Ryan Kilgore

Chemical & Biomolecular G&T

Works (12)

Updated: January 21st, 2026 14:13

2025 journal article

Integrating Affinity Chromatography in the Platform Process for Adenovirus Purification

Biotechnology and Bioengineering, 122(7), 1780–1792.

By: Y. Wu n, E. Barbieri n, W. Smith n, A. Minzoni n, R. Kilgore n, W. Chu n, M. Daniele n , S. Menegatti n 

Contributors: Y. Wu n, E. Barbieri n, W. Smith n, A. Minzoni n, R. Kilgore n, W. Chu n, M. Daniele n , S. Menegatti n 

author keywords: adenovirus; affinity chromatography; biomanufacturing; bioseparations; gene therapy
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; Viral gastroenteritis research and epidemiology
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: May 6, 2025

2025 article

Integrating affinity chromatography in the platform process for Adenovirus purification

Wu, Y., Barbieri, E., Smith, W. K., Minzoni, A., Kilgore, R. E., Chu, W., … Menegatti, S. (2025, April 5). (Vol. 4). Vol. 4.

By: Y. Wu n, E. Barbieri*, W. Smith n, A. Minzoni n, R. Kilgore n, W. Chu n, M. Daniele n , S. Menegatti n 

Contributors: Y. Wu n, E. Barbieri*, W. Smith n, A. Minzoni n, R. Kilgore n, W. Chu n, M. Daniele n , S. Menegatti n 

topics (OpenAlex): Viral Infectious Diseases and Gene Expression in Insects; Protein purification and stability; Virus-based gene therapy research
Sources: NC State University Libraries, NC State University Libraries, ORCID
Added: January 18, 2026

2025 article

Purification of CRISPR Cas12a from E. coli cell lysates using peptide affinity ligands

Wu, Y., Kilgore, R., Hetzler, Z., Chu, W., Moore, B., Barbieri, E., … Menegatti, S. (2025, May 17). Journal of Chromatography A, Vol. 1755.

By: Y. Wu n, R. Kilgore n, Z. Hetzler n, W. Chu n, B. Moore n, E. Barbieri n, Q. Wei n , S. Menegatti n 

author keywords: CRISPR Cas12a; Gene editing; Peptide ligands; Affinity chromatography; Protein purification
MeSH headings : Escherichia coli / chemistry; Escherichia coli / genetics; Escherichia coli / enzymology; Chromatography, Affinity / methods; Ligands; CRISPR-Associated Proteins / isolation & purification; CRISPR-Associated Proteins / metabolism; CRISPR-Associated Proteins / chemistry; Peptides / chemistry; Peptides / metabolism; CRISPR-Cas Systems; Bacterial Proteins / isolation & purification; Bacterial Proteins / metabolism; Bacterial Proteins / chemistry; Endodeoxyribonucleases / isolation & purification; Endodeoxyribonucleases / metabolism; Endodeoxyribonucleases / chemistry; Acidaminococcus / enzymology
topics (OpenAlex): CRISPR and Genetic Engineering; RNA and protein synthesis mechanisms; Advanced biosensing and bioanalysis techniques
Sources: Web Of Science, NC State University Libraries
Added: June 9, 2025

2024 journal article

Peptide ligands for the affinity purification of adenovirus from HEK293 and vero cell lysates

Journal of Chromatography A, 1736, 465396.

By: Y. Wu n, E. Barbieri n, R. Kilgore n, B. Moore n, W. Chu n, G. Mollica n, M. Daniele n , S. Menegatti n 

Contributors: Y. Wu n, E. Barbieri n, R. Kilgore n, B. Moore n, W. Chu n, G. Mollica n, M. Daniele n , S. Menegatti n 

author keywords: Affinity chromatography; Adenovirus; Viral vectors; Gene therapy; Peptide ligands
MeSH headings : Humans; Chromatography, Affinity / methods; Chlorocebus aethiops; Animals; Ligands; Vero Cells; HEK293 Cells; Peptides / chemistry; Peptides / isolation & purification; Peptides / metabolism; Adenoviridae / chemistry; Adenoviridae / isolation & purification; Capsid Proteins / isolation & purification; Capsid Proteins / metabolism; Capsid Proteins / chemistry
topics (OpenAlex): Monoclonal and Polyclonal Antibodies Research; Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: October 14, 2024

2024 journal article

Peptide ligands for the universal purification of exosomes by affinity chromatography

Biotechnology and Bioengineering, 121(11), 3484–3501.

By: R. Kilgore n, B. Moore n, S. Sripada n, W. Chu n, S. Shastry n, E. Barbieri n, S. Hu n , W. Tian * ...

Contributors: R. Kilgore n, B. Moore n, S. Sripada n, W. Chu n, S. Shastry n, E. Barbieri n, S. Hu n , . al.

author keywords: affinity chromatography; bioseparations; exosomes; gene delivery; peptide ligands
MeSH headings : Exosomes / chemistry; Exosomes / metabolism; Chromatography, Affinity / methods; Ligands; Humans; Peptides / chemistry; Peptides / metabolism; Peptides / isolation & purification
topics (OpenAlex): Extracellular vesicles in disease; RNA Interference and Gene Delivery; Inhalation and Respiratory Drug Delivery; Lipid Membrane Structure and Behavior
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 14, 2024

2023 journal article

Peptide ligands for the affinity purification of adeno‐associated viruses from HEK 293 cell lysates

Biotechnology and Bioengineering, 120(8), 2283–2300.

By: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback‐Clarke n, W. Smith n, B. Moore n, R. Kilgore n ...

Contributors: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback-Clarke n, W. Smith n, B. Moore n, . al.

author keywords: adeno-associated virus; affinity chromatography; HEK 293 lysate; peptide ligands; virus purification
MeSH headings : Humans; Dependovirus / genetics; HEK293 Cells; Ligands; Peptides / genetics; Peptides / metabolism; Amino Acid Sequence; Genetic Vectors
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; RNA Interference and Gene Delivery
TL;DR: Peptide ligands that selectively capture AAVs and release them under mild conditions are introduced, affording high recovery, high recovery of host cell proteins (HCPs), and high transduction activity of the purified viruses. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 7, 2023

2023 journal article

Peptide ligands targeting the vesicular stomatitis virus G (VSV‐G) protein for the affinity purification of lentivirus particles

Biotechnology and Bioengineering, 121(2), 618–639.

By: E. Barbieri n, G. Mollica n, B. Moore n, S. Sripada n, S. Shastry n, R. Kilgore n, C. Loudermilk n, Z. Whitacre n ...

author keywords: affinity chromatography; cell therapy; lentivirus; peptide ligands; vesicular stomatitis virus G protein
MeSH headings : Animals; Humans; Lentivirus / genetics; Lentivirus / metabolism; Vesicular Stomatitis; HEK293 Cells; Peptides / metabolism; Vesiculovirus / genetics; Genetic Vectors
topics (OpenAlex): Virus-based gene therapy research; Virology and Viral Diseases; Bacteriophages and microbial interactions
TL;DR: This study introduces peptides targeting the enveloped protein Vesicular stomatitis virus G (VSV‐G) to serve as affinity ligands for the chromatographic purification of LV particles. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: January 29, 2024

2023 article

The downstream bioprocess toolbox for therapeutic viral vectors

Kilgore, R., Minzoni, A., Shastry, S., Smith, W., Barbieri, E., Wu, Y., … Menegatti, S. (2023, September 9). Journal of Chromatography A, Vol. 1709.

By: R. Kilgore n, A. Minzoni n, S. Shastry n, W. Smith n, E. Barbieri n, Y. Wu n, J. LeBarre n, W. Chu n, J. O'Brien n, S. Menegatti n 

author keywords: Viral vectors; Gene therapy; Affinity ligands; Chromatography; Adsorbents; AAV
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; Bacteriophages and microbial interactions
TL;DR: This study presents a comprehensive overview of the downstream bioprocess toolbox for viral vectors established in the last decade, and discusses present efforts and future directions contributing to the success of this promising class of biological medicines. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: October 30, 2023

2022 article

De novo discovery of peptide-based affinity ligands for the fab fragment of human immunoglobulin G

Xiao, X., Kilgore, R., Sarma, S., Chu, W., Menegatti, S., & Hall, C. K. (2022, March 6). Journal of Chromatography A, Vol. 4.

By: X. Xiao n, R. Kilgore n, S. Sarma n, W. Chu n, S. Menegatti*  & C. Hall* 

author keywords: Affinity ligands; Ligand design; Biorecognition; Protein chromatography; Fragment antigen binding
MeSH headings : Humans; Immunoglobulin Fab Fragments / chemistry; Immunoglobulin G; Ligands; Peptides; Tissue Distribution
topics (OpenAlex): Monoclonal and Polyclonal Antibodies Research; Protein purification and stability; Glycosylation and Glycoproteins Research
TL;DR: An integrated computational and experimental scheme is described to de novo design 9-mer peptides that bind to Fab fragments that lay the groundwork for future development of these ligands towards biomanufacturing translation. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: May 2, 2022

2022 article

Development of peptide affinity ligands for the purification of polyclonal and monoclonal Fabs from recombinant fluids

Kilgore, R., Chu, W., Bhandari, D., Fischler, D., Carbonell, R. G., Crapanzano, M., & Menegatti, S. (2022, December 5). Journal of Chromatography A, Vol. 1687.

By: R. Kilgore n, W. Chu n, D. Bhandari, D. Fischler, R. Carbonell n, M. Crapanzano, S. Menegatti n 

author keywords: Peptide ligands; Affinity chromatography; Fragment antigen binding; Monoclonal antibodies; Cell culture harvests
MeSH headings : Cricetinae; Animals; Humans; Cricetulus; Ligands; CHO Cells; Molecular Docking Simulation; Protein Binding; Peptides / chemistry; Antibodies, Monoclonal; Chromatography, Affinity
topics (OpenAlex): Monoclonal and Polyclonal Antibodies Research; Protein purification and stability; Viral Infectious Diseases and Gene Expression in Insects
TL;DR: The de novo discovery of peptide ligands that target different regions of human Fab and enable product release under mild conditions are discovered and afforded values of yield and purity comparable to those provided by Protein L resins. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: January 17, 2023

2022 article

Development of peptide ligands for the purification of α-1 antitrypsin from cell culture fluids

Chu, W., Prodromou, R., Moore, B., Elhanafi, D., Kilgore, R., Shastry, S., & Menegatti, S. (2022, July 22). Journal of Chromatography A, Vol. 1679.

By: W. Chu n, R. Prodromou n, B. Moore n, D. Elhanafi n, R. Kilgore n, S. Shastry n, S. Menegatti n 

author keywords: a-1 antitrypsin; Peptide ligands; Affinity chromatography; Protein purification; CHO cell culture harvest
MeSH headings : Animals; CHO Cells; Cell Culture Techniques; Chromatography, Affinity; Cricetinae; Cricetulus; Ligands; Molecular Docking Simulation; Peptides; alpha 1-Antitrypsin
topics (OpenAlex): Protein Hydrolysis and Bioactive Peptides; Silk-based biomaterials and applications; Chemical Synthesis and Analysis
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Sources: Web Of Science, NC State University Libraries
Added: October 11, 2022

2020 article

Peptides and pseudopeptide ligands: a powerful toolbox for the affinity purification of current and next-generation biotherapeutics

Chu, W., Prodromou, R., Day, K. N., Schneible, J. D., Bacon, K. B., Bowen, J. D., … Menegatti, S. (2020, October 23). Journal of Chromatography A, Vol. 1635.

By: W. Chu n, R. Prodromou n, K. Day n, J. Schneible n, K. Bacon n, J. Bowen n, R. Kilgore n, C. Catella n ...

author keywords: Affinity ligands; peptides; peptoids; pseudopeptides; biotherapeutics
MeSH headings : Antibodies / isolation & purification; Biological Products / isolation & purification; Chemistry, Pharmaceutical / methods; Chromatography, Affinity; Family Characteristics; Humans; Ligands; Peptides / isolation & purification; Peptoids / chemistry; Proteins / isolation & purification
topics (OpenAlex): Monoclonal and Polyclonal Antibodies Research; Chemical Synthesis and Analysis; Click Chemistry and Applications
TL;DR: This work presents a comprehensive and systematic review of the literature on peptide-based ligands and their use in the affinity purification of established and upcoming biological drugs. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: January 25, 2021

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