A flow cytometric method for estimating S-phase duration in plants
Mickelson-Young, L., Wear, E., Mulvaney, P., Lee, T.-J., Szymanski, E. S., Allen, G., … Thompson, W. (2016, October 3). Journal of Experimental Botany, Vol. 11, pp. 6077–6087.
author keywords: Arabidopsis; barley; EdU; flow cytometry; maize; replication timing; rice; S-phase duration; wheat
MeSH headings : Arabidopsis / cytology; Arabidopsis / growth & development; DNA, Plant / metabolism; Deoxyuridine / analogs & derivatives; Deoxyuridine / metabolism; Flow Cytometry / methods; G1 Phase / physiology; G2 Phase / physiology; Hordeum / cytology; Hordeum / growth & development; Meristem / cytology; Meristem / growth & development; Oryza / cytology; Oryza / growth & development; S Phase / physiology; Triticum / cytology; Triticum / growth & development; Zea mays / cytology; Zea mays / growth & development
topics (OpenAlex): Chromosomal and Genetic Variations; Plant nutrient uptake and metabolism; Plant Genetic and Mutation Studies
TL;DR:
This work estimated S-phase duration for several plant species by following EdU-labeled nuclei from G1 to G2 using bivariate flow cytometry, finding that S- phase duration is relatively consistent over a range of genome sizes.
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