@article{hentz_mosley_2016, title={Development of a stability indicating method for green fluorescent protein by HPLC}, volume={34}, number={10}, journal={LC GC North America}, author={Hentz, N. and Mosley, B.}, year={2016}, pages={798-} }
 @article{stallings_kitchener_hentz_2014, title={A High-Temperature, High-Throughput Method for Monitoring Residual Formaldehyde in Vaccine Formulations}, volume={19}, ISSN={["1540-2452"]}, DOI={10.1177/2211068213504096}, abstractNote={Formaldehyde has long been used in the chemical inactivation of viral material during vaccine production. Viral inactivation is required so that the vaccine does not infect the patient. Formaldehyde is diluted during the vaccine manufacturing process, but residual quantities of formaldehyde are still present in some current vaccines. Although formaldehyde is considered safe for use in vaccines by the Food and Drug Administration, excessive exposure to this chemical may lead to cancer or other health-related issues. An assay was developed that is capable of detecting levels of residual formaldehyde in influenza vaccine samples. The assay employs incubation of dosage formulation suspensions with hydralazine hydrochloride under mildly acidic conditions and elevated temperatures, where formaldehyde is derivatized to yield fluorescent s-triazolo-[3,4-a]-phthalazine. The assay has been traditionally run by high-performance liquid chromatography, where runtimes of 15 minutes per sample can be expected. Our laboratory has developed a plate-based version that drastically improved the throughput to a runtime of 96 samples per minute. The assay was characterized and validated with respect to reaction temperature, evaporation, stability, and selectivity to monitor residual formaldehyde in various influenza vaccine samples, including in-process samples. Heat transfer and evaporation will be especially considered in this work. Since the assay is plate based, it is automation friendly. The new assay format has attained detection limits of 0.01 µg/mL residual formaldehyde, which is easily able to detect and quantify formaldehyde at levels used in many current vaccine formulations (<5 µg/0.5-mL dose).}, number={3}, journal={JALA}, author={Stallings, Kendra D. and Kitchener, Rebecca L. and Hentz, Nathaniel G.}, year={2014}, month={Jun}, pages={275–284} }
 @article{hentz_knaide_2014, title={Effect of liquid-handling accuracy on assay performance}, volume={19}, DOI={10.1177/2211068213504095}, abstractNote={This study illustrates how optimization of both liquid-handling accuracy and precision is critical to assay performance. The study was designed to examine (1) liquid-handling performance and (2) the effect of liquid-handling variability on two types of in vitro biochemical assays by making small but deliberate changes to assay volume delivery. Specifically, protein binding (streptavidin) and enzyme (α-galactosidase) assays were investigated by determining the effect of assay volume for each assay component. The concomitant effect of the liquid-handling variability was then measured via inhibitor potency and assay performance characteristics such as Z-factor, signal-to-background, and variability. It was found that small changes in assay component volumes were indeed measurable by potency (IC50) but not necessarily by assay variability (Z-factor). In fact, this study demonstrates how a miscalibrated liquid handler can lead to erroneous data.}, number={2}, journal={Jala}, author={Hentz, N. G. and Knaide, T. R.}, year={2014}, pages={153–162} }
 @article{krommenhoek_wang_hentz_johnston-peck_kozek_kalyuzhny_tracy_2012, title={Bulky Adamantanethiolate and Cyclohexanethiolate Ligands Favor Smaller Gold Nanoparticles with Altered Discrete Sizes}, volume={6}, ISSN={["1936-0851"]}, url={http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000305661300037&KeyUID=WOS:000305661300037}, DOI={10.1021/nn3003778}, abstractNote={Use of bulky ligands (BLs) in the synthesis of metal nanoparticles (NPs) gives smaller core sizes, sharpens the size distribution, and alters the discrete sizes. For BLs, the highly curved surface of small NPs may facilitate growth, but as the size increases and the surface flattens, NP growth may terminate when the ligand monolayer blocks BLs from transporting metal atoms to the NP core. Batches of thiolate-stabilized Au NPs were synthesized using equimolar amounts of 1-adamantanethiol (AdSH), cyclohexanethiol (CySH), or n-hexanethiol (C6SH). The bulky CyS- and AdS-stabilized NPs have smaller, more monodisperse sizes than the C6S-stabilized NPs. As the bulkiness increases, the near-infrared luminescence intensity increases, which is characteristic of small Au NPs. Four new discrete sizes were measured by MALDI-TOF mass spectrometry, Au(30)(SAd)(18), Au(39)(SAd)(23), Au(65)(SCy)(30), and Au(67)(SCy)(30). No Au(25)(SAd)(18) was observed, which suggests that this structure would be too sterically crowded. Use of BLs may also lead to the discovery of new discrete sizes in other systems.}, number={6}, journal={ACS NANO}, author={Krommenhoek, Peter J. and Wang, Junwei and Hentz, Nathaniel and Johnston-Peck, Aaron C. and Kozek, Krystian A. and Kalyuzhny, Gregory and Tracy, Joseph B.}, year={2012}, month={Jun}, pages={4903–4911} }
 @article{nierobisz_hentz_felts_mozdziak_2010, title={Fiber Phenotype and Coenzyme Q(10) Content in Turkey Skeletal Muscles}, volume={192}, ISSN={["1422-6421"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-78649321430&partnerID=MN8TOARS}, DOI={10.1159/000319550}, abstractNote={Phenotypical differences between muscle fibers are associated with a source of cellular energy. Coenzyme Q<sub>10</sub> (CoQ<sub>10</sub>) is a major component of the mitochondrial oxidative phosphorylation process, and it significantly contributes to the production of cellular energy in the form of ATP. The objective of this study was to determine the relationship between whole-tissue CoQ<sub>10</sub> content, mitochondrial CoQ<sub>10</sub> content, mitochondrial protein, and muscle phenotype in turkeys. Four specialized muscles (anterior latissimus dorsi, ALD; posterior latissimus dorsi, PLD; pectoralis major, PM, and biceps femoris, BF) were evaluated in 9- and 20-week-old turkey toms. The amount of muscle mitochondrial protein was determined using the Bradford assay and CoQ<sub>10</sub> content was measured using HPLC-UV. The amount of mitochondrial protein relative to total protein was significantly lower (p < 0.05) at 9 compared to 20 weeks of age. All ALD fibers stained positive for anti-slow (S35) MyHC antibody. The PLD and PM muscle fibers revealed no staining for slow myosin heavy chain (S35 MyHC), whereas half of BF muscle fibers exhibited staining for S35 MyHC at 9 weeks and 70% at 20 weeks of age. The succinate dehydrogenase (SDH) staining data revealed that SDH significantly increases (p < 0.05) in ALD and BF muscles and significantly decreases (p < 0.05) in PLD and PM muscles with age. The study reveals age-related decreases in mitochondrial CoQ<sub>10</sub> content in muscles with fast/glycolytic profile, and demonstrates that muscles with a slow/oxidative phenotypic profile contain a higher proportion of CoQ<sub>10</sub> than muscles with a fast/glycolytic phenotypic profile.}, number={6}, journal={CELLS TISSUES ORGANS}, author={Nierobisz, L. S. and Hentz, N. G. and Felts, J. V. and Mozdziak, P. E.}, year={2010}, pages={382–394} }