Works (108)

Updated: April 3rd, 2024 20:44

2023 journal article

Distinct dynamics of the nucleolus in response to nutrient availability and during development in the rice blast fungus

MBIO, 9.

By: E. Cho*, S. Lee*, R. Dean n & J. Jeon*

author keywords: nucleolus; filamentous fungi; nutrient availability; fungal development; rice blast
TL;DR: Monitoring the nucleolar marker protein, MoNOP1 tagged with red fluorescent protein, reveals that the dynamics of nucleolus in a model plant pathogenic fungus, Magnaporthe oryzae, is distinct from those of animal and yeast nucleoli under low nutrient availability and during pathogenic development. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: October 10, 2023

2023 journal article

Issue Information

MOLECULAR PLANT PATHOLOGY, 24(9), 1015–1016.

By: R. Dean

Sources: Web Of Science, NC State University Libraries
Added: January 29, 2024

2023 article

Plant Disease Detection Using an Electronic Nose

2023 IEEE SENSORS.

By: E. Sennik n, S. Kinoshita-Millard, Y. Oh n, C. Kafer, R. Dean n & O. Oralkan n

author keywords: VOCs; plants; infection; disease; e-nose; machine learning
TL;DR: Experimental results demonstrate the effectiveness of the proposed approach in achieving high accuracy for plant disease detection at the end of the 11th day after plant inoculation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: February 12, 2024

2023 article

Tissues and mechanisms associated with Verticillium wilt resistance in tomato using bi-grafted near-isogenic lines

Oh, Y., Ingram, T., Shekasteband, R., Adhikari, T., Louws, F. J., & Dean, R. A. (2023, May 15). (M. Höfte, Ed.). JOURNAL OF EXPERIMENTAL BOTANY, Vol. 5.

By: Y. Oh n, T. Ingram n, R. Shekasteband n, T. Adhikari n, F. Louws n & R. Dean n

Contributors: Y. Oh n, T. Ingram n, R. Shekasteband n, T. Adhikari n, F. Louws n & R. Dean n

Ed(s): M. Höfte

author keywords: Defense response; gene expression; grafting; secreted proteins; tomato; Verticillium dahliae
TL;DR: These findings advance knowledge of molecular mechanisms underlying the tomato–V. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: May 16, 2023

2022 journal article

Host induced gene silencing of Magnaporthe oryzae by targeting pathogenicity and development genes to control rice blast disease

FRONTIERS IN PLANT SCIENCE, 13.

By: M. Wang n & R. Dean n

author keywords: rice blast; host-induced gene silencing; transgenic plant; RNA silencing; cross-kingdom communication
TL;DR: RNA silencing signals can be transferred from host to an invasive fungus and that HIGS has potential to generate resistant rice against M. oryzae are suggested. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: September 6, 2022

2021 journal article

Electronic nose system based on a functionalized capacitive micromachined ultrasonic transducer (CMUT) array for selective detection of plant volatiles

SENSORS AND ACTUATORS B-CHEMICAL, 341.

By: E. Sennik n, F. Erden n, N. Constantino n, Y. Oh n, R. Dean n & O. Oralkan n

author keywords: CMUT; Electronic nose; Gas sensor; k-NN; Machine learning; Plant disease
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 20, 2021

2021 journal article

Soybean Cyst Nematodes Influence Aboveground Plant Volatile Signals Prior to Symptom Development

FRONTIERS IN PLANT SCIENCE, 12.

By: N. Constantino n, Y. Oh n, E. Sennik n, B. Andersen n, M. Warden*, O. Oralkan n, R. Dean n

author keywords: soybean cyst nematode; VOCs; early disease detection; GC-MS; soybean
TL;DR: It is demonstrated that SCN infestation elevates the release of certain VOCs from foliage and that some are evident prior to symptom development, and may be valuable for innovative diagnostic approaches. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science; OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 26, 2021

2020 journal article

Comparative Genome Analyses of 18 Verticillium dahliae Tomato Isolates Reveals Phylogenetic and Race Specific Signatures

FRONTIERS IN MICROBIOLOGY, 11.

By: T. Ingram n, Y. Oh n, T. Adhikari n, F. Louws n & R. Dean n

author keywords: Solanum lycopersicum; secreted effectors; whole genome resequencing; soilborne fungus; Verticillium dahliae race 2
TL;DR: Four highly clonal lineages/groups were discovered, including a lineage unique to North Carolina isolates, which had the rare MAT1-1 mating type and may suggest that these highly variable regions are enriched in race determinant genes, consistent with the two-speed genome hypothesis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: January 4, 2021

2020 journal article

Comparative Proteomic Analysis of Wild Type and Mutant Lacking an SCF E3 Ligase F-Box Protein in Magnaporthe oryzae

JOURNAL OF PROTEOME RESEARCH, 19(9), 3761–3768.

By: J. Kalmar n, Y. Oh n, R. Dean n & D. Muddiman n

MeSH headings : Ascomycota; F-Box Proteins / genetics; Fungal Proteins / genetics; Magnaporthe / metabolism; Proteomics; Ubiquitin-Protein Ligases / genetics
TL;DR: A label-free quantitative global proteomics technique was used to probe the role of ubiquitination and phosphorylation in the mechanism of how E3 ligase regulates change in virulence of M. oryzae and greatly assist in the selection of future genes for functional studies and enabling mechanistic insight related to virulence. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: October 5, 2020

2020 journal article

Dynamic Changes in the Microbiome of Rice During Shoot and Root Growth Derived From Seeds

FRONTIERS IN MICROBIOLOGY, 11.

By: M. Wang n, A. Eyre n, M. Thon*, Y. Oh n & R. Dean n

author keywords: rice; seed and seedling; microbiome; diversity; driving factors
TL;DR: The microbiome in rice seedlings derived directly from seeds was identified, characterized and compared to the microbiome of the seed, finding greater abundance and diversity were detected for the microbiome associated with roots compared to shoots and with more epiphytes than endophytes. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: October 19, 2020

2020 review

Movement of small RNAs in and between plants and fungi

[Review of ]. MOLECULAR PLANT PATHOLOGY, 21(4), 589–601.

By: M. Wang n & R. Dean n

author keywords: cross kingdom; extracellular vesicles; small RNAs; transportation
MeSH headings : Extracellular Vesicles / metabolism; Fungi / metabolism; Phloem / metabolism; Plasmodesmata / metabolism; RNA Interference; RNA, Plant / metabolism
TL;DR: Evidence for transport through naked form, combined with RNA‐binding proteins or enclosed by vesicles is described, and bidirectional cross‐kingdom communication of small RNAs between host plants and adapted fungal pathogens to affect the outcome of infection is supported. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: February 17, 2020

2019 journal article

Identification and Characterization of the Core Rice Seed Microbiome

PHYTOBIOMES JOURNAL, 3(2), 148–157.

By: A. Eyre n, M. Wang n, Y. Oh n & R. Dean n

author keywords: endophytes; metagenomics; microbiome; microorganism
TL;DR: These core members represent valuable candidates for manipulating the rice microbiome, decreasing the use of chemicals while increasing plant performance. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: May 26, 2020

2019 journal article

Investigating host-pathogen meta-metabolic interactions of Magnaporthe oryzae infected barley using infrared matrix-assisted laser desorption electrospray ionization mass spectrometry

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 412(1), 139–147.

By: J. Kalmar n, Y. Oh n, R. Dean n & D. Muddiman n

author keywords: IR-MALDESI; Mass spectrometry imaging; Orbitrap; Magnaporthe oryzae; Image recognition; Polarity switching
MeSH headings : Hordeum / metabolism; Hordeum / microbiology; Host-Pathogen Interactions; Infrared Rays; Magnaporthe / metabolism; Magnaporthe / physiology; Metabolomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR: Serotonin, a barley defense metabolite, was a compound identified in both positive and negative modes, and metabolites in the melanin pathway, important for infection development of M. oryzae were identified but were unable to be confirmed with MS/MS due to their low abundances. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: December 9, 2019

2018 article

Characterizing Small RNAs in Filamentous Fungi Using the Rice Blast Fungus, Magnaporthe oryzae, as an Example

PLANT PATHOGENIC FUNGI AND OOMYCETES: METHODS AND PROTOCOLS, Vol. 1848, pp. 53–66.

By: V. Raman*, B. Meyers*, R. Dean n & N. Donofrio*

author keywords: sRNAs; Small interfering RNAs; CPA-sRNAs; RNAseq; Fungus
MeSH headings : Computational Biology / methods; Gene Library; Magnaporthe / genetics; Oryza / microbiology; Plant Diseases / microbiology; RNA, Fungal; RNA, Small Untranslated; Software; User-Computer Interface
TL;DR: Protocols for comparative analysis of sRNAs in different conditions, comparisons of sRNA libraries to RNAseq data and identification and analysis of methylguanosine-capped and polyadenylated s RNAs (CPA-sRNAs) are presented. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: May 13, 2019

2018 article

Host-Microbe Interactions: Fungi Vol 46

Dean, R. A., & Rouxel, T. (2018, December). CURRENT OPINION IN MICROBIOLOGY, Vol. 46, pp. III-V.

By: R. Dean n & T. Rouxel*

MeSH headings : Apicomplexa / genetics; Apicomplexa / physiology; Fungi / genetics; Fungi / physiology; Host-Pathogen Interactions; Plant Diseases / genetics; Plant Diseases / immunology; Plant Diseases / microbiology; Protozoan Infections / parasitology; Protozoan Infections / physiopathology; Protozoan Proteins / genetics; Protozoan Proteins / metabolism
Sources: Web Of Science, NC State University Libraries
Added: January 21, 2019

2018 article

Sequential Phosphopeptide Enrichment for Phosphoproteome Analysis of Filamentous Fungi: A Test Case Using Magnaporthe oryzae

PLANT PATHOGENIC FUNGI AND OOMYCETES: METHODS AND PROTOCOLS, Vol. 1848, pp. 81–91.

By: Y. Oh n, W. Franck n & R. Dean n

author keywords: Mass spectrometry; Phosphopeptide enrichment; Magnaporthe oryzae; Iron-IMAC; TiO2; Fungus
MeSH headings : Chromatography, Affinity; Chromatography, Liquid; Computational Biology / methods; Data Analysis; Fungal Proteins; Humans; Magnaporthe / metabolism; Phosphopeptides / chemistry; Phosphopeptides / isolation & purification; Phosphopeptides / metabolism; Phosphoproteins / chemistry; Phosphoproteins / isolation & purification; Phosphoproteins / metabolism; Proteome; Proteomics / methods; Tandem Mass Spectrometry; Titanium / chemistry
TL;DR: An effective protocol for phosphopeptide enrichment using an Iron-IMAC resin in combination with titanium dioxide (TiO2) beads from trypsin digested protein samples of the filamentous fungus Magnaporthe oryzae is described. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: May 13, 2019

2018 review

To B or not to B: a tale of unorthodox chromosomes

[Review of ]. CURRENT OPINION IN MICROBIOLOGY, 46, 50–57.

By: J. Soyer*, M. Balesdent*, T. Rouxel* & R. Dean n

MeSH headings : Chromosomes, Fungal / genetics; Fungal Proteins / genetics; Fungal Proteins / metabolism; Fungi / genetics; Fungi / metabolism; Fungi / pathogenicity; Virulence
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 21, 2019

2017 journal article

Comparative proteomic analysis between nitrogen supplemented and starved conditions in Magnaporthe oryzae

Proteome Science, 15.

By: Y. Oh, S. Robertson, J. Parker, D. Muddiman & R. Dean

Source: NC State University Libraries
Added: August 6, 2018

2016 journal article

Comparative genome analysis and genome evolution of members of the magnaporthaceae family of fungi

BMC GENOMICS, 17.

By: L. Okagaki n, J. Sailsbery n, A. Eyre n & R. Dean n

author keywords: Magnaporthaceae; Magnaporthe; Gaeumannomyces; Two-speed genome; Zig zag model; Comparative genomics; CAzymes; Transcription factors; Diversifying selection; Purifying selection
MeSH headings : Ascomycota / classification; Ascomycota / genetics; Biological Evolution; Comparative Genomic Hybridization; Fungal Proteins / genetics; Genome, Fungal; Magnaporthe / genetics; Multigene Family; Phylogeny; Proteome; Species Specificity; Transcription Factors / genetics
TL;DR: Data show that at the genome level, there is no evidence to suggest multi-speed genome evolution or that proximity to repetitive elements play a role in diversification of genes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

Examining ubiquitinated peptide enrichment efficiency through an epitope labeled protein

ANALYTICAL BIOCHEMISTRY, 512, 114–119.

By: J. Parker n, Y. Oh n, Y. Moazami n, J. Pierce n, P. Loziuk n, R. Dean n, D. Muddiman n

author keywords: Ubiquitination; Gly-Gly enrichment; Enrichment efficiency
MeSH headings : Antibodies, Monoclonal, Murine-Derived / chemistry; Epitopes / chemistry; Muramidase / chemistry; Ubiquitinated Proteins / chemistry; Ubiquitination
TL;DR: It is shown that when conjugated to a lysine containing protein, such as lysozyme, it can be applied as a standard protein to examine ubiquitinated peptide enrichment within a complex background. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 review

Form and function of fungal and oomycete effectors

[Review of ]. FUNGAL BIOLOGY REVIEWS, 30(2), 62–73.

By: W. Sharpee n & R. Dean n

author keywords: Effector; Effectoromics; MAMP-Triggered immunity (MTI); Reactive oxygen species (ROS); Systemic acquired resistance (SAR)
TL;DR: Effectoromics research has led to the identification of a number of effectors with redundant function, indicating that pathogenic fungi and oomycetes contain effectors that are individually dispensable but functionally redundant that act synergistically to promote disease. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

Identification and characterization of suppressors of plant cell death (SPD) effectors from Magnaporthe oryzae

MOLECULAR PLANT PATHOLOGY, 18(6), 850–863.

By: W. Sharpee n, Y. Oh n, M. Yi*, W. Franck n, A. Eyre n, L. Okagaki n, B. Valent*, R. Dean n

author keywords: effector; rice blast; suppressor
MeSH headings : Fungal Proteins / genetics; Fungal Proteins / metabolism; Host-Pathogen Interactions; Magnaporthe / metabolism; Magnaporthe / pathogenicity; Plant Diseases / genetics; Plant Diseases / microbiology; Plant Leaves / metabolism; Plant Leaves / microbiology; Tobacco / metabolism; Tobacco / microbiology
TL;DR: A series of effectors that probably act at different stages of the infection process and contribute to the virulence of M. oryzae are identified, some of which were previously unknown or whose function was unknown. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 article

The influence of funding sources on the scientific method

Okagaki, L. H., & Dean, R. A. (2016, June). MOLECULAR PLANT PATHOLOGY, Vol. 17, pp. 651–653.

By: L. Okagaki n & R. Dean n

MeSH headings : Evolution, Molecular; Financial Management; Genome, Plant / genetics; Models, Theoretical; Research / economics; Science / education; Science / standards
TL;DR: Large‐scale bioinformatics and ‘‐omics’ projects should be viewed as a ‘good investment’ during grant funding, because they provide data that can be mined by an entire field for many years. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Genome sequences of three phytopathogenic species of the Magnaporthaceae family of fungi

G3-Genes Genomes Genetics, 5(12), 2539–2545.

By: L. Okagaki, C. Nunes, J. Sailsbery, B. Clay, D. Brown, T. John, Y. Oh, N. Young ...

Source: NC State University Libraries
Added: August 6, 2018

2015 journal article

Genome-wide profiling of DNA methylation provides insights into epigenetic regulation of fungal development in a plant pathogenic fungus, Magnaporthe oryzae

SCIENTIFIC REPORTS, 5.

By: J. Jeon*, J. Choi*, G. Lee*, S. Park*, A. Huh*, R. Dean n, Y. Lee*

MeSH headings : Animals; DNA (Cytosine-5-)-Methyltransferases / classification; DNA (Cytosine-5-)-Methyltransferases / genetics; DNA (Cytosine-5-)-Methyltransferases / metabolism; DNA Methylation; DNA Transposable Elements / genetics; Epigenesis, Genetic; Fungal Proteins / classification; Fungal Proteins / genetics; Fungal Proteins / metabolism; Gene Expression Profiling; Genome, Fungal; Humans; Magnaporthe / genetics; Phylogeny; Plants / microbiology; Reproduction, Asexual; Sequence Analysis, RNA; Transcription, Genetic
TL;DR: It is found that DNA methylation occurs in and around genes as well as transposable elements and undergoes global reprogramming during fungal development, suggesting that it may have acquired new roles other than controlling the proliferation of TEs. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Phosphoproteome Analysis Links Protein Phosphorylation to Cellular Remodeling and Metabolic Adaptation during Magnaporthe oryzae Appressorium Development

Journal of Proteome Research, 14(6), 2408–2424.

By: W. Franck n, E. Gokce n, S. Randall n, Y. Oh n, A. Eyre n, D. Muddiman n, R. Dean n

author keywords: Magnaporthe oryzae; quantitative phosphoproteomics; appressorium formation; network analysis; transcription factors
MeSH headings : Adaptation, Physiological; Basic Helix-Loop-Helix Transcription Factors / metabolism; Chromatography, Liquid; Fungal Proteins / metabolism; Magnaporthe / metabolism; Magnaporthe / physiology; Oryza / microbiology; Phosphoproteins / metabolism; Phosphorylation; Proteomics; Signal Transduction; Tandem Mass Spectrometry
TL;DR: Network analysis incorporating regulation from transcriptomic, proteomic, and phosphoproteomic data revealed new insights into the regulation of the metabolism of conidial storage reserves and phospholipids, autophagy, actin dynamics, and cell wall metabolism during appressorium formation. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2014 journal article

Mobile elements and mitochondrial genome expansion in the soil fungus and potato pathogen Rhizoctonia solani AG-3

FEMS MICROBIOLOGY LETTERS, 352(2), 165–173.

By: L. Losada*, S. Pakala*, N. Fedorova*, V. Joardar*, S. Shabalina*, J. Hostetler*, S. Pakala*, N. Zafar* ...

author keywords: Basidiomycota; homing endonucleases; repetitive elements
MeSH headings : DNA, Fungal / chemistry; DNA, Fungal / genetics; DNA, Mitochondrial / chemistry; DNA, Mitochondrial / genetics; Evolution, Molecular; Gene Expression Profiling; Genome, Mitochondrial / genetics; Interspersed Repetitive Sequences; Molecular Sequence Data; Rhizoctonia / genetics; Sequence Analysis, DNA
TL;DR: RNA-Seq expression profiling confirmed that the majority of homing endonuclease genes and hypothetical genes are transcriptionally active, and comparative analysis suggests that the mitochondrial genome of R. solani is an example of a dynamic history of expansion in filamentous fungi. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

Experimental Evolution Reveals Genome-Wide Spectrum and Dynamics of Mutations in the Rice Blast Fungus, Magnaporthe oryzae

PLOS ONE, 8(5).

MeSH headings : Biological Evolution; Chromosomes, Fungal; Genome, Fungal; Magnaporthe / genetics; Magnaporthe / pathogenicity; Mutation; Oryza / microbiology; Phenotype; Spores, Fungal; Virulence / genetics
TL;DR: It is found that mutations accumulate rapidly prior to visible phenotypic changes and that both genetic drift and selection seem to contribute to shaping mutational landscape, suggesting the buffering capacity of fungal genome against mutations. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

Temporal Analysis of theMagnaporthe OryzaeProteome During Conidial Germination and Cyclic AMP (cAMP)-mediated Appressorium Formation

Molecular & Cellular Proteomics, 12(8), 2249–2265.

By: W. Franck n, E. Gokce n, Y. Oh n, D. Muddiman n & R. Dean n

MeSH headings : Cyclic AMP / metabolism; Fungal Proteins / metabolism; Magnaporthe / growth & development; Magnaporthe / metabolism; Mitochondrial Proteins / metabolism; Peptide Hydrolases / metabolism; Proteome; Spores, Fungal / growth & development; Spores, Fungal / metabolism
TL;DR: The data presented here provide the first comprehensive view of the M. oryzae proteome during early infection-related development and highlight biological processes important for pathogenicity. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2012 journal article

Comparative Analysis of the Genomes of Two Field Isolates of the Rice Blast Fungus Magnaporthe oryzae

PLOS GENETICS, 8(8).

MeSH headings : DNA Transposable Elements; Fungal Proteins; Gene Duplication; Genome, Fungal; Magnaporthe / classification; Magnaporthe / genetics; Magnaporthe / isolation & purification; Magnaporthe / pathogenicity; Molecular Sequence Data; Multigene Family; Oryza / microbiology; Plant Diseases / microbiology; Repetitive Sequences, Nucleic Acid; Sequence Analysis, DNA; Virulence
TL;DR: The results indicate that gain or loss of unique genes, DNA duplication, gene family expansion, and frequent translocation of transposon-like elements are important factors in genome variation of the rice blast fungus. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

In-Depth Analysis of the Magnaporthe oryzae Conidial Proteome

JOURNAL OF PROTEOME RESEARCH, 11(12), 5827–5835.

By: E. Gokce n, W. Franck n, Y. Oh n, R. Dean n & D. Muddiman n

author keywords: proteomics; mass spectrometry; Magnaporthe oryzae; FASP; statistical tests; differential protein expression
MeSH headings : Cell Nucleus / chemistry; Chemical Fractionation / methods; Chromatography, Liquid / methods; Cytoplasm / chemistry; Fungal Proteins / analysis; Fungal Proteins / chemistry; Magnaporthe / chemistry; Mass Spectrometry / methods; Nanotechnology; Nuclear Proteins / analysis; Nuclear Proteins / chemistry; Peptides / analysis; Peptides / chemistry; Proteome / analysis; Proteome / chemistry; Reproducibility of Results; Sensitivity and Specificity; Spores, Fungal / chemistry; Time Factors
TL;DR: The identification of 2912 conidial proteins at a 1% protein false discovery rate (FDR) is reported and the most extensive study performed on M. oryzae conidia to date is presented, suggesting the detected proteins build a representative portion of the predicted proteome. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Polyubiquitin Is Required for Growth, Development and Pathogenicity in the Rice Blast Fungus Magnaporthe oryzae

PLoS ONE, 7(8), e42868.

By: Y. Oh n, W. Franck n, S. Han*, A. Shows n, E. Gokce n, D. Muddiman n, R. Dean n

Ed(s): K. Nielsen

MeSH headings : Boronic Acids / pharmacology; Bortezomib; Cluster Analysis; Fungal Proteins / genetics; Fungal Proteins / metabolism; Gene Expression Profiling; Gene Expression Regulation, Fungal; Germination / genetics; Magnaporthe / genetics; Magnaporthe / growth & development; Magnaporthe / pathogenicity; Mutation; Nitrogen / metabolism; Oryza / microbiology; Plant Diseases / microbiology; Polyubiquitin / genetics; Polyubiquitin / metabolism; Proteasome Inhibitors / pharmacology; Proteolysis / drug effects; Proteomics; Pyrazines / pharmacology; Ubiquitination
TL;DR: This study suggests that ubiquitination of target proteins plays an important role in nutrient assimilation, development and pathogenicity of M. oryzae. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2012 review

The Top 10 fungal pathogens in molecular plant pathology

[Review of ]. MOLECULAR PLANT PATHOLOGY, 13(4), 414–430.

By: R. Dean n, J. Van Kan*, Z. Pretorius*, K. Hammond-Kosack*, A. Di Pietro*, P. Spanu*, J. Rudd*, M. Dickman* ...

MeSH headings : Fungi / physiology; Fungi / ultrastructure; Plant Pathology; Plants / genetics; Plants / microbiology
TL;DR: A short resumé of each fungus in the Top 10 list and its importance is presented, with the intent of initiating discussion and debate amongst the plant mycology community, as well as laying down a bench-mark. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Characterization and application of small RNAs and RNA silencing mechanisms in fungi

Fungal Biology Reviews, 25(4), 172–180.

By: C. Nunes n, J. Sailsbery n & R. Dean n

TL;DR: This review summarizes important classes of eukaryotic small RNAs and provides a current analysis of the RNA silencing machinery based on available fungal genome sequences and discusses opportunities for exploiting knowledge of smallRNAs andRNA silencing for practical application such as engineering plants resistant to fungal pathogens. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2011 journal article

Comparison of stable-isotope labeling with amino acids in cell culture and spectral counting for relative quantification of protein expression

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 25(17), 2524–2532.

By: T. Collier n, S. Randall n, P. Sarkar n, B. Rao n, R. Dean n & D. Muddiman n

MeSH headings : Amino Acid Sequence; Amino Acids / chemistry; Amino Acids / metabolism; Animals; Cell Differentiation; Cell Line; Ectoderm; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Humans; Isotope Labeling / methods; Mass Spectrometry; Mice; Molecular Sequence Data; Peptide Fragments; Peptide Mapping; Proteins / analysis; Proteins / chemistry; Proteins / metabolism; Proteomics / methods; Sensitivity and Specificity; Tandem Mass Spectrometry; Time Factors
TL;DR: The comparison of stable-isotope labeling of amino acids in cell culture (SILAC) with spectral counting for the quantification of human embryonic stem cells as they differentiate toward the trophectoderm at three time points is presented. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Diverse and tissue-enriched small RNAs in the plant pathogenic fungus, Magnaporthe oryzae

BMC Genomics, 12.

By: C. Nunes n, M. Gowda n, J. Sailsbery n, M. Xue n, F. Chen*, D. Brown n, Y. Oh n, T. Mitchell*, R. Dean n

MeSH headings : Base Sequence; DNA, Intergenic / genetics; Gene Expression Profiling; Hyphae / genetics; Magnaporthe / genetics; Magnaporthe / physiology; Molecular Sequence Data; Plants / microbiology; RNA, Fungal / genetics; RNA, Small Untranslated / genetics; RNA, Transfer / genetics; Repetitive Sequences, Nucleic Acid / genetics; Sequence Analysis, RNA
TL;DR: The authors' findings suggest M. oryzae small RNAs differentially accumulate in vegetative and specialized-infection tissues and may play an active role in genome integrity and regulating growth and development. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2011 journal article

Evaluation of Normalization Methods on GeLC-MS/MS Label-Free Spectral Counting Data to Correct for Variation during Proteomic Workflows

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 22(12), 2199–2208.

By: E. Gokce n, C. Shuford n, W. Franck n, R. Dean n & D. Muddiman n

author keywords: Label-free; Proteomics; Mass spectrometry; Total spectral count normalization; Normalized spectral abundance factor; Quantification; Spectral counting; Significance test; Fold-change; GeLC-MS/MS; nanoLC-MS; Orbitrap XL
TL;DR: The correlation between SpCs of the same proteins across the different data sets was investigated and it was reported that TSpC normalization and NSAF normalization yielded almost ideal slopes of unity for normalized SpC versus average normalized SpCs, while NSP did not afford effective corrections of the unnormalized data. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 review

Host-induced gene silencing: a tool for understanding fungal host interaction and for developing novel disease control strategies

[Review of ]. MOLECULAR PLANT PATHOLOGY, 13(5), 519–529.

By: C. Nunes n & R. Dean n

MeSH headings : Fungi / genetics; Gene Silencing / physiology; Host-Pathogen Interactions; Plant Diseases / microbiology; Plants / microbiology
TL;DR: Important aspects for the development of fungal-derived resistance through the expression of silencing constructs in host plants as a powerful strategy to control fungal disease are addressed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Improving Proteome Coverage on a LTQ-Orbitrap Using Design of Experiments

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 22(4), 773–783.

By: G. Andrews n, R. Dean n, A. Hawkridge n & D. Muddiman n

author keywords: LTQ-Orbitrap XL MS/MS instrument parameters; Design of experiments; Fractional-factorial design; Saccharomyces cerevisiae; Proteomics
MeSH headings : Peptide Fragments; Peptide Mapping; Proteome / chemistry; Proteomics / methods; Research Design; Saccharomyces cerevisiae Proteins / chemistry; Tandem Mass Spectrometry / methods; Temperature
TL;DR: The DOE approach represents a time- and cost-effective method for empirically optimizing MS-based proteomics workflows including sample preparation, LC conditions, and multiple instrument platforms. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Increasing proteome coverage with offline RP HPLC coupled to online RP nanoLC-MS

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 879(9-10), 610–614.

By: E. Gokce n, G. Andrews n, R. Dean n & D. Muddiman n

author keywords: RP HPLC; Fractionation methods; Proteomics; Mass spectrometry
MeSH headings : Chromatography, High Pressure Liquid / methods; Chromatography, Reverse-Phase / methods; Mass Spectrometry / methods; Nanotechnology; Peptide Fragments / analysis; Peptide Fragments / metabolism; Proteome / analysis; Proteome / metabolism; Proteomics / methods; Saccharomyces cerevisiae Proteins / analysis; Saccharomyces cerevisiae Proteins / metabolism
TL;DR: The success of performing offline reversed phase high pressure liquid chromatography (HPLC) fractionation on a C18 2.0 mm×150 mm column at the peptide level with microliter per minute flow rates prior to online nano-flow reversed phase liquid Chromatography mass spectrometry (nanoLC-MS) using the well-studied fungus Saccharomyces cerevisiae is reported. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

MoSfl1 Is Important for Virulence and Heat Tolerance in Magnaporthe oryzae

PLOS ONE, 6(5).

MeSH headings : Adaptation, Physiological; Genes, Fungal; Hot Temperature; Magnaporthe / genetics; Magnaporthe / pathogenicity; Magnaporthe / physiology; Virulence
TL;DR: The formation of appressoria, specialized plant penetration structures of Magnaporthe oryzae, is regulated by the MST11-MST7-PMK1 MAP kinase cascade and the SFL1 orthologs are conserved in filamentous ascomycetes but none of them have been functionally characterized in non-Saccharomycetales fungi. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Phylogenetic Analysis and Classification of the Fungal bHLH Domain

MOLECULAR BIOLOGY AND EVOLUTION, 29(5), 1301–1318.

By: J. Sailsbery n, W. Atchley n & R. Dean n

author keywords: bHLH; fungal; phylogeny; discriminant; analysis
MeSH headings : Amino Acid Sequence; Ascomycota / genetics; Basidiomycota / genetics; Decision Trees; Discriminant Analysis; Fungal Proteins / chemistry; Fungal Proteins / classification; Fungal Proteins / genetics; Genome, Fungal; Helix-Loop-Helix Motifs / genetics; Models, Biological; Molecular Sequence Annotation; Molecular Sequence Data; Phylogeny; Sensitivity and Specificity
TL;DR: The phylogenetic relationship of 490 fungal-specific bHLH containing proteins from 55 whole genome projects composed of 49 Ascomycota and 6 Basidiomycota organisms was obtained and evaluated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Transcriptome profiling of the rice blast fungus during invasive plant infection and in vitro stresses

BMC GENOMICS, 12.

By: S. Mathioni*, A. Belo*, C. Rizzo*, R. Dean n & N. Donofrio*

MeSH headings : Fungal Proteins / genetics; Gene Expression Profiling; Gene Expression Regulation, Fungal / genetics; Gene Expression Regulation, Fungal / physiology; Magnaporthe / genetics; Magnaporthe / growth & development; Magnaporthe / pathogenicity; Oryza / microbiology; Oxidative Stress / physiology; Reverse Transcriptase Polymerase Chain Reaction; Temperature
TL;DR: These findings suggest that M. oryzae is likely primarily coping with nutrient-limited environments at the invasive growth stage 72 hours post-inoculation, and not with oxidative or temperature stresses. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Combining ChIP-chip and Expression Profiling to Model the MoCRZ1 Mediated Circuit for Ca2+/Calcineurin Signaling in the Rice Blast Fungus

PLOS PATHOGENS, 6(5).

MeSH headings : Calcineurin / metabolism; Calcium / metabolism; Fungal Proteins / genetics; Fungal Proteins / metabolism; Gene Expression Profiling; Genome, Fungal; Green Fluorescent Proteins / genetics; Magnaporthe / genetics; Oligonucleotide Array Sequence Analysis; Oryza / microbiology; Plant Diseases / microbiology; Signal Transduction / physiology; Transcription Factors / genetics; Transcription Factors / metabolism
TL;DR: It is proposed that calcium/calcineurin regulated signal transduction circuits controlling development and pathogenicity manifest through multiple layers of regulation. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Direct Comparison of Stable Isotope Labeling by Amino Acids in Cell Culture and Spectral Counting for Quantitative Proteomics

ANALYTICAL CHEMISTRY, 82(20), 8696–8702.

By: T. Collier n, P. Sarkar n, W. Franck n, B. Rao n, R. Dean n & D. Muddiman n

MeSH headings : Amino Acids / analysis; Animals; Cell Line; Humans; Isotope Labeling / methods; Mice; Proteomics / methods
TL;DR: A direct comparison of SILAC to label-free quantification by spectral counting of an identical set of data from the bottom-up proteomic analysis of human embryonic stem cells, finding that both strategies result in a similar number of protein identifications. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 review

Unifying Themes in Microbial Associations with Animal and Plant Hosts Described Using the Gene Ontology

[Review of ]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 74(4), 479–503.

By: T. Torto-Alalibo*, C. Collmer*, M. Gwinn-Giglio*, M. Lindeberg*, S. Meng n, M. Chibucos*, T. Tseng*, J. Lomax* ...

Contributors: T. Torto-Alalibo*, C. Collmer*, M. Gwinn-Giglio*, M. Lindeberg*, S. Meng n, M. Chibucos*, T. Tseng*, J. Lomax* ...

MeSH headings : Animals; Bacteria / genetics; Bacteria / metabolism; Bacterial Physiological Phenomena; Cell Adhesion; Computational Biology / methods; Fungi / genetics; Fungi / metabolism; Fungi / physiology; Host-Pathogen Interactions / genetics; Host-Pathogen Interactions / immunology; Plants / immunology; Plants / microbiology; Protein Transport; Symbiosis / genetics; Symbiosis / immunology; Virulence Factors
TL;DR: Some unifying themes common to diverse host-microbe associations are reviewed and areas where new terms need to be developed are highlighted, an ongoing process that should involve the whole community. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2009 review

Common processes in pathogenesis by fungal and oomycete plant pathogens, described with Gene Ontology terms

[Review of ]. BMC MICROBIOLOGY, 9.

By: S. Meng n, T. Torto-Alalibo*, M. Chibucos*, B. Tyler* & R. Dean n

MeSH headings : Fungi / pathogenicity; Host-Pathogen Interactions; Oomycetes / pathogenicity; Plant Diseases / microbiology; Plants / microbiology; Signal Transduction; Spores / pathogenicity; Terminology as Topic; Vocabulary, Controlled
TL;DR: This mini-review identifies common features of the pathogenic processes of fungi and oomycetes and creates a pathogenesis model using 256 newly developed and 38 extant GO terms, with an emphasis on the appressorium and signal transduction. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 review

Gene Ontology annotation of the rice blast fungus, Magnaporthe oryzae

[Review of ]. BMC MICROBIOLOGY, 9.

By: S. Meng n, D. Brown n, D. Ebbole*, T. Torto-Alalibo*, Y. Oh n, J. Deng n, T. Mitchell n, R. Dean n

MeSH headings : Computational Biology; Databases, Protein; Fungal Proteins / genetics; Genome, Fungal; Magnaporthe / genetics; Oryza / microbiology; Sequence Alignment; Sequence Analysis, Protein; Terminology as Topic; Vocabulary, Controlled
TL;DR: This analysis provides comprehensive and robust GO annotations of the M. oryzae genome assemblies that will be solid foundations for further functional interrogation of M.oryzae. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 review

Infection strategies of filamentous microbes described with the Gene Ontology

[Review of ]. TRENDS IN MICROBIOLOGY, 17(7), 320–327.

By: T. Torto-Alalibo*, S. Meng n & R. Dean n

MeSH headings : Actinomycetales / genetics; Computational Biology / methods; Fungi / genetics; Oomycetes / genetics; Virulence Factors / genetics; Virulence Factors / metabolism; Vocabulary, Controlled
TL;DR: Common strategies employed by filamentous organisms during colonization of their hosts are reviewed, with reference to GO terms that best describe the processes involved, in the absence of sequence similarity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

Altered patterns of gene duplication and differential gene gain and loss in fungal pathogens

BMC GENOMICS, 9.

By: A. Powell*, G. Conant*, D. Brown n, I. Carbone n & R. Dean n

Contributors: A. Powell*, G. Conant*, D. Brown n, I. Carbone n & R. Dean n

MeSH headings : Computational Biology; Evolution, Molecular; Fungi / genetics; Fungi / pathogenicity; Gene Duplication; Genomics; Multigene Family / genetics; Sequence Homology
TL;DR: Differences in the overall levels of gene duplication in phytopathogenic species versus non-pathogenic relatives implicate gene inventory flux as an important virulence-associated process in fungi. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2008 article

Aspergillus flavus genomics as a tool for studying the mechanism of aflatoxin formation

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, Vol. 25, pp. 1152–1157.

By: J. Yu*, G. Payne n, W. Nierman*, M. Machida*, J. Bennett*, B. Campbell*, J. Robens*, D. Bhatnagar*, R. Dean n, T. Cleveland*

author keywords: aflatoxins; mycotoxins; toxigenic fungi; Aspergillus; genomics; expressed Sequence tags; microarrays; gene profiling
MeSH headings : Aflatoxins / biosynthesis; Aspergillus flavus / genetics; Aspergillus flavus / metabolism; Aspergillus oryzae / genetics; Gene Expression Profiling / methods; Genes, Fungal; Genome, Fungal; Genomics / methods; Genomics / trends; Multigene Family; Oligonucleotide Array Sequence Analysis / methods
TL;DR: The major objective of the A. flavus genomics program is the identification of genes involved in aflatoxin biosynthesis and regulation, as well as in pathogenicity, to gain a better understanding of the mechanism of a Flatoxin formation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

The effect of temperature on Natural Antisense Transcript (NAT) expression in Aspergillus flavus

Current Genetics, 54(5), 241–269.

By: C. Smith*, D. Robertson n, B. Yates*, D. Nielsen n, D. Brown n, R. Dean n, G. Payne n

author keywords: Aspergillus flavus; Natural antisense transcripts (NATs); Temperature; Gene expression
MeSH headings : Aspergillus flavus / genetics; Aspergillus flavus / metabolism; Expressed Sequence Tags; Fungal Proteins / genetics; Fungal Proteins / metabolism; Gene Expression Regulation, Fungal; Genome, Fungal; RNA Precursors / metabolism; RNA, Antisense / metabolism; Temperature; Transcription, Genetic / genetics
TL;DR: RNA expression analysis showed that 32 NATs and 2,709 genes were differentially expressed between 37°C and 28°C, the conducive temperature for the biosynthesis of aflatoxin (AF) and many other secondary metabolites. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2008 review

Transcriptome analysis reveals new insight into appressorium formation and function in the rice blast fungus Magnaporthe oryzae

[Review of ]. GENOME BIOLOGY, 9(5).

By: Y. Oh n, N. Donofrio n, H. Pan n, S. Coughlan*, D. Brown n, S. Meng n, T. Mitchell n, R. Dean n

MeSH headings : Cell Wall / chemistry; Cell Wall / metabolism; Fungal Proteins / genetics; Fungal Proteins / metabolism; Gene Expression Profiling; Gene Expression Regulation, Fungal; Lipid Metabolism; Magnaporthe / cytology; Magnaporthe / genetics; Magnaporthe / physiology; Signal Transduction; Spores, Fungal / cytology; Spores, Fungal / genetics
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

A systematic analysis of T-DNA insertion events in Magnaporthe oryzae

FUNGAL GENETICS AND BIOLOGY, 44(10), 1050–1064.

By: Y. Meng*, G. Patel*, M. Heist*, M. Betts, S. Tucker*, N. Galadima*, N. Donofrio n, D. Brown n ...

author keywords: agrobacterium-mediated transformation; insertional mutagenesis; inverse PCR
MeSH headings : 3' Flanking Region; 5' Flanking Region; Agrobacterium tumefaciens / genetics; Chromosomes, Fungal; DNA, Bacterial / analysis; DNA, Bacterial / genetics; Genome, Fungal; Magnaporthe / genetics; Mutagenesis, Insertional; Repetitive Sequences, Nucleic Acid; Transformation, Genetic
TL;DR: Analysis of random T-DNA insertions that were generated as part of a large-scale insertional mutagenesis project for Magnaporthe oryzae found that the distribution of T- DNA inserts among the M.Oryzae chromosomes was not random and significant insertion bias was detected. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Challenges in quantification of ligninolytic enzymes from Phanerochaete chrysosporium cultivation for pretreatment of cotton stalks

Transactions of the ASABE, 50(6), 2347–2354.

By: J. Shi, R. Sharma-Shivappa*, M. Chinn*, R. Dean* & R. Shivappa*

Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2007 journal article

Development of a high throughput transformation system for insertional mutagenesis in Magnaporthe oryzae

FUNGAL GENETICS AND BIOLOGY, 44(10), 1035–1049.

By: M. Betts, S. Tucker*, N. Galadima*, Y. Meng*, G. Patel*, L. Li*, N. Donofrio n, A. Floyd n ...

author keywords: Magnaporthe oryzae; Insertional mutagenesis; Agrobacterium tumefaciens-mediated transformation; CaCl2/PEG-mediated transformation; pathogenicity
MeSH headings : 3' Flanking Region; 5' Flanking Region; Agrobacterium tumefaciens / genetics; Magnaporthe / genetics; Mutagenesis, Insertional / methods; Phenotype; Protoplasts; Transformation, Genetic
TL;DR: A description of the high throughput transformation and analysis pipeline used to create the most comprehensive insertional mutagenesis analysis of a fungal pathogen is provided. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Expressed sequence tags from Phytophthora sojae reveal genes specific to development and infection

MOLECULAR PLANT-MICROBE INTERACTIONS, 20(7), 781–793.

By: T. Torto-Alalibo*, S. Tripathy, B. Smith, F. Arredondo, L. Zhou, H. Li*, M. Chibucos, D. Qutob ...

author keywords: effectors
MeSH headings : Cluster Analysis; Expressed Sequence Tags; Gene Expression Profiling; Gene Library; Genes, Fungal; Molecular Sequence Data; Phytophthora / genetics; Phytophthora / growth & development; Sequence Analysis, DNA; Soybeans / microbiology
TL;DR: Six unique expressed sequence tag libraries were generated from four developmental stages of Phytophthora sojae and revealed genes that were specific to or highly represented in particular growth conditions and life stages, indicating that, during infection, the pathogen derives most of its carbon and energy via glycolysis of sugars in the plant. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Expression of a harpin-encoding gene in rice confers durable nonspecific resistance to Magnaporthe grisea

PLANT BIOTECHNOLOGY JOURNAL, 6(1), 73–81.

By: M. Shao*, J. Wang*, R. Dean n, Y. Lin*, X. Gao* & S. Hu n

author keywords: appressorium; defence-related genes; durable nonspecific resistance; hrf1 gene; Magnaporthe grisea; rice
MeSH headings : Bacterial Outer Membrane Proteins / genetics; Bacterial Outer Membrane Proteins / metabolism; Gene Expression; Host-Pathogen Interactions; Magnaporthe / immunology; Magnaporthe / physiology; Oryza / genetics; Oryza / immunology; Oryza / microbiology; Plant Diseases; Plant Leaves / metabolism; Silicon / metabolism; Xanthomonas / genetics
TL;DR: It is shown that the expression of a harpin-encoding gene (hrf1) confers nonspecific resistance in rice to the blast fungus Magnaporthe grisea, and it is suggested that harpins may offer new opportunities for generating broad-spectrum disease resistance in other crops. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 review

From genes to genomes: A new paradigm for studying fungal pathogenesis in magnaporthe oryzae

[Review of ]. Fungal genomics, 57, 175–218.

By: J. Xu, X. Zhao & R. Dean

Source: NC State University Libraries
Added: August 6, 2018

2007 article

Fungal gene clusters

Dean, R. A. (2007, January). NATURE BIOTECHNOLOGY, Vol. 25, pp. 67–67.

By: R. Dean n

MeSH headings : Fungal Proteins / genetics; Fungal Proteins / metabolism; Gene Expression Profiling; Gene Expression Regulation, Fungal; Genes, Fungal / genetics; Genome, Fungal / genetics; Genomics; Multigene Family / genetics; Multigene Family / physiology; Ustilago / genetics; Ustilago / growth & development; Ustilago / pathogenicity; Virulence / genetics; Zea mays / microbiology
TL;DR: Identifying so-called ‘biotrophy clusters’ may increase understanding of fungal disease just as the discovery of pathogenicity islands did for bacterial infection, and there are several similarities between the novel clusters and pathogenicicity islands. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Magnaporthe grisea infection triggers RNA variation and antisense transcript expression in rice

PLANT PHYSIOLOGY, 144(1), 524–533.

By: M. Gowda n, R. Venu n, H. Li n, C. Jantasuriyarat n, S. Chen n, M. Bellizzi n, V. Pampanwar n, H. Kim n ...

MeSH headings : Base Sequence; Expressed Sequence Tags; Gene Expression Profiling; Gene Library; Genetic Variation; Immunity, Innate; Magnaporthe / physiology; Molecular Sequence Data; Oryza / genetics; Oryza / metabolism; Oryza / microbiology; Plant Diseases / genetics; Plant Diseases / microbiology; RNA, Antisense / metabolism; RNA, Messenger / metabolism; Sequence Analysis, RNA
TL;DR: Rice blast disease, caused by the fungal pathogen Magnaporthe grisea, is an excellent model system to study plant-fungal interactions and host defense responses and robust-long serial analysis of gene expression demonstrated that there is a series of dynamic and complex transcript modifications and changes in the rice transcriptome at the M.grisea early infection stages. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

The Magnaporthe grisea snodprot1 homolog, MSPI, is required for virulence

FEMS MICROBIOLOGY LETTERS, 273(2), 157–165.

By: J. Jeong n, T. Mitchell n & R. Dean n

author keywords: rice blast; hydrophobin; pathogenicity; rice; infection
MeSH headings : Blotting, Western; Fungal Proteins / chemistry; Fungal Proteins / genetics; Fungal Proteins / physiology; Gene Deletion; Hordeum / microbiology; Magnaporthe / genetics; Magnaporthe / metabolism; Magnaporthe / pathogenicity; Molecular Sequence Data; Mutation; Recombinant Fusion Proteins / physiology; Sequence Alignment; Sequence Analysis, Protein; Virulence / genetics; Virulence Factors / chemistry; Virulence Factors / genetics; Virulence Factors / physiology
TL;DR: This is the first report to the authors' knowledge that directly implicates a snodprot1 protein as a virulence factor in the rice blast fungus Magnaporthe grisea. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

The evolutionary history of Cytochrome P450 genes in four filamentous Ascomycetes

BMC EVOLUTIONARY BIOLOGY, 7.

By: J. Deng n, I. Carbone n & R. Dean n

MeSH headings : Amino Acid Motifs; Amino Acid Sequence; Ascomycota / enzymology; Ascomycota / genetics; Aspergillus nidulans / enzymology; Aspergillus nidulans / genetics; Conserved Sequence; Cytochrome P-450 Enzyme System / genetics; Evolution, Molecular; Exons; Fusarium / enzymology; Fusarium / genetics; Gene Duplication; Genes, Fungal; Introns; Magnaporthe / enzymology; Magnaporthe / genetics; Multigene Family; Neurospora crassa / enzymology; Neurospora crassa / genetics; Phylogeny
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Trranscript profiling of a conifer pathosystem: response of Pinus sylvestris root tissues to pathogen (Heterobasidion annosum) invasion

TREE PHYSIOLOGY, 27(10), 1441–1458.

By: A. Adomas*, G. Heller*, G. Li*, A. Olson*, T. Chu*, J. Osborne n, D. Craig n, L. Van Zyl n ...

author keywords: antimicrobial peptide; microarray; peroxidase; phenylopropanoid pathway; pine; plant defence
MeSH headings : Fungi / physiology; Gene Expression Profiling; Gene Expression Regulation, Plant; Oligonucleotide Array Sequence Analysis; Pinus sylvestris / metabolism; Plant Diseases / microbiology; Plant Proteins / genetics; Plant Proteins / metabolism; Plant Roots / metabolism; Plant Roots / microbiology; Plant Roots / ultrastructure; Seedlings
TL;DR: The similarity of the expression profiling pattern observed in this pathosystem to those documented in crop pathology suggests that angiosperms and gymnosperms use similar genetic programs in responding to invasive growth by microbial pathogens. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 article

What can comparative genomics tell us about species concepts in the genus Aspergillus?

STUDIES IN MYCOLOGY, pp. 11–17.

By: A. Rokas*, G. Payne n, N. Fedorova*, S. Baker*, M. Machida*, J. Yu*, D. Georgianna n, R. Dean n ...

author keywords: comparative genomics; genetic diversity; genome sequences; identification of species boundaries
TL;DR: It is argued that genomic data can aid Aspergillus taxonomy by serving as a source of novel and unprecedented amounts of comparative data, as a resource for the development of additional diagnostic tools, and finally as a knowledge database about the biological differences between strains and species. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

Construction of a watermelon BAC library and identification of SSRs anchored to melon or Arabidopsis genomes

THEORETICAL AND APPLIED GENETICS, 112(8), 1553–1562.

By: T. Joobeur n, G. Gusmini*, X. Zhang*, A. Levi*, Y. Xu*, T. Wehner n, M. Oliver, R. Dean n

MeSH headings : Alleles; Arabidopsis / genetics; Chromosomes, Artificial, Bacterial; Citrullus / genetics; DNA, Plant / genetics; Gene Library; Genetic Markers; Genome, Plant; Hybridization, Genetic; Polymorphism, Genetic; Repetitive Sequences, Nucleic Acid / genetics; Sequence Analysis, DNA
TL;DR: The methods described herein will be valuable for the construction of a watermelon linkage map with SSRs evenly distributed on its genome that is anchored to the genomes of melon and Arabidopsis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

Deep and comparative analysis of the mycelium and appressorium transcriptomes of Magnaporthe grisea using MPSS, RL-SAGE, and oligoarray methods

BMC Genomics, 7.

By: M. Gowda, R. Venu, M. Raghupathy, K. Nobuta, H. Li, R. Wing, E. Stahlberg, S. Couglan ...

Source: NC State University Libraries
Added: August 6, 2018

2006 journal article

Global gene expression during nitrogen starvation in the rice blast fungus, Magnaporthe grisea

FUNGAL GENETICS AND BIOLOGY, 43(9), 605–617.

By: N. Donofrio n, Y. Oh n, R. Lundy n, H. Pan n, D. Brown n, J. Jeong n, S. Coughlan*, T. Mitchell n, R. Dean n

author keywords: nitrogen limitation; GATA transcription factors; metabolism; SPM1; protease; targeted gene disruption; pathogenicity
MeSH headings : Amino Acids / metabolism; Binding Sites; Biological Transport / genetics; DNA-Binding Proteins / genetics; GATA Transcription Factors / metabolism; Gene Expression; Gene Expression Regulation, Fungal; Genes, Fungal; Magnaporthe / genetics; Magnaporthe / pathogenicity; Nitrogen / metabolism; Oligonucleotide Array Sequence Analysis; Oryza / metabolism; Oryza / microbiology; Plant Diseases / microbiology; Promoter Regions, Genetic; Serine Endopeptidases / genetics
TL;DR: A genome-wide analysis of gene expression under nitrogen-limiting conditions in the rice blast pathogen Magnaporthe grisea resulted in targeted gene replacement on the vacuolar serine protease, SPM1, resulting in decreased sporulation and appressorial development as well as a greatly attenuated ability to cause disease. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

MGOS: A resource for studying Magnaporthe grisea and Oryza sativa interactions

MOLECULAR PLANT-MICROBE INTERACTIONS, 19(10), 1055–1061.

By: C. Soderlund*, K. Haller*, V. Pampanwar*, D. Ebbole*, M. Farman*, M. Orbach*, G. Wang*, R. Wing* ...

author keywords: host-pathogen
MeSH headings : Computational Biology; DNA, Complementary / chemistry; Databases, Genetic; Expressed Sequence Tags; Gene Expression Profiling; Genome, Fungal; Genome, Plant; Internet; Magnaporthe / genetics; Magnaporthe / physiology; Mutation; Oligonucleotide Array Sequence Analysis; Oryza / genetics; Oryza / microbiology; Phenotype
TL;DR: The MGOS (Magnaporthe grisea Oryza sativa) web-based database contains data from interaction experiments in which M.grisea is the fungal pathogen that causes the rice blast disease and a consortium of fungal and rice geneticists was formed to study the interactions. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 review

Organization of chromosome ends in the rice blast fungus, Magnaporthe oryzae

[Review of ]. NUCLEIC ACIDS RESEARCH, 34(17), 4685–4701.

By: C. Rehmeyer*, W. Li*, M. Kusaba*, Y. Kim*, D. Brown n, C. Staben*, R. Dean n, M. Farman*

MeSH headings : Adenosine Triphosphatases / genetics; Base Sequence; Chromosomes, Fungal / chemistry; DNA Helicases / genetics; DNA Transposable Elements; Fungal Proteins / genetics; Gene Duplication; Genes, Fungal; Magnaporthe / genetics; Magnaporthe / metabolism; Molecular Sequence Data; Oryza / microbiology; RecQ Helicases; Sequence Analysis, DNA; Telomere / chemistry; Terminology as Topic
TL;DR: It is proposed that the M.oryzae chromosome termini play more subtle roles in host adaptation by promoting the loss of terminally-positioned genes that tend to trigger host defenses. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

The role of transposable element clusters in genome evolution and loss of synteny in the rice blast fungus Magnaporthe oryzae

Genome Biology, 7(2).

By: M. Thon, H. Pan, S. Diener, J. Papalas, A. Taro, T. Mitchell, R. Dean

Source: NC State University Libraries
Added: August 6, 2018

2006 article

Whole genome comparison of Aspergillus flavus and A-oryzae

Payne, G. A., Nierman, W. C., Wortman, J. R., Pritchard, B. L., Brown, D., Dean, R. A., … Yu, J. (2006, September). MEDICAL MYCOLOGY, Vol. 44, pp. S9–S11.

By: G. Payne n, W. Nierman, J. Wortman, B. Pritchard n, D. Brown n, R. Dean n, D. Bhatnagar, T. Cleveland, M. Machida*, J. Yu

author keywords: aflatoxin; secondary metabolism; koji moulds
TL;DR: The high degree of sequence similarity between the two fungi suggests that they may be ecotypes of the same species and that A. oryzae has resulted from the domestication of A. flavus. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 journal article

Alkahest NuclearBLAST: a user-friendly BLAST management and analysis system

BMC Bioinformatics, 6.

Source: NC State University Libraries
Added: August 6, 2018

2005 journal article

Genetic variation among Rhizoctonia solani isolates from warm-season turfgrasses

International Turfgrass Society Research Journal, 10, 230.

By: J. Li, S. Martin, S. Jeffers, R. Dean & J. Camberato

Source: NC State University Libraries
Added: August 6, 2018

2005 journal article

Large-scale identification of expressed sequence tags involved in rice and rice blast fungus interaction

PLANT PHYSIOLOGY, 138(1), 105–115.

By: C. Jantasuriyarat n, M. Gowda n, K. Haller n, J. Hatfield n, G. Lu n, E. Stahlberg n, B. Zhou n, H. Li n ...

MeSH headings : Disease Susceptibility; Expressed Sequence Tags; Fungal Proteins / genetics; Gene Library; Genes, Plant; Immunity, Innate; Magnaporthe / genetics; Magnaporthe / pathogenicity; Molecular Sequence Data; Oryza / genetics; Oryza / microbiology; Plant Diseases / genetics; Plant Leaves / microbiology; Plant Proteins / genetics
TL;DR: The large cataloged collection of rice ESTs in this study provides a solid foundation for further characterization of the rice defense response and is a useful public genomic resource for rice functional genomics studies. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 journal article

Novel G-protein-coupled receptor-like proteins in the plant pathogenic fungus Magnaporthe grisea

Genome Biology, 6(3).

By: R. Kulkarni, M. Thon, H. Pan & R. Dean

Source: NC State University Libraries
Added: August 6, 2018

2005 journal article

PACLIMS: A component LIM system for high-throughput functional genomic analysis

BMC Bioinformatics, 6.

By: N. Donofrio, R. Rajagopalon, D. Brown, S. Diener, D. Windham, S. Nolin, A. Floyd, T. Mitchell ...

Source: NC State University Libraries
Added: August 6, 2018

2005 journal article

The genome sequence of the rice blast fungus Magnaporthe grisea

NATURE, 434(7036), 980–986.

MeSH headings : Fungal Proteins / genetics; Fungal Proteins / metabolism; Genes, Fungal / genetics; Genome, Fungal; Guanosine Diphosphate / metabolism; Guanosine Triphosphate / metabolism; Magnaporthe / classification; Magnaporthe / genetics; Magnaporthe / metabolism; Magnaporthe / pathogenicity; Oryza / microbiology; Plant Diseases / microbiology; Point Mutation / genetics; Proteome / genetics; Proteome / metabolism; Receptors, G-Protein-Coupled / genetics; Repetitive Sequences, Nucleic Acid / genetics; Saccharomyces cerevisiae / metabolism; Signal Transduction; Virulence / genetics
TL;DR: The draft sequence of the M. grisea genome is reported, reflecting the clonal nature of this fungus imposed by widespread rice cultivation and analysis of the gene set provides an insight into the adaptations required by a fungus to cause disease. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 chapter

Trust-Based Secure Workflow Path Construction

In B. Benatallah, F. Casati, & P. Traverso (Eds.), Service-Oriented Computing – ICSOC 2007 (pp. 382–395).

By: M. Altunay n, D. Brown n, G. Byrd n & R. Dean n

Contributors: M. Altunay n, D. Brown n, G. Byrd n & R. Dean n

Ed(s): B. Benatallah, F. Casati & P. Traverso

TL;DR: A workflow management framework that fully integrates trust and security into the workflow planning logic, which considers not only trust relationships between the workflow requestor and individual services, but also trust relationships among the services themselves. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2004 journal article

BAC end sequences and a physical map reveal transposable element content and clustering patterns in the genome of Magnaporthe grisea

FUNGAL GENETICS AND BIOLOGY, 41(7), 657–666.

By: M. Thon n, S. Martin n, S. Goff*, R. Wing* & R. Dean n

author keywords: transposon; transposable element; rice blast; Magnaporthe grisea; Pyricularia grisea; BAC library; physical map
MeSH headings : Chromosomes, Artificial, Bacterial; Chromosomes, Fungal / genetics; DNA Transposable Elements; DNA, Fungal / genetics; Gene Library; Genome, Fungal; Magnaporthe / genetics; Physical Chromosome Mapping; Point Mutation / genetics; Recombination, Genetic; Repetitive Sequences, Nucleic Acid; Sequence Analysis, DNA
TL;DR: This study illustrates the general utility of a physical map and BAC end sequences for the study of genome-wide repetitive DNA content and organization. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 article

Characterization of the protein processing and secretion pathways in a comprehensive set of expressed sequence tags from Trichoderma reesei (vol 230, pg 275, 2004)

FEMS Microbiology Letters, Vol. 235, p. 209.

By: S. Diener n, N. Dunn-Coleman, P. Foreman, T. Houfek n, P. Teunissen, P. Solingen, L. Dankmeyer, T. Mitchell n, M. Ward, R. Dean n

Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2004 journal article

Cloning, sequencing and functional analysis of Magnaporthe grisea MVP1 gene, a hex-1 homolog encoding a putative 'woronin body' protein

FEMS Microbiology Letters, 230(1), 85–90.

By: F. Asiegbu, W. Choi, J. Jeong & R. Dean

Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2004 journal article

Gene discovery and gene expression in the rice blast fungus, Magnaporthe grisea: Analysis of expressed sequence tags

MOLECULAR PLANT-MICROBE INTERACTIONS, 17(12), 1337–1347.

By: D. Ebbole*, Y. Jin, M. Thon*, H. Pan*, E. Bhattarai, T. Thomas, R. Dean*

author keywords: plant pathogen
MeSH headings : Expressed Sequence Tags; Gene Expression Profiling; Gene Library; Genome, Fungal; Magnaporthe / genetics; Multigene Family; Oryza / microbiology
TL;DR: Manual annotation of the most abundant cDNAs with no known homologs allowed us to identify a family of metallothionein proteins present in M. grisea, Neurospora crassa, and Fusarium graminearum. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 journal article

Insight into Trichoderma reesei's genome content, organization and evolution revealed through BAC library characterization

FUNGAL GENETICS AND BIOLOGY, 41(12), 1077–1087.

By: S. Diener n, M. Chellappan n, T. Mitchell n, N. Dunn-Coleman, M. Ward & R. Dean n

author keywords: Trichoderma reesei; bacterial artificial chromosome (BAC); physical map; genome organization; secretion; biomass degradation; repetitive element
MeSH headings : Chromosome Mapping; Chromosomes, Artificial, Bacterial; Contig Mapping; DNA Fingerprinting; DNA Transposable Elements / genetics; DNA, Fungal / chemistry; DNA, Fungal / genetics; DNA, Fungal / isolation & purification; Fungal Proteins / genetics; Fungal Proteins / physiology; Genome, Fungal; Genomic Library; Molecular Sequence Data; Nucleic Acid Hybridization; Podospora / genetics; Polymorphism, Restriction Fragment Length; Sequence Analysis, DNA; Sequence Homology; Trichoderma / enzymology; Trichoderma / genetics
TL;DR: A genomic framework for Trichoderma reesei is created and several copies of an element with similarity to the Podospora anserina transposon, PAT are identified. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 journal article

Protocol for DNA extraction from watermelon leaves for SSR marker studies

Report (Cucurbit Genetics Cooperative), (27), 25.

By: G. Gusmini, T. Wehner, T. Joobeur, R. Dean & A. Levi

Source: NC State University Libraries
Added: August 6, 2018

2004 journal article

Robust-LongSAGE (RL-SAGE): A substantially improved LongSAGE method for gene discovery and transcriptome analysis

PLANT PHYSIOLOGY, 134(3), 890–897.

MeSH headings : Base Sequence; Cloning, Molecular; DNA, Plant / genetics; Gene Expression Profiling / methods; Genes, Plant; Genome, Plant; Genomics / methods; Plant Diseases / genetics; RNA, Messenger / genetics; RNA, Plant / genetics
TL;DR: A substantially improved LongSAGE method called Robust-LongSAGE, which has four major improvements when compared with the previously reported protocols, and can generate two to three libraries, each containing over 4.5 million tags, within a month. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 journal article

The fusarium wilt resistance locus Fom-2 of melon contains a single resistance gene with complex features

PLANT JOURNAL, 39(3), 283–297.

By: T. Joobeur n, J. King*, S. Nolin n, C. Thomas* & R. Dean n

author keywords: Fom-2; melon; complex R locus; fine mapping; BAC end sequence; R gene evolution
MeSH headings : Alleles; Amino Acid Sequence; Base Sequence; Chromosome Mapping; Cucumis melo / genetics; Cucumis melo / microbiology; DNA Primers / genetics; DNA, Plant / genetics; Evolution, Molecular; Fusarium / pathogenicity; Gene Conversion; Genes, Plant; Molecular Sequence Data; Plant Diseases / genetics; Plant Diseases / microbiology; Plant Proteins / chemistry; Plant Proteins / genetics; Polymorphism, Genetic; Recombination, Genetic; Selection, Genetic
TL;DR: The map-based cloning and characterization of the resistance gene Fom-2 that confers resistance to race 0 and 1 of this plant pathogen is reported, revealing new insights into R gene evolution. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

AFLP analysis of a worldwide collection of Didymella bryoniae

MYCOLOGICAL RESEARCH, 107, 297–304.

By: R. Kothera*, A. Keinath*, R. Dean n & M. Farnham*

MeSH headings : Ascomycota / classification; Ascomycota / genetics; Ascomycota / isolation & purification; Ascomycota / pathogenicity; Citrullus / microbiology; Cucumis / microbiology; DNA Primers; Genes, Fungal; Polymerase Chain Reaction; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length
TL;DR: Assessment of genetic variation within a worldwide collection of D. bryoniae isolates found that isolates from the northern USA grouped separately from isolates originating from the southern USA, and the degree of virulence was not strongly associated with AFLP groupings. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

An eight-cysteine-containing CFEM domain unique to a group of fungal membrane proteins

TRENDS IN BIOCHEMICAL SCIENCES, 28(3), 118–121.

By: R. Kulkarni n, H. Kelkar* & R. Dean n

MeSH headings : Amino Acid Sequence; Cysteine / chemistry; Cysteine / genetics; Fungal Proteins / chemistry; Fungal Proteins / genetics; Membrane Proteins / chemistry; Membrane Proteins / genetics; Molecular Sequence Data; Plant Proteins / chemistry; Plant Proteins / genetics; Plant Proteins / metabolism; Protein Structure, Tertiary; Sequence Alignment
TL;DR: Feature of CFEM suggest that it is a novel domain with characteristics distinct from known cysteine-rich domains, and some CFEM-containing proteins are proposed to have important roles in fungal pathogenesis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Characterization of the protein processing and secretion pathways in a comprehensive set of expressed sequence tags from Trichoderma reesei

FEMS MICROBIOLOGY LETTERS, 230(2), 275–282.

By: S. Diener n, N. Dunn-Coleman, P. Foreman, T. Houfek n, P. Teunissen, P. Solingen, L. Dankmeyer, T. Mitchell n, M. Ward, R. Dean n

author keywords: secretion; protein processing; Trichoderma reesei; expressed sequence tag
MeSH headings : Computational Biology; Expressed Sequence Tags; Fungal Proteins / genetics; Fungal Proteins / metabolism; Gene Library; Protein Processing, Post-Translational; Protein Transport; Sequence Analysis, DNA; Trichoderma / genetics
TL;DR: In an attempt to identify genes involved in protein folding and ornamentation steps in Trichoderma reesei, the 5' ends of 21888 cDNA clones were sequenced from which a unique set of over 5000 were also 3' sequenced. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Cloning, sequencing and functional analysis of Magnaporthe grisea MVP1 gene, a hex-1 homolog encoding a putative ‘woronin body’ protein

FEMS Microbiology Letters, 230(1), 85–90.

By: F. Asiegbu n, W. Choi n, J. Jeong n & R. Dean n

author keywords: appressorium; cDNA; gene disruption; Northern blotting; rice blast fungus
MeSH headings : Cloning, Molecular; Fungal Proteins / genetics; Fungal Proteins / metabolism; Gene Deletion; Gene Expression Regulation, Fungal; Magnaporthe / genetics; Magnaporthe / growth & development; Magnaporthe / metabolism; Magnaporthe / pathogenicity; Molecular Sequence Data; Oryza / microbiology; Plant Diseases / microbiology; Recombination, Genetic; Sequence Analysis, DNA
TL;DR: RNA gel blot analyses showed that MVP1 was highly expressed in germinating conidia and the mycelial stage compared to appressoria or non-germinated conidia, and double homologous recombination was used to replace MVP1 with the hyg(R) gene. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2003 journal article

Identification of proteins that interact with two regulators of appressorium development, adenylate cyclase and cAMP-dependent protein kinase A, in the rice blast fungus Magnaporthe grisea

Molecular Genetics and Genomics, 270(6), 497–508.

By: R. Kulkarni n & R. Dean n

MeSH headings : Adenylyl Cyclases / metabolism; Base Sequence; Cloning, Molecular; Cyclic AMP / physiology; Cyclic AMP-Dependent Protein Kinases / metabolism; DNA Primers; DNA, Complementary; Gene Library; Magnaporthe / enzymology; Magnaporthe / genetics; Magnaporthe / growth & development; Oryza / microbiology; Plant Diseases / microbiology; Plant Growth Regulators / pharmacology; Plant Proteins / metabolism; Restriction Mapping; Signal Transduction
TL;DR: To identify new components in the cAMP signal transduction pathway, the yeast two-hybrid system was used to screen MAC1 and CPKA against an appressorium cDNA library and phosphorylation motifs in these sequences suggest that they could be CPKA substrates. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2003 journal article

In-depth view of structure, activity, and evolution of rice chromosome 10

Science, 300(5625), 1566–1569.

Ralph Dean

Source: NC State University Libraries
Added: August 6, 2018

2003 journal article

Isolation of a novel antimicrobial peptide gene (Sp-AMP) homologue from Pinus sylvestris (Scots pine) following infection with the root rot fungus Heterobasidion annosum

FEMS MICROBIOLOGY LETTERS, 228(1), 27–31.

By: F. Asiegbu n, W. Choi n, G. Li*, J. Nahalkova* & R. Dean n

author keywords: subtraction hybridization; Heterobasidion annosum; expressed sequence tag; Pinus sylvestris
MeSH headings : Antimicrobial Cationic Peptides; Base Sequence; Basidiomycota; Molecular Sequence Data; Mycoses / microbiology; Pinus sylvestris / genetics; Pinus sylvestris / microbiology; Plant Diseases / genetics; Plant Diseases / microbiology; Plant Proteins / genetics; Plant Roots / microbiology; Plant Roots / physiology; Sequence Homology, Amino Acid
TL;DR: A new family of antimicrobial peptide homologues termed Sp-Amp has been discovered in Pinus sylvestris (Scots pine) and is the first report of such proteins to be characterized in a conifer species. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Large scale parallel analysis of gene expression during infection-related morphogenesis of Magnaporthe grisea

MOLECULAR PLANT PATHOLOGY, 4(5), 337–346.

By: Y. Takano*, W. Choi n, T. Mitchell n, T. Okuno* & R. Dean n

TL;DR: Gene expression in ungerminated conidia, during appressorium formation, and during mycelial growth was investigated with a novel highly sensitive dendrimer based detection system and results provide a powerful resource for furthering understanding of gene expression during infection-related morphogenesis and for the functional analysis of M. grisea genes involved in fungal infection. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

PI3K signaling is required for prostaglandin-induced mucosal recovery in ischemia-injured porcine ileum

American Journal of Physiology-Gastrointestinal and Liver Physiology, 284(1), G46–G56.

By: D. Little*, R. Dean, K. Young*, S. McKane n, L. Martin n, S. Jones*, A. Blikslager*

author keywords: phosphatidylinositol 3-kinase; tight junction; occludin; zonula occludens-1
MeSH headings : 16,16-Dimethylprostaglandin E2 / pharmacology; Animals; Anti-Ulcer Agents / pharmacology; Electric Impedance; Epoprostenol / analogs & derivatives; Epoprostenol / pharmacology; Female; Humans; Ileum / metabolism; Intestinal Mucosa / enzymology; Intestinal Mucosa / ultrastructure; Ischemia / metabolism; Male; Membrane Proteins / metabolism; Microscopy, Electron; Occludin; Osmotic Pressure; Phosphatidylinositol 3-Kinases / metabolism; Phosphoproteins / metabolism; Platelet Aggregation Inhibitors / pharmacology; Recovery of Function / physiology; Signal Transduction / drug effects; Signal Transduction / physiology; Swine; Tight Junctions / enzymology; Urea / pharmacology; Zonula Occludens-1 Protein
TL;DR: These experiments suggest that PGs stimulate recovery of paracellular resistance via a mechanism involving transepithelial osmotic gradients and PI3K-dependent restoration of tight junction protein distribution. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: August 6, 2018

2003 review

The rice blast pathosystem as a case study for the development of new tools and raw materials for genome analysis of fungal plant pathogens

[Review of ]. NEW PHYTOLOGIST, 159(1), 53–61.

By: T. Mitchell n, M. Thon n, J. Jeong n, D. Brown n, J. Deng n & R. Dean n

author keywords: Magnaporthe grisea; rice blast; genomic tools; BAC; physical map; synteny; whole genome comparison; fungi
TL;DR: This review will describe the recent development of tools for whole or partial genome analysis and multigenome comparisons and focuses on the rice blast pathosystem as a case study. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Transcriptional regulation of biomass-degrading enzymes in the filamentous fungus Trichoderma reesei

JOURNAL OF BIOLOGICAL CHEMISTRY, 278(34), 31988–31997.

By: P. Foreman, D. Brown n, L. Dankmeyer, R. Dean n, S. Diener n, N. Dunn-Coleman, F. Goedegebuur, T. Houfek n ...

MeSH headings : Biomass; DNA, Complementary; Enzymes / genetics; Enzymes / metabolism; Expressed Sequence Tags; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Fungal; Hydrolysis; Molecular Sequence Data; Transcription, Genetic; Trichoderma / enzymology
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2002 journal article

Cloning of disease-resistance homologues in end sequences of BAC clones linked to Fom-2, a gene conferring resistance to Fusarium wilt in melon (Cucumis melo L.)

GENOME, 45(3), 473–480.

By: Y. Wang*, W. Choi, C. Thomas & R. Dean*

author keywords: BAC end sequencing; Cucumis melo L.; Fusarium wilt; R gene
MeSH headings : Amino Acid Sequence; Chromosomes, Artificial, Bacterial; Contig Mapping; Cucumis melo / genetics; Cucumis melo / microbiology; Fusarium; Gene Library; Genes, Plant; Genetic Markers; Molecular Sequence Data; Plant Diseases / genetics; Sequence Alignment
TL;DR: Two cosegregating codominant markers (AM, AFLP marker; FM, Fusarium marker) were used to screen a melon bacterial artificial chromosome library and sequence information may be useful for identifying NBS-LRR class of R genes in other cucurbits. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2002 journal article

Development of PCR-ELISA for detection and differentiation of Didymella bryoniae from related Phoma species

PLANT DISEASE, 86(7), 710–716.

By: B. Somai*, A. Keinath* & R. Dean n

TL;DR: PCR-ELISA was a highly specific, yet simple, rapid and convenient assay for detection of D. bryoniae or Phoma and was used successfully on crude extracts of "blind" fungal samples. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2002 journal article

Internal transcribed spacer regions 1 and 2 and random amplified polymorphic DNA analysis of Didymella bryoniae and related Phoma species isolated from cucurbits

PHYTOPATHOLOGY, 92(9), 997–1004.

By: B. Somai, R. Dean*, M. Farnham, T. Zitter & A. Keinath

TL;DR: Didymella bryoniae is the causal agent of gummy stem blight, although other Phoma species are often isolated from cucurbit plants exhibiting symptoms of the disease, and RAPD fingerprints and pathogenicity indicate that they represent two different molecular and virulence subgroups. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2002 journal article

MagnaportheDB: a federated solution for integrating physical and genetic map data with BAC end derived sequences for the rice blast fungus Magnaporthe grisea

NUCLEIC ACIDS RESEARCH, 30(1), 121–124.

By: S. Martin n, B. Blackmon, R. Rajagopalan, T. Houfek, R. Sceeles, S. Denn, T. Mitchell, D. Brown, R. Wing, R. Dean

MeSH headings : Base Sequence; Chromosome Mapping; Chromosomes, Artificial, Bacterial; Chromosomes, Fungal; Database Management Systems; Databases, Genetic; Forecasting; Genetic Markers; Genome, Fungal; Genomic Library; Information Storage and Retrieval; Internet; Magnaporthe / genetics; Oryza / microbiology; Plant Diseases
TL;DR: A federated database for genome studies of Magnaporthe grisea, the causal agent of rice blast disease, is created by integrating end sequence data from BAC clones, genetic marker data and BAC contig assembly data. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2002 journal article

Two novel fungal virulence genes specifically expressed in appressoria of the rice blast fungus

PLANT CELL, 14(9), 2107–2119.

By: C. Xue*, G. Park*, W. Choi n, L. Zheng*, R. Dean n & . Xu*

MeSH headings : Amino Acid Sequence; Cytoplasm / genetics; Cytoplasm / metabolism; Fungal Proteins / genetics; Fungal Proteins / metabolism; Fungal Structures / genetics; Fungal Structures / growth & development; Fungal Structures / metabolism; Gene Expression Regulation, Developmental; Gene Expression Regulation, Fungal / genetics; Hordeum / microbiology; Magnaporthe / genetics; Magnaporthe / growth & development; Magnaporthe / pathogenicity; Mitogen-Activated Protein Kinases / genetics; Mitogen-Activated Protein Kinases / metabolism; Molecular Sequence Data; Mutation; Oryza / microbiology; Phenotype; Phylogeny; Sequence Homology, Amino Acid; Virulence / genetics
TL;DR: A subtraction library enriched for genes regulated by PMK1 found that deletion of both GAS1 and GAS2 did not have an additive effect on appressorial penetration and lesion formation, and may belong to a class of proteins specific for filamentous fungi and function as novel virulence factors in fungal pathogens. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2001 journal article

A genetic linkage map for watermelon based on randomly amplified polymorphic DNA markers

JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 126(6), 730–737.

By: A. Levi n, C. Thomas n, X. Zhang n, T. Joobeur n, R. Dean n, T. Wehner*, B. Carle n

author keywords: Citrullus lanatus; genetic mapping; SCAR; fusarium wilt; RAPD
TL;DR: A genetic linkage map was constructed for watermelon using a BC1 population derived from a cross between an inbred line and a wild accession, indicating that the watermelon genome may contain large chromosomal regions that are deficient in recombination events. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2001 journal article

Global analysis of protein activities using proteome chips

SCIENCE, 293(5537), 2101–2105.

By: H. Zhu*, M. Bilgin*, R. Bangham*, D. Hall*, A. Casamayor*, P. Bertone*, N. Lan*, R. Jansen* ...

MeSH headings : Amino Acid Motifs; Amino Acid Sequence; Calmodulin / metabolism; Calmodulin-Binding Proteins / metabolism; Cell Membrane / metabolism; Cloning, Molecular; Fungal Proteins / chemistry; Fungal Proteins / genetics; Fungal Proteins / metabolism; Glucose / metabolism; Liposomes / metabolism; Membrane Proteins / metabolism; Molecular Sequence Data; Open Reading Frames; Peptide Library; Phosphatidylcholines / metabolism; Phosphatidylinositols / metabolism; Phospholipids / metabolism; Protein Binding; Proteome; Recombinant Fusion Proteins / metabolism; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Signal Transduction; Streptavidin / metabolism
TL;DR: Microarrays of an entire eukaryotic proteome can be prepared and screened for diverse biochemical activities and can be used to screen protein-drug interactions and to detect posttranslational modifications. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2001 journal article

Melon bacterial artificial chromosome (BAC) library construction using improved methods and identification of clones linked to the locus conferring resistance to melon Fusarium wilt (Fom-2)

GENOME, 44(2), 154–162.

By: M. Luo, Y. Wang, D. Frisch, T. Joobeur, R. Wing & R. Dean*

author keywords: bacterial artificial chromosome (BAC) library; Fusarium wilt; melon; pCUGIBAC1; resistant gene
TL;DR: Two bacterial artificial chromosome libraries were constructed for the multidisease-resistant line of melon MR-1, using improved methods and screened with two genetic markers FM and AM that co-segregate with Fom-2, a gene conferring resistance to races 0 and 1 of Fusarium wilt. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2000 journal article

Genetic mapping of a fusarium wilt resistance gene (Fom-2) in melon (Cucumis melo L.)

MOLECULAR BREEDING, 6(4), 379–389.

By: Y. Wang*, C. Thomas* & R. Dean n

author keywords: AFLP; co-dominant markers; Cucumis melo; fusarium wilt; marker-assisted selection
TL;DR: A survey of 45 melon genotypes from diverse geographic origins with the co-dominant markers demonstrated a high correlation between fragment size and the resistance phenotype, and these markers may therefore be useful in marker-assisted breeding programs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2000 journal article

Site-directed mutagenesis of the magB gene affects growth and development in Magnaporthe grisea

MOLECULAR PLANT-MICROBE INTERACTIONS, 13(11), 1214–1227.

By: E. Fang* & R. Dean n

author keywords: rice blast; signal transduction
MeSH headings : Amino Acid Sequence; Cell Differentiation / genetics; Crosses, Genetic; Cyclic AMP / analysis; Fungal Proteins / genetics; GTP-Binding Protein alpha Subunits; Genes, Fungal; Heterotrimeric GTP-Binding Proteins / genetics; Magnaporthe / cytology; Magnaporthe / genetics; Magnaporthe / growth & development; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Oryza / microbiology; Phenotype; Plant Diseases / genetics; Plasmids; Transformation, Genetic
TL;DR: Comparative analyses of the phenotypical effects of different magB mutations are consistent with the involvement of the Gbetagamma subunit in the signaling pathways regulating cellular development in M. grisea. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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