Works (11)

Updated: July 5th, 2023 15:43

2021 journal article

Hyperglycemia minimally alters primary self-renewing human colonic epithelial cells while TNF alpha-promotes severe intestinal epithelial dysfunction

INTEGRATIVE BIOLOGY, 13(6), 139–152.

By: J. Dutton n, S. Hinman*, R. Kim*, P. Attayek n, M. Maurer n, C. Sims*, N. Allbritton*

author keywords: hyperglycemia; glucose; TNF alpha; colon; large intestine; intestinal epithelium; primary tissue
MeSH headings : Colon; Epithelial Cells; Humans; Hyperglycemia; Intestinal Mucosa; Tumor Necrosis Factor-alpha
Source: Web Of Science
Added: July 26, 2021

2020 journal article

An in vitro intestinal platform with a self-sustaining oxygen gradient to study the human gut/microbiome interface


author keywords: intestine-on-chip; oxygen gradient; microbiome; intestinal crypt
MeSH headings : Bacteria / classification; Bacteria / growth & development; Bacteria / isolation & purification; Bacteria / metabolism; Cell Proliferation; Cells, Cultured; Colon / cytology; Colon / metabolism; Colon / microbiology; Epithelial Cells / cytology; Epithelial Cells / metabolism; Gastrointestinal Microbiome; Humans; In Vitro Techniques / methods; Intestines; Models, Biological; Oxygen / metabolism; Stem Cells / cytology; Stem Cells / metabolism
Source: Web Of Science
Added: December 16, 2019

2020 journal article

Automated platform for cell selection and separation based on four-dimensional motility and matrix degradation

ANALYST, 145(7), 2731–2742.

By: H. Nowotarski*, P. Attayek n & N. Allbritton n

MeSH headings : Automation; Cell Line, Tumor; Cell Movement; Cell Separation / methods; Collagen / chemistry; Humans; Image Processing, Computer-Assisted; Microscopy, Confocal; Tissue Array Analysis
Source: Web Of Science
Added: May 8, 2020

2019 journal article

Molecular transport through primary human small intestinal monolayers by culture on a collagen scaffold with a gradient of chemical cross-linking


By: J. Speer*, D. Gunasekara n, Y. Wang*, J. Fallon*, P. Attayek n, P. Smith*, C. Sims*, N. Allbritton n

author keywords: Intestinal transport; Stiffness; Extracellular matrix; Small intestine
Source: Web Of Science
Added: May 13, 2019

2017 journal article

Automated microraft platform to identify and collect non-adherent cells successfully gene-edited with CRISPR-Cas9


By: P. Attayek n, J. Waugh*, S. Hunsucker*, P. Grayeski*, C. Sims*, P. Armistead*, N. Allbritton n

author keywords: Microraft; Cell array; CRISPR-Cas9; Cytometry; Cell sorting
MeSH headings : Biosensing Techniques / instrumentation; Biosensing Techniques / methods; CRISPR-Cas Systems; Equipment Design; Genes, Reporter; Green Fluorescent Proteins / genetics; Humans; Image Processing, Computer-Assisted; K562 Cells; Leukemia / genetics; Mutation; Splicing Factor U2AF / genetics; Tissue Array Analysis / instrumentation; Tissue Array Analysis / methods; Transfection
Source: Web Of Science
Added: August 6, 2018

2017 journal article

In Vitro Generation of Mouse Colon Crypts


By: Y. Wang*, D. Gunasekara*, P. Attayek n, M. Reed*, M. DiSalvo n, D. Nguyen*, J. Dutton n, M. Lebhar* ...

author keywords: intestinal epithelial stem cells; differentiation; intestine-on-a-chip; microfabrication; gradient; tissue mimics
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Identification and isolation of antigen-specific cytotoxic T lymphocytes with an automated microraft sorting system

INTEGRATIVE BIOLOGY, 8(12), 1208–1220.

By: P. Attayek n, S. Hunsucker*, C. Sims*, N. Allbritton n & P. Armistead*

MeSH headings : Antigens, Viral / immunology; CD4-Positive T-Lymphocytes / immunology; CD8-Positive T-Lymphocytes / immunology; Cell Separation; Cells, Cultured; Epitope Mapping / instrumentation; Equipment Design; Equipment Failure Analysis; Humans; Immunoassay / instrumentation; Killer Cells, Natural / immunology; Lab-On-A-Chip Devices; T-Lymphocytes, Cytotoxic / immunology; Tissue Array Analysis / instrumentation
Source: Web Of Science
Added: August 6, 2018

2016 journal article

In vitro polarization of colonoids to create an intestinal stem cell compartment

PLoS One, 11(4).

By: P. Attayek, A. Ahmad, Y. Wang, I. Williamson, C. Sims, S. Magness, N. Allbritton

Source: NC State University Libraries
Added: August 6, 2018

2015 journal article

Array-Based Platform To Select, Release, and Capture Epstein-Barr Virus-Infected Cells Based on Intercellular Adhesion

ANALYTICAL CHEMISTRY, 87(24), 12281–12289.

MeSH headings : Cell Adhesion; Cell Separation / instrumentation; Cell Separation / methods; Cell Survival; Dimethylpolysiloxanes / chemistry; Epstein-Barr Virus Nuclear Antigens / genetics; Epstein-Barr Virus Nuclear Antigens / metabolism; Herpesvirus 4, Human / physiology; Humans; K562 Cells; Microarray Analysis / instrumentation; Nylons / chemistry; Particle Size; Single-Cell Analysis / instrumentation; Surface Properties; Tumor Cells, Cultured; Viral Proteins / genetics; Viral Proteins / metabolism
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Micropallet arrays for the capture, isolation and culture of circulating tumor cells from whole blood of mice engrafted with primary human pancreatic adenocarcinoma


By: P. Gach*, P. Attayek n, R. Whittlesey*, J. Yeh* & N. Allbritton n

author keywords: Microfabrication; Surface functionalization; Cell array; Circulating tumor cells; Cell sorting; Pancreatic cancer
MeSH headings : Adenocarcinoma / blood; Adenocarcinoma / pathology; Animals; Biosensing Techniques / instrumentation; Cell Line, Tumor; Cell Separation / instrumentation; Equipment Design; Female; Humans; Mice; Neoplastic Cells, Circulating / pathology; Pancreatic Neoplasms / blood; Pancreatic Neoplasms / pathology; Tissue Array Analysis / instrumentation
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Isolation and in Vitro Culture of Rare Cancer Stem Cells from Patient-Derived Xenografts of Pancreatic Ductal Adenocarcinoma

ANALYTICAL CHEMISTRY, 85(15), 7271–7278.

By: P. Gach*, P. Attayek n, G. Herrera*, J. Yeh* & N. Allbritton n

MeSH headings : Animals; Carcinoma, Pancreatic Ductal / pathology; Cell Culture Techniques / methods; Cell Line, Tumor; Cell Separation / methods; Cell Survival; Cell Transformation, Neoplastic; Humans; Image Processing, Computer-Assisted; Mice; Neoplastic Stem Cells / pathology; Pancreatic Neoplasms / pathology
Source: Web Of Science
Added: August 6, 2018