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Works (49)
Updated: April 5th, 2024 00:59
2018 journal article
Chemoenzymatic lignin valorization: Production of epoxidized pre-polymers using Candida antarctica lipase B
ENZYME AND MICROBIAL TECHNOLOGY, 112, 6–13.
author keywords: Lignin modification; Chemoenzymatic epoxidation; Epoxy resins; Candida antarctica lipase B
MeSH headings : Biocatalysis; Bioengineering; Biofuels; Biomass; Bioreactors; Candida / enzymology; Enzyme Stability; Enzymes, Immobilized / metabolism; Epoxy Compounds / chemistry; Epoxy Compounds / metabolism; Fungal Proteins / metabolism; Hydrogen Peroxide / metabolism; Lignin / chemistry; Lignin / metabolism; Lipase / metabolism; Molecular Structure; Molecular Weight; Polymers / chemistry; Polymers / metabolism; Wood
TL;DR:
An innovative chemoenzymatic catalytic system for functionalizing lignin from Organosolv and Kraft pulping processes to obtain oxirane rings was investigated, and residual enzyme activity indicated transient denaturing and irreversible denaturation due to exposure to hydrogen peroxide.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2018 journal article
Ni/HZSM-5 catalyst preparation by deposition-precipitation. Part 2. Catalytic hydrodeoxygenation reactions of lignin model compounds in organic and aqueous systems
Applied Catalysis A: General, 562, 294–309.
author keywords: Hydrodeoxygenation; Lignin; Depolymerization; HZSM-5; Deposition-Precipitation
publications.aston.ac.uk (repository)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Crossref, ORCID
Added: January 25, 2020
2017 journal article
Ni/HZSM-5 catalyst preparation by deposition-precipitation. Part 1. Effect of nickel loading and preparation conditions on catalyst properties
APPLIED CATALYSIS A-GENERAL, 540, 7–20.
author keywords: Nickel; Zeolites; Deposition-precipitation; Lignin
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Lignin as feedstock for fibers and chemicals
Commercializing Biobased Products: Opportunities, Challenges, Benefits, and Risks, 43, 132–165.
Source: NC State University Libraries
Added: August 6, 2018
2015 journal article
Characterization, performance, and applications of a yeast surface display-based biocatalyst
RSC ADVANCES, 5(25), 19166–19175.
TL;DR:
The lyophilized YSD biocatalyst hydrolytic activity matched or exceeded commercial lipase (Novozym 435) immobilized on acrylic resin at equal catalyst loading, and achieved esterification levels 10–50% that of Novozyme 435.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2015 journal article
Performance in synthetic applications of a yeast surface display-based biocatalyst
RSC ADVANCES, 5(39), 30425–30432.
TL;DR:
The activity of both lipases in a completely hydrophobic environment is a step towards more economical biocatalysis of industrial esterification, and the difference between CalB and M37L in water requirements illustrates a critical parameter for optimization of lipase activity in non-aqueous environments.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Enzymatic processing of renewable glycerol into value-added glycerol carbonate
RSC ADVANCES, 3(40), 18596–18604.
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Potential of ozonolysis as a pretreatment for energy grasses
BIORESOURCE TECHNOLOGY, 148, 242–248.
author keywords: Miscanthus; Saccharum; Optimization; Flow configuration; Enzymatic hydrolysis
MeSH headings : Biofuels; Biomass; Biotechnology / methods; Carbohydrates / analysis; Energy-Generating Resources; Glucose / biosynthesis; Hydrolysis / drug effects; Lignin / metabolism; Ozone / pharmacology; Poaceae / drug effects; Xylans / metabolism
TL;DR:
Results showed that ozonolysis was effective in removing up to 59.9% lignin without cellulose degradation, however, subsequent hydrolysis of pretreated solids with Cellic® CTec2 at 0.06 g/g raw biomass provided glucan conversion lower than untreated samples suggesting enzyme inhibition by lign in degradation products formed during oz onolysis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science; OpenAlex)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2013 journal article
Producing biodiesel from cottonseed oil using Rhizopus oryzae ATCC #34612 whole cell biocatalysts: Culture media and cultivation period optimization
Energy for Sustainable Development, 17(4), 331–336.
author keywords: Rhizopus oryzae; Biodiesel; Cottonseed oil; Whole cell biocatalysts; Fungal catalysis
Sources: Crossref, Web Of Science
Added: August 6, 2018
2013 journal article
Real-time monitoring of high-gravity corn mash fermentation using in situ raman spectroscopy
BIOTECHNOLOGY AND BIOENGINEERING, 110(6), 1654–1662.
author keywords: Raman spectroscopy; fermentation; ethanol; biofuels; chemometrics
MeSH headings : Analysis of Variance; Biofuels / analysis; Biomass; Bioreactors; Ethanol / analysis; Ethanol / metabolism; Fermentation; Glucose / metabolism; Maltose / metabolism; Models, Theoretical; Reproducibility of Results; Spectrum Analysis, Raman / methods; Zea mays / chemistry
TL;DR:
This is the first PLS‐based chemometric modeling of corn mash fermentation under typical industrial conditions, and the first Raman‐based monitoring of a fermentation process with glucose, oligosaccharides and polysaccharides present.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 conference paper
Application of plagiarism screening software in the chemical engineering curriculum
ASEE Annual Conference Proceedings.
Source: NC State University Libraries
Added: August 6, 2018
2005 journal article
Web-based delivery of ChE design projects
Chemical Engineering Education, 39(3), 194–199.
Source: NC State University Libraries
Added: August 6, 2018
2003 journal article
Challenges in learning communication skills in chemical engineering
Communication Education, 52(1), 50–56.
UN Sustainable Development Goal Categories
4. Quality Education
(OpenAlex)
Sources: NC State University Libraries, ORCID
Added: August 6, 2018
2003 conference paper
Instruction and assessment of multidisciplinary teaming skills in senior design
Proceedings of the American Association of Engineering Education, USA.
Source: NC State University Libraries
Added: August 6, 2018
2002 journal article
Extraction of 4-nitrophenol from 1-octanol into aqueous solution in a hollow fiber liquid contactor
JOURNAL OF MEMBRANE SCIENCE, 195(2), 193–202.
author keywords: hollow fiber; membrane transport; supported liquid membrane; pH-swing extraction
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
12. Responsible Consumption and Production
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2002 journal article
Toluene bioconversion to p-hydroxybenzoate by fed-batch cultures of recombinant Pseudomonas putida
BIOTECHNOLOGY AND BIOENGINEERING, 77(3), 340–351.
author keywords: bioconversion; p-hydroxybenzoate; fed-batch fermentation; toluene-4-monooxygenase; protein stability; toluene
MeSH headings : Cell Division / genetics; Fermentation; Genetic Engineering / methods; Industrial Microbiology / methods; Models, Biological; Parabens / metabolism; Pseudomonas putida / genetics; Pseudomonas putida / metabolism; Toluene / metabolism
TL;DR:
Differences in HBA production between the two cultures could be correlated to the levels of specific toluene-4-monooxygenase (T4MO) polypeptides, which catalyzes the rate-limiting step in the pathway.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
2000 conference paper
Membrane biotreatment of VOC-laden air
Proceedings of the Air & Waste Management Association's Annual Conference & Exhibition, 2526–2539.
Source: NC State University Libraries
Added: August 6, 2018
2000 article
Safety and tolerability considerations: tricyclic antidepressants vs. selective serotonin reuptake inhibitors
ACTA PSYCHIATRICA SCANDINAVICA, Vol. 101, pp. 17–25.
author keywords: SSRI; TCA; compliance; discontinuation; antidepressant
MeSH headings : Antidepressive Agents, Tricyclic / adverse effects; Clinical Trials as Topic; Cognition Disorders / chemically induced; Drug Tolerance; Humans; Meta-Analysis as Topic; Selective Serotonin Reuptake Inhibitors / adverse effects
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
1999 journal article
Bioconversion of toluene to p-hydroxybenzoate via the construction and characterization of a recombinant Pseudomonas putida
GREEN CHEMISTRY, 1(3), 143–152.
TL;DR:
Biocatalytic production of p-hydroxybenzoate (HBA) provides improved regiospecificity over Kolbe–Schmitt carboxylation of phenol while achieving significant source reductions in the generation of waste and byproducts.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
1999 patent
Bioreactor process for the continuous removal of organic compounds from a vapor phase process stream
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
1998 journal article
Continuous culture dynamics for aniline metabolism by Pseudomonas sp. CIT1
BIOTECHNOLOGY AND BIOENGINEERING, 58(1), 1–12.
author keywords: Pseudomonas; substrate inhibition; metabolic flux; pathways analysis
TL;DR:
Elucidation of regulatory mechanisms indicates that C23D is noncompetitively inhibited by aniline and subject to feedback inhibition by 2-hydroxymuconic semialdehyde (HMS).
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
1998 journal article
Coordinated clearance of periciliary liquid and mucus from airway surfaces
JOURNAL OF CLINICAL INVESTIGATION, 102(6), 1125–1131.
author keywords: lung; airway; cilia; mucus; mucin; mucociliary clearance; airway surface liquid
MeSH headings : Biological Transport; Body Fluids / metabolism; Cells, Cultured; Epithelial Cells / cytology; Epithelial Cells / physiology; Humans; Models, Biological; Mucociliary Clearance / physiology; Mucus / metabolism; Respiratory Physiological Phenomena
TL;DR:
Mucus-dependent PCL transport was spatially uniform and exceeded the rate expected for pure frictional coupling with the overlying mucus layer; hence, ciliary mixing most likely accelerates the diffusion of momentum from mucus into the PCL.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
1998 journal article
Dynamic modeling of meta- and para-nitrobenzoate metabolism by a mixed co-immobilized culture of Comamonas spp. JS46 and JS47
Biotechnology and Bioengineering, 59(4), 507–516.
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018
1998 journal article
Mobilization of broad host range plasmid from Pseudomonas putida to established biofilm of Bacillus azotoformans. I. Experiments
Biotechnology and Bioengineering, 57(3), 272–279.
TL;DR:
Experimental results demonstrated that the broad host range RSF1010 derivative pDLB101 was transferred to and expressed by B. azotoformans and plasmid transfer was higher between donors and recipients than between transconjugants and recipients.
(via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018
1998 journal article
Mobilization of broad host range plasmid from Pseudomonas putida to established biofilm of Bacillus azotoformans. II. Modeling
Biotechnology and Bioengineering, 57(3), 280–286.
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(Web of Science)
Source: NC State University Libraries
Added: August 6, 2018
1998 journal article
Operation of mixed-culture immobilized cell reactors for the metabolism of meta- and para-nitrobenzoate by Comamonas sp. JS46 and Comamonas sp. JS47
BIOTECHNOLOGY AND BIOENGINEERING, 59(1), 21–27.
author keywords: Comamonas; nitrobenzoates; bioremediation; immobilized mixed culture
TL;DR:
Interactions occurring between the two organisms in the coimmobilized system, mediated by the nitrobenzoate metabolites, are discussed relative to the improved response of this arrangement.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
1994 journal article
EFFECTS OF INDUCER LEVELS ON A RECOMBINANT BACTERIAL BIOFILM FORMATION AND GENE-EXPRESSION
BIOTECHNOLOGY LETTERS, 16(9), 903–908.
TL;DR:
A segregationally stable host-plasmid system, E. coli DH5α (pTKW106), was used to study the effect of induction on the accumulation rate of cells and gene expression in biofilm cultures and maximum β-galactosidase mRNA synthesis rates were reached 36 hours after induction for each IPTG concentration.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1994 journal article
EFFECTS OF MEDIUM CARBON-TO-NITROGEN RATIO ON BIOFILM FORMATION AND PLASMID STABILITY
BIOTECHNOLOGY AND BIOENGINEERING, 44(3), 329–336.
author keywords: BIOFILM FORMATION; ESCHERICHIA COLI; C/N RATIO; PLASMID RETENTION; EXTRACELLULAR POLYSACCHARIDE
TL;DR:
Biofilm formation and plasmid segregational instability in biofilm cultures of Escherichia coli DH5α (pMJR1750) were investigated under different medium‐carbon‐to‐nitrogen (C/N) ratios.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1994 journal article
Stimulation of berberine secretion and growth in cell cultures of Thalictrum minus
Plant Cell Reports, 14(2/3), 131–136.
TL;DR:
Parallel changes in L-phenylalanine ammonia lyase activity with the rate of berberine synthesis and the inverse relationship between cell growth and berberines synthesis imply that berberined synthesis is stress-related in this cell line.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018
1993 article
ACID INHIBITION OF IMMOBILIZED CELLS - QUANTITATIVE COMPARISON OF MODEL AND EXPERIMENT
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, Vol. 39, pp. 401–413.
author keywords: IMMOBILIZED CELLS; ACID INHIBITION; MICROFLUORIMETRY
TL;DR:
A quantitative comparision is offered between a kinetic model for product-inhibited, immobilized cell, metabolism and a high-resolution scanning microfluorimetric characterization of variation of cell growth rates with position in immobilizedcell cultures, indicating a strong potential for scanning micro fluorimetry as a quantitative instrument in immobilize cell studies and for use of free cell kinetics in immobilization cell models.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1993 journal article
Effects of biofilm formation on plasmid segregational stability and expression.
NATO ASI Series, Series E: Applied Sciences, 223, 181–198.
Source: NC State University Libraries
Added: August 6, 2018
1993 journal article
PLASMID RETENTION AND GENE-EXPRESSION IN SUSPENDED AND BIOFILM CULTURES OF RECOMBINANT ESCHERICHIA-COLI DH5 ALPHA(PMJR1750)
BIOTECHNOLOGY AND BIOENGINEERING, 41(2), 211–220.
author keywords: PLASMID RETENTION; GENE EXPRESSION; BIOFILM; BETA-GALACTOSIDASE; SEGREGATIONAL INSTABILITY; ESCHERICHIA-COLI
TL;DR:
An alternative mathematical model is proposed which allows the determination of plasmid loss probability in both suspended batch and continuously fed biofilm cultures of Escherichia coli DH5α(PMJR1750).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1992 journal article
ANALYSIS OF PRODUCTIVITY IN LYSIS-DEFICIENT LAMBDA EXPRESSION SYSTEMS
BIOTECHNOLOGY AND BIOENGINEERING, 40(6), 697–704.
author keywords: LAMBDA PHAGE; LYSOGENY; LYTIC STATE; PARTIAL LYSIS; MULTIPLICITY OF INFECTION; TEMPERATURE INDUCTION; INTRACELLULAR; EXTRACELLULAR PRODUCT
TL;DR:
This study has eliminated partial lysis in the expression phase of λ systems and conducted a detailed comparative analysis of these systems in relation to maximization of cloned gene productivity, suggesting possible further enhancement of productivity with stronger promoters.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1992 journal article
Bacteriophage lambda expression systems for enhanced recombinant protein production
NATO ASI Series, Series E: Applied Sciences, 210, 367–372.
Source: NC State University Libraries
Added: August 6, 2018
1992 journal article
CHARACTERIZATION OF THE MUTANT LYTIC STATE IN LAMBDA EXPRESSION SYSTEMS
BIOTECHNOLOGY AND BIOENGINEERING, 39(4), 369–377.
author keywords: LAMBDA PHAGE; TEMPERATURE-SENSITIVE REPRESSOR; AMBER MUTATIONS; SEGREGATIONAL STABILITY; LYSOGENY; LYTIC STATE; PHAGE HOST INTERACTIONS
TL;DR:
High levels of product are found to be due chiefly to a high‐copy number of λ DNA in the mutant lytic state, and there is partial lysis of the culture even in this suppressor‐free system JM105(NM1070).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1992 journal article
REMOVAL OF P-NITROPHENOL FROM AQUEOUS-SOLUTION BY MEMBRANE-SUPPORTED SOLVENT-EXTRACTION
JOURNAL OF MEMBRANE SCIENCE, 75(3), 277–292.
author keywords: BIOTECHNOLOGY; FIBER MEMBRANES; MICROPOROUS AND POROUS MEMBRANES; MEMBRANE SOLVENT EXTRACTION; WATER TREATMENT
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
12. Responsible Consumption and Production
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1992 journal article
Use of flow cell reactors to quantify biofilm formation kinetics
Biotechnology Techniques, 6(3), 193–198.
TL;DR:
A parallel plate flow cell reactor is introduced and used to evaluate cell adhesion and biofilm formation kinetics for four different bacterial strains of the species, E. coli.
(via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018
1991 patent
Bioreactor process for continuous removal of organic toxicants and other oleophilc solutes from an aqueous process stream
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
1991 journal article
CONSTRUCTION OF A SPECIALIZED-RIBOSOME VECTOR FOR CLONED-GENE EXPRESSION IN ESCHERICHIA-COLI
BIOTECHNOLOGY AND BIOENGINEERING, 38(8), 891–906.
author keywords: RIBOSOME VECTOR; CLONED-GENE EXPRESSION; ESCHERICHIA-COLI
TL;DR:
Using a novel, site‐directed mutagenesis technique, construction of the specialized ribosome vector is outlined, and the results of lacZ expression are presented as transcription of both the cloned‐gene and the specialized‐ribosome locus are induced.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1991 journal article
EFFECT OF CHEMICALLY-INDUCED, CLONED-GENE EXPRESSION ON PROTEIN-SYNTHESIS IN ESCHERICHIA-COLI
BIOTECHNOLOGY AND BIOENGINEERING, 38(4), 397–412.
author keywords: ESCHERICHIA-COLI; PROTEIN SYNTHESIS; METABOLIC LIMITATIONS; CLONED-GENE EXPRESSION; BETA-GALACTOSIDASE
TL;DR:
Stability may play a larger role for β‐galactosidase expression with a strong promoter than seen with β‐lactamase production in the elevated copy number system.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1991 journal article
MICROFLUORIMETRIC ANALYSIS OF SPATIAL AND TEMPORAL PATTERNS OF IMMOBILIZED CELL-GROWTH
BIOTECHNOLOGY AND BIOENGINEERING, 38(4), 340–352.
author keywords: IMMOBILIZED CELLS; ESCHERICHIA-COLI; MICROFLUORIMETRY; DNA STAINING; DNA SYNTHESIS
TL;DR:
High‐resolution microfluorimetric procedures are developed to analyze local variations in both immobilized cell loading and growth rate to enable the influence of mass‐transfer limitations and other immobilization effects on cell distribution and activity to be readily quantified.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1990 journal article
DEPRESSION OF PROTEIN SYNTHETIC CAPACITY DUE TO CLONED-GENE EXPRESSION IN ESCHERICHIA-COLI
BIOTECHNOLOGY AND BIOENGINEERING, 36(9), 865–878.
TL;DR:
The data indicate that a limitation develops within the translational capacity of the cell at high levels of cloned‐gene expression and suggest that strategies designed to enhance recombinant protein expression should include manipulation of translation as well as transcription.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1990 journal article
Enhanced plasmid stability through post-segregational killing of plasmid-free cells
Biotechnology Techniques, 4(1), 39–44.
TL;DR:
In order to develop an extremely stable, inducible host/vector system, the following genetic manipulations were made: a recA mutation was introduced into the chromosome of the host strain, the plasmid selectable marker was changed from ampicillin to kanamycin, and the parB stability locus ofplasmid R1 was added to the plasmsid.
(via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018
1990 journal article
LAMBDA VECTORS FOR STABLE CLONED GENE-EXPRESSION
BIOTECHNOLOGY PROGRESS, 6(4), 277–282.
MeSH headings : Bacteriophage lambda / genetics; Bacteriophage lambda / physiology; Cloning, Molecular; Escherichia coli / genetics; Gene Expression; Genetic Vectors; Lysogeny; Plasmids; beta-Galactosidase / biosynthesis; beta-Galactosidase / genetics
TL;DR:
The bacteriophage λ offers a unique opportunity concurrently to minimize segregational instability in recombinant systems by chromosomal integration of the cloned gene and to achieve highCloned gene expression during an abortive lytic phase by using vectors found to be essentially 100% stable in the lysogenic phase.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1989 journal article
TRANSCRIPTION FROM PLASMID GENES, MACROMOLECULAR STABILITY, AND CELL-SPECIFIC PRODUCTIVITY IN ESCHERICHIA-COLI CARRYING COPY NUMBER MUTANT PLASMIDS
BIOTECHNOLOGY AND BIOENGINEERING, 34(7), 902–908.
TL;DR:
Experiments were performed to evaluate the adaptation of Escherichia coli to plasmid maintenance and cloned gene expression and indicate that cellular biosynthetic capacity may not be limiting productivity in recombinant systems.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
1988 journal article
TRANSIENT-RESPONSE SIMULATIONS OF RECOMBINANT MICROBIAL-POPULATIONS
BIOTECHNOLOGY AND BIOENGINEERING, 32(4), 418–429.
TL;DR:
An asynchronous bacterial population has been approximated using a finite number of “computer” cells, each based on a complex single‐cell model for Escherichia coli, leading to the conclusion that inducible systems give greater productivity than those with fixed promoter strength.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
1987 journal article
SIMULATIONS OF HOST-PLASMID INTERACTIONS IN ESCHERICHIA-COLI - COPY NUMBER, PROMOTER STRENGTH, AND RIBOSOME BINDING-SITE STRENGTH EFFECTS ON METABOLIC-ACTIVITY AND PLASMID GENE-EXPRESSION
BIOTECHNOLOGY AND BIOENGINEERING, 29(3), 316–328.
TL;DR:
Results suggest that cloned‐gene expression is limited by cellular transcription capacity, and parametric studies, conveniently implemented using the computer cell, provide important guidance for future experiments directed at better understanding of host–plasmid interactions and at optimizing recombinant system productivity.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
1986 journal article
MECHANISTICALLY DETAILED MODEL OF CELLULAR-METABOLISM FOR GLUCOSE-LIMITED GROWTH OF ESCHERICHIA-COLI B/R-A
BIOTECHNOLOGY AND BIOENGINEERING, 28(11), 1672–1689.
TL;DR:
This model correctly simulates cell growth, cell composition, and the timing of chromosome synthesis as a function of extracellular substrate concentration for glucose‐limited balanced growth and should provide a useful framework for investigating metabolic perturbations to E. coli.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
1986 journal article
STUDIES OF HOST-PLASMID INTERACTIONS IN RECOMBINANT MICROORGANISMS
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 469, 194–211.
MeSH headings : DNA, Recombinant / metabolism; Escherichia coli / genetics; Escherichia coli / growth & development; Fermentation; Genetic Engineering / methods; Kinetics; Plasmids; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / growth & development; Species Specificity
TL;DR:
Maximum theoretical yield factors based on ATP, glucose, and O2 have been estimated for recombinant Escherichia coli and compared with corresponding estimates for host cells alone, indicating major changes in carbon and energetic stoichiometry in recombinant cells in cases of high cloned gene expression.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
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