@article{duin_montalban_joglekar_d՚amico-willman_ritchie_fagen_huerta_2024, title={First Report of Bacterial Leaf Spot Disease on Sesame (<i>Sesamum indicum</i>) Caused by <i>Pseudomonas syringae</i> pv. <i>sesami</i> in North Carolina}, volume={108}, ISSN={0191-2917 1943-7692}, url={http://dx.doi.org/10.1094/PDIS-02-24-0292-PDN}, DOI={10.1094/PDIS-02-24-0292-PDN}, abstractNote={In July 2022, dark brown to black, angular, water-soaked lesions were observed on sesame leaves (Sesamum indicum L.) in a research plot established to assess yield potential for eight varieties at the North Carolina (NC) Sandhills Research Station (Chavez 2023). Symptoms were indicative of a bacterial leaf spot (BLS). At early flowering stage, leaf spots were present on scattered plants; varieties ES108, SS3301, and ES201 exhibited up to 75% disease prevalence, with lower frequency in ES103, S39, S4302, S3251, and S3276. Symptomatic leaves from 3-4 plants were collected on four different dates from July through September. A section of symptomatic tissue was excised and macerated in sterile deionized water (SDW). A 10 µL aliquot was streaked onto SPA medium (15 g sucrose, 5.0 g proteose peptone, 0.50 g MgSO4 7H2O, 0.25 g K2HPO4, 15 g agar per liter of SDW) and incubated at 28ºC. After 72 h, numerous, smooth, white-cream colored, convex-shaped, colonies were individually isolated. Five randomly selected isolates from the different collection dates, designated as AHP108-AHP111 and AHP116, were genotyped. The 16S rRNA, gyrB, rpoD, and gapA genes were sequenced (Heuer et al. 1997; Hwang et al. 2005) and deposited to NCBI (GenBank Accessions: P213467- PP213470; OQ628040-OQ628042; PP214983-PP214994; and PP255798). These five isolates shared 100% sequence identity for gyrB and rpoD. AHP108-AHP111 shared 100% sequence identity for 16S rRNA and gapA, with 99.7% and 90.8% identity, respectively, for AHP116. A phylogenetic tree was inferred from a maximum-likelihood analysis of concatenated gyrB, rpoD, and gapA sequences of the five isolates and the top 11 hts from a blastn search of the NCBI nucleotide database. Those hits included closely related sequences from Pseudomonas syringae pv. sesami type strains ICMP 763T and ICMP 7459T. Based on this phylogenetic analysis AHP108-AHP111 and AHP116 are P. syringae pv. sesami. Recent genomic analysis suggests this pathovar is part of P. amygdali (Gomila et al. 2017), but an official name change has not been proposed. Each of the five isolates were infiltrated into leaves of sesame varieties ES108, ES103, and S327, consistently resulting in similar symptoms. Thus, strain AHP116, as a representative, was used to fulfill Koch's postulates using five, 30-day-old potted sesame plants (var. S3301). Plants were spray-inoculated with a bacterial suspension of ~108 CFU/ml until runoff; plants were incubated in moist chambers 24 h pre and post inoculation at 28ºC with 80% relative humidity and a 12 h photoperiod. At 13 days post inoculation, symptoms resembling those on plants at the Sandhills Research Stations in 2022 were evident. Reisolated bacteria were confirmed to be AHP116 through 16S rRNA and gyrB amplification and sequencing. No symptoms were observed on the five water-inoculated plants. BLS of sesame has been reported in Asia and is thought to be seedborne (Firdous et al. 2009; Prathuangwong and Yowabutra 1997). To our knowledge, this is the first report of P. syringae pv. sesami causing BLS on sesame in North Carolina. Sesame cultivation in the state increased from approximately 2,000 acres in 2022 to 13,000 acres in 2023 and there is interest in cultivating sesame as a rotational and alternative crop because it requires minimal input costs. Potential outbreaks of BLS in this warm, humid region could negatively affect sesame production, where little is known about the economic impact of the disease.}, number={6}, journal={Plant Disease}, publisher={Scientific Societies}, author={Duin, Izabela Moura and Montalban, Kimberly M. and Joglekar, Prasanna and D՚Amico-Willman, Katherine and Ritchie, David F. and Fagen, Jennie R. and Huerta, Alejandra I.}, year={2024}, month={Jun}, pages={1880} }
 @article{d'amico-willman_montalban_joglekar_duin_ritchie_fagen_huerta_2024, title={Whole-Genome Sequence Resources for <i>Pseudomonas amygdali</i> pv. <i>sesami</i> and <i>Xanthomonas arboricola</i> Isolated from Sesame (<i>Sesamum indicum</i>) in North Carolina in 2022}, volume={7}, ISSN={2690-5442}, url={http://dx.doi.org/10.1094/PHYTOFR-11-23-0148-A}, DOI={10.1094/PHYTOFR-11-23-0148-A}, abstractNote={Sesame ( Sesamum indicum) is a specialty crop with increasing interest as an alternative, rotational crop for U.S. agricultural systems. This crop is susceptible to several pathogens, including Pseudomonas and Xanthomonas species. Two bacterial isolates were recently cultured from sesame in North Carolina symptomatic of bacterial spot and identified as Pseudomonas amygdali pv. sesami (Pas) and Xanthomonas sp. These isolates were used for hybrid whole-genome sequencing and assembly using Illumina and PacBio to develop robust genomic resources for these disease-causing strains. The results provide the most complete Pas genome available and placed the Xanthomonas sp. isolate into the X. arboricola species designation. Improved genomic resources for pathogens of sesame are needed to accurately detect, characterize, and employ timely management of the disease. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .}, journal={PhytoFrontiers™}, publisher={Scientific Societies}, author={D'Amico-Willman, Katherine M. and Montalban, Kimberly M. and Joglekar, Prasanna and Duin, Izabela Moura and Ritchie, David F. and Fagen, Jennie and Huerta, Alejandra I.}, year={2024}, month={Jul} }
 @article{d’amico-willman_joglekar_luna_ritchie_fagen_huerta_2022, title={Complete Genome Sequence of Xanthomonas arboricola pv.
            <i>pruni
            Strain Xcp1 Isolated in 1984 from a Bacterial Spot Spring Canker on Prunus persica var.
            <i>nucipersica
            cv. “Redgold”}, volume={11}, ISSN={2576-098X}, url={http://dx.doi.org/10.1128/mra.00209-22}, DOI={10.1128/mra.00209-22}, abstractNote={
            Xanthomonas arboricola
            pv.
            pruni
            is an important plant pathogen and the causal agent of bacterial spot of stone fruits (
            Prunus
            spp). Here, we report a complete genome of
            X. arboricola
            pv.
            pruni
            strain Xcp1 generated from hybrid PacBio Sequel and Illumina NextSeq2000 sequencing.
          }, number={12}, journal={Microbiology Resource Announcements}, publisher={American Society for Microbiology}, author={D’Amico-Willman, Katherine M. and Joglekar, Prasanna and Luna, Emily K. and Ritchie, David F. and Fagen, Jennie and Huerta, Alejandra I.}, editor={Dunning Hotopp, Julie C.Editor}, year={2022}, month={Dec} }