@article{ripplinger_crespo_pullin_carnaccini_nelson_trindade_reichelt_pairis-garcia_2024, title={Efficacy of a novel cervical dislocation tool for humane euthanasia of broilers and broiler breeders}, volume={103}, ISSN={["1525-3171"]}, url={https://doi.org/10.1016/j.psj.2024.103449}, DOI={10.1016/j.psj.2024.103449}, abstractNote={Euthanasia is an essential task performed daily on commercial poultry farms around the world to safeguard animal welfare. Manual cervical dislocation (MCD) is the most common euthanasia method but can be challenging to perform given the physical strength required to implement this technique. Therefore, the objective of this study was to evaluate the efficacy of a novel cervical dislocation tool (NCDT) compared to MCD. A total of 60 Ross 308 chickens (6-wk old) and 60 Ross 706 parent stock breeders (21-wk old) were enrolled in the study. Birds were sexed, blocked by body weight, and allocated to 1 of 2 treatments: 1) MCD and 2) NCDT. Immediately following euthanasia application, insensibility, and death were monitored. Once death was confirmed, gross evaluation, radiograph, and macroscopic/microscopic scoring were performed. Both euthanasia methods were 100% effective in achieving insensibility followed by cardiac and respiratory arrest in both age groups. In 6-wk-old broilers, there were no differences in insensibility measures or location and severity of the dislocation site by treatment. The NCDT treatment group showed an increased frequency of fractures located at the tooth-like process that projects from the cranial aspect of the centrum of the axis (dens) but had no impact on bird insensibility. For parent stock, differences in nictitating membrane reflex (NMR) and laceration scores for birds euthanized with NCDT were found and likely associated with additional force exerted with the tool. The NCDT is a promising replacement for MCD and future work should address the development of free and accessible training materials for on-farm use.}, number={3}, journal={POULTRY SCIENCE}, author={Ripplinger, Eliza N. and Crespo, Rocio and Pullin, Allison N. and Carnaccini, Silvia and Nelson, Nathan C. and Trindade, Pedro Henrique Esteves and Reichelt, Sara and Pairis-Garcia, Monique}, year={2024}, month={Mar} }
 @article{pinto_schnepper_trindade_cardoso_fioretto_justulin_zanelli_valente_2024, title={The joint action of yeast eisosomes and membraneless organelles in response to ethanol stress}, volume={10}, ISSN={["2405-8440"]}, DOI={10.1016/j.heliyon.2024.e31561}, abstractNote={Elevated ethanol concentrations in yeast affect the plasma membrane. The plasma membrane in yeast has many lipid-protein complexes, such as Pma1 (MCP), Can1 (MCC), and the eisosome complex. We investigated the response of eisosomes, MCPs, and membraneless structures to ethanol stress. We found a correlation between ethanol stress and proton flux with quick acidification of the medium. Moreover, ethanol stress influences the symporter expression in stressed cells. We also suggest that acute stress from ethanol leads to increases in eisosome size and SG number: we hypothesized that eisosomes may protect APC symporters and accumulate an mRNA decay protein in ethanol-stressed cells. Our findings suggest that the joint action of these factors may provide a protective effect on cells under ethanol stress.}, number={10}, journal={HELIYON}, author={Pinto, Camila Moreira and Schnepper, Amanda Piveta and Trindade, Pedro Henrique Esteves and Cardoso, Luiz Henrique and Fioretto, Matheus Naia and Justulin, Luis Antonio and Zanelli, Cleslei Fernando and Valente, Guilherme Targino}, year={2024}, month={May} }
 @article{fonseca_trindade_pinho_justo_tomacheuski_silva_goncalves_luna_2023, title={Development and Validation of the Unesp-Botucatu Goat Acute Pain Scale}, volume={13}, ISSN={["2076-2615"]}, DOI={10.3390/ani13132136}, abstractNote={We aimed to develop and validate the Unesp-Botucatu goat acute pain scale (UGAPS). Thirty goats (5 negative controls and 25 submitted to orchiectomy) were filmed for 7 min at the time points 24 h before and 2 h, 3 h (1 h after analgesia), and 24 h after orchiectomy. After content validation, according to an ethogram and literature, four blind observers analyzed the videos randomly to score the UGAPS, repeating the same assessment in 30 days. According to the confirmatory factor analysis, the UGAPS is unidimensional. Intra- and interobserver reliability was very good for all raters (Intraclass correlation coefficient ≥85%). Spearman’s correlation between UGAPS versus VAS was 0.85 confirming the criterion validity. Internal consistency was 0.60 for Cronbach’s α Cronbach and 0.67 for McDonald’s ω. The item-total correlation was acceptable for 80% of the items (0.3–0.7). Specificity and sensitivity based on the cut-off point were 99% and 90%, respectively. The scale was responsive and demonstrated construct validity shown by the increase and decrease of scores after surgery pain and analgesia, respectively. The cut-off point for rescue analgesia is ≥3 of 10, with an area under the curve of 95.27%. The UGAPS presents content, criterion, and construct validities, responsiveness, and reliability to assess postoperative pain in castrated goats.}, number={13}, journal={ANIMALS}, author={Fonseca, Mariana Werneck and Trindade, Pedro Henrique Esteves and Pinho, Renata Haddad and Justo, Andre Augusto and Tomacheuski, Rubia Mitalli and Silva, Nuno Emanuel de Oliveira Figueiredo da and Goncalves, Heraldo Cesar and Luna, Stelio Pacca Loureiro}, year={2023}, month={Jul} }
 @article{esteves trindade_lopez-soriano_merenda_tomacheuski_pairis-garcia_2023, title={Effects of assessment method (real-time versus video-recorded) on a validated pain-altered behavior scale used in castrated piglets}, volume={13}, ISSN={["2045-2322"]}, DOI={10.1038/s41598-023-45869-8}, abstractNote={Abstract}, number={1}, journal={SCIENTIFIC REPORTS}, author={Esteves Trindade, Pedro Henrique and Lopez-Soriano, Magdiel and Merenda, Victoria Rocha and Tomacheuski, Rubia Mitalli and Pairis-Garcia, Monique Danielle}, year={2023}, month={Oct} }
 @article{trindade_araujo_luna_2023, title={Weighted pain-related behaviors in pigs undergoing castration based on multilevel logistic regression algorithm}, volume={265}, ISSN={["1872-9045"]}, DOI={10.1016/j.applanim.2023.106002}, abstractNote={Pigs are largely raised for food and translational biomedical research. In several intentional and unintentional situations pigs experience pain. In 2020, the Unesp-Botucatu pig composite acute pain scale (UPAPS) was validated according to the COSMIN guidelines for quick and simple assessment of pain in pigs. Recently, an algorithm has been used to estimate the importance of different behaviors in sheep and horses, however, the statistical weight of each pain-related behavior is still unknown for pigs. Herein, we aimed to investigate whether UPAPS pain-related behaviors in pigs have different statistical weights and to rank their importance. A database from a previous study was used, containing the behavioral records of 45 pigs in the perioperative period of castration. The pigs were filmed between 16 and 24 h before the castration, 3.5 and 4 h after the castration, 1.5–2 h after intramuscular administration of analgesics, and 24 h after the end of the castration. Two experienced observers individually and randomly assessed all videos, without knowing which time-point they were observing (blind analysis). At the end of each video observation, the observers recorded whether they would indicate analgesia, if they considered the pigs were suffering pain, according to their clinical experience, and scored the UPAPS. The intra- and inter-observer reliability were calculated with intraclass correlation coefficient and weighted kappa. A multilevel binomial logistic regression algorithm was constructed to estimate the statistical weights of each UPAPS behavior. The intra- and inter-observer reliability values ranged from 0.71 to 0.88. According to the algorithm, 10 of 19 slope coefficients of the behaviors were significant, evidencing a difference in weight for each pain-related behavior in pigs undergoing castration. The change in posture ‘protecting the affected area’ was the heaviest pain-related behavior based on the algorithm. We conclude that the pain-related behaviors in pigs from the UPAPS have different statistical weights. The algorithm has the potential to indicate the degree of importance of different pain behaviors in pigs undergoing castration and, therefore, to select the most relevant pain behaviors to be targeted when assessing pain in pigs.}, journal={APPLIED ANIMAL BEHAVIOUR SCIENCE}, author={Trindade, Pedro Henrique Esteves and Araujo, Ana Lucelia and Luna, Stelio Pacca Loureira}, year={2023}, month={Aug} }
 @article{zuliani_trindade_costa_2023, title={Welfare assessment of horses and mules used in commercial beef cattle ranches in Brazil}, volume={264}, ISSN={["1872-9045"]}, DOI={10.1016/j.applanim.2023.105964}, abstractNote={This study aimed to identify, among the equine welfare indicators included in two available protocols (Assessment Protocol for Horses and AWIN - Welfare Assessment Protocol for Horses), those suitable to assess the welfare of ranch horses and mules in commercial beef cattle ranch in Brazil and report the welfare status of these animals when kept under such management and working conditions. Twenty-two equine animal-based welfare indicators were evaluated in 84 horses and 51 mules from seven commercial ranches located in the state of Mato Grosso, Brazil. Most of the indicators showed no or little changes from an acceptable standard of animal welfare, defined when the welfare indicators were scored 0 or when a small number of animals were scored 1, for variables scored from 0 to 2. Those indicators which indicated frequent welfare problems (forced approach test, hoof problems, body condition score, integument alterations, lesions at mouth corners, and orbital tightening) were subjected to a principal component analysis (PCA). Differences between ranches were compared by the Kruskal-Wallis test, and between mules and horses, the Mann-Whitney test was used. The first principal component of the PCA indicated that forced approach test and integument alterations were directly associated with each other and inversely associated with body condition score, lesions at mouth corners, hoof condition, and orbital tightening. Ranch 1 showed a higher score on the forced approach test than Ranch 5, which may be related to variations in their handling routines. Mules showed a greater avoidance response during the forced approach test, while horses had a higher incidence of hoof problems, which may be associated with the differences in behavior and anatomy of these animals. We conclude that, except for facial expression indicators, all other animal-based welfare indicators were useful for assessing the welfare of ranch horses and mules, but that they were not sufficient to carry out a complete welfare assessment of these animals, since it lacks proper environmental-based indicators. Thus, we consider this study as a first step towards the development of a specific protocol to assess the welfare of ranch horses and mules. Additionally, our results clearly show that most of the welfare problems identified in this study can be prevented or mitigated by training cowhands on how correctly raise and handle the ranch horses and mules under their care.}, journal={APPLIED ANIMAL BEHAVIOUR SCIENCE}, author={Zuliani, Laura and Trindade, Pedro Henrique Esteves and Costa, Mateus Jose Rodrigues Paranhos}, year={2023}, month={Jul} }