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Works (47)
Updated: September 11th, 2024 05:01
2024 journal article
Address correspondence to Raquel G. Hernandez, MD, MPH, (e-mail: Raquel.hernandez@jhmi.edu).
ACADEMIC PEDIATRICS, 24(7), 1076–1085.
author keywords: anxiety; children; COVID-19; maternal stress; pandemic; psychosocial stress
Sources: Web Of Science, NC State University Libraries
Added: September 9, 2024
2024 journal article
Maternal childhood adversity and infant epigenetic aging: Moderation by restless sleep during pregnancy
DEVELOPMENTAL PSYCHOBIOLOGY, 66(2).
author keywords: adverse childhood experience; epigenomics; pregnancy; sleep
TL;DR:
Preliminary evidence is provided that maternal childhood adversity and sleep may influence the infant epigenome and there was evidence of an interaction between maternal ACEs and restless sleep in predicting infant epigenetic age acceleration using the EPIC gestational age clock.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: February 12, 2024
2020 journal article
The impact of Zika virus infection on human semen: a case study
FUTURE VIROLOGY, 15(1), 5–12.
author keywords: assisted reproduction; Flavivirus persistence; immunofluorescence; longitudinal study; semen; semen donor; sexually transmitted virus; super-resolution microscopy; virus clearance; Zika virus
TL;DR:
A significant decrease in sperm concentration and in the percentage of progressive motility of sperm was observed, but the complete clearance of ZIKV from semen was not achieved.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: September 7, 2020
2019 journal article
Purification and Proteomic Analysis of Alphavirus Particles from Sindbis Virus Grown in Mammalian and Insect Cells
BIO-PROTOCOL, 9(10).
author keywords: Sindbis virus; Mass spectrometry; Virus proteome; Virus purification; Alphavirus; Proteomics
TL;DR:
To evaluate the efficacy of mass spec to determine the proteome of medically important viruses, Sindbis virus (SINV), the prototypical alphavirus was chosen for evaluation and it was found that in addition to the host proteins, SINV non-structural protein 2 (nsP2) was detected within virions grown in all host cells examined.
(via Semantic Scholar)
open access via doi.org (publisher)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: June 17, 2019
2018 journal article
Comparative Characterization of the Sindbis Virus Proteome from Mammalian and Invertebrate Hosts Identifies nsP2 as a Component of the Virion and Sorting Nexin 5 as a Significant Host Factor for Alphavirus Replication
JOURNAL OF VIROLOGY, 92(14).
author keywords: alphavirus; mass spectrometry; Sindbis virus; viral proteomics
MeSH headings : Alphavirus Infections / metabolism; Alphavirus Infections / virology; Amino Acid Sequence; Animals; Cricetinae; Culicidae / metabolism; Culicidae / virology; Cysteine Endopeptidases / metabolism; HEK293 Cells; Humans; Proteome / metabolism; Sequence Homology; Sindbis Virus / physiology; Sorting Nexins / metabolism; Virion; Virus Replication
TL;DR:
Mass spectrometry-based analysis of the Sindbis virus proteome can be effective in identifying host genes involved in virus replication and novel functions for virus proteins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2018 journal article
Role of the vacuolar ATPase in the Alphavirus replication cycle
Heliyon, 4(7), e00701.
author keywords: Virology; Cell biology
TL;DR:
Results show that a functional V-ATPase is required for efficient virus RNA synthesis and virus maturation in Alphavirus infection.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Crossref
Added: June 6, 2020
2016 journal article
Single-Site Glycoprotein Mutants Inhibit a Late Event in Sindbis Virus Assembly
JOURNAL OF VIROLOGY, 90(18), 8372–8380.
MeSH headings : Animals; Cells, Cultured; Cricetinae; Membrane Glycoproteins / genetics; Membrane Glycoproteins / metabolism; Microscopy, Electron; Mutant Proteins / genetics; Mutant Proteins / metabolism; Mutation, Missense; Point Mutation; Sequence Deletion; Sindbis Virus / genetics; Sindbis Virus / physiology; Sindbis Virus / ultrastructure; Suppression, Genetic; Temperature; Viral Envelope Proteins / genetics; Viral Envelope Proteins / metabolism; Viral Load; Virion / ultrastructure; Virus Assembly; Virus Cultivation; Virus Release
TL;DR:
It is concluded that H230 is essential for the assembly of complete infectious Sindbis virus virions and that the presence of an amino acid at E2 position 209 is required for complete budding of Sind Bis virus particles although several different amino acids can be at this location without affecting the titer.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
The role of the vacuolar ATPase in alphavirus replication.
Molecular Biology of the Cell, 27.
Source: NC State University Libraries
Added: August 6, 2018
2015 review
Alphavirus Entry into Host Cells
[Review of ]. Molecular basis of viral infection, 129, 33–62.
Source: NC State University Libraries
Added: August 6, 2018
2014 journal article
Live Attenuated Tetravalent Dengue Virus Host Range Vaccine Is Immunogenic in African Green Monkeys following a Single Vaccination
JOURNAL OF VIROLOGY, 88(12), 6729–6742.
MeSH headings : Animals; Antibodies, Viral / immunology; Chlorocebus aethiops; Dengue / immunology; Dengue / prevention & control; Dengue / virology; Dengue Vaccines / administration & dosage; Dengue Vaccines / genetics; Dengue Vaccines / immunology; Dengue Virus / classification; Dengue Virus / genetics; Dengue Virus / immunology; Dengue Virus / physiology; Host Specificity; Humans; Species Specificity; Vaccination; Vaccines, Attenuated / administration & dosage; Vaccines, Attenuated / genetics; Vaccines, Attenuated / immunology; Viral Envelope Proteins / administration & dosage; Viral Envelope Proteins / genetics; Viral Envelope Proteins / immunology
TL;DR:
A live attenuated viral (LAV) vaccine capable of eliciting a strong immune response in African green monkeys (AGMs) in a single dose is generated, and this study suggests that viremia and antibody response in AGMs may be similar to those seen in DENV infection in humans.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Chikungunya Virus Host Range E2 Transmembrane Deletion Mutants Induce Protective Immunity against Challenge in C57BL/6J Mice
JOURNAL OF VIROLOGY, 87(12), 6748–6757.
MeSH headings : Alphavirus Infections / immunology; Alphavirus Infections / prevention & control; Alphavirus Infections / virology; Animals; Antibodies, Neutralizing / blood; Antibodies, Viral / blood; Cell Line; Chikungunya Fever; Chikungunya virus / genetics; Chikungunya virus / immunology; Chikungunya virus / pathogenicity; Chikungunya virus / physiology; Host Specificity; Humans; Mice; Mice, Inbred C57BL; Mutation; Sequence Deletion; Vaccines, Attenuated / genetics; Vaccines, Attenuated / immunology; Viral Vaccines / genetics; Viral Vaccines / immunology; Virus Replication
TL;DR:
This study identified a ChikV HR mutant that grows to high levels in insect cells but was restricted in the ability to assemble virus in mammalian cells in vitro.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Flavivirus infection from mosquitoes in vitro reveals cell entry at the plasma membrane
VIROLOGY, 435(2), 406–414.
author keywords: Flavivirus; Virus entry; Mosquitoes; Electron microscopy
MeSH headings : Animals; Cell Membrane / ultrastructure; Cell Membrane / virology; Chlorocebus aethiops; Culex / virology; Culicidae / virology; Dengue Virus / pathogenicity; Dengue Virus / ultrastructure; Flavivirus / pathogenicity; Microscopy, Electron; Vero Cells / virology; Virus Internalization; West Nile virus / pathogenicity; West Nile virus / ultrastructure
TL;DR:
The results show that Dengue and West Nile viruses may infect cells by a mechanism that involves direct penetration of the host cell plasma membrane as proposed for alphaviruses.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Infection of cells by alphaviruses
Viral Molecular Machines, 726, 181–199.
Source: NC State University Libraries
Added: August 6, 2018
2012 journal article
Testing of Novel Dengue Virus 2 Vaccines in African Green Monkeys: Safety, Immunogenicity, and Efficacy
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 87(4), 743–753.
MeSH headings : Animals; Antibodies, Neutralizing / blood; Antibodies, Viral / blood; Cells, Cultured; Chlorocebus aethiops; Dengue / immunology; Dengue / prevention & control; Dengue / virology; Dengue Vaccines / adverse effects; Dengue Vaccines / genetics; Dengue Vaccines / immunology; Dengue Vaccines / therapeutic use; Dengue Virus / genetics; Dengue Virus / immunology; Immunization; Neutralization Tests; Viral Envelope Proteins / genetics; Viral Envelope Proteins / immunology; Viral Plaque Assay
TL;DR:
Three novel host-range vaccines containing deletions in the transmembrane domain of dengue virus serotype 2 (DV2) E glycoprotein were evaluated in African green monkeys, and all three vaccine strains decreased the length of viremia by at least two days.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
Conformational Changes in Sindbis Virus Induced by Decreased pH Are Revealed by Small-Angle Neutron Scattering
JOURNAL OF VIROLOGY, 86(4), 1982–1987.
MeSH headings : Hydrogen-Ion Concentration; RNA, Viral / chemistry; RNA, Viral / genetics; RNA, Viral / metabolism; Scattering, Small Angle; Sindbis Virus / chemistry; Sindbis Virus / genetics; Sindbis Virus / metabolism; Viral Proteins / chemistry; Viral Proteins / genetics; Virion / chemistry; Virion / genetics; Virion / metabolism
TL;DR:
Small-angle neutron scattering with contrast variation is employed to probe how the structure of a mammalian-grown Sindbis virus responds to moderately acidic pH and improves the understanding of the pH-driven alteration of Sind Bis virus structure.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2011 journal article
Structural mutants of dengue virus 2 transmembrane domains exhibit host-range phenotype
VIROLOGY JOURNAL, 8.
MeSH headings : Aedes; Amino Acid Sequence; Animals; Antibodies, Neutralizing / blood; Antibodies, Viral / blood; Cell Line; Dengue Virus / genetics; Dengue Virus / immunology; Dengue Virus / pathogenicity; Dengue Virus / physiology; Host Specificity; Mice; Mice, Inbred BALB C; Models, Molecular; Molecular Sequence Data; Mutant Proteins / genetics; Mutant Proteins / immunology; Mutant Proteins / metabolism; Sequence Deletion; Viral Envelope Proteins / genetics; Viral Envelope Proteins / immunology; Viral Envelope Proteins / metabolism
TL;DR:
Novel host-range mutants of DV2 were created that have preferential growth in insect cells and impaired infectivity in mammalian cells and may be applied to many other insect-borne viral diseases for which no current effective therapies exist.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Growth and Maintenance of Baby Hamster Kidney (BHK) Cells
Current Protocols in Microbiology, 17(1), A.4H.1-A.4H.7.
MeSH headings : Animals; Cell Culture Techniques; Cell Line; Cricetinae; Culture Media / chemistry; Fibroblasts; Kidney / cytology; Mesocricetus; Sindbis Virus / growth & development; Virus Cultivation / methods
TL;DR:
The BHK21 cell line was established in 1961 from the kidneys of 5 Syrian hamsters from litter number 21 and has been a laboratory standard for the growth of countless viruses and the study of many biological processes.
(via Semantic Scholar)
Source: Crossref
Added: August 14, 2021
2010 journal article
Growth and Maintenance of Chick Embryo Fibroblasts (CEF)
Current Protocols in Microbiology, 17(1), A.4I.1-A.4I.8.
MeSH headings : Animals; Cell Culture Techniques; Cells, Cultured; Chick Embryo; Culture Media / chemistry; Fibroblasts
TL;DR:
Primary cultures of chick embryo fibroblasts are widely used for the cultivation of viruses and Protocols for the growth and maintenance of CEF cells in the laboratory are provided.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Source: Crossref
Added: August 16, 2021
2010 journal article
Growth and Maintenance of Mosquito Cell Lines
Current Protocols in Microbiology, 17(1), A.4J.1-A.4J.8.
MeSH headings : Animals; Cell Culture Techniques / methods; Cell Line; Culicidae; Culture Media / chemistry
TL;DR:
Mosquito cells (Aedes albopictus) are among the most common insect cells emerging as new sources of cell cultures to use in basic research and in the pharmaceutical industry.
(via Semantic Scholar)
Source: Crossref
Added: October 9, 2021
2010 journal article
Insect response to alphavirus infection-Establishment of alphavirus persistence in insect cells involves inhibition of viral polyprotein cleavage
VIRUS RESEARCH, 150(1-2), 73–84.
author keywords: Arbovirus; Sindbis; Insect cells; Microarray; Persistent infection
MeSH headings : Alphavirus / pathogenicity; Alphavirus / physiology; Animals; Cell Line; Drosophila; Polyproteins / metabolism; Viral Proteins / metabolism; Virus Replication
TL;DR:
It is proposed that inefficient cleavage of the polyprotein may lead to reduced levels of virus seen as persistence is established, and the up-regulation of a wide-spectrum protease inhibitor, Thiol-ester containing Protein (TEP) II is confirmed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Role of the Vacuolar-ATPase in Sindbis Virus Infection
JOURNAL OF VIROLOGY, 85(3), 1257–1266.
MeSH headings : Animals; Cells, Cultured; Cricetinae; Enzyme Inhibitors / metabolism; Host-Pathogen Interactions; Macrolides / metabolism; Sindbis Virus / physiology; Vacuolar Proton-Translocating ATPases / metabolism; Virus Internalization; Virus Replication
TL;DR:
The results suggest that in BHK cells in the presence of bafilomycin A1, virus RNA enters the cell and is translated, but replication and proper folding of the product proteins requires the function of the V-ATPase.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
The Structure of Sindbis Virus Produced from Vertebrate and Invertebrate Hosts as Determined by Small-Angle Neutron Scattering
JOURNAL OF VIROLOGY, 84(10), 5270–5276.
MeSH headings : Animals; Cell Line; Cricetinae; Culicidae; Scattering, Small Angle; Sindbis Virus / chemistry; Sindbis Virus / growth & development; Virion / chemistry; Virion / isolation & purification
TL;DR:
The first study of the structural differences between Sindbis viruses grown in mammalian and insect cells are reported, which were determined by small-angle neutron scattering (SANS), a nondestructive technique that did not decrease the infectivity of the Sindbis virus particles studied.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2009 journal article
A high capacity Alphavirus heterologous gene delivery system
VIROLOGY, 390(2), 368–373.
author keywords: Alphavirus; Gene delivery; Morphological variants
MeSH headings : Animals; Cell Line; Cricetinae; Culicidae; Gene Transfer Techniques; Genetic Vectors; Microscopy, Electron, Transmission; Mutation, Missense; Sindbis Virus / genetics; Viral Proteins / genetics; Virion / ultrastructure
TL;DR:
The first generation prototype larger vehicle, described here, carries a approximately 18 kb cDNA insert, however it is conceivable that RNA as large as 32 kb could be transcribed and packaged, and the resulting cargo containing virus particles are infectious.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
Differential Incorporation of Cholesterol by Sindbis Virus Grown in Mammalian or Insect Cells
JOURNAL OF VIROLOGY, 83(18), 9113–9121.
MeSH headings : Alphavirus Infections / etiology; Animals; Cell Line; Cell Membrane / chemistry; Cholesterol / analysis; Cholesterol / deficiency; Cholesterol / metabolism; Insecta; Lipids / analysis; Mammals; Mass Spectrometry; Sindbis Virus / metabolism; Sindbis Virus / pathogenicity
TL;DR:
The cholesterol dependence reported for infectivity and assembly of Sindbis virus is a reflection of differences in the insect cell lines used and the methods of delipidation, and the amount of cholesterol in the host cell membrane in and of itself has no effect on the process of virus assembly or on the ability of virus to infect cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
12. Responsible Consumption and Production
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
Helical virus particles formed from morphological subunits of a membrane containing icosahedral virus
VIROLOGY, 385(2), 285–293.
author keywords: Sindbis virus; Virus assembly; Protein interactions; Morphological variants; Helix symmetry; Icosahedral symmetry
MeSH headings : Animals; Cricetinae; Furin / metabolism; Membrane Glycoproteins / genetics; Membrane Glycoproteins / physiology; Microscopy, Electron, Scanning; Mutation; Sindbis Virus / chemistry; Sindbis Virus / physiology; Sindbis Virus / ultrastructure; Viral Proteins / metabolism; Virion / chemistry; Virus Assembly
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 review
Sindbis virus as a model for studies of conformational changes in a metastable virus and the role of conformational changes in in vitro antibody neutralisation
[Review of ]. REVIEWS IN MEDICAL VIROLOGY, 19(5), 257–272.
MeSH headings : Alphavirus Infections / immunology; Animals; Antibodies, Viral / chemistry; Antibodies, Viral / immunology; Neutralization Tests; Protein Conformation; Sindbis Virus / immunology; Viral Proteins / chemistry; Viral Proteins / immunology; Virus Assembly
TL;DR:
A model is presented which could extend the mechanism of antibody induced conformational neutralisation to any metastable virus structure, and this review will focus on the structural aspects of in vitro antibody neutralisation of a metastable alphavirus, Sindbis virus.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2008 journal article
Construction and characterization of mutant Dengue2 virus vaccine candidates displaying a host-range phenotype
Vaccine.
Source: NC State University Libraries
Added: August 6, 2018
2008 journal article
Sindbis virus conformational changes induced by a neutralizing anti-E1 monoclonal antibody
JOURNAL OF VIROLOGY, 82(12), 5750–5760.
MeSH headings : Animals; Antibodies, Monoclonal / pharmacology; Cricetinae; Epitopes; Hydrogen-Ion Concentration; Immunoglobulin Fab Fragments / immunology; Kidney / cytology; Membrane Glycoproteins / immunology; Neutralization Tests; Peptide Mapping; Protein Binding; Protein Conformation; Sindbis Virus / chemistry; Sindbis Virus / physiology; Sindbis Virus / ultrastructure; Viral Envelope Proteins / immunology; Virion / physiology; Virion / ultrastructure
TL;DR:
A model is proposed which postulates that the induction of conformational changes in the infectious state of a metastable enveloped virus may be a general mechanism of antibody inactivation of virus infectivity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2007 journal article
Infection of cells by Sindbis virus at low temperature
Virology, 362(2), 461–467.
MeSH headings : Animals; Cell Fusion; Cell Line; Cricetinae; Endocytosis; Microscopy, Immunoelectron; Neutral Red / metabolism; Sindbis Virus / physiology; Temperature; Time Factors; Viral Plaque Assay; Virus Internalization
TL;DR:
The ability of Sind Bis virus to infect Baby Hamster Kidney cells at temperatures which block endocytosis is examined and it is found that under these conditions Sindbis virus infects cells in a temperature- and time-dependent fashion.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018
2007 journal article
Location and role of free cysteinyl residues in the Sindbis virus E1 and E2 glycoproteins
JOURNAL OF VIROLOGY, 81(12), 6231–6240.
MeSH headings : Amino Acid Sequence; Animals; Capsid / chemistry; Cell Line; Chromatography, Liquid; Cricetinae; Cysteine / chemistry; Disulfides / chemistry; Iodoacetamide / pharmacology; Mass Spectrometry; Membrane Glycoproteins / chemistry; Molecular Sequence Data; Mutation; Protein Conformation; Viral Envelope Proteins / chemistry
TL;DR:
Native virus particles alkylated with iodoacetamide demonstrated a 4-log decrease in viral infectivity, which suggests that the modification of free cysteinyl residues results in the loss of infectivity by destabilizing the virus particle or that a rearrangement of disulfide bonds, which is required forinfectivity, is blocked by the modification.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
A colorimetric assay for viral agents that produce cytopathic effects
Journal of Virological Methods, 135(1), 56–65.
Source: NC State University Libraries
Added: August 6, 2018
2006 journal article
A colorimetric assay for viral agents that produce cytopathic effects
Journal of Virological Methods, 135(1), 56–65.
author keywords: MTT; TCID50; plaque assay; parvovirus; Sindbis virus
MeSH headings : Animals; Cell Line; Colorimetry / methods; Cricetinae; Cytopathogenic Effect, Viral; Dogs; Formazans / metabolism; Humans; Minute Virus of Mice / pathogenicity; Parvovirus, Canine / pathogenicity; Parvovirus, Porcine / pathogenicity; Reproducibility of Results; Sindbis Virus / pathogenicity; Swine; Tetrazolium Salts / metabolism; Viral Plaque Assay; Viruses / pathogenicity
TL;DR:
MTT has been applied to measure cytopathic effect produced by viral infection for different members of the parvovirus family and an unrelated virus, Sindbis, which is routinely titrated by plaque assay.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Crossref
Added: August 28, 2020
2006 journal article
Mutations in the endodomain of Sindbis virus glycoprotein E2 define sequences critical for virus assembly
JOURNAL OF VIROLOGY, 80(9), 4458–4468.
MeSH headings : Amino Acid Sequence; Animals; Capsid Proteins / genetics; Capsid Proteins / metabolism; Cell Line; Cricetinae; Gene Deletion; Microscopy, Electron, Transmission; Molecular Sequence Data; Mutation / genetics; Protein Structure, Tertiary; Sequence Alignment; Sindbis Virus / physiology; Sindbis Virus / ultrastructure; Tyrosine / genetics; Tyrosine / metabolism; Viral Envelope Proteins / chemistry; Viral Envelope Proteins / genetics; Viral Envelope Proteins / metabolism; Virus Assembly
TL;DR:
Submo acid substitutions and deletions in a region of the E2 tail (E2 amino acids 408 to 415) that is initially integrated into the endoplasmic reticulum are produced to define protein interactions critical for virus assembly and suggest a fundamental difference between Sindbis virus assembly in mammalian and insect cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
Sindbis virus infection of two model insect cell systems - A comparative study
Virus Research, 122(1-2), 28–34.
MeSH headings : Animals; Cell Line; Cell Shape; Cell Survival; Culicidae; Cytopathogenic Effect, Viral; Drosophila; Microscopy, Electron, Transmission; Sindbis Virus / growth & development; Viral Plaque Assay
TL;DR:
A comparative study of Sindbis virus infection of Schnieder-2 Drosophila (S2) cells to cells of the mosquito Aedes albopictus (clone U4.4) was undertaken, establishing S2 cells as an additional invertebrate model system to study the interactions of an invertebrates host with Sindbisirus.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: NC State University Libraries
Added: August 6, 2018
2006 journal article
Single and multiple deletions in the transmembrane domain of the Sindbis virus E2 glycoprotein identify a region critical for normal virus growth
VIROLOGY, 347(1), 199–207.
author keywords: Sindis virus; E2 glycoprotein; transmembrane domain; virus assembly; compensatory mutations
MeSH headings : Amino Acid Sequence; Animals; Cell Line; Cricetinae; Models, Molecular; Molecular Sequence Data; Protein Structure, Tertiary; Sequence Deletion; Sindbis Virus / genetics; Sindbis Virus / growth & development; Sindbis Virus / physiology; Viral Envelope Proteins / chemistry; Viral Envelope Proteins / genetics; Virus Replication / genetics
TL;DR:
Results suggest that the position of the deletion and the length of the C terminal region of the E2 transmembrane domain is vital for normal virus production, and deletion mutants resulting in decreased infectivity produce particles that appear to be processed and transported correctly suggesting a role involved in virus entry.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2005 journal article
In vivo processing and isolation of furin protease-sensitive alphavirus glycoproteins: a new technique for producing mutations in virus assembly
VIROLOGY, 332(2), 629–639.
author keywords: alphavirus; Sindbis; membrane glycoproteins; form protease; virus assembly
MeSH headings : Animals; Capsid Proteins / genetics; Cell Line; Cricetinae; Dithiothreitol / pharmacology; Ethylmaleimide / pharmacology; Furin / pharmacology; Kidney; Mutagenesis, Site-Directed; Mutagens; Sindbis Virus / drug effects; Sindbis Virus / genetics; Sindbis Virus / isolation & purification; Viral Envelope Proteins / genetics; Viral Structural Proteins / genetics
TL;DR:
In a new approach to the production of membrane-free membrane glycoproteins, furin protease recognition motifs were installed at various positions in the E1 glycoprotein ectodomain to provide a unique method for studying the mechanism of virus assembly and protein structure without altering crucial early events in protein assembly, folding, and maturation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2005 journal article
Single amino acid insertions at the junction of the Sindbis virus e2 transmembrane domain and endodomain disrupt virus envelopment and alter infectivity
Journal of Virology, 79(12), 7682–7697.
Source: NC State University Libraries
Added: August 6, 2018
2004 journal article
Conformational changes in Sindbis virions resulting from exposure to low pH and interactions with cells suggest that cell penetration may occur at the cell surface in the absence of membrane fusion
Virology, 324(2), 373–386.
Source: NC State University Libraries
Added: August 6, 2018
2004 journal article
Conformational changes in Sindbis virions resulting from exposure to low pH and interactions with cells suggest that cell penetration may occur at the cell surface in the absence of membrane fusion
Virology, 324(2), 373–386.
author keywords: Sindbis virus; electron cryo-microscopy; pH conditions; structure; infection; membrane fusion
MeSH headings : Animals; Cell Fusion; Cell Line; Cricetinae; Cryoelectron Microscopy; Hydrogen-Ion Concentration; Membrane Fusion; Microscopy, Electron; Protein Conformation; Sindbis Virus / chemistry; Sindbis Virus / physiology; Sindbis Virus / ultrastructure; Viral Envelope Proteins / chemistry; Viral Envelope Proteins / ultrastructure; Virion / chemistry; Virion / physiology; Virus Replication
TL;DR:
Investigation of the requirements for the induction of virus-mediated, low pH-induced cell-cell fusion and cell-virus fusion found that exposure to acid pH was required to establish conditions for membrane fusion but that membrane fusion did not occur until return to neutral pH.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Crossref
Added: October 9, 2021
2004 journal article
Controlling PVA hydrogels with gamma-cyclodextrin
MACROMOLECULES, 37(25), 9620–9625.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2004 journal article
Rapid preparative purification of West Nile and Sindbis virus PCR products utilizing a microbore anion-exchange column
Journal of Virological Methods, 120(2), 141–149.
Source: NC State University Libraries
Added: August 6, 2018
2004 journal article
Rapid preparative purification of West Nile and Sindbis virus PCR products utilizing a microbore anion-exchange column
Journal of Virological Methods, 120(2), 141–149.
author keywords: Sindbis virus; West Nile virus; PCR products; preparative anion-exchange HPLC purification
MeSH headings : Anions / analysis; Anions / isolation & purification; Chromatography, High Pressure Liquid; DNA, Viral / analysis; DNA, Viral / isolation & purification; Polymerase Chain Reaction / methods; Sindbis Virus / genetics; Sindbis Virus / isolation & purification; Time Factors; Virology / methods; West Nile virus / genetics; West Nile virus / isolation & purification
TL;DR:
This paper describes a simple and relatively inexpensive micro-preparative HPLC method to purify and analyze nM quantities of DNA that differs from existing methodologies because separation is based on size and charge as well as the overall G + C content of the PCR product.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Crossref
Added: October 9, 2021
2003 journal article
Deletions in the transmembrane domain of a Sindbis virus glycoprotein alter virus infectivity, stability, and host range
JOURNAL OF VIROLOGY, 77(23), 12710–12719.
MeSH headings : Amino Acid Sequence; Animals; Base Sequence; Cell Line; Cricetinae; DNA Primers; Lipid Bilayers; Molecular Sequence Data; Reverse Transcriptase Polymerase Chain Reaction; Sequence Deletion; Sequence Homology, Amino Acid; Sindbis Virus / genetics; Sindbis Virus / physiology; Virus Assembly
TL;DR:
It is found that progressive truncations in the transmembrane domain profoundly affected production of infectious virus in a cyclic fashion and also found that membrane composition effects protein-protein and protein- Membrane interactions during virus assembly.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2003 journal article
Morphological variants of Sindbis virus produced by a mutation in the capsid protein
VIROLOGY, 307(1), 54–66.
MeSH headings : Animals; Capsid Proteins / genetics; Cell Line; Cricetinae; Kidney; Microscopy, Electron; Mutation; RNA, Viral / genetics; Sindbis Virus / genetics; Sindbis Virus / physiology; Sindbis Virus / ultrastructure; Transcription, Genetic; Transfection; Viral Plaque Assay
TL;DR:
The data presented in the following manuscript show that mutations in the capsid protein can result in the assembly of the virus structural proteins into icosahedra of different triangulation numbers, and support the hypothesis that families of icosadeltahedra would be developed by altering the distance between the points of insertion of the five-fold axis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2001 journal article
Exposure to low pH is not required for penetration of mosquito cells by Sindbis virus
JOURNAL OF VIROLOGY, 75(4), 2010–2013.
MeSH headings : Aedes / virology; Ammonium Chloride / pharmacology; Animals; Cells, Cultured; Chloroquine / pharmacology; Hydrogen-Ion Concentration; Sindbis Virus / drug effects; Sindbis Virus / physiology; Virus Replication / drug effects
TL;DR:
The results suggest that while these two drugs raise the pH of endosomes, they do not block insect cell penetration, and support previous results suggesting that exposure to an acid environment within the cell may not be an obligatory step in the process of infection of cells by alphaviruses.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2001 journal article
Locations of carbohydrate sites on alphavirus glycoproteins show that E1 forms an icosahedral scaffold
CELL, 105(1), 127–136.
MeSH headings : Alphavirus / chemistry; Alphavirus / genetics; Alphavirus / ultrastructure; Amino Acid Motifs / genetics; Animals; Binding Sites / genetics; Carbohydrates / chemistry; Cell Line; Cricetinae; Cryoelectron Microscopy; Encephalitis Viruses, Tick-Borne / chemistry; Glycosylation; Membrane Glycoproteins / chemistry; Membrane Glycoproteins / genetics; Models, Molecular; Mutagenesis, Site-Directed; Protein Conformation; Ross River virus / chemistry; Sindbis Virus / chemistry; Sindbis Virus / ultrastructure; Viral Envelope Proteins / chemistry; Viral Envelope Proteins / genetics; Viral Fusion Proteins / chemistry; Viral Fusion Proteins / genetics
TL;DR:
There are 80 spikes on the surface of Sindbis virus arranged as an icosahedral surface lattice, consistent with the observed structural changes that occur on fusion with host cell membranes, suggesting a similar fusion mechanism for alpha- and flaviviruses.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2000 journal article
A single deletion in the membrane-proximal region of the Sindbis virus glycoprotein E2 endodomain blocks virus assembly
JOURNAL OF VIROLOGY, 74(9), 4220–4228.
MeSH headings : Amino Acid Sequence; Animals; Binding Sites; Biological Transport; Cell Line; Cell Membrane / metabolism; Cricetinae; Cytoplasm / metabolism; Humans; Intracellular Fluid / metabolism; Molecular Sequence Data; Mutagenesis; Nucleocapsid / metabolism; Sindbis Virus / genetics; Sindbis Virus / physiology; Sindbis Virus / ultrastructure; Viral Envelope Proteins / genetics; Viral Envelope Proteins / metabolism; Viral Envelope Proteins / physiology; Viral Structural Proteins / metabolism; Virus Assembly / physiology
TL;DR:
A deletion mutant is constructed, ΔK391, in which a nonconserved lysine at the membrane-cytoplasm junction of the E2 tail has been deleted and it is proposed that the phenotypic differences displayed by this mutant in the two diverse host cells arise from fundamental differences in the lipid composition of the insect cell membranes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
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