Evaluation of a commercial microbial enrichment kit used prior DNA extraction to improve the molecular detection of vector-borne pathogens from naturally infected dogs
JOURNAL OF MICROBIOLOGICAL METHODS, 188.
United Kingdom of Great Britain and Northern Ireland 🇬🇧 United States of America 🇺🇸
author keywords: Tick-borne disease; 16S rRNA gene; Next-generation sequencing; Real-time PCR; MolYsis; Anaplasma phagocytophilum; Ehrlichia ewingii; Microbial community; DNA extraction
MeSH headings :
Anaplasma / genetics; Anaplasma phagocytophilum; Animals; Bacteria / genetics; DNA, Bacterial / isolation & purification; DNA, Protozoan / isolation & purification; Dog Diseases / diagnosis; Dogs; Ehrlichia / genetics; High-Throughput Nucleotide Sequencing; Microbiota; Polymerase Chain Reaction; RNA, Ribosomal, 16S / genetics; RNA, Ribosomal, 18S / genetics; Real-Time Polymerase Chain Reaction; Tick-Borne Diseases; Vector Borne Diseases / diagnosis; Vector Borne Diseases / microbiology; Vector Borne Diseases / parasitology
Sources: Web Of Science, ORCID
Added: September 7, 2021