@article{box_dunnagan_hirsh_cherry_christianson_gibson_wolfe_gallardo-williams_2017, title={Qualitative and Quantitative Evaluation of Three Types of Student Generated Videos as Instructional Support in Organic Chemistry Laboratories}, volume={94}, ISSN={["1938-1328"]}, DOI={10.1021/acs.jchemed.6b00451}, abstractNote={This study was designed to evaluate the effectiveness of student-generated videos as a supplement to teaching assistant (TA) instruction in an undergraduate organic chemistry laboratory. Three videos covering different aspects of lab instruction (experimental technique, use of instrumentation, and calculations) were produced using student-generated scripts. A laboratory classroom was outfitted with video cameras and sound recording equipment that allowed the research team to monitor all TA–student and student–student interactions. Six course sections led by three randomly assigned TAs were selected. Two sections from each TA were observed (control and treatment), each at the same time of day, 1 week apart. Students in the control group had their TA conduct the lab briefing and supervise the lab, but were given no access to the instructional videos. The treatment group had videos available to supplement the TA’s lab briefing but was otherwise identical to the control group. Both groups were given a questionnaire that contained two comprehension questions per category to be completed during the lab before performing the experiment. Statistical analysis of the responses to this pre-experimental questionnaire showed that students who watched the videos had a better understanding of the methods than the students in sections that only received the TA lab briefing. Effect size calculations using Cohen’s d indicate that the Instrumentation video had a large positive effect on the number of correct responses in the treatment groups, while small effects were found for the Technique and Calculation videos. Content analysis of the lab transcripts supports these findings. In addition to these effects, treatment groups invariably completed the lab in less time than the control groups. Results from a follow-up survey e-mailed to students the week after their lab session show that most students found the videos to be valuable when completing the lab, with the Technique video being generally ranked as most helpful.}, number={2}, journal={JOURNAL OF CHEMICAL EDUCATION}, publisher={American Chemical Society (ACS)}, author={Box, Melinda C. and Dunnagan, Cathi L. and Hirsh, Lauren A. S. and Cherry, Clinton R. and Christianson, Kayla A. and Gibson, Radiance J. and Wolfe, Michael I. and Gallardo-Williams, Maria T.}, year={2017}, month={Feb}, pages={164–170} }
 @article{nepomuceno_gibson_randall_muddiman_2014, title={Accurate Identification of Deamidated Peptides in Global Proteomics Using a Quadrupole Orbitrap Mass Spectrometer}, volume={13}, ISSN={["1535-3907"]}, DOI={10.1021/pr400848n}, abstractNote={Deamidation of asparagine and glutamine residues is a common post-translational modification. Researchers often rely on mass spectrometric based proteomic techniques for the identification of these post-translational sites. Mass spectral analysis of deamidated peptides is complicated and often misassigned due to overlapping (13)C peak of the amidated form with the deamidated monoisotopic peak; these two peaks are only separated by 19.34 mDa. For proper assignment, it is inherently important to use a mass spectrometer with high mass measurement accuracy and high resolving power. Herein, mouse brain tissue lysate was prepared using filter-aided sample preparation (FASP) method and Stage Tip fractionation followed by analysis on a nanoLC coupled with a quadrupole orbitrap (Q-Exactive) mass spectrometer to accurately identify more than 5400 proteins. Mass spectral data was processed using MASCOT and ProteoIQ for accurate identification of peptides and proteins. MASCOT search values for precursor and MS/MS mass tolerances were investigated, and it was determined that data searched with greater than 5 ppm precursor mass tolerance resulted in the misassignment of deamidated peptides. Peptides that were identified with a mass measurement accuracy of ±5 ppm were correctly assigned.}, number={2}, journal={JOURNAL OF PROTEOME RESEARCH}, author={Nepomuceno, Angelito I. and Gibson, Radiance J. and Randall, Shan M. and Muddiman, David C.}, year={2014}, month={Feb}, pages={777–785} }