Patrick K. Mulvaney

Works (2)

Updated: July 5th, 2023 15:37

2017 journal article

Genomic Analysis of the DNA Replication Timing Program during Mitotic S Phase in Maize (Zea mays) Root Tips

PLANT CELL, 29(9), 2126–2149.

By: E. Wear n, J. Song *, G. Zynda *, C. LeBlanc *, T. Lee n, L. Mickelson-Young n, L. Concia  n, P. Mulvaney n ...

MeSH headings : Base Sequence; Chromosomes, Plant / genetics; DNA Replication Timing / genetics; DNA Transposable Elements / genetics; Genes, Plant; Genomics; Meristem / cytology; Meristem / genetics; Mitosis / genetics; Models, Genetic; S Phase / genetics; Tandem Repeat Sequences / genetics; Time Factors; Transcription, Genetic; Zea mays / cytology; Zea mays / genetics
TL;DR: A novel adaptation of the “Repli-seq” assay for use in intact root tips of maize (Zea mays) that includes several different cell lineages and present whole-genome replication timing profiles from cells in early, mid, and late S phase of the mitotic cell cycle is reported. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2016 journal article

A flow cytometric method for estimating S-phase duration in plants

JOURNAL OF EXPERIMENTAL BOTANY, 67(21), 6077–6087.

By: L. Mickelson-Young n, E. Wear n, P. Mulvaney n, T. Lee n, E. Szymanski  n, G. Allen n, L. Hanley-Bowdoin n , W. Thompson n 

author keywords: Arabidopsis; barley; EdU; flow cytometry; maize; replication timing; rice; S-phase duration; wheat
MeSH headings : Arabidopsis / cytology; Arabidopsis / growth & development; DNA, Plant / metabolism; Deoxyuridine / analogs & derivatives; Deoxyuridine / metabolism; Flow Cytometry / methods; G1 Phase / physiology; G2 Phase / physiology; Hordeum / cytology; Hordeum / growth & development; Meristem / cytology; Meristem / growth & development; Oryza / cytology; Oryza / growth & development; S Phase / physiology; Triticum / cytology; Triticum / growth & development; Zea mays / cytology; Zea mays / growth & development
TL;DR: This work estimated S-phase duration for several plant species by following EdU-labeled nuclei from G1 to G2 using bivariate flow cytometry, finding that S- phase duration is relatively consistent over a range of genome sizes. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

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