Works (8)

Updated: July 20th, 2023 21:17

2020 article

Mutant Construction and Integration Vector-Mediated Genetic Complementation in Listeria monocytogenes

LISTERIA MONOCYTOGENES, 2 EDITION, Vol. 2220, pp. 177–185.

By: C. Parsons n, R. Azizoglu n, D. Elhanafi n & S. Kathariou n

author keywords: Transposon; Mutant; Listeria monocytogenes; Complementation
MeSH headings : Electroporation / methods; Genetic Vectors / genetics; Humans; Listeria monocytogenes / genetics; Listeriosis / microbiology; Mutagenesis, Insertional / methods; Mutation; Plasmids / genetics; Polymerase Chain Reaction / methods; Retroelements
TL;DR: This chapter describes the construction and screening of mutant libraries of L. monocytogenes using the plasmid pMC38, carrying a mariner-based transposon system (TC1/mariner) and constructed by Cao et al. (via Semantic Scholar)
Source: Web Of Science
Added: August 30, 2021

2012 journal article

Bovine Staphylococcus aureus: Dose response to iodine and chlorhexidine and effect of iodine challenge on antibiotic susceptibility

JOURNAL OF DAIRY SCIENCE, 96(2), 993–999.

By: R. Azizoglu n, R. Lyman n & K. Anderson n

author keywords: Staphylococcus aureus; disinfectant tolerance; mastitis; antibiotic stress
MeSH headings : Animals; Anti-Infective Agents, Local / administration & dosage; Anti-Infective Agents, Local / therapeutic use; Cattle; Chlorhexidine / administration & dosage; Chlorhexidine / therapeutic use; Dose-Response Relationship, Drug; Drug Resistance, Bacterial / drug effects; Female; Iodine / administration & dosage; Iodine / therapeutic use; Mammary Glands, Animal / microbiology; Mastitis, Bovine / drug therapy; Mastitis, Bovine / microbiology; Mastitis, Bovine / prevention & control; Microbial Sensitivity Tests / veterinary; Staphylococcal Infections / drug therapy; Staphylococcal Infections / microbiology; Staphylococcal Infections / veterinary; Staphylococcus aureus / drug effects
TL;DR: The minimum inhibitory concentrations of iodine were determined for 37 isolates of Staph. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Purine Biosynthesis Mutants (purA and purB) of Serotype 4b Listeria monocytogenes Are Severely Attenuated for Systemic Infection in Intragastrically Inoculated A/J Mice

FOODBORNE PATHOGENS AND DISEASE, 9(5), 480–486.

By: N. Faith*, J. Kim n, R. Azizoglu n, S. Kathariou n & C. Czuprynski*

MeSH headings : Adenylosuccinate Synthase / genetics; Adenylosuccinate Synthase / metabolism; Animals; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Bacterial Translocation; Caco-2 Cells; Female; Gallbladder / microbiology; Gastric Juice / chemistry; Gastroenteritis / microbiology; Gastroenteritis / physiopathology; Humans; Intestinal Mucosa / microbiology; Isoenzymes / genetics; Isoenzymes / metabolism; Listeria monocytogenes / genetics; Listeria monocytogenes / metabolism; Listeria monocytogenes / pathogenicity; Listeria monocytogenes / physiology; Listeriosis / microbiology; Listeriosis / physiopathology; Liver / microbiology; Mice; Mice, Inbred A; Microbial Viability; Mutant Proteins / metabolism; Purines / biosynthesis; Spleen / microbiology; Virulence
TL;DR: It is demonstrated that purA and purB transposon mutants of serotype 4b Listeria monocytogenes were severely impaired in their ability to colonize the gastrointestinal tract and cause systemic infection of the spleen, liver, and gallbladder following intragastric inoculation of A/J mice. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Impact of Growth Temperature and Agar Versus Liquid Media on Freeze-Thaw Tolerance of Yersinia enterocolitica

FOODBORNE PATHOGENS AND DISEASE, 7(9), 1125–1128.

By: R. Azizoglu n & S. Kathariou n

MeSH headings : Agar; Culture Media; Food Microbiology; Food Preservation / methods; Freezing; Hot Temperature; Temperature; Yersinia enterocolitica / growth & development
TL;DR: The data suggest species-specific impact of growth temperature and liquid versus agar medium on cryotolerance of cold-tolerant bacteria is a general adaptation shared with other foodborne pathogens. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Inactivation of a Cold-Induced Putative RNA Helicase Gene of Listeria monocytogenes Is Accompanied by Failure To Grow at Low Temperatures but Does Not Affect Freeze-Thaw Tolerance

JOURNAL OF FOOD PROTECTION, 73(8), 1474–1479.

By: R. Azizoglu n & S. Kathariou n

MeSH headings : Adaptation, Physiological; Cold Temperature; Consumer Product Safety; DNA Transposable Elements / genetics; Food Microbiology; Freezing; Listeria monocytogenes / genetics; Listeria monocytogenes / growth & development; RNA Helicases / genetics; Temperature
TL;DR: The findings suggest that the transposon insertion in the putative helicase gene, in spite of the severely cold-sensitive phenotype that accompanies it, does not affect the ability of the bacteria to cope with cold-related stresses encountered during repeated freezing and thawing. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Temperature-Dependent Requirement for Catalase in Aerobic Growth of Listeria monocytogenes F2365

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(21), 6998–7003.

By: R. Azizoglu n & S. Kathariou n

MeSH headings : Aerobiosis / physiology; Animals; Catalase / genetics; Catalase / metabolism; Catalase / physiology; Chick Embryo / microbiology; Culture Media; Food Microbiology; Freezing; Listeria monocytogenes / enzymology; Listeria monocytogenes / genetics; Listeria monocytogenes / growth & development; Listeria monocytogenes / physiology; Mutation; Temperature
TL;DR: In spite of the expected impact of oxidative stress responses on cryotolerance, cryot tolerance of the kat mutant was not affected, and inactivation of this gene was responsible for the growth impairment. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Role of Growth Temperature in Freeze-Thaw Tolerance of Listeria spp.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(16), 5315–5320.

By: R. Azizoglu*, J. Osborne n, S. Wilson* & S. Kathariou*

MeSH headings : Adaptation, Physiological; Agar; Animals; Cold Temperature; Colony Count, Microbial; Culture Media; Disease Outbreaks; Environmental Microbiology; Food-Processing Industry; Freezing; Gene Expression Regulation, Bacterial; Heat-Shock Response; Humans; Listeria / classification; Listeria / growth & development; Listeria monocytogenes / growth & development; Listeriosis / epidemiology; Listeriosis / microbiology; Plankton / growth & development; Temperature
TL;DR: It is suggested that growth at 37°C, a temperature required for expression of virulence determinants of L. monocytogenes, is also required for protection against freeze-thaw stress. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Impact of antibiotic stress on acid and heat tolerance and virulence factor expression of Escherichia coli O157 : H7

JOURNAL OF FOOD PROTECTION, 70(1), 194–199.

By: R. Azizoglu n & M. Drake n

MeSH headings : Adaptation, Physiological; Anti-Bacterial Agents / pharmacology; Colony Count, Microbial; Consumer Product Safety; Dose-Response Relationship, Drug; Escherichia coli O157 / genetics; Escherichia coli O157 / growth & development; Escherichia coli O157 / pathogenicity; Food Microbiology; Gastric Acid; Gene Expression Regulation, Bacterial; Hot Temperature; Humans; Hydrochloric Acid / pharmacology; Hydrogen-Ion Concentration; Microbial Sensitivity Tests; Virulence Factors / genetics; Virulence Factors / metabolism
TL;DR: This study was conducted to determine the effect of antibiotic stress on the virulence factor expression, simulated gastric fluid survival, and heat tolerance of Escherichia coli O157:H7 and found that antibiotics do not produce a cross-protective response to heat or decreased pH. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

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