@article{brannan_wysocky_anderson_2024, title={Effect of Saccharomyces cerevisiae dietary supplementation (Original XPC TM ) and stocking density on production performance and egg quality in white strain hens}, volume={33}, ISSN={["1537-0437"]}, url={https://doi.org/10.1016/j.japr.2024.100423}, DOI={10.1016/j.japr.2024.100423}, abstractNote={The ongoing industry trend towards less intensive management systems is of interest to table egg producers, with antibiotic usage and stocking density being of primary concern. The present trial evaluated the effect of a Saccharomyces cerevisiae fermentation product and stocking density on hen production performance and egg quality. Eleven white egg layer strains were equally represented in cages at either high (471 cm2 per bird, HD) or low (497 cm2 per bird, LD) stocking densities. Hens were fed either a control diet (CN) or one supplemented with S. cerevisiae (Original XPCTM included at 0.68 kg/T, SC) from 17 to 41 weeks on a commercially relevant scale (70 replicates for each of the four treatments and a total of 5,740 hens). Data were collected every four weeks and analyzed in a 2 × 2 factorial design, with stocking density and diet being the main effects. Feed intake and egg production were increased in both the LD and SC groups. Improvements in egg size were also noted for SC hens, concurrent with a reduction in cracks, leaks, and vitelline elasticity. The combination of SC-LD resulted in improved hen day production, shorter time to 50% production, and increased percentage of large eggs. Overall, the main effects of SC and LD were associated with improved production performance, with larger egg size also being noted for the SC hens. The combination of SC and LD may benefit producers in achieving both production and egg size performance targets, providing an economic incentive to transition to alternative management systems.}, number={2}, journal={JOURNAL OF APPLIED POULTRY RESEARCH}, author={Brannan, Kelly E. and Wysocky, R. and Anderson, Kenneth E.}, year={2024}, month={Jun} }
 @article{toomer_vu_wysocky_moraes_malheiros_anderson_2021, title={The Effect of Feeding Hens a Peanut Skin-Containing Diet on Hen Performance, and Shell Egg Quality and Lipid Chemistry}, volume={11}, ISSN={["2077-0472"]}, DOI={10.3390/agriculture11090894}, abstractNote={Peanut skins are a considerable waste product with little current economic value or use. We aimed to determine the dietary effects of peanut skins on layer production performance and egg quality and chemistry of the eggs produced. Two hundred commercial hens were randomly assigned to four treatments (five replicates) and fed ad libitum for 8 weeks: conventional control diet, diet containing 24% high-oleic peanut (HOPN), diet containing 3% peanut skin (PN Skin), and a diet with 2.5% oleic acid (OA). Hens fed the HOPN diet had significantly reduced body weights relative to the control and PN Skin treatments, producing fewer total eggs over the 8-week experimental period. Eggs weights were similar between the control and PN Skin treatments at weeks 2 and 4, while eggs from the PN Skin treatment group were heavier than other treatments at weeks 6 and 8 of the experiment. Eggs produced from the HOPN treatment had reduced saturated fatty acid (FA) content in comparison to the other treatment groups, while similar between PN Skin and control eggs at week 8 of the experiment. This study suggests that PN skins may be a suitable alternative layer feed ingredient.}, number={9}, journal={AGRICULTURE-BASEL}, author={Toomer, Ondulla and Vu, Thien and Wysocky, Rebecca and Moraes, Vera and Malheiros, Ramon and Anderson, Kenneth}, year={2021}, month={Sep} }
 @article{harding_vu_wysocky_malheiros_anderson_toomer_2021, title={The Effects of Feeding a Whole-in-Shell Peanut-Containing Diet on Layer Performance and the Quality and Chemistry of Eggs Produced}, volume={11}, ISSN={["2077-0472"]}, DOI={10.3390/agriculture11111176}, abstractNote={The abundance of peanut and poultry production within the state of North Carolina and the US Southeast, led us to conduct a layer feeding trial to determine the utilization of whole-in-shell high-oleic peanuts (WPN) and/or unblanched high-oleic peanuts (HOPN) as an alternative feed ingredient for poultry. To meet this objective, we randomly assigned 576 shaver hens to 4 dietary treatments (4 rep/trt). The dietary treatments consisted of a conventional control diet (C1), a diet containing 4% WPN, an 8% HOPN diet, and a control diet containing soy protein isolate (C2). Feed and water were provided for 6 weeks ad libitum. Pen body weights (BW) were recorded at week 0 and week 6 (wk6), and feed weights were recorded bi-weekly. Shell eggs were collected daily and enumerated. Bi-weekly 120 eggs/treatment were collected for quality assessment and egg weight (EW), while 16 eggs/treatment were collected for chemical analysis. There were no significant differences in BW or EW at week 6. Hens fed the C2 produced more total dozen eggs relative to C1 hens over the feeding trial (p < 0.05). Hens fed the C1 diet consumed less total feed relative to the other treatments with the best feed conversion ratio (p < 0.05). Most eggs produced from each treatment were USDA grade A, large eggs. There were no differences in egg quality, with the exception of yolk color, with significantly higher yolk color scores in eggs produced from the C1 and C2 treatments relative to the other treatments (p < 0.05). Eggs produced from the HOPN treatment had significantly reduced stearic and linoleic fatty acid levels relative to the other treatments (p < 0.05). Eggs produced from hens fed the WPN diet had significantly greater β-carotene content relative to eggs from the other treatment groups (p < 0.05). In summary, this study suggests that WPN and/or HOPN may be a suitable alternative layer feed ingredient and a dietary means to enrich the eggs produced while not adversely affecting hen performance.}, number={11}, journal={AGRICULTURE-BASEL}, author={Harding, Kari L. and Vu, Thien and Wysocky, Rebecca and Malheiros, Ramon and Anderson, Kenneth E. and Toomer, Ondulla T.}, year={2021}, month={Nov} }
 @article{hawkridge_wysocky_petitte_anderson_mozdziak_fletcher_horowitz_muddiman_2010, title={Measuring the intra-individual variability of the plasma proteome in the chicken model of spontaneous ovarian adenocarcinoma}, volume={398}, ISSN={["1618-2650"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-77957867631&partnerID=MN8TOARS}, DOI={10.1007/s00216-010-3979-y}, abstractNote={The domestic chicken (Gallus domesticus) has emerged as a powerful experimental model for studying the onset and progression of spontaneous epithelial ovarian cancer (EOC) with a disease prevalence that can exceed 35% between 2 and 7 years of age. An experimental strategy for biomarker discovery is reported herein that combines the chicken model of EOC, longitudinal plasma sample collection with matched tissues, advanced mass spectrometry-based proteomics, and concepts derived from the index of individuality (Harris, Clin Chem 20: 1535-1542, 1974). Blood was drawn from 148 age-matched chickens starting at 2.5 years of age every 3 months for 1 year. At the conclusion of the 1 year sample collection period, the 73 birds that remained alive were euthanized, necropsied, and tissues were collected. Pathological assessment of resected tissues from these 73 birds confirmed that five birds (6.8%) developed EOC. A proteomics workflow including in-gel digestion, nanoLC coupled to high-performance mass spectrometry, and label-free (spectral counting) quantification was used to measure the biological intra-individual variability (CV(W)) of the chicken plasma proteome. Longitudinal plasma sample sets from two birds within the 73-bird biorepository were selected for this study; one bird was considered "healthy" and the second bird developed late-stage EOC. A total of 116 proteins from un-depleted plasma were identified with 80 proteins shared among all sample sets. Analytical variability (CV(A)) of the label-free proteomics workflow was measured using a single plasma sample analyzed five times and was found to be ≥CV(W) in both birds for 16 proteins (20%) and in either bird for 25 proteins (31%). Ovomacroglobulin (ovostatin) was found to increase (p < 0.001) over a 6 month period in the late-stage EOC bird providing an initial candidate protein for further investigation.}, number={2}, journal={ANALYTICAL AND BIOANALYTICAL CHEMISTRY}, author={Hawkridge, Adam M. and Wysocky, Rebecca B. and Petitte, James N. and Anderson, Kenneth E. and Mozdziak, Paul E. and Fletcher, Oscar J. and Horowitz, Jonathan M. and Muddiman, David C.}, year={2010}, month={Sep}, pages={737–749} }
 @article{hawkridge_wysocky_petitte_anderson_mozdziak_fletcher_horowitz_muddiman_2010, title={Measuring the intra-individual variability of the plasma proteome in the chicken model of spontaneous ovarian adenocarcinoma (vol 398, pg 737, 2010)}, volume={398}, ISSN={["1618-2642"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-77957848897&partnerID=MN8TOARS}, DOI={10.1007/s00216-010-4107-8}, number={4}, journal={ANALYTICAL AND BIOANALYTICAL CHEMISTRY}, author={Hawkridge, Adam M. and Wysocky, Rebecca B. and Petitte, James N. and Anderson, Kenneth E. and Mozdziak, Paul E. and Fletcher, Oscar J. and Horowitz, Jonathan M. and Muddiman, David C.}, year={2010}, month={Oct}, pages={1835–1835} }