Works (54)

Updated: July 16th, 2023 21:18

2020 journal article

An Efficient Stevia rebaudiana Transformation System and In vitro Enzyme Assays Reveal Novel Insights into UGT76G1 Function

SCIENTIFIC REPORTS, 10(1).

By: Q. Wu n, C. La Hovary n, H. Chen n, X. Li n, H. Eng n, V. Vallejo*, R. Qu n, R. Dewey n

MeSH headings : Alternative Splicing; Glycosyltransferases / biosynthesis; Glycosyltransferases / genetics; Isoenzymes / biosynthesis; Isoenzymes / genetics; Plant Proteins / biosynthesis; Plant Proteins / genetics; Plants, Genetically Modified / enzymology; Plants, Genetically Modified / genetics; Stevia / enzymology; Stevia / genetics; Transformation, Genetic
TL;DR: A highly efficient Agrobacterium-mediated stable transformation system using axillary shoots as the initial explant is reported and evidence suggesting that alternative and/or aberrant splicing may serve to influence the ability of the plant to produce functional UGT76G1 transcripts, and possibly produce enzyme variants within the plant is found. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: September 14, 2020

2018 journal article

Acetosyringone treatment duration affects large T-DNA molecule transfer to rice callus

BMC BIOTECHNOLOGY, 18.

By: J. Xi n, M. Patel n, S. Dong*, Q. Que* & R. Qu n

author keywords: Agrobacterium; BIBAC; Immuno-precipitation; Large T-DNA; Monocot transformation
MeSH headings : Acetophenones / pharmacology; Agrobacterium tumefaciens / genetics; Chromosomes, Artificial, Bacterial / genetics; DNA, Bacterial / metabolism; Oryza / genetics; Plants, Genetically Modified / genetics; Transformation, Genetic / drug effects
TL;DR: It is demonstrated that extension of AS induction time highly significantly improved large-size T- DNA transfer to rice cells and provided valuable information of the T-strand dynamics and its impact on large T-DNA transfer in monocots, and likely dicots as well. (via Semantic Scholar)
Source: Web Of Science
Added: October 19, 2018

2018 journal article

Cholesterol accumulation by suppression of SMT1 leads to dwarfism and improved drought tolerance in herbaceous plants

PLANT CELL AND ENVIRONMENT, 41(6), 1417–1426.

author keywords: cholesterol; drought tolerance; plant growth; polyamine synthesis; sterol-C24-methyltransferase 1
MeSH headings : Adaptation, Physiological / genetics; Cholesterol / metabolism; Cynodon / anatomy & histology; Cynodon / genetics; Cynodon / physiology; Down-Regulation / genetics; Droughts; Gene Expression Regulation, Plant; Mutation / genetics; Oryza / anatomy & histology; Oryza / genetics; Oryza / physiology; Plant Proteins / metabolism; Plants, Genetically Modified; Polyamines / metabolism; RNA Interference; RNA, Messenger / genetics; RNA, Messenger / metabolism; Suppression, Genetic
TL;DR: It is suggested that cholesterol induces expression of SAMDC and leads to dwarfism and elevated drought tolerance in plants as a result of the promoted Spd and Spm synthesis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2018 journal article

Effects of overexpression of jasmonic acid biosynthesis genes on nicotine accumulation in tobacco

Plant Direct, 2(1), e00036.

By: H. Chen n, B. Wang*, S. Geng*, C. Arellano n, S. Chen* & R. Qu n

author keywords: jasmonic acid biosynthesis; jasmonic acid signaling; nicotine synthesis regulation; tobacco; transgene
TL;DR: The research revealed a tightly controlled JA signaling pathway and a complicated regulatory network for nicotine biosynthesis by JA signaling and nicotine content in these transgenic plants did not increase. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: Crossref
Added: February 24, 2020

2017 journal article

Accumulation of medium-chain, saturated fatty acyl moieties in seed oils of transgenic Camelina sativa

PLOS ONE, 12(2).

Contributors: Z. Hu n, Q. Wu n, J. Dalal n, N. Vasani n, H. Lopez n, H. Sederoff n, R. Qu n

MeSH headings : 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase / deficiency; 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase / genetics; Brassicaceae / genetics; Brassicaceae / metabolism; Plant Oils / metabolism; Plants, Genetically Modified; RNA Interference; Seeds / metabolism; Thiolester Hydrolases / genetics; Triglycerides / chemistry; Triglycerides / metabolism; Umbellularia / enzymology; Umbellularia / genetics
TL;DR: The results show that a California bay gene can be successfully used to modify the oil composition in camelina seed and present a new biological alternative for jet fuel production. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2017 journal article

Evaluation of parameters affecting switchgrass tissue culture: toward a consolidated procedure for Agrobacterium-mediated transformation of switchgrass (Panicum virgatum)

PLANT METHODS, 13.

By: C. Lin*, B. Donohoe*, N. Ahuja*, D. Garrity*, R. Qu n, M. Tucker*, M. Himmel*, H. Wei*

author keywords: Switchgrass; Bioenergy crop; Red fluorescent protein (RFP); ss-Glucuronidase (GUS) staining; Agrobacterium-mediated transformation
TL;DR: The optimization of seed sterilization, callus induction, and regeneration steps, a reliable and effective protocol is established to facilitate switchgrass engineering and provide an improved strategy to promote the production of callus and generation of transgenic switchgrass lines. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Control of brown patch (Rhizoctonia solani) in tall fescue (Festuca arundinacea Schreb.) by host induced gene silencing

PLANT CELL REPORTS, 35(4), 791–802.

By: B. Zhou n, A. Bailey*, C. Niblett* & R. Qu n

author keywords: Brown patch; Disease resistance; HIGS; Rhizoctonia solani; RNAi; Tall fescue
MeSH headings : Blotting, Northern; Blotting, Southern; Databases, Genetic; Disease Resistance; Festuca / microbiology; Gene Silencing; Genes, Essential; Genes, Fungal; Host-Pathogen Interactions; Plant Diseases / microbiology; Plants, Genetically Modified; Polymerase Chain Reaction; RNA, Small Interfering / metabolism; Rhizoctonia / genetics; Rhizoctonia / physiology; Saccharomyces cerevisiae / genetics; Transgenes
TL;DR: This research demonstrates resistance to brown patch disease achieved by the application of host induced gene silencing and is the first case that RNAi constructs of pathogen genes introduced into a host plant can confer resistance against a necrotrophic fungus. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Expression of the shrimp antimicrobial peptide penaeidin 4-1 confers resistance against brown patch disease in tall fescue

PLANT CELL TISSUE AND ORGAN CULTURE, 125(3), 599–603.

By: B. Zhou n, H. Luo* & R. Qu n

author keywords: Brown patch; Disease resistance; Penaeidin; Rhizoctonia solani subgroups; Tall fescue
TL;DR: The results indicate that Pen4-1 confers resistance to various subgroups of R. solani and is a promising candidate for engineered resistance to a wide-spectrum pathogens in tall fescue. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2015 journal article

A novel gateway-compatible binary vector series (PC-GW) for flexible cloning of multiple genes for genetic transformation of plants

PLASMID, 81, 55–62.

By: J. Dalal n, R. Yalamanchili n, C. Hovary n, M. Ji n, M. Rodriguez-Welsh n, D. Aslett n, S. Ganapathy n, A. Grunden n ...

Contributors: J. Dalal n, R. Yalamanchili n, C. La Hovary n, M. Ji n, M. Rodriguez-Welsh n, D. Aslett n, S. Ganapathy n, A. Grunden n, H. Sederoff n, R. Qu n

author keywords: Gateway; Plant transformation; Agrobacterium; Gene stacking
MeSH headings : Cloning, Molecular / methods; Gene Expression; Gene Order; Genes, Reporter; Genetic Vectors / genetics; Plants, Genetically Modified; Plasmids / genetics; Transformation, Genetic; Transgenes
TL;DR: The PC-GW vectors provide choices for selectable markers, cloning methods, and can accommodate up to eight gene constructs in a single T-DNA, thereby significantly reducing the number of transformations or crosses needed to generate multi-transgene expressing plants. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2015 journal article

A photorespiratory bypass increases plant growth and seed yield in biofuel crop Camelina sativa

BIOTECHNOLOGY FOR BIOFUELS, 8(1).

By: J. Dalal n, H. Lopez n, N. Vasani n, Z. Hu n, J. Swift n, R. Yalamanchili n, M. Dvora n, X. Lin n ...

Contributors: J. Dalal n, H. Lopez n, N. Vasani n, Z. Hu n, J. Swift n, R. Yalamanchili n, M. Dvora n, X. Lin n ...

author keywords: Camelina; Seed yield; Biofuel; Photorespiratory bypass; Photosynthesis
TL;DR: By reducing photorespiratory losses and increasing photosynthetic CO2 fixation rates, transgenic plants show dramatic increases in seed yield, and the bypass approach may have significant impact on increasing agricultural productivity for C3 crops. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2015 journal article

Genetic Factors for Enhancement of Nicotine Levels in Cultivated Tobacco

SCIENTIFIC REPORTS, 5.

MeSH headings : Nicotine / biosynthesis; Nicotine / genetics; Plant Leaves / genetics; Plant Leaves / metabolism; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Tobacco / genetics; Tobacco / metabolism; Transcription Factors / genetics; Transcription Factors / metabolism
TL;DR: It is demonstrated that the increased nicotine trait was stably inherited to the T2 and T3 generations, indicating the important role that Nt MYC2a plays in regulating nicotine accumulation in N. tabacum and the great potential of NtMYC 2a overexpression in tobacco plants for industrial nicotine production. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Overexpression of ubiquitin-like LpHUB1 gene confers drought tolerance in perennial ryegrass

PLANT BIOTECHNOLOGY JOURNAL, 13(5), 689–699.

By: M. Patel n, S. Milla-Lewis n, W. Zhang n, K. Templeton*, W. Reynolds n, K. Richardson*, M. Biswas*, M. Zuleta n ...

author keywords: drought tolerance; HUB1; Lolium perenne; small ORF; transgenic
MeSH headings : Base Sequence; Chlorophyll / metabolism; Droughts; Gene Expression; Gene Expression Regulation, Plant; Lolium / genetics; Lolium / physiology; Molecular Sequence Data; Plant Leaves / genetics; Plant Leaves / physiology; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified; Sequence Analysis, DNA; Stress, Physiological; Ubiquitin / genetics; Ubiquitin / metabolism; Water / physiology
TL;DR: Improved drought tolerance in pasture-type and turf-type transgenic perennial ryegrass plants overexpressing LpHUB1 showed improved drought tolerance, as evidenced by improved turf quality, maintenance of turgor and increased growth. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2014 journal article

Tissue culture, genetic transformation, and improvement of switchgrass through genetic engineering

Compendium of Bioenergy Plants: Switchgrass, 253–293.

By: B. Zhao, R. Qu, R. Li, B. Xu & T. Frazier

Source: NC State University Libraries
Added: August 6, 2018

2013 journal article

Enhanced Agrobacterium-mediated transformation efficiencies in monocot cells is associated with attenuated defense responses

PLANT MOLECULAR BIOLOGY, 81(3), 273–286.

By: W. Zhang n, R. Dewey n, W. Boss n, B. Phillippy n & R. Qu n

author keywords: Cold shock; Glutamine; myo-inositol; Plant defense response; Transformation
MeSH headings : Agrobacterium tumefaciens / genetics; Agrobacterium tumefaciens / physiology; Arabidopsis / drug effects; Arabidopsis / genetics; Arabidopsis / immunology; Arabidopsis / physiology; Cell Wall / metabolism; Cold Temperature; Culture Media; Gene Transfer Techniques; Genetic Vectors; Glutamine / pharmacology; Green Fluorescent Proteins; Hydrogen Peroxide / metabolism; Inositol / pharmacology; Lignin / metabolism; Lolium / drug effects; Lolium / genetics; Lolium / immunology; Lolium / physiology; Oryza / drug effects; Oryza / genetics; Oryza / immunology; Oryza / physiology; Phytic Acid / metabolism; Plant Immunity; Plant Roots / drug effects; Plant Roots / genetics; Plant Roots / immunology; Plant Roots / physiology; Plants, Genetically Modified; Regeneration; Seeds / drug effects; Seeds / genetics; Seeds / immunology; Seeds / physiology; Starch / metabolism; Tissue Culture Techniques; Transformation, Genetic
TL;DR: The utilization of culture conditions associated with an attenuation of defense responses in monocot plant cells led to highly improved Agrobacterium-mediated transformation efficiencies in perennial ryegrass (Lolium perenne L.). (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Enhancing Agrobacterium tumefaciens-mediated transformation efficiency of perennial ryegrass and rice using heat and high maltose treatments during bacterial infection

PLANT CELL TISSUE AND ORGAN CULTURE, 114(1), 19–29.

By: M. Patel n, R. Dewey n & R. Qu n

author keywords: Heat shock; Maltose; Rice; Perennial ryegrass; Transformation
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Plant regeneration and genetic transformation of C. canadensis: a non-model plant appropriate for investigation of flower development in Cornus (Cornaceae)

Plant Cell Reports, 32(1), 77–87.

By: X. Liu n, C. Feng n, R. Franks n, R. Qu n, D. Xie n & Q. Xiang n

Contributors: X. Liu n, C. Feng n, R. Franks n, R. Qu n, D. Xie n & Q. Xiang n

MeSH headings : Cornus / drug effects; Cornus / genetics; Cornus / growth & development; Flowers / drug effects; Flowers / genetics; Flowers / growth & development; Fluorescence; Gene Expression Regulation, Plant; Green Fluorescent Proteins / metabolism; Hygromycin B / pharmacology; Inflorescence / drug effects; Inflorescence / growth & development; Microscopy, Confocal; Models, Biological; Plant Shoots / drug effects; Plant Shoots / growth & development; Regeneration / drug effects; Reverse Transcriptase Polymerase Chain Reaction; Soil; Transformation, Genetic / drug effects
TL;DR: An effective protocol of plant regeneration and genetic transformation of C. canadensis via Agrobacterium strain EHA105-mediated transformation is reported, providing an appropriate platform to investigate the molecular mechanisms by which the various inflorescence forms are developed in Cornus plants. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science; OpenAlex)
Sources: NC State University Libraries, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

2012 journal article

Dilute sulfuric acid pretreatment of transgenic switchgrass for sugar production

BIORESOURCE TECHNOLOGY, 104, 823–827.

By: X. Zhou, J. Xu n, Z. Wang n, J. Cheng n, R. Li n & R. Qu n

author keywords: Acid pretreatment; Genetic engineering; Lignin; Sugars; Switchgrass
MeSH headings : Carbohydrates / chemical synthesis; Cellulase / chemistry; Panicum / chemistry; Panicum / genetics; Plants, Genetically Modified / chemistry; Sulfuric Acids / chemistry
TL;DR: Genetic engineering resulted in a substantial increase in the ratio of acid soluble lignin:acid insoluble lignIn, which led to considerably increased sugar productions in both pretreatment and enzymatic hydrolysis. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 article

Improvement of Sugar Production from Transgenic Switchgrass with Low-Temperature Alkali Pretreatment

Wang, Z., Xu, J., Pandey, P., Cheng, J. J., Li, R., & Qu, R. (2012, May). ENERGY & FUELS, Vol. 26, pp. 3054–3061.

By: Z. Wang n, J. Xu n, P. Pandey n, J. Cheng n, R. Li n & R. Qu n

UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Sodium hydroxide pretreatment of genetically modified switchgrass for improved enzymatic release of sugars

Bioresource Technology, 110, 364–370.

By: Z. Wang n, R. Li n, J. Xu n, J. Marita*, R. Hatfield*, R. Qu n, J. Cheng n

author keywords: Genetic modification; Lignin; Switchgrass; Sodium hydroxide pretreatment; Sugar
MeSH headings : Biomass; Carbohydrate Metabolism; Hydrolysis; Plants, Genetically Modified; Poaceae / genetics; Poaceae / metabolism; Sodium Hydroxide / chemistry
TL;DR: Results show that down-regulation of 4CL gene promoted enzymatic hydrolysis of plant cell walls following a mild alkali pretreatment, which boosted glucan and xylan conversion efficiency in the best transgenic plants. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2011 journal article

High throughput Agrobacterium-mediated switchgrass transformation

BIOMASS & BIOENERGY, 35(3), 1046–1054.

By: R. Li n & R. Qu n

author keywords: Alamo; Colony; Genetic transformation; GFP; Panicum vertigam L; Performer
TL;DR: The new system substantially improved switchgrass transformation efficiency and will significantly contribute to the genetic improvement of this important biofuel feedstock via biotechnological approach. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Morphological mutants of St. Augustinegrass induced by gamma ray irradiation

PLANT BREEDING, 129(4), 412–416.

By: R. Li n, A. Bruneau n & R. Qu n

author keywords: Gamma ray irradiation; mutagenesis; semi-dwarf; St; Augustinegrass
TL;DR: Mutagenesis by gamma ray irradiation was employed to treat node cuttings and calli for inducing semi-dwarf growth phenotype and altered morphological traits were stable as shown by their growth performance in various locations and conditions. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Selection for freezing tolerance in St. Augustinegrass through somaclonal variation and germplasm evaluation

PLANT BREEDING, 129(4), 417–421.

By: R. Li n, R. Qu n, A. Bruneau n & D. Livingston n

author keywords: Stenotaphrum secundatum; freezing tolerance; germplasm; tissue culture
TL;DR: The experiments revealed that germplasm accession Elm4 was significantly more freezing-Tolerant under a controlled environment than ‘Raleigh’, the current commercially available, most freezing-tolerant cultivar. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Tissue culture-induced morphological somaclonal variation in St. Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze]

PLANT BREEDING, 129(1), 96–99.

By: R. Li n, A. Bruneau n & R. Qu n

author keywords: semi-dwarf; somaclonal variation; St. Augustinegrass; tissue culture
TL;DR: A large scale tissue culture experiment was conducted with St. Augustinegrass, an important turfgrass species for the southern USA, to induce somaclonal variation to enlarge the germplasm pool for breeding efforts and altered traits in the variant lines were stable during vegetative propagation and when grown in different environments. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Protamine-mediated DNA coating remarkably improves bombardment transformation efficiency in plant cells

PLANT CELL REPORTS, 28(2), 213–221.

By: E. Sivamani n, R. DeLong* & R. Qu n

author keywords: DNA coating; DNA delivery; GUS; Plant transformation; Particle bombardment; Protamine
MeSH headings : DNA / chemistry; DNA / genetics; Oryza / metabolism; Plants / genetics; Plasmids / genetics; Protamines / chemistry; Transformation, Genetic / genetics
TL;DR: It is suggested that protamine protects plasmid DNA longer than spermidine when being delivered inside the cells, probably by forming a nano-scale complex, and thus helps improve the efficiency of particle bombardment-mediated plant transformation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Sequence analysis of rice rubi3 promoter gene expression cassettes for improved transgene expression

PLANT SCIENCE, 177(6), 549–556.

By: E. Sivamani n, J. Starmer* & R. Qu n

author keywords: Exon; GUS; IME; rRNA; rubi3 promoter; Translation
TL;DR: Sequence analysis suggests that complementarity between sequences immediately 5′ of a translation initiation codon and the rice 17S rRNA may be responsible for the reduction in protein levels from constructs containing the GUS leader sequence. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Shoot regeneration of dwarf dogwood (Cornus canadensis L.) and morphological characterization of the regenerated plants

PLANT CELL TISSUE AND ORGAN CULTURE, 97(1), 27–37.

By: C. Feng n, R. Qu n, L. Zhou n, D. Xie n & Q. Xiang n

author keywords: Cornus canadensis; Organogenesis; Regeneration; Rhizomes; Tissue culture
TL;DR: An efficient regeneration system is reported for a dwarf dogwood species C. canadensis through organogenesis from rejuvenated leaves, which facilitates developing a genetic transformation system to test candidate genes involved in the developmental divergence of inflorescences in Cornus. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

Activity of the 5 ' regulatory regions of the rice polyubiquitin rubi3 gene in transgenic rice plants as analyzed by both GUS and GFP reporter genes

PLANT CELL REPORTS, 27(10), 1587–1600.

By: J. Lu n, E. Sivamani n, X. Li* & R. Qu n

author keywords: GFP; GUS; intron; rice rubi3 promoter; transgenic plant
MeSH headings : 5' Untranslated Regions / genetics; Base Pairing / genetics; Blotting, Southern; DNA, Bacterial / genetics; Gene Dosage; Gene Expression Regulation, Plant; Genes, Plant; Genes, Reporter; Glucuronidase / genetics; Green Fluorescent Proteins / genetics; Introns / genetics; Oryza / cytology; Oryza / genetics; Plant Leaves / cytology; Plant Leaves / enzymology; Plant Roots / cytology; Plant Roots / enzymology; Plants, Genetically Modified; Plasmids / genetics; Polyubiquitin / genetics; Promoter Regions, Genetic; Regulatory Sequences, Nucleic Acid / genetics
TL;DR: The rubi3 promoter should be an important addition to the arsenal of strong and constitutive promoters for monocot transformation and biotechnology. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Expression of the bacteriophage T4 lysozyme gene in tall fescue confers resistance to gray leaf spot and brown patch diseases

TRANSGENIC RESEARCH, 17(1), 47–57.

By: S. Dong n, H. Shew n, L. Tredway n, J. Lu n, E. Sivamani n, E. Miller n, R. Qu n

author keywords: fungal resistance; Magnaporthe grisea; Rhizoctonia solani; T4 lysozyme; tall fescue
MeSH headings : Bacteriophage T4 / enzymology; Bacteriophage T4 / genetics; Cloning, Molecular; Festuca / enzymology; Festuca / genetics; Festuca / microbiology; Gene Expression; Genes, Viral; Genetic Vectors; Magnaporthe / pathogenicity; Muramidase / genetics; Plant Diseases / genetics; Plant Diseases / microbiology; Plants, Genetically Modified; Rhizobium / genetics; Rhizoctonia / pathogenicity
TL;DR: It is demonstrated that the bacteriophage T4 lysozyme gene confers resistance to both gray leaf spot and brown patch diseases in transgenic tall fescue plants, which may have wide applications in engineered fungal disease resistance in various crops. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

Gene expression enhancement mediated by the 5 ' UTR intron of the rice rubi3 gene varied remarkably among tissues in transgenic rice plants

MOLECULAR GENETICS AND GENOMICS, 279(6), 563–572.

By: J. Lu n, E. Sivamani n, K. Azhakanandam n, P. Samadder n, X. Li* & R. Qu n

author keywords: IME; intron; rubi3 gene promoter; transgenic rice; translation
MeSH headings : 5' Untranslated Regions / chemistry; Gene Expression Regulation, Plant; Genes, Plant; Genes, Reporter; Introns; Oryza / genetics; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Polyubiquitin / genetics; Promoter Regions, Genetic; Protein Biosynthesis; RNA, Messenger / metabolism; Tissue Distribution; Transformation, Genetic
TL;DR: Substantial variation in the magnitudes of intron-mediated enhancement of gene expression (IME) among tissues in rice plants is revealed and the importance of using transgenic plants for IME studies is revealed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Transcriptional and post-transcriptional enhancement of gene expression by the 5 ' UTR intron of rice rubi3 gene in transgenic rice cells

MOLECULAR GENETICS AND GENOMICS, 279(4), 429–439.

By: P. Samadder n, E. Sivamani n, J. Lu n, X. Li* & R. Qu n

author keywords: EJC; exon; gene expression; IME; nuclear run-on
MeSH headings : 5' Untranslated Regions; Base Sequence; DNA Primers / genetics; Gene Expression Regulation, Plant; Genes, Plant; Genes, Reporter; Introns; Oryza / genetics; Oryza / metabolism; Plant Proteins / genetics; Plants, Genetically Modified; Polyubiquitin / genetics; RNA Processing, Post-Transcriptional; RNA Splicing; RNA, Plant / genetics; RNA, Plant / metabolism; Transcription, Genetic
TL;DR: Results demonstrated transcriptional enhancement by a plant intron, but suggested that post-transcriptional event(s) be the major source of IME, implying a strong role of the intron in RNA processing and/or export. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2007 article

Amplicon-plus Targeting Technology (APTT) for rapid production of a highly unstable vaccine protein in tobacco plants (vol 64, pg 619, 2007)

Azhakanandam, K., Weissinger, S. M., Nicholson, J. S., Qu, R., & Weissinger, A. K. (2007, July). PLANT MOLECULAR BIOLOGY, Vol. 64, pp. 619–619.

Source: Web Of Science
Added: August 6, 2018

2007 journal article

Amplicon-plus targeting technology (APTT) for rapid production of a highly unstable vaccine protein in tobacco plants

Plant Molecular Biology, 63(3), 393–404.

MeSH headings : Biotechnology; Capsid Proteins / biosynthesis; Capsid Proteins / genetics; Capsid Proteins / immunology; Gene Expression; Genetic Vectors; Lambdapapillomavirus / genetics; Lambdapapillomavirus / immunology; Plants, Genetically Modified; RNA, Small Interfering / genetics; Recombinant Proteins / biosynthesis; Recombinant Proteins / genetics; Recombinant Proteins / immunology; Rhizobium / genetics; Tobacco / genetics; Vaccines, Subunit / biosynthesis; Vaccines, Subunit / genetics; Viral Vaccines / biosynthesis; Viral Vaccines / genetics
TL;DR: The good yield and short interval from inoculation to harvest characteristic of APTT, combined with the potential for high-throughput achieved by use of the agrospray inoculation protocol, make this system a very promising technology for producing high value recombinant proteins, especially those known to be highly labile, in plants for a wide range of applications. (via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018

2007 journal article

Resistance of transgenic tall fescue to two major fungal diseases

PLANT SCIENCE, 173(5), 501–509.

author keywords: alfalfa AGLU1 gene; dermaseptin SI gene; fungal resistance; Magnaporthe grisea; Rhizoctonia solani; rice Pi9 gene
TL;DR: Of 15 T0 transgenic plants examined, 6 showed high levels of resistance to M. grisea, and 3 had enhanced resistance to R. solani, and the resistance in most of these cases was highly significant. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Expression enhancement of a rice polyubiquitin gene promoter

PLANT MOLECULAR BIOLOGY, 60(2), 225–239.

By: E. Sivamani n & R. Qu n

author keywords: exon; gene expression; GUS; IME; intron; polyubiquitin; promoter; rice
MeSH headings : 5' Untranslated Regions; Base Sequence; Blotting, Western; DNA Primers; Gene Expression Regulation, Plant; Glucuronidase / genetics; Introns; Luciferases / genetics; Oryza / genetics; Polyubiquitin / genetics; Promoter Regions, Genetic; Reverse Transcriptase Polymerase Chain Reaction
TL;DR: The resultant 5′ regulatory sequence, consisting of the rubi3 promoter, 5′ UTR exon and intron, and the mutated first 9 nt coding sequence, has an activity nearly 90-fold greater than the rubu3 promoter only (without the 5′UTR intron), and 2.2-foldgreater than the maize Ubi1 gene promoter (including its 5″ UTR introns). (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Improved plant regeneration and in vitro somatic embryogenesis of St Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze]

PLANT BREEDING, 125(1), 52–56.

By: R. Li n, A. Bruneau n & R. Qu n

author keywords: Stenotaphrum secundatum; benzyladenine; immature embryo; regeneration; scanning electron microscopy; somatic embryogenesis
TL;DR: Scanning electron microscopy revealed that plant regeneration of St Augustinegrass is via somatic embryogenesis, and the addition of benzyladenine in the callus induction/subculture medium enhances callus regeneration ability and does not harmCallus induction for immature embryos. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Agrobacterium-Mediated transformation of common bermudagrass (Cynodon dactylon)

PLANT CELL TISSUE AND ORGAN CULTURE, 83(2), 223–229.

By: L. Li n, R. Li n, S. Fei* & R. Qu n

author keywords: Agrobacterium; bermudagrass; embryogenic callus; suspension; transgenic; turfgrass
TL;DR: Common bermudagrass, Cynodon dactylon, is a widely used warm-season turf and forage species in the temperate and tropical regions of the world and has been able to transform the species using Agrobacterium-mediated approach. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Evidence for non-proteinaceous inhibitor(s) of beta-glucuronidase in wheat (Triticum aestivum L.) leaf and root tissues

PLANT CELL TISSUE AND ORGAN CULTURE, 82(1), 11–17.

By: A. Bahieldin*, H. Eissa*, H. Mahfouz*, W. Dyer*, M. Madkour* & R. Qu n

author keywords: ss-glucuronidase inhibitor; genetic transformation; GUS; transgene; wheat
TL;DR: Florometric and histochemical assays performed in the presence of wheat tissue extracts indicated that wheat leaf and root tissues contain inhibitor(s) of β-glucuronidase activity, but pollen grains contain much lower concentrations, and characterizations indicated that the inhibitor is of low molecular weight and is non-proteinaceous. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

High efficiency transformation of tall fescue with Agrobacterium tumefaciens

PLANT SCIENCE, 168(6), 1453–1458.

By: S. Dong n & R. Qu n

author keywords: 2,4-D; Agrobacterium tumefaciens; genetic engineering; tall fescue; transgenic plants
TL;DR: The whole process from callus induction to transplanting green plantlets to the soil takes about six months, significantly shorter than the suspension culture approach often used in tall fescue transformation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Selective elimination of perennial ryegrass by activation of a pro-herbicide through engineering E-coli argE gene

MOLECULAR BREEDING, 15(4), 339–347.

By: X. Chen n, W. Yang n, E. Sivamani n, A. Bruneau n, B. Wang n & R. Qu n

author keywords: argE; N-acetyl-phosphinothricin; perennial ryegrass; phosphinothricin; summer persistence; transgenics
TL;DR: This approach provides a means to selectively remove a group of transgenic plants without affecting other plants growing with them and to cause self elimination of the plants. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Development of highly regenerable callus lines and biolistic transformation of turf-type common bermudagrass [Cynodon dactylon (L.) Pers.]

PLANT CELL REPORTS, 22(6), 403–407.

By: L. Li n & R. Qu n

author keywords: bermudagrass; embryogenic; plant regeneration; transgenic; turfgrass
MeSH headings : Biolistics / methods; Biotechnology / methods; Cell Line; Cynodon / embryology; Cynodon / genetics; Cynodon / physiology; Flowers / genetics; Flowers / physiology; Hygromycin B / isolation & purification; Regeneration
TL;DR: Nine long-term, highly regenerable callus lines were subsequently established, of which six were used for biolistic transformation, and three transgenic events displayed resistance to the herbicide glufosinate, and one showed β-glucuronidase activity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Lead uptake by roots of four turfgrass species in hydroponic cultures

HortScience, 38(4), 623–626.

By: R. Qu, D. Li, R. Du & R. Qu

UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

2002 journal article

In vitro somatic embryogenesis in turf-type bermudagrass: roles of abscisic acid and gibberellic acid, and occurrence of secondary somatic embryogenesis

PLANT BREEDING, 121(2), 155–158.

By: L. Li n & R. Qu n

author keywords: Cynodon dactylon x Cynodon transvaalensis; hybrid turfgrass; somatic embryo genesis; BAP supplement
TL;DR: This study found that secondary and repetitive somatic embryogenesis, which is rarely reported in monocots, was observed in common bermudagrass (Cynodon dactylon, cv. ‘Savannah’), and a full course of such a development was captured by a periodical microphotography. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2001 journal article

Effects of cultivar, explant treatment, and medium supplements on callus induction and plantlet regeneration in perennial ryegrass

International Turfgrass Society Research Journal, 9(2001), 152–156.

By: D. Bradley, A. Bruneau & R. Qu

Source: NC State University Libraries
Added: August 6, 2018

2001 journal article

Factors influencing tissue culture responses of mature seeds and immature embryos in turf-type tall fescue

PLANT BREEDING, 120(3), 239–242.

By: Y. Bai* & R. Qu*

author keywords: Festuca arundinacea; 6-benzylaminopurine; casein hydrolysate; L-proline; myo-inositol; thidiazuron
TL;DR: By simply slicing the mature seeds into two halves longitudinally, the callus induction frequencies, as well as the corresponding overall plant regeneration frequencies, were increased approximately three- to six-fold in all three combinations of 2,4-dichlorophenoxyacetic acid and BAP incallus induction media. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2001 journal article

Genetic transformation of elite cultivars of turf-type tall fescue (Festuca arundinacea)

International Turfgrass Society Research Journal, 9(2001), 129–136.

By: Y. Bai & R. Qu

Source: NC State University Libraries
Added: August 6, 2018

2001 journal article

Improved young inflorescence culture and regeneration of ifway bermudagrass (Cynodon transvaalensis x C. dactylon)

International Turfgrass Society Research Journal, 9(2001), 198–201.

By: R. Qu & A. Chaudhury

Source: NC State University Libraries
Added: August 6, 2018

2001 journal article

Scanning electron microscopic study on in vitro somatic embryogenesis in perennial ryegrass and tall fescue

International Turfgrass Society Research Journal, 9(2001), 146–151.

By: D. Bradley, A. Bruneau & R. Qu

Source: NC State University Libraries
Added: August 6, 2018

2000 journal article

An evaluation of callus induction and plant regeneration in twenty-five turf-type tall fescue (Festuca arundinacea Schreb.) cultivars

GRASS AND FORAGE SCIENCE, 55(4), 326–330.

By: Y. Bai n & R. Qu n

TL;DR: Twentyfive turf-type tall fescue cultivars, including many elite ones released recently, were evaluated for their callus induction and plant regeneration responses and significant differences were observed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2000 journal article

Concentration effects of dicamba on shoot regeneration in wheat

PLANT BREEDING, 119(5), 437–439.

By: A. Bahieldin*, W. Dyer* & R. Qu n

author keywords: Triticum aestivum; dicamba; shoot regeneration; tissue culture
TL;DR: It was found that a dicamba concentration of 0.02 mg/l was more favourable for wheat regeneration than the levels reported previously, while the enhancement in regeneration rate was variety dependent. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2000 journal article

Improved biomass productivity and water use efficiency under water deficit conditions in transgenic wheat constitutively expressing the barley HVA1 gene

PLANT SCIENCE, 155(1), 1–9.

By: E. Sivamani*, A. Bahieldin*, J. Wraith*, T. Al-Niemi*, W. Dyer*, T. Ho*, R. Qu*

author keywords: barley HVA1 gene; biomass productivity; LEA protein; water deficit; water use efficiency; wheat transformation
TL;DR: Results of this study indicate that growth characteristics were improved in transgenic wheat plants constitutively expressing the barley HVA1 gene in response to soil water deficit. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
6. Clean Water and Sanitation (OpenAlex)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2000 journal article

Resistance to wheat streak mosaic virus in transgenic wheat expressing the viral replicase (NIb) gene

MOLECULAR BREEDING, 6(5), 469–477.

By: E. Sivamani*, C. Brey, W. Dyer, L. Talbert & R. Qu

author keywords: pathogen-derived resistance; replicase-mediated resistance; wheat transformation; WSMV
TL;DR: Six independent transgenic plant lines were analyzed for transgene expression and for resistance to mechanical inoculation of wheat streak mosaic virus at R3 or R4 generation and four out of the six lines showed various degree of resistance to WSMV. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2000 journal article

Somatic embryogenesis and plant regeneration of turf-type bermudagrass: Effect of 6-benzyladenine in callus induction medium

PLANT CELL TISSUE AND ORGAN CULTURE, 60(2), 113–120.

By: A. Chaudhury n & R. Qu n

author keywords: Cynodon dactylon; Cynodon dactylon x Cynodon transvaalensis; cytokinin; MS medium; plant tissue culture
TL;DR: Tissue culture responses of young inflorescences of a hybrid bermudagrass cultivar `Tifgreen' (Cynodon dactylon×Cynodocus transvaalensis) and a common bermUDagrasses cultivar`Savannah' (cynodondactylon) were investigated and formed a compact, nodular embryogenic structure which was highly regenerable and morphologically normal. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2000 journal article

Thidiazuron promotes in vitro regeneration of wheat and barley

In Vitro Cellular & Developmental Biology. Plant, 36(3), 207–210.

By: X. Shan*, D. Li* & R. Qu*

TL;DR: Thidiazuron (TDZ) is a substituted phenylurea which has been shown to be an efficacious regulator of in vitro morphogenesis of many dicot plant species and was suggested to be among the best for in vitro regeneration of wheat and barley. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

1999 journal article

Nuclear DNA content of thirteen turfgrass species by flow cytometry

Crop Science, 39(5), 1518–1521.

By: K. Arumuganathan, S. Tallury, M. Fraser, A. Bruneau & R. Qu

Source: NC State University Libraries
Added: August 6, 2018

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