Works (27)
Updated: April 22nd, 2024 04:49
2024 journal article
Characterization of an Injectable Chitosan Hydrogel for the Tunable, Localized Delivery of Immunotherapeutics
ACS BIOMATERIALS SCIENCE & ENGINEERING, 10(2), 905–920.
author keywords: hydrogel; chitosan; immunotherapy; intratumoral
TL;DR:
The goal of the present work was to construct a novel, injectable hydrogel that could be tuned for localized immunotherapy delivery and displayed several clinically important features, including injectability, biocompatibility, and imageability.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: March 4, 2024
2024 article
Comparison of the Backbone Dynamics of Dehaloperoxidase-Hemoglobin Isoenzymes
Gonzalez-Delgado, J. M., Thompson, P. M., Andralojc, W., Gdaniec, Z., Ghiladi, R. A., & Franzen, S. (2024, April 2). JOURNAL OF PHYSICAL CHEMISTRY B, Vol. 4.
Sources: Web Of Science, NC State University Libraries
Added: April 15, 2024
2024 journal article
Fructose regulates the pentose phosphate pathway and induces an inflammatory and resolution phenotype in Kupffer cells
SCIENTIFIC REPORTS, 14(1).
TL;DR:
It is demonstrated that chronic fructose diet decreased Kupffer cell populations while increasing transitioning monocytes and upregulating resolution and anti-inflammatory associated genes in Kupffer cells.
(via Semantic Scholar)
doi.org (publisher)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: March 25, 2024
2023 journal article
Excision of a Protein-Derived Amine for <i>p</i>-Aminobenzoate Assembly by the Self-Sacrificial Heterobimetallic Protein CADD
BIOCHEMISTRY, 62(22), 3276–3282.
TL;DR:
Turnover studies using limiting O2 have identified a para-aminobenzaldehyde metabolic intermediate that is formed on the path to pABA formation and use of pABCHO and other probe substrates shows that the heterobimetallic Fe/Mn form of the enzyme is capable of oxygen insertion to generate the pA BA-carboxylate.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: December 12, 2023
2022 journal article
An adaptive teosinte mexicana introgression modulates phosphatidylcholine levels and is associated with maize flowering time
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 119(27).
author keywords: phospholipid metabolism; maize genetics; highland adaptation; flowering time; selection
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
15. Life on Land
(Web of Science; OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: August 3, 2022
2021 journal article
The mosquito protein AEG12 displays both cytolytic and antiviral properties via a common lipid transfer mechanism.
Proceedings of the National Academy of Sciences of the United States of America, 118(11).
author keywords: antiviral; membrane disruption; cytolysis; insect digestion; insect immune response
MeSH headings : Animals; Antiviral Agents / chemistry; Antiviral Agents / metabolism; Antiviral Agents / pharmacology; Cell Line; Cell Membrane / metabolism; Culicidae; Erythrocytes / drug effects; Fatty Acids, Unsaturated / metabolism; Hemolytic Agents / chemistry; Hemolytic Agents / metabolism; Hemolytic Agents / pharmacology; Humans; Hydrophobic and Hydrophilic Interactions; Insect Proteins / chemistry; Insect Proteins / metabolism; Insect Proteins / pharmacology; Ligands; Lipids / chemistry; Protein Binding; Protein Structure, Tertiary; Viral Envelope / metabolism; Viruses / drug effects; Viruses / metabolism
TL;DR:
The three-dimensional structure of AEG12 is determined and the binding specificity of acyl-chain ligands within its large central hydrophobic cavity is described, enabling it to inhibit replication of enveloped viruses such as Dengue and Zika viruses at low micromolar concentrations.
(via Semantic Scholar)
Source: ORCID
Added: March 14, 2021
2020 journal article
Mixture analyses of air-sampled pollen extracts can accurately differentiate pollen taxa
ATMOSPHERIC ENVIRONMENT, 243.
author keywords: Pollen; NMR; Metabolomics; Mixtures; Exposure; Aerobiology
TL;DR:
NMR spectra of air-sampled pollen extracts can be used in an automated fashion to provide taxa and type-specific measures of the daily pollen count, and deep learning models using convolutional neural networks performed better than regression models using NMR spectral input.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 24, 2020
2019 journal article
Hydrophobic ligands influence the structure, stability, and processing of the major cockroach allergen Bla g 1
Scientific Reports, 9(1).
MeSH headings : Allergens / chemistry; Allergens / immunology; Animals; Antigen Presentation; Blattellidae / immunology; Fatty Acids / immunology; Fatty Acids / metabolism; Insect Proteins / chemistry; Insect Proteins / immunology; Ligands; Phospholipids / immunology; Phospholipids / metabolism; Protein Binding / immunology; Protein Stability; Protein Structure, Tertiary
TL;DR:
Atomic modelling of cockroach allergen Bla g 1 binds to both fatty-acid and phospholipids ligands, with 8 acyl chains suggested to represent full stoichiometric binding, with potential downstream implications for immunogenicity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: December 10, 2019
2019 conference paper
Influence of Hydrophobic Cargo Binding on the Structure, Stability, and Allergenicity of the Cockroach Allergen Bla g 1
Journal of Allergy and Clinical Immunology.
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: December 10, 2019
2019 journal article
Multiple roles of Bet v 1 ligands in allergen stabilization and modulation of endosomal protease activity.
Allergy.
author keywords: allergenicity; birch pollen extract; E-1 phytoprostanes; ligand interaction; lysosomal protease inhibition
MeSH headings : Allergens / immunology; Antigens, Plant / immunology; Basophils / immunology; Basophils / metabolism; Betula / immunology; Cell Degranulation / immunology; Endosomes / enzymology; Enzyme Activation; Humans; Immunoglobulin E / immunology; Ligands; Peptide Hydrolases / metabolism; Pollen / immunology; Protein Binding; Recombinant Proteins
TL;DR:
How known birch pollen–derived molecules influence the endolysosomal processing of Bet v 1, thereby shaping its allergenicity is examined.
(via Semantic Scholar)
Source: ORCID
Added: May 12, 2021
2017 journal article
A Structural Model for Vinculin Insertion into PIP<sub>2</sub>-Containing Membranes and the Effect of Insertion on Vinculin Activation and Localization.
Structure (London, England : 1993).
MeSH headings : Actin Cytoskeleton / genetics; Actin Cytoskeleton / metabolism; Actins / genetics; Actins / metabolism; Amino Acid Motifs; Animals; Binding Sites; Embryo, Mammalian; Fibroblasts / metabolism; Fibroblasts / ultrastructure; Focal Adhesions / metabolism; Focal Adhesions / ultrastructure; Gene Expression; Hydrophobic and Hydrophilic Interactions; Lipid Bilayers / chemistry; Lipid Bilayers / metabolism; Mechanotransduction, Cellular; Mice; Molecular Docking Simulation; Molecular Dynamics Simulation; Mutation; Nuclear Magnetic Resonance, Biomolecular; Phosphatidylinositol 4,5-Diphosphate / chemistry; Phosphatidylinositol 4,5-Diphosphate / metabolism; Protein Binding; Protein Conformation, alpha-Helical; Protein Interaction Domains and Motifs; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Thermodynamics; Vinculin / chemistry; Vinculin / genetics; Vinculin / metabolism
TL;DR:
Results indicate that PIP2 binding is not required for localization of vinculin to FAs or FA strengthening, but is required for vincul activation and turnover at FAs to promote its association with the force transduction FA nanodomain.
(via Semantic Scholar)
Source: ORCID
Added: May 12, 2021
2017 conference paper
Role of PIP2-Dependent Membrane Interactions in Vinculin Activation, Motility and Force Transmission
Biophysical Journal.
TL;DR:
This work proposes an alternative model of vinculin/PIP2 interaction that is compatible with membrane insertion, using experimental data, molecular docking and dynamics simulations, and provides validation of the model through biophysical and biochemical analyses.
(via Semantic Scholar)
Source: ORCID
Added: December 10, 2019
2016 journal article
A metabolomic, geographic, and seasonal analysis of the contribution of pollen-derived adenosine to allergic sensitization
Metabolomics.
author keywords: NMR; Metabolomics; Pollen; Adenosine; Allergy
TL;DR:
It would be a rare event limited to tree pollen season for concentrations of pollen-derived adenosine to approach physiological levels, and sugar content and other metabolites may be useful in classifying pollens.
(via Semantic Scholar)
Source: ORCID
Added: December 10, 2019
2016 journal article
Role of PIP2-Dependent Membrane Interactions in Vinculin Activation, Motility and Force Transmission
Biophysical Journal, 110(3), 575a.
Source: ORCID
Added: December 10, 2019
2016 journal article
The Structural Basis of Actin Organization by Vinculin and Metavinculin
Journal of Molecular Biology, 428(1), 10–25.
author keywords: cryo-EM; cytoskeleton; mechanobiology; adhesion; cell motility
MeSH headings : Actins / chemistry; Actins / metabolism; Cryoelectron Microscopy; Humans; Models, Molecular; Protein Binding; Protein Conformation; Protein Multimerization; Vinculin / chemistry; Vinculin / metabolism
TL;DR:
An 8.5-Å-resolution cryo-electron microscopy reconstruction and pseudo-atomic model of the vinculin tail (Vt) domain bound to F-actin is presented, finding that an analogous conformational change also occurs in the H1' helix of the tail domain of metavinculin (MVT) upon actin binding, a muscle-specific splice isoform that suppresses actin bundling by Vt.
(via Semantic Scholar)
Source: ORCID
Added: December 10, 2019
2015 article
MDFF model of the vinculin tail domain bound to F-actin
Kim, L. Y., Thompson, P. M., Lee, H. T., Pershad, M., Campbell, S. L., & Alushin, G. M. (2015, November 4).
Source: ORCID
Added: December 10, 2019
2015 journal article
New Models for Regulation of Vinculin by Actin and Phospholipids
Biophysical Journal, 108(2), 508a–509a.
TL;DR:
A sub-nanometer resolution reconstruction of the Vt/actin complex is obtained which sheds light on actin-induced conformational changes necessary for vinculin dimerization and actin filament bundling.
(via Semantic Scholar)
Source: ORCID
Added: December 10, 2019
2015 article
Protein-Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy
Protein-Protein Interactions, pp. 267–279.
author keywords: Pulsed-field gel electrophoresis (PFGE); Salmonella enterica; Molecular typing; PulseNet; Agarose gel; Enteric pathogens
MeSH headings : Magnetic Resonance Spectroscopy / methods; Molecular Docking Simulation; Protein Binding; Protein Interaction Mapping / methods; Proteins / chemistry; Thermodynamics
TL;DR:
A straightforward method to map a protein-protein interface and design a structural model is described, using chemical shift perturbation, paramagnetic relaxation enhancement, and data-driven docking.
(via Semantic Scholar)
Source: ORCID
Added: December 10, 2019
2014 journal article
Correction to Phosphorylation at Y1065 in Vinculin Mediates Actin Bundling, Cell Spreading, and Mechanical Responses to Force
Biochemistry, 53(39), 6286–6286.
TL;DR:
This work was supported by National Institutes of Health Grants K.L.C.) and NIH/NIBIB 5P41EB002025 (R.S.).
(via Semantic Scholar)
Source: ORCID
Added: December 10, 2019
2014 journal article
Identification of an Actin Binding Surface on Vinculin that Mediates Mechanical Cell and Focal Adhesion Properties
Structure, 22(5), 697–706.
MeSH headings : Actins / metabolism; Animals; Avian Proteins / chemistry; Avian Proteins / genetics; Avian Proteins / metabolism; Binding Sites; Cells, Cultured; Fibroblasts / cytology; Focal Adhesions; Hydrophobic and Hydrophilic Interactions; Mice; Microscopy, Electron / methods; Models, Molecular; Point Mutation; Rabbits; Vinculin / chemistry; Vinculin / genetics; Vinculin / metabolism
TL;DR:
The identification of actin-binding deficient mutants of vinculin outside sites predicted to bind actin and an alternative model developed provide the molecular basis for elucidating additional roles of this interaction, including act in-induced conformational changes that promote actin bundling.
(via Semantic Scholar)
Source: ORCID
Added: December 10, 2019
2014 journal article
Phosphorylation at Y1065 in Vinculin Mediates Actin Bundling, Cell Spreading, and Mechanical Responses to Force
Biochemistry, 53(34), 5526–5536.
MeSH headings : Actins / chemistry; Animals; Cells, Cultured; Circular Dichroism; Magnetic Resonance Spectroscopy; Mice; Microscopy, Fluorescence; Mutation; Phosphorylation; Protein Binding; Vinculin / chemistry; Vinculin / genetics; Vinculin / physiology
TL;DR:
It is shown that Src phosphorylation of Y1065 within the C-terminal hairpin regulates Vt-mediated actin bundling and provides a detailed characterization of Y 1065 mutations, and thatosphorylation at Y1055 plays a role in cell spreading and the response to the application of mechanical force.
(via Semantic Scholar)
Source: ORCID
Added: December 10, 2019
2014 journal article
SketchBio: a scientist’s 3D interface for molecular modeling and animation
BMC Bioinformatics, 15(1).
author keywords: Molecular modelling; Animation; Collision detection
MeSH headings : Computer Simulation; Humans; Models, Molecular; Molecular Conformation; Software
TL;DR:
SketchBio is presented, a tool that incorporates state-of-the-art bimanual interaction and drop shadows to enable rapid construction of molecular structures and animations and includes three novel features: crystal-by-example, pose-mode physics, and spring-based layout that accelerate operations common in the formation of molecular models.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 4, 2020
2013 journal article
Combined computational design of a zinc-binding site and a protein-protein interaction: One open zinc coordination site was not a robust hotspot for de novo ubiquitin binding
Proteins, 81(9), 1678–1679.
Source: ORCID
Added: December 10, 2019
2013 journal article
Combined computational design of a zinc-binding site and a protein-protein interaction: One open zinc coordination site was not a robust hotspot for de novo ubiquitin binding
Proteins: Structure, Function, and Bioinformatics, 81(7), 1245–1255.
author keywords: computational interface design; de novo; heterodimer; metal coordination; zinc binding; protein-protein interaction
MeSH headings : Alanine / chemistry; Binding Sites; Magnetic Resonance Spectroscopy; Mutagenesis; Protein Binding; Protein Interaction Maps; Ubiquitin / chemistry; Zinc / chemistry
TL;DR:
This study led us to conclude that 2/2 coordination arrangements or multiple‐zinc designs are more likely to promote high‐affinity protein interactions.
(via Semantic Scholar)
Sources: ORCID, Crossref, NC State University Libraries
Added: December 10, 2019
2013 journal article
Vinculin and metavinculin: Oligomerization and interactions with F-actin
FEBS Letters, 587(8), 1220–1229.
author keywords: Vinculin; Metavinculin; Actin; PIP2; Oligomerization
MeSH headings : Actin Cytoskeleton / chemistry; Actin Cytoskeleton / metabolism; Actins / chemistry; Actins / metabolism; Humans; Models, Molecular; Protein Binding; Protein Multimerization; Protein Structure, Tertiary; Vinculin / chemistry; Vinculin / genetics; Vinculin / metabolism
TL;DR:
Differences in these functions between vinculin and metavinculin provide key insights to the structure and function of these oligomers, and should guide further studies.
(via Semantic Scholar)
Source: ORCID
Added: December 10, 2019
2013 journal article
Vinculin–actin interaction couples actin retrograde flow to focal adhesions, but is dispensable for focal adhesion growth
J Cell Biol, 202(1), 163–177.
MeSH headings : Actins / metabolism; Amino Acid Substitution; Animals; Cell Movement; Cloning, Molecular; Extracellular Matrix / metabolism; Fibroblasts / metabolism; Focal Adhesions / genetics; Focal Adhesions / metabolism; Green Fluorescent Proteins / metabolism; Mice; Mice, Inbred C57BL; Point Mutation; Protein Binding; Protein Interaction Mapping / methods; Protein Transport; Proteolysis; Pseudopodia / metabolism; Vinculin / genetics; Vinculin / metabolism
TL;DR:
Vinculin functions as a molecular clutch that organizes leading edge F-actin, generates traction, and promotes focal adhesion formation and turnover but not adhesion growth.
(via Semantic Scholar)
Source: ORCID
Added: December 10, 2019
2008 journal article
Phthalate induction of CYP3A4 is dependent on glucocorticoid regulation of PXR expression
TOXICOLOGICAL SCIENCES, 103(2), 268–277.
author keywords: pregnane X receptor; CYP3A4; DEHP; MEHP; glucocorticoid
MeSH headings : Animals; Carcinoma, Hepatocellular; Cell Line, Tumor; Cytochrome P-450 CYP3A / biosynthesis; Dexamethasone / pharmacology; Diethylhexyl Phthalate / analogs & derivatives; Diethylhexyl Phthalate / toxicity; Dose-Response Relationship, Drug; Drug Synergism; Enzyme Induction / drug effects; Glucocorticoids / pharmacology; Hepatocytes / drug effects; Hepatocytes / metabolism; Humans; Plasticizers / toxicity; Pregnane X Receptor; Rats; Receptors, Steroid / drug effects; Receptors, Steroid / metabolism; Transcription, Genetic / drug effects; Transfection
TL;DR:
It is suggested that the magnitude of CYP3A4 induction by phthalates is dependent on the expression of PXR and may be significantly higher in the presence of glucocorticoids and may play a role in altered efficacy of pharmaceutical agents.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: ORCID, Web Of Science
Added: August 6, 2018
Employment
Updated: June 21st, 2023 11:48
2019 - present
North Carolina State University Raleigh, North Carolina, US
Adjunct Research Assistant Professor
Molecular and Structural Biochemistry
2018 - present
North Carolina State University Raleigh, NC, US
Lead Research Scientist
Molecular Education, Technology, Research and Innovation Center (METRIC)
2017 - 2019
North Carolina State University Raleigh, North Carolina, US
Principal Research Scholar
Biochemistry
2015 - 2017
National Institute of Environmental Health Sciences NC, NC, US
Postdoctoral Fellow
Genome Integrity and Structural Biology
Education
Updated: April 30th, 2021 12:16
2008 - 2015
University of North Carolina at Chapel Hill Chapel Hill, NC, US
PhD Candidate
Biochemistry and Biophysics
2004 - 2008
North Carolina State University Raleigh, NC, US
B.S.
Biochemistry
Funding History
Funding history based on the linked ORCID record. Updated: December 10th, 2019 14:26
grant
July 1, 2012 - June 30, 2014
Vinculin Tail Allostery in F-actin Interactions and Implications for Response to Mechanical Force
American Heart Association
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