Niranjan Parajuli

Works (1)

Updated: July 31st, 2023 21:14

2011 journal article

A high-throughput screen for directed evolution of aminocoumarin amide synthetases

Analytical Biochemistry, 419(1), 61–66.

By: N. Parajuli n & G. Williams n 

author keywords: Directed evolution; Aminocoumarin; High-throughput screening; Natural products
MeSH headings : Acylation; Amide Synthases / metabolism; Aminocoumarins / chemistry; Aminocoumarins / metabolism; Chromatography, High Pressure Liquid; Cloning, Molecular; Directed Molecular Evolution / methods; Enzyme Activation; Escherichia coli / genetics; Escherichia coli / metabolism; Fluorescence; Fluorometry / methods; High-Throughput Screening Assays / methods; Molecular Structure; Novobiocin / analogs & derivatives; Novobiocin / chemistry; Novobiocin / metabolism; Plasmids / genetics; Plasmids / metabolism; Reproducibility of Results; Substrate Specificity
TL;DR: A fluorimetric high-throughput screen is designed and validated, which can be used to report the activity of mutant amide synthetases toward a broad range of coumarin and acyl donor substrates, and is anticipated to accelerate the creation of amid synthetase mutants with new specificities by directed evolution. (via Semantic Scholar)
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Added: August 6, 2018

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