Peter J. Attayek

Works (11)

Updated: July 5th, 2023 15:43

2021 article

Hyperglycemia minimally alters primary self-renewing human colonic epithelial cells while TNFα-promotes severe intestinal epithelial dysfunction

Dutton, J. S., Hinman, S. S., Kim, R., Attayek, P. J., Maurer, M., Sims, C. S., & Allbritton, N. L. (2021, May 14). Integrative Biology.

By: J. Dutton n, S. Hinman *, R. Kim *, P. Attayek n, M. Maurer n, C. Sims*, N. Allbritton*

author keywords: hyperglycemia; glucose; TNF alpha; colon; large intestine; intestinal epithelium; primary tissue
MeSH headings : Colon; Epithelial Cells; Humans; Hyperglycemia; Intestinal Mucosa; Tumor Necrosis Factor-alpha
topics (OpenAlex): Pancreatic and Hepatic Oncology Research; Hyperglycemia and glycemic control in critically ill and hospitalized patients; Cancer Cells and Metastasis; Gut microbiota and health; Diet and metabolism studies; Immune cells in cancer
TL;DR: Results suggest that TNFα acted as the dominant modulator of the epithelium relative to glucose, and that control of inflammation rather than glucose may be key to maintaining intestinal homeostasis. (via Semantic Scholar)
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Source: Web Of Science
Added: July 26, 2021

2020 article

Automated platform for cell selection and separation based on four-dimensional motility and matrix degradation

Nowotarski, H. L., Attayek, P. J., & Allbritton, N. L. (2020, January 1). The Analyst.

By: H. Nowotarski *, P. Attayek n & N. Allbritton n

MeSH headings : Automation; Cell Line, Tumor; Cell Movement; Cell Separation / methods; Collagen / chemistry; Humans; Image Processing, Computer-Assisted; Microscopy, Confocal; Tissue Array Analysis
topics (OpenAlex): Cell Image Analysis Techniques; 3D Printing in Biomedical Research; Microfluidic and Bio-sensing Technologies
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Source: Web Of Science
Added: May 8, 2020

2019 article

An in vitro intestinal platform with a self-sustaining oxygen gradient to study the human gut/microbiome interface

Kim, R., Attayek, P. J., Wang, Y., Furtado, K. L., Tamayo, R., Sims, C. E., & Allbritton, N. L. (2019, September 13). Biofabrication.

By: R. Kim  n, P. Attayek n, Y. Wang *, K. Furtado *, R. Tamayo *, C. Sims *, N. Allbritton n

author keywords: intestine-on-chip; oxygen gradient; microbiome; intestinal crypt
MeSH headings : Bacteria / classification; Bacteria / growth & development; Bacteria / isolation & purification; Bacteria / metabolism; Cell Proliferation; Cells, Cultured; Colon / cytology; Colon / metabolism; Colon / microbiology; Epithelial Cells / cytology; Epithelial Cells / metabolism; Gastrointestinal Microbiome; Humans; In Vitro Techniques / methods; Intestines; Models, Biological; Oxygen / metabolism; Stem Cells / cytology; Stem Cells / metabolism
topics (OpenAlex): Gut microbiota and health; 3D Printing in Biomedical Research; Clinical Nutrition and Gastroenterology; Diet and metabolism studies
TL;DR: This platform provides a simple system to create a physiological oxygen gradient across an intestinal mimic while simultaneously supporting anaerobe co-culture. (via Semantic Scholar)
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Source: Web Of Science
Added: December 16, 2019

2019 article

Molecular transport through primary human small intestinal monolayers by culture on a collagen scaffold with a gradient of chemical cross-linking

Speer, J. E., Gunasekara, D. B., Wang, Y., Fallon, J. K., Attayek, P. J., Smith, P. C., … Allbritton, N. L. (2019, April 27). Journal of Biological Engineering.

By: J. Speer*, D. Gunasekara n, Y. Wang *, J. Fallon *, P. Attayek n, P. Smith *, C. Sims *, N. Allbritton n

author keywords: Intestinal transport; Stiffness; Extracellular matrix; Small intestine
topics (OpenAlex): Cancer Cells and Metastasis; 3D Printing in Biomedical Research; Nanoparticle-Based Drug Delivery
TL;DR: The results of this comparison highlight the need to create in vitro intestinal transport platforms whose characteristics mimic the in vivo lamina propria in order to accurately recapitulate epithelial function. (via Semantic Scholar)
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Source: Web Of Science
Added: May 13, 2019

2017 article

In Vitro Generation of Mouse Colon Crypts

Wang, Y., Gunasekara, D. B., Attayek, P. J., Reed, M. I., DiSalvo, M., Nguyen, D. L., … Allbritton, N. L. (2017, August 29). ACS Biomaterials Science & Engineering.

By: Y. Wang *, D. Gunasekara*, P. Attayek n, M. Reed*, M. DiSalvo  n, D. Nguyen*, J. Dutton n, M. Lebhar * ...

author keywords: intestinal epithelial stem cells; differentiation; intestine-on-a-chip; microfabrication; gradient; tissue mimics
topics (OpenAlex): Cancer Cells and Metastasis; 3D Printing in Biomedical Research; Cellular Mechanics and Interactions
TL;DR: A new approach combining microengineered scaffolds, native chemical gradients, and biophysical cues to control primary epithelium ex vivo can serve as a highly functional and physiologically relevant in vitro tissue model. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2016 article

Automated microraft platform to identify and collect non-adherent cells successfully gene-edited with CRISPR-Cas9

Attayek, P. J., Waugh, J. P., Hunsucker, S. A., Grayeski, P. J., Sims, C. E., Armistead, P. M., & Allbritton, N. L. (2016, December 10). Biosensors and Bioelectronics.

By: P. Attayek n, J. Waugh*, S. Hunsucker *, P. Grayeski*, C. Sims *, P. Armistead *, N. Allbritton n

author keywords: Microraft; Cell array; CRISPR-Cas9; Cytometry; Cell sorting
MeSH headings : Biosensing Techniques / instrumentation; Biosensing Techniques / methods; CRISPR-Cas Systems; Equipment Design; Genes, Reporter; Green Fluorescent Proteins / genetics; Humans; Image Processing, Computer-Assisted; K562 Cells; Leukemia / genetics; Mutation; Splicing Factor U2AF / genetics; Tissue Array Analysis / instrumentation; Tissue Array Analysis / methods; Transfection
topics (OpenAlex): CRISPR and Genetic Engineering; Virus-based gene therapy research; Pluripotent Stem Cells Research
TL;DR: The automated system was used to examine the temporal variation in EGFP expression in cells transfected with CRISPR-Cas9 components for gene editing and successfully gene edited two cell colonies demonstrating the desired mutation. (via Semantic Scholar)
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Added: August 6, 2018

2016 article

Identification and isolation of antigen-specific cytotoxic T lymphocytes with an automated microraft sorting system

Attayek, P. J., Hunsucker, S. A., Sims, C. E., Allbritton, N. L., & Armistead, P. M. (2016, January 1). Integrative Biology.

By: P. Attayek n, S. Hunsucker *, C. Sims *, N. Allbritton n & P. Armistead *

MeSH headings : Antigens, Viral / immunology; CD4-Positive T-Lymphocytes / immunology; CD8-Positive T-Lymphocytes / immunology; Cell Separation; Cells, Cultured; Epitope Mapping / instrumentation; Equipment Design; Equipment Failure Analysis; Humans; Immunoassay / instrumentation; Killer Cells, Natural / immunology; Lab-On-A-Chip Devices; T-Lymphocytes, Cytotoxic / immunology; Tissue Array Analysis / instrumentation
topics (OpenAlex): T-cell and B-cell Immunology; Immunotherapy and Immune Responses; CAR-T cell therapy research
TL;DR: A microraft array methodology is developed that automatically measures the ability of individual T cells to kill a population of target cells and viably sorts specific cells into a 96-well plate for expansion, enabling rapid identification of highly cytotoxic CD8+ T cells. (via Semantic Scholar)
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Added: August 6, 2018

2016 journal article

In vitro polarization of colonoids to create an intestinal stem cell compartment

PLoS One, 11(4).

By: P. Attayek, A. Ahmad, Y. Wang, I. Williamson , C. Sims, S. Magness, N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2015 article

Array-Based Platform To Select, Release, and Capture Epstein–Barr Virus-Infected Cells Based on Intercellular Adhesion

Attayek, P. J., Hunsucker, S. A., Wang, Y., Sims, C. E., Armistead, P. M., & Allbritton, N. L. (2015, November 11). Analytical Chemistry.

By: P. Attayek*, S. Hunsucker *, Y. Wang *, C. Sims *, P. Armistead * & N. Allbritton*

MeSH headings : Cell Adhesion; Cell Separation / instrumentation; Cell Separation / methods; Cell Survival; Dimethylpolysiloxanes / chemistry; Epstein-Barr Virus Nuclear Antigens / genetics; Epstein-Barr Virus Nuclear Antigens / metabolism; Herpesvirus 4, Human / physiology; Humans; K562 Cells; Microarray Analysis / instrumentation; Nylons / chemistry; Particle Size; Single-Cell Analysis / instrumentation; Surface Properties; Tumor Cells, Cultured; Viral Proteins / genetics; Viral Proteins / metabolism
topics (OpenAlex): Microwave Engineering and Waveguides; Plant Virus Research Studies; Viral-associated cancers and disorders
TL;DR: Microraft arrays are a versatile method enabling separation of cells based on complicated and as yet poorly understood cell phenotypes, enabling release and collection of the cells without the need for cell attachment to the microraft surface. (via Semantic Scholar)
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Added: August 6, 2018

2013 article

Isolation and in Vitro Culture of Rare Cancer Stem Cells from Patient-Derived Xenografts of Pancreatic Ductal Adenocarcinoma

Gach, P. C., Attayek, P. J., Herrera, G., Yeh, J. J., & Allbritton, N. L. (2013, July 1). Analytical Chemistry.

By: P. Gach*, P. Attayek n, G. Herrera*, J. Yeh * & N. Allbritton n

MeSH headings : Animals; Carcinoma, Pancreatic Ductal / pathology; Cell Culture Techniques / methods; Cell Line, Tumor; Cell Separation / methods; Cell Survival; Cell Transformation, Neoplastic; Humans; Image Processing, Computer-Assisted; Mice; Neoplastic Stem Cells / pathology; Pancreatic Neoplasms / pathology
topics (OpenAlex): Cancer Cells and Metastasis; Pancreatic function and diabetes; 3D Printing in Biomedical Research
TL;DR: A direct comparison of this technology with fluorescence-activated cell sorting (FACS) demonstrated that micropallet arrays offered enhanced post sorting purity (100), yield (100%), and viability (94-100%) for rare cell isolation. (via Semantic Scholar)
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Added: August 6, 2018

2013 article

Micropallet arrays for the capture, isolation and culture of circulating tumor cells from whole blood of mice engrafted with primary human pancreatic adenocarcinoma

Gach, P. C., Attayek, P. J., Whittlesey, R. L., Yeh, J. J., & Allbritton, N. L. (2013, November 20). Biosensors and Bioelectronics.

By: P. Gach*, P. Attayek n, R. Whittlesey*, J. Yeh * & N. Allbritton n

author keywords: Microfabrication; Surface functionalization; Cell array; Circulating tumor cells; Cell sorting; Pancreatic cancer
MeSH headings : Adenocarcinoma / blood; Adenocarcinoma / pathology; Animals; Biosensing Techniques / instrumentation; Cell Line, Tumor; Cell Separation / instrumentation; Equipment Design; Female; Humans; Mice; Neoplastic Cells, Circulating / pathology; Pancreatic Neoplasms / blood; Pancreatic Neoplasms / pathology; Tissue Array Analysis / instrumentation
topics (OpenAlex): Microfluidic and Bio-sensing Technologies; Cancer Cells and Metastasis; 3D Printing in Biomedical Research
TL;DR: Clinical utility of this technology was shown through the capture, isolation and successful culture of CTCs from the blood of mice engrafted with primary human pancreatic tumors and direct capture and isolation of living tumor cells from blood followed by analysis or culture will be a valuable tool for cancer cell characterization. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

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