be caused by low stability of the mediator species. After the reaction time of 2 h, the degree of delignification is higher when HOBT is used as mediator. In contrast to the delignification with NHAA, the formation of the oxidized mediator species is the rate-determining step of the pulp biobleaching with dioxygen in the LMS using HOBT as mediator. Increase in temperature increases the rate of chemical reactions, but decreases the laccase stability. The optimal temperature for pulp biobleaching with HOBT and laccase from Coriolus versicolor is 40 °C. Increasing oxygen pressure improves the efficiency of delignification due to better penetration of the reagents, but does not affect the rate of chemical reactions. The reaction mechanism is discussed based on the kinetic data.}, number={3-4}, journal={JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC}, author={Balakshin, M and Chen, CL and Gratzl, JS and Kirkman, AG and Jakob, H}, year={2001}, month={Dec}, pages={205–215} } @article{balakshin_capanema_chen_gratzl_kirkman_gracz_2001, title={Biobleaching of pulp with dioxygen in the laccase-mediator system - reaction mechanisms for degradation of residual lignin}, volume={13}, ISSN={["1873-3158"]}, DOI={10.1016/S1381-1177(00)00225-3}, abstractNote={Pine Kraft-AQ pulp was biobleached with pressurized dioxygen at 40°C in laccase-mediator system (LMS), i.e. in acetate buffer (pH 4.5) containing Coriolus-laccase and 1-hydroxy-benzotriazole (HOBT), the latter being as a mediator. The LMS-treatment was followed by alkaline extraction (E) under standard conditions. The structures of the residual lignins before and after the biobleaching did not differ appreciably. This indicates that only a part of the residual lignin in the pulp undergoes oxidative degradation in the LMS treatment. In contrast, the treatment resulted in strong changes in the structure of the lignin isolated from E-effluents. The 2D HMQC (1H13C correlation) spectra showed the disappearance of β-O-4′, β-β′ and β-5′ bonds in the structure of the alkaline soluble lignin (ASL) from E-effluents, which are present in the 2D spectrum of the original residual lignin (RKL). In addition, the spectra exhibited new signals that are assigned to ArCOOH in biphenyl (5-5′) moieties. This implies that oxidative cleavage of side chains plays an important role in the delignification of pulp. The NMR studies also indicated that intensive degradation of aromatic ring has occurred in the biobleaching. However, premethylation of neither benzyl alcohol nor phenolic hydroxyl groups of the residual lignin in pulp before the biobleaching affected the rate of delignification. The latter indicates that phenolic moieties participate not only in oxidative degradation but also dehydrogenative polymerization reactions in the biobleaching. This is consistent with an appreciable increase in the proportion of fractions with higher molecular mass in lignin isolated from E-effluents.}, number={1-3}, journal={JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC}, author={Balakshin, M and Capanema, E and Chen, CL and Gratzl, J and Kirkman, A and Gracz, H}, year={2001}, month={Apr}, pages={1–16} } @article{capanema_balakshin_chen_gratzl_kirkman_2001, title={Oxidative ammonolysis of technical lignins - Part 1. Kinetics of the reaction under isothermal condition at 130 degrees C}, volume={55}, ISSN={["0018-3830"]}, DOI={10.1515/HF.2001.066}, abstractNote={Summary}, number={4}, journal={HOLZFORSCHUNG}, author={Capanema, EA and Balakshin, MY and Chen, CL and Gratzl, JS and Kirkman, AG}, year={2001}, pages={397–404} } @article{capanema_balakshin_chen_gratzl_kirkman_2001, title={Oxidative ammonolysis of technical lignins - Part 2. Effect of oxygen pressure}, volume={55}, ISSN={["0018-3830"]}, DOI={10.1515/HF.2001.067}, abstractNote={Summary}, number={4}, journal={HOLZFORSCHUNG}, author={Capanema, EA and Balakshin, MY and Chen, CL and Gratzl, JS and Kirkman, AG}, year={2001}, pages={405–412} } @article{capanema_balakshin_chen_gratzl_gracz_2001, title={Structural analysis of residual and technical lignins by H-1-C-13 correlation 2D NMR-spectroscopy}, volume={55}, ISSN={["1437-434X"]}, DOI={10.1515/HF.2001.050}, abstractNote={Summary}, number={3}, journal={HOLZFORSCHUNG}, author={Capanema, EA and Balakshin, MY and Chen, CL and Gratzl, JS and Gracz, H}, year={2001}, pages={302–308} } @article{balakskin_chen_gratzl_kirkman_jakob_2000, title={Biobleaching of pulp with dioxygen in the laccase-mediator system. Part 1. Kinetics of delignification}, volume={54}, ISSN={["0018-3830"]}, DOI={10.1515/HF.2000.066}, abstractNote={Summary}, number={4}, journal={HOLZFORSCHUNG}, author={Balakskin, M and Chen, CL and Gratzl, JS and Kirkman, AG and Jakob, H}, year={2000}, pages={390–396} } @article{balakshin_chen_gratzl_kirkman_jakob_2000, title={Kinetic studies on oxidation of veratryl alcohol by laccase-mediator system - Part 1. Effects of mediator concentration}, volume={54}, ISSN={["0018-3830"]}, DOI={10.1515/HF.2000.028}, abstractNote={Summary}, number={2}, journal={HOLZFORSCHUNG}, author={Balakshin, MY and Chen, CL and Gratzl, JS and Kirkman, AG and Jakob, H}, year={2000}, pages={165–170} } @article{balakshin_chen_gratzl_kirkman_jakob_2000, title={Kinetic studies on oxidation of veratryl alcohol by laccase-mediator system. Part 2. The kinetics of dioxygen uptake}, volume={54}, ISSN={["0018-3830"]}, DOI={10.1515/HF.2000.029}, abstractNote={Summary}, number={2}, journal={HOLZFORSCHUNG}, author={Balakshin, MY and Chen, CL and Gratzl, JS and Kirkman, AG and Jakob, H}, year={2000}, pages={171–175} }