Obtin Alkhamis

Works (14)

Updated: November 25th, 2024 07:12

2024 article

Determining the Precision of High-Throughput Sequencing and Its Influence on Aptamer Selection

Canoura, J., Alkhamis, O., Byrd, C., Wang, L., Bryant, A., & Xiao, Y. (2024, October 25). ANALYTICAL CHEMISTRY, Vol. 10.

By: J. Canoura n, O. Alkhamis n, C. Byrd n, L. Wang n, A. Bryant n & Y. Xiao n 

Sources: Web Of Science, NC State University Libraries
Added: November 12, 2024

2024 article

Developing Aptamer-Based Colorimetric Opioid Tests

Canoura, J., Alkhamis, O., Venzke, M., Ly, P. T., & Xiao, Y. (2024, March 1). JACS AU, Vol. 3.

By: J. Canoura n, O. Alkhamis n, M. Venzke n, P. Ly n & Y. Xiao n 

author keywords: aptamers; SELEX; opioids; high-throughputsequencing; exonuclease; dye-displacement assay; 3,3'-di(3-sulfopropyl)-4,5,4',5'-dibenzo-9-methyl-thiacarbocyanine; Marquis test
TL;DR: This work develops on-site sensors for morphine and structurally related opioid compounds based on in vitro-selected oligonucleotide affinity reagents known as aptamers that enable the naked-eye identification of specific opioids within seconds and will play an important role in combatting opioid abuse. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: March 18, 2024

2024 article

Examining the Relationship between Aptamer Complexity and Molecular Discrimination of a Low-Epitope Target

Wang, L., Canoura, J., Byrd, C., Nguyen, T., Alkhamis, O., Ly, P., & Xiao, Y. (2024, November 11). ACS CENTRAL SCIENCE, Vol. 11.

By: L. Wang n, J. Canoura n, C. Byrd n, T. Nguyen n, O. Alkhamis n, P. Ly n, Y. Xiao n 

Sources: Web Of Science, NC State University Libraries
Added: November 18, 2024

2024 article

Exploring the Landscape of Aptamers: From Cross-Reactive to Selective to Specific, High-Affinity Receptors for Cocaine

Yang, K., Alkhamis, O., Canoura, J., Bryant, A., Gong, E. M., Barbu, M., … Landry, D. W. (2024, February 13). JACS AU, Vol. 2.

By: K. Yang *, O. Alkhamis n, J. Canoura n, A. Bryant n, E. Gong *, M. Barbu *, S. Taylor *, D. Nikic* ...

author keywords: cocaine; aptamer; specific; high-affinity; cross-reactive; hydrophobic receptor
TL;DR: A series of progressively improved DNA aptamers recognizing cocaine are reported, with the final optimized receptors having low nanomolar affinity and over a thousand-fold selectivity over the initial cross-reactants. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: March 11, 2024

2024 journal article

High-Affinity Aptamers for <i>In Vitro</i> and <i>In Vivo</i> Cocaine Sensing

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 146(5), 3230–3240.

By: O. Alkhamis n, J. Canoura n, Y. Wu *, N. Emmons *, Y. Wang *, K. Honeywell *, K. Plaxco *, T. Kippin *, Y. Xiao n 

TL;DR: High-stringency in vitro selection is performed to generate DNA aptamers that bind cocaine with nanomolar affinity and clinically relevant specificity, thus representing a dramatic improvement over the current-generation, micromolar-affinity, low-specificity cocaine aptamers. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: February 19, 2024

2024 journal article

Nuclease-assisted selection of slow-off rate aptamers

SCIENCE ADVANCES, 10(24).

By: O. Alkhamis n, J. Canoura n, L. Wang n & Y. Xiao n 

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Sources: Web Of Science, NC State University Libraries
Added: July 1, 2024

2024 article

Rapid Nuclease-Assisted Selection of High-Affinity Small-Molecule Aptamers

Wang, L., Alkhamis, O., Canoura, J., Yu, H., & Xiao, Y. (2024, July 23). JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol. 7.

By: L. Wang n, O. Alkhamis n, J. Canoura n, H. Yu * & Y. Xiao n 

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Sources: Web Of Science, NC State University Libraries
Added: August 5, 2024

2023 journal article

Aptamer-Based Fentanyl Detection in Biological Fluids

ANALYTICAL CHEMISTRY, 95(49), 18258–18267.

By: J. Canoura n, Y. Liu n, O. Alkhamis n & Y. Xiao n 

TL;DR: This work utilized library-immobilized SELEX to isolate new aptamers─nucleic acid-based bioreceptors that are well-suited for biosensing that can specifically bind fentanyl under physiological conditions and incorporated one of these into an electrochemical aptamer-based sensor that can rapidly detect fentanyl. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: January 29, 2024

2023 journal article

Comparison of Aptamer Signaling Mechanisms Reveals Disparities in Sensor Response and Strategies to Eliminate False Signals

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 145(22), 12407–12422.

By: O. Alkhamis n, J. Canoura n, C. Willis n, L. Wang n, J. Perry n & Y. Xiao n 

MeSH headings : Aptamers, Nucleotide / chemistry; DNA; DNA, Complementary; Sensitivity and Specificity; SELEX Aptamer Technique / methods; Biosensing Techniques / methods
TL;DR: This work has developed a series of sensors using aptamers for the small-molecule drugs flunixin, fentanyl, and furanyl fentanyl and compares their performance, focusing on their specificity, and demonstrates strategies for improving the sensitivity and specificity of aptamer sensors with the development of a "hybrid beacon". (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: July 19, 2023

2023 article

Using Exonucleases for Aptamer Characterization, Engineering, and Sensing

Alkhamis, O., Canoura, J., Ly, P. T., & Xiao, Y. (2023, June 14). ACCOUNTS OF CHEMICAL RESEARCH, Vol. 6.

By: O. Alkhamis n, J. Canoura n, P. Ly  n & Y. Xiao n 

TL;DR: The serendipitously discovered that the digestion of DNA aptamers by exonucleases is inhibited when an aptamer is bound to a ligand, which served as the foundation for the development of three novel aptamer-related methodologies in the laboratory. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: June 26, 2023

2022 journal article

DNA Aptamer-Cyanine Complexes as Generic Colorimetric Small-Molecule Sensors

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 61(3).

By: O. Alkhamis n, J. Canoura n, K. Bukhryakov *, A. Tarifa *, A. DeCaprio * & Y. Xiao n 

author keywords: aptamers; colorimetric detection; cyanine dyes; dye-displacement; small-molecule targets
TL;DR: A rational approach is demonstrated to develop colorimetric cyanine dye-displacement assays that can be broadly applied to DNA aptamers regardless of their structure, sequence, affinity, or the physicochemical properties of their targets. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: April 18, 2022

2022 article

High-throughput quantitative binding analysis of DNA aptamers using exonucleases

Canoura, J., Alkhamis, O., Liu, Y., Willis, C., & Xiao, Y. (2022, December 30). NUCLEIC ACIDS RESEARCH, Vol. 12.

By: J. Canoura n, O. Alkhamis n, Y. Liu n, C. Willis n & Y. Xiao n 

MeSH headings : Aptamers, Nucleotide / chemical synthesis; Aptamers, Nucleotide / chemistry; Exonucleases; Ligands; Nucleic Acids; SELEX Aptamer Technique / methods; Fentanyl / analysis; Robotics
TL;DR: This work has developed a high-throughput method for accurately quantifying aptamer binding affinity, specificity, and cross-reactivity via the kinetics of aptamer digestion by exonucleases and this approach dramatically accelerates the aptamer characterization process and streamlines sensor development. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: January 17, 2023

2022 article

Near-Infrared Dye-Aptamer Assay for Small Molecule Detection in Complex Specimens

Jin, X., Liu, Y., Alkhamis, O., Canoura, J., Bacon, A., Xu, R., … Xiao, Y. (2022, July 7). ANALYTICAL CHEMISTRY, Vol. 7.

By: X. Jin *, Y. Liu n, O. Alkhamis n, J. Canoura n, A. Bacon n, R. Xu*, F. Fu *, Y. Xiao n 

MeSH headings : Aptamers, Nucleotide / chemistry; Biological Assay; Biosensing Techniques / methods
TL;DR: The utility of dye-aptamer NIR biosensors for high-throughput detection of analytes in clinical specimens is shown and the generality of this assay is demonstrated by detecting three different small-molecule analytes with their respective DNA aptamers at clinically relevant concentrations in serum and urine. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: July 26, 2022

2022 article

Systematic Study of in Vitro Selection Stringency Reveals How To Enrich High-Affinity Aptamers

Alkhamis, O., & Xiao, Y. (2022, December 27). JOURNAL OF THE AMERICAN CHEMICAL SOCIETY.

By: O. Alkhamis n & Y. Xiao n 

MeSH headings : Aptamers, Nucleotide / chemistry; SELEX Aptamer Technique / methods; Ligands
TL;DR: By performing four separate trials of SELEX for the same small-molecule target, it is experimentally proved that the use of excessively high target concentrations promotes enrichment of low-affinity binders while also suppressing the enrichment of high-Affinity aptamers. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: January 17, 2023

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