Patrick V Gurgel

Works (29)

Updated: August 23rd, 2023 21:14

2019 journal article

Use of a Branched Linker for Enhanced Biosensing Properties in IgG Detection from Mixed Chinese Hamster Ovary Cell Cultures

BIOCONJUGATE CHEMISTRY, 30(3), 815–825.

By: N. Islam n, P. Gurgel n, O. Rojas n & R. Carbonell n

MeSH headings : Adsorption; Amino Acid Sequence; Animals; Biosensing Techniques; CHO Cells; Complex Mixtures; Cricetinae; Cricetulus; Immunoglobulin G / analysis; Kinetics; Peptides / chemistry
TL;DR: The branched amines increased the overall sensitivity and selectivity for IgG adsorption from complex mixtures of Chinese hamster ovary supernatant (CHO) and regeneration studies were carried out. (via Semantic Scholar)
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6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: April 9, 2019

2016 journal article

Reducing diffusion limitations in Ion exchange grafted membranes using high surface area nonwovens

JOURNAL OF MEMBRANE SCIENCE, 514, 53–64.

By: M. Heller n, R. Wimbish n, P. Gurgel n, B. Pourdeyhimi n  & R. Carbonell n

author keywords: UV grafting; Islands-in-the-sea nonwoven; Diffusion limited; Ion exchange protein capture; Shrinking core
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UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Affinity interactions of human immunoglobulin G with short peptides: role of ligand spacer on binding, kinetics, and mass transfer

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 408(7), 1829–1841.

By: F. Shen  n, O. Rojas n, J. Genzer n , P. Gurgel n & R. Carbonell n

author keywords: Peptide ligands; Immunoglobulin G; Peptide HWRGWV; Spacer arms; Quartz crystal microbalance
MeSH headings : Adsorption; Amino Acid Sequence; Humans; Immunoglobulin G / metabolism; Kinetics; Ligands; Peptides / chemistry; Peptides / metabolism; Polyethylene Glycols / chemistry; Protein Binding; Quartz Crystal Microbalance Techniques / methods; Silicon Dioxide / chemistry
TL;DR: Light was shed on interfacial effects important for detection and bioseparation by following the shifts in frequency and energy dissipation in a quartz crystal microbalance, as well as thermodynamic and kinetics parameters. (via Semantic Scholar)
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7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Preparation and characterization of anion exchange adsorptive nonwoven membranes with high protein binding capacity

JOURNAL OF MEMBRANE SCIENCE, 493, 349–359.

By: H. Liu n, P. Gurgel n & R. Carbonell n

author keywords: Anion exchange membrane; Protein purification; Nonwoven surface modification; High protein binding capacity; hIgG separation
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UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Dynamic and equilibrium performance of sensors based on short peptide ligands for affinity adsorption of human IgG using surface plasmon resonance

BIOSENSORS & BIOELECTRONICS, 58, 380–387.

By: N. Islam n, F. Shen  n, P. Gurgel n, O. Rojas n & R. Carbonell n

author keywords: Hexamer; Protein A; Human immunoglobulin G; Self-assembled monolayers; Adsorption rate constant; Desorption rate constant
MeSH headings : Adsorption; Antibody Affinity / immunology; Binding Sites; Biosensing Techniques / instrumentation; Equipment Design; Equipment Failure Analysis; Immunoglobulin G / chemistry; Immunoglobulin G / immunology; Peptides / chemistry; Peptides / immunology; Protein Binding; Protein Interaction Mapping / instrumentation; Surface Plasmon Resonance / instrumentation
TL;DR: This paper characterizes the potential of novel hexameric peptide ligands for on-line IgG detection in bioprocesses and finds that the peptide-IgG binding was reaction controlled, however the protein A-IGG binding mechanism was partially mass transfer (diffusion) controlled. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2014 journal article

Effects of Composition of Oligo(ethylene glycol)-Based Mixed Monolayers on Peptide Grafting and Human Immunoglobulin Detection

JOURNAL OF PHYSICAL CHEMISTRY C, 118(10), 5361–5373.

By: N. Islam n, P. Gurgel n, O. Rojas n & R. Carbonell n

TL;DR: The specificity toward IgG was optimal for peptides grafted on SAMs prepared from 10% EG6NH2 precursor solution, even though this surface did not have the highest number of peptides per unit area. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Affinity chromatographic purification of human immunoglobulin M from human B lymphocyte cell culture supernatant

BIOCHEMICAL ENGINEERING JOURNAL, 70, 63–70.

By: Z. Liu n, P. Gurgel n & R. Carbonell n

author keywords: Affinity chromatography; Hexamer peptide; Human immunoglobulin; IgM; Dynamic binding capacity; Elution additive
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Source: Web Of Science
Added: August 6, 2018

2012 journal article

Affinity chromatographic purification of human immunoglobulin a from chinese hamster ovary cell culture supernatant

BIOTECHNOLOGY PROGRESS, 29(1), 91–98.

By: Z. Liu n, P. Gurgel n & R. Carbonell n

author keywords: affinity chromatography; hexamer peptide; CHO; human immunoglobulin; secretory IgA
MeSH headings : Adsorption; Animals; CHO Cells; Cells, Cultured; Chromatography, Affinity; Cricetinae; Cricetulus; Humans; Immunoglobulin A / isolation & purification; Oligopeptides / chemical synthesis; Oligopeptides / chemistry; Surface Properties
TL;DR: The peptide ligand resins exhibit great potential for large‐scale purification of both human IgA and secretory IgA, respectively, and the DBC value of HWRGWV for IgA is much greater than current commercially available affinity ligands. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2012 journal article

Alkaline-stable peptide ligand affinity adsorbents for the purification of biomolecules

Journal of Chromatography A, 1245, 55–64.

By: S. Menegatti n , A. Naik n, P. Gurgel n & R. Carbonell n

author keywords: Peptide ligand; Affinity adsorbent; NaOH stability; Modification of surface chemistry; Solid-phase peptide synthesis; Cleaning-in-place
MeSH headings : Adsorption; Alkalies / chemistry; Chromatography, Affinity / instrumentation; Chromatography, Affinity / methods; Immunoglobulin G / chemistry; Immunoglobulin G / isolation & purification; Ligands; Peptides / chemistry; Protein Binding
TL;DR: It was found that the ligand leaching was caused by the coupling of a significant amount of peptide by alkaline-labile ester bonds, and the method presented has a broad application since it is valid for any peptide ligand identified for the purification of a biopharmaceutical target. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Process for purification of monoclonal antibody expressed in transgenic Lemna plant extract using dextran-coated charcoal and hexamer peptide affinity resin

Journal of Chromatography A, 1260, 61–66.

By: A. Naik n, S. Menegatti n , H. Reese n, P. Gurgel n & R. Carbonell n

author keywords: Dextran-coated charcoal; Transgenic plants; Hexapeptide ligand; IgG purification; Monoclonal antibody; Phenolics; Lemna minor
MeSH headings : Antibodies, Monoclonal / isolation & purification; Antibodies, Monoclonal / metabolism; Araceae / chemistry; Araceae / metabolism; Charcoal / chemistry; Chromatography, Affinity / methods; Dextrans / chemistry; Humans; Immunoglobulin G / isolation & purification; Immunoglobulin G / metabolism; Peptides / chemistry; Peptides / metabolism; Plant Extracts / chemistry; Plants, Genetically Modified / chemistry; Plants, Genetically Modified / metabolism; Protein Binding; Staphylococcal Protein A / chemistry; Staphylococcal Protein A / isolation & purification
TL;DR: The development of a pretreatment and affinity separation process for the purification of a monoclonal antibody from transgenic Lemna plant extract and the performance of the hexamer peptide resin after the pretreatment step was found to be similar to that obtained with a commercial Protein A resin. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Purification of human immunoglobulins A, G and M from Cohn fraction II/III by small peptide affinity chromatography

JOURNAL OF CHROMATOGRAPHY A, 1262, 169–179.

By: Z. Liu n, P. Gurgel n & R. Carbonell n

author keywords: Hexamer peptide; IVIG; Cohn fraction; Elution additive; PEG; Ammonium sulfate
MeSH headings : Ammonium Sulfate / chemistry; Arginine / chemistry; Blood Proteins / chemistry; Caprylates / chemistry; Chromatography, Affinity / methods; Ethylene Glycols / chemistry; Humans; Immunoglobulin Isotypes / blood; Immunoglobulin Isotypes / isolation & purification; Peptides / chemistry; Salts / chemistry
TL;DR: Attempts to purify human IgG, IgA and IgM from Cohn fraction II/III using HWRGWV affinity peptide resin using pretreatment methods comparable to current chromatographic purification methods involving two chromatography steps when hIgG is isolated from plasma fractions are described. (via Semantic Scholar)
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6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Effects of UV Exposure and Initiator Concentration on the Spatial Variation of Poly(glycidyl methacrylate) Grafts on Nonwoven Fabrics

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 50(10), 6115–6123.

By: Y. Zheng, P. Gurgel* & R. Carbonell n

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Source: Web Of Science
Added: August 6, 2018

2011 journal article

Effects of peptide density and elution pH on affinity chromatographic purification of human immunoglobulins A and M

JOURNAL OF CHROMATOGRAPHY A, 1218(46), 8344–8352.

By: Z. Liu n, P. Gurgel n & R. Carbonell n

author keywords: Human immunoglobulin; IgG; IgA; IgM; Affinity chromatography; Binding site
MeSH headings : Amino Acid Sequence; Binding Sites; Chromatography, Affinity / instrumentation; Chromatography, Affinity / methods; Electrophoresis, Polyacrylamide Gel; Humans; Hydrogen-Ion Concentration; Immunoglobulin A / chemistry; Immunoglobulin A / isolation & purification; Immunoglobulin M / chemistry; Immunoglobulin M / isolation & purification; Immunosorbent Techniques; Molecular Sequence Data; Oligopeptides / chemistry; Protein Conformation; Sequence Alignment
TL;DR: Although HYFKFD and HFRRHL exhibited their ability for isolation of human IgG, IgA and IgM from cMEM as well, HWRGWV is the best option among them for large-scale purification (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2011 journal article

Porcine parvovirus removal using trimer and biased hexamer peptides

Biotechnology Journal, 7(4), 558–565.

By: C. Heldt n, P. Gurgel n, L. Jaykus n & R. Carbonell n

author keywords: Affinity adsorption; Biosafety; Bioseparation; Ligand; Virus removal
MeSH headings : Adsorption; Chromatography, Affinity; Humans; Parvovirus, Porcine / chemistry; Parvovirus, Porcine / isolation & purification; Peptides / chemical synthesis; Peptides / chemistry; Plasma / chemistry; Plasma / virology
TL;DR: This study demonstrates that trimeric and hexameric peptides may have different, matrix‐specific roles to play in virus removal applications, and in general, the hexamer ligand may perform better for binding of specific viruses, whereas the trimer ligand might have more broadly reactive virus‐binding properties. (via Semantic Scholar)
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Sources: Web Of Science, Crossref
Added: August 6, 2018

2010 journal article

Binding site on human immunoglobulin G for the affinity ligand HWRGWV

Journal of Molecular Recognition, 23(3), 271–282.

By: H. Yang, P. Gurgel, D. Williams, B. Bobay, J. Cavanagh, D. Muddiman , R. Carbonell

Source: NC State University Libraries
Added: August 6, 2018

2010 journal article

Performance of hexamer peptide ligands for affinity purification of immunoglobulin G from commercial cell culture media

Journal of Chromatography A, 1218(13), 1691–1700.

By: A. Naik n, S. Menegatti n , P. Gurgel n & R. Carbonell n

author keywords: Hexapeptide ligand; IgG purification; Monoclonal antibody; Affinity chromatography; Regeneration
MeSH headings : Adsorption; Animals; Antibodies, Monoclonal / chemistry; Antibodies, Monoclonal / isolation & purification; CHO Cells; Caprylates; Cell Culture Techniques; Chromatography, Affinity / methods; Cricetinae; Cricetulus; Culture Media; Electrophoresis, Polyacrylamide Gel; Humans; Immunoglobulin G / chemistry; Immunoglobulin G / isolation & purification; Oligopeptides / chemistry; Phosphoric Acids; Protein Binding; Sodium Chloride; Urea
TL;DR: Issues related to the successful application of the hexapeptide ligands, on a commercial methacrylate chromatographic resin, for the purification of IgG from mammalian cell culture fluids are addressed. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 article

Identification of trimeric peptides that bind porcine parvovirus from mixtures containing human blood plasma

BIOTECHNOLOGY PROGRESS, Vol. 24, pp. 554–560.

By: C. Heldt n, P. Gurgel n, L. Jaykus n & R. Carbonell n

MeSH headings : Animals; Complex Mixtures / isolation & purification; Complex Mixtures / metabolism; Humans; Oligopeptides / metabolism; Parvovirus, Porcine / isolation & purification; Parvovirus, Porcine / metabolism; Plasma / metabolism; Plasma / virology
TL;DR: The results of this work indicate that small peptide ligand resins have the potential to be used in virus removal processes where removal of contaminating virus is necessary to ensure product safety. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2008 patent

Prion protein binding materials and methods of use

Washington, DC: U.S. Patent and Trademark Office.

By: R. Carbonell, H. Shen, P. Gurgel, V. Wiltshire-Lyerly, D. Hammond & S. Burton

Source: NC State University Libraries
Added: August 6, 2018

2008 journal article

Purification of human immunoglobulin G via Fc-specific small peptide ligand affinity chromatography

JOURNAL OF CHROMATOGRAPHY A, 1216(6), 910–918.

By: H. Yang n, P. Gurgel n & R. Carbonell n

author keywords: Human immunoglobulin G; Fc fragment; Hexamer peptide ligand; Affinity chromatography; Ligand density
MeSH headings : Adsorption; Chromatography, Affinity / methods; Culture Media; Humans; Immobilized Proteins / chemistry; Immobilized Proteins / metabolism; Immunoglobulin Fc Fragments / metabolism; Immunoglobulin G / isolation & purification; Immunoglobulin G / metabolism; Kinetics; Ligands; Peptides / chemistry; Peptides / metabolism; Temperature
TL;DR: The results indicate that these small peptide ligands, especially HWRGWV, offer a potential alternative to the use of Protein A or Protein G for large scale affinity chromatography. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2006 journal article

A colorimetric assay for viral agents that produce cytopathic effects

Journal of Virological Methods, 135(1), 56–65.

By: C. Heldt, R. Hernandez, U. Mudiganti, P. Gurgel, D. Brown & R. Carbonell

Source: NC State University Libraries
Added: August 6, 2018

2006 journal article

Reduction in infectivity of endogenous transmissible spongiform encephalopathies present in blood by adsorption to selective affinity resins

LANCET, 368(9554), 2226–2230.

By: L. Gregori *, P. Gurgel*, J. Lathrop*, P. Edwardson, B. Lambert*, R. Carbonell n, S. Burton, D. Hammond*, R. Rohwer*

MeSH headings : Adsorption; Animals; Cricetinae; Filtration / methods; Prion Diseases / prevention & control; Prion Diseases / transmission; Prions / blood; Prions / isolation & purification; Prions / pathogenicity; Transfusion Reaction
TL;DR: Results showed removal of endogenous TSE infectivity from leucoreduced whole blood by affinity ligands, which can be used to mitigate the risks from TSE-infected blood, blood products, and other materials exposed to TSEinfectivity. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Reduction of transmissible spongiform encephalopathy infectivity from human red blood cells with prion protein affinity ligands

TRANSFUSION, 46(7), 1152–1161.

By: L. Gregori  n, B. Lambert n, P. Gurgel n, L. Gheorghiu n, P. Edwardson n, J. Lathrop n, C. MacAuley n, R. Carbonell n ...

MeSH headings : Animals; Combinatorial Chemistry Techniques; Cricetinae; Erythrocyte Transfusion / methods; Erythrocyte Transfusion / standards; Erythrocytes / chemistry; Humans; Ligands; Models, Biological; Prion Diseases / prevention & control; Prions / blood; Prions / isolation & purification; Protein Binding; Titrimetry
TL;DR: Blood‐borne TSE infectivity cannot be detected with a diagnostic test, nor is it likely to be amenable to inactivation; however, its depletion with specific adsorp‐tive ligand resins is possible. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2005 journal article

Hexamer peptide affinity resins that bind the Fc region of human immunoglobulin G

JOURNAL OF PEPTIDE RESEARCH, 66, 120–137.

By: H. Yang n, P. Gurgel n & R. Carbonell n

author keywords: affinity chromatography; Fc fragment; hexapeptide; immunoglobulin
TL;DR: It was found that HWRGWV possessed the potential to purify HIgG from complete mammalian cell culture medium containing 10% fetal calf serum with purity comparable to commercially available resins, but using milder elution conditions. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2005 article

Small Peptide Ligands for Affinity Separations of Biological Molecules

CHEMICAL ENGINEERING TRENDS AND DEVELOPMENTS, pp. 63–83.

By: G. Wang  n, J. Salm n, P. Gurgel n & R. Carbonell n

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Source: Web Of Science
Added: August 6, 2018

2001 journal article

Identification of peptide ligands generated by combinatorial chemistry that bind alpha-lactalbumin

SEPARATION SCIENCE AND TECHNOLOGY, 36(11), 2411–2431.

By: P. Gurgel n, R. Carbonell n & H. Swaisgood n

TL;DR: The identification of the hexapeptide WHWRKR, obtained from a combinatorial library, that shows affinity for α-lactalbumin is described. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2001 journal article

Studies of the binding of alpha-lactalbumin to immobilized peptide ligands

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 49(12), 5765–5770.

By: P. Gurgel n, R. Carbonell n & H. Swaisgood n

author keywords: alpha-lactalbumin; peptide ligands; bioselective adsorption; binding mechanism
MeSH headings : Adsorption; Amino Acid Sequence; Lactalbumin / metabolism; Ligands; Mutagenesis, Site-Directed; Oligopeptides / metabolism; Protein Binding; Protein Precursors / metabolism; Recombinant Proteins / metabolism; Resins, Plant; Thermodynamics
TL;DR: The present work investigates the mechanism of binding of α-lactalbumin to the peptide ligand WH WRKR and its variants HWRKR and acetylated WHWRKR immobilized on a polymethacrylate chromatographic resin. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2000 journal article

Fractionation of whey proteins with a hexapeptide ligand affinity resin

BIOSEPARATION, 9(6), 385–392.

By: P. Gurgel n, R. Carbonell n & H. Swaisgood n

author keywords: alpha-lactalbumin; bioselective adsorption; peptide ligands; whey protein isolate
MeSH headings : Animals; Cattle; Chromatography, Affinity / methods; Lactalbumin / isolation & purification; Ligands; Milk Proteins / isolation & purification; Oligopeptides; Resins, Synthetic; Whey Proteins
TL;DR: This study describes two methods for production of α-lactalbumin from whey protein isolate using bioselective adsorption and the combined use of an amino resin followed by the WHWRKR resin produce a highly purified α- lact Albumin (100%). (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

patent

Prion protein binding materials and methods of use

Hammond, D. J., Carbonell, R. G., Shen, H., Gurgel, P. V., Wiltshire-Lyerly, V., & Burton, S. J.

By: D. Hammond, R. Carbonell, H. Shen, P. Gurgel, V. Wiltshire-Lyerly & S. Burton

Source: NC State University Libraries
Added: August 6, 2018

patent

Purification of immunoglobulins using affinity chromatography and peptide ligands

Carbonell, R. G., Yang, H., & Gurgel, P. V. Washington, DC: U.S. Patent and Trademark Office.

By: R. Carbonell, H. Yang & P. Gurgel

Source: NC State University Libraries
Added: August 6, 2018

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