Works (11)
Updated: July 5th, 2023 15:43
2021 journal article
Hyperglycemia minimally alters primary self-renewing human colonic epithelial cells while TNF alpha-promotes severe intestinal epithelial dysfunction
INTEGRATIVE BIOLOGY, 13(6), 139–152.
author keywords: hyperglycemia; glucose; TNF alpha; colon; large intestine; intestinal epithelium; primary tissue
MeSH headings : Colon; Epithelial Cells; Humans; Hyperglycemia; Intestinal Mucosa; Tumor Necrosis Factor-alpha
TL;DR:
Results suggest that TNFα acted as the dominant modulator of the epithelium relative to glucose, and that control of inflammation rather than glucose may be key to maintaining intestinal homeostasis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: July 26, 2021
2020 journal article
Automated platform for cell selection and separation based on four-dimensional motility and matrix degradation
ANALYST, 145(7), 2731–2742.
MeSH headings : Automation; Cell Line, Tumor; Cell Movement; Cell Separation / methods; Collagen / chemistry; Humans; Image Processing, Computer-Assisted; Microscopy, Confocal; Tissue Array Analysis
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: May 8, 2020
2019 journal article
An in vitro intestinal platform with a self-sustaining oxygen gradient to study the human gut/microbiome interface
BIOFABRICATION, 12(1).
author keywords: intestine-on-chip; oxygen gradient; microbiome; intestinal crypt
MeSH headings : Bacteria / classification; Bacteria / growth & development; Bacteria / isolation & purification; Bacteria / metabolism; Cell Proliferation; Cells, Cultured; Colon / cytology; Colon / metabolism; Colon / microbiology; Epithelial Cells / cytology; Epithelial Cells / metabolism; Gastrointestinal Microbiome; Humans; In Vitro Techniques / methods; Intestines; Models, Biological; Oxygen / metabolism; Stem Cells / cytology; Stem Cells / metabolism
TL;DR:
This platform provides a simple system to create a physiological oxygen gradient across an intestinal mimic while simultaneously supporting anaerobe co-culture.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: December 16, 2019
2019 journal article
Molecular transport through primary human small intestinal monolayers by culture on a collagen scaffold with a gradient of chemical cross-linking
JOURNAL OF BIOLOGICAL ENGINEERING, 13.
author keywords: Intestinal transport; Stiffness; Extracellular matrix; Small intestine
TL;DR:
The results of this comparison highlight the need to create in vitro intestinal transport platforms whose characteristics mimic the in vivo lamina propria in order to accurately recapitulate epithelial function.
(via Semantic Scholar)
open access via jbioleng.biomedcentral.com (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: May 13, 2019
2017 journal article
In Vitro Generation of Mouse Colon Crypts
ACS BIOMATERIALS SCIENCE & ENGINEERING, 3(10), 2502–2513.
author keywords: intestinal epithelial stem cells; differentiation; intestine-on-a-chip; microfabrication; gradient; tissue mimics
TL;DR:
A new approach combining microengineered scaffolds, native chemical gradients, and biophysical cues to control primary epithelium ex vivo can serve as a highly functional and physiologically relevant in vitro tissue model.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Automated microraft platform to identify and collect non-adherent cells successfully gene-edited with CRISPR-Cas9
BIOSENSORS & BIOELECTRONICS, 91, 175–182.
author keywords: Microraft; Cell array; CRISPR-Cas9; Cytometry; Cell sorting
MeSH headings : Biosensing Techniques / instrumentation; Biosensing Techniques / methods; CRISPR-Cas Systems; Equipment Design; Genes, Reporter; Green Fluorescent Proteins / genetics; Humans; Image Processing, Computer-Assisted; K562 Cells; Leukemia / genetics; Mutation; Splicing Factor U2AF / genetics; Tissue Array Analysis / instrumentation; Tissue Array Analysis / methods; Transfection
TL;DR:
The automated system was used to examine the temporal variation in EGFP expression in cells transfected with CRISPR-Cas9 components for gene editing and successfully gene edited two cell colonies demonstrating the desired mutation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Identification and isolation of antigen-specific cytotoxic T lymphocytes with an automated microraft sorting system
INTEGRATIVE BIOLOGY, 8(12), 1208–1220.
MeSH headings : Antigens, Viral / immunology; CD4-Positive T-Lymphocytes / immunology; CD8-Positive T-Lymphocytes / immunology; Cell Separation; Cells, Cultured; Epitope Mapping / instrumentation; Equipment Design; Equipment Failure Analysis; Humans; Immunoassay / instrumentation; Killer Cells, Natural / immunology; Lab-On-A-Chip Devices; T-Lymphocytes, Cytotoxic / immunology; Tissue Array Analysis / instrumentation
TL;DR:
A microraft array methodology is developed that automatically measures the ability of individual T cells to kill a population of target cells and viably sorts specific cells into a 96-well plate for expansion, enabling rapid identification of highly cytotoxic CD8+ T cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
In vitro polarization of colonoids to create an intestinal stem cell compartment
PLoS One, 11(4).
Source: NC State University Libraries
Added: August 6, 2018
2015 journal article
Array-Based Platform To Select, Release, and Capture Epstein-Barr Virus-Infected Cells Based on Intercellular Adhesion
ANALYTICAL CHEMISTRY, 87(24), 12281–12289.
MeSH headings : Cell Adhesion; Cell Separation / instrumentation; Cell Separation / methods; Cell Survival; Dimethylpolysiloxanes / chemistry; Epstein-Barr Virus Nuclear Antigens / genetics; Epstein-Barr Virus Nuclear Antigens / metabolism; Herpesvirus 4, Human / physiology; Humans; K562 Cells; Microarray Analysis / instrumentation; Nylons / chemistry; Particle Size; Single-Cell Analysis / instrumentation; Surface Properties; Tumor Cells, Cultured; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR:
Microraft arrays are a versatile method enabling separation of cells based on complicated and as yet poorly understood cell phenotypes, enabling release and collection of the cells without the need for cell attachment to the microraft surface.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Isolation and in Vitro Culture of Rare Cancer Stem Cells from Patient-Derived Xenografts of Pancreatic Ductal Adenocarcinoma
ANALYTICAL CHEMISTRY, 85(15), 7271–7278.
MeSH headings : Animals; Carcinoma, Pancreatic Ductal / pathology; Cell Culture Techniques / methods; Cell Line, Tumor; Cell Separation / methods; Cell Survival; Cell Transformation, Neoplastic; Humans; Image Processing, Computer-Assisted; Mice; Neoplastic Stem Cells / pathology; Pancreatic Neoplasms / pathology
TL;DR:
A direct comparison of this technology with fluorescence-activated cell sorting (FACS) demonstrated that micropallet arrays offered enhanced post sorting purity (100), yield (100%), and viability (94-100%) for rare cell isolation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Micropallet arrays for the capture, isolation and culture of circulating tumor cells from whole blood of mice engrafted with primary human pancreatic adenocarcinoma
BIOSENSORS & BIOELECTRONICS, 54, 476–483.
author keywords: Microfabrication; Surface functionalization; Cell array; Circulating tumor cells; Cell sorting; Pancreatic cancer
MeSH headings : Adenocarcinoma / blood; Adenocarcinoma / pathology; Animals; Biosensing Techniques / instrumentation; Cell Line, Tumor; Cell Separation / instrumentation; Equipment Design; Female; Humans; Mice; Neoplastic Cells, Circulating / pathology; Pancreatic Neoplasms / blood; Pancreatic Neoplasms / pathology; Tissue Array Analysis / instrumentation
TL;DR:
Clinical utility of this technology was shown through the capture, isolation and successful culture of CTCs from the blood of mice engrafted with primary human pancreatic tumors and direct capture and isolation of living tumor cells from blood followed by analysis or culture will be a valuable tool for cancer cell characterization.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
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